LOCUS       sequence-208-exp        9875 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9875)
  AUTHORS   Fire A et al
  TITLE     Fire Lab C. Elegans Vector Kit 1997
REFERENCE   2  (bases 1 to 9875)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: L3808
FEATURES             Location/Qualifiers
     source          1..9875
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(2505..2533)
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
     primer_bind     3281..3301
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
     promoter        7620..7724
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7725..7793,7794..8585)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7943..7962)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      8756..9344
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9245..9264
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     9498..9515
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    9632..9653
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(9668..9673,9674..9691,9692..9698)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9706..9722
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     9711..9733
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     9730..9746
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     9730..9746
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 aagcttgggc tgcaggtcgg ctataataag ttcttgaata aaataatttt cccgacaaaa
       61 catgagtatt tctttcgaaa ataaaagtgc aggctaatta gagattattc tgtaattaac
      121 tgcataattt gtcacgtgcc atagttttac attccactac gtcatagttc ttaaaatact
      181 aatctcctga aaatagaagt aggtgaagaa agtttaatta tcagttctaa aatgacaatt
      241 gatctttgga atatgttctg aaactaccga tcattgaaca gatgctattt gaatgatata
      301 gaattgtata tttgcaattt ctgaaacgcg ttcttaaagg cacacagatt aattcaaaag
      361 ggtctggccg caaaaaggtt tatggtggcc gattttgagt tttgtgtgtg attgcttttt
      421 cacaatcagt gttttcagga ttatgtgatg aactagatct tcaagtttcg ttacatttca
      481 tatgttttcg gaactcacga agtacatatt gggtattgtg ctcaaaaaat tcagcaatca
      541 gcttcgctcc gctgacttta gaacccaaaa aaatagtatg gccaaactga ctgtgttacg
      601 atcatttcaa tttttcaata catatttaag atttctaaga gtaagaaggt caaaaactgt
      661 tctggaatac atatatattt ttcaggttac aattagtcaa aaagtgcact gaaatatacg
      721 ttttaatttc acgaataacc caattagttc aatgtatttt tggtcaacca acgttaaagt
      781 ttggcttcca accaattatc atttctgatc aaccacaatg ttttttcttt atctgcaagt
      841 taatttttta tttttatcca gatgtttggc atatttttca attcttcact agcgcccact
      901 tcttgcactt ccggcgccct gaatctaatg catctgttgc aagaattgaa agaccaatca
      961 acacattgtt ttcttcacga gatactgaag aaaatgaata aaaacagaga aaaagagcca
     1021 tgtgattagt gacaactgtt gctaacagat accatagctt ggacttggta cgtgatggca
     1081 acgtatgggt caacaaaaat gattgcagag ggggtgcaaa acagtcaagt cgagaaaata
     1141 tgaaaaacag aaaacaaaga acagaaaaat gggtttgaga gtcagtataa tttataaaag
     1201 aaaaattgta catagaaatt aaccattttt gtagaagaag ttatttttca agcatcgtta
     1261 aaaattattc aaagcacctt atttcatatt taattttaaa catggttaaa tgaacaacac
     1321 ggtgcgcaat caggaaaact tgaaatctga aactgttgtt gtgatcttct tcgcaactgt
     1381 tcagatagca ctagtgtaat gttaagagtg cgcgaatata atggaatata atggatcaca
     1441 cctcctgcca tcaggtaaac gtctctgtta tcacatattt ccaactatta aatttttacc
     1501 ttttacagtt ttacattttt ttgaaaaaag taactttttg tcttcaaaat ccctgacgaa
     1561 aatatcaaat attttaatcg agactgcaga ggaaccgatt gatgatttgg aaaatccagc
