LOCUS       sequence-207995-       14487 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Arabidopsis transformation, expresses HDT1 promoter/GUS fusion to 
            study expression pattern in roots.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 14487)
  AUTHORS   Li H, Torres-Garcia J, Latrasse D, Benhamed M, Schilderink S, Zhou 
            W, Kulikova O, Hirt H, Bisseling T
  TITLE     Plant-Specific Histone Deacetylases HDT1/2 Regulate GIBBERELLIN 
            2-OXIDASE2 Expression to Control Arabidopsis Root Meristem Cell 
            Number.
  JOURNAL   Plant Cell. 2017 Sep;29(9):2183-2196. doi: 10.1105/tpc.17.00366. 
            Epub 2017 Aug 30.
  PUBMED    28855334
REFERENCE   2  (bases 1 to 14487)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1105/tpc.17.00366"; journalName: "Plant Cell"; date: "2017-09"; 
            volume: "29"; issue: "9"; pages: "2183-2196"
COMMENT     Sequence Label: pHDT1:GUS
FEATURES             Location/Qualifiers
     source          1..14487
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(244..873)
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     misc_feature    2204..2228
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     primer_bind     2295..2311
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     2295..2311
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     protein_bind    2331..2351
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     protein_bind    2412..2436
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(3965..3989)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             3997..5805
                     /codon_start=1
                     /gene="uidA (or gusA) from E. coli"
                     /product="beta-glucuronidase"
                     /label=GUS
                     /translation="MLRPVETPTREIKKLDGLWAFSLDRENCGIDQRWWESALQESRAI
                     AVPGSFNDQFADADFRNYAGNVWYQREVFIPKGWAGQRIVLRFDAVTHYGKVWVNNQEV
                     MEHQGGYTPFEADVTPYVIAGKSVRITVCVNNELNWQTIPPGMVITDENGKKKQSYFHD
                     FFNYAGIHRSVMLYTTPNTWVDDITVVTHVAQDCNHASVDWQVVANGDVSVELRDADQQ
                     VVATGQGTSGTLQVVNPHLWQPGEGYLYELCVTAKSQTECDIYPLRVGIRSVAVKGEQF
                     LINHKPFYFTGFGRHEDADLRGKGFDNVLMVHDHALMDWIGANSYRTSHYPYAEEMLDW
                     ADEHGIVVIDETAAVGFNLSLGIGFEAGNKPKELYSEEAVNGETQQAHLQAIKELIARD
                     KNHPSVVMWSIANEPDTRPQGAREYFAPLAEATRKLDPTRPITCVNVMFCDAHTDTISD
                     LFDVLCLNRYYGWYVQSGDLETAEKVLEKELLAWQEKLHQPIIITEYGVDTLAGLHSMY
                     TDMWSEEYQCAWLDMYHRVFDRVSAVVGEQVWNFADFATSQGILRVGGNKKGIFTRDRK
                     PKSAAFLLQKRWTGMNFGEKPQQGGKQ"
     protein_bind    complement(6047..6067)
                     /gene="mutant version of attB"
                     /label=attB3
