LOCUS       sequence-208078-       12266 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  BTK assembled broad-host-range plasmid encoding inducible GFP driven
            by T7 polymerase.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12266)
  AUTHORS   Leonard SP, Perutka J, Powell JE, Geng P, Richhart DD, Byrom M, Kar 
            S, Davies BW, Ellington AD, Moran NA, Barrick JE
  TITLE     Genetic Engineering of Bee Gut Microbiome Bacteria with a Toolkit 
            for Modular Assembly of Broad-Host-Range Plasmids.
  JOURNAL   ACS Synth Biol. 2018 May 18;7(5):1279-1290. doi: 
            10.1021/acssynbio.7b00399. Epub 2018 Apr 13.
  PUBMED    29608282
REFERENCE   2  (bases 1 to 12266)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acssynbio.7b00399"; journalName: "ACS Synth Biol"; date: 
            "2018-05-18- 18"; volume: "7"; issue: "5"; pages: "1279-1290"
COMMENT     Sequence Label: pBTK550d
FEATURES             Location/Qualifiers
     source          1..12266
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     oriT            2013..2100
                     /label=RSF1010 oriT
                     /note="origin of transfer of the broad-host-range plasmid 
                     RSF1010 (Scholz et al., 1989)"
     rep_origin      2442..2836
                     /label=RSF1010 oriV
                     /note="replication origin of the broad-host-range plasmid 
                     RSF1010; requires the RSF1010 RepA/B/C proteins for 
                     replication (Scholz et al., 1989)"
     promoter        join(3338..3343,3344..3361,3362..3368)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    3376..3392
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             3397..3408
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     primer_bind     6273..6292
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        6273..6291
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    6296..6320
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     complement(7105..7123)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      7119..7166
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     terminator      complement(7429..7477)
                     /label=fd terminator
                     /note="central terminator from bacteriophage fd (Otsuka and
                     Kunisawa, 1982)"
     CDS             complement(7497..8579)
                     /codon_start=1
                     /gene="lacI"
                     /product="lac repressor"
                     /label=lacI
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAEL
                     NYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGV
                     EACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSH
                     EDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSA
                     MSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSC
                     YIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPR
