LOCUS       sequence-20803-e         467 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 467)
  AUTHORS   Alberti S, Gitler AD, Lindquist S
  TITLE     A suite of Gateway cloning vectors for high-throughput genetic 
            analysis in Saccharomyces cerevisiae.
  JOURNAL   Yeast. 2007 Oct . 24(10):913-9.
  PUBMED    17583893
REFERENCE   2  (bases 1 to 467)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Yeast. 2007
            Oct . 24(10):913-9."
COMMENT     Sequence Label: pAG413GAL-ECFP-ccdB
FEATURES             Location/Qualifiers
     source          1..467
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     211..232
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(290..306)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(290..306)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     primer_bind     complement(327..350)
                     /label=GAL1
                     /note="S. cerevisiae GAL1 promoter, forward primer"
ORIGIN
        1 agccttcggg catggcggac ttgaagaagt cgtgctgctt catgtggtcg gggtagcggc
       61 tgaagcactg cacgccccag gtcagggtgg tcacgagggt gggccagggc acgggcagct
      121 tgccggtggt gcagatgaac ttcagggtca gcttgccgta ggtggcatcg ccctcgccct
      181 cgccggacac gctgaacttg tggccgttta cgtcgccgtc cagctcgacc aggatgggca
      241 ccaccccggt gaacagctcc tcgcccttgc tcaccatttt gtcccggggg atccactagt
      301 tctagaatcc ggggtttttt ctccttgacg ttaaagtata gaggtatatt aacaattttt
      361 tgttgatact tttattacat ttgaataaga agtaataaaa accgaaaatg ttgaaaatat
      421 tagttaaagt ggttatgcag ttttttcatt tatatatctg ttaatag
//
