LOCUS       sequence-207991-        5682 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  To test the effect of sequence on TDP43 droplet properties.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5682)
  TITLE     Rajat Rohatgi TDP43 plasmids
REFERENCE   2  (bases 1 to 5682)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pHBS981 TDP43 RRM-GFP G348D
FEATURES             Location/Qualifiers
     source          1..5682
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        30..48
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     30..47
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     protein_bind    103..127
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     regulatory      146..155
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     protein_bind    complement(1660..1684)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     polyA_signal    1729..1863
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(1753..1772)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     1807..1826
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     complement(1976..1996)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(1976..1994)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(2015..2031)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2015..2031)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(2028..2050)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    2039..2055
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(2063..2069,2070..2087,2088..2093))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2108..2129
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(2246..2263)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(2417..3005)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2497..2516)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(3176..3967,3968..4036))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     3799..3818
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(4037..4141)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(4167..4622)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4304..4325)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     4516..4535
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        4693..5677
                     /label=CMV IE94 promoter
                     /note="enhancer/promoter region of simian cytomegalovirus 
                     major immediate early transcription unit IE94"
ORIGIN
        1 ttctgcctgg ggacgtcgga gcaagcttga tttaggtgac actatagaat acaagctact
       61 tgttcttttt gcaggatccc atcgattcga attcaaggat caacaagttt gtacaaaaaa
      121 gcaggctggc gccggtacca ccggtgccac catgggatcc atgtctgaat atattcgggt
      181 aaccgaagat gagaacgatg agcccattga aataccatcg gaagacgatg ggacggtgct
      241 gctctccacg gttacagccc agtttccagg ggcgtgtggg cttcgctaca ggaatccagt
      301 gtctcagtgt atgagaggtg tccggctggt agaaggaatt ctgcatgccc cagatgctgg
      361 ctggggaaat ctggtgtatg ttgtcaacta tccaaaagat aacaaaagaa aaatggatga
      421 gacagatgct tcatcagcag tgaaagtgaa aagagcagtc cagaaaacat ccagtaaagg
      481 agaggaacta tttacaggta ctgtgcctat caaagtcgag ctggacggcg acgtaaacgg
      541 ccacaagttc agcgtgcgtg gcgagggcga gggcgatgcc accgagggca agctgaccct
      601 gaagttcatc tgcaccaccg gcaagctgcc ggtgccgtgg ccgaccctcg tgaccaccct
      661 gacctacggc gtgcagtgct tcagccgcta cccggaccac atgaagcgtc acgacttctt
      721 caagtccgcc atgccggaag gctacgtcca ggagcgcacc atcgagttca aggacgacgg
      781 cacctacaag acccgcgcgg aggtgaagtt cgagggcgac accctggtga accgcatcga
      841 gctgaagggc aacgacttca aggaggacgg caacatcctg ggtcacaagc tggagtacaa
      901 ccacaacagc cacaacgtcc gtatcgaagc cgacaagcag aagaacggca tcaaggctaa
      961 cttcaagatc cgccacaacg ttgaggacgg cagccagcag gaagccgacc acaaacagca
     1021 gaacaccccg atcggcgacg gcccggtgcg cctgccggac aaccactatc tgagcaccca
     1081 gaccacgctg agcaaagacc cgaacgagaa gcgcgatcac atggtcctga aggagttcgt
     1141 gacggcagcc ggtatcacta aaggtgaaga tgaaagtgga tccaatgccg aacctaagca
     1201 caatagcaat agacagttag aaagaagtgg aagatttggt ggtaatccag gtggctttgg
     1261 gaatcagggt ggatttggta atagcagagg gggtggagct ggtttgggaa acaatcaagg
     1321 tagtaatatg ggtggtggga tgaactttgg tgcgttcagc attaatccag ccatgatggc
     1381 tgccgcccag gcagcactac agagcagttg gggtatgatg ggcatgttag ccagccagca
     1441 gaaccagtca gacccatcgg gtaataacca aaaccaaggc aacatgcaga gggagccaaa
     1501 ccaggccttc ggttctggaa ataactctta tagtggctct aattctggtg cagcaattgg
     1561 ttggggatca gcatccaatg cagggtcggg cagtggtttt aatggaggct ttggctcaag
     1621 catggattct aagtcttctg gctggggaat gtagactaga cccagctttc ttgtacaaag
     1681 tggttgatcc tctcgagcct ctagaactat agtgagtcgt attacgtaga tccagacatg
     1741 ataagataca ttgatgagtt tggacaaacc acaactagaa tgcagtgaaa aaaatgcttt
