LOCUS       sequence-208079-        9492 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  BTK assembled broad-host-range plasmid encoding inducible GFP driven
            CP25 promoter with lac operator. Also encodes LacI..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9492)
  AUTHORS   Leonard SP, Perutka J, Powell JE, Geng P, Richhart DD, Byrom M, Kar 
            S, Davies BW, Ellington AD, Moran NA, Barrick JE
  TITLE     Genetic Engineering of Bee Gut Microbiome Bacteria with a Toolkit 
            for Modular Assembly of Broad-Host-Range Plasmids.
  JOURNAL   ACS Synth Biol. 2018 May 18;7(5):1279-1290. doi: 
            10.1021/acssynbio.7b00399. Epub 2018 Apr 13.
  PUBMED    29608282
REFERENCE   2  (bases 1 to 9492)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acssynbio.7b00399"; journalName: "ACS Synth Biol"; date: 
            "2018-05-18- 18"; volume: "7"; issue: "5"; pages: "1279-1290"
COMMENT     Sequence Label: pBTK552
FEATURES             Location/Qualifiers
     source          1..9492
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     oriT            2204..2291
                     /label=RSF1010 oriT
                     /note="origin of transfer of the broad-host-range plasmid 
                     RSF1010 (Scholz et al., 1989)"
     rep_origin      2633..3027
                     /label=RSF1010 oriV
                     /note="replication origin of the broad-host-range plasmid 
                     RSF1010; requires the RSF1010 RepA/B/C proteins for 
                     replication (Scholz et al., 1989)"
     protein_bind    3784..3808
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     terminator      complement(4846..4894)
                     /label=fd terminator
                     /note="central terminator from bacteriophage fd (Otsuka and
                     Kunisawa, 1982)"
     CDS             complement(4914..5996)
                     /codon_start=1
                     /gene="lacI"
                     /product="lac repressor"
                     /label=lacI
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAEL
                     NYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGV
                     EACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSH
                     EDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSA
                     MSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSC
                     YIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPR
                     ALADSLMQLARQVSRLESGQ"
     primer_bind     5958..5977
                     /label=LacI-R
                     /note="LacI, reverse primer"
     CDS             complement(6127..6918)
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     CDS             complement(7298..8149)
                     /codon_start=1