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     primer_bind     complement(6267..6288)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     6479..6498
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     terminator      complement(6780..7032)
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     promoter        8992..9171
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     CDS             9213..9764
                     /codon_start=1
                     /gene="Streptomyces hygroscopicus bar"
                     /product="phosphinothricin acetyltransferase"
                     /label=BlpR
                     /note="confers resistance to bialophos or phosphinothricin"
                     /translation="MSPERRPADIRRATEADMPAVCTIVNHYIETSTVNFRTEPQEPQE
                     WTDDLVRLRERYPWLVAEVDGEVAGIAYAGPWKARNAYDWTAESTVYVSPRHQRTGLGS
                     TLYTHLLKSLEAQGFKSVVAVIGLPNDPSVRMHEALGYAPRGMLRAAGFKHGNWHDVGF
                     WQLDFSLPVPPRPVLPVTEI"
     terminator      9784..10036
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     misc_feature    10129..10153
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             10674..11465
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MGEAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      11711..12299
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     12200..12219
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     12453..12470
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    12485..12625
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(12551..12570)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     12711..12733
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     rep_origin      12969..13163
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     CDS             complement(13229..14302)
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
ORIGIN
        1 tgctcattta ctctggtagc tgcgcgatgt attcagatag cagctcggta atggtcttgc
       61 cttggcgtac cgcgtacatc ttcagcttgg tgtgatcctc cgccggcaac tgaaagttga
      121 cccgcttcat ggctggcgtg tctgccaggc tggccaacgt tgcagccttg ctgctgcgtg
      181 cgctcggacg gccggcactt agcgtgtttg tgcttttgct cattttctct ttacctcatt
      241 aactcaaatg agttttgatt taatttcagc ggccagcgcc tggacctcgc gggcagcgtc
      301 gccctcgggt tctgattcaa gaacggttgt gccggcggcg gcagtgcctg ggtagctcac
      361 gcgctgcgtg atacgggact caagaatggg cagctcgtac ccggccagcg cctcggcaac
      421 ctcaccgccg atgcgcgtgc ctttgatcgc ccgcgacacg acaaaggccg cttgtagcct
      481 tccatccgtg acctcaatgc gctgcttaac cagctccacc aggtcggcgg tggcccatat
      541 gtcgtaaggg cttggctgca ccggaatcag cacgaagtcg gctgccttga tcgcggacac
      601 agccaagtcc gccgcctggg gcgctccgtc gatcactacg aagtcgcgcc ggccgatggc
      661 cttcacgtcg cggtcaatcg tcgggcggtc gatgccgaca acggttagcg gttgatcttc
      721 ccgcacggcc gcccaatcgc gggcactgcc ctggggatcg gaatcgacta acagaacatc
