LOCUS       sequence-208083-       19157 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Express human PC1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 19157)
  AUTHORS   Lin CC, Kurashige M, Liu Y, Terabayashi T, Ishimoto Y, Wang T, 
            Choudhary V, Hobbs R, Liu LK, Lee PH, Outeda P, Zhou F, Restifo NP, 
            Watnick T, Kawano H, Horie S, Prinz W, Xu H, Menezes LF, Germino GG
  TITLE     A cleavage product of Polycystin-1 is a mitochondrial matrix protein
            that affects mitochondria morphology and function when 
            heterologously expressed.
  JOURNAL   Sci Rep. 2018 Feb 9;8(1):2743. doi: 10.1038/s41598-018-20856-6.
  PUBMED    29426897
REFERENCE   2  (bases 1 to 19157)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Rep."; 
            date: "2018-02-9"; volume: "8(1):2743. doi"; pages: " 
            10.1038/s41598-018-20856-6"
COMMENT     Sequence Label: AF20-double-tag
FEATURES             Location/Qualifiers
     source          1..19157
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        138..517
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        518..721
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     718..742
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     intron          857..989
                     /label=chimeric intron
                     /note="chimera between introns from human beta-globin and 
                     immunoglobulin heavy chain genes"
     primer_bind     972..991
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     promoter        1034..1052
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     intron          1083..1655
                     /label=beta-globin intron
                     /note="intron from rabbit beta-globin gene"
     primer_bind     1663..1682
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     protein_bind    1713..1737
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             1815..2522
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(1960..1978)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     2228..2247
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     CDS             2523..2543
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             2544..2585
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             join(15435..15437,15438..15440,15441..16151)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(15435..15437,15438..15440,15441..16151)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(15480..15501)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(15741..15760)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     16088..16109
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             16152..16172
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             16173..16217
                     /codon_start=1