                     /gene="repC"
                     /product="replication protein C of the broad-host-range 
                     plasmid RSF1010 (Scholz et al., 1989)"
                     /label=RSF1010 RepC
                     /translation="MVKPKNKHSLSHVRHDPAHCLAPGLFRALKRGERKRSKLDVTYDY
                     GDGKRIEFSGPEPLGADDLRILQGLVAMAGPNGLVLGPEPKTEGGRQLRLFLEPKWEAV
                     TAECHVVKGSYRALAKEIGAEVDSGGALKHIQDCIERLWKVSIIAQNGRKRQGFRLLSE
                     YASDEADGRLYVALNPLIAQAVMGGGQHVRISMDEVRALDSETARLLHQRLCGWIDPGK
                     TGKASIDTLCGYVWPSEASGSTMRKRRKRVREALPELVALGWTVTEFAAGKYDITRPKA
                     AG"
     CDS             complement(8136..8975)
                     /codon_start=1
                     /gene="repA"
                     /product="replication protein A of the broad-host-range 
                     plasmid RSF1010 (Scholz et al., 1989)"
                     /label=RSF1010 RepA
                     /translation="MATHKPINILEAFAAAPPPLDYVLPNMVAGTVGALVSPGGAGKSM
                     LALQLAAQIAGGPDLLEVGELPTGPVIYLPAEDPPTAIHHRLHALGAHLSAEERQAVAD
                     GLLIQPLIGSLPNIMAPEWFDGLKRAAEGRRLMVLDTLRRFHIEEENASGPMAQVIGRM
                     EAIAADTGCSIVFLHHASKGAAMMGAGDQQQASRGSSVLVDNIRWQSYLSSMTSAEAEE
                     WGVDDDQRRFFVRFGVSKANYGAPFADRWFRRHDGGVLKPAVLERQRKSKGVPRGEA"
     CDS             complement(join(9486..9492,1..965))
                     /codon_start=1
                     /gene="repB"
                     /product="replication protein B of the broad-host-range 
                     plasmid RSF1010 (Scholz et al., 1989)"
                     /label=RSF1010 RepB
                     /translation="MKNDRTLQAIGRQLKAMGCERFDIGVRDAPTGQMMNREWSAAEVL
                     QNTPWLKRMNAQGNDVYIRPAEQERHGLVLVDDLSEFDLDDMKAEGREPALVVETSPKN
                     YQAWVKVADAAGGELRGQIARTLASEYDADPASADSRHYGRLAGFTNRKDKHTTRAGYQ
                     PWVLLRESKGKTATAGPALVQQAGQQIEQAQRQQEKARRLASLELPERQLSRHRRTALD
                     EYRSEMAGLVKRFGHDLSKCDFIAAQKLASRGRSAEEIGKAMAEASPALAERKPGHEAD
                     YIERTVSKVMGLPSVQLARAELARAPAPRQRGMDRGGPDFSM"
ORIGIN
        1 ctgaaatctg gcccgcccct gtccatgcct cgctggcggg gtgccggtgc ccgtgccagc
       61 tcggcccgcg caagctggac gctgggcaga cccatgacct tgctgacggt gcgctcgatg
      121 taatccgctt cgtggccggg cttgcgctct gccagcgctg ggctggcctc ggccatggcc
      181 ttgccgattt cctcggcact gcggccccgg ctggccagct tctgcgcggc gataaagtcg
      241 cacttgctga ggtcatgacc gaagcgcttg accagcccgg ccatctcgct gcggtactcg
      301 tccagcgccg tgcgccggtg gcggctaagc tgccgctcgg gcagttcgag gctggccagc
      361 ctgcgggcct tctcctgctg ccgctgggcc tgctcgatct gctggccagc ctgctgcacc
      421 agcgccgggc cagcggtggc ggtcttgccc ttggattcac gcagcagcac ccacggctga
      481 taaccggcgc gggtggtgtg cttgtccttg cggttggtga agcccgccaa gcggccatag
