LOCUS       sequence-208018-        1071 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Retroviral expression of Flag-SFXN1 in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1071)
  AUTHORS   Kory N, Wyant GA, Prakash G, Uit de Bos J, Bottanelli F, Pacold ME, 
            Chan SH, Lewis CA, Wang T, Keys HR, Guo YE, Sabatini DM
  TITLE     SFXN1 is a mitochondrial serine transporter required for one-carbon 
            metabolism.
  JOURNAL   Science. 2018 Nov 16;362(6416). pii: 362/6416/eaat9528. doi: 
            10.1126/science.aat9528.
  PUBMED    30442778
REFERENCE   2  (bases 1 to 1071)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Science."; 
            date: "2018-11-16"; volume: "362(6416). pii: 362/6416/eaat9528. 
            doi"; pages: " 10.1126/science.aat9528"
COMMENT     Sequence Label: pMXs_FLAG-SFXN1
FEATURES             Location/Qualifiers
     source          1..1071
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             4..69
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
ORIGIN
        1 atggactaca aggaccacga cggtgactac aaggaccacg acatcgacta caaggacgac
       61 gacgacaagg ggcatggcac cggcagcacc ggcagcgaat tcatgagcgg tgaactgcct
      121 ccaaacataa atattaagga accacgatgg gatcaatcta cgttcatcgg aagggctaac
      181 catttcttta cagtgacaga cccgaggaat attttgttga ccaacgagca attggagtct
      241 gcaaggaaaa tcgtccatga ttaccgacaa ggtattgtgc cgccaggact tactgaaaac
      301 gaactttgga gagcgaaata catttatgac tctgcattcc atccagacac tggagaaaag
      361 atgatcctga ttggtcgcat gtcagctcaa gtccccatga atatgactat aactggctgt
      421 atgatgacat tttacagaac aacacccgct gttctctttt ggcaatggat aaatcagagc
      481 ttcaacgccg tagtaaacta cacaaacaga tccggcgacg ctccactgac tgtcaacgaa
      541 ctcggaactg cctacgtgtc cgccacgact ggggccgtcg ccaccgccct gggacttaat
      601 gcactgacga agcatgttag ccctctcata ggccggttcg ttccgttcgc ggccgtagca
      661 gccgctaact gtatcaacat acctctgatg agacagaggg aactgaaggt cggaatccca
      721 gtgaccgatg agaatggcaa tcgactgggc gagagtgcaa acgcagctaa acaggcgata
      781 acgcaagtgg tagttagtag gatattgatg gcggctcccg gaatggcgat accccccttc
      841 attatgaaca ccctggagaa aaaggcgttt ttgaaacgct tcccatggat gtctgcgcca
      901 atacaggtcg ggctcgtagg tttctgtttg gtctttgcta ctcctctgtg ttgcgcactc
      961 ttcccccaaa aaagcagtat gtcagttaca agcctcgagg ctgagctcca agctaaaatt
     1021 caggagtctc accctgaatt gcgacgagtt tactttaata agggacttta a
//
