LOCUS       sequence-207997-       14740 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Arabidopsis transformation, expresses GA2ox2 promoter/GUS fusion to 
            study expression pattern in roots.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 14740)
  AUTHORS   Li H, Torres-Garcia J, Latrasse D, Benhamed M, Schilderink S, Zhou 
            W, Kulikova O, Hirt H, Bisseling T
  TITLE     Plant-Specific Histone Deacetylases HDT1/2 Regulate GIBBERELLIN 
            2-OXIDASE2 Expression to Control Arabidopsis Root Meristem Cell 
            Number.
  JOURNAL   Plant Cell. 2017 Sep;29(9):2183-2196. doi: 10.1105/tpc.17.00366. 
            Epub 2017 Aug 30.
  PUBMED    28855334
REFERENCE   2  (bases 1 to 14740)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1105/tpc.17.00366"; journalName: "Plant Cell"; date: "2017-09"; 
            volume: "29"; issue: "9"; pages: "2183-2196"
COMMENT     Sequence Label: pGA2ox2:GUS
FEATURES             Location/Qualifiers
     source          1..14740
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    1693..1717
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     primer_bind     1784..1800
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     1784..1800
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     protein_bind    1820..1840
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     protein_bind    3637..3661
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             3691..5499
                     /codon_start=1
                     /gene="uidA (or gusA) from E. coli"
                     /product="beta-glucuronidase"
                     /label=GUS
                     /translation="MLRPVETPTREIKKLDGLWAFSLDRENCGIDQRWWESALQESRAI
                     AVPGSFNDQFADADFRNYAGNVWYQREVFIPKGWAGQRIVLRFDAVTHYGKVWVNNQEV
                     MEHQGGYTPFEADVTPYVIAGKSVRITVCVNNELNWQTIPPGMVITDENGKKKQSYFHD
                     FFNYAGIHRSVMLYTTPNTWVDDITVVTHVAQDCNHASVDWQVVANGDVSVELRDADQQ
                     VVATGQGTSGTLQVVNPHLWQPGEGYLYELCVTAKSQTECDIYPLRVGIRSVAVKGEQF
                     LINHKPFYFTGFGRHEDADLRGKGFDNVLMVHDHALMDWIGANSYRTSHYPYAEEMLDW
                     ADEHGIVVIDETAAVGFNLSLGIGFEAGNKPKELYSEEAVNGETQQAHLQAIKELIARD
                     KNHPSVVMWSIANEPDTRPQGAREYFAPLAEATRKLDPTRPITCVNVMFCDAHTDTISD
                     LFDVLCLNRYYGWYVQSGDLETAEKVLEKELLAWQEKLHQPIIITEYGVDTLAGLHSMY
                     TDMWSEEYQCAWLDMYHRVFDRVSAVVGEQVWNFADFATSQGILRVGGNKKGIFTRDRK
                     PKSAAFLLQKRWTGMNFGEKPQQGGKQ"
     protein_bind    complement(5525..5549)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(5810..5830)
                     /gene="mutant version of attB"
                     /label=attB3
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     CDS             join(7359..7361,7362..7364,7365..8039)
                     /codon_start=1
                     /product="wild-type DsRed"
                     /label=DsRed1