                     /product="affinity and epitope tag derived from pancreatic 
                     ribonuclease A"
                     /label=S-Tag
                     /translation="KETAAAKFERQHMDS"
     polyA_signal    16260..16308
                     /note="synthetic polyadenylation signal"
     rep_origin      16545..17000
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(16632..16651)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     16842..16863
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(17067..17086)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     17186..17208
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(17246..17264)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        17332..17436
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(17437..17505,17506..18297)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(17655..17674)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      18468..19056
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     18957..18976
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 tcaatattgg ccattagcca tattattcat tggttatata gcataaatca atattggcta
       61 ttggccattg catacgttgt atctatatca taatatgtac atttatattg gctcatgtcc
      121 aatatgaccg ccatgttggc attgattatt gactagttat taatagtaat caattacggg
      181 gtcattagtt catagcccat atatggagtt ccgcgttaca taacttacgg taaatggccc
      241 gcctggctga ccgcccaacg acccccgccc attgacgtca ataatgacgt atgttcccat
      301 agtaacgcca atagggactt tccattgacg tcaatgggtg gagtatttac ggtaaactgc
      361 ccacttggca gtacatcaag tgtatcatat gccaagtccg ccccctattg acgtcaatga
      421 cggtaaatgg cccgcctggc attatgccca gtacatgacc ttacgggact ttcctacttg
      481 gcagtacatc tacgtattag tcatcgctat taccatggtg atgcggtttt ggcagtacac
      541 caatgggcgt ggatagcggt ttgactcacg gggatttcca agtctccacc ccattgacgt
      601 caatgggagt ttgttttggc accaaaatca acgggacttt ccaaaatgtc gtaataaccc
      661 cgccccgttg acgcaaatgg gcggtaggcg tgtacggtgg gaggtctata taagcagagc
      721 tcgtttagtg aaccgtcaga tcactagaag ctttattgcg gtagtttatc acagttaaat
      781 tgctaacgca gtcagtgctt ctgacacaac agtctcgaac ttaagctgca gaagttggtc
      841 gtgaggcact gggcaggtaa gtatcaaggt tacaagacag gtttaaggag accaatagaa
      901 actgggcttg tcgagacaga gaagactctt gcgtttctga taggcaccta ttggtcttac
      961 tgacatccac tttgcctttc tctccacagg tgtccactcc cagttcaatt acagctctta
     1021 aggctagagt acttaatacg actcactata ggctagtctg gatcgatcct gagaacttca
     1081 gggtgagttt ggggaccctt gattgttctt tctttttcgc tattgtaaaa ttcatgttat
     1141 atggaggggg caaagttttc agggtgttgt ttagaatggg aagatgtccc ttgtatcacc
     1201 atggaccctc atgataattt tgtttctttc actttctact ctgttgacaa ccattgtctc
     1261 ctcttatttt cttttcattt tctgtaactt tttcgttaaa ctttagcttg catttgtaac
     1321 gaatttttaa attcactttt gtttatttgt cagattgtaa gtactttctc taatcacttt
     1381 tttttcaagg caatcagggt atattatatt gtacttcagc acagttttag agaacaattg
