LOCUS       sequence-208005-       10784 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  CRISPR/Cas9 vector for mutliplexed genome editing in Ustilago 
            maydis. Expresses U. maydis codon-optimized Cas9 under the hsp70 
            promoter, contains a short version of U6 promoter, is 
            self-replicating.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10784)
  AUTHORS   Schuster M, Schweizer G, Kahmann R
  TITLE     Comparative analyses of secreted proteins in plant pathogenic smut 
            fungi and related basidiomycetes.
  JOURNAL   Fungal Genet Biol. 2018 Mar;112:21-30. doi: 
            10.1016/j.fgb.2016.12.003. Epub 2017 Jan 6.
  PUBMED    28089076
REFERENCE   2  (bases 1 to 10784)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Fungal 
            Genet Biol."; date: "2018-03"; volume: "112:21-30. doi"; pages: " 
            10.1016/j.fgb.2016.12.003. Epub 2017 Jan 6"
COMMENT     Sequence Label: pMS73
FEATURES             Location/Qualifiers
     source          1..10784
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             12..32
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             36..4136
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="DKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     CDS             36..4136
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="DKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     CDS             4146..4172
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             4182..4229
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
     terminator      4250..4502
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     primer_bind     complement(4532..4549)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(4532..4548)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4541..4563)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(4757..4776)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     4876..4898
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(4936..4954)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     CDS             join(7367..7435,7436..8227)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7585..7604)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      8398..8986
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8887..8906
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     9140..9157
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    9274..9295
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(9310..9315,9316..9333,9334..9340)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9348..9364
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     9353..9375
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     9372..9388
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     9372..9388
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 gatccatgcc gcctaagaag aaacgcaagg ttgaggataa gaagtacagc atcggactcg
       61 acatcggtac taactcggta ggatgggcag tcatcacgga tgaatacaag gttccttcca
      121 agaagtttaa ggtccttggt aacaccgacc gccattctat caagaagaac ctcattggcg
      181 ctttgctctt tgactcagga gaaaccgctg aggcgacacg cctcaaacgc acggcacgtc
      241 gacgttatac acgcagaaag aatcgtatct gctatctgca ggaaatcttt tcgaacgaaa