                     /note="mammalian codon-optimized"
                     /translation="MARSSKNVIKEFMRFKVRMEGTVNGHEFEIEGEGEGRPYEGHNTV
                     KLKVTKGGPLPFAWDILSPQFQYGSKVYVKHPADIPDYKKLSFPEGFKWERVMNFEDGG
                     VVTVTQDSSLQDGCFIYKVKFIGVNFPSDGPVMQKKTMGWEASTERLYPRDGVLKGEIH
                     KALKLKDGGHYLVEFKSIYMAKKPVQLPGYYYVDSKLDITSHNEDYTIVEQYERTEGRH
                     HLFL"
     primer_bind     complement(7542..7562)
                     /label=DsRed1-N
                     /note="DsRed1, reverse primer"
     primer_bind     7760..7779
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     7954..7977
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
     terminator      8052..8304
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     promoter        8479..8658
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     CDS             8692..9486
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(8746..8765)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     9356..9375
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     terminator      9526..9778
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     misc_feature    9871..9895
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             10416..11207
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MGEAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      11453..12041
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     11942..11961
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     12195..12212
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    12227..12367
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(12293..12312)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     12453..12475
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     rep_origin      12711..12905
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     CDS             complement(12971..14044)
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     CDS             complement(join(14473..14740,1..362))
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
ORIGIN
        1 agctccacca ggtcggcggt ggcccatatg tcgtaagggc ttggctgcac cggaatcagc
       61 acgaagtcgg ctgccttgat cgcggacaca gccaagtccg ccgcctgggg cgctccgtcg
      121 atcactacga agtcgcgccg gccgatggcc ttcacgtcgc ggtcaatcgt cgggcggtcg
      181 atgccgacaa cggttagcgg ttgatcttcc cgcacggccg cccaatcgcg ggcactgccc
      241 tggggatcgg aatcgactaa cagaacatcg gccccggcga gttgcagggc gcgggctaga
      301 tgggttgcga tggtcgtctt gcctgacccg cctttctggt taagtacagc gataaccttc
      361 atgcgttccc cttgcgtatt tgtttattta ctcatcgcat catatacgca gcgaccgcat
      421 gacgcaagct gttttactca aatacacatc acctttttag acggcggcgc tcggtttctt
      481 cagcggccaa gctggccggc caggccgcca gcttggcatc agacaaaccg gccaggattt
      541 catgcagccg cacggttgag acgtgcgcgg gcggctcgaa cacgtacccg gccgcgatca
      601 tctccgcctc gatctcttcg gtaatgaaaa acggttcgtc ctggccgtcc tggtgcggtt
