LOCUS       sequence-207990-        5978 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Luciferase reporter plasmid to monitor transcriptional activity of 
            canine ITGA6-promoter (1kb fragment).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5978)
  AUTHORS   Zhang K, Myllymaki SM, Gao P, Devarajan R, Kytola V, Nykter M, Wei 
            GH, Manninen A
  TITLE     Oncogenic K-Ras upregulates ITGA6 expression via FOSL1 to induce 
            anoikis resistance and synergizes with alphaV-Class integrins to 
            promote EMT.
  JOURNAL   Oncogene. 2017 Oct 12;36(41):5681-5694. doi: 10.1038/onc.2017.177. 
            Epub 2017 Jun 12.
  PUBMED    28604746
REFERENCE   2  (bases 1 to 5978)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/onc.2017.177"; journalName: "Oncogene"; date: "2017-10-12- 
            12"; volume: "36"; issue: "41"; pages: "5681-5694"
COMMENT     Sequence Label: pGL3-ITGA6-1k-Basic-LUC
FEATURES             Location/Qualifiers
     source          1..5978
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     regulatory      1242..1251
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1248..2900
                     /codon_start=1
                     /gene="luc+"
                     /product="firefly luciferase"
                     /label=luciferase
                     /note="enhanced luc+ version of the luciferase gene"
                     /translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA
                     HIEVDITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP
                     ANDIYNERELLNSMGISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS
                     MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA
                     RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK
                     IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY
                     GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS
                     GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL
                     LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV
                     FVDEVPKGLTGKLDARKIREILIKAKKGGKIAV"
     primer_bind     complement(1314..1332)
                     /label=LucNrev
                     /note="Firefly luciferase, reverse primer"
     polyA_signal    2941..3062
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2952..2971)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     3006..3025
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     complement(3310..3327)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(3481..4069)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(3561..3580)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(4240..5031,5032..5100))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     4863..4882
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(5101..5205)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      5232..5687
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5319..5338)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5529..5550
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     polyA_signal    5818..5866
                     /note="synthetic polyadenylation signal"
     misc_feature    5880..5971
                     /label=pause site
                     /note="RNA polymerase II transcriptional pause signal from 
                     the human alpha-2 globin gene"
     primer_bind     5920..5939
                     /label=RVprimer3
                     /note="pGL3 vector, forward primer"
ORIGIN
        1 ggtaccgagc tcttacgcgt gctagcccgg gctcgagaat ttgtattgta gcagtatgtg
