LOCUS       sequence-208001-       15728 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Arabidopsis transformation, expresses GA2ox2 with RCH1 promoter to 
            study expression pattern in roots.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 15728)
  AUTHORS   Li H, Torres-Garcia J, Latrasse D, Benhamed M, Schilderink S, Zhou 
            W, Kulikova O, Hirt H, Bisseling T
  TITLE     Plant-Specific Histone Deacetylases HDT1/2 Regulate GIBBERELLIN 
            2-OXIDASE2 Expression to Control Arabidopsis Root Meristem Cell 
            Number.
  JOURNAL   Plant Cell. 2017 Sep;29(9):2183-2196. doi: 10.1105/tpc.17.00366. 
            Epub 2017 Aug 30.
  PUBMED    28855334
REFERENCE   2  (bases 1 to 15728)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1105/tpc.17.00366"; journalName: "Plant Cell"; date: "2017-09"; 
            volume: "29"; issue: "9"; pages: "2183-2196"
COMMENT     Sequence Label: RCH1:GA2ox2-GFP
FEATURES             Location/Qualifiers
     source          1..15728
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    2167..2191
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(3372..3396)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             join(3404..3406,3407..3409,3410..4120)
                     /codon_start=1
                     /product="strongly enhanced green fluorescent protein 
                     (Kremers et al., 2007)"
                     /label=SGFP2
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITADKQKNGIK
                     ANFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(3449..3470)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(3710..3729)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     4057..4078
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     protein_bind    complement(4362..4382)
                     /gene="mutant version of attB"
                     /label=attB3
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     primer_bind     complement(4582..4603)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     4794..4813
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     terminator      complement(5095..5347)
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     promoter        7307..7486
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     CDS             7528..8079
                     /codon_start=1
                     /gene="Streptomyces hygroscopicus bar"
                     /product="phosphinothricin acetyltransferase"
                     /label=BlpR
                     /note="confers resistance to bialophos or phosphinothricin"
                     /translation="MSPERRPADIRRATEADMPAVCTIVNHYIETSTVNFRTEPQEPQE
                     WTDDLVRLRERYPWLVAEVDGEVAGIAYAGPWKARNAYDWTAESTVYVSPRHQRTGLGS
                     TLYTHLLKSLEAQGFKSVVAVIGLPNDPSVRMHEALGYAPRGMLRAAGFKHGNWHDVGF
                     WQLDFSLPVPPRPVLPVTEI"
     terminator      8099..8351
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     misc_feature    8444..8468
