LOCUS       sequence-208000-        8282 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mammalian expression vector for Streptococcus thermophilus CRISPR1 
            (St1Cas9 LMD-9) .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8282)
  AUTHORS   Agudelo D, Carter S, Velimirovic M, Duringer A, Rivest JF, Levesque 
            S, Loehr J, Mouchiroud M, Cyr D, Waters PJ, Laplante M, Moineau S, 
            Goulet A, Doyon Y
  TITLE     Versatile and robust genome editing with Streptococcus thermophilus 
            CRISPR1-Cas9.
  JOURNAL   Genome Res. 2020 Jan;30(1):107-117. doi: 10.1101/gr.255414.119. Epub
            2020 Jan 3.
  PUBMED    31900288
REFERENCE   2  (bases 1 to 8282)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1101/gr.255414.119"; journalName: "Genome Res"; date: "2020-01";
            volume: "30"; issue: "1"; pages: "107-117"
COMMENT     Sequence Label: MSP1594_2x_NLS
FEATURES             Location/Qualifiers
     source          1..8282
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        4..383
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        386..661
                     /label=chicken beta-actin promoter
     intron          662..1670
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     1594..1616
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     1678..1697
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     regulatory      1763..1772
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1778..1798
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             1823..5182
                     /codon_start=1
                     /product="Cas9 endonuclease from the Streptococcus 
                     thermophilus Type II CRISPR/Cas system (Esvelt et al., 
                     2013)"
                     /label=St1Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="SDLVLGLDIGIGSVGVGILNKVTGEIIHKNSRIFPAAQAENNLVR
                     RTNRQGRRLARRKKHRRVRLNRLFEESGLITDFTKISINLNPYQLRVKGLTDELSNEEL
                     FIALKNMVKHRGISYLDDASDDGNSSVGDYAQIVKENSKQLETKTPGQIQLERYQTYGQ
                     LRGDFTVEKDGKKHRLINVFPTSAYRSEALRILQTQQEFNPQITDEFINRYLEILTGKR
                     KYYHGPGNEKSRTDYGRYRTSGETLDNIFGILIGKCTFYPDEFRAAKASYTAQEFNLLN
                     DLNNLTVPTETKKLSKEQKNQIINYVKNEKAMGPAKLFKYIAKLLSCDVADIKGYRIDK
                     SGKAEIHTFEAYRKMKTLETLDIEQMDRETLDKLAYVLTLNTEREGIQEALEHEFADGS
                     FSQKQVDELVQFRKANSSIFGKGWHNFSVKLMMELIPELYETSEEQMTILTRLGKQKTT
                     SSSNKTKYIDEKLLTEEIYNPVVAKSVRQAIKIVNAAIKEYGDFDNIVIEMARETNEDD
                     EKKAIQKIQKANKDEKDAAMLKAANQYNGKAELPHSVFHGHKQLATKIRLWHQQGERCL
                     YTGKTISIHDLINNSNQFEVDHILPLSITFDDSLANKVLVYATANQEKGQRTPYQALDS
                     MDDAWSFRELKAFVRESKTLSNKKKEYLLTEEDISKFDVRKKFIERNLVDTRYASRVVL
                     NALQEHFRAHKIDTKVSVVRGQFTSQLRRHWGIEKTRDTYHHHAVDALIIAASSQLNLW
                     KKQKNTLVSYSEDQLLDIETGELISDDEYKESVFKAPYQHFVDTLKSKEFEDSILFSYQ
                     VDSKFNRKISDATIYATRQAKVGKDKADETYVLGKIKDIYTQDGYDAFMKIYKKDKSKF
                     LMYRHDPQTFEKVIEPILENYPNKQINEKGKEVPCNPFLKYKEEHGYIRKYSKKGNGPE
                     IKSLKYYDSKLGNHIDITPKDSNNKVVLQSVSPWRADVYFNKTTGKYEILGLKYADLQF
                     EKGTGTYKISQEKYNDIKKKEGVDSDSEFKFTLYKNDLLLVKDTETKEQQLFRFLSRTM
                     PKQKHYVELKPYDKQKFEGGEALIKVLGNVANSGQCKKGLGKSNISIYKVRTDVLGNQH
                     IIKNEGDKPKLDF"
     CDS             5195..5215
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     primer_bind     complement(5304..5323)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    5369..5424
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(5423..5442)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(5785..5801)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5785..5801)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(5798..5820)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    5809..5825