      781 ggccccggcg agttgcaggg cgcgggctag atgggttgcg atggtcgtct tgcctgaccc
      841 gcctttctgg ttaagtacag cgataacctt catgcgttcc ccttgcgtat ttgtttattt
      901 actcatcgca tcatatacgc agcgaccgca tgacgcaagc tgttttactc aaatacacat
      961 caccttttta gacggcggcg ctcggtttct tcagcggcca agctggccgg ccaggccgcc
     1021 agcttggcat cagacaaacc ggccaggatt tcatgcagcc gcacggttga gacgtgcgcg
     1081 ggcggctcga acacgtaccc ggccgcgatc atctccgcct cgatctcttc ggtaatgaaa
     1141 aacggttcgt cctggccgtc ctggtgcggt ttcatgcttg ttcctcttgg cgttcattct
     1201 cggcggccgc cagggcgtcg gcctcggtca atgcgtcctc acggaaggca ccgcgccgcc
     1261 tggcctcggt gggcgtcact tcctcgctgc gctcaagtgc gcggtacagg gtcgagcgat
     1321 gcacgccaag cagtgcagcc gcctctttca cggtgcggcc ttcctggtcg atcagctcgc
     1381 gggcgtgcgc gatctgtgcc ggggtgaggg tagggcgggg gccaaacttc acgcctcggg
     1441 ccttggcggc ctcgcgcccg ctccgggtgc ggtcgatgat tagggaacgc tcgaactcgg
     1501 caatgccggc gaacacggtc aacaccatgc ggccggccgg cgtggtggtg tcggcccacg
     1561 gctctgccag gctacgcagg cccgcgccgg cctcctggat gcgctcggca atgtccagta
     1621 ggtcgcgggt gctgcgggcc aggcggtcta gcctggtcac tgtcacaacg tcgccagggc
     1681 gtaggtggtc aagcatcctg gccagctccg ggcggtcgcg cctggtgccg gtgatcttct
     1741 cggaaaacag cttggtgcag ccggccgcgt gcagttcggc ccgttggttg gtcaagtcct
     1801 ggtcgtcggt gctgacgcgg gcatagccca gcaggccagc ggcggcgctc ttgttcatgg
     1861 cgtaatgtct ccggttctag tcgcaagtat tctactttat gcgactaaaa cacgcgacaa
     1921 gaaaacgcca ggaaaagggc agggcggcag cctgtcgcgt aacttaggac ttgtgcgaca
     1981 tgtcgttttc agaagacggc tgcactgaac gtcagaagcc gactgcacta tagcagcgga
     2041 ggggttggat caaagtactt taaagtactt taaagtactt taaagtactt tgatcccgag
     2101 gggaaccctg tggttggcat gcacatacaa atggacgaac ggataaacct tttcacgccc
     2161 ttttaaatat ccgattattc taataaacgc tcttttctct taggtttacc cgccaatata
     2221 tcctgtcaaa cactgatagt ttaaactgaa ggcgggaaac gacaatctga tccaagctca
     2281 agctaagctt cacacaggaa acagctatga ccatgattac gccaagctat caactttgta
     2341 tagaaaagtt ggccagagac caagcttctc gagggtctct cgcgccaaag ggcgcgccta
     2401 acccgggtac cacaagtttg tacaaaaaag caggctccgc ggccgccccc ttggctaaga
     2461 atcaaggttg agaaaaagag aggattttta gggtttatga gcagctgaaa atgtaagaga
     2521 agaagaaagg gttttaagag attatttata agacagaggg ttttaatacg aagccgtttt
     2581 agggttttaa tacgacgccg ttagggtctt tactctttag ggtttttctt cagtactaac
     2641 gacgtcgtta aagatatgaa agtttgtgaa aaaacaaaat gctccgatca aattcaaacg
     2701 gcggaaatca gtataggcga aatcttcctc cgtcgaagct caggggaatg tcttcaagct
     2761 ctcaaggctt cacgattgat gcagccatgg agctgctgat ttcgacaaaa tgtggttaat
     2821 actctcgagc ggcgtttgtt ctatatccct tctttcaaaa tcaaccgtga cgtcgccgga
     2881 ctatttgatt aaggattgtg ctgttaaatc caatgttctt agcttctggc gtcaagtaag
     2941 tacggaacat cattcacctt tctcttttga gtagaaattg agacaaattt gacatgattc
     3001 atagagaaga gtagagattg ttttaggttt gttctcagat agcatttcat tcttaaggag