      661 tcatgcttgt tcctcttggc gttcattctc ggcggccgcc agggcgtcgg cctcggtcaa
      721 tgcgtcctca cggaaggcac cgcgccgcct ggcctcggtg ggcgtcactt cctcgctgcg
      781 ctcaagtgcg cggtacaggg tcgagcgatg cacgccaagc agtgcagccg cctctttcac
      841 ggtgcggcct tcctggtcga tcagctcgcg ggcgtgcgcg atctgtgccg gggtgagggt
      901 agggcggggg ccaaacttca cgcctcgggc cttggcggcc tcgcgcccgc tccgggtgcg
      961 gtcgatgatt agggaacgct cgaactcggc aatgccggcg aacacggtca acaccatgcg
     1021 gccggccggc gtggtggtgt cggcccacgg ctctgccagg ctacgcaggc ccgcgccggc
     1081 ctcctggatg cgctcggcaa tgtccagtag gtcgcgggtg ctgcgggcca ggcggtctag
     1141 cctggtcact gtcacaacgt cgccagggcg taggtggtca agcatcctgg ccagctccgg
     1201 gcggtcgcgc ctggtgccgg tgatcttctc ggaaaacagc ttggtgcagc cggccgcgtg
     1261 cagttcggcc cgttggttgg tcaagtcctg gtcgtcggtg ctgacgcggg catagcccag
     1321 caggccagcg gcggcgctct tgttcatggc gtaatgtctc cggttctagt cgcaagtatt
     1381 ctactttatg cgactaaaac acgcgacaag aaaacgccag gaaaagggca gggcggcagc
     1441 ctgtcgcgta acttaggact tgtgcgacat gtcgttttca gaagacggct gcactgaacg
     1501 tcagaagccg actgcactat agcagcggag gggttggatc aaagtacttt aaagtacttt
     1561 aaagtacttt aaagtacttt gatcccgagg ggaaccctgt ggttggcatg cacatacaaa
     1621 tggacgaacg gataaacctt ttcacgccct tttaaatatc cgattattct aataaacgct
     1681 cttttctctt aggtttaccc gccaatatat cctgtcaaac actgatagtt taaactgaag
     1741 gcgggaaacg acaatctgat ccaagctcaa gctaagcttc acacaggaaa cagctatgac
     1801 catgattacg ccaagctatc aactttgtat agaaaagttg caaccgaaca agtctaactg
     1861 aaccaaacca aaacttgaag atgcgtatcc tacatttgaa tattatataa gagaaattgg
     1921 tggaaaatta tacgaaattt aaatttattg taagtactat tattatttgg taatcggaag
     1981 ttctgatcca taggcatcat gtaaatacta caatagttaa atacgaccac atcaaaacca
     2041 aatcctagga aacacatgag gttatgattt taaccaccat actcttactg gtaataattc
     2101 accaccgaga aaccggagag aacctacaac cagaaagcac ctacatcgaa ttttggaagc
     2161 aataaagatt acattatgta tcagaaataa tcgttaatac atgtaacaat gaattatttg
     2221 tttcgagtgc aaggtttatt tatcatgatt cacagtatat ctaaaataat aatttttttg
     2281 tttagatata ttaagagctc cctctggcag tttgatttaa acctagaatt tgtaatttgt
     2341 atacttctga tttcaaattc atccctaaaa cggtaaaaat atatatttca acccgaatgt
     2401 tcaaaataaa attatatatg tatatatatt cccaacccta catgcactta gatattattg
     2461 aacaaataaa ttaaatagag taaacaaaag ttaattactg cgcgtcggag agaagccttg
     2521 gcgtccaagt cttaccaccg aaaatatgtc gacttcccat ttgctttaac ttaatttgat
     2581 aatattataa atatactaac attttactga ctcgaataca ataatagtga ttccagtaaa
     2641 ttattaacta ataaagaatg gcaatgcttt ttttttttta agaatgacaa tatttcataa
     2701 gattggcaag atgcaattgt tttttctatc gtgtgaatct gagaatctca ctcaccgccc
     2761 aatctataac cctgttcaag tttaactatt tctagtagca cgttagaaaa gagctaaata
     2821 tagaagactt tatcgatggg ttttatcttc aacaatatct acgtaggaat atatttcctg
     2881 taaaagcatt tagtcacgtt ccttattatt aatatctttt taatttcagt gacctaaata
     2941 ttagattgtg ggcttgacaa tatccctaat actaaatagt agtgttttct ctacatatat