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(5833..5839,5840..5857,5858..5863))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5878..5899
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        5958..6153
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      6004..6139
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     polyA_signal    6159..6293
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6196..6215)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6250..6269
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(6361..6378)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6532..7120)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6612..6631)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7291..8082,8083..8151))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7914..7933
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8152..8256)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gtcgacattg attattgact agttattaat agtaatcaat tacggggtca ttagttcata
       61 gcccatatat ggagttccgc gttacataac ttacggtaaa tggcccgcct ggctgaccgc
      121 ccaacgaccc ccgcccattg acgtcaataa tgacgtatgt tcccatagta acgccaatag
      181 ggactttcca ttgacgtcaa tgggtggact atttacggta aactgcccac ttggcagtac
      241 atcaagtgta tcatatgcca agtacgcccc ctattgacgt caatgacggt aaatggcccg
      301 cctggcatta tgcccagtac atgaccttat gggactttcc tacttggcag tacatctacg
      361 tattagtcat cgctattacc atgggtcgag gtgagcccca cgttctgctt cactctcccc
      421 atctcccccc cctccccacc cccaattttg tatttattta ttttttaatt attttgtgca
      481 gcgatggggg cggggggggg gggggcgcgc gccaggcggg gcggggcggg gcgaggggcg
      541 gggcggggcg aggcggagag gtgcggcggc agccaatcag agcggcgcgc tccgaaagtt
      601 tccttttatg gcgaggcggc ggcggcggcg gccctataaa aagcgaagcg cgcggcgggc
      661 gggagtcgct gcgttgcctt cgccccgtgc cccgctccgc gccgcctcgc gccgcccgcc
      721 ccggctctga ctgaccgcgt tactcccaca ggtgagcggg cgggacggcc cttctcctcc
      781 gggctgtaat tagcgcttgg tttaatgacg gctcgtttct tttctgtggc tgcgtgaaag
      841 ccttaaaggg ctccgggagg gccctttgtg cgggggggag cggctcgggg ggtgcgtgcg
      901 tgtgtgtgtg cgtggggagc gccgcgtgcg gcccgcgctg cccggcggct gtgagcgctg
      961 cgggcgcggc gcggggcttt gtgcgctccg cgtgtgcgcg aggggagcgc ggccgggggc
     1021 ggtgccccgc ggtgcggggg ggctgcgagg ggaacaaagg ctgcgtgcgg ggtgtgtgcg
     1081 tgggggggtg agcagggggt gtgggcgcgg cggtcgggct gtaacccccc cctgcacccc
     1141 cctccccgag ttgctgagca cggcccggct tcgggtgcgg ggctccgtgc ggggcgtggc
     1201 gcggggctcg ccgtgccggg cggggggtgg cggcaggtgg gggtgccggg cggggcgggg
     1261 ccgcctcggg ccggggaggg ctcgggggag gggcgcggcg gccccggagc gccggcggct
     1321 gtcgaggcgc ggcgagccgc agccattgcc ttttatggta atcgtgcgag agggcgcagg
     1381 gacttccttt gtcccaaatc tggcggagcc gaaatctggg aggcgccgcc gcaccccctc
     1441 tagcgggcgc gggcgaagcg gtgcggcgcc ggcaggaagg aaatgggcgg ggagggcctt
     1501 cgtgcgtcgc cgcgccgccg tccccttctc catctccagc ctcggggctg ccgcaggggg
     1561 acggctgcct tcggggggga cggggcaggg cggggttcgg cttctggcgt gtgaccggcg
     1621 gctctagagc ctctgctaac catgttcatg ccttcttctt tttcctacag ctcctgggca
     1681 acgtgctggt tattgtgctg tctcatcatt ttggcaaaga attctgcagt cgacggtacc
     1741 gcgggcccgg gatccaccgg tcgccaccat gggcgcccca aagaagaagc ggaaggtcgg
     1801 tatccacgga gtcccagcag ccagcgacct ggtgctgggc ctggacatcg gcatcggcag
     1861 cgtgggcgtg ggcatcctga acaaggtgac cggcgagatc atccacaaga acagtcgcat
     1921 cttccctgct gctcaggctg agaacaacct ggtgcgccgc accaaccgcc agggtcgccg
     1981 gcttgctcgc cgcaagaagc accggcgcgt gcgcctgaac cgcctgttcg aggagagcgg
     2041 cctgatcacc gacttcacca agatcagcat caacctgaac ccctaccagc tgcgcgtgaa
     2101 gggcctgacc gacgagctga gcaacgagga gctgttcatc gccctgaaga acatggtgaa