                     ALADSLMQLARQVSRLESGQ"
     primer_bind     8541..8560
                     /label=LacI-R
                     /note="LacI, reverse primer"
     CDS             complement(8710..9501)
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     CDS             complement(9881..10732)
                     /codon_start=1
                     /gene="repC"
                     /product="replication protein C of the broad-host-range 
                     plasmid RSF1010 (Scholz et al., 1989)"
                     /label=RSF1010 RepC
                     /translation="MVKPKNKHSLSHVRHDPAHCLAPGLFRALKRGERKRSKLDVTYDY
                     GDGKRIEFSGPEPLGADDLRILQGLVAMAGPNGLVLGPEPKTEGGRQLRLFLEPKWEAV
                     TAECHVVKGSYRALAKEIGAEVDSGGALKHIQDCIERLWKVSIIAQNGRKRQGFRLLSE
                     YASDEADGRLYVALNPLIAQAVMGGGQHVRISMDEVRALDSETARLLHQRLCGWIDPGK
                     TGKASIDTLCGYVWPSEASGSTMRKRRKRVREALPELVALGWTVTEFAAGKYDITRPKA
                     AG"
     CDS             complement(10719..11558)
                     /codon_start=1
                     /gene="repA"
                     /product="replication protein A of the broad-host-range 
                     plasmid RSF1010 (Scholz et al., 1989)"
                     /label=RSF1010 RepA
                     /translation="MATHKPINILEAFAAAPPPLDYVLPNMVAGTVGALVSPGGAGKSM
                     LALQLAAQIAGGPDLLEVGELPTGPVIYLPAEDPPTAIHHRLHALGAHLSAEERQAVAD
                     GLLIQPLIGSLPNIMAPEWFDGLKRAAEGRRLMVLDTLRRFHIEEENASGPMAQVIGRM
                     EAIAADTGCSIVFLHHASKGAAMMGAGDQQQASRGSSVLVDNIRWQSYLSSMTSAEAEE
                     WGVDDDQRRFFVRFGVSKANYGAPFADRWFRRHDGGVLKPAVLERQRKSKGVPRGEA"
     CDS             complement(join(12069..12266,1..774))
                     /codon_start=1
                     /gene="repB"
                     /product="replication protein B of the broad-host-range 
                     plasmid RSF1010 (Scholz et al., 1989)"
                     /label=RSF1010 RepB
                     /translation="MKNDRTLQAIGRQLKAMGCERFDIGVRDAPTGQMMNREWSAAEVL
                     QNTPWLKRMNAQGNDVYIRPAEQERHGLVLVDDLSEFDLDDMKAEGREPALVVETSPKN
                     YQAWVKVADAAGGELRGQIARTLASEYDADPASADSRHYGRLAGFTNRKDKHTTRAGYQ
                     PWVLLRESKGKTATAGPALVQQAGQQIEQAQRQQEKARRLASLELPERQLSRHRRTALD
                     EYRSEMAGLVKRFGHDLSKCDFIAAQKLASRGRSAEEIGKAMAEASPALAERKPGHEAD
                     YIERTVSKVMGLPSVQLARAELARAPAPRQRGMDRGGPDFSM"
ORIGIN
        1 ctcggcactg cggccccggc tggccagctt ctgcgcggcg ataaagtcgc acttgctgag
       61 gtcatgaccg aagcgcttga ccagcccggc catctcgctg cggtactcgt ccagcgccgt
      121 gcgccggtgg cggctaagct gccgctcggg cagttcgagg ctggccagcc tgcgggcctt
      181 ctcctgctgc cgctgggcct gctcgatctg ctggccagcc tgctgcacca gcgccgggcc
      241 agcggtggcg gtcttgccct tggattcacg cagcagcacc cacggctgat aaccggcgcg
      301 ggtggtgtgc ttgtccttgc ggttggtgaa gcccgccaag cggccatagt ggcggctgtc
      361 ggcgctggcc gggtcggcgt cgtactcgct ggccagcgtc cgggcaatct gcccccgaag