      301 tggcaaaagt tgatgacagc ttcttccatc gcctggagga atcgtttctc gtggaggagg
      361 acaagaagca cgagagacat cctatcttcg gcaacattgt cgatgaggtc gcttaccacg
      421 agaagtaccc tactatctac caccttagaa agaagctcgt agactcaact gacaaagcgg
      481 atcttcgtct gatctatttg gctcttgccc acatgatcaa gttccgtggt cattttctca
      541 tcgaaggcga ccttaatccc gacaactcgg acgtcgataa gctgtttatc cagctcgtac
      601 agacatacaa ccagctcttc gaagaaaacc caattaatgc ttccggtgtt gatgcaaaag
      661 ctatcctcag cgcgagattg agcaagagca gacgcctcga aaacctcatc gcccaattgc
      721 ctggtgaaaa gaagaacggc ttgttcggca atcttattgc cctgagcctt ggcctcactc
      781 cgaacttcaa gtcgaacttc gatcttgcgg aagatgccaa gctgcaactc tccaaggaca
      841 cgtacgatga tgacttggac aatctccttg cccagattgg cgatcaatac gccgatcttt
      901 tcctcgcggc gaagaacttg tcggacgcaa tcttgctctc agacatcctt cgcgtcaaca
      961 ctgagatcac caaagcccct ctctctgcct cgatgatcaa gcgctatgac gaacaccacc
     1021 aggatctcac gctccttaag gcattggtgc gtcagcagtt gcctgagaag tacaaagaga
     1081 ttttctttga tcagtcgaag aacggatacg ctggctacat cgacggtggc gcttctcagg
     1141 aggagttcta caagtttatc aaacccattc ttgagaagat ggatggcacg gaggagctcc
     1201 tcgtcaagct gaatcgcgag gacctcctcc gtaagcaacg tacgttcgac aatggctcga
     1261 ttccacacca gattcatctg ggcgaactcc acgccatcct caggaggcag gaggacttct
     1321 atcccttcct caaggataat cgagagaaaa ttgagaagat cctcacattc cgcatcccct
     1381 attatgtagg cccactcgct cgcggaaact ctcgctttgc ctggatgacc cgcaagtcgg
     1441 aagaaacaat caccccgtgg aacttcgaag aggtggtgga caagggtgca tctgcgcagt
     1501 cgtttattga gaggatgaca aactttgata agaacctccc gaatgagaaa gtcctgccaa
     1561 aacattccct cctgtatgaa tacttcacgg tctataacga actgacaaag gtgaagtacg
     1621 tgaccgaggg tatgcgtaag cctgcctttc tttcgggtga gcagaagaaa gctattgtcg
     1681 acttgttgtt caagaccaac cgcaaggtca ctgtcaagca actgaaggaa gattacttca
     1741 agaaaatcga gtgttttgat tcggtagaga tctcgggcgt cgaggacagg ttcaacgcct
     1801 ctctcggcac ctatcacgat cttctcaaga tcatcaagga caaagacttt cttgacaacg
     1861 aagagaacga ggatattctc gaggacatcg tgctcaccct cactttgttc gaagatcgcg
     1921 aaatgattga ggaacgtctt aagacatatg ctcacttgtt cgacgacaaa gtgatgaagc
     1981 agctgaagcg taggcgatac acaggttggg gccgcctctc gcgcaagctg attaacggta
     2041 tccgcgacaa gcaatccggc aagacaatct tggatttcct taagagcgac ggttttgcta
     2101 accgcaactt catgcagctc atccacgacg acagccttac gttcaaggag gacatccaga
     2161 aggcccaggt ttccggacaa ggtgactctc tccatgagca catcgctaac ctggcgggaa
     2221 gccccgcgat caagaaaggt atcctccaga ccgtcaaagt tgtggacgag ctggtcaagg
     2281 taatgggccg acacaaaccg gagaacattg ttatcgagat ggcacgagag aatcagacga
     2341 cccagaaagg ccaaaagaac tccagagaac gtatgaaacg aatcgaagag ggtatcaagg
     2401 aactgggatc gcagatcctg aaggagcacc ccgttgagaa cacgcagctc caaaacgaaa
     2461 agctgtacct ctactacttg caaaatggta gggatatgta cgtcgaccag gaactggata
     2521 ttaatcgtct gtccgactac gacgttgacc acatcgtgcc ccaatcgttt ctcaaggatg
     2581 actcgatcga taataaagta cttacgcgct cagacaagaa ccgaggtaaa tcggacaatg
     2641 tcccatcgga ggaagtcgtg aagaagatga agaactattg gcgccaactt cttaacgcaa
     2701 agctgatcac ccagaggaaa ttcgacaacc tcaccaaagc agaacgcggc ggcctctccg
     2761 agctcgacaa ggctggattt atcaagcgtc agctcgtcga aacgcgtcag attaccaagc
     2821 acgtcgcaca gatcctggat agccgcatga acacaaagta cgacgaaaac gacaagctca
     2881 tccgtgaggt taaggtcatc accttgaagt cgaaactcgt gtcggacttc cgcaaagatt