     1441 ttataattaa atgataaggt agaatatttc tgcatataaa ttctggctgg cgtggaaata
     1501 ttcttattgg tagaaacaac tacatcctgg tcatcatcct gcctttctct ttatggttac
     1561 aatgatatac actgtttgag atgaggataa aatactctga gtccaaaccg ggcccctctg
     1621 ctaaccatgt tcatgccttc ttctttttcc tacagctcct gggcaacgtg ctggttattg
     1681 tgctgtctca tcattttggc aaagaattcg gggcaacttt gtacaaaaaa gttgatatat
     1741 ccaccatgcc gcccgccgcg cccgcccgcc tggcgctggc cctgggcctg ggcctgtggc
     1801 tcggggcgct ggcgatggtt agtaagggcg aggaggataa catggccatc atcaaggagt
     1861 tcatgcgctt caaggtgcac atggagggct ccgtgaacgg ccacgagttc gagatcgagg
     1921 gcgagggcga gggccgcccc tacgagggca cccagaccgc caagctgaag gtgaccaagg
     1981 gtggccccct gcccttcgcc tgggacatcc tgtcccctca gttcatgtac ggctccaagg
     2041 cctacgtgaa gcaccccgcc gacatccccg actacttgaa gctgtccttc cccgagggct
     2101 tcaagtggga gcgcgtgatg aacttcgagg acggcggcgt ggtgaccgtg acccaggact
     2161 cctccctgca ggacggcgag ttcatctaca aggtgaagct gcgcggcacc aacttcccct
     2221 ccgacggccc cgtaatgcag aagaagacca tgggctggga ggcctcctcc gagcggatgt
     2281 accccgagga cggcgccctg aagggcgaga tcaagcagag gctgaagctg aaggacggcg
     2341 gccactacga cgctgaggtc aagaccacct acaaggccaa gaagcccgtg cagctgcccg
     2401 gcgcctacaa cgtcaacatc aagttggaca tcacctccca caacgaggac tacaccatcg
     2461 tggaacagta cgaacgcgcc gagggccgcc actccaccgg cggcatggac gagctgtaca
     2521 aggagaatct ttattttcag ggcggtaagc ctatccctaa ccctctcctc ggtctcgatt
     2581 ctacggatat cgggggcccc gggcgcggct gcgggccctg cgagcccccc tgcctctgcg
     2641 gcccagcgcc cggcgccgcc tgccgcgtca actgctcggg ccgcgggctg cggacgctcg
     2701 gtcccgcgct gcgcatcccc gcggacgcca cagcgctaga cgtctcccac aacctgctcc
     2761 gggcgctgga cgttgggctc ctggcgaacc tctcggcgct ggcagagctg gatataagca
     2821 acaacaagat ttctacgtta gaagaaggaa tatttgctaa tttatttaat ttaagtgaaa
     2881 taaacctgag tgggaacccg tttgagtgtg actgtggcct ggcgtggctg ccgcgatggg
     2941 cggaggagca gcaggtgcgg gtggtgcagc ccgaggcagc cacgtgtgct gggcctggct
     3001 ccctggctgg ccagcctctg cttggcatcc ccttgctgga cagtggctgt ggtgaggagt
     3061 atgtcgcctg cctccctgac aacagctcag gcaccgtggc agcagtgtcc ttttcagctg
     3121 cccacgaagg cctgcttcag ccagaggcct gcagcgcctt ctgcttctcc accggccagg
     3181 gcctcgcagc cctctcggag cagggctggt gcctgtgtgg ggcggcccag ccctccagtg
     3241 cctcctttgc ctgcctgtcc ctctgctccg gccccccgcc acctcctgcc cccacctgta
     3301 ggggccccac cctcctccag cacgtcttcc ctgcctcccc aggggccacc ctggtggggc
     3361 cccacggacc tctggcctct ggccagctag cagccttcca catcgctgcc ccgctccctg
     3421 tcactgccac acgctgggac ttcggagacg gctccgccga ggtggatgcc gctgggccgg
     3481 ctgcctcgca tcgctatgtg ctgcctgggc gctatcacgt gacggccgtg ctggccctgg
     3541 gggccggctc agccctgctg gggacagacg tgcaggtgga agcggcacct gccgccctgg
     3601 agctcgtgtg cccgtcctcg gtgcagagtg acgagagcct tgacctcagc atccagaacc
     3661 gcggtggttc aggcctggag gccgcctaca gcatcgtggc cctgggcgag gagccggccc
