LOCUS       sequence-208026-        1086 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Retroviral expression of flag-tagged yeast FSF1 in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1086)
  AUTHORS   Kory N, Wyant GA, Prakash G, Uit de Bos J, Bottanelli F, Pacold ME, 
            Chan SH, Lewis CA, Wang T, Keys HR, Guo YE, Sabatini DM
  TITLE     SFXN1 is a mitochondrial serine transporter required for one-carbon 
            metabolism.
  JOURNAL   Science. 2018 Nov 16;362(6416). pii: 362/6416/eaat9528. doi: 
            10.1126/science.aat9528.
  PUBMED    30442778
REFERENCE   2  (bases 1 to 1086)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Science."; 
            date: "2018-11-16"; volume: "362(6416). pii: 362/6416/eaat9528. 
            doi"; pages: " 10.1126/science.aat9528"
COMMENT     Sequence Label: pMXs_FLAG-FSF1
FEATURES             Location/Qualifiers
     source          1..1086
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             4..69
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
ORIGIN
        1 atggactaca aggaccacga cggtgactac aaggaccacg acatcgacta caaggacgac
       61 gacgacaagg ggcatggcac cggcagcacc ggcagcgaat tcatggcgtc tagcgttcca
      121 ggcccgattg accttccaga gagcaggtat gacctttcta cttactgggg ccggatacga
      181 cattgtgctg agattagcga tcccacaatg ttgctcacca cagaaaaaga tctcgctcac
      241 gcaagggaga taatttctgc atatcggcac ggggaactga aagaaacaac acccgagttt
      301 tggagagcga agaagcagct tgactccaca gtgcatcccg atacagggaa gacggtactg
      361 ctccccttta gaatgtctag caatgttctg tctaatcttg ttgtgacagt tggtatgctc
      421 actccagggc tgggcacggc gggaaccgtg ttttggcagt gggcaaatca gtcactcaat
      481 gtagccgtta acagtgcaaa cgctaataaa agccatccta tgtctacttc tcaacttctc
      541 actaattacg ctgctgcagt cactgcgtcc tgcggtgtcg cattggggct gaataacttg
      601 gttcctcgcc tgaagaacat ctctccacat tccaaactta tactgggccg cctcgttccc
      661 ttcgctgcag tcgtaagcgc cggtatagta aatgtattcc tgatgagggg caacgagatc
      721 cggaaaggta taagcgtctt tgattctaac ggtgatgaag tgggaaagag taagaaggcg
      781 gccttcatgg cggtcggaga gactgctctt agtcgggtga ttaatgctac accaacaatg
      841 gtgattcctc cactgatact ggtccgcctg cagcgaggtg tgcttaaggg aaagtctctt
      901 ggtgtccaga ctctcgccaa tctgggactg atctccgtga cgatgtttag tgccttgcca
      961 ttcgcccttg gcattttccc gcaacgccaa gcgatccact tgaataaact cgaacccgaa
     1021 ctgcatggca agaaggacaa agatggaaag cctatcgaga aggtttattt caaccgagga
     1081 atttaa
//
