LOCUS       sequence-208002-        8715 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  A single vector mammalian expression system containing a CAG 
            promoter with Cas9 from Streptococcus thermophilus CRISPR1 (St1Cas9 
            LMD-9) and its U6-driven sgRNA..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8715)
  AUTHORS   Agudelo D, Carter S, Velimirovic M, Duringer A, Rivest JF, Levesque 
            S, Loehr J, Mouchiroud M, Cyr D, Waters PJ, Laplante M, Moineau S, 
            Goulet A, Doyon Y
  TITLE     Versatile and robust genome editing with Streptococcus thermophilus 
            CRISPR1-Cas9.
  JOURNAL   Genome Res. 2020 Jan;30(1):107-117. doi: 10.1101/gr.255414.119. Epub
            2020 Jan 3.
  PUBMED    31900288
REFERENCE   2  (bases 1 to 8715)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1101/gr.255414.119"; journalName: "Genome Res"; date: "2020-01";
            volume: "30"; issue: "1"; pages: "107-117"
COMMENT     Sequence Label: U6_sgRNA_CAG_hSt1Cas9_LMD9
FEATURES             Location/Qualifiers
     source          1..8715
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        93..341
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     primer_bind     93..113
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     primer_bind     264..283
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     enhancer        513..816
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        819..1094
                     /label=chicken beta-actin promoter
     intron          1095..2103
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     2027..2049
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     2111..2130
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     regulatory      2196..2205
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             2211..2231
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             2256..5615
                     /codon_start=1
                     /product="Cas9 endonuclease from the Streptococcus 
                     thermophilus Type II CRISPR/Cas system (Esvelt et al., 
                     2013)"
                     /label=St1Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="SDLVLGLDIGIGSVGVGILNKVTGEIIHKNSRIFPAAQAENNLVR
                     RTNRQGRRLARRKKHRRVRLNRLFEESGLITDFTKISINLNPYQLRVKGLTDELSNEEL
                     FIALKNMVKHRGISYLDDASDDGNSSVGDYAQIVKENSKQLETKTPGQIQLERYQTYGQ
                     LRGDFTVEKDGKKHRLINVFPTSAYRSEALRILQTQQEFNPQITDEFINRYLEILTGKR
                     KYYHGPGNEKSRTDYGRYRTSGETLDNIFGILIGKCTFYPDEFRAAKASYTAQEFNLLN
                     DLNNLTVPTETKKLSKEQKNQIINYVKNEKAMGPAKLFKYIAKLLSCDVADIKGYRIDK
                     SGKAEIHTFEAYRKMKTLETLDIEQMDRETLDKLAYVLTLNTEREGIQEALEHEFADGS
                     FSQKQVDELVQFRKANSSIFGKGWHNFSVKLMMELIPELYETSEEQMTILTRLGKQKTT
                     SSSNKTKYIDEKLLTEEIYNPVVAKSVRQAIKIVNAAIKEYGDFDNIVIEMARETNEDD
                     EKKAIQKIQKANKDEKDAAMLKAANQYNGKAELPHSVFHGHKQLATKIRLWHQQGERCL
                     YTGKTISIHDLINNSNQFEVDHILPLSITFDDSLANKVLVYATANQEKGQRTPYQALDS
                     MDDAWSFRELKAFVRESKTLSNKKKEYLLTEEDISKFDVRKKFIERNLVDTRYASRVVL
                     NALQEHFRAHKIDTKVSVVRGQFTSQLRRHWGIEKTRDTYHHHAVDALIIAASSQLNLW