      421 ttcaccgcct gcggcgtcgg ccaccttgac ccatgcctga tagttcttcg ggctggtttc
      481 cactaccagg gcaggctccc ggccctcggc tttcatgtca tccaggtcaa actcgctgag
      541 gtcgtccacc agcaccagac catgccgctc ctgctcggcg ggcctgatat acacgtcatt
      601 gccctgggca ttcatccgct tgagccatgg cgtgttctgg agcacttcgg cggctgacca
      661 ttcccggttc atcatctggc cggtgggtgc gtccctgacg ccgatatcga agcgctcaca
      721 gcccatggcc ttgagctgtc ggcctatggc ctgcaaagtc ctgtcgttct tcatcgggcc
      781 accaagcgca gccagatcga gccgtcctcg gttgtcagtg gcgtcaggtc gagcaagagc
      841 aacgatgcga tcagcagcac caccgtaggc atcatggaag ccagcatcac ggttagccat
      901 agcttccagt gccacccccg cgacgcgctc cgggcgctct gcgcggcgct gctcacctcg
      961 gcggctacct cccgcaactc tttggccagc tccacccatg ccgcccctgt ctggcgctgg
     1021 gctttcagcc actccgccgc ctgcgcctcg ctggcctgct tggtctggct catgacctgc
     1081 cgggcttcgt cggccagtgt cgccatgctc tgggccagcg gttcgatctg ctccgctaac
     1141 tcgttgatgc ctctggattt cttcactctg tcgattgcgt tcatggtcta ttgcctcccg
     1201 gtattcctgt aagtcgatga tctgggcgtt ggcggtgtcg atgttcaggg ccacgtctgc
     1261 ccggtcggtg cggatgcccc ggccttccat ctccaccacg ttcggcccca ggtgaacacc
     1321 gggcaggcgc tcgatgccct gcgcctcaag tgttctgtgg tcaatgcggg cgtcgtggcc
     1381 agcccgctct aatgcccggt tggcatggtc ggcccatgcc tcgcgggtct gctcaagcca
     1441 tgccttgggc ttgagcgctt cggtcttctg tgccccgccc ttctccgggg tcttgccgtt
     1501 gtaccgcttg aaccactgag cggcgggccg ctcgatgccg tcattgatcc gctcggagat
     1561 catcaggtgg cagtgcgggt tctcgccgcc accggcatgg atggccagcg tatacggcag
     1621 gcgctcggca ccggtcaggt gctgggcgaa ctcggacgcc agcgccttct gctggtcgag
     1681 ggtcagctcg accggcaggg caaattcgac ctccttgaac agccgcccat tggcgcgttc
     1741 atacaggtcg gcagcatccc agtagtcggc gggccgctcg acgaactccg gcatgtgccc
     1801 ggattcggcg tgcaagactt catccatgtc gcgggcatac ttgccttcgc gctggatgta
     1861 gtcggccttg gccctggccg attggccgcc cgacctgctg ccggttttcg ccgtaaggtg
     1921 ataaatcgcc atgctgcctc gctgttgctt ttgcttttcg gctccatgca atggccctcg
     1981 gagagcgcac cgcccgaagg gtggccgtta ggccagtttc tcgaagagaa accggtaagt
     2041 gcgccctccc ctacaaagta gggtcgggat tgccgccgct gtgcctccat gatagcctac
     2101 gagacagcac attaacaatg gggtgtcaag atggttaagg ggagcaacaa ggcggcggat
     2161 cggctggcca agctcgaaga acaacgagcg cgaatcaatg ccgaaattca gcgggagcgg
     2221 gcaagggaac agcagcaaga gcgcaagaac gaaacaaggc gcaaggtgct ggtgggggcc
     2281 atgattttgg ccaaggtgaa cagcagcgag tggccggagg atcggctcat ggcggcaatg
     2341 gatgcgtacc ttgaacgcga ccacgaccgc gccttgttcg gtctgccgcc acgccagaag
     2401 gatgagccgg gctgaatgat cgaccgagac aggccctgcg gggctgcaca cgcgccccca
     2461 cccttcgggt agggggaaag gccgctaaag cggctaaaag cgctccagcg tatttctgcg
     2521 gggtttggtg tggggtttag cgggctttgc ccgcctttcc ccctgccgcg cagcggtggg
     2581 gcggtgtgta gcctagcgca gcgaatagac cagctatccg gcctctggcc gggcatattg
     2641 ggcaagggca gcagcgcccc acaagggcgc tgataaccgc gcctagtgga ttattcttag
     2701 ataatcatgg atggattttt ccaacacccc gccagccccc gcccctgctg ggtttgcagg
