LOCUS       sequence-208023-        1116 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Retroviral expression of Flag-SFXN4 in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1116)
  AUTHORS   Kory N, Wyant GA, Prakash G, Uit de Bos J, Bottanelli F, Pacold ME, 
            Chan SH, Lewis CA, Wang T, Keys HR, Guo YE, Sabatini DM
  TITLE     SFXN1 is a mitochondrial serine transporter required for one-carbon 
            metabolism.
  JOURNAL   Science. 2018 Nov 16;362(6416). pii: 362/6416/eaat9528. doi: 
            10.1126/science.aat9528.
  PUBMED    30442778
REFERENCE   2  (bases 1 to 1116)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Science."; 
            date: "2018-11-16"; volume: "362(6416). pii: 362/6416/eaat9528. 
            doi"; pages: " 10.1126/science.aat9528"
COMMENT     Sequence Label: pMXs_FLAG-SFXN4
FEATURES             Location/Qualifiers
     source          1..1116
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             4..69
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
ORIGIN
        1 atggactaca aggaccacga cggtgactac aaggaccacg acatcgacta caaggacgac
       61 gacgacaagg ggcatggcac cggcagcacc ggcagcgaat tcatgtccct ggaacaggag
      121 gaggaaacgc aacctgggcg gctcctagga cgcagagacg ccgtccccgc cttcattgag
      181 cccaacgtgc gcttctggat caccgagcgc caatccttta ttcgacgatt tcttcaatgg
      241 acagaattat tagatcctac aaatgtgttc atttcagttg aaagtataga aaactcgagg
      301 caactattgt gcacaaatga agatgtttcc agccctgcct cggcggacca aaggatacag
      361 gaagcttgga agcggagtct tgcaacagtg catcccgaca gcagcaacct gatccccaag
      421 ctttttcgac ctgcagcgtt cctgcctttc atggcgccca cggtattttt gtcaatgacg
      481 ccactgaaag ggatcaagtc cgtgatttta cctcaggttt tcctctgtgc ctacatggca
      541 gcgttcaaca gcatcaatgg aaacagaagt tacacttgta agccactaga aagatcatta
      601 ctaatggcgg gagccgttgc ttcttcaact ttcttaggag taatccctca gtttgtccag
      661 atgaagtatg gcctgactgg cccttggatt aaaagactct tacctgtgat cttcctcgtg
      721 caagccagtg gaatgaatgt ctacatgtcc cgaagtcttg aatccattaa ggggattgcg
      781 gtcatggaca aggaaggcaa tgtcctgggt cattccagaa ttgctgggac aaaggctgtt
      841 agagaaacgc tagcatccag aatagtgctg tttgggacct cagctctgat tcctgaagtc
      901 ttcacctact tttttaaaag gacccagtat ttcaggaaaa acccagggtc attgtggatt
      961 ttgaaactgt cttgtactgt cctggcaatg ggactgatgg tgccattttc ttttagtata
     1021 tttccacaga ttggacagat acagtactgt agtcttgaag agaaaattca gtctccaaca
     1081 gaagaaacag aaatctttta tcacagaggg gtgtag
//