                     KKQKNTLVSYSEDQLLDIETGELISDDEYKESVFKAPYQHFVDTLKSKEFEDSILFSYQ
                     VDSKFNRKISDATIYATRQAKVGKDKADETYVLGKIKDIYTQDGYDAFMKIYKKDKSKF
                     LMYRHDPQTFEKVIEPILENYPNKQINEKGKEVPCNPFLKYKEEHGYIRKYSKKGNGPE
                     IKSLKYYDSKLGNHIDITPKDSNNKVVLQSVSPWRADVYFNKTTGKYEILGLKYADLQF
                     EKGTGTYKISQEKYNDIKKKEGVDSDSEFKFTLYKNDLLLVKDTETKEQQLFRFLSRTM
                     PKQKHYVELKPYDKQKFEGGEALIKVLGNVANSGQCKKGLGKSNISIYKVRTDVLGNQH
                     IIKNEGDKPKLDF"
     CDS             5628..5648
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     primer_bind     complement(5737..5756)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    5802..5857
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(5856..5875)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(6218..6234)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6218..6234)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6231..6253)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6242..6258
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6266..6272,6273..6290,6291..6296))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6311..6332
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        6391..6586
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      6437..6572
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     polyA_signal    6592..6726
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6629..6648)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6683..6702
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(6794..6811)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6965..7553)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7045..7064)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7724..8515,8516..8584))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8347..8366
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8585..8689)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gtcgacattg attattgact agctgtacaa aaaagcaggc tttaaaggaa ccaattcagt
       61 cgactggatc cggtaccaag gtcgggcagg aagagggcct atttcccatg attccttcat
      121 atttgcatat acgatacaag gctgttagag agataattag aattaatttg actgtaaaca
      181 caaagatatt agtacaaaat acgtgacgta gaaagtaata atttcttggg tagtttgcag
      241 ttttaaaatt atgttttaaa atggactatc atatgcttac cgtaacttga aagtatttcg
      301 atttcttggc tttatatatc ttgtggaaag gacgaaacac ccgagacgat taatgcgtct
      361 cggtctttgt actctggtac cagaagctac aaagataagg cttcatgccg aaatcaacac
      421 cctgtcattt tatggcaggg tgtttttttt actagttatt aatagtaatc aattacgggg
      481 tcattagttc atagcccata tatggagttc cgcgttacat aacttacggt aaatggcccg
      541 cctggctgac cgcccaacga cccccgccca ttgacgtcaa taatgacgta tgttcccata
      601 gtaacgccaa tagggacttt ccattgacgt caatgggtgg actatttacg gtaaactgcc
      661 cacttggcag tacatcaagt gtatcatatg ccaagtacgc cccctattga cgtcaatgac
      721 ggtaaatggc ccgcctggca ttatgcccag tacatgacct tatgggactt tcctacttgg
      781 cagtacatct acgtattagt catcgctatt accatgggtc gaggtgagcc ccacgttctg
      841 cttcactctc cccatctccc ccccctcccc acccccaatt ttgtatttat ttatttttta
      901 attattttgt gcagcgatgg gggcgggggg ggggggggcg cgcgccaggc ggggcggggc
      961 ggggcgaggg gcggggcggg gcgaggcgga gaggtgcggc ggcagccaat cagagcggcg