     2761 tttgggggcg tgacagttat tgcaggggtt cgtgacagtt attgcagggg ggcgtgacag
     2821 ttattgcagg ggttcgtgac agttagtacg ggagtgacgg gcactggctg gcaatgtcta
     2881 gcaacggcag gcatttcggc tgagggtaaa agaactttcc gctaagcgat agactgtatg
     2941 taaacacagt attgcaagga cgcggaacat gcctcatgtg gcggccagga cggccagccg
     3001 ggatcgggat actggtcgtt accagagcca ccgacccgag caaacccttc tctatcagat
     3061 cgttgacgag tattacccgg cattcgctgc gcttatggca gagcagggaa aggaattgcc
     3121 gggctatgtg caacgggaat ttgaagaatt tctccaatgc gggcggctgg agcatggctt
     3181 tctacgggtt cgctgcgagt cttgccacgc cgagcacctg gtcgctttca gaaatcaatc
     3241 taaagtatat atgagtaaac ttggtctgac aggcccctga attcgcatct agacagggtc
     3301 ttgctctgtc accctcaaga caacctgccc ctcaacgttt acactttatg cttccggctc
     3361 gtatgttgtg tggaattgtg agcggataac aattttaaag aggagaaatt aactatgaac
     3421 acgattaaca tcgctaagaa cgacttctct gacatcgaac tggctgctat cccgttcaac
     3481 actctggctg accattacgg tgagcgttta gctcgcgaac agttggccct tgagcatgag
     3541 tcttacgaga tgggtgaagc acgcttccgc aagatgtttg agcgtcaact taaagctggt
     3601 gaggttgcgg ataacgctgc cgccaagcct ctcatcacta ccctactccc taagatgatt
     3661 gcacgcatca acgactggtt tgaggaagtg aaagctaagc gcggcaagcg cccgacagcc
     3721 ttccagttcc tgcaagaaat caagccggaa gccgtagcgt acatcaccat taagaccact
     3781 ctggcttgcc taaccagtgc tgacaataca accgttcagg ctgtagcaag cgcaatcggt
     3841 cgggccattg aggacgaggc tcgcttcggt cgtatccgtg accttgaagc taagcacttc
     3901 aagaaaaacg ttgaggaaca actcaacaag cgcgtagggc acgtctacaa gaaagcattt
     3961 atgcaagttg tcgaggctga catgctctct aagggtctac tcggtggcga ggcgtggtct
     4021 tcgtggcata aggaagactc tattcatgta ggagtacgct gcatcgagat gctcattgag
     4081 tcaaccggaa tggttagctt acaccgccaa aatgctggcg tagtaggtca agactctgag
     4141 actatcgaac tcgcacctga atacgctgag gctatcgcaa cccgtgcagg tgcgctggct
     4201 ggcatctctc cgatgttcca accttgcgta gttcctccta agccgtggac tggcattact
     4261 ggtggtggct attgggctaa cggtcgtcgt cctctggcgc tggtgcgtac tcacagtaag
     4321 aaagcactga tgcgctacga agacgtttac atgcctgagg tgtacaaagc gattaacatt
     4381 gcgcaaaaca ccgcatggaa aatcaacaag aaagtcctag cggtcgccaa cgtaatcacc
     4441 aagtggaagc attgtccggt cgaggacatc cctgcgattg agcgtgaaga actcccgatg
     4501 aaaccggaag acatcgacat gaatcctgag gctctcaccg cgtggaaacg tgctgccgct
     4561 gctgtgtacc gcaaggacaa ggctcgcaag tctcgccgta tcagccttga gttcatgctt
     4621 gagcaagcca ataagtttgc taaccataag gccatctggt tcccttacaa catggactgg
     4681 cgcggtcgtg tttacgctgt gtcaatgttc aacccgcaag gtaacgatat gaccaaagga
     4741 ctgcttacgc tggcgaaagg taaaccaatc ggtaaggaag gttactactg gctgaaaatc
     4801 cacggtgcaa actgtgcggg tgtcgataag gttccgttcc ctgagcgcat caagttcatt
     4861 gaggaaaacc acgagaacat catggcttgc gctaagtctc cactggagaa cacttggtgg
     4921 gctgagcaag attctccgtt ctgcttcctt gcgttctgct ttgagtacgc tggggtacag
     4981 caccacggcc tgagctataa ctgctccctt ccgctggcgt ttgacgggtc ttgctctggc
     5041 atccagcact tctccgcgat gctccgagat gaggtaggtg gtcgcgcggt taacttgctt
     5101 cctagtgaaa ccgttcagga catctacggg attgttgcta agaaagtcaa cgagattcta