       61 tactttttac ttttttattt tttattttta tttatttatt tttaaatttt tatttattta
      121 tgatagtcac acagagagag aggcagagac acaggcagaa ggagaagcag gctccatgca
      181 ctgggacccc gacgtgggac tcgatcccgg gtctccagga tcacgccctg ggccaaaggc
      241 aggtgccaaa ccgctgcgcc acccaggtat ccctattttt tttttttttt taagatttat
      301 ttattcatga tagacacaca cagagggaga gagagaggca gagacaggca gagggagaag
      361 tgggttccat gcagggagcc ggatgtggga cactcgatcc caggactcca ggatcacacc
      421 ctgggcccaa ggcaggtgct aaactgctga gccacccagg gatcccctat atatactttt
      481 tttttttttt actttttaat taacctattg cataacaaga tctagcagag ggtctaccta
      541 ataaccactg taatcgaagg tattgtaaca tctatgatgt gatgtaaaaa aaaccctgtg
      601 gtttctactg gagaataagt cataggtcct taccctactg ccactgtgtt gtgttgccta
      661 cattcataat ggaaggaaat gctaaatgtt agaggcttag tgaaatgaaa aatgtaaatt
      721 ttttgtcacc gaaatctcac agtacccctg aattctctct cccctgccca agaactcttg
      781 ccaatagtta ccccagaggt caacacctca ctgacctcac tggaggccac accccatgct
      841 ctggaggcag gaggctttag accacccaca cagagctctg agctgcctcc tggccaggat
      901 ctagtcctgc gaatctgggg gcaggaaaga agggcccccg agcccatagg tatcaacttt
      961 ttttttttta atattttatt tattcatgag agacacagag aggcagagac ccaggcagag
     1021 ggagaagcag gccccatgca gggagccgac gcgggactcg atcccaggac cccaggatca
     1081 cgccctgggc tgacggcaga tgctcaaccg ctgagccacc aggtgcccgg aaccctctta
     1141 atcccccata tcctttacag tcgaggagct gctcctgagc ttcgttgcac ccagaaacca
     1201 agttttgaac tgaagcttgg cattccggta ctgttggtaa agccaccatg gaagacgcca
     1261 aaaacataaa gaaaggcccg gcgccattct atccgctgga agatggaacc gctggagagc
     1321 aactgcataa ggctatgaag agatacgccc tggttcctgg aacaattgct tttacagatg
     1381 cacatatcga ggtggacatc acttacgctg agtacttcga aatgtccgtt cggttggcag
     1441 aagctatgaa acgatatggg ctgaatacaa atcacagaat cgtcgtatgc agtgaaaact
     1501 ctcttcaatt ctttatgccg gtgttgggcg cgttatttat cggagttgca gttgcgcccg
     1561 cgaacgacat ttataatgaa cgtgaattgc tcaacagtat gggcatttcg cagcctaccg
     1621 tggtgttcgt ttccaaaaag gggttgcaaa aaattttgaa cgtgcaaaaa aagctcccaa
     1681 tcatccaaaa aattattatc atggattcta aaacggatta ccagggattt cagtcgatgt
     1741 acacgttcgt cacatctcat ctacctcccg gttttaatga atacgatttt gtgccagagt
     1801 ccttcgatag ggacaagaca attgcactga tcatgaactc ctctggatct actggtctgc
     1861 ctaaaggtgt cgctctgcct catagaactg cctgcgtgag attctcgcat gccagagatc
     1921 ctatttttgg caatcaaatc attccggata ctgcgatttt aagtgttgtt ccattccatc
     1981 acggttttgg aatgtttact acactcggat atttgatatg tggatttcga gtcgtcttaa
     2041 tgtatagatt tgaagaagag ctgtttctga ggagccttca ggattacaag attcaaagtg
     2101 cgctgctggt gccaacccta ttctccttct tcgccaaaag cactctgatt gacaaatacg
     2161 atttatctaa tttacacgaa attgcttctg gtggcgctcc cctctctaag gaagtcgggg
     2221 aagcggttgc caagaggttc catctgccag gtatcaggca aggatatggg ctcactgaga
     2281 ctacatcagc tattctgatt acacccgagg gggatgataa accgggcgcg gtcggtaaag
     2341 ttgttccatt ttttgaagcg aaggttgtgg atctggatac cgggaaaacg ctgggcgtta
     2401 atcaaagagg cgaactgtgt gtgagaggtc ctatgattat gtccggttat gtaaacaatc
     2461 cggaagcgac caacgccttg attgacaagg atggatggct acattctgga gacatagctt
     2521 actgggacga agacgaacac ttcttcatcg ttgaccgcct gaagtctctg attaagtaca
     2581 aaggctatca ggtggctccc gctgaattgg aatccatctt gctccaacac cccaacatct
     2641 tcgacgcagg tgtcgcaggt cttcccgacg atgacgccgg tgaacttccc gccgccgttg
     2701 ttgttttgga gcacggaaag acgatgacgg aaaaagagat cgtggattac gtcgccagtc
     2761 aagtaacaac cgcgaaaaag ttgcgcggag gagttgtgtt tgtggacgaa gtaccgaaag
     2821 gtcttaccgg aaaactcgac gcaagaaaaa tcagagagat cctcataaag gccaagaagg
     2881 gcggaaagat cgccgtgtaa ttctagagtc ggggcggccg gccgcttcga gcagacatga
     2941 taagatacat tgatgagttt ggacaaacca caactagaat gcagtgaaaa aaatgcttta