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             8989..9780
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MGEAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      10026..10614
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     10515..10534
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     10768..10785
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    10800..10940
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(10866..10885)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     11026..11048
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     rep_origin      11284..11478
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     CDS             complement(11544..12617)
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     CDS             complement(13046..13675)
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     misc_feature    15006..15030
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     primer_bind     15097..15113
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     15097..15113
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     protein_bind    15133..15153
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
ORIGIN
        1 tttaaattaa gctgacctgc aaaacgagaa gcgggaaaga gtgaatgcca gcggagtgat
       61 caacttcggg aataagctga cggagattaa gggtaggagc aaaatcacca aaatcatcga
      121 taacagaagg agtatcagcc acaacgaaga gaacaccagc accgttacaa tcgatctcaa
      181 tacgaccatc atcgtctctc ttcaagcgac cagccatagg gtaaaaaggg acaagggctt
      241 tggaaagagc ttccttcatg acctgagggt caaagaaatt ggaagcgccg gtgggtctgt
      301 agaagtagac actaggggta tggaatctgg ggatgacaag gtcgacgttg gagttccaaa
      361 gattagtgat tggtgtctcg gtggcaggcc ggaccatggt ggaatctctg atgttaattt
      421 tcatattttt aagaaagatc aaacgaacaa acaaattttg ttttgagttt gatctgagaa
      481 attgtgtgag aagcgaggag gggtagatat aagtgggtgt gaacaaaaaa ggaaaggtcg
      541 taatccttca cctaccgccc cacgaccccg cctactccat ctgtcgacca actctctcag
      601 aaggtttatc attggtccat ctcatcactc ggtcctactt ttctatccgg ggttggtaaa
      661 cccgttccct ctgtcctaag gcccaaataa tactaatggg cttaaatagt ataaagttcg
      721 aatgaagaag aaggaaaaaa aattgacaag ttgattgtta tagctaccgt acaagttagt
      781 caatcgaatg ccaaagtttg gcttaactta actcattacc acgtagcaga gcataacaac
      841 aaccaccaat aacatctaac ggcgtaaact cacgtgttaa gagaattacg tcagaatcca
      901 agaaagtctt tttttcagta caccacaaat aaattatgtt gaatttatat ccgaactata
      961 ctatattata tgaaaacata cctcaattag tcataaattt cacatttcat tttaatttat
     1021 taactaatgg ttgttgttgc ggatagcttt tctttgcttt ctgttctaaa attatttctt
     1081 agccattatt tgatgatcca acttggtttg aggatctaat aaaaattcaa tttctttgaa
     1141 gcattcattg gcttaatgat ccaaaccttt tgtcctccca ctctctaaga aaataaagca
     1201 aaagcaaaac taaaagaaaa gcaactagta ccgtgggttg gtcaatggta acccatgcta
     1261 ccaaatacca atcaaagctt aaatgacaat ttagtacctt gggttggtca tgatttagag
     1321 cggaacaaat ataccataca tcaaacgagg atatacagag aaaattcatg gaagtatgga
