LOCUS       sequence-207998-       14933 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Arabidopsis transformation, expresses HDT1/GFP fusion with HDT1 
            promoter to study expression pattern in roots.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 14933)
  AUTHORS   Li H, Torres-Garcia J, Latrasse D, Benhamed M, Schilderink S, Zhou 
            W, Kulikova O, Hirt H, Bisseling T
  TITLE     Plant-Specific Histone Deacetylases HDT1/2 Regulate GIBBERELLIN 
            2-OXIDASE2 Expression to Control Arabidopsis Root Meristem Cell 
            Number.
  JOURNAL   Plant Cell. 2017 Sep;29(9):2183-2196. doi: 10.1105/tpc.17.00366. 
            Epub 2017 Aug 30.
  PUBMED    28855334
REFERENCE   2  (bases 1 to 14933)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1105/tpc.17.00366"; journalName: "Plant Cell"; date: "2017-09"; 
            volume: "29"; issue: "9"; pages: "2183-2196"
COMMENT     Sequence Label: pHDT1:HDT1-GFP
FEATURES             Location/Qualifiers
     source          1..14933
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    83..107
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     primer_bind     174..190
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     174..190
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     protein_bind    210..230
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     protein_bind    291..315
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(3382..3406)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             join(3414..3416,3417..3419,3420..4130)
                     /codon_start=1
                     /product="strongly enhanced green fluorescent protein 
                     (Kremers et al., 2007)"
                     /label=SGFP2
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITADKQKNGIK
                     ANFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(3459..3480)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(3720..3739)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     4067..4088
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     protein_bind    complement(4372..4392)
                     /gene="mutant version of attB"
                     /label=attB3
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     primer_bind     complement(4592..4613)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     4804..4823
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     terminator      complement(5105..5357)
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     promoter        7317..7496
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     CDS             7538..8089
                     /codon_start=1
                     /gene="Streptomyces hygroscopicus bar"
                     /product="phosphinothricin acetyltransferase"
                     /label=BlpR
                     /note="confers resistance to bialophos or phosphinothricin"
                     /translation="MSPERRPADIRRATEADMPAVCTIVNHYIETSTVNFRTEPQEPQE
                     WTDDLVRLRERYPWLVAEVDGEVAGIAYAGPWKARNAYDWTAESTVYVSPRHQRTGLGS
                     TLYTHLLKSLEAQGFKSVVAVIGLPNDPSVRMHEALGYAPRGMLRAAGFKHGNWHDVGF
                     WQLDFSLPVPPRPVLPVTEI"
     terminator      8109..8361