     1621 tttacctgtg taagaactga aaagtttcat aaccctaggg tattcccagt tacattcccc
     1681 actggctaac aatagcaccc agtttttcat cacctttctt caaatttctc ggcgatttgt
     1741 taaaaacaaa atttgtgtcc cttctctgat atctctatgt ctctaaacac aagttcatcg
     1801 gaaaacgaag gagggtaggt gttggttggg ctcccgaagt gaaaatagaa gagcaagaat
     1861 agaatattag agagagagtg cagagagggc gggatagctc ccgggattcc gttttcttct
     1921 tctttatctt caacgatgat gtgtgtgcgt gttgtataga ttctgttgct cccccacaac
     1981 tcgctccgaa ggctcaatac aattcaattg atattggagg agagcctacc ggagtgggag
     2041 gataagaaga aacataagaa gaagaagaag aagaagcatg cttctggttt ttgatgctat
     2101 gaaaacggca caaaaagatg attgaggtcc cttttcaata ccttctctca tctttcaaat
     2161 cccattgaaa cctaaaactt ctcaccacgc tttaccattg ttctccaaaa acttatagca
     2221 atgtctataa cttttttatc tctgaaaagc agtgttccat ttttcttttt cctattttat
     2281 ttcaattgtt tctcacattt cgtttggatt ctttgcttgt caaccagctt cttcttccac
     2341 ttttaccgtc taattttcag ggcagggagc catcaaaccc acgaccacta gatccatcta
     2401 gaggatccta tcgattcgcg gccgctgtac acccgggtgc cctatagtga gtcgtattgg
     2461 taccggtaga aaaaatgagt aaaggagaag aacttttcac tggagttgtc ccaattcttg
     2521 ttgaattaga tggtgatgtt aatgggcaca aattttctgt cagtggagag ggtgaaggtg
     2581 atgcaacata cggaaaactt acccttaaat ttatttgcac tactggaaaa ctacctgttc
     2641 catgggtaag tttaaacata tatatactaa ctaaccctga ttatttaaat tttcagccaa
     2701 cacttgtcac tactttctgt tatggtgttc aatgcttctc gagataccca gatcatatga
     2761 aacagcatga ctttttcaag agtgccatgc ccgaaggtta tgtacaggaa agaactatat
     2821 ttttcaaaga tgacgggaac tacaagacac gtaagtttaa acagttcggt actaactaac
     2881 catacatatt taaattttca ggtgctgaag tcaagtttga aggtgatacc cttgttaata
     2941 gaatcgagtt aaaaggtatt gattttaaag aagatggaaa cattcttgga cacaaattgg
     3001 aatacaacta taactcacac aatgtataca tcatggcaga caaacaaaag aatggaatca
     3061 aagttgtaag tttaaacatg attttactaa ctaactaatc tgatttaaat tttcagaact
     3121 tcaaaattag acacaacatt gaagatggaa gcgttcaact agcagaccat tatcaacaaa
     3181 atactccaat tggcgatggc cctgtccttt taccagacaa ccattacctg tccacacaat
     3241 ctgccctttc gaaagatccc aacgaaaaga gagaccacat ggtccttctt gagtttgtaa
     3301 cagctgctgg gattacacat ggcatggacg aactatacaa aagcccacaa gctttggaat
     3361 tcgctagccg gccatacaag taatccgggt tctagaagcg ctgctaaggg ggccctcgtc
     3421 gagtcggtca caatcacctg aaactccaaa ggcagccagt gaggaacgtg aagaagaaga
     3481 aaaagagtca tctgaacagg tttgattttc tttctggtca aaaagatgaa attattgatt
     3541 ttcagccaga tactcccaaa actagcagcg agaagtctgc aagtcgttca cagtcgccca
     3601 gagaatcgcg ggaagtgagc caagaggtat gtttttcaaa aatcaataac tgatcataat
     3661 ttttattgtt tggtgaattt aagaaaataa tattcgaaaa ttcctctgaa ttatcaagat
     3721 tgcagtatta atttcgagaa aaattgagat attcatagag ctattgtaaa ttttcttgat
     3781 ttcagactga aacttcggaa aatcaagaga aaatcaaaga aaaggatgac ggggatgatc
     3841 agcctggcac accgaacagc tatagaagcc gggaaacttc accagctcca aaaaggtcca
     3901 aggagaccag gtttgtcaaa agcttcctgc gattaattct catttcaatt tttcagagaa
     3961 tcagagtctc ctgaaaaatc cccggttcgt tcaagatctc ccagaaggtc ttcagcacgt
     4021 tccccgtcac gatctcctag acggcgccga gaaagaagct cagaaagaaa gcaatccgaa
     4081 gagccagcac cgctaccaga gaaaaagaag aaagagccgc tggatattct acgaacaaga
     4141 accggaggag catatattcc acccgccaaa cttcgactta tgcaacaaca gattagtgat
     4201 aagcaaagtg aacagtatca gagaatgaat tgggaaagaa tgaagaaaaa gattcacgga
     4261 ttggttaaca gagtcaacgc gaagaatctt gttcaaattg tcagagaact tcttcaagag
     4321 aatgtgattc gttcaaagtg agtgagaaaa tcgaaggaaa aggaaagaat taatttaatt