     3001 tttgtatttt cattttaacg aaaaaagaaa tttcatcaaa agactatagc tttttcttac
     3061 ataaagcata actttatctt gtacattata atcgtgaaat tgttgaaaca tttcgagtca
     3121 agaattaatt aagtaaaact acgacccgtg attcttttct tcctttgtcg acaatccata
     3181 cgcgtgaata cgtgattagt caaatgtaaa atgcaaaaag aaaaaacata aaacaggacc
     3241 ataccaatac catattctca ctataccaac ttcgatttca taaacccaaa taagaaaata
     3301 aaaacaaaaa ctccacgctg tatttccatg tgtgcgagat acaagaatag actcgacatg
     3361 caaaataaaa ataaatatga ccaaataatc atataatatc ttagaataaa taaattaaaa
     3421 aatcatagta accattttat aaatttcgta accttttctc cgtcatcccc cacataccac
     3481 tccctataaa tgccaagcaa aatagctttc ctcgatagtt cctaaatcat aactacaaaa
     3541 acttttcctt cttcttcctc aacctttgct tcaatcttca acaactttct ttttataaag
     3601 attttgcaag ttaagtgtaa acctacaaaa accaaaccaa gtttgtacaa aaaagcaggc
     3661 tccgcggccg cccccttcac cgggcgcgcc atgttacgtc ctgtagaaac cccaacccgt
     3721 gaaatcaaaa aactcgacgg cctgtgggca ttcagtctgg atcgcgaaaa ctgtggaatt
     3781 gatcagcgtt ggtgggaaag cgcgttacaa gaaagccggg caattgctgt gccaggcagt
     3841 tttaacgatc agttcgccga tgcagatttt cgtaattatg cgggcaacgt ctggtatcag
     3901 cgcgaagtct ttataccgaa aggttgggca ggccagcgta tcgtgctgcg tttcgatgcg
     3961 gtcactcatt acggcaaagt gtgggtcaat aatcaggaag tgatggagca tcagggcggc
     4021 tatacgccat ttgaagccga tgtcacgccg tatgttattg ccgggaaaag tgtacgtatc
     4081 accgtttgcg tgaacaacga actgaactgg cagactatcc cgccgggaat ggtgattacc
     4141 gacgaaaacg gcaagaaaaa gcagtcttac ttccatgatt tctttaacta tgccggaatc
     4201 catcgcagcg taatgctcta caccacgccg aacacctggg tggacgatat caccgtggtg
     4261 acgcatgtcg cgcaagactg taaccacgcg tctgttgact ggcaggtggt ggccaatggt
     4321 gatgtcagcg ttgaactgcg tgatgcggat caacaggtgg ttgcaactgg acaaggcact
     4381 agcgggactt tgcaagtggt gaatccgcac ctctggcaac cgggtgaagg ttatctctat
     4441 gaactgtgcg tcacagccaa aagccagaca gagtgtgata tctacccgct tcgcgtcggc
     4501 atccggtcag tggcagtgaa gggcgaacag ttcctgatta accacaaacc gttctacttt
     4561 actggctttg gtcgtcatga agatgcggac ttgcgtggca aaggattcga taacgtgctg
     4621 atggtgcacg accacgcatt aatggactgg attggggcca actcctaccg tacctcgcat
     4681 tacccttacg ctgaagagat gctcgactgg gcagatgaac atggcatcgt ggtgattgat
     4741 gaaactgctg ctgtcggctt taacctctct ttaggcattg gtttcgaagc gggcaacaag
     4801 ccgaaagaac tgtacagcga agaggcagtc aacggggaaa ctcagcaagc gcacttacag
     4861 gcgattaaag agctgatagc gcgtgacaaa aaccacccaa gcgtggtgat gtggagtatt
     4921 gccaacgaac cggatacccg tccgcaaggt gcacgggaat atttcgcgcc actggcggaa
     4981 gcaacgcgta aactcgaccc gacgcgtccg atcacctgcg tcaatgtaat gttctgcgac
     5041 gctcacaccg ataccatcag cgatctcttt gatgtgctgt gcctgaaccg ttattacgga
     5101 tggtatgtcc aaagcggcga tttggaaacg gcagagaagg tactggaaaa agaacttctg
     5161 gcctggcagg agaaactgca tcagccgatt atcatcaccg aatacggcgt ggatacgtta
     5221 gccgggctgc actcaatgta caccgacatg tggagtgaag agtatcagtg tgcatggctg
     5281 gatatgtatc accgcgtctt tgatcgcgtc agcgccgtcg tcggtgaaca ggtatggaat