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     misc_feature    8454..8478
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             8999..9790
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MGEAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      10036..10624
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     10525..10544
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     10778..10795
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    10810..10950
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(10876..10895)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     11036..11058
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     rep_origin      11294..11488
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     CDS             complement(11554..12627)
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     CDS             complement(13056..13685)
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
ORIGIN
        1 cacatacaaa tggacgaacg gataaacctt ttcacgccct tttaaatatc cgattattct
       61 aataaacgct cttttctctt aggtttaccc gccaatatat cctgtcaaac actgatagtt
      121 taaactgaag gcgggaaacg acaatctgat ccaagctcaa gctaagcttc acacaggaaa
      181 cagctatgac catgattacg ccaagctatc aactttgtat agaaaagttg gccagagacc
      241 aagcttctcg agggtctctc gcgccaaagg gcgcgcctaa cccgggtacc acaagtttgt
      301 acaaaaaagc aggctccgcg gccgccccct tcacccagac ctatctgcta taccaacaca
      361 ttcaatccac ccatatgaac tctcaaattc agcatccaaa caatctgcag catagcgggc
      421 catttcattt gcaaaatgct ggcggaaacg caactgttcc ttgtctgtgc caagacggac
      481 aaggaaaaga tacactctcg caatgaagta gcctacagtt tctttgttca cagttcccta
      541 ttcaaataca gttgcaggca tcaataagca attaagaaac aaaaggtctg agggagtgca
      601 tactagaagt aagtagacgc agatgattac agatactgac cttggcaaca acttcgccaa
      661 ggcaaagttt tttcgcagat tggccagaca tttgttcctc tcttggaaac ataaggaatt
      721 caaaatttgc tacatcagag aatttcggat gtgacttatt cccaggatca acaaaatgct
      781 caatttctgc cagcgtgaat tcacaaactc taagaagccc ttgacgatga gatatctgca
      841 agtaatatgc gtcacttatt aaaagccaga cataaaagaa agaaaaaaga gtccctgatt
      901 gagataaaca catccaatct atcaagcaaa ggaatagaaa attaaacgat aatcacctca
      961 tttctaaagg ttgaccaatt tgagcagcag caaagggaaa tttcctccca ttgtagtaat
     1021 acaagtcctt cacaaaaatg ccttgttaga gtgttcttcg tgtctggagc aataatcctt
     1081 aagcaagtgg tccgtccgca ctgtaacaag ttccagtttt ctcattctta accattagat
     1141 cagtgaactg gtctacatgt ccagatgcct tgagaacaac ctctggtgtc acacatggac
     1201 aatccacttc atacatattc tccttaagaa tgaaatgcta tctgagaaca aacctaaaac
     1261 aatctctact cttctctatg aatcatgtca aatttgtctc aatttctact caaaagagaa
     1321 aggtgaatga tgttccgtac ttacttgacg ccagaagcta agaacattgg atttaacagc
     1381 acaatcctta atcaaatagt ccggcgacgt cacggttgat tttgaaagaa gggatataga
     1441 acaaacgccg ctcgagagta ttaaccacat tttgtcgaaa tcagcagctc catggctgca
     1501 tcaatcgtga agccttgaga gcttgaagac attcccctga gcttcgacgg aggaagattt
     1561 cgcctatact gatttccgcc gtttgaattt gatcggagca ttttgttttt tcacaaactt
     1621 tcatatcttt aacgacgtcg ttagtactga agaaaaaccc taaagagtaa agaccctaac
     1681 ggcgtcgtat taaaacccta aaacggcttc gtattaaaac cctctgtctt ataaataatc
     1741 tcttaaaacc ctttcttctt ctcttacatt ttcagctgct cataaaccct aaaaatcctc
     1801 tctttttctc aaccttgatt cttagccatg gagttctggg gtaagaatct tctctgtttt