     5161 caagcagacg caatcaatgg gaccgataac gaagtagtta ccgtgaccga tgagaacact
     5221 ggtgaaatct ctgagaaagt caagctgggc actaaggcac tggctggtca atggctggct
     5281 tacggtgtta ctcgcagtgt gactaagcgt tcagtcatga cgctggctta cgggtccaaa
     5341 gagttcggct tccgtcaaca agtgctggaa gataccattc agccagctat tgattccggc
     5401 aagggtctga tgttcactca gccgaatcag gctgctggat acatggctaa gctgatttgg
     5461 gaatctgtga gcgtgacggt ggtagctgcg gttgaagcaa tgaactggct taagtctgct
     5521 gctaagctgc tggctgctga ggtcaaagat aagaagactg gagagattct tcgcaagcgt
     5581 tgcgctgtgc attgggtaac tcctgatggt ttccctgtgt ggcaggaata caagaagcct
     5641 attcagacgc gcttgaacct gatgttcctc ggtcagttcc gcttacagcc taccattaac
     5701 accaacaaag atagcgagat tgatgcacac aaacaggagt ctggtatcgc tcctaacttt
     5761 gtacacagcc aagacggtag ccaccttcgt aagactgtag tgtgggcaca cgagaagtac
     5821 ggaatcgaat cttttgcact gattcacgac tccttcggta ccattccggc tgacgctgcg
     5881 aacctgttca aagcagtgcg cgaaactatg gttgacacat atgagtcttg tgatgtactg
     5941 gctgatttct acgaccagtt cgctgaccag ttgcacgagt ctcaattgga caaaatgcca
     6001 gcacttccgg ctaaaggtaa cttgaacctc cgtgacatct tagagtcgga cttcgcgttc
     6061 gcgtaaatcc gtaatcgtta atccgcaaat aacgtaaaaa cccgcttcgg cgggtttttt
     6121 tatgggggga gtttagggaa agagcatttg tcagctgctg aactggccga taattgcaga
     6181 cgaacgtcgg tcagtttcac ctgatttacg taaaaacccg cttcggcggg tttttgcttt
     6241 tggaggggca gaaagatgaa tgactgtcaa attaatacga ctcactatag ggagaggaat
     6301 tgtgagcgga taacaattcc atacagaaac agaggagata tatgagtaaa ggagaagagc
     6361 ttttcacagg agttgtccca atcctcgtgg aattagacgg tgatgttaat gggcacaagt
     6421 tctctgtcag tggagagggt gaaggcgacg caacatatgg caagctgacc cttaaattta
     6481 tttgcaccac gggtaaacta cctgttccat ggccaacact ggtcactacg ttcgggtatg
     6541 gggttcagtg ctttgcgcgc tacccagatc acatgaaaca gcacgacttt ttcaagagtg
     6601 caatgcccga aggctatgta caggagagaa ccatcttttt taaggatgac ggcaactata
     6661 agacacgcgc cgaagtgaag ttcgagggtg atacccttgt taatagaatc gagttaaagg
     6721 gtattgactt taaggaagat ggaaatattt taggccacaa actggaatat aactataact
     6781 cccataatgt gtacattatg gccgacaagc aaaagaacgg tatcaaggtt aacttcaaga
     6841 tcagacacaa cattgaggat ggaagcgttc aactagccga ccattaccaa caaaacaccc
     6901 caattggcga tgggcctgtg ctgttaccag acaaccatta cctgtccact caatctgccc
     6961 tttcgaaaga tcccaacgaa aagcgcgacc acatggtcct tcttgagttt gtcacggctg
     7021 ctgggattac acacggcatg gatgaactat acaaataaat ccctgctaac aaagcccgaa
     7081 aggaagctga gttggctgct gccaccgctg agcaataact agcataaccc cttggggcct
     7141 ctaaacgggt cttgaggggt tttttgctga aaggaggaac tatatgctgc catccctgaa
     7201 aaccacccca tagtcaggat cacaaggacc ccagcagtta cagagatgtt acgaaccccc
     7261 aggacatccg agaatgcgag gcgatggagg gtacaaccgg gagagtgttc accgacaaac
     7321 aacagataaa acaaaaggcc cagtcttccg actgagcctt ttgttttatt tgatgtctgg
     7381 cagttccccg ccgctatctg atcatcaaat aataatttct tcatgttgaa aatctccaaa
     7441 aaaaaaggct ccaaaaggag cctttaattg tatcggttta tcagcttgct tttacatcac