     1381 atttagagga caatttctct tctgggctac aacggaccgg cccattcgct catttaccca
     1441 gaggtatcga gtttgtggac ttttgatgcc gctagagact attggcatcg gattgaaaaa
     1501 aatgtttact tcgttgttaa caattttctg aatgcaatat tttccttgtc atgaatattt
     1561 aaacttgtta ttactttctt ttagcttagg tgtggacaat tatggagttt acttcaaacg
     1621 aggaagaatc ttaaacgctc ggttcaggtc tcgaaaacaa accaactcac aatcctgact
     1681 taattgagga aaacaatgca aaaccacatg catgcttcca tatttctatc ataatcttat
     1741 aagaaaaaac actactaagt gaaatgattc tgtatatata taaccaatgc cttttgtttt
     1801 gtgatatttt atgtatatat aactattgac ttttgtcatc tatggatagt gtctcgggct
     1861 cttggcaaac atatttcaaa gaaaagttaa tgactgtaat taattaatct gaagctagaa
     1921 acagaacccc gaggtaaaag aaaaagacag agcacatgaa gtttagtact tttatatatt
     1981 taatatatca ttctttctta ttgcttatct ctaaagcaaa aacttcccta aaccctaagc
     2041 caaaggactc agatcgatgc agaaccaaga aggcttgttt tggatttgag agccaaatgc
     2101 aaagaaaaaa actctggatc gacgtaaagg gtgggcgcgc caaagggcgc gcctaacccg
     2161 ggtaccacaa gtttgtacaa aaaagcaggc tccgcggccg cccccttcac cagttcctaa
     2221 atcataacta caaaaacttt tccttcttct tcctcaacct ttgcttcaat cttcaacaac
     2281 tttcttttta taaagatttt gcaagttaag tgtaaaccta caaaaaccaa acatggtggt
     2341 tttgccacag ccagtcactt tagataacca catctcccta atccccacat acaaaccggt
     2401 tccggttctc acttcccatt caatccccgt cgtcaaccta gccgatccgg aagcgaaaac
     2461 ccgaatcgta aaagcctgcg aggagttcgg gttcttcaag gtcgtaaacc acggagtccg
     2521 acccgaactc atgactcggt tagagcagga ggctattggc ttcttcggct tgcctcagtc
     2581 tcttaaaaac cgggccggtc cacctgaacc gtacggttat ggtaataaac ggattggacc
     2641 aaacggtgac gttggttgga ttgagtatct cctcctcaat gctaatcctc agctctcctc
     2701 tcctaaaacc tccgccgttt tccgtcaaac ccctcaaatt ttccgtgagt cggtggagga
     2761 gtacatgaag gagattaagg aagtgtcgta caaggtgttg gagatggttg ccgaagaact
     2821 agggatagag ccaagggaca ctctgagtaa aatgctgaga gatgagaaga gtgactcgtg
     2881 cctgagacta aaccattatc cggcggcgga ggaagaggcg gagaagatgg tgaaggtggg
     2941 gtttggggaa cacacagacc cacagataat ctcagtgcta agatctaata acacggcggg
     3001 tcttcaaatc tgtgtgaaag atggaagttg ggtcgctgtc cctcctgatc actcttcttt
     3061 cttcattaat gttggagatg ctcttcaggt tatgactaac gggaggttca agagtgttaa
     3121 acacagggtc ttagccgata caaggagatc gaggatttca atgatatatt tcggcggacc
     3181 gccattgagc cagaagatcg caccattgcc atgccttgtc cctgagcaag atgattggct
     3241 ttacaaagaa ttcacttggt ctcaatacaa atcttctgct tacaagtcta agcttggtga
     3301 ttatagactt ggtctctttg agaaacaacc tcttctcaat cataaaaccc ttgtaaaggg
     3361 tgggcgcgcc gacccagctt tcttgtacaa agtggggcgc gccatggtga gcaagggcga
     3421 ggagctgttc accggggtgg tgcccatcct ggtcgagctg gacggcgacg taaacggcca
     3481 caagttcagc gtgtccggcg agggcgaggg cgatgccacc tacggcaagc tgaccctgaa
     3541 gttcatctgc accaccggca agctgcccgt gccctggccc accctcgtga ccaccctgac
     3601 ctacggcgtg cagtgcttcg cccgctaccc cgaccacatg aagcagcacg acttcttcaa
     3661 gtccgccatg cccgaaggct acgtccagga gcgcaccatc ttcttcaagg acgacggcaa
     3721 ctacaagacc cgcgccgagg tgaagttcga gggcgacacc ctggtgaacc gcatcgagct