     5341 ttcgccgatt ttgcgacctc gcaaggcata ttgcgcgttg gcggtaacaa gaaagggatc
     5401 ttcactcgcg accgcaaacc gaagtcggcg gcttttctgc tgcaaaaacg ctggactggc
     5461 atgaacttcg gtgaaaaacc gcagcaggga ggcaaacaag gtaccaagaa gggtgggcgc
     5521 gccgacccag ctttcttgta caaagtgggg cgcgcctaac ccgggaatgg tacctaaccg
     5581 cggccatgct agagtccgca aaaatcacca gtctctctct acaaatctat ctctctctat
     5641 ttttctccag aataatgtgt gagtagttcc cagataaggg aattagggtt cttatagggt
     5701 ttcgctcatg tgttgagcat ataagaaacc cttagtatgt atttgtattt gtaaaatact
     5761 tctatcaata aaatttctaa ttcctaaaac caaaatccag tgatccggac aactttatta
     5821 tacatagttg ataattcact ggccgtcgtg acgtcgttgt ggttggtgct ttccttacat
     5881 tctgagcctc tttccttcta atccactcat ctgcatcttc ttgtgtcctt actaatacct
     5941 cattggttcc aaattccctc cctttaagca ccagctcgtt tctgttcttc cacagcctcc
     6001 caagtatcca agggactaaa gcctccacat tcttcagatc aggatattct tgtttaagat
     6061 gttgaactct atggaggttt gtatgaactg atgatctagg accggataag ttcccttctt
     6121 catagcgaac ttattcaaag aatgttttgt gtatcattct tgttacattg ttattaatga
     6181 aaaaatatta ttggtcattg gactgaacac gagtgttaaa tatggaccag gccccaaata
     6241 agatccattg atatatgaat taaataacaa gaataaatcg agtcaccaaa ccacttgcct
     6301 tttttaacga gacttgttca ccaacttgat acaaaagtca ttatcctatg caaatcaata
     6361 atcatacaaa aatatccaat aacactaaaa aattaaaaga aatggataat ttcacaatat
     6421 gttatacgat aaagaagtta cttttccaag aaattcactg attttataag cccacttgca
     6481 ttagataaat ggcaaaaaaa aacaaaaagg aaaagaaata aagcacgaag aattctagaa
     6541 gatacgaaat acgcttcaat gcagtgggac ccacggttca attattgcca attttcagct
     6601 ccaccgtata tttaaaaaat aaaacgataa tgctaaaaaa atataaatcg taacgatcgt
     6661 taaatctcaa cggctggatc ttatgacgac cgttagaaat tgtggttgtc gacgagtcag
     6721 taataaacgg cgtcaaagtg gttgcagccg gcacacacga gtcgtgttta tcaactcaaa
     6781 gcacaaatac ttttcctcaa cctaaaaata aggcaattag ccaaaaacaa ctttgcgtgt
     6841 aaacaacgct caatacacgt gtcattttat tattagctat tgcttcaccg ccttagcttt
     6901 ctcgtgacct agtcgtcctc gtcttttctt cttcttcttc tataaaacaa tacccaaaga
     6961 gctcttcttc ttcacaattc agatttcaat ttctcaaaat cttaaaaact ttctctcaat
     7021 tctctctacc gtgatcaagg taaatttctg tgttccttat tctctcaaaa tcttcgattt
     7081 tgttttcgtt cgatcccaat ttcgtatatg ttctttggtt tagattctgt taatcttaga
     7141 tcgaagacga ttttctgggt ttgatcgtta gatatcatct taattctcga ttagggtttc
     7201 atagatatca tccgatttgt tcaaataatt tgagttttgt cgaataatta ctcttcgatt
     7261 tgtgatttct atctagatct ggtgttagtt tctagtttgt gcgatcgaat ttgtcgatta
     7321 atctgagttt ttctgattaa cagatgcaga tctttgccat ggcgcgctcc tccaagaacg
     7381 tcatcaagga gttcatgcgc ttcaaggtgc gcatggaggg caccgtgaac ggccacgagt
     7441 tcgagatcga gggcgagggc gagggccgcc cctacgaggg ccacaacacc gtgaagctga
     7501 aggtgaccaa gggcggcccc ctgcccttcg cctgggacat cctgtccccc cagttccagt
     7561 acggctccaa ggtgtacgtg aagcaccccg ccgacatccc cgactacaag aagctgtcct
     7621 tccccgaggg cttcaagtgg gagcgcgtga tgaacttcga ggacggcggc gtggtgaccg