      541 tggcggctgt cggcgctggc cgggtcggcg tcgtactcgc tggccagcgt ccgggcaatc
      601 tgcccccgaa gttcaccgcc tgcggcgtcg gccaccttga cccatgcctg atagttcttc
      661 gggctggttt ccactaccag ggcaggctcc cggccctcgg ctttcatgtc atccaggtca
      721 aactcgctga ggtcgtccac cagcaccaga ccatgccgct cctgctcggc gggcctgata
      781 tacacgtcat tgccctgggc attcatccgc ttgagccatg gcgtgttctg gagcacttcg
      841 gcggctgacc attcccggtt catcatctgg ccggtgggtg cgtccctgac gccgatatcg
      901 aagcgctcac agcccatggc cttgagctgt cggcctatgg cctgcaaagt cctgtcgttc
      961 ttcatcgggc caccaagcgc agccagatcg agccgtcctc ggttgtcagt ggcgtcaggt
     1021 cgagcaagag caacgatgcg atcagcagca ccaccgtagg catcatggaa gccagcatca
     1081 cggttagcca tagcttccag tgccaccccc gcgacgcgct ccgggcgctc tgcgcggcgc
     1141 tgctcacctc ggcggctacc tcccgcaact ctttggccag ctccacccat gccgcccctg
     1201 tctggcgctg ggctttcagc cactccgccg cctgcgcctc gctggcctgc ttggtctggc
     1261 tcatgacctg ccgggcttcg tcggccagtg tcgccatgct ctgggccagc ggttcgatct
     1321 gctccgctaa ctcgttgatg cctctggatt tcttcactct gtcgattgcg ttcatggtct
     1381 attgcctccc ggtattcctg taagtcgatg atctgggcgt tggcggtgtc gatgttcagg
     1441 gccacgtctg cccggtcggt gcggatgccc cggccttcca tctccaccac gttcggcccc
     1501 aggtgaacac cgggcaggcg ctcgatgccc tgcgcctcaa gtgttctgtg gtcaatgcgg
     1561 gcgtcgtggc cagcccgctc taatgcccgg ttggcatggt cggcccatgc ctcgcgggtc
     1621 tgctcaagcc atgccttggg cttgagcgct tcggtcttct gtgccccgcc cttctccggg
     1681 gtcttgccgt tgtaccgctt gaaccactga gcggcgggcc gctcgatgcc gtcattgatc
     1741 cgctcggaga tcatcaggtg gcagtgcggg ttctcgccgc caccggcatg gatggccagc
     1801 gtatacggca ggcgctcggc accggtcagg tgctgggcga actcggacgc cagcgccttc
     1861 tgctggtcga gggtcagctc gaccggcagg gcaaattcga cctccttgaa cagccgccca
     1921 ttggcgcgtt catacaggtc ggcagcatcc cagtagtcgg cgggccgctc gacgaactcc
     1981 ggcatgtgcc cggattcggc gtgcaagact tcatccatgt cgcgggcata cttgccttcg
     2041 cgctggatgt agtcggcctt ggccctggcc gattggccgc ccgacctgct gccggttttc
     2101 gccgtaaggt gataaatcgc catgctgcct cgctgttgct tttgcttttc ggctccatgc
     2161 aatggccctc ggagagcgca ccgcccgaag ggtggccgtt aggccagttt ctcgaagaga
     2221 aaccggtaag tgcgccctcc cctacaaagt agggtcggga ttgccgccgc tgtgcctcca
     2281 tgatagccta cgagacagca cattaacaat ggggtgtcaa gatggttaag gggagcaaca
     2341 aggcggcgga tcggctggcc aagctcgaag aacaacgagc gcgaatcaat gccgaaattc
     2401 agcgggagcg ggcaagggaa cagcagcaag agcgcaagaa cgaaacaagg cgcaaggtgc
     2461 tggtgggggc catgattttg gccaaggtga acagcagcga gtggccggag gatcggctca
     2521 tggcggcaat ggatgcgtac cttgaacgcg accacgaccg cgccttgttc ggtctgccgc
     2581 cacgccagaa ggatgagccg ggctgaatga tcgaccgaga caggccctgc ggggctgcac
     2641 acgcgccccc acccttcggg tagggggaaa ggccgctaaa gcggctaaaa gcgctccagc
     2701 gtatttctgc ggggtttggt gtggggttta gcgggctttg cccgcctttc cccctgccgc
