LOCUS       sequence-208051-        1720 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  BTK Type 4 terminator for golden gate assembly .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 1720)
  AUTHORS   Leonard SP, Perutka J, Powell JE, Geng P, Richhart DD, Byrom M, Kar 
            S, Davies BW, Ellington AD, Moran NA, Barrick JE
  TITLE     Genetic Engineering of Bee Gut Microbiome Bacteria with a Toolkit 
            for Modular Assembly of Broad-Host-Range Plasmids.
  JOURNAL   ACS Synth Biol. 2018 May 18;7(5):1279-1290. doi: 
            10.1021/acssynbio.7b00399. Epub 2018 Apr 13.
  PUBMED    29608282
REFERENCE   2  (bases 1 to 1720)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acssynbio.7b00399"; journalName: "ACS Synth Biol"; date: 
            "2018-05-18- 18"; volume: "7"; issue: "5"; pages: "1279-1290"
COMMENT     Sequence Label: pBTK305
FEATURES             Location/Qualifiers
     source          1..1720
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     182..201
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      399..446
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     terminator      complement(467..501)
                     /label=lambda t0 terminator
                     /note="minimal transcription terminator from phage lambda 
                     (Scholtissek and Grosse, 1987)"
     CDS             complement(572..1231)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     1146..1165
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     rep_origin      join(1413..1720,1..281)
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg
       61 aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg
      121 cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag
      181 ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc
      241 gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct
      301 ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc
      361 ctgattctgt ggataaccgt agtcggtctc aatcctaact agcataaccc cttggggcct
      421 ctaaacgggt cttgaggggt tttttgctgg ctgtgagacc agaccaataa aaaacgcccg
      481 gcggcaaccg agcgttctga acaaatccag atggagttct gaggtcatta ctggatctat
      541 caacaggagt ccaagcgagc tcgatatcaa attacgcccc gccctgccac tcatcgcagt
      601 actgttgtaa ttcattaagc attctgccga catggaagcc atcacaaacg gcatgatgaa
      661 cctgaatcgc cagcggcatc agcaccttgt cgccttgcgt ataatatttg cccatggtga
      721 aaacgggggc gaagaagttg tccatattgg ccacgtttaa atcaaaactg gtgaaactca
      781 cccagggatt ggctgaaacg aaaaacatat tctcaataaa ccctttaggg aaataggcca
      841 ggttttcacc gtaacacgcc acatcttgcg aatatatgtg tagaaactgc cggaaatcgt
      901 cgtggtattc actccagagc gatgaaaacg tttcagtttg ctcatggaaa acggtgtaac
      961 aagggtgaac actatcccat atcaccagct caccgtcttt cattgccata cgaaattccg
     1021 gatgagcatt catcaggcgg gcaagaatgt gaataaaggc cggataaaac ttgtgcttat
     1081 ttttctttac ggtctttaaa aaggccgtaa tatccagctg aacggtctgg ttataggtac
     1141 attgagcaac tgactgaaat gcctcaaaat gttctttacg atgccattgg gatatatcaa
     1201 cggtggtata tccagtgatt tttttctcca ttttagcttc cttagctcct gaaaatctcg
     1261 ataactcaaa aaatacgccc ggtagtgatc ttatttcatt atggtgaaag ttggaacctc
     1321 ttacgtgccc gatcaatcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg
     1381 tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc
     1441 tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag
     1501 ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtt
     1561 cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac
     1621 ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc
     1681 gggttggact caagacgata gttaccggat aaggcgcagc
//