     7681 tgacccagga ctcctccctg caggacggct gcttcatcta caaggtgaag ttcatcggcg
     7741 tgaacttccc ctccgacggc cccgtaatgc agaagaagac catgggctgg gaggcctcca
     7801 ccgagcgcct gtacccccgc gacggcgtgc tgaagggcga gatccacaag gccctgaagc
     7861 tgaaggacgg cggccactac ctggtggagt tcaagtccat ctacatggcc aagaagcccg
     7921 tgcagctgcc cggctactac tacgtggact ccaagctgga catcacctcc cacaacgagg
     7981 actacaccat cgtggagcag tacgagcgca ccgagggccg ccaccacctg ttcctgtagg
     8041 atccaattcc cgatcgttca aacatttggc aataaagttt cttaagattg aatcctgttg
     8101 ccggtcttgc gatgattatc atataatttc tgttgaatta cgttaagcat gtaataatta
     8161 acatgtaatg catgacgtta tttatgagat gggtttttat gattagagtc ccgcaattat
     8221 acatttaata cgcgatagaa aacaaaatat agcgcgcaaa ctaggataaa ttatcgcgcg
     8281 cggtgtcatc tatgttacta gatcgggggg cccaagggcg aattctgcag atatccatca
     8341 cactggcggc cgctcgagca tgcatctaga ggatccccgg gtaccgcgaa ttatcgatca
     8401 tgagcggaga attaagggag tcacgttatg acccccgccg atgacgcggg acaagccgtt
     8461 ttacgtttgg aactgacaga accgcaacgt tgaaggagcc actgagccgc gggtttctgg
     8521 agtttaatga gctaagcaca tacgtcagaa accattattg cgcgttcaaa agtcgcctaa
     8581 ggtcactatc agctagcaaa tatttcttgt caaaaatgct ccactgacgt tccataaatt
     8641 cccctcggta tccaattaga gtctcatatt cactctcaac tcgatcgagg catgattgaa
     8701 caagatggat tgcacgcagg ttctccggcc gcttgggtgg agaggctatt cggctatgac
     8761 tgggcacaac agacaatcgg ctgctctgat gccgccgtgt tccggctgtc agcgcagggg
     8821 cgcccggttc tttttgtcaa gaccgacctg tccggtgccc tgaatgaact ccaagacgag
     8881 gcagcgcggc tatcgtggct ggccacgacg ggcgttcctt gcgcagctgt gctcgacgtt
     8941 gtcactgaag cgggaaggga ctggctgcta ttgggcgaag tgccggggca ggatctcctg
     9001 tcatctcacc ttgctcctgc cgagaaagta tccatcatgg ctgatgcaat gcggcggctg
     9061 catacgcttg atccggctac ctgcccattc gaccaccaag cgaaacatcg catcgagcga
     9121 gcacgtactc ggatggaagc cggtcttgtc gatcaggatg atctggacga agagcatcag
     9181 gggctcgcgc cagccgaact gttcgccagg ctcaaggcgc ggatgcccga cggcgaggat
     9241 ctcgtcgtga cccacggcga tgcctgcttg ccgaatatca tggtggaaaa tggccgcttt
     9301 tctggattca tcgactgtgg ccggctgggt gtggcggacc gctatcagga catagcgttg
     9361 gctacccgtg atattgctga agagcttggc ggcgaatggg ctgaccgctt cctcgtgctt
     9421 tacggtatcg ccgctcccga ttcgcagcgc atcgccttct atcgccttct tgacgagttc
     9481 ttctgagcgg gactctgggg ttcggactct agctagagtc aagcagatcg ttcaaacatt
     9541 tggcaataaa gtttcttaag attgaatcct gttgccggtc ttgcgatgat tatcatataa
     9601 tttctgttga attacgttaa gcatgtaata attaacatgt aatgcatgac gttatttatg
     9661 agatgggttt ttatgattag agtcccgcaa ttatacattt aatacgcgat agaaaacaaa
     9721 atatagcgcg caaactagga taaattatcg cgcgcggtgt catctatgtt actagatcga
     9781 ccggcatgca agctgataat tcaattcggc gttaattcag tacattaaaa acgtccgcaa
     9841 tgtgttatta agttgtctaa gcgtcaattt gtttacacca caatatatcc tgccaccagc
     9901 cagccaacag ctccccgacc ggcagctcgg cacaaaatca ccactcgata caggcagccc
     9961 atcagtccgg gacggcgtca gcgggagagc cgttgtaagg cggcagactt tgctcatgtt
