LOCUS       sequence-208032-        1751 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  BTK Type 2 promoter for golden gate assembly.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 1751)
  AUTHORS   Leonard SP, Perutka J, Powell JE, Geng P, Richhart DD, Byrom M, Kar 
            S, Davies BW, Ellington AD, Moran NA, Barrick JE
  TITLE     Genetic Engineering of Bee Gut Microbiome Bacteria with a Toolkit 
            for Modular Assembly of Broad-Host-Range Plasmids.
  JOURNAL   ACS Synth Biol. 2018 May 18;7(5):1279-1290. doi: 
            10.1021/acssynbio.7b00399. Epub 2018 Apr 13.
  PUBMED    29608282
REFERENCE   2  (bases 1 to 1751)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acssynbio.7b00399"; journalName: "ACS Synth Biol"; date: 
            "2018-05-18- 18"; volume: "7"; issue: "5"; pages: "1279-1290"
COMMENT     Sequence Label: pBTK110
FEATURES             Location/Qualifiers
     source          1..1751
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     196..215
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      complement(512..546)
                     /label=lambda t0 terminator
                     /note="minimal transcription terminator from phage lambda 
                     (Scholtissek and Grosse, 1987)"
     CDS             complement(617..1276)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     1191..1210
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     rep_origin      join(1458..1751,1..295)
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg
       61 aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc
      121 cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac
      181 gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct
      241 ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc
      301 cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt
      361 tcctgcgtta tcccctgatt ctgtggataa ccgtagtcgg tctcaaacgc atgtgggagt
      421 ttattcttga cacagatatt tccggatgat ataataactg agtactgtta tacagaaaca
      481 gaggagatat tacatatgtg agaccagacc aataaaaaac gcccggcggc aaccgagcgt
      541 tctgaacaaa tccagatgga gttctgaggt cattactgga tctatcaaca ggagtccaag
      601 cgagctcgat atcaaattac gccccgccct gccactcatc gcagtactgt tgtaattcat
      661 taagcattct gccgacatgg aagccatcac aaacggcatg atgaacctga atcgccagcg
      721 gcatcagcac cttgtcgcct tgcgtataat atttgcccat ggtgaaaacg ggggcgaaga
      781 agttgtccat attggccacg tttaaatcaa aactggtgaa actcacccag ggattggctg
      841 aaacgaaaaa catattctca ataaaccctt tagggaaata ggccaggttt tcaccgtaac
      901 acgccacatc ttgcgaatat atgtgtagaa actgccggaa atcgtcgtgg tattcactcc
      961 agagcgatga aaacgtttca gtttgctcat ggaaaacggt gtaacaaggg tgaacactat
     1021 cccatatcac cagctcaccg tctttcattg ccatacgaaa ttccggatga gcattcatca
     1081 ggcgggcaag aatgtgaata aaggccggat aaaacttgtg cttatttttc tttacggtct
     1141 ttaaaaaggc cgtaatatcc agctgaacgg tctggttata ggtacattga gcaactgact
     1201 gaaatgcctc aaaatgttct ttacgatgcc attgggatat atcaacggtg gtatatccag
     1261 tgattttttt ctccatttta gcttccttag ctcctgaaaa tctcgataac tcaaaaaata
     1321 cgcccggtag tgatcttatt tcattatggt gaaagttgga acctcttacg tgcccgatca
     1381 atcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa
     1441 aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca
     1501 aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt
     1561 ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgttcttct agtgtagccg
     1621 tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc
     1681 ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga
     1741 cgatagttac c
//