     1801 atttgtgaaa tttgtgatgc tattgcttta tttgtaacca ttataagctg caataaacaa
     1861 gttaacaaca acaattgcat tcattttatg tttcaggttc agggggaggt gtgggaggtt
     1921 ttttaattcg cggccgcggc gccaatgcat tgggcccggt acccagcttt tgttcccttt
     1981 agtgagggtt aattgcgcgc ttggcgtaat catggtcata gctgtttcct gtgtgaaatt
     2041 gttatccgct cacaattcca cacaacatac gagccgggag cataaagtgt aaagcctggg
     2101 gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg ctcactgccc gctttccagt
     2161 cgggaaacct gtcgtgccag ctgcattaat gaatcggcca acgcgcgggg agaggcggtt
     2221 tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc
     2281 tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatcagggg
     2341 ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg
     2401 ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac
     2461 gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg
     2521 gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct
     2581 ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat ctcagttcgg
     2641 tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct
     2701 gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac
     2761 tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt
     2821 tcttgaagtg gtggcctaac tacggctaca ctagaagaac agtatttggt atctgcgctc
     2881 tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca
     2941 ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat
     3001 ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac
     3061 gttaagggat tttggtcatg agattatcaa aaaggatctt cacctagatc cttttaaatt
     3121 aaaaatgaag ttttaaatca atctaaagta tatatgagta aacttggtct gacagttacc
     3181 aatgcttaat cagtgaggca cctatctcag cgatctgtct atttcgttca tccatagttg
     3241 cctgactccc cgtcgtgtag ataactacga tacgggaggg cttaccatct ggccccagtg
     3301 ctgcaatgat accgcgagac ccacgctcac cggctccaga tttatcagca ataaaccagc
     3361 cagccggaag ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc atccagtcta
     3421 ttaattgttg ccgggaagct agagtaagta gttcgccagt taatagtttg cgcaacgttg
     3481 ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct tcattcagct
     3541 ccggttccca acgatcaagg cgagttacat gatcccccat gttgtgcaaa aaagcggtta
     3601 gctccttcgg tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg
     3661 ttatggcagc actgcataat tctcttactg tcatgccatc cgtaagatgc ttttctgtga
     3721 ctggtgagta ctcaaccaag tcattctgag aatagtgtat gcggcgaccg agttgctctt
     3781 gcccggcgtc aatacgggat aataccgcgc cacatagcag aactttaaaa gtgctcatca
     3841 ttggaaaacg ttcttcgggg cgaaaactct caaggatctt accgctgttg agatccagtt
     3901 cgatgtaacc cactcgtgca cccaactgat cttcagcatc ttttactttc accagcgttt
     3961 ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga
     4021 aatgttgaat actcatactc ttcctttttc aatattattg aagcatttat cagggttatt
     4081 gtctcatgag cggatacata tttgaatgta tttagaaaaa taaacaaata ggggttccgc
     4141 gcacatttcc ccgaaaagtg ccacctaaat tgtaagcgtt aatattttgt taaaattcgc
     4201 gttaaatttt tgttaaatca gctcattttt taaccaatag gccgaaatcg gcaaaatccc
     4261 ttataaatca aaagaataga ccgagatagg gttgagtgtt gttccagttt ggaacaagag
     4321 tccactatta aagaacgtgg actccaacgt caaagggcga aaaaccgtct atcagggcga
     4381 tggcccacta cgtgaaccat caccctaatc aagttttttg gggtcgaggt gccgtaaagc
     4441 actaaatcgg aaccctaaag ggagcccccg atttagagct tgacggggaa agccggcgaa
     4501 cgtggcgaga aaggaaggga agaaagcgaa aggagcgggc gctagggcgc tggcaagtgt
     4561 agcggtcacg ctgcgcgtaa ccaccacacc cgccgcgctt aatgcgccgc tacagggcgc
     4621 gtcccattcg ccattcaggc tgcgcaactg ttgggaaggg cgatcggtgc gggcctcttc
     4681 gctattacgc cagtcgaccg ccaattcaat atggcgtata tggactcatg ccaattcaat
     4741 atggtggatc tggacctgtg ccaattcaat atggcgtata tggactcgtg ccaattcaat
     4801 atggtggatc tggaccccag ccaattcaat atggcggact tggcaccatg ccaattcaat
     4861 atggcggacc tggcactgtg ccaactgggg aggggtctac ttggcacggt gccaagtttg
     4921 aggaggggtc ttggccctgt gccaagtccg ccatattgaa ttggcatggt gccaataatg
     4981 gcggccatat tggctatatg ccaggatcaa tatataggca atatccaata tggccctatg
     5041 ccaatatggc tattggccag gttcaatact atgtattggc cctatgccat atagtattcc
     5101 atatatgggt tttcctattg acgtagatag cccctcccaa tgggcggtcc catataccat
     5161 atatggggct tcctaatacc gcccatagcc actcccccat tgacgtcaat ggtctctata
     5221 tatggtcttt cctattgacg tcatatgggc ggtcctattg acgtatatgg cgcctccccc
     5281 attgacgtca attacggtaa atggcccgcc tggctcaatg cccattgacg tcaataggac
     5341 cacccaccat tgacgtcaat gggatggctc attgcccatt catatccgtt ctcacgcccc
     5401 ctattgacgt caatgacggt aaatggccca cttggcagta catcaatatc tattaatagt
     5461 aacttggcaa gtacattact attggaagta cgccagggta cattggcagt actcccattg
     5521 acgtcaatgg cggtaaatgg cccgcgatgg ctgccaagta catccccatt gacgtcaatg
     5581 gggaggggca atgacgcaaa tgggcgttcc attgacgtaa atgggcggta ggcgtgccta
     5641 atgggaggtc tatataagca atgctcgttt agggaaccgc ca
//