     3781 gaagggcatc gacttcaagg aggacggcaa catcctgggg cacaagctgg agtacaacta
     3841 caacagccac aacgtctata tcaccgccga caagcagaag aacggcatca aggccaactt
     3901 caagatccgc cacaacatcg aggacggcgg cgtgcagctc gccgaccact accagcagaa
     3961 cacccccatc ggcgacggcc ccgtgctgct gcccgacaac cactacctga gcacccagtc
     4021 caagctgagc aaagacccca acgagaagcg cgatcacatg gtcctgctgg agttcgtgac
     4081 cgccgccggg atcactctcg gcatggacga gctgtacaag ggtacctaac cgcggccatg
     4141 ctagagtccg caaaaatcac cagtctctct ctacaaatct atctctctct atttttctcc
     4201 agaataatgt gtgagtagtt cccagataag ggaattaggg ttcttatagg gtttcgctca
     4261 tgtgttgagc atataagaaa cccttagtat gtatttgtat ttgtaaaata cttctatcaa
     4321 taaaatttct aattcctaaa accaaaatcc agtgatccgg acaactttat tatacatagt
     4381 tgataattca ctggccgtcg tgacgtcggg cccatcgatg gcgcgctgat tgcccttcac
     4441 cgcctggccc tgagagagtt gcagcaagcg gtccacgctg gtttgcccca gcaggcgaaa
     4501 atcctgtttg atggtggttc cgaaatcggc aaaatccctt ataaatcaaa agaatagccc
     4561 gagatagggt tgagtgttgt tccagtttgg aacaagagtc cactattaaa gaacgtggac
     4621 tccaacgtca aagggcgaaa aaccgtctat cagggcgatg gcccactacg tgaaccatca
     4681 cccaaatcaa gttttttggg gtcgaggtgc cgtaaagcac taaatcggaa ccctaaaggg
     4741 agcccccgat ttagagcttg acggggaaag ccggcgaacg tggcgagaaa ggaagggaag
     4801 aaagcgaaag gagcgggcgc cattcaggcc gatagcagca ccgtaatcag tagcgacaga
     4861 atcaagtttg cctttagcgt cagactgtag cgcgttttca tcggcatttt cggtcatagc
     4921 ccccttatta gcgtttgcca tcttttcata atcaaaatca ccggaaccag agccaccacc
     4981 ggaaccgcct ccctcagagc cgccaccctc agaaccgcca ccctcagagc caccaccctc
     5041 agagccgcca ccagaaccac caccagagcc gccgccagca ttgacaggag gcccgatcta
     5101 gtaacataga tgacaccgcg cgcgataatt tatcctagtt tgcgcgctat attttgtttt
     5161 ctatcgcgta ttaaatgtat aattgcggga ctctaatcat aaaaacccat ctcataaata
     5221 acgtcatgca ttacatgtta attattacat gcttaacgta attcaacaga aattatatga
     5281 taatcatcgc aagaccggca acaggattca atcttaagaa actttattgc caaatgtttg
     5341 aacgatcggg gatccgcttt acttgtacat aaggaacaga tggtggcgtc cctcggttct
     5401 ttcatactgc tcaacgattg tatagtcttc gttgtggctt gttatatcca gtttggagtc
     5461 aacatagtag taccctggta gctgcacagg cttctttgcc atgtaaatac ttttgaattc
     5521 aactaggtaa tgaccaccgt ctttcagctt cagagcctta tgaatctctc ctttcaacac
     5581 gccatcacga ggatacaaac gctcagtgct ggcttcccag cccattgtct tcttttgcat
     5641 aacaggtcca tcggaaggaa agttcacgcc aatgaacttg accttgtaga tgaaacagcc
     5701 atcctgcaaa ctggaatcct gggttacagt aacgacgcca ccgtcttcaa agttcatgac
     5761 cctttcccat ttaaatcctt caggaaatga cagcttttta tagtctggta tgtcggcagg
     5821 gtgcttgaca tataccttgc ttccatactg aaattgtggt gacaaaatat cccaagcaaa
     5881 tggcaaaggt ccccccttgg ttaccttaag ctttacggta ttgtggcctt cgtatggcct
     5941 cccctctcct tcgccttcta tttcaaactc gtgcccattg accgttcctt ccatgcgaac
     6001 cttaaacctc atgaactcct tgataacatt cttggaagac cccatggggt atgtttttaa
     6061 tcttgtttgt attgatgagt tttggtttga gtaaagagtg aagcggatga gttaatttat