     1021 cgctccgaaa gtttcctttt atggcgaggc ggcggcggcg gcggccctat aaaaagcgaa
     1081 gcgcgcggcg ggcgggagtc gctgcgttgc cttcgccccg tgccccgctc cgcgccgcct
     1141 cgcgccgccc gccccggctc tgactgaccg cgttactccc acaggtgagc gggcgggacg
     1201 gcccttctcc tccgggctgt aattagcgct tggtttaatg acggctcgtt tcttttctgt
     1261 ggctgcgtga aagccttaaa gggctccggg agggcccttt gtgcgggggg gagcggctcg
     1321 gggggtgcgt gcgtgtgtgt gtgcgtgggg agcgccgcgt gcggcccgcg ctgcccggcg
     1381 gctgtgagcg ctgcgggcgc ggcgcggggc tttgtgcgct ccgcgtgtgc gcgaggggag
     1441 cgcggccggg ggcggtgccc cgcggtgcgg gggggctgcg aggggaacaa aggctgcgtg
     1501 cggggtgtgt gcgtgggggg gtgagcaggg ggtgtgggcg cggcggtcgg gctgtaaccc
     1561 ccccctgcac ccccctcccc gagttgctga gcacggcccg gcttcgggtg cggggctccg
     1621 tgcggggcgt ggcgcggggc tcgccgtgcc gggcgggggg tggcggcagg tgggggtgcc
     1681 gggcggggcg gggccgcctc gggccgggga gggctcgggg gaggggcgcg gcggccccgg
     1741 agcgccggcg gctgtcgagg cgcggcgagc cgcagccatt gccttttatg gtaatcgtgc
     1801 gagagggcgc agggacttcc tttgtcccaa atctggcgga gccgaaatct gggaggcgcc
     1861 gccgcacccc ctctagcggg cgcgggcgaa gcggtgcggc gccggcagga aggaaatggg
     1921 cggggagggc cttcgtgcgt cgccgcgccg ccgtcccctt ctccatctcc agcctcgggg
     1981 ctgccgcagg gggacggctg ccttcggggg ggacggggca gggcggggtt cggcttctgg
     2041 cgtgtgaccg gcggctctag agcctctgct aaccatgttc atgccttctt ctttttccta
     2101 cagctcctgg gcaacgtgct ggttattgtg ctgtctcatc attttggcaa agaattctgc
     2161 agtcgacggt accgcgggcc cgggatccac cggtcgccac catgggcgcc ccaaagaaga
     2221 agcggaaggt cggtatccac ggagtcccag cagccagcga cctggtgctg ggcctggaca
     2281 tcggcatcgg cagcgtgggc gtgggcatcc tgaacaaggt gaccggcgag atcatccaca
     2341 agaacagtcg catcttccct gctgctcagg ctgagaacaa cctggtgcgc cgcaccaacc
     2401 gccagggtcg ccggcttgct cgccgcaaga agcaccggcg cgtgcgcctg aaccgcctgt
     2461 tcgaggagag cggcctgatc accgacttca ccaagatcag catcaacctg aacccctacc
     2521 agctgcgcgt gaagggcctg accgacgagc tgagcaacga ggagctgttc atcgccctga
     2581 agaacatggt gaagcaccgc ggcatcagct acctggacga cgccagcgac gacggcaaca
     2641 gcagcgtggg cgactacgcc cagatcgtga aggagaacag caagcagctg gagaccaaga
     2701 cccccggcca gatccagctg gagcgctacc agacctacgg ccagctgcgc ggcgacttca
     2761 ccgtggagaa ggacggcaag aagcaccgcc tgatcaacgt gttccccacc agcgcctacc
     2821 gcagcgaggc cctgcgcatc ctgcagaccc agcaggagtt caacccccag atcaccgacg
     2881 agttcatcaa ccgctacctg gagatcctga ccggcaagcg caagtactac cacggccccg
     2941 gcaacgagaa gagccgcacc gactacggcc gctaccgcac cagcggcgag accctggaca
     3001 acatcttcgg catcctgatc ggcaagtgca ccttctaccc cgacgagttc cgcgccgcca
     3061 aggccagcta caccgcccag gagttcaatc tgctgaacga cctgaacaac ctgaccgtgc
     3121 ccaccgagac caagaagctg agcaaggagc agaagaacca gatcatcaac tacgtgaaga
     3181 acgagaaggc tatgggcccc gccaagctgt tcaagtacat cgccaagctg ctgagctgcg
     3241 acgtggccga catcaagggc taccgcatcg acaagagcgg caaggccgag atccacacct
     3301 tcgaggccta ccgcaagatg aagaccctgg agaccctgga catcgagcag atggaccgag
     3361 agaccctgga caagctggcc tacgtgctga ccctgaacac cgagcgcgag ggcatccagg
     3421 aggccctgga gcacgagttc gccgacggca gcttcagcca gaaacaggtg gacgagctgg
     3481 tgcagttccg caaggccaac agcagcatct tcggcaaggg ctggcacaac ttcagcgtga
     3541 agctgatgat ggagctgatc cccgagctgt acgagaccag cgaggagcag atgaccatcc