     2761 gcagcggtgg ggcggtgtgt agcctagcgc agcgaataga ccagctatcc ggcctctggc
     2821 cgggcatatt gggcaagggc agcagcgccc cacaagggcg ctgataaccg cgcctagtgg
     2881 attattctta gataatcatg gatggatttt tccaacaccc cgccagcccc cgcccctgct
     2941 gggtttgcag gtttgggggc gtgacagtta ttgcaggggt tcgtgacagt tattgcaggg
     3001 gggcgtgaca gttattgcag gggttcgtga cagttagtac gggagtgacg ggcactggct
     3061 ggcaatgtct agcaacggca ggcatttcgg ctgagggtaa aagaactttc cgctaagcga
     3121 tagactgtat gtaaacacag tattgcaagg acgcggaaca tgcctcatgt ggcggccagg
     3181 acggccagcc gggatcggga tactggtcgt taccagagcc accgacccga gcaaaccctt
     3241 ctctatcaga tcgttgacga gtattacccg gcattcgctg cgcttatggc agagcaggga
     3301 aaggaattgc cgggctatgt gcaacgggaa tttgaagaat ttctccaatg cgggcggctg
     3361 gagcatggct ttctacgggt tcgctgcgag tcttgccacg ccgagcacct ggtcgctttc
     3421 agaaatcaat ctaaagtata tatgagtaaa cttggtctga caggcccctg aattcgcatc
     3481 tagactgatg agacgtggta gagccacaaa cagccggtac aagcaacgat ctccaggacc
     3541 atctgaatca tgcgcggatg acacgaactc acgacggcga tcacagacat taacccacag
     3601 tacagacact gcgacaacgt ggcaattcgt cgcaatacaa cgtcggtcag tttcacctga
     3661 tttacgtaaa aacccgcttc ggcgggtttt tgcttttgga ggggcagaaa gatgaatgac
     3721 tgtcctttgg cagtttattc ttgacatgta gtgagggggc tggtataatc acatagtact
     3781 gttggaattg tgagcggata acaattccat acagaaacag aggagatatt acatatgagt
     3841 aaaggagaag agcttttcac aggagttgtc ccaatcctcg tggaattaga cggtgatgtt
     3901 aatgggcaca agttctctgt cagtggagag ggtgaaggcg acgcaacata tggcaagctg
     3961 acccttaaat ttatttgcac cacgggtaaa ctacctgttc catggccaac actggtcact
     4021 acgttcgggt atggggttca gtgctttgcg cgctacccag atcacatgaa acagcacgac
     4081 tttttcaaga gtgcaatgcc cgaaggctat gtacaggaga gaaccatctt ttttaaggat
     4141 gacggcaact ataagacacg cgccgaagtg aagttcgagg gtgataccct tgttaataga
     4201 atcgagttaa agggtattga ctttaaggaa gatggaaata ttttaggcca caaactggaa
     4261 tataactata actcccataa tgtgtacatt atggccgaca agcaaaagaa cggtatcaag
     4321 gttaacttca agatcagaca caacattgag gatggaagcg ttcaactagc cgaccattac
     4381 caacaaaaca ccccaattgg cgatgggcct gtgctgttac cagacaacca ttacctgtcc
     4441 actcaatctg ccctttcgaa agatcccaac gaaaagcgcg accacatggt ccttcttgag
     4501 tttgtcacgg ctgctgggat tacacacggc atggatgaac tatacaaata aatccgtaat
     4561 cgttaatccg caaataacgt aaaaacccgc ttcggcgggt ttttttatgg ggggagttta
     4621 gggaaagagc atttgtcagc tgcgtctcaa gcagttacag agatgttacg aacccccagg
     4681 acatccgaga atgcgaggcg atggagggta caaccgggag agtgttcacc gacaaacaac
     4741 agataaaaca aaaggcccag tcttccgact gagccttttg ttttatttga tgtctggcag
     4801 ttccccgccg ctatctgatc atcaaataat aatttcttca tgttgaaaat ctccaaaaaa
     4861 aaaggctcca aaaggagcct ttaattgtat cggtttatca gcttgctttt acatcactgc
     4921 ccgctttcca gtcgggaaac ctgtcgtgcc agctgcatta atgaatcggc caacgcgcgg
     4981 ggagaggcgg tttgcgtatt gggcgccagg gtggtttttc ttttcaccag tgacaccggc