    10021 accgatgcta ttcggaagaa cggcaactaa gctgccgggt ttgaaacacg gatgatctcg
    10081 cggagggtag catgttgatt gtaacgatga cagagcgttg ctgcctgtga tcaattcggg
    10141 cacgaaccca gtggacataa gcctgttcgg ttcgtaagct gtaatgcaag tagcgtatgc
    10201 gctcacgcaa ctggtccaga accttgaccg aacgcagcgg tggtaacggc gcagtggcgg
    10261 ttttcatggc ttgttatgac tgtttttttg gggtacagtc tatgcctcgg gcatccaagc
    10321 agcaagcgcg ttacgccgtg ggtcgatgtt tgatgttatg gagcagcaac gatgttacgc
    10381 agcagggcag tcgccctaaa acaaagttaa acatcatggg ggaagcggtg atcgccgaag
    10441 tatcgactca actatcagag gtagttggcg tcatcgagcg ccatctcgaa ccgacgttgc
    10501 tggccgtaca tttgtacggc tccgcagtgg atggcggcct gaagccacac agtgatattg
    10561 atttgctggt tacggtgacc gtaaggcttg atgaaacaac gcggcgagct ttgatcaacg
    10621 accttttgga aacttcggct tcccctggag agagcgagat tctccgcgct gtagaagtca
    10681 ccattgttgt gcacgacgac atcattccgt ggcgttatcc agctaagcgc gaactgcaat
    10741 ttggagaatg gcagcgcaat gacattcttg caggtatctt cgagccagcc acgatcgaca
    10801 ttgatctggc tatcttgctg acaaaagcaa gagaacatag cgttgccttg gtaggtccag
    10861 cggcggagga actctttgat ccggttcctg aacaggatct atttgaggcg ctaaatgaaa
    10921 ccttaacgct atggaactcg ccgcccgact gggctggcga tgagcgaaat gtagtgctta
    10981 cgttgtcccg catttggtac agcgcagtaa ccggcaaaat cgcgccgaag gatgtcgctg
    11041 ccgactgggc aatggagcgc ctgccggccc agtatcagcc cgtcatactt gaagctagac
    11101 aggcttatct tggacaagaa gaagatcgct tggcctcgcg cgcagatcag ttggaagaat
    11161 ttgtccacta cgtgaaaggc gagatcacca aggtagtcgg caaataatgt ctagctagaa
    11221 attcgttcaa gccgacgccg cttcgcggcg cggcttaact caagcgttag atgcactaag
    11281 cacataattg ctcacagcca aactatcagg tcaagtctgc ttttattatt tttaagcgtg
    11341 cataataagc cctacacaaa ttgggagata tatcatgcat gaccaaaatc ccttaacgtg
    11401 agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc
    11461 ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg
    11521 tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag
    11581 cgcagatacc aaatactgtc cttctagtgt agccgtagtt aggccaccac ttcaagaact
    11641 ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg
    11701 gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc
    11761 ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg
    11821 aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg
    11881 cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag
    11941 ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc
    12001 gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct
    12061 ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc
    12121 ctgattctgt ggataaccgt attaccgcct ttgagtgagc tgataccgct cgccgcagcc
    12181 gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga agagcgcctg atgcggtatt
    12241 ttctccttac gcatctgtgc ggtatttcac accgcatatg gtgcactctc agtacaatct
    12301 gctctgatgc cgcatagtta agccagtata cactccgcta tcgctacgtg actgggtcat
    12361 ggctgcgccc cgacacccgc caacacccgc tgacgcgccc tgacgggctt gtctgctccc