     2941 ttcagttcta taaagttaga gagatcaaca actaccacca tgcgcatgac gcctacctca
     3001 atgccgtcgt gggcaccgca cttattaaga aatacccgaa gctcgagtcc gagtttgtct
     3061 acggcgatta caaggtatac gacgttcgca agatgattgc caaatcggag caggagatcg
     3121 gtaaggccac tgccaagtac ttcttttact cgaacatcat gaatttcttc aaaacagaaa
     3181 tcaccctcgc caacggcgag attcgcaaac gaccactcat cgagactaac ggtgaaacgg
     3241 gagagatcgt ctgggataag ggccgagact ttgctacggt tcgaaaggtc ctttcgatgc
     3301 ctcaagtgaa catcgtcaag aaaacggagg tccaaaccgg tggcttcagc aaggagtcga
     3361 ttctgccgaa acgcaattcg gacaaattga ttgcacgcaa gaaggattgg gaccctaaga
     3421 aatatggcgg cttcgattca ccgacagtgg cctattcggt tctggtcgtc gcgaaagtgg
     3481 agaagggcaa gtcaaagaag ctcaagtcag tgaaggagct cctgggaatc accatcatgg
     3541 aacgttcctc ttttgagaag aaccctatcg actttctcga ggctaagggc tacaaagagg
     3601 tcaagaaaga tctcatcatc aaactcccaa aatactcact tttcgagctc gagaacggcc
     3661 gtaaacgaat gctggcgagc gcaggagagc ttcaaaaggg aaatgaactg gctttgccct
     3721 ccaagtacgt caacttcctc tacctcgcaa gccattatga gaagttgaag ggtagccccg
     3781 aggacaacga acaaaagcag ctcttcgtgg agcaacacaa acattacctg gatgaaatca
     3841 tcgagcaaat ctcggagttt agcaagcgag tgatcttggc tgatgccaac ctcgacaagg
     3901 tgttgtctgc ctacaacaag catcgagata agccgattcg cgagcaggcc gagaacatca
     3961 tccacctctt cactctcact aacttgggtg cgcctgcggc gtttaaatac tttgacacga
     4021 ccatcgaccg caagcgttac acaagcacga aggaagtcct cgacgctaca ctgatccatc
     4081 agtcgatcac cggtctgtac gaaacccgca tcgatctgtc tcaactgggc ggtgacagcg
     4141 gcggctaccc atacgatgtg cccgattacg ctagcggcgg aaagcgtccc gcagccacta
     4201 agaaggctgg acaggccaag aagaagaagt gagcggccgc ccggctgcag atcgttcaaa
     4261 catttggcaa taaagtttct taagattgaa tcctgttgcc ggtcttgcga tgattatcat
     4321 ataatttctg ttgaattacg ttaagcatgt aataattaac atgtaatgca tgacgttatt
     4381 tatgagatgg gtttttatga ttagagtccc gcaattatac atttaatacg cgatagaaaa
     4441 caaaatatag cgcgcaaact aggataaatt atcgcgcgcg gtgtcatcta tgttactaga
     4501 tccgatgata agctgtcaaa catgagaatt cactggccgt cgttttacaa cgtcgtgact
     4561 gggaaaaccc tggcgttacc caacttaatc gccttgcagc acatccccct ttcgccagct
     4621 ggcgtaatag cgaagaggcc cgcaccgatc gcccttccca acagttgcgc agcctgaatg
     4681 gcgaatggcg cctgatgcgg tattttctcc ttacgcatct gtgcggtatt tcacaccgca
     4741 tatggtgcac tctcagtaca atctgctctg atgccgcata gttaagccag ccccgacacc
     4801 cgccaacacc cgctgacgcg ccctgacggg cttgtctgct cccggcatcc gcttacagac
     4861 aagctgtgac cgtctccggg agctgcatgt gtcagaggtt ttcaccgtca tcaccgaaac
     4921 gcgcgagacg aaagggcctc gtgatacgcc tatttttata ggttaatgtc atgataataa
     4981 tggtttctta ggcggccgca cgctaagtgg agttgtccga gtccccgaat cacaagaatt
     5041 aggctcgtgc tctgtgagat ctctcgcgaa acccagatga aggaaaaaaa tcggaagatc
     5101 gcggaagaag tggggttcgc atggtctaac attgtcgcat tcctcacagt ttcggctgga
     5161 aacggcaggg acaatcacga gaaatgcgcg acgagtattc agttgtccaa aattatgtca
     5221 gcttgaaagt tggaaacggc ggaagaaaat tcgtcaggag ggcgttgcgg ctagggtgaa
     5281 gaacggagac aatttcggct ttgaaattgg ctcgactctg attggatcat gacgcactgc
     5341 tggtccacaa ccgtgtcgaa ggtgccgtct ttactacagg tccgctggaa gcaaaatgga
     5401 aaaagccgct ggagcccgac tacagagccg ccgtgttttg gtaatcagtc ggcaaatagg
     5461 tcagcacagc gcagcgtgac aggttcttgc aatttacagc acagctcgtc cgtctacgac