     3061 aatatgtatg aagtggattg tccatgtgtg acaccagagg ttgttctcaa ggcatctgga
     3121 catgtagacc agttcactga tctaatggtt aagaatgaga aaactggaac ttgttacagt
     3181 gcggacggac cacttgctta aggattattg ctccagacac gaagaacact ctaacaaggc
     3241 atttttgtga aggacttgta ttactacaat gggaggaaat ttccctttgc tgctgctcaa
     3301 attggtcaac ctttagaaat gaggtgatta tcgtttaatt ttctattcct ttgcttgata
     3361 gattggatgt gtttatctca atcagggact cttttttctt tcttttatgt ctggctttta
     3421 ataagtgacg catattactt gcagatatct catcgtcaag ggcttcttag agtttgtgaa
     3481 ttcacgctgg cagaaattga gcattttgtt gatcctggga ataagtcaca tccgaaattc
     3541 tctgatgtag caaattttga attccttatg tttccaagag aggaacaaat gtctggccaa
     3601 tctgcgaaaa aactttgcct tggcgaagtt gttgccaagg tcagtatctg taatcatctg
     3661 cgtctactta cttctagtat gcactccctc agaccttttg tttcttaatt gcttattgat
     3721 gcctgcaact gtatttgaat agggaactgt gaacaaagaa actgtaggct acttcattgc
     3781 gagagtgtat cttttccttg tccgtcttgg cacagacaag gaacagttgc gtttccgcca
     3841 gcattttgca aatgaaatgg cccgctatgc tgcagattgt ttggatgctg aatttgagag
     3901 ttcatatggg tggattgaat gtgttggtat agcagatagg tctgggtgaa gggtgggcgc
     3961 gccgacccag ctttcttgta caaagtgggg cgcgccatgt tacgtcctgt agaaacccca
     4021 acccgtgaaa tcaaaaaact cgacggcctg tgggcattca gtctggatcg cgaaaactgt
     4081 ggaattgatc agcgttggtg ggaaagcgcg ttacaagaaa gccgggcaat tgctgtgcca
     4141 ggcagtttta acgatcagtt cgccgatgca gattttcgta attatgcggg caacgtctgg
     4201 tatcagcgcg aagtctttat accgaaaggt tgggcaggcc agcgtatcgt gctgcgtttc
     4261 gatgcggtca ctcattacgg caaagtgtgg gtcaataatc aggaagtgat ggagcatcag
     4321 ggcggctata cgccatttga agccgatgtc acgccgtatg ttattgccgg gaaaagtgta
     4381 cgtatcaccg tttgcgtgaa caacgaactg aactggcaga ctatcccgcc gggaatggtg
     4441 attaccgacg aaaacggcaa gaaaaagcag tcttacttcc atgatttctt taactatgcc
     4501 ggaatccatc gcagcgtaat gctctacacc acgccgaaca cctgggtgga cgatatcacc
     4561 gtggtgacgc atgtcgcgca agactgtaac cacgcgtctg ttgactggca ggtggtggcc
     4621 aatggtgatg tcagcgttga actgcgtgat gcggatcaac aggtggttgc aactggacaa
     4681 ggcactagcg ggactttgca agtggtgaat ccgcacctct ggcaaccggg tgaaggttat
     4741 ctctatgaac tgtgcgtcac agccaaaagc cagacagagt gtgatatcta cccgcttcgc
     4801 gtcggcatcc ggtcagtggc agtgaagggc gaacagttcc tgattaacca caaaccgttc
     4861 tactttactg gctttggtcg tcatgaagat gcggacttgc gtggcaaagg attcgataac
     4921 gtgctgatgg tgcacgacca cgcattaatg gactggattg gggccaactc ctaccgtacc
     4981 tcgcattacc cttacgctga agagatgctc gactgggcag atgaacatgg catcgtggtg
     5041 attgatgaaa ctgctgctgt cggctttaac ctctctttag gcattggttt cgaagcgggc
     5101 aacaagccga aagaactgta cagcgaagag gcagtcaacg gggaaactca gcaagcgcac
     5161 ttacaggcga ttaaagagct gatagcgcgt gacaaaaacc acccaagcgt ggtgatgtgg
     5221 agtattgcca acgaaccgga tacccgtccg caaggtgcac gggaatattt cgcgccactg
     5281 gcggaagcaa cgcgtaaact cgacccgacg cgtccgatca cctgcgtcaa tgtaatgttc