     3001 tttgtgaaat ttgtgatgct attgctttat ttgtaaccat tataagctgc aataaacaag
     3061 ttaacaacaa caattgcatt cattttatgt ttcaggttca gggggaggtg tgggaggttt
     3121 tttaaagcaa gtaaaacctc tacaaatgtg gtaaaatcga taaggatccg tcgaccgatg
     3181 cccttgagag ccttcaaccc agtcagctcc ttccggtggg cgcggggcat gactatcgtc
     3241 gccgcactta tgactgtctt ctttatcatg caactcgtag gacaggtgcc ggcagcgctc
     3301 ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc
     3361 agctcactca aaggcggtaa tacggttatc cacagaatca ggggataacg caggaaagaa
     3421 catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt
     3481 tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg
     3541 gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg
     3601 ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag
     3661 cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc
     3721 caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa
     3781 ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg
     3841 taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc
     3901 taactacggc tacactagaa gaacagtatt tggtatctgc gctctgctga agccagttac
     3961 cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg
     4021 tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt
     4081 gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt
     4141 catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa
     4201 atcaatctaa agtatatatg agtaaacttg gtctgacagt taccaatgct taatcagtga
     4261 ggcacctatc tcagcgatct gtctatttcg ttcatccata gttgcctgac tccccgtcgt
     4321 gtagataact acgatacggg agggcttacc atctggcccc agtgctgcaa tgataccgcg
     4381 agacccacgc tcaccggctc cagatttatc agcaataaac cagccagccg gaagggccga
     4441 gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag tctattaatt gttgccggga
     4501 agctagagta agtagttcgc cagttaatag tttgcgcaac gttgttgcca ttgctacagg
     4561 catcgtggtg tcacgctcgt cgtttggtat ggcttcattc agctccggtt cccaacgatc
     4621 aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc
     4681 gatcgttgtc agaagtaagt tggccgcagt gttatcactc atggttatgg cagcactgca
     4741 taattctctt actgtcatgc catccgtaag atgcttttct gtgactggtg agtactcaac
     4801 caagtcattc tgagaatagt gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg
     4861 ggataatacc gcgccacata gcagaacttt aaaagtgctc atcattggaa aacgttcttc
     4921 ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc agttcgatgt aacccactcg
     4981 tgcacccaac tgatcttcag catcttttac tttcaccagc gtttctgggt gagcaaaaac
     5041 aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt gaatactcat
     5101 actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata
     5161 catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa
     5221 agtgccacct gacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg
     5281 cagcgtgacc gctacacttg ccagcgccct agcgcccgct cctttcgctt tcttcccttc
     5341 ctttctcgcc acgttcgccg gctttccccg tcaagctcta aatcgggggc tccctttagg
     5401 gttccgattt agtgctttac ggcacctcga ccccaaaaaa cttgattagg gtgatggttc
     5461 acgtagtggg ccatcgccct gatagacggt ttttcgccct ttgacgttgg agtccacgtt
     5521 ctttaatagt ggactcttgt tccaaactgg aacaacactc aaccctatct cggtctattc
     5581 ttttgattta taagggattt tgccgatttc ggcctattgg ttaaaaaatg agctgattta
     5641 acaaaaattt aacgcgaatt ttaacaaaat attaacgctt acaatttgcc attcgccatt
     5701 caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat tacgccagcc
     5761 caagctacca tgataagtaa gtaatattaa ggtacgggag gtacttggag cggccgcaat
     5821 aaaatatctt tattttcatt acatctgtgt gttggttttt tgtgtgaatc gatagtacta
     5881 acatacgctc tccatcaaaa caaaacgaaa caaaacaaac tagcaaaata ggctgtcccc
     5941 agtgcaagtg caggtgccag aacatttctc tatcgata
//