     3721 gagcggtgca cccgctctgc ccctcggaca cggagatctt ccctggcaac gggcactgct
     3781 accgcctggt ggtggagaag gcggcctggc tgcaggcgca ggagcagtgt caggcctggg
     3841 ccggggccgc cctggcaatg gtggacagtc ccgccgtgca gcgcttcctg gtctctcggg
     3901 tcaccaggag cctagacgtg tggatcggct tctcgactgt gcagggggtg gaggtgggcc
     3961 cagcgccgca gggcgaggcc ttcagcctgg agagctgcca gaactggctg cccggggagc
     4021 cacacccagc cacagccgag cactgcgtcc ggctcgggcc caccgggtgg tgtaacaccg
     4081 acctgtgctc agcgccgcac agctacgtct gcgagctgca gcccggaggc ccagtgcagg
     4141 atgccgagaa cctcctcgtg ggagcgccca gtggggacct gcagggaccc ctgacgcctc
     4201 tggcacagca ggacggcctc tcagccccgc acgagcccgt ggaggtcatg gtattcccgg
     4261 gcctgcgtct gagccgtgaa gccttcctca ccacggccga atttgggacc caggagctcc
     4321 ggcggcccgc ccagctgcgg ctgcaggtgt accggctcct cagcacagca gggaccccgg
     4381 agaacggcag cgagcctgag agcaggtccc cggacaacag gacccagctg gcccccgcgt
     4441 gcatgccagg gggacgctgg tgccctggag ccaacatctg cttgccgctg gacgcctcct
     4501 gccaccccca ggcctgcgcc aatggctgca cgtcagggcc agggctaccc ggggccccct
     4561 atgcgctatg gagagagttc ctcttctccg ttcccgcggg gccccccgcg cagtactcgg
     4621 tcaccctcca cggccaggat gtcctcatgc tccctggtga cctcgttggc ttgcagcacg
     4681 acgctggccc tggcgccctc ctgcactgct cgccggctcc cggccaccct ggtccccagg
     4741 ccccgtacct ctccgccaac gcctcgtcat ggctgcccca cttgccagcc cagctggagg
     4801 gcacttgggc ctgccctgcc tgtgccctgc ggctgcttgc agccacggaa cagctcaccg
     4861 tgctgctggg cttgaggccc aaccctggac tgcggctgcc tgggcgctat gaggtccggg
     4921 cagaggtggg caatggcgtg tccaggcaca acctctcctg cagctttgac gtggtctccc
     4981 cagtggctgg gctgcgggtc atctaccctg ccccccgcga cggccgcctc tacgtgccca
     5041 ccaacggctc agccttggtg ctccaggtgg actctggtgc caacgccacg gccacggctc
     5101 gctggcctgg gggcagtgtc agcgcccgct ttgagaatgt ctgccctgcc ctggtggcca
     5161 ccttcgtgcc cggctgcccc tgggagacca acgataccct gttctcagtg gtagcactgc
     5221 cgtggctcag tgagggggag cacgtggtgg acgtggtggt ggaaaacagc gccagccggg
     5281 ccaacctcag cctgcgggtg acggcggagg agcccatctg tggcctccgc gccacgccca
     5341 gccccgaggc ccgtgtactg cagggagtcc tagtgaggta cagccccgtg gtggaggccg
     5401 gctcggacat ggtcttccgg tggaccatca acgacaagca gtccctgacc ttccagaacg
     5461 tggtcttcaa tgtcatttat cagagcgcgg cggtcttcaa gctctcactg acggcctcca
     5521 accacgtgag caacgtcacc gtgaactaca acgtaaccgt ggagcggatg aacaggatgc
     5581 agggtctgca ggtctccaca gtgccggccg tgctgtcccc caatgccacg ctggcactga
     5641 cggcgggcgt gctggtggac tcggccgtgg aggtggcctt cctgtggacc tttggggatg
     5701 gggagcaggc cctccaccag ttccagcctc cgtacaacga gtccttcccg gttccagacc
     5761 cctcggtggc ccaggtgctg gtggagcaca atgtcatgca cacctacgct gccccaggtg
     5821 agtacctcct gaccgtgctg gcatctaatg ccttcgagaa cctgacgcag caggtgcctg
     5881 tgagcgtgcg cgcctccctg ccctccgtgg ctgtgggtgt gagtgacggc gtcctggtgg
     5941 ccggccgggc cgtcaccttc tacccgcacc cgctgccctc gcctgggggt gttctttaca