     1861 gtctgaagct ttttgggttt ttcttaaaat ctgttttcga actctcttgt ggctgcttcc
     1921 ttgtgtggtg tagttgtgta gcttcatcta gggttttaag ttgcaatatt ctcacataga
     1981 aatggttata ttatatagga attagtgtgg tagtgtataa cttgaagctg tatcttgata
     2041 ctcatgttgg tttttgttaa aacgatgtaa tcaggaattg aagttaaatc aggaaagcca
     2101 gttacagtga ctcctgaaga aggcattctt atccacgttt ctcaggttta gtatcaaatc
     2161 ttattgattt tgattctgag agttgttaga ggtctagtgt aaacaatctt ggattgttta
     2221 tctgtgtagg catcgcttgg agaatgtaaa aacaagaagg gagagtttgt gcctttacat
     2281 gtaaaggttg ggaaccagaa cttggttctg ggaactctat cgactgagaa catccctcag
     2341 cttttctgtg atttggtatt cgacaaggag tttgagcttt ctcacacttg gggaaaagga
     2401 agtgtttact ttgttggata caaaactccc aacattgagc cacaaggcta ttctggtgag
     2461 tttgcaagat aaaatctgtt tcaattcaat tattctgttt gtaactgtaa tattggcttg
     2521 gttgctgatg attcgtttat gtgttgtatt gatatattca gaggaagaag aggaagaaga
     2581 ggaagaagtt cctgctggta cttaaactag acagagacta cttgatttgt gatcctgata
     2641 attggtaata actagttcca tttgcaggga atgctgccaa ggctgtagct aaaccaaagg
     2701 ctaagcctgc agaagtgaag ccagctgttg atgatgaaga ggatgagtct gattctgacg
     2761 gagtgagttg ttatagcttt ctgtgatttg tctctttttg tttaccattt cagaataatg
     2821 actgaggaga tgagacatct tttttgaaca aatgttggtg acgcattctt gtgttatgat
     2881 tcttgcagat ggatgaagat gattctgatg gtgaggattc tgaggaagaa gagcctacac
     2941 ctaagaagcc tgcatcaagc aagaagagag ctaatgaaac tacccctaaa gcacctgtgt
     3001 cagcaaagaa ggcgaaagta gcagttactc ctcagaaaac aggtatagat acaaaccatt
     3061 ctcttacatt attcactaaa ttgggaaaca cgagtattca tttgaacctg catcgtcttc
     3121 tttggcacag atgagaagaa gaaaggggga aaggctgcaa accagagccc aaagtcggcc
     3181 agtcaagtct catgtggttc atgcaagaag tgagactctc tctctttctc taactcttgt
     3241 cttcactgct caaaatttct gtttgttata acatttcgct tgttggctta tatctctttg
     3301 gcaggacttt caactcaggg aatgcacttg agtctcacaa caaggccaag cacgctgctg
     3361 ccaagaaggg tgggcgcgcc gacccagctt tcttgtacaa agtggggcgc gccatggtga
     3421 gcaagggcga ggagctgttc accggggtgg tgcccatcct ggtcgagctg gacggcgacg
     3481 taaacggcca caagttcagc gtgtccggcg agggcgaggg cgatgccacc tacggcaagc
     3541 tgaccctgaa gttcatctgc accaccggca agctgcccgt gccctggccc accctcgtga
     3601 ccaccctgac ctacggcgtg cagtgcttcg cccgctaccc cgaccacatg aagcagcacg
     3661 acttcttcaa gtccgccatg cccgaaggct acgtccagga gcgcaccatc ttcttcaagg
     3721 acgacggcaa ctacaagacc cgcgccgagg tgaagttcga gggcgacacc ctggtgaacc
     3781 gcatcgagct gaagggcatc gacttcaagg aggacggcaa catcctgggg cacaagctgg
     3841 agtacaacta caacagccac aacgtctata tcaccgccga caagcagaag aacggcatca
     3901 aggccaactt caagatccgc cacaacatcg aggacggcgg cgtgcagctc gccgaccact
     3961 accagcagaa cacccccatc ggcgacggcc ccgtgctgct gcccgacaac cactacctga
     4021 gcacccagtc caagctgagc aaagacccca acgagaagcg cgatcacatg gtcctgctgg
     4081 agttcgtgac cgccgccggg atcactctcg gcatggacga gctgtacaag ggtacctaac
     4141 cgcggccatg ctagagtccg caaaaatcac cagtctctct ctacaaatct atctctctct
     4201 atttttctcc agaataatgt gtgagtagtt cccagataag ggaattaggg ttcttatagg
     4261 gtttcgctca tgtgttgagc atataagaaa cccttagtat gtatttgtat ttgtaaaata
     4321 cttctatcaa taaaatttct aattcctaaa accaaaatcc agtgatccgg acaactttat
     4381 tatacatagt tgataattca ctggccgtcg tgacgtcggg cccatcgatg gcgcgctgat