     4381 tttcagggga cttctctgcc gtgacattat tcaagctcag gctttctcac caggattctc
     4441 taacgtctat gcagctttgg cggcagttat caactcgaaa ttccctcatg tcggtgaact
     4501 tcttctccgt cgtctgattg tacagttcaa aagaagtttc cgtagaaatg acagaggcgt
     4561 cacggtgaac gtgatcaaat tcatcgcaca tttgattaat caacaagttg ctcacgaagt
     4621 tcttgcgctg gaaatcatga ttctgatgct tgaagaacca actgatgatt cagttgaagt
     4681 cgccattgcg ttcctgaaag agtgtggagc aaagcttctg gagattgctc cagcagctct
     4741 taacagtgtc tacgaccgtc ttcgtgcaat tctcatggaa actgaaagat cggaaaatgc
     4801 actggatcga cgtattcagt atatgattga gactgcaatg cagattcgaa aggacaaatt
     4861 tgcggcgtat ccggcggtca ttgaagactt ggacttgatt gaggaggagg atcagatcat
     4921 ccatacactt aatttggagg atgcggttga tccggaaaat gggcttaatg tgttcaaact
     4981 agatccagaa ttcgaaaaga acgaggaggt ttatgaggag atccgtaagg aaatcattgg
     5041 aaacgccgat atttcggatg aggatggtgg cgacgagttg gatgatgaag aagagggtag
     5101 tgatgtggaa gaggctccga agaagactac agagattatt gataatactg atcagaattg
     5161 actgctttca gaaggtattc attttgagtt ttgggccggc aaatctgtaa gttgccggtt
     5221 gccgaaaatt tgctgaattt gccggaaaaa aaaattccgg aatttattta aaaacttttt
     5281 gtaaaaatta aattaaattt gcaacttttc agagaagtct acctgacaat gcaatcatct
     5341 ttggactacc aagaagctgc tcacaaattg ctgaaaatga agattccaga cagcatgcag
     5401 gtcagcgatg ttgcaaagaa aaattttcga ccaaaaaaac caaccaatca taaaatttaa
     5461 aaaaaaactc cgtttttttc ttttttttta tacgagaaaa accaaaaaaa tgtatttttg
     5521 ccaaattcta aaatactatc cccgaaattt tcaatatttt ctctttcaga acgaactctg
     5581 cgcgatgctt gtcgattgtt gtgctcaaca gcgtacctac gagcgattct acggaatgct
     5641 catcgaacgt ttctgccgac ttcgcctcga ataccagcaa tactttgaaa agctctgcca
     5701 ggacacgtat tccacgattc accgaattga catcacaaaa ctgcggaatt tggctcgcct
     5761 tattgctcat ttgctctcga cggatgctat tgactggaag attttggccg atatgaaaat
     5821 gaccgaagag gacacaactt cttctggcag aatctatatt aaatatatat ttaatgaact
     5881 tgtggaggcg atgggaatgg ttaaacttca ttcgagagtt actgatccgt gagtttccta
     5941 gagagagttg ttttcgtatt caattttccc tattttcaga actttggctc attgctttgt
     6001 tggattattc ccacgaacta atccgaacag cgcacgattt tcgatcaact tcttcacaat
     6061 gattggattg ggtggtttga cgttggaact tcgtgaatgg ctggcaaagg gtctcaagaa
     6121 gaagaaggga atgctggatc agttgaaggc cgaatcaagc tcagattcat cgtcgtcttc
     6181 ggattcgtca gactcgtctg attcttcgga ttctgacgat tcatccgact cgtcttcaga
     6241 ttcctcatct tcttcagaat cagagccaga accaccgaag aaaaagaaga agaagaacag
     6301 tgaagagagt tccaaaaaga aggaaaaaga gaatattggt cgacgggatc gtggagacaa
     6361 gagagctgaa cgtcatcgtg atcaaagtgt ggagaacaag gacaaggatc gtcgacgtcg
     6421 ccaggattct gacgaaaatc gtcggccaga acgaggagat gaccgcaagg atcggagtaa
     6481 agatcgtcgt cgtcaagact cggatgatga ggatcggaaa ggtcgtgaac gtcgggaaga
     6541 ttcaggggaa agacgtcgcg gagatcggga tcgacgtgat cgaaacaagg atcaggagga
     6601 tcaccgtgaa gatcgccgtg accgaagcaa ggatcgtgag gatcgacgtg atcgccgtcg
     6661 tcatgactct gatgatgatc gtaaaactcg tcgggataga agtgaagagc gaggaggacg
     6721 tcgtcgtgaa gtggaatcgg atgatcgacg ccgacgtcgt tgaattttca aattttaaat
     6781 actgaatatt tgtttttttt cctattattt atttattctc tttgtgtttt ttttcttgct
     6841 ttctaaaaaa ttaattcaat ccaaatctaa acattttttt ttctctttcc gtctcccaat
     6901 tcgtattccg ctcctctcat ctgaacacaa tgtgcaagtt tatttatctt ctcgctttca
     6961 tttcattagg acgtgggggg aattggtgga agggggaaac acacaaaagg atgatggaaa
     7021 tgaaataagg acacacaata tgcaacaaca ttcaattcag aaatatggag gaaggtttaa