     6001 cgtgggactt cggggacggc tcccctgtcc tgacccagag ccagccggct gccaaccaca
     6061 cctatgcctc gaggggcacc taccacgtgc gcctggaggt caacaacacg gtgagcggtg
     6121 cggcggccca ggcggatgtg cgcgtctttg aggagctccg cggactcagc gtggacatga
     6181 gcctggccgt ggagcagggc gcccccgtgg tggtcagcgc cgcggtgcag acgggcgaca
     6241 acatcacgtg gaccttcgac atgggggacg gcaccgtgct gtcgggcccg gaggcaacag
     6301 tggagcatgt gtacctgcgg gcacagaact gcacagtgac cgtgggtgcg gccagccccg
     6361 ccggccacct ggcccggagc ctgcacgtgc tggtcttcgt cctggaggtg ctgcgcgttg
     6421 aacccgccgc ctgcatcccc acgcagcctg acgcgcggct cacggcctac gtcaccggga
     6481 acccggccca ctacctcttc gactggacct tcggggatgg ctcctccaac acgaccgtgc
     6541 gggggtgccc gacggtgaca cacaacttca cgcggagcgg cacgttcccc ctggcgctgg
     6601 tgctgtccag ccgcgtgaac agggcgcatt acttcaccag catctgcgtg gagccagagg
     6661 tgggcaacgt caccctgcag ccagagaggc agtttgtgca gctcggggac gaggcctggc
     6721 tggtggcatg tgcctggccc ccgttcccct accgctacac ctgggacttt ggcaccgagg
     6781 aagccgcccc cacccgtgcc aggggccctg aggtgacgtt catctaccga gacccaggct
     6841 cctatcttgt gacagtcacc gcgtccaaca acatctctgc tgccaatgac tcagccctgg
     6901 tggaggtgca ggagcccgtg ctggtcacca gcatcaaggt caatggctcc cttgggctgg
     6961 agctgcagca gccgtacctg ttctctgctg tgggccgtgg gcgccccgcc agctacctgt
     7021 gggatctggg ggacggtggg tggctcgagg gtccggaggt cacccacgct tacaacagca
     7081 caggtgactt caccgttagg gtggccggct ggaatgaggt gagccgcagc gaggcctggc
     7141 tcaatgtgac ggtgaagcgg cgcgtgcggg ggctcgtcgt caatgcaagc cgcacggtgg
     7201 tgcccctgaa tgggagcgtg agcttcagca cgtcgctgga ggccggcagt gatgtgcgct
     7261 attcctgggt gctctgtgac cgctgcacgc ccatccctgg gggtcctacc atctcttaca
     7321 ccttccgctc cgtgggcacc ttcaatatca tcgtcacggc tgagaacgag gtgggctccg
     7381 cccaggacag catcttcgtc tatgtcctgc agctcataga ggggctgcag gtggtgggcg
     7441 gtggccgcta cttccccacc aaccacacgg tacagctgca ggccgtggtt agggatggca
     7501 ccaacgtctc ctacagctgg actgcctgga gggacagggg cccggccctg gccggcagcg
     7561 gcaaaggctt ctcgctcacc gtgctcgagg ccggcaccta ccatgtgcag ctgcgggcca
     7621 ccaacatgct gggcagcgcc tgggccgact gcaccatgga cttcgtggag cctgtggggt
     7681 ggctgatggt ggccgcctcc ccgaacccag ctgccgtcaa cacaagcgtc accctcagtg
     7741 ccgagctggc tggtggcagt ggtgtcgtat acacttggtc cttggaggag gggctgagct
     7801 gggagacctc cgagccattt accacccata gcttccccac acccggcctg cacttggtca
     7861 ccatgacggc agggaacccg ctgggctcag ccaacgccac cgtggaagtg gatgtgcagg
     7921 tgcctgtgag tggcctcagc atcagggcca gcgagcccgg aggcagcttc gtggcggccg
     7981 ggtcctctgt gcccttttgg gggcagctgg ccacgggcac caatgtgagc tggtgctggg
     8041 ctgtgcccgg cggcagcagc aagcgtggcc ctcatgtcac catggtcttc ccggatgctg
     8101 gcaccttctc catccggctc aatgcctcca acgcagtcag ctgggtctca gccacgtaca
     8161 acctcacggc ggaggagccc atcgtgggcc tggtgctgtg ggccagcagc aaggtggtgg