     4441 tgcccttcac cgcctggccc tgagagagtt gcagcaagcg gtccacgctg gtttgcccca
     4501 gcaggcgaaa atcctgtttg atggtggttc cgaaatcggc aaaatccctt ataaatcaaa
     4561 agaatagccc gagatagggt tgagtgttgt tccagtttgg aacaagagtc cactattaaa
     4621 gaacgtggac tccaacgtca aagggcgaaa aaccgtctat cagggcgatg gcccactacg
     4681 tgaaccatca cccaaatcaa gttttttggg gtcgaggtgc cgtaaagcac taaatcggaa
     4741 ccctaaaggg agcccccgat ttagagcttg acggggaaag ccggcgaacg tggcgagaaa
     4801 ggaagggaag aaagcgaaag gagcgggcgc cattcaggcc gatagcagca ccgtaatcag
     4861 tagcgacaga atcaagtttg cctttagcgt cagactgtag cgcgttttca tcggcatttt
     4921 cggtcatagc ccccttatta gcgtttgcca tcttttcata atcaaaatca ccggaaccag
     4981 agccaccacc ggaaccgcct ccctcagagc cgccaccctc agaaccgcca ccctcagagc
     5041 caccaccctc agagccgcca ccagaaccac caccagagcc gccgccagca ttgacaggag
     5101 gcccgatcta gtaacataga tgacaccgcg cgcgataatt tatcctagtt tgcgcgctat
     5161 attttgtttt ctatcgcgta ttaaatgtat aattgcggga ctctaatcat aaaaacccat
     5221 ctcataaata acgtcatgca ttacatgtta attattacat gcttaacgta attcaacaga
     5281 aattatatga taatcatcgc aagaccggca acaggattca atcttaagaa actttattgc
     5341 caaatgtttg aacgatcggg gatccgcttt acttgtacat aaggaacaga tggtggcgtc
     5401 cctcggttct ttcatactgc tcaacgattg tatagtcttc gttgtggctt gttatatcca
     5461 gtttggagtc aacatagtag taccctggta gctgcacagg cttctttgcc atgtaaatac
     5521 ttttgaattc aactaggtaa tgaccaccgt ctttcagctt cagagcctta tgaatctctc
     5581 ctttcaacac gccatcacga ggatacaaac gctcagtgct ggcttcccag cccattgtct
     5641 tcttttgcat aacaggtcca tcggaaggaa agttcacgcc aatgaacttg accttgtaga
     5701 tgaaacagcc atcctgcaaa ctggaatcct gggttacagt aacgacgcca ccgtcttcaa
     5761 agttcatgac cctttcccat ttaaatcctt caggaaatga cagcttttta tagtctggta
     5821 tgtcggcagg gtgcttgaca tataccttgc ttccatactg aaattgtggt gacaaaatat
     5881 cccaagcaaa tggcaaaggt ccccccttgg ttaccttaag ctttacggta ttgtggcctt
     5941 cgtatggcct cccctctcct tcgccttcta tttcaaactc gtgcccattg accgttcctt
     6001 ccatgcgaac cttaaacctc atgaactcct tgataacatt cttggaagac cccatggggt
     6061 atgtttttaa tcttgtttgt attgatgagt tttggtttga gtaaagagtg aagcggatga
     6121 gttaatttat agactataaa ggagatttgc atggcgatca cgtgtaataa tgcatgcacg
     6181 catgtgattg tatgtgtgtg ctgtgagaga gaagctctta ggtgtttgaa gggagtgaca
     6241 agtggcgaag aaaaacaatt ctccgcggct gcatgctatg tgtaacgtgt agctaatgtt
     6301 ctggcatggc atcttatgaa cgattctttt taaaaacaag gtaaaaactt aacttcataa
     6361 aattaaaaaa aagagcgttt actaagttgg tttaaaaggg gatgagagtc tataaatttt
     6421 ggaggtagtg ccgttgggaa tataaattgg gagcttaatc agaattatag aagttaaagt
     6481 tgatttagtc acggtcaata taaattggga atttgagtca aaatcttcca aattcggaat
     6541 ccgtcttgtt acacccggtg gataggagcc gaacggtttg aaaatacttg aaatgtggat
     6601 gcaggtgcag gctggtttaa ttttatgttg aatggataca tgtcaatcga atttgagtta
     6661 taggtacaca ttttactctg atactaaaat gtaacatttg tctcaagaat gggtaggtca
     6721 tccttactcg acaacggtat gccacttcac ttgaacgtat atctcattca acccaactct
     6781 aacacacatt tcatacccat gtctaaaata aagaaaattg aagaaaaacg ctcaatagcg
     6841 tcaatcctta caaatataat gtacatggca acctcgcatg aaaatagtaa taaagaggag
     6901 tgaataatat aaagtagtca ctaattggat gacaaagcaa atggaaccat ccgatagatg
     6961 ttttgagaac aagaggtaca tttgtatata tgcattggtt gatacatttc tgccatctga