     3601 tgacccgcct gggcaagcag aagaccacca gcagcagcaa caagaccaag tacatcgacg
     3661 agaagctgct gaccgaggag atctacaacc ccgtggtggc caagagcgtg cgccaggcca
     3721 tcaagatcgt gaacgccgcc atcaaggagt acggcgactt cgacaacatc gtgatcgaga
     3781 tggcccgcga gaccaacgag gacgacgaga agaaggccat ccagaagatc cagaaggcca
     3841 acaaggacga gaaggacgcc gccatgctga aggccgccaa ccagtacaac ggcaaggccg
     3901 agctgcccca cagcgtgttc cacggccaca agcagctggc caccaagatc cgcctgtggc
     3961 accagcaggg cgagcgctgc ctgtacaccg gcaagaccat cagcatccac gacctgatca
     4021 acaacagcaa ccagttcgag gtggaccaca tcctgcccct gagcatcacc ttcgacgaca
     4081 gcctggccaa caaggtgctg gtgtacgcca ccgccaacca ggagaagggc cagcgcaccc
     4141 cctaccaggc cctggacagc atggacgacg cctggagctt ccgcgagctg aaggccttcg
     4201 tgcgcgagag caagaccctg agcaacaaga agaaggagta tctgctgacc gaggaggaca
     4261 tcagcaagtt cgacgtgcgc aagaagttca tcgagcgcaa cctggtggac acccgctacg
     4321 ccagccgcgt ggtgctgaac gccctgcagg agcacttccg cgcccacaag atcgacacca
     4381 aggtgagcgt ggtgcgcggc cagttcacca gccagctgcg ccgccactgg ggcatcgaga
     4441 agacccgcga cacctaccac caccacgccg tggacgccct gatcattgcg gcttctagcc
     4501 agctgaacct gtggaagaag cagaagaaca ccctggtgag ctacagcgag gaccagctgc
     4561 tggacatcga gaccggcgag ctgatcagcg acgacgagta caaggagagc gtgttcaagg
     4621 ccccctacca gcacttcgtg gacaccctga agagcaagga gttcgaggac agcatcctgt
     4681 tcagctacca ggtggacagc aagttcaacc gcaagatcag cgacgccacc atctacgcca
     4741 cccgccaggc caaggtgggc aaggacaagg ccgacgagac ctacgtgctg ggcaagatca
     4801 aggacatcta cacccaggac ggctacgacg ccttcatgaa gatctacaag aaggacaaga
     4861 gcaagttcct gatgtaccgc cacgaccccc agaccttcga gaaggtgatc gagcccatcc
     4921 tggagaacta ccccaacaag cagatcaacg agaaaggcaa ggaggtgccc tgcaacccct
     4981 tcctgaagta caaggaggag cacggctaca tccgcaagta cagcaagaag ggcaacggcc
     5041 ccgagatcaa gagcctgaag tactacgaca gcaagctggg caaccacatc gacatcaccc
     5101 ccaaggacag caacaacaag gtggtgctgc agagcgtgag cccctggcgc gccgacgtgt
     5161 acttcaacaa gaccaccggc aagtacgaga tcctggggct gaagtacgcc gatctgcagt
     5221 ttgagaaagg cacaggcacc tacaagatca gccaggagaa gtacaacgac atcaagaaga
     5281 aggagggcgt ggacagcgac agcgagttca agttcaccct gtacaagaac gaccttctgc
     5341 tggtgaagga caccgagacc aaggagcaac agctgttccg cttcctgagc cgcaccatgc
     5401 ccaagcagaa gcactacgtg gagctgaagc cctacgacaa gcagaagttc gagggcggcg
     5461 aggccctgat caaggtgctg ggcaacgtgg ccaacagcgg ccagtgcaag aagggcctgg
     5521 gcaagagcaa catcagcatc tacaaggtgc gcaccgacgt gctgggcaac cagcacatca
     5581 tcaagaacga gggcgacaag cccaagttgg acttcagcag ggctgacccc aagaagaaga
     5641 ggaaggtgtg agcggccgca ctcctcaggt gcaggctgcc tatcagaagg tggtggctgg
     5701 tgtggccaat gccctggctc acaaatacca ctgagatctt tttccctctg ccaaaaatta
     5761 tggggacatc atgaagcccc ttgagcatct gacttctggc taataaagga aatttatttt
     5821 cattgcaata gtgtgttgga attttttgtg tctctcactc ggaaggacat atgggagggc
     5881 aaatcattta aaacatcaga atgagtattt ggtttagagt ttggcaacat atgcccatat
     5941 gctggctgcc atgaacaaag gttggctata aagaggtcat cagtatatga aacagccccc
     6001 tgctgtccat tccttattcc atagaaaagc cttgacttga ggttagattt tttttatatt
     6061 ttgttttgtg ttattttttt ctttaacatc cctaaaattt tccttacatg ttttactagc