     8221 cgcccgggca gctggtccat tttcagatcc tgctggctgc cggctcagct gtcaccttcc
     8281 gcctgcaggt cggcggggcc aaccccgagg tgctccccgg gccccgtttc tcccacagct
     8341 tcccccgcgt cggagaccac gtggtgagcg tgcggggcaa aaaccacgtg agctgggccc
     8401 aggcgcaggt gcgcatcgtg gtgctggagg ccgtgagtgg gctgcaggtg cccaactgct
     8461 gcgagcctgg catcgccacg ggcactgaga ggaacttcac agcccgcgtg cagcgcggct
     8521 ctcgggtcgc ctacgcctgg tacttctcgc tgcagaaggt ccagggcgac tcgctggtca
     8581 tcctgtcggg ccgcgacgtc acctacacgc ccgtggccgc ggggctgttg gagatccagg
     8641 tgcgcgcctt caacgccctg ggcagtgaga accgcacgct ggtgctggag gttcaggacg
     8701 ccgtccagta tgtggccctg cagagcggcc cctgcttcac caaccgctcg gcgcagtttg
     8761 aggccgccac cagccccagc ccccggcgtg tggcctacca ctgggacttt ggggatgggt
     8821 cgccagggca ggacacagat gagcccaggg ccgagcactc ctacctgagg cctggggact
     8881 accgcgtgca ggtgaacgcc tccaacctgg tgagcttctt cgtggcgcag gccacggtga
     8941 ccgtccaggt gctggcctgc cgggagccgg aggtggacgt ggtcctgccc ctgcaggtgc
     9001 tgatgcggcg atcacagcgc aactacttgg aggcccacgt tgacctgcgc gactgcgtca
     9061 cctaccagac tgagtaccgc tgggaggtgt atcgcaccgc cagctgccag cggccggggc
     9121 gcccagcgcg tgtggccctg cccggcgtgg acgtgagccg gcctcggctg gtgctgccgc
     9181 ggctggcgct gcctgtgggg cactactgct ttgtgtttgt cgtgtcattt ggggacacgc
     9241 cactgacaca gagcatccag gccaatgtga cggtggcccc cgagcgcctg gtgcccatca
     9301 ttgagggtgg ctcataccgc gtgtggtcag acacacggga cctggtgctg gatgggagcg
     9361 agtcctacga ccccaacctg gaggacggcg accagacgcc gctcagtttc cactgggcct
     9421 gtgtggcttc gacacagagg gaggctggcg ggtgtgcgct gaactttggg ccccgcggga
     9481 gcagcacggt caccattcca cgggagcggc tggcggctgg cgtggagtac accttcagcc
     9541 tgaccgtgtg gaaggccggc cgcaaggagg aggccaccaa ccagacggtg ctgatccgga
     9601 gtggccgggt gcccattgtg tccttggagt gtgtgtcctg caaggcacag gccgtgtacg
     9661 aagtgagccg cagctcctac gtgtacttgg agggccgctg cctcaattgc agcagcggct
     9721 ccaagcgagg gcggtgggct gcacgtacgt tcagcaacaa gacgctggtg ctggatgaga
     9781 ccaccacatc cacgggcagt gcaggcatgc gactggtgct gcggcggggc gtgctgcggg
     9841 acggcgaggg atacaccttc acgctcacgg tgctgggccg ctctggcgag gaggagggct
     9901 gcgcctccat ccgcctgtcc cccaaccgcc cgccgctggg gggctcttgc cgcctcttcc
     9961 cactgggcgc tgtgcacgcc ctcaccacca aggtgcactt cgaatgcacg ggctggcatg
    10021 acgcggagga tgctggcgcc ccgctggtgt acgccctgct gctgcggcgc tgtcgccagg
    10081 gccactgcga ggagttctgt gtctacaagg gcagcctctc cagctacgga gccgtgctgc
    10141 ccccgggttt caggccacac ttcgaggtgg gcctggccgt ggtggtgcag gaccagctgg
    10201 gagccgctgt ggtcgccctc aacaggtctt tggccatcac cctcccagag cccaacggca
    10261 gcgcaacggg gctcacagtc tggctgcacg ggctcaccgc tagtgtgctc ccagggctgc
    10321 tgcggcaggc cgatccccag cacgtcatcg agtactcgtt ggccctggtc accgtgctga
    10381 acgagtacga gcgggccctg gacgtggcgg cagagcccaa gcacgagcgg cagcaccgag
    10441 cccagatacg caagaacatc acggagactc tggtgtccct gagggtccac actgtggatg