     7021 attgtttctt cctcaaagaa gaagcattca gccaatagtg aaatttaaaa tgttggaaaa
     7081 atgaggatgc agtcagaaag tccagaatat aagtggtaac tggtaactga atacttgcct
     7141 cagaagcaag ataagaaaca taagtcttta aaggaatcaa tcaccgatga agagagctcc
     7201 gaatcgaatt cgagctcggt accgcgaatt atcgatcatg agcggagaat taagggagtc
     7261 acgttatgac ccccgccgat gacgcgggac aagccgtttt acgtttggaa ctgacagaac
     7321 cgcaacgttg aaggagccac tgagccgcgg gtttctggag tttaatgagc taagcacata
     7381 cgtcagaaac cattattgcg cgttcaaaag tcgcctaagg tcactatcag ctagcaaata
     7441 tttcttgtca aaaatgctcc actgacgttc cataaattcc cctcggtatc caattagagt
     7501 ctcatattca ctctcaactc gatcgagggg atctaccatg agcccagaac gacgcccggc
     7561 cgacatccgc cgtgccaccg aggcggacat gccggcggtc tgcaccatcg tcaaccacta
     7621 catcgagaca agcacggtca acttccgtac cgagccgcag gaaccgcagg agtggacgga
     7681 cgacctcgtc cgtctgcggg agcgctatcc ctggctcgtc gccgaggtgg acggcgaggt
     7741 cgccggcatc gcctacgcgg gtccctggaa ggcacgcaac gcctacgact ggacggccga
     7801 gtcgaccgtg tacgtctccc cccgccacca gcggacggga ctgggctcca cgctctacac
     7861 ccacctgctg aagtccctgg aggcacaggg cttcaagagc gtggtcgctg tcatcgggct
     7921 gcccaacgac ccgagcgtgc gcatgcacga ggcgctcgga tatgcccccc gcggcatgct
     7981 gcgggcggcc ggcttcaagc acgggaactg gcatgacgtg ggtttctggc agctggactt
     8041 cagcctgccg gtgccgcccc gtccggtcct gcccgtcacc gaaatctgat gacccctaga
     8101 gtcaagcaga tcgttcaaac atttggcaat aaagtttctt aagattgaat cctgttgccg
     8161 gtcttgcgat gattatcata taatttctgt tgaattacgt taagcatgta ataattaaca
     8221 tgtaatgcat gacgttattt atgagatggg tttttatgat tagagtcccg caattataca
     8281 tttaatacgc gatagaaaac aaaatatagc gcgcaaacta ggataaatta tcgcgcgcgg
     8341 tgtcatctat gttactagat cgaccggcat gcaagctgat aattcaattc ggcgttaatt
     8401 cagtacatta aaaacgtccg caatgtgtta ttaagttgtc taagcgtcaa tttgtttaca
     8461 ccacaatata tcctgccacc agccagccaa cagctccccg accggcagct cggcacaaaa
     8521 tcaccactcg atacaggcag cccatcagtc cgggacggcg tcagcgggag agccgttgta
     8581 aggcggcaga ctttgctcat gttaccgatg ctattcggaa gaacggcaac taagctgccg
     8641 ggtttgaaac acggatgatc tcgcggaggg tagcatgttg attgtaacga tgacagagcg
     8701 ttgctgcctg tgatcaattc gggcacgaac ccagtggaca taagcctgtt cggttcgtaa
     8761 gctgtaatgc aagtagcgta tgcgctcacg caactggtcc agaaccttga ccgaacgcag
     8821 cggtggtaac ggcgcagtgg cggttttcat ggcttgttat gactgttttt ttggggtaca
     8881 gtctatgcct cgggcatcca agcagcaagc gcgttacgcc gtgggtcgat gtttgatgtt
     8941 atggagcagc aacgatgtta cgcagcaggg cagtcgccct aaaacaaagt taaacatcat
     9001 gggggaagcg gtgatcgccg aagtatcgac tcaactatca gaggtagttg gcgtcatcga
     9061 gcgccatctc gaaccgacgt tgctggccgt acatttgtac ggctccgcag tggatggcgg
     9121 cctgaagcca cacagtgata ttgatttgct ggttacggtg accgtaaggc ttgatgaaac
     9181 aacgcggcga gctttgatca acgacctttt ggaaacttcg gcttcccctg gagagagcga
     9241 gattctccgc gctgtagaag tcaccattgt tgtgcacgac gacatcattc cgtggcgtta
     9301 tccagctaag cgcgaactgc aatttggaga atggcagcgc aatgacattc ttgcaggtat
     9361 cttcgagcca gccacgatcg acattgatct ggctatcttg ctgacaaaag caagagaaca
     9421 tagcgttgcc ttggtaggtc cagcggcgga ggaactcttt gatccggttc ctgaacagga
     9481 tctatttgag gcgctaaatg aaaccttaac gctatggaac tcgccgcccg actgggctgg
     9541 cgatgagcga aatgtagtgc ttacgttgtc ccgcatttgg tacagcgcag taaccggcaa
     9601 aatcgcgccg aaggatgtcg ctgccgactg ggcaatggag cgcctgccgg cccagtatca