     5521 tttgcacacc acaaagtgtg cggggagcaa aggagccgat cttggtcgcg cgcaaagcca
     5581 aggagtcttg aacctgagag tgtgcgtgtc ttgtgacgct tgcccttctg tactttgctg
     5641 tgacactacc accacatctg tcttggcttt ttgttcatac atccacaccg accatgtcgc
     5701 tattcaacgt cagcaacggt cttcgtaccg ctctccgacc ttctgttgcc agctcttcgc
     5761 gcgttgctgc cttttccaca accgccgctg cccgtctcgc cacacccacc tctgacaacg
     5821 ttggcagttc gggcaagcct cagcacttga agcagttcaa gatctaccga tggaaccctg
     5881 acaagccctc ggagaagcct cgtctgcagt cgtacacact ggacctcaac cagaccggtc
     5941 caatggttct cgacgcgctc atcaaaatca agaacgaaat tgaccctacg ctcaccttcc
     6001 gtcgctcgtg ccgtgagggt atctgcggtt cgtgcgctat gaatattgac ggtgtcaaca
     6061 ccctcgcctg cctctgccgg atcgacaagc agaatgacac caagatctac cccttgccgc
     6121 acatgtacat tgtcaaggac ctcgtgccag acttgaccca gttctacaag cagtaccgat
     6181 ccatcgagcc tttcctcaag tccaacaaca ccccttctga gggtgaacat cttcagtcgc
     6241 ccgaggagcg tcgtcgactc gacggtctgt acgagtgcat tctgtgcgcg tgctgctcca
     6301 catcctgccc ctcttactgg tggaatcagg acgagtacct tggccccgcc gtgctcatgc
     6361 aggcgtaccg atggatggcc gactcgcgtg acgactttgg tgaggagcga agacagaagc
     6421 tcgagaacac cttttcgctc taccgatgcc ttaccatcat gaactgctcc aggacctgcc
     6481 ccaagaacct caaccctggt aaggcaattg cacagatcaa gaaggacatg gccgtcggcg
     6541 cacccaaggc ttccgagcgc cctatcatgg cttcgtcgta atcttgatat atcatatcgt
     6601 tctttcctca gcacttcttt tgtcaatttc aaaagtatct aattgcattc aactccgctt
     6661 gtggtttgtt gttcagtgag agtggaaacg ctacgggcaa gatgagggca gtgttctggc
     6721 gacggaaaag tgtgcaagtg tctggcctgc gtcctcgctg gttccagcag ccgatgcagg
     6781 acgtgtacct agcgatttct tcgacagcct attgtggcag ccgcgattcg ccacaatcgt
     6841 acgtgcggcc gccaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat
     6901 ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc
     6961 aataatatta ccgatcctcg atctttgtgc aagctagccc gcctcggcag caacaaagca
     7021 gccgagcaag aagcagtact tgccttctga atcgtgaatg ggttacgttc ttcaccgctg
     7081 tgatcagcga atcatgaatc aaatcatgag ggcattgctg atcatgaatc aaatcatgag
     7141 ggcatttaaa aattcagtct gagtcgtgag tagcaagtcg gttctggatc ggatggcatt
     7201 catgaatcac agggtcgtga atcatgaatg ttcaagtccc cttttctcga gaggctggtg
     7261 ggatcggtgc gaatcacgaa tcatgattgt aattcattga gtgaaggagt ttcgcagcca
     7321 cccacagtac tagaatcacg aatgacaata ttgaaaaagg aagagtatga gtattcaaca
     7381 tttccgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt ttgctcaccc
     7441 agaaacgctg gtgaaagtaa aagatgctga agatcagttg ggtgcacgag tgggttacat
     7501 cgaactggat ctcaacagcg gtaagatcct tgagagtttt cgccccgaag aacgttttcc
     7561 aatgatgagc acttttaaag ttctgctatg tggcgcggta ttatcccgta ttgacgccgg
     7621 gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat gacttggttg agtactcacc
     7681 agtcacagaa aagcatctta cggatggcat gacagtaaga gaattatgca gtgctgccat
     7741 aaccatgagt gataacactg cggccaactt acttctgaca acgatcggag gaccgaagga
     7801 gctaaccgct tttttgcaca acatggggga tcatgtaact cgccttgatc gttgggaacc
     7861 ggagctgaat gaagccatac caaacgacga gcgtgacacc acgatgcctg tagcaatggc
     7921 aacaacgttg cgcaaactat taactggcga actacttact ctagcttccc ggcaacaatt
     7981 aatagactgg atggaggcgg ataaagttgc aggaccactt ctgcgctcgg cccttccggc
     8041 tggctggttt attgctgata aatctggagc cggtgagcgt gggtctcgcg gtatcattgc