     5341 tgcgacgctc acaccgatac catcagcgat ctctttgatg tgctgtgcct gaaccgttat
     5401 tacggatggt atgtccaaag cggcgatttg gaaacggcag agaaggtact ggaaaaagaa
     5461 cttctggcct ggcaggagaa actgcatcag ccgattatca tcaccgaata cggcgtggat
     5521 acgttagccg ggctgcactc aatgtacacc gacatgtgga gtgaagagta tcagtgtgca
     5581 tggctggata tgtatcaccg cgtctttgat cgcgtcagcg ccgtcgtcgg tgaacaggta
     5641 tggaatttcg ccgattttgc gacctcgcaa ggcatattgc gcgttggcgg taacaagaaa
     5701 gggatcttca ctcgcgaccg caaaccgaag tcggcggctt ttctgctgca aaaacgctgg
     5761 actggcatga acttcggtga aaaaccgcag cagggaggca aacaaggtac ctaaccgcgg
     5821 ccatgctaga gtccgcaaaa atcaccagtc tctctctaca aatctatctc tctctatttt
     5881 tctccagaat aatgtgtgag tagttcccag ataagggaat tagggttctt atagggtttc
     5941 gctcatgtgt tgagcatata agaaaccctt agtatgtatt tgtatttgta aaatacttct
     6001 atcaataaaa tttctaattc ctaaaaccaa aatccagtga tccggacaac tttattatac
     6061 atagttgata attcactggc cgtcgtgacg tcgggcccat cgatggcgcg ctgattgccc
     6121 ttcaccgcct ggccctgaga gagttgcagc aagcggtcca cgctggtttg ccccagcagg
     6181 cgaaaatcct gtttgatggt ggttccgaaa tcggcaaaat cccttataaa tcaaaagaat
     6241 agcccgagat agggttgagt gttgttccag tttggaacaa gagtccacta ttaaagaacg
     6301 tggactccaa cgtcaaaggg cgaaaaaccg tctatcaggg cgatggccca ctacgtgaac
     6361 catcacccaa atcaagtttt ttggggtcga ggtgccgtaa agcactaaat cggaacccta
     6421 aagggagccc ccgatttaga gcttgacggg gaaagccggc gaacgtggcg agaaaggaag
     6481 ggaagaaagc gaaaggagcg ggcgccattc aggccgatag cagcaccgta atcagtagcg
     6541 acagaatcaa gtttgccttt agcgtcagac tgtagcgcgt tttcatcggc attttcggtc
     6601 atagccccct tattagcgtt tgccatcttt tcataatcaa aatcaccgga accagagcca
     6661 ccaccggaac cgcctccctc agagccgcca ccctcagaac cgccaccctc agagccacca
     6721 ccctcagagc cgccaccaga accaccacca gagccgccgc cagcattgac aggaggcccg
     6781 atctagtaac atagatgaca ccgcgcgcga taatttatcc tagtttgcgc gctatatttt
     6841 gttttctatc gcgtattaaa tgtataattg cgggactcta atcataaaaa cccatctcat
     6901 aaataacgtc atgcattaca tgttaattat tacatgctta acgtaattca acagaaatta
     6961 tatgataatc atcgcaagac cggcaacagg attcaatctt aagaaacttt attgccaaat
     7021 gtttgaacga tcggggatcc gctttacttg tacataagga acagatggtg gcgtccctcg
     7081 gttctttcat actgctcaac gattgtatag tcttcgttgt ggcttgttat atccagtttg
     7141 gagtcaacat agtagtaccc tggtagctgc acaggcttct ttgccatgta aatacttttg
     7201 aattcaacta ggtaatgacc accgtctttc agcttcagag ccttatgaat ctctcctttc
     7261 aacacgccat cacgaggata caaacgctca gtgctggctt cccagcccat tgtcttcttt
     7321 tgcataacag gtccatcgga aggaaagttc acgccaatga acttgacctt gtagatgaaa
     7381 cagccatcct gcaaactgga atcctgggtt acagtaacga cgccaccgtc ttcaaagttc
     7441 atgacccttt cccatttaaa tccttcagga aatgacagct ttttatagtc tggtatgtcg
     7501 gcagggtgct tgacatatac cttgcttcca tactgaaatt gtggtgacaa aatatcccaa
     7561 gcaaatggca aaggtccccc cttggttacc ttaagcttta cggtattgtg gccttcgtat