     5041 aacagctgat tgcccttcac cgcctggccc tgagagagtt gcagcaagcg gtccacgctg
     5101 gtttgcccca gcaggcgaaa atcctgtttg atggtggtta acggcgggat ataacatgag
     5161 ctgtcttcgg tatcgtcgta tcccactacc gagatatccg caccaacgcg cagcccggac
     5221 tcggtaatgg cgcgcattgc gcccagcgcc atctgatcgt tggcaaccag catcgcagtg
     5281 ggaacgatgc cctcattcag catttgcatg gtttgttgaa aaccggacat ggcactccag
     5341 tcgccttccc gttccgctat cggctgaatt tgattgcgag tgagatattt atgccagcca
     5401 gccagacgca gacgcgccga gacagaactt aatgggcccg ctaacagcgc gatttgctgg
     5461 tgacccaatg cgaccagatg ctccacgccc agtcgcgtac cgtcttcatg ggagaaaata
     5521 atactgttga tgggtgtctg gtcagagaca tcaagaaata acgccggaac attagtgcag
     5581 gcagcttcca cagcaatggc atcctggtca tccagcggat agttaatgat cagcccactg
     5641 acgcgttgcg cgagaagatt gtgcaccgcc gctttacagg cttcgacgcc gcttcgttct
     5701 accatcgaca ccaccacgct ggcacccagt tgatcggcgc gagatttaat cgccgcgaca
     5761 atttgcgacg gcgcgtgcag ggccagactg gaggtggcaa cgccaatcag caacgactgt
     5821 ttgcccgcca gttgttgtgc cacgcggttg ggaatgtaat tcagctccgc catcgccgct
     5881 tccacttttt cccgcgtttt cgcagaaacg tggctggcct ggttcaccac gcgggaaacg
     5941 gtctgataag agacaccggc atactctgcg acatcgtata acgttactgg tttcatgagt
     6001 acctctcctg tatcttgtgt acttgcgtac cgtaatatct cctctgtttc tgtataacag
     6061 tactatgtga ttataccagc cccctcacta catgtcaaga ataaactgcc aaagccgagc
     6121 ggccgcttat ttgccgacta ccttggtgat ctcgcctttc acgtagtgga caaattcttc
     6181 caactgatct gcgcgcgagg ccaagcgatc ttcttcttgt ccaagataag cctgtctagc
     6241 ttcaagtatg acgggctgat actgggccgg caggcgctcc attgcccagt cggcagcgac
     6301 atccttcggc gcgattttgc cggttactgc gctgtaccaa atgcgggaca acgtaagcac
     6361 tacatttcgc tcatcgccag cccagtcggg cggcgagttc catagcgtta aggtttcatt
     6421 tagcgcctca aatagatcct gttcaggaac cggatcaaag agttcctccg ccgctggacc
     6481 taccaaggca acgctatgtt ctcttgcttt tgtcagcaag atagccagat caatgtcgat
     6541 cgtggctggc tcgaagatac ccgcaagaat gtcattgcgc tgccattctc caaattgcag
     6601 ttcgcgctta gctggataac gccacggaat gatgtcgtcg tgcacaacaa tggtgacttc
     6661 tacagcgcgg agaatctcgc tctctccagg ggaagccgaa gtttccaaaa ggtcgttgat
     6721 caaagctcgc cgcgttgttt catcaagcct tacggtcacc gtaaccagca aatcaatatc
     6781 actgtgtggc ttcaggccgc catccactgc ggagccgtac aaatgtacgg ccagcaacgt
     6841 cggttcgaga tggcgctcga tgacgccaac tacctctgat agttgagtcg atacttcggc
     6901 gatcaccgct tccctcataa tgtttaactt tgttttaggg cgactgccct gctgcgtaac
     6961 atcgttgctg ctccataaca tcaaacatcg acccacggcg taacgcgctt gctgcttgga
     7021 tgcccgaggc atagactgta ccccaaaaaa acagtcataa caagccatga aaaccgccac
     7081 tgcgccgtta ccaccgctgc gttcggtcaa ggttctggac cagttgcgtg agcgcatacg
     7141 ctacttgcat tacagcttac gaaccgaaca ggcttatgtc cactgggttc gtgccttcat
     7201 ccgtttccac ggtgtgcgtc acccggcaac cttgggtagc agcgaagtcg aggcatttct