     6121 agactataaa ggagatttgc atggcgatca cgtgtaataa tgcatgcacg catgtgattg
     6181 tatgtgtgtg ctgtgagaga gaagctctta ggtgtttgaa gggagtgaca agtggcgaag
     6241 aaaaacaatt ctccgcggct gcatgctatg tgtaacgtgt agctaatgtt ctggcatggc
     6301 atcttatgaa cgattctttt taaaaacaag gtaaaaactt aacttcataa aattaaaaaa
     6361 aagagcgttt actaagttgg tttaaaaggg gatgagagtc tataaatttt ggaggtagtg
     6421 ccgttgggaa tataaattgg gagcttaatc agaattatag aagttaaagt tgatttagtc
     6481 acggtcaata taaattggga atttgagtca aaatcttcca aattcggaat ccgtcttgtt
     6541 acacccggtg gataggagcc gaacggtttg aaaatacttg aaatgtggat gcaggtgcag
     6601 gctggtttaa ttttatgttg aatggataca tgtcaatcga atttgagtta taggtacaca
     6661 ttttactctg atactaaaat gtaacatttg tctcaagaat gggtaggtca tccttactcg
     6721 acaacggtat gccacttcac ttgaacgtat atctcattca acccaactct aacacacatt
     6781 tcatacccat gtctaaaata aagaaaattg aagaaaaacg ctcaatagcg tcaatcctta
     6841 caaatataat gtacatggca acctcgcatg aaaatagtaa taaagaggag tgaataatat
     6901 aaagtagtca ctaattggat gacaaagcaa atggaaccat ccgatagatg ttttgagaac
     6961 aagaggtaca tttgtatata tgcattggtt gatacatttc tgccatctga attgtttctt
     7021 cctcaaagaa gaagcattca gccaatagtg aaatttaaaa tgttggaaaa atgaggatgc
     7081 agtcagaaag tccagaatat aagtggtaac tggtaactga atacttgcct cagaagcaag
     7141 ataagaaaca taagtcttta aaggaatcaa tcaccgatga agagagctcc gaatcgaatt
     7201 cgagctcggt accgcgaatt atcgatcatg agcggagaat taagggagtc acgttatgac
     7261 ccccgccgat gacgcgggac aagccgtttt acgtttggaa ctgacagaac cgcaacgttg
     7321 aaggagccac tgagccgcgg gtttctggag tttaatgagc taagcacata cgtcagaaac
     7381 cattattgcg cgttcaaaag tcgcctaagg tcactatcag ctagcaaata tttcttgtca
     7441 aaaatgctcc actgacgttc cataaattcc cctcggtatc caattagagt ctcatattca
     7501 ctctcaactc gatcgagggg atctaccatg agcccagaac gacgcccggc cgacatccgc
     7561 cgtgccaccg aggcggacat gccggcggtc tgcaccatcg tcaaccacta catcgagaca
     7621 agcacggtca acttccgtac cgagccgcag gaaccgcagg agtggacgga cgacctcgtc
     7681 cgtctgcggg agcgctatcc ctggctcgtc gccgaggtgg acggcgaggt cgccggcatc
     7741 gcctacgcgg gtccctggaa ggcacgcaac gcctacgact ggacggccga gtcgaccgtg
     7801 tacgtctccc cccgccacca gcggacggga ctgggctcca cgctctacac ccacctgctg
     7861 aagtccctgg aggcacaggg cttcaagagc gtggtcgctg tcatcgggct gcccaacgac
     7921 ccgagcgtgc gcatgcacga ggcgctcgga tatgcccccc gcggcatgct gcgggcggcc
     7981 ggcttcaagc acgggaactg gcatgacgtg ggtttctggc agctggactt cagcctgccg
     8041 gtgccgcccc gtccggtcct gcccgtcacc gaaatctgat gacccctaga gtcaagcaga
     8101 tcgttcaaac atttggcaat aaagtttctt aagattgaat cctgttgccg gtcttgcgat
     8161 gattatcata taatttctgt tgaattacgt taagcatgta ataattaaca tgtaatgcat
     8221 gacgttattt atgagatggg tttttatgat tagagtcccg caattataca tttaatacgc
     8281 gatagaaaac aaaatatagc gcgcaaacta ggataaatta tcgcgcgcgg tgtcatctat
     8341 gttactagat cgaccggcat gcaagctgat aattcaattc ggcgttaatt cagtacatta
     8401 aaaacgtccg caatgtgtta ttaagttgtc taagcgtcaa tttgtttaca ccacaatata
     8461 tcctgccacc agccagccaa cagctccccg accggcagct cggcacaaaa tcaccactcg
