LOCUS       sequence-20801-e         616 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 616)
  AUTHORS   Alberti S, Gitler AD, Lindquist S
  TITLE     A suite of Gateway cloning vectors for high-throughput genetic 
            analysis in Saccharomyces cerevisiae.
  JOURNAL   Yeast. 2007 Oct . 24(10):913-9.
  PUBMED    17583893
REFERENCE   2  (bases 1 to 616)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Yeast. 2007
            Oct . 24(10):913-9."
COMMENT     Sequence Label: pAG306GPD-ECFP-ccdB
FEATURES             Location/Qualifiers
     source          1..616
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     224..245
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(303..319)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(303..319)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     promoter        complement(325..588)
                     /gene="S. cerevisiae TDH3"
                     /label=GAP promoter
                     /note="promoter for glyceraldehyde-3-phosphate 
                     dehydrogenase; also known as the TDH3 promoter"
     primer_bind     complement(408..431)
                     /label=GPDpro-F
                     /note="S. cerevisiae GPD promoter, forward primer"
ORIGIN
        1 cgctcctggg agtagccttc gggcatggcg gacttgaaga agtcgtgctg cttcatgtgg
       61 tcggggtagc ggctgaagca ctgcacgccc caggtcaggg tggtcacgag ggtgggccag
      121 ggcacgggca gcttgccggt ggtgcagatg aacttcaggg tcagcttgcc gtaggtggca
      181 tcgccctcgc cctcgccgga cacgctgaac ttgtggccgt ttacgtcgcc gtccagctcg
      241 accaggatgg gcaccacccc ggtgaacagc tcctcgccct tgctcaccat tttgtcccgg
      301 gggatccact agttctagaa tccgtcgaaa ctaagttctg gtgttttaaa actaaaaaaa
      361 agactaacta taaaagtaga atttaagaag tttaagaaat agatttacag aattacaatc
      421 aatacctacc gtctttatat acttattagt caagtagggg aataatttca gggaactggt
      481 ttcaaccttt tttttcagct ttttccaaat cagagagagc agaaggtaat agaaggtgta
      541 agaaaatgag atagatacat gcgtgggtca attgccttgt gtcatcattt actccaggca
      601 ggttgcatca ctccat
//