     2161 gcaccgcggc atcagctacc tggacgacgc cagcgacgac ggcaacagca gcgtgggcga
     2221 ctacgcccag atcgtgaagg agaacagcaa gcagctggag accaagaccc ccggccagat
     2281 ccagctggag cgctaccaga cctacggcca gctgcgcggc gacttcaccg tggagaagga
     2341 cggcaagaag caccgcctga tcaacgtgtt ccccaccagc gcctaccgca gcgaggccct
     2401 gcgcatcctg cagacccagc aggagttcaa cccccagatc accgacgagt tcatcaaccg
     2461 ctacctggag atcctgaccg gcaagcgcaa gtactaccac ggccccggca acgagaagag
     2521 ccgcaccgac tacggccgct accgcaccag cggcgagacc ctggacaaca tcttcggcat
     2581 cctgatcggc aagtgcacct tctaccccga cgagttccgc gccgccaagg ccagctacac
     2641 cgcccaggag ttcaatctgc tgaacgacct gaacaacctg accgtgccca ccgagaccaa
     2701 gaagctgagc aaggagcaga agaaccagat catcaactac gtgaagaacg agaaggctat
     2761 gggccccgcc aagctgttca agtacatcgc caagctgctg agctgcgacg tggccgacat
     2821 caagggctac cgcatcgaca agagcggcaa ggccgagatc cacaccttcg aggcctaccg
     2881 caagatgaag accctggaga ccctggacat cgagcagatg gaccgagaga ccctggacaa
     2941 gctggcctac gtgctgaccc tgaacaccga gcgcgagggc atccaggagg ccctggagca
     3001 cgagttcgcc gacggcagct tcagccagaa acaggtggac gagctggtgc agttccgcaa
     3061 ggccaacagc agcatcttcg gcaagggctg gcacaacttc agcgtgaagc tgatgatgga
     3121 gctgatcccc gagctgtacg agaccagcga ggagcagatg accatcctga cccgcctggg
     3181 caagcagaag accaccagca gcagcaacaa gaccaagtac atcgacgaga agctgctgac
     3241 cgaggagatc tacaaccccg tggtggccaa gagcgtgcgc caggccatca agatcgtgaa
     3301 cgccgccatc aaggagtacg gcgacttcga caacatcgtg atcgagatgg cccgcgagac
     3361 caacgaggac gacgagaaga aggccatcca gaagatccag aaggccaaca aggacgagaa
     3421 ggacgccgcc atgctgaagg ccgccaacca gtacaacggc aaggccgagc tgccccacag
     3481 cgtgttccac ggccacaagc agctggccac caagatccgc ctgtggcacc agcagggcga
     3541 gcgctgcctg tacaccggca agaccatcag catccacgac ctgatcaaca acagcaacca
     3601 gttcgaggtg gaccacatcc tgcccctgag catcaccttc gacgacagcc tggccaacaa
     3661 ggtgctggtg tacgccaccg ccaaccagga gaagggccag cgcaccccct accaggccct
     3721 ggacagcatg gacgacgcct ggagcttccg cgagctgaag gccttcgtgc gcgagagcaa
     3781 gaccctgagc aacaagaaga aggagtatct gctgaccgag gaggacatca gcaagttcga
     3841 cgtgcgcaag aagttcatcg agcgcaacct ggtggacacc cgctacgcca gccgcgtggt
     3901 gctgaacgcc ctgcaggagc acttccgcgc ccacaagatc gacaccaagg tgagcgtggt
     3961 gcgcggccag ttcaccagcc agctgcgccg ccactggggc atcgagaaga cccgcgacac
     4021 ctaccaccac cacgccgtgg acgccctgat cattgcggct tctagccagc tgaacctgtg
     4081 gaagaagcag aagaacaccc tggtgagcta cagcgaggac cagctgctgg acatcgagac
     4141 cggcgagctg atcagcgacg acgagtacaa ggagagcgtg ttcaaggccc cctaccagca
     4201 cttcgtggac accctgaaga gcaaggagtt cgaggacagc atcctgttca gctaccaggt
     4261 ggacagcaag ttcaaccgca agatcagcga cgccaccatc tacgccaccc gccaggccaa
     4321 ggtgggcaag gacaaggccg acgagaccta cgtgctgggc aagatcaagg acatctacac
     4381 ccaggacggc tacgacgcct tcatgaagat ctacaagaag gacaagagca agttcctgat
     4441 gtaccgccac gacccccaga ccttcgagaa ggtgatcgag cccatcctgg agaactaccc
     4501 caacaagcag atcaacgaga aaggcaagga ggtgccctgc aaccccttcc tgaagtacaa
     4561 ggaggagcac ggctacatcc gcaagtacag caagaagggc aacggccccg agatcaagag
     4621 cctgaagtac tacgacagca agctgggcaa ccacatcgac atcaccccca aggacagcaa
     4681 caacaaggtg gtgctgcaga gcgtgagccc ctggcgcgcc gacgtgtact tcaacaagac
     4741 caccggcaag tacgagatcc tggggctgaa gtacgccgat ctgcagtttg agaaaggcac
     4801 aggcacctac aagatcagcc aggagaagta caacgacatc aagaagaagg agggcgtgga
     4861 cagcgacagc gagttcaagt tcaccctgta caagaacgac cttctgctgg tgaaggacac
     4921 cgagaccaag gagcaacagc tgttccgctt cctgagccgc accatgccca agcagaagca
     4981 ctacgtggag ctgaagccct acgacaagca gaagttcgag ggcggcgagg ccctgatcaa
     5041 ggtgctgggc aacgtggcca acagcggcca gtgcaagaag ggcctgggca agagcaacat
     5101 cagcatctac aaggtgcgca ccgacgtgct gggcaaccag cacatcatca agaacgaggg
     5161 cgacaagccc aagttggact tcagcagggc tgaccccaag aagaagagga aggtgtgagc