     7501 tgcccgcttt ccagtcggga aacctgtcgt gccagctgca ttaatgaatc ggccaacgcg
     7561 cggggagagg cggtttgcgt attgggcgcc agggtggttt ttcttttcac cagtgacacc
     7621 ggcaacagct gattgccctt caccgcctgg ccctgagaga gttgcagcaa gcggtccacg
     7681 ctggtttgcc ccagcaggcg aaaatcctgt ttgatggtgg ttaacggcgg gatataacat
     7741 gagctgtctt cggtatcgtc gtatcccact accgagatat ccgcaccaac gcgcagcccg
     7801 gactcggtaa tggcgcgcat tgcgcccagc gccatctgat cgttggcaac cagcatcgca
     7861 gtgggaacga tgccctcatt cagcatttgc atggtttgtt gaaaaccgga catggcactc
     7921 cagtcgcctt cccgttccgc tatcggctga atttgattgc gagtgagata tttatgccag
     7981 ccagccagac gcagacgcgc cgagacagaa cttaatgggc ccgctaacag cgcgatttgc
     8041 tggtgaccca atgcgaccag atgctccacg cccagtcgcg taccgtcttc atgggagaaa
     8101 ataatactgt tgatgggtgt ctggtcagag acatcaagaa ataacgccgg aacattagtg
     8161 caggcagctt ccacagcaat ggcatcctgg tcatccagcg gatagttaat gatcagccca
     8221 ctgacgcgtt gcgcgagaag attgtgcacc gccgctttac aggcttcgac gccgcttcgt
     8281 tctaccatcg acaccaccac gctggcaccc agttgatcgg cgcgagattt aatcgccgcg
     8341 acaatttgcg acggcgcgtg cagggccaga ctggaggtgg caacgccaat cagcaacgac
     8401 tgtttgcccg ccagttgttg tgccacgcgg ttgggaatgt aattcagctc cgccatcgcc
     8461 gcttccactt tttcccgcgt tttcgcagaa acgtggctgg cctggttcac cacgcgggaa
     8521 acggtctgat aagagacacc ggcatactct gcgacatcgt ataacgttac tggtttcatg
     8581 agtacctctc ctgtatcttg tgtacttgcg taccgtaata tctcctctgt ttctgtataa
     8641 cagtactatg tgattatacc agccccctca ctacatgtca agaataaact gccaaagccg
     8701 agcggccgct tatttgccga ctaccttggt gatctcgcct ttcacgtagt ggacaaattc
     8761 ttccaactga tctgcgcgcg aggccaagcg atcttcttct tgtccaagat aagcctgtct
     8821 agcttcaagt atgacgggct gatactgggc cggcaggcgc tccattgccc agtcggcagc
     8881 gacatccttc ggcgcgattt tgccggttac tgcgctgtac caaatgcggg acaacgtaag
     8941 cactacattt cgctcatcgc cagcccagtc gggcggcgag ttccatagcg ttaaggtttc
     9001 atttagcgcc tcaaatagat cctgttcagg aaccggatca aagagttcct ccgccgctgg
     9061 acctaccaag gcaacgctat gttctcttgc ttttgtcagc aagatagcca gatcaatgtc
     9121 gatcgtggct ggctcgaaga tacccgcaag aatgtcattg cgctgccatt ctccaaattg
     9181 cagttcgcgc ttagctggat aacgccacgg aatgatgtcg tcgtgcacaa caatggtgac
     9241 ttctacagcg cggagaatct cgctctctcc aggggaagcc gaagtttcca aaaggtcgtt
     9301 gatcaaagct cgccgcgttg tttcatcaag ccttacggtc accgtaacca gcaaatcaat
     9361 atcactgtgt ggcttcaggc cgccatccac tgcggagccg tacaaatgta cggccagcaa
     9421 cgtcggttcg agatggcgct cgatgacgcc aactacctct gatagttgag tcgatacttc
     9481 ggcgatcacc gcttccctca taatgtttaa ctttgtttta gggcgactgc cctgctgcgt
     9541 aacatcgttg ctgctccata acatcaaaca tcgacccacg gcgtaacgcg cttgctgctt
     9601 ggatgcccga ggcatagact gtaccccaaa aaaacagtca taacaagcca tgaaaaccgc
     9661 cactgcgccg ttaccaccgc tgcgttcggt caaggttctg gaccagttgc gtgagcgcat
     9721 acgctacttg cattacagct tacgaaccga acaggcttat gtccactggg ttcgtgcctt
     9781 catccgtttc cacggtgtgc gtcacccggc aaccttgggt agcagcgaag tcgaggcatt
     9841 tctgtcctgg ctggtcatga ccaaaatccc ttaacgtgag tcagcctgcc gccttgggcc