     7081 aagaaaacat aaaaatatat agaggaggaa ggaaaactag taaaaaataa gcaaagaaat
     7141 taggcgaacg atgagaattg tcctcgcttg gcaaatgcga atccgtatgg agaggcacgt
     7201 ttggcgaagg caaatgttcg gtatggagat ctgtaaaaat ttttaagttg aaatttggtg
     7261 ttgctctttt acaaaatttt ccgattttcg cttgaaatta cggtgccagg tctcgacacg
     7321 tcttccaatt tttcaaattc aaaagagcct ttaatgggct gtagttgcta atttctcgtt
     7381 tttgaaaatt tttcttccgt ttaatcgaaa tttgatgtat tttatttatg attttcaata
     7441 aatttcaaag aaactggtga aaactcggaa aattgtgaac tacagtaatc caatccttaa
     7501 aggcgcacac cttttaaatg tccgccccaa tacgatattt ttttaagatt cgctagagcg
     7561 gcccaaaagg cctttttttt ttggcgccga cgtcaggtgg cacttttcgg ggaaatgtgc
     7621 gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac
     7681 aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt attcaacatt
     7741 tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt gctcacccag
     7801 aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg ggttacatcg
     7861 aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa cgttttccaa
     7921 tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtatt gacgccgggc
     7981 aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag tactcaccag
     8041 tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt gctgccataa
     8101 ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga ccgaaggagc
     8161 taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt tgggaaccgg
     8221 agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgta gcaatggcaa
     8281 caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg caacaattaa
     8341 tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc cttccggctg
     8401 gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt atcattgcag
     8461 cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg gggagtcagg
     8521 caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg attaagcatt
     8581 ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa cttcattttt
     8641 aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac
     8701 gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag
     8761 atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg
     8821 tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca
     8881 gagcgcagat accaaatact gtccttctag tgtagccgta gttaggccac cacttcaaga
     8941 actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca
     9001 gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc
     9061 agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca
     9121 ccgaactgag atacctacag cgtgagcatt gagaaagcgc cacgcttccc gaagggagaa
     9181 aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc
     9241 cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc
     9301 gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg
     9361 cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat
     9421 cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc gctcgccgca
     9481 gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc ccaatacgca
     9541 aaccgcctct ccccgcgcgt tggccgattc attaatgcag ctggcacgac aggtttcccg
     9601 actggaaagc gggcagtgag cgcaacgcaa ttaatgtgag ttagctcact cattaggcac
     9661 cccaggcttt acactttatg cttccggctc gtatgttgtg tggaattgtg agcggataac
     9721 aatttcacac aggaaacagc tatgaccatg attacgccaa gctgtaagtt taaacatgat
     9781 cttactaact aactattctc atttaaattt tcagagctta aaaatggctg aaatcactca
     9841 caacgatgga tacgctaaca acttggaaat gaaat
//