     6121 cagatttttc ctcctctcct gactactccc agtcatagct gtccctcttc tcttatgaag
     6181 atccctcgac ctgcagccca agcttggcgt aatcatggtc atagctgttt cctgtgtgaa
     6241 attgttatcc gctcacaatt ccacacaaca tacgagccgg aagcataaag tgtaaagcct
     6301 ggggtgccta atgagtgagc taactcacat taattgcgtt gcgctcactg cccgctttcc
     6361 agtcgggaaa cctgtcgtgc cagcggatcc gcatctcaat tagtcagcaa ccatagtccc
     6421 gcccctaact ccgcccatcc cgcccctaac tccgcccagt tccgcccatt ctccgcccca
     6481 tggctgacta atttttttta tttatgcaga ggccgaggcc gcctcggcct ctgagctatt
     6541 ccagaagtag tgaggaggct tttttggagg cctaggcttt tgcaaaaagc taacttgttt
     6601 attgcagctt ataatggtta caaataaagc aatagcatca caaatttcac aaataaagca
     6661 tttttttcac tgcattctag ttgtggtttg tccaaactca tcaatgtatc ttatcatgtc
     6721 tggatccgct gcattaatga atcggccaac gcgcggggag aggcggtttg cgtattgggc
     6781 gctcttccgc ttcctcgctc actgactcgc tgcgctcggt cgttcggctg cggcgagcgg
     6841 tatcagctca ctcaaaggcg gtaatacggt tatccacaga atcaggggat aacgcaggaa
     6901 agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg
     6961 cgtttttcca taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga
     7021 ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg
     7081 tgcgctctcc tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg
     7141 gaagcgtggc gctttctcaa tgctcacgct gtaggtatct cagttcggtg taggtcgttc
     7201 gctccaagct gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg
     7261 gtaactatcg tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca
     7321 ctggtaacag gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt
     7381 ggcctaacta cggctacact agaaggacag tatttggtat ctgcgctctg ctgaagccag
     7441 ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg
     7501 gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc
     7561 ctttgatctt ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt
     7621 tggtcatgag attatcaaaa aggatcttca cctagatcct tttaaattaa aaatgaagtt
     7681 ttaaatcaat ctaaagtata tatgagtaaa cttggtctga cagttaccaa tgcttaatca
     7741 gtgaggcacc tatctcagcg atctgtctat ttcgttcatc catagttgcc tgactccccg
     7801 tcgtgtagat aactacgata cgggagggct taccatctgg ccccagtgct gcaatgatac
     7861 cgcgagaccc acgctcaccg gctccagatt tatcagcaat aaaccagcca gccggaaggg
     7921 ccgagcgcag aagtggtcct gcaactttat ccgcctccat ccagtctatt aattgttgcc
     7981 gggaagctag agtaagtagt tcgccagtta atagtttgcg caacgttgtt gccattgcta
     8041 caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc ggttcccaac
     8101 gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc
     8161 ctccgatcgt tgtcagaagt aagttggccg cagtgttatc actcatggtt atggcagcac
     8221 tgcataattc tcttactgtc atgccatccg taagatgctt ttctgtgact ggtgagtact
     8281 caaccaagtc attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa
     8341 tacgggataa taccgcgcca catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt
     8401 cttcggggcg aaaactctca aggatcttac cgctgttgag atccagttcg atgtaaccca
     8461 ctcgtgcacc caactgatct tcagcatctt ttactttcac cagcgtttct gggtgagcaa
     8521 aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa tgttgaatac
     8581 tcatactctt cctttttcaa tattattgaa gcatttatca gggttattgt ctcatgagcg
     8641 gatacatatt tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc acatttcccc
     8701 gaaaagtgcc acctg
//