     9661 gcccgtcata cttgaagcta gacaggctta tcttggacaa gaagaagatc gcttggcctc
     9721 gcgcgcagat cagttggaag aatttgtcca ctacgtgaaa ggcgagatca ccaaggtagt
     9781 cggcaaataa tgtctagcta gaaattcgtt caagccgacg ccgcttcgcg gcgcggctta
     9841 actcaagcgt tagatgcact aagcacataa ttgctcacag ccaaactatc aggtcaagtc
     9901 tgcttttatt atttttaagc gtgcataata agccctacac aaattgggag atatatcatg
     9961 catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa
    10021 gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa
    10081 aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc
    10141 gaaggtaact ggcttcagca gagcgcagat accaaatact gtccttctag tgtagccgta
    10201 gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct
    10261 gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg
    10321 atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag
    10381 cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc
    10441 cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg
    10501 agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt
    10561 tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg
    10621 gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca
    10681 catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg
    10741 agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc
    10801 ggaagagcgc ctgatgcggt attttctcct tacgcatctg tgcggtattt cacaccgcat
    10861 atggtgcact ctcagtacaa tctgctctga tgccgcatag ttaagccagt atacactccg
    10921 ctatcgctac gtgactgggt catggctgcg ccccgacacc cgccaacacc cgctgacgcg
    10981 ccctgacggg cttgtctgct cccggcatcc gcttacagac aagctgtgac cgtctccggg
    11041 agctgcatgt gtcagaggtt ttcaccgtca tcaccgaaac gcgcgaggca gggtgccttg
    11101 atgtgggcgc cggcggtcga gtggcgacgg cgcggcttgt ccgcgccctg gtagattgcc
    11161 tggccgtagg ccagccattt ttgagcggcc agcggccgcg ataggccgac gcgaagcggc
    11221 ggggcgtagg gagcgcagcg accgaagggt aggcgctttt tgcagctctt cggctgtgcg
    11281 ctggccagac agttatgcac aggccaggcg ggttttaaga gttttaataa gttttaaaga
    11341 gttttaggcg gaaaaatcgc cttttttctc ttttatatca gtcacttaca tgtgtgaccg
    11401 gttcccaatg tacggctttg ggttcccaat gtacgggttc cggttcccaa tgtacggctt
    11461 tgggttccca atgtacgtgc tatccacagg aaagagacct tttcgacctt tttcccctgc
    11521 tagggcaatt tgccctagca tctgctccgt acattaggaa ccggcggatg cttcgccctc
    11581 gatcaggttg cggtagcgca tgactaggat cgggccagcc tgccccgcct cctccttcaa
    11641 atcgtactcc ggcaggtcat ttgacccgat cagcttgcgc acggtgaaac agaacttctt
    11701 gaactctccg gcgctgccac tgcgttcgta gatcgtcttg aacaaccatc tggcttctgc
    11761 cttgcctgcg gcgcggcgtg ccaggcggta gagaaaacgg ccgatgccgg gatcgatcaa
    11821 aaagtaatcg gggtgaaccg tcagcacgtc cgggttcttg ccttctgtga tctcgcggta
    11881 catccaatca gctagctcga tctcgatgta ctccggccgc ccggtttcgc tctttacgat
    11941 cttgtagcgg ctaatcaagg cttcaccctc ggataccgtc accaggcggc cgttcttggc
    12001 cttcttcgta cgctgcatgg caacgtgcgt ggtgtttaac cgaatgcagg tttctaccag
    12061 gtcgtctttc tgctttccgc catcggctcg ccggcagaac ttgagtacgt ccgcaacgtg
    12121 tggacggaac acgcggccgg gcttgtctcc cttcccttcc cggtatcggt tcatggattc
    12181 ggttagatgg gaaaccgcca tcagtaccag gtcgtaatcc cacacactgg ccatgccggc
    12241 cggccctgcg gaaacctcta cgtgcccgtc tggaagctcg tagcggatca cctcgccagc
    12301 tcgtcggtca cgcttcgaca gacggaaaac ggccacgtcc atgatgctgc gactatcgcg