     8521 atacaggcag cccatcagtc cgggacggcg tcagcgggag agccgttgta aggcggcaga
     8581 ctttgctcat gttaccgatg ctattcggaa gaacggcaac taagctgccg ggtttgaaac
     8641 acggatgatc tcgcggaggg tagcatgttg attgtaacga tgacagagcg ttgctgcctg
     8701 tgatcaattc gggcacgaac ccagtggaca taagcctgtt cggttcgtaa gctgtaatgc
     8761 aagtagcgta tgcgctcacg caactggtcc agaaccttga ccgaacgcag cggtggtaac
     8821 ggcgcagtgg cggttttcat ggcttgttat gactgttttt ttggggtaca gtctatgcct
     8881 cgggcatcca agcagcaagc gcgttacgcc gtgggtcgat gtttgatgtt atggagcagc
     8941 aacgatgtta cgcagcaggg cagtcgccct aaaacaaagt taaacatcat gggggaagcg
     9001 gtgatcgccg aagtatcgac tcaactatca gaggtagttg gcgtcatcga gcgccatctc
     9061 gaaccgacgt tgctggccgt acatttgtac ggctccgcag tggatggcgg cctgaagcca
     9121 cacagtgata ttgatttgct ggttacggtg accgtaaggc ttgatgaaac aacgcggcga
     9181 gctttgatca acgacctttt ggaaacttcg gcttcccctg gagagagcga gattctccgc
     9241 gctgtagaag tcaccattgt tgtgcacgac gacatcattc cgtggcgtta tccagctaag
     9301 cgcgaactgc aatttggaga atggcagcgc aatgacattc ttgcaggtat cttcgagcca
     9361 gccacgatcg acattgatct ggctatcttg ctgacaaaag caagagaaca tagcgttgcc
     9421 ttggtaggtc cagcggcgga ggaactcttt gatccggttc ctgaacagga tctatttgag
     9481 gcgctaaatg aaaccttaac gctatggaac tcgccgcccg actgggctgg cgatgagcga
     9541 aatgtagtgc ttacgttgtc ccgcatttgg tacagcgcag taaccggcaa aatcgcgccg
     9601 aaggatgtcg ctgccgactg ggcaatggag cgcctgccgg cccagtatca gcccgtcata
     9661 cttgaagcta gacaggctta tcttggacaa gaagaagatc gcttggcctc gcgcgcagat
     9721 cagttggaag aatttgtcca ctacgtgaaa ggcgagatca ccaaggtagt cggcaaataa
     9781 tgtctagcta gaaattcgtt caagccgacg ccgcttcgcg gcgcggctta actcaagcgt
     9841 tagatgcact aagcacataa ttgctcacag ccaaactatc aggtcaagtc tgcttttatt
     9901 atttttaagc gtgcataata agccctacac aaattgggag atatatcatg catgaccaaa
     9961 atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga
    10021 tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg
    10081 ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact
    10141 ggcttcagca gagcgcagat accaaatact gtccttctag tgtagccgta gttaggccac
    10201 cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg
    10261 gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg
    10321 gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga
    10381 acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc
    10441 gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg
    10501 agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc
    10561 tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc
    10621 agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt
    10681 cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc
    10741 gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc
    10801 ctgatgcggt attttctcct tacgcatctg tgcggtattt cacaccgcat atggtgcact
    10861 ctcagtacaa tctgctctga tgccgcatag ttaagccagt atacactccg ctatcgctac
    10921 gtgactgggt catggctgcg ccccgacacc cgccaacacc cgctgacgcg ccctgacggg