     7621 ggcctcccct ctccttcgcc ttctatttca aactcgtgcc cattgaccgt tccttccatg
     7681 cgaaccttaa acctcatgaa ctccttgata acattcttgg aagaccccat ggggtatgtt
     7741 tttaatcttg tttgtattga tgagttttgg tttgagtaaa gagtgaagcg gatgagttaa
     7801 tttatagact ataaaggaga tttgcatggc gatcacgtgt aataatgcat gcacgcatgt
     7861 gattgtatgt gtgtgctgtg agagagaagc tcttaggtgt ttgaagggag tgacaagtgg
     7921 cgaagaaaaa caattctccg cggctgcatg ctatgtgtaa cgtgtagcta atgttctggc
     7981 atggcatctt atgaacgatt ctttttaaaa acaaggtaaa aacttaactt cataaaatta
     8041 aaaaaaagag cgtttactaa gttggtttaa aaggggatga gagtctataa attttggagg
     8101 tagtgccgtt gggaatataa attgggagct taatcagaat tatagaagtt aaagttgatt
     8161 tagtcacggt caatataaat tgggaatttg agtcaaaatc ttccaaattc ggaatccgtc
     8221 ttgttacacc cggtggatag gagccgaacg gtttgaaaat acttgaaatg tggatgcagg
     8281 tgcaggctgg tttaatttta tgttgaatgg atacatgtca atcgaatttg agttataggt
     8341 acacatttta ctctgatact aaaatgtaac atttgtctca agaatgggta ggtcatcctt
     8401 actcgacaac ggtatgccac ttcacttgaa cgtatatctc attcaaccca actctaacac
     8461 acatttcata cccatgtcta aaataaagaa aattgaagaa aaacgctcaa tagcgtcaat
     8521 ccttacaaat ataatgtaca tggcaacctc gcatgaaaat agtaataaag aggagtgaat
     8581 aatataaagt agtcactaat tggatgacaa agcaaatgga accatccgat agatgttttg
     8641 agaacaagag gtacatttgt atatatgcat tggttgatac atttctgcca tctgaattgt
     8701 ttcttcctca aagaagaagc attcagccaa tagtgaaatt taaaatgttg gaaaaatgag
     8761 gatgcagtca gaaagtccag aatataagtg gtaactggta actgaatact tgcctcagaa
     8821 gcaagataag aaacataagt ctttaaagga atcaatcacc gatgaagaga gctccgaatc
     8881 gaattcgagc tcggtaccgc gaattatcga tcatgagcgg agaattaagg gagtcacgtt
     8941 atgacccccg ccgatgacgc gggacaagcc gttttacgtt tggaactgac agaaccgcaa
     9001 cgttgaagga gccactgagc cgcgggtttc tggagtttaa tgagctaagc acatacgtca
     9061 gaaaccatta ttgcgcgttc aaaagtcgcc taaggtcact atcagctagc aaatatttct
     9121 tgtcaaaaat gctccactga cgttccataa attcccctcg gtatccaatt agagtctcat
     9181 attcactctc aactcgatcg aggggatcta ccatgagccc agaacgacgc ccggccgaca
     9241 tccgccgtgc caccgaggcg gacatgccgg cggtctgcac catcgtcaac cactacatcg
     9301 agacaagcac ggtcaacttc cgtaccgagc cgcaggaacc gcaggagtgg acggacgacc
     9361 tcgtccgtct gcgggagcgc tatccctggc tcgtcgccga ggtggacggc gaggtcgccg
     9421 gcatcgccta cgcgggtccc tggaaggcac gcaacgccta cgactggacg gccgagtcga
     9481 ccgtgtacgt ctccccccgc caccagcgga cgggactggg ctccacgctc tacacccacc
     9541 tgctgaagtc cctggaggca cagggcttca agagcgtggt cgctgtcatc gggctgccca
     9601 acgacccgag cgtgcgcatg cacgaggcgc tcggatatgc cccccgcggc atgctgcggg
     9661 cggccggctt caagcacggg aactggcatg acgtgggttt ctggcagctg gacttcagcc
     9721 tgccggtgcc gccccgtccg gtcctgcccg tcaccgaaat ctgatgaccc ctagagtcaa
     9781 gcagatcgtt caaacatttg gcaataaagt ttcttaagat tgaatcctgt tgccggtctt