     5221 ggccgcactc ctcaggtgca ggctgcctat cagaaggtgg tggctggtgt ggccaatgcc
     5281 ctggctcaca aataccactg agatcttttt ccctctgcca aaaattatgg ggacatcatg
     5341 aagccccttg agcatctgac ttctggctaa taaaggaaat ttattttcat tgcaatagtg
     5401 tgttggaatt ttttgtgtct ctcactcgga aggacatatg ggagggcaaa tcatttaaaa
     5461 catcagaatg agtatttggt ttagagtttg gcaacatatg cccatatgct ggctgccatg
     5521 aacaaaggtt ggctataaag aggtcatcag tatatgaaac agccccctgc tgtccattcc
     5581 ttattccata gaaaagcctt gacttgaggt tagatttttt ttatattttg ttttgtgtta
     5641 tttttttctt taacatccct aaaattttcc ttacatgttt tactagccag atttttcctc
     5701 ctctcctgac tactcccagt catagctgtc cctcttctct tatgaagatc cctcgacctg
     5761 cagcccaagc ttggcgtaat catggtcata gctgtttcct gtgtgaaatt gttatccgct
     5821 cacaattcca cacaacatac gagccggaag cataaagtgt aaagcctggg gtgcctaatg
     5881 agtgagctaa ctcacattaa ttgcgttgcg ctcactgccc gctttccagt cgggaaacct
     5941 gtcgtgccag cggatccgca tctcaattag tcagcaacca tagtcccgcc cctaactccg
     6001 cccatcccgc ccctaactcc gcccagttcc gcccattctc cgccccatgg ctgactaatt
     6061 ttttttattt atgcagaggc cgaggccgcc tcggcctctg agctattcca gaagtagtga
     6121 ggaggctttt ttggaggcct aggcttttgc aaaaagctaa cttgtttatt gcagcttata
     6181 atggttacaa ataaagcaat agcatcacaa atttcacaaa taaagcattt ttttcactgc
     6241 attctagttg tggtttgtcc aaactcatca atgtatctta tcatgtctgg atccgctgca
     6301 ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt attgggcgct cttccgcttc
     6361 ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat cagctcactc
     6421 aaaggcggta atacggttat ccacagaatc aggggataac gcaggaaaga acatgtgagc
     6481 aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag
     6541 gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc
     6601 gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt
     6661 tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct
     6721 ttctcaatgc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg
     6781 ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct
     6841 tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat
     6901 tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg
     6961 ctacactaga aggacagtat ttggtatctg cgctctgctg aagccagtta ccttcggaaa
     7021 aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt
     7081 ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc
     7141 tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt
     7201 atcaaaaagg atcttcacct agatcctttt aaattaaaaa tgaagtttta aatcaatcta
     7261 aagtatatat gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg aggcacctat
     7321 ctcagcgatc tgtctatttc gttcatccat agttgcctga ctccccgtcg tgtagataac
     7381 tacgatacgg gagggcttac catctggccc cagtgctgca atgataccgc gagacccacg
     7441 ctcaccggct ccagatttat cagcaataaa ccagccagcc ggaagggccg agcgcagaag
     7501 tggtcctgca actttatccg cctccatcca gtctattaat tgttgccggg aagctagagt
     7561 aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt
     7621 gtcacgctcg tcgtttggta tggcttcatt cagctccggt tcccaacgat caaggcgagt
     7681 tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt
     7741 cagaagtaag ttggccgcag tgttatcact catggttatg gcagcactgc ataattctct
     7801 tactgtcatg ccatccgtaa gatgcttttc tgtgactggt gagtactcaa ccaagtcatt
     7861 ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac gggataatac
     7921 cgcgccacat agcagaactt taaaagtgct catcattgga aaacgttctt cggggcgaaa
     7981 actctcaagg atcttaccgc tgttgagatc cagttcgatg taacccactc gtgcacccaa
     8041 ctgatcttca gcatctttta ctttcaccag cgtttctggg tgagcaaaaa caggaaggca
     8101 aaatgccgca aaaaagggaa taagggcgac acggaaatgt tgaatactca tactcttcct
     8161 ttttcaatat tattgaagca tttatcaggg ttattgtctc atgagcggat acatatttga
     8221 atgtatttag aaaaataaac aaataggggt tccgcgcaca tttccccgaa aagtgccacc
     8281 tg
//