    10981 cttgtctgct cccggcatcc gcttacagac aagctgtgac cgtctccggg agctgcatgt
    11041 gtcagaggtt ttcaccgtca tcaccgaaac gcgcgaggca gggtgccttg atgtgggcgc
    11101 cggcggtcga gtggcgacgg cgcggcttgt ccgcgccctg gtagattgcc tggccgtagg
    11161 ccagccattt ttgagcggcc agcggccgcg ataggccgac gcgaagcggc ggggcgtagg
    11221 gagcgcagcg accgaagggt aggcgctttt tgcagctctt cggctgtgcg ctggccagac
    11281 agttatgcac aggccaggcg ggttttaaga gttttaataa gttttaaaga gttttaggcg
    11341 gaaaaatcgc cttttttctc ttttatatca gtcacttaca tgtgtgaccg gttcccaatg
    11401 tacggctttg ggttcccaat gtacgggttc cggttcccaa tgtacggctt tgggttccca
    11461 atgtacgtgc tatccacagg aaagagacct tttcgacctt tttcccctgc tagggcaatt
    11521 tgccctagca tctgctccgt acattaggaa ccggcggatg cttcgccctc gatcaggttg
    11581 cggtagcgca tgactaggat cgggccagcc tgccccgcct cctccttcaa atcgtactcc
    11641 ggcaggtcat ttgacccgat cagcttgcgc acggtgaaac agaacttctt gaactctccg
    11701 gcgctgccac tgcgttcgta gatcgtcttg aacaaccatc tggcttctgc cttgcctgcg
    11761 gcgcggcgtg ccaggcggta gagaaaacgg ccgatgccgg gatcgatcaa aaagtaatcg
    11821 gggtgaaccg tcagcacgtc cgggttcttg ccttctgtga tctcgcggta catccaatca
    11881 gctagctcga tctcgatgta ctccggccgc ccggtttcgc tctttacgat cttgtagcgg
    11941 ctaatcaagg cttcaccctc ggataccgtc accaggcggc cgttcttggc cttcttcgta
    12001 cgctgcatgg caacgtgcgt ggtgtttaac cgaatgcagg tttctaccag gtcgtctttc
    12061 tgctttccgc catcggctcg ccggcagaac ttgagtacgt ccgcaacgtg tggacggaac
    12121 acgcggccgg gcttgtctcc cttcccttcc cggtatcggt tcatggattc ggttagatgg
    12181 gaaaccgcca tcagtaccag gtcgtaatcc cacacactgg ccatgccggc cggccctgcg
    12241 gaaacctcta cgtgcccgtc tggaagctcg tagcggatca cctcgccagc tcgtcggtca
    12301 cgcttcgaca gacggaaaac ggccacgtcc atgatgctgc gactatcgcg ggtgcccacg
    12361 tcatagagca tcggaacgaa aaaatctggt tgctcgtcgc ccttgggcgg cttcctaatc
    12421 gacggcgcac cggctgccgg cggttgccgg gattctttgc ggattcgatc agcggccgct
    12481 tgccacgatt caccggggcg tgcttctgcc tcgatgcgtt gccgctgggc ggcctgcgcg
    12541 gccttcaact tctccaccag gtcatcaccc agcgccgcgc cgatttgtac cgggccggat
    12601 ggtttgcgac cgctcacgcc gattcctcgg gcttgggggt tccagtgcca ttgcagggcc
    12661 ggcagacaac ccagccgctt acgcctggcc aaccgcccgt tcctccacac atggggcatt
    12721 ccacggcgtc ggtgcctggt tgttcttgat tttccatgcc gcctccttta gccgctaaaa
    12781 ttcatctact catttattca tttgctcatt tactctggta gctgcgcgat gtattcagat
    12841 agcagctcgg taatggtctt gccttggcgt accgcgtaca tcttcagctt ggtgtgatcc
    12901 tccgccggca actgaaagtt gacccgcttc atggctggcg tgtctgccag gctggccaac
    12961 gttgcagcct tgctgctgcg tgcgctcgga cggccggcac ttagcgtgtt tgtgcttttg
    13021 ctcattttct ctttacctca ttaactcaaa tgagttttga tttaatttca gcggccagcg
    13081 cctggacctc gcgggcagcg tcgccctcgg gttctgattc aagaacggtt gtgccggcgg
    13141 cggcagtgcc tgggtagctc acgcgctgcg tgatacggga ctcaagaatg ggcagctcgt
    13201 acccggccag cgcctcggca acctcaccgc cgatgcgcgt gcctttgatc gcccgcgaca
    13261 cgacaaaggc cgcttgtagc cttccatccg tgacctcaat gcgctgctta accagctcca
    13321 ccaggtcggc ggtggcccat atgtcgtaag ggcttggctg caccggaatc agcacgaagt