     9841 gcgatgatta tcatataatt tctgttgaat tacgttaagc atgtaataat taacatgtaa
     9901 tgcatgacgt tatttatgag atgggttttt atgattagag tcccgcaatt atacatttaa
     9961 tacgcgatag aaaacaaaat atagcgcgca aactaggata aattatcgcg cgcggtgtca
    10021 tctatgttac tagatcgacc ggcatgcaag ctgataattc aattcggcgt taattcagta
    10081 cattaaaaac gtccgcaatg tgttattaag ttgtctaagc gtcaatttgt ttacaccaca
    10141 atatatcctg ccaccagcca gccaacagct ccccgaccgg cagctcggca caaaatcacc
    10201 actcgataca ggcagcccat cagtccggga cggcgtcagc gggagagccg ttgtaaggcg
    10261 gcagactttg ctcatgttac cgatgctatt cggaagaacg gcaactaagc tgccgggttt
    10321 gaaacacgga tgatctcgcg gagggtagca tgttgattgt aacgatgaca gagcgttgct
    10381 gcctgtgatc aattcgggca cgaacccagt ggacataagc ctgttcggtt cgtaagctgt
    10441 aatgcaagta gcgtatgcgc tcacgcaact ggtccagaac cttgaccgaa cgcagcggtg
    10501 gtaacggcgc agtggcggtt ttcatggctt gttatgactg tttttttggg gtacagtcta
    10561 tgcctcgggc atccaagcag caagcgcgtt acgccgtggg tcgatgtttg atgttatgga
    10621 gcagcaacga tgttacgcag cagggcagtc gccctaaaac aaagttaaac atcatggggg
    10681 aagcggtgat cgccgaagta tcgactcaac tatcagaggt agttggcgtc atcgagcgcc
    10741 atctcgaacc gacgttgctg gccgtacatt tgtacggctc cgcagtggat ggcggcctga
    10801 agccacacag tgatattgat ttgctggtta cggtgaccgt aaggcttgat gaaacaacgc
    10861 ggcgagcttt gatcaacgac cttttggaaa cttcggcttc ccctggagag agcgagattc
    10921 tccgcgctgt agaagtcacc attgttgtgc acgacgacat cattccgtgg cgttatccag
    10981 ctaagcgcga actgcaattt ggagaatggc agcgcaatga cattcttgca ggtatcttcg
    11041 agccagccac gatcgacatt gatctggcta tcttgctgac aaaagcaaga gaacatagcg
    11101 ttgccttggt aggtccagcg gcggaggaac tctttgatcc ggttcctgaa caggatctat
    11161 ttgaggcgct aaatgaaacc ttaacgctat ggaactcgcc gcccgactgg gctggcgatg
    11221 agcgaaatgt agtgcttacg ttgtcccgca tttggtacag cgcagtaacc ggcaaaatcg
    11281 cgccgaagga tgtcgctgcc gactgggcaa tggagcgcct gccggcccag tatcagcccg
    11341 tcatacttga agctagacag gcttatcttg gacaagaaga agatcgcttg gcctcgcgcg
    11401 cagatcagtt ggaagaattt gtccactacg tgaaaggcga gatcaccaag gtagtcggca
    11461 aataatgtct agctagaaat tcgttcaagc cgacgccgct tcgcggcgcg gcttaactca
    11521 agcgttagat gcactaagca cataattgct cacagccaaa ctatcaggtc aagtctgctt
    11581 ttattatttt taagcgtgca taataagccc tacacaaatt gggagatata tcatgcatga
    11641 ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca
    11701 aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac
    11761 caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg
    11821 taactggctt cagcagagcg cagataccaa atactgtcct tctagtgtag ccgtagttag
    11881 gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac
    11941 cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt
    12001 taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg
    12061 agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc
    12121 ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc
    12181 gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc
    12241 acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa
    12301 acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt
    12361 tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg
    12421 ataccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag
    12481 agcgcctgat gcggtatttt ctccttacgc atctgtgcgg tatttcacac cgcatatggt
    12541 gcactctcag tacaatctgc tctgatgccg catagttaag ccagtataca ctccgctatc
    12601 gctacgtgac tgggtcatgg ctgcgccccg acacccgcca acacccgctg acgcgccctg
    12661 acgggcttgt ctgctcccgg catccgctta cagacaagct gtgaccgtct ccgggagctg
    12721 catgtgtcag aggttttcac cgtcatcacc gaaacgcgcg aggcagggtg ccttgatgtg
    12781 ggcgccggcg gtcgagtggc gacggcgcgg cttgtccgcg ccctggtaga ttgcctggcc
    12841 gtaggccagc catttttgag cggccagcgg ccgcgatagg ccgacgcgaa gcggcggggc
    12901 gtagggagcg cagcgaccga agggtaggcg ctttttgcag ctcttcggct gtgcgctggc
    12961 cagacagtta tgcacaggcc aggcgggttt taagagtttt aataagtttt aaagagtttt
    13021 aggcggaaaa atcgcctttt ttctctttta tatcagtcac ttacatgtgt gaccggttcc
    13081 caatgtacgg ctttgggttc ccaatgtacg ggttccggtt cccaatgtac ggctttgggt
    13141 tcccaatgta cgtgctatcc acaggaaaga gaccttttcg acctttttcc cctgctaggg
    13201 caatttgccc tagcatctgc tccgtacatt aggaaccggc ggatgcttcg ccctcgatca
    13261 ggttgcggta gcgcatgact aggatcgggc cagcctgccc cgcctcctcc ttcaaatcgt
    13321 actccggcag gtcatttgac ccgatcagct tgcgcacggt gaaacagaac ttcttgaact
    13381 ctccggcgct gccactgcgt tcgtagatcg tcttgaacaa ccatctggct tctgccttgc
    13441 ctgcggcgcg gcgtgccagg cggtagagaa aacggccgat gccgggatcg atcaaaaagt
    13501 aatcggggtg aaccgtcagc acgtccgggt tcttgccttc tgtgatctcg cggtacatcc
    13561 aatcagctag ctcgatctcg atgtactccg gccgcccggt ttcgctcttt acgatcttgt
    13621 agcggctaat caaggcttca ccctcggata ccgtcaccag gcggccgttc ttggccttct
    13681 tcgtacgctg catggcaacg tgcgtggtgt ttaaccgaat gcaggtttct accaggtcgt
    13741 ctttctgctt tccgccatcg gctcgccggc agaacttgag tacgtccgca acgtgtggac
    13801 ggaacacgcg gccgggcttg tctcccttcc cttcccggta tcggttcatg gattcggtta
    13861 gatgggaaac cgccatcagt accaggtcgt aatcccacac actggccatg ccggccggcc
    13921 ctgcggaaac ctctacgtgc ccgtctggaa gctcgtagcg gatcacctcg ccagctcgtc
    13981 ggtcacgctt cgacagacgg aaaacggcca cgtccatgat gctgcgacta tcgcgggtgc
    14041 ccacgtcata gagcatcgga acgaaaaaat ctggttgctc gtcgcccttg ggcggcttcc
    14101 taatcgacgg cgcaccggct gccggcggtt gccgggattc tttgcggatt cgatcagcgg
    14161 ccgcttgcca cgattcaccg gggcgtgctt ctgcctcgat gcgttgccgc tgggcggcct
    14221 gcgcggcctt caacttctcc accaggtcat cacccagcgc cgcgccgatt tgtaccgggc
    14281 cggatggttt gcgaccgctc acgccgattc ctcgggcttg ggggttccag tgccattgca
    14341 gggccggcag acaacccagc cgcttacgcc tggccaaccg cccgttcctc cacacatggg
    14401 gcattccacg gcgtcggtgc ctggttgttc ttgattttcc atgccgcctc ctttagccgc
    14461 taaaattcat ctactcattt attcatt
//