     9901 gggtgatgtc gtacttgccc gccgcgaact cggttaccgt ccagcccagc gcgaccagct
     9961 ccggcaacgc ctcgcgcacc cgcttgcggc gcttgcgcat ggtcgaacca ctggcctctg
    10021 acggccagac atagccgcac aaggtatcta tggaagcctt gccggttttg ccggggtcga
    10081 tccagccaca cagccgctgg tgcagcaggc gggcggtttc gctgtccagc gcccgcacct
    10141 cgtccatgct gatgcgcaca tgctggccgc cacccatgac ggcctgcgcg atcaaggggt
    10201 tcagggccac gtacaggcgc ccgtccgcct cgtcgctggc gtactccgac agcagccgaa
    10261 acccctgccg cttgcggcca ttctgggcga tgatggatac cttccaaagg cgctcgatgc
    10321 agtcctgtat gtgcttgagc gccccaccac tatcgacctc tgccccgatt tcctttgcca
    10381 gcgcccgata gctacctttg accacatggc attcagcggt gacggcctcc cacttgggtt
    10441 ccaggaacag ccggagctgc cgtccgcctt cggtcttggg ttccgggcca agcactaggc
    10501 cattaggccc agccatggcc accagccctt gcaggatgcg cagatcatca gcgcccagcg
    10561 gctccgggcc gctgaactcg atccgcttgc cgtcgccgta gtcatacgtc acgtccagct
    10621 tgctgcgctt gcgctcgccc cgcttgaggg cacggaacag gccgggggcc agacagtgcg
    10681 ccgggtcgtg ccggacgtgg ctgaggctgt gcttgttctt aggcttcacc acggggcacc
    10741 cccttgctct tgcgctgcct ctccagcacg gcgggcttga gcaccccgcc gtcatgccgc
    10801 ctgaaccacc gatcagcgaa cggtgcgcca tagttggcct tgctcacacc gaagcggacg
    10861 aagaaccggc gctggtcgtc gtccacaccc cattcctcgg cctcggcgct ggtcatgctc
    10921 gacaggtagg actgccagcg gatgttatcg accagtaccg agctgccccg gctggcctgc
    10981 tgctggtcgc ctgcgcccat catggccgcg cccttgctgg catggtgcag gaacacgata
    11041 gagcacccgg tatcggcggc gatggcctcc atgcgaccga tgacctgggc catggggccg
    11101 ctggcgtttt cttcctcgat gtggaaccgg cgcagcgtgt ccagcaccat caggcggcgg
    11161 ccctcggcgg cgcgcttgag gccgtcgaac cactccgggg ccatgatgtt gggcaggctg
    11221 ccgatcagcg gctggatcag caggccgtca gccacggctt gccgttcctc ggcgctgagg
    11281 tgcgccccaa gggcgtgcag gcggtgatga atggcggtgg gcgggtcttc ggcgggcagg
    11341 tagatcaccg ggccggtggg cagttcgccc acctccagca gatccggccc gcctgcaatc
    11401 tgtgcggcca gttgcagggc cagcatggat ttaccggcac caccgggcga caccagcgcc
    11461 ccgaccgtac cggccaccat gttgggcaaa acgtagtcca gcggtggcgg cgctgctgcg
    11521 aacgcctcca gaatattgat aggcttatgg gtagccattg attgcctcct ttgcaggcag
    11581 ttggtggtta ggcgctggcg gggtcactac ccccgccctg cgccgctctg agttcttcca
    11641 ggcactcgcg cagcgcctcg tattcgtcgt cggtcagcca gaacttgcgc tgacgcatcc
    11701 ctttggcctt catgcgctcg gcatatcgcg cttggcgtac agcgtcaggg ctggccagca
    11761 ggtcgccggt ctgcttgtcc ttttggtctt tcatatcagt caccgagaaa cttgccgggg
    11821 ccgaaaggct tgtcttcgcg gaacaaggac aaggtgcagc cgtcaaggtt aaggctggcc
    11881 atatcagcga ctgaaaagcg gccagcctcg gccttgtttg acgtataacc aaagccaccg
    11941 ggcaaccaat agcccttgtc acttttgatc aggtagaccg accctgaagc gcttttttcg
    12001 tattccataa aacccccttc tgtgcgtgag tactcatagt ataacaggcg tgagtaccaa
    12061 cgcaagcact acatgctgaa atctggcccg cccctgtcca tgcctcgctg gcggggtgcc
    12121 ggtgcccgtg ccagctcggc ccgcgcaagc tggacgctgg gcagacccat gaccttgctg
    12181 acggtgcgct cgatgtaatc cgcttcgtgg ccgggcttgc gctctgccag cgctgggctg
    12241 gcctcggcca tggccttgcc gatttc
//