     7261 gtcctggctg gtcatgacca aaatccctta acgtgagtca gcctgccgcc ttgggccggg
     7321 tgatgtcgta cttgcccgcc gcgaactcgg ttaccgtcca gcccagcgcg accagctccg
     7381 gcaacgcctc gcgcacccgc ttgcggcgct tgcgcatggt cgaaccactg gcctctgacg
     7441 gccagacata gccgcacaag gtatctatgg aagccttgcc ggttttgccg gggtcgatcc
     7501 agccacacag ccgctggtgc agcaggcggg cggtttcgct gtccagcgcc cgcacctcgt
     7561 ccatgctgat gcgcacatgc tggccgccac ccatgacggc ctgcgcgatc aaggggttca
     7621 gggccacgta caggcgcccg tccgcctcgt cgctggcgta ctccgacagc agccgaaacc
     7681 cctgccgctt gcggccattc tgggcgatga tggatacctt ccaaaggcgc tcgatgcagt
     7741 cctgtatgtg cttgagcgcc ccaccactat cgacctctgc cccgatttcc tttgccagcg
     7801 cccgatagct acctttgacc acatggcatt cagcggtgac ggcctcccac ttgggttcca
     7861 ggaacagccg gagctgccgt ccgccttcgg tcttgggttc cgggccaagc actaggccat
     7921 taggcccagc catggccacc agcccttgca ggatgcgcag atcatcagcg cccagcggct
     7981 ccgggccgct gaactcgatc cgcttgccgt cgccgtagtc atacgtcacg tccagcttgc
     8041 tgcgcttgcg ctcgccccgc ttgagggcac ggaacaggcc gggggccaga cagtgcgccg
     8101 ggtcgtgccg gacgtggctg aggctgtgct tgttcttagg cttcaccacg gggcaccccc
     8161 ttgctcttgc gctgcctctc cagcacggcg ggcttgagca ccccgccgtc atgccgcctg
     8221 aaccaccgat cagcgaacgg tgcgccatag ttggccttgc tcacaccgaa gcggacgaag
     8281 aaccggcgct ggtcgtcgtc cacaccccat tcctcggcct cggcgctggt catgctcgac
     8341 aggtaggact gccagcggat gttatcgacc agtaccgagc tgccccggct ggcctgctgc
     8401 tggtcgcctg cgcccatcat ggccgcgccc ttgctggcat ggtgcaggaa cacgatagag
     8461 cacccggtat cggcggcgat ggcctccatg cgaccgatga cctgggccat ggggccgctg
     8521 gcgttttctt cctcgatgtg gaaccggcgc agcgtgtcca gcaccatcag gcggcggccc
     8581 tcggcggcgc gcttgaggcc gtcgaaccac tccggggcca tgatgttggg caggctgccg
     8641 atcagcggct ggatcagcag gccgtcagcc acggcttgcc gttcctcggc gctgaggtgc
     8701 gccccaaggg cgtgcaggcg gtgatgaatg gcggtgggcg ggtcttcggc gggcaggtag
     8761 atcaccgggc cggtgggcag ttcgcccacc tccagcagat ccggcccgcc tgcaatctgt
     8821 gcggccagtt gcagggccag catggattta ccggcaccac cgggcgacac cagcgccccg
     8881 accgtaccgg ccaccatgtt gggcaaaacg tagtccagcg gtggcggcgc tgctgcgaac
     8941 gcctccagaa tattgatagg cttatgggta gccattgatt gcctcctttg caggcagttg
     9001 gtggttaggc gctggcgggg tcactacccc cgccctgcgc cgctctgagt tcttccaggc
     9061 actcgcgcag cgcctcgtat tcgtcgtcgg tcagccagaa cttgcgctga cgcatccctt
     9121 tggccttcat gcgctcggca tatcgcgctt ggcgtacagc gtcagggctg gccagcaggt
     9181 cgccggtctg cttgtccttt tggtctttca tatcagtcac cgagaaactt gccggggccg
     9241 aaaggcttgt cttcgcggaa caaggacaag gtgcagccgt caaggttaag gctggccata
     9301 tcagcgactg aaaagcggcc agcctcggcc ttgtttgacg tataaccaaa gccaccgggc
     9361 aaccaatagc ccttgtcact tttgatcagg tagaccgacc ctgaagcgct tttttcgtat
     9421 tccataaaac ccccttctgt gcgtgagtac tcatagtata acaggcgtga gtaccaacgc
     9481 aagcactaca tg
//