     8101 agcactgggg ccagatggta agccctcccg tatcgtagtt atctacacga cggggagtca
     8161 ggcaactatg gatgaacgaa atagacagat cgctgagata ggtgcctcac tgattaagca
     8221 ttggtaactg tcagaccaag tttactcata tatactttag attgatttaa aacttcattt
     8281 ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta
     8341 acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg
     8401 agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc
     8461 ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag
     8521 cagagcgcag ataccaaata ctgtccttct agtgtagccg tagttaggcc accacttcaa
     8581 gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc
     8641 cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc
     8701 gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta
     8761 caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag
     8821 aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct
     8881 tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga
     8941 gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc
     9001 ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt
     9061 atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata ccgctcgccg
     9121 cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaagagc gcccaatacg
     9181 caaaccgcct ctccccgcgc gttggccgat tcattaatgc agctggcacg acaggtttcc
     9241 cgactggaaa gcgggcagtg agcgcaacgc aattaatgtg agttagctca ctcattaggc
     9301 accccaggct ttacacttta tgcttccggc tcgtatgttg tgtggaattg tgagcggata
     9361 acaatttcac acaggaaaca gctatgacca tgattacgcc aagcttcgac cagagagaga
     9421 ggcagagaga gagagagaga tggctcttca gcgccgcttc gcgcgtcctt cacgattatt
     9481 cgtgattact gtccaccggt ccctcttact cagaactgcc ggaacgaatt cgtgatttac
     9541 accaaacacg cgctgtcaca gtcacgagtc catgagccgt gagccccgct tcagatcctg
     9601 ttttctctta ttcagcgtaa caacacaaaa cagaatttct tctaaacacc cttgcaattc
     9661 gcgcacaccc ctgtagcagt ctgtcagcat tcaaaattcc attctacaac ggtacctgcc
     9721 aagagtcgaa cgccgagaag gcgaaatgca ccggacactg gggatgctgc tggaagatgg
     9781 gccgacaaag gactcagctg ctactgagtc ataaatagtt cggattgacg gctgagtcga
     9841 aagcgttcgt atatattggt ctgattcacg attcggaaga aattctacaa ggttgcgcag
     9901 ctcaggtcat cttaagttta ctctcaccgt ctcgagtggc ggcagatgtg agtcgtgtgc
     9961 tacaaaacgt gaatcgacac gcgcagggcg gaaccaaaaa aaacccccac cccgctgtca
    10021 agttgacaaa tcaacacatt tgttccaagt tcaaatcggg aaatcaaaaa ttagggccag
    10081 atcagcgatc aggaatggta atcgggtaac agaggtcgca aaatcgtcta gaaaatggaa
    10141 gaagaacgtg gtaactacca gcgagttctg caaacttcaa aaaaaaaatc tgggcacgat
    10201 gaaagttgag ctaacgctga cgctcacaaa tggcgtggct aaaggaagcg agacaatcgg
    10261 aaaattgttc tctcgggcac cacaaagctg ttgttagtcg ctgaagaaca attccaactg
    10321 attccgccgc cttcctattg cgtcagcctt gtacctaagc tgccgagtaa cgtcactcaa
    10381 cctctctttt cagactgctt tgctccgcga atacttttct tctatgcgct caagaaaatg
    10441 acacagcaca ccaagctctg caaactttct tcgctaatct gacgcgaaat gtgagccatt
    10501 tcttctcgcc tgcaatggca atgcgtctgt gcggcgatga gaatcacgat gcggaatggg
    10561 tggctggaag ttcatagaga tgctgagttg ttggagcgac atggtacata agcatgagtc
    10621 tgtcctgatt tccaccctcc cgtctttcat caactttctc gtctgaccct tccgttgcac
    10681 ataccccttt cgcaaccaag ttacgacatc agcacacagc gatcctgcta ttccacacag
    10741 gacttccttc cccttcacca cagcaaaagt tttagatttc acag
//