    12421 ggcatccgct tacagacaag ctgtgaccgt ctccgggagc tgcatgtgtc agaggttttc
    12481 accgtcatca ccgaaacgcg cgaggcaggg tgccttgatg tgggcgccgg cggtcgagtg
    12541 gcgacggcgc ggcttgtccg cgccctggta gattgcctgg ccgtaggcca gccatttttg
    12601 agcggccagc ggccgcgata ggccgacgcg aagcggcggg gcgtagggag cgcagcgacc
    12661 gaagggtagg cgctttttgc agctcttcgg ctgtgcgctg gccagacagt tatgcacagg
    12721 ccaggcgggt tttaagagtt ttaataagtt ttaaagagtt ttaggcggaa aaatcgcctt
    12781 ttttctcttt tatatcagtc acttacatgt gtgaccggtt cccaatgtac ggctttgggt
    12841 tcccaatgta cgggttccgg ttcccaatgt acggctttgg gttcccaatg tacgtgctat
    12901 ccacaggaaa gagacctttt cgaccttttt cccctgctag ggcaatttgc cctagcatct
    12961 gctccgtaca ttaggaaccg gcggatgctt cgccctcgat caggttgcgg tagcgcatga
    13021 ctaggatcgg gccagcctgc cccgcctcct ccttcaaatc gtactccggc aggtcatttg
    13081 acccgatcag cttgcgcacg gtgaaacaga acttcttgaa ctctccggcg ctgccactgc
    13141 gttcgtagat cgtcttgaac aaccatctgg cttctgcctt gcctgcggcg cggcgtgcca
    13201 ggcggtagag aaaacggccg atgccgggat cgatcaaaaa gtaatcgggg tgaaccgtca
    13261 gcacgtccgg gttcttgcct tctgtgatct cgcggtacat ccaatcagct agctcgatct
    13321 cgatgtactc cggccgcccg gtttcgctct ttacgatctt gtagcggcta atcaaggctt
    13381 caccctcgga taccgtcacc aggcggccgt tcttggcctt cttcgtacgc tgcatggcaa
    13441 cgtgcgtggt gtttaaccga atgcaggttt ctaccaggtc gtctttctgc tttccgccat
    13501 cggctcgccg gcagaacttg agtacgtccg caacgtgtgg acggaacacg cggccgggct
    13561 tgtctccctt cccttcccgg tatcggttca tggattcggt tagatgggaa accgccatca
    13621 gtaccaggtc gtaatcccac acactggcca tgccggccgg ccctgcggaa acctctacgt
    13681 gcccgtctgg aagctcgtag cggatcacct cgccagctcg tcggtcacgc ttcgacagac
    13741 ggaaaacggc cacgtccatg atgctgcgac tatcgcgggt gcccacgtca tagagcatcg
    13801 gaacgaaaaa atctggttgc tcgtcgccct tgggcggctt cctaatcgac ggcgcaccgg
    13861 ctgccggcgg ttgccgggat tctttgcgga ttcgatcagc ggccgcttgc cacgattcac
    13921 cggggcgtgc ttctgcctcg atgcgttgcc gctgggcggc ctgcgcggcc ttcaacttct
    13981 ccaccaggtc atcacccagc gccgcgccga tttgtaccgg gccggatggt ttgcgaccgc
    14041 tcacgccgat tcctcgggct tgggggttcc agtgccattg cagggccggc agacaaccca
    14101 gccgcttacg cctggccaac cgcccgttcc tccacacatg gggcattcca cggcgtcggt
    14161 gcctggttgt tcttgatttt ccatgccgcc tcctttagcc gctaaaattc atctactcat
    14221 ttattcattt gctcatttac tctggtagct gcgcgatgta ttcagatagc agctcggtaa
    14281 tggtcttgcc ttggcgtacc gcgtacatct tcagcttggt gtgatcctcc gccggcaact
    14341 gaaagttgac ccgcttcatg gctggcgtgt ctgccaggct ggccaacgtt gcagccttgc
    14401 tgctgcgtgc gctcggacgg ccggcactta gcgtgtttgt gcttttgctc attttctctt
    14461 tacctcatta actcaaatga gttttgattt aatttcagcg gccagcgcct ggacctcgcg
    14521 ggcagcgtcg ccctcgggtt ctgattcaag aacggttgtg ccggcggcgg cagtgcctgg
    14581 gtagctcacg cgctgcgtga tacgggactc aagaatgggc agctcgtacc cggccagcgc
    14641 ctcggcaacc tcaccgccga tgcgcgtgcc tttgatcgcc cgcgacacga caaaggccgc
    14701 ttgtagcctt ccatccgtga cctcaatgcg ctgcttaacc
//
