LOCUS       sequence-208022-        1080 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Retroviral expression of Flag-SFXN3 in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1080)
  AUTHORS   Kory N, Wyant GA, Prakash G, Uit de Bos J, Bottanelli F, Pacold ME, 
            Chan SH, Lewis CA, Wang T, Keys HR, Guo YE, Sabatini DM
  TITLE     SFXN1 is a mitochondrial serine transporter required for one-carbon 
            metabolism.
  JOURNAL   Science. 2018 Nov 16;362(6416). pii: 362/6416/eaat9528. doi: 
            10.1126/science.aat9528.
  PUBMED    30442778
REFERENCE   2  (bases 1 to 1080)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Science."; 
            date: "2018-11-16"; volume: "362(6416). pii: 362/6416/eaat9528. 
            doi"; pages: " 10.1126/science.aat9528"
COMMENT     Sequence Label: pMXs_FLAG-SFXN3
FEATURES             Location/Qualifiers
     source          1..1080
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             4..69
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
ORIGIN
        1 atggactaca aggaccacga cggtgactac aaggaccacg acatcgacta caaggacgac
       61 gacgacaagg ggcatggcac cggcagcacc ggcagcgaat tcatggaaag caaaatgggt
      121 gaattgcctt tagacatcaa catccaggaa cctcgctggg accaaagtac tttcctgggc
      181 agagcccggc actttttcac tgttactgat cctcgaaatc tgctgctgtc cggggcacag
      241 ctggaagctt ctcggaacat cgtgcagaac tacagggccg gcgtggtgac cccagggatc
      301 accgaggacc agctgtggag ggccaagtat gtgtatgact ccgccttcca tccggacaca
      361 ggggagaagg tggtcctgat tggccgcatg tcagcccagg tgcccatgaa catgaccatc
      421 actggctgca tgctcacatt ctacaggaag accccaaccg tggtgttctg gcagtgggtg
      481 aatcagtcct tcaatgccat tgttaactac tccaaccgca gtggtgacac tcccatcact
      541 gtgaggcagc tggggacagc ctatgtgagt gccaccactg gagctgtggc cacggccctg
      601 ggactcaaat ccctcaccaa gcacctgccc cccttggtcg gcagatttgt gccctttgca
      661 gcagtggcag ctgccaactg catcaacatc cccctgatga ggcagagaga gctgcaggtg
      721 ggcatcccgg tggctgatga ggcaggtcag aggcttggct actcggtgac tgcagccaag
      781 cagggaatct tccaggtggt gatttcaaga atctgcatgg cgattcctgc catggccatc
      841 ccaccactga tcatggacac tctggagaag aaagacttcc tgaagcgccg cccctggctg
      901 ggggcacccc tgcaggtggg actggtgggc ttctgcctgg tatttgcaac ccccctgtgc
      961 tgtgccctat tcccccagaa gagctccata cacataagca acctggaacc agagctgaga
     1021 gctcagatcc atgagcaaaa ccccagcgtt gaagtggtct actacaacaa ggggctttga
//