    13381 cggctgcctt gatcgcggac acagccaagt ccgccgcctg gggcgctccg tcgatcacta
    13441 cgaagtcgcg ccggccgatg gccttcacgt cgcggtcaat cgtcgggcgg tcgatgccga
    13501 caacggttag cggttgatct tcccgcacgg ccgcccaatc gcgggcactg ccctggggat
    13561 cggaatcgac taacagaaca tcggccccgg cgagttgcag ggcgcgggct agatgggttg
    13621 cgatggtcgt cttgcctgac ccgcctttct ggttaagtac agcgataacc ttcatgcgtt
    13681 ccccttgcgt atttgtttat ttactcatcg catcatatac gcagcgaccg catgacgcaa
    13741 gctgttttac tcaaatacac atcacctttt tagacggcgg cgctcggttt cttcagcggc
    13801 caagctggcc ggccaggccg ccagcttggc atcagacaaa ccggccagga tttcatgcag
    13861 ccgcacggtt gagacgtgcg cgggcggctc gaacacgtac ccggccgcga tcatctccgc
    13921 ctcgatctct tcggtaatga aaaacggttc gtcctggccg tcctggtgcg gtttcatgct
    13981 tgttcctctt ggcgttcatt ctcggcggcc gccagggcgt cggcctcggt caatgcgtcc
    14041 tcacggaagg caccgcgccg cctggcctcg gtgggcgtca cttcctcgct gcgctcaagt
    14101 gcgcggtaca gggtcgagcg atgcacgcca agcagtgcag ccgcctcttt cacggtgcgg
    14161 ccttcctggt cgatcagctc gcgggcgtgc gcgatctgtg ccggggtgag ggtagggcgg
    14221 gggccaaact tcacgcctcg ggccttggcg gcctcgcgcc cgctccgggt gcggtcgatg
    14281 attagggaac gctcgaactc ggcaatgccg gcgaacacgg tcaacaccat gcggccggcc
    14341 ggcgtggtgg tgtcggccca cggctctgcc aggctacgca ggcccgcgcc ggcctcctgg
    14401 atgcgctcgg caatgtccag taggtcgcgg gtgctgcggg ccaggcggtc tagcctggtc
    14461 actgtcacaa cgtcgccagg gcgtaggtgg tcaagcatcc tggccagctc cgggcggtcg
    14521 cgcctggtgc cggtgatctt ctcggaaaac agcttggtgc agccggccgc gtgcagttcg
    14581 gcccgttggt tggtcaagtc ctggtcgtcg gtgctgacgc gggcatagcc cagcaggcca
    14641 gcggcggcgc tcttgttcat ggcgtaatgt ctccggttct agtcgcaagt attctacttt
    14701 atgcgactaa aacacgcgac aagaaaacgc caggaaaagg gcagggcggc agcctgtcgc
    14761 gtaacttagg acttgtgcga catgtcgttt tcagaagacg gctgcactga acgtcagaag
    14821 ccgactgcac tatagcagcg gaggggttgg atcaaagtac tttaaagtac tttaaagtac
    14881 tttaaagtac tttgatcccg aggggaaccc tgtggttggc atgcacatac aaatggacga
    14941 acggataaac cttttcacgc ccttttaaat atccgattat tctaataaac gctcttttct
    15001 cttaggttta cccgccaata tatcctgtca aacactgata gtttaaactg aaggcgggaa
    15061 acgacaatct gatccaagct caagctaagc ttcacacagg aaacagctat gaccatgatt
    15121 acgccaagct atcaactttg tatagaaaag ttggccgcgg cccatcagtt gcaatgtaca
    15181 gtttcgtctc ttggtcgttt ggaagccctc gcgcctttcc cactgatctc cacacatgcc
    15241 ctgccaacat ctcgtatgag ctgtagctga cagtgttccc atcctccttg gatttcgcct
    15301 taagagcaac aagctggtct cgtgttaatt tgaatataga gacagtggta ttctcaggtc
    15361 ctgatttaga cggatcaaga ggtatcttca tacttggtgc aggctgatat tcaacatgat
    15421 gaaaagcagg ctgtggcggg tccctagctc ggaggagtgt tcgatcaatg aaaggtggaa
    15481 tggttaggtc aagaccacga gccatatcag accatgtgtt gataaaatga agaccagaga
    15541 aaccatctgc cgcgtgatgt tgcatcccaa ccccaagtga agctccccca catttaaaga
    15601 aagtcaccta tatcatcaaa caatatcatt acataactaa aaacagaaca tacatcatca
    15661 aagaatatca ttacagaact aaaaaacaga acaacacaag cattcaataa attaaaaaag
    15721 ggaaagac
//
