LOCUS       sequence-208030-        1710 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  BTK Type 2 promoter for golden gate assembly.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 1710)
  AUTHORS   Leonard SP, Perutka J, Powell JE, Geng P, Richhart DD, Byrom M, Kar 
            S, Davies BW, Ellington AD, Moran NA, Barrick JE
  TITLE     Genetic Engineering of Bee Gut Microbiome Bacteria with a Toolkit 
            for Modular Assembly of Broad-Host-Range Plasmids.
  JOURNAL   ACS Synth Biol. 2018 May 18;7(5):1279-1290. doi: 
            10.1021/acssynbio.7b00399. Epub 2018 Apr 13.
  PUBMED    29608282
REFERENCE   2  (bases 1 to 1710)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acssynbio.7b00399"; journalName: "ACS Synth Biol"; date: 
            "2018-05-18- 18"; volume: "7"; issue: "5"; pages: "1279-1290"
COMMENT     Sequence Label: pBTK102
FEATURES             Location/Qualifiers
     source          1..1710
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     157..176
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     rep_origin      424..1012
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     913..932
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     promoter        1127..1145
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     terminator      complement(1188..1222)
                     /label=lambda t0 terminator
                     /note="minimal transcription terminator from phage lambda 
                     (Scholtissek and Grosse, 1987)"
     CDS             complement(join(1293..1710,1..242))
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
ORIGIN
        1 tcaccgtctt tcattgccat acgaaattcc ggatgagcat tcatcaggcg ggcaagaatg
       61 tgaataaagg ccggataaaa cttgtgctta tttttcttta cggtctttaa aaaggccgta
      121 atatccagct gaacggtctg gttataggta cattgagcaa ctgactgaaa tgcctcaaaa
      181 tgttctttac gatgccattg ggatatatca acggtggtat atccagtgat ttttttctcc
      241 attttagctt ccttagctcc tgaaaatctc gataactcaa aaaatacgcc cggtagtgat
      301 cttatttcat tatggtgaaa gttggaacct cttacgtgcc cgatcaatca tgaccaaaat
      361 cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc
      421 ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct
      481 accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg
      541 cttcagcaga gcgcagatac caaatactgt tcttctagtg tagccgtagt taggccacca
      601 cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc
      661 tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga
      721 taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac
      781 gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga
      841 agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag
      901 ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg
      961 acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag
     1021 caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc
     1081 tgcgttatcc cctgattctg tggataaccg tagtcggtct caaacgtaat acgactcact
     1141 ataggataca gaaacagagg agatattaca tatgtgagac cagaccaata aaaaacgccc
     1201 ggcggcaacc gagcgttctg aacaaatcca gatggagttc tgaggtcatt actggatcta
     1261 tcaacaggag tccaagcgag ctcgatatca aattacgccc cgccctgcca ctcatcgcag
     1321 tactgttgta attcattaag cattctgccg acatggaagc catcacaaac ggcatgatga
     1381 acctgaatcg ccagcggcat cagcaccttg tcgccttgcg tataatattt gcccatggtg
     1441 aaaacggggg cgaagaagtt gtccatattg gccacgttta aatcaaaact ggtgaaactc
     1501 acccagggat tggctgaaac gaaaaacata ttctcaataa accctttagg gaaataggcc
     1561 aggttttcac cgtaacacgc cacatcttgc gaatatatgt gtagaaactg ccggaaatcg
     1621 tcgtggtatt cactccagag cgatgaaaac gtttcagttt gctcatggaa aacggtgtaa
     1681 caagggtgaa cactatccca tatcaccagc
//