    12361 ggtgcccacg tcatagagca tcggaacgaa aaaatctggt tgctcgtcgc ccttgggcgg
    12421 cttcctaatc gacggcgcac cggctgccgg cggttgccgg gattctttgc ggattcgatc
    12481 agcggccgct tgccacgatt caccggggcg tgcttctgcc tcgatgcgtt gccgctgggc
    12541 ggcctgcgcg gccttcaact tctccaccag gtcatcaccc agcgccgcgc cgatttgtac
    12601 cgggccggat ggtttgcgac cgctcacgcc gattcctcgg gcttgggggt tccagtgcca
    12661 ttgcagggcc ggcagacaac ccagccgctt acgcctggcc aaccgcccgt tcctccacac
    12721 atggggcatt ccacggcgtc ggtgcctggt tgttcttgat tttccatgcc gcctccttta
    12781 gccgctaaaa ttcatctact catttattca tttgctcatt tactctggta gctgcgcgat
    12841 gtattcagat agcagctcgg taatggtctt gccttggcgt accgcgtaca tcttcagctt
    12901 ggtgtgatcc tccgccggca actgaaagtt gacccgcttc atggctggcg tgtctgccag
    12961 gctggccaac gttgcagcct tgctgctgcg tgcgctcgga cggccggcac ttagcgtgtt
    13021 tgtgcttttg ctcattttct ctttacctca ttaactcaaa tgagttttga tttaatttca
    13081 gcggccagcg cctggacctc gcgggcagcg tcgccctcgg gttctgattc aagaacggtt
    13141 gtgccggcgg cggcagtgcc tgggtagctc acgcgctgcg tgatacggga ctcaagaatg
    13201 ggcagctcgt acccggccag cgcctcggca acctcaccgc cgatgcgcgt gcctttgatc
    13261 gcccgcgaca cgacaaaggc cgcttgtagc cttccatccg tgacctcaat gcgctgctta
    13321 accagctcca ccaggtcggc ggtggcccat atgtcgtaag ggcttggctg caccggaatc
    13381 agcacgaagt cggctgcctt gatcgcggac acagccaagt ccgccgcctg gggcgctccg
    13441 tcgatcacta cgaagtcgcg ccggccgatg gccttcacgt cgcggtcaat cgtcgggcgg
    13501 tcgatgccga caacggttag cggttgatct tcccgcacgg ccgcccaatc gcgggcactg
    13561 ccctggggat cggaatcgac taacagaaca tcggccccgg cgagttgcag ggcgcgggct
    13621 agatgggttg cgatggtcgt cttgcctgac ccgcctttct ggttaagtac agcgataacc
    13681 ttcatgcgtt ccccttgcgt atttgtttat ttactcatcg catcatatac gcagcgaccg
    13741 catgacgcaa gctgttttac tcaaatacac atcacctttt tagacggcgg cgctcggttt
    13801 cttcagcggc caagctggcc ggccaggccg ccagcttggc atcagacaaa ccggccagga
    13861 tttcatgcag ccgcacggtt gagacgtgcg cgggcggctc gaacacgtac ccggccgcga
    13921 tcatctccgc ctcgatctct tcggtaatga aaaacggttc gtcctggccg tcctggtgcg
    13981 gtttcatgct tgttcctctt ggcgttcatt ctcggcggcc gccagggcgt cggcctcggt
    14041 caatgcgtcc tcacggaagg caccgcgccg cctggcctcg gtgggcgtca cttcctcgct
    14101 gcgctcaagt gcgcggtaca gggtcgagcg atgcacgcca agcagtgcag ccgcctcttt
    14161 cacggtgcgg ccttcctggt cgatcagctc gcgggcgtgc gcgatctgtg ccggggtgag
    14221 ggtagggcgg gggccaaact tcacgcctcg ggccttggcg gcctcgcgcc cgctccgggt
    14281 gcggtcgatg attagggaac gctcgaactc ggcaatgccg gcgaacacgg tcaacaccat
    14341 gcggccggcc ggcgtggtgg tgtcggccca cggctctgcc aggctacgca ggcccgcgcc
    14401 ggcctcctgg atgcgctcgg caatgtccag taggtcgcgg gtgctgcggg ccaggcggtc
    14461 tagcctggtc actgtcacaa cgtcgccagg gcgtaggtgg tcaagcatcc tggccagctc
    14521 cgggcggtcg cgcctggtgc cggtgatctt ctcggaaaac agcttggtgc agccggccgc
    14581 gtgcagttcg gcccgttggt tggtcaagtc ctggtcgtcg gtgctgacgc gggcatagcc
    14641 cagcaggcca gcggcggcgc tcttgttcat ggcgtaatgt ctccggttct agtcgcaagt
    14701 attctacttt atgcgactaa aacacgcgac aagaaaacgc caggaaaagg gcagggcggc
    14761 agcctgtcgc gtaacttagg acttgtgcga catgtcgttt tcagaagacg gctgcactga
    14821 acgtcagaag ccgactgcac tatagcagcg gaggggttgg atcaaagtac tttaaagtac
    14881 tttaaagtac tttaaagtac tttgatcccg aggggaaccc tgtggttggc atg
//