    10501 acatccagca gatcgctgct gcgctggccc agtgcatggg gcccagcagg gagctcgtat
    10561 gccgctcgtg cctgaagcag acgctgcaca agctggaggc catgatgctc atcctgcagg
    10621 cagagaccac cgcgggcacc gtgacgccca ccgccatcgg agacagcatc ctcaacatca
    10681 caggagacct catccacctg gccagctcgg acgtgcgggc accacagccc tcagagctgg
    10741 gagccgagtc accatctcgg atggtggcgt cccaggccta caacctgacc tctgccctca
    10801 tgcgcatcct catgcgctcc cgcgtgctca acgaggagcc cctgacgctg gcgggcgagg
    10861 agatcgtggc ccagggcaag cgctcggacc cgcggagcct gctgtgctat ggcggcgccc
    10921 cagggcctgg ctgccacttc tccatccccg aggctttcag cggggccctg gccaacctca
    10981 gtgacgtggt gcagctcatc tttctggtgg actccaatcc ctttcccttt ggctatatca
    11041 gcaactacac cgtctccacc aaggtggcct cgatggcatt ccagacacag gccggcgccc
    11101 agatccccat cgagcggctg gcctcagagc gcgccatcac cgtgaaggtg cccaacaact
    11161 cggactgggc tgcccggggc caccgcagct ccgccaactc cgccaactcc gttgtggtcc
    11221 agccccaggc ctccgtcggt gctgtggtca ccctggacag cagcaaccct gcggccgggc
    11281 tgcatctgca gctcaactat acgctgctgg acggccacta cctgtctgag gaacctgagc
    11341 cctacctggc agtctaccta cactcggagc cccggcccaa tgagcacaac tgctcggcta
    11401 gcaggaggat ccgcccagag tcactccagg gtgctgacca ccggccctac accttcttca
    11461 tttccccggg gagcagagac ccagcgggga gttaccatct gaacctctcc agccacttcc
    11521 gctggtcggc gctgcaggtg tccgtgggcc tgtacacgtc cctgtgccag tacttcagcg
    11581 aggaggacat ggtgtggcgg acagaggggc tgctgcccct ggaggagacc tcgccccgcc
    11641 aggccgtctg cctcacccgc cacctcaccg ccttcggcgc cagcctcttc gtgcccccaa
    11701 gccatgtccg ctttgtgttt cctgagccga cagcggatgt aaactacatc gtcatgctga
    11761 catgtgctgt gtgcctggtg acctacatgg tcatggccgc catcctgcac aagctggacc
    11821 agttggatgc cagccggggc cgcgccatcc ctttctgtgg gcagcggggc cgcttcaagt
    11881 acgagatcct cgtcaagaca ggctggggcc ggggctcagg taccacggcc cacgtgggca
    11941 tcatgctgta tggggtggac agccggagcg gccaccggca cctggacggc gacagagcct
    12001 tccaccgcaa cagcctggac atcttccgga tcgccacccc gcacagcctg ggtagcgtgt
    12061 ggaagatccg agtgtggcac gacaacaaag ggctcagccc tgcctggttc ctgcagcacg
    12121 tcatcgtcag ggacctgcag acggcacgca gcgccttctt cctggtcaat gactggcttt
    12181 cggtggagac ggaggccaac gggggcctgg tggagaagga ggtgctggcc gcgagcgacg
    12241 cagccctttt gcgcttccgg cgcctgctgg tggctgagct gcagcgtggc ttctttgaca
    12301 agcacatctg gctctccata tgggaccggc cgcctcgtag ccgtttcact cgcatccaga
    12361 gggccacctg ctgcgttctc ctcatctgcc tcttcctggg cgccaacgcc gtgtggtacg
    12421 gggctgttgg cgactctgcc tacagcacgg ggcatgtgtc caggctgagc ccgctgagcg
    12481 tcgacacagt cgctgttggc ctggtgtcca gcgtggttgt ctatcccgtc tacctggcca
    12541 tcctttttct cttccggatg tcccggagca aggtggctgg gagcccgagc cccacacctg
    12601 ccgggcagca ggtgctggac atcgacagct gcctggactc gtccgtgctg gacagctcct
    12661 tcctcacgtt ctcaggcctc cacgctgagg cctttgttgg acagatgaag agtgacttgt
    12721 ttctggatga ttctaagagt ctggtgtgct ggccctccgg cgagggaacg ctcagttggc
    12781 cggacctgct cagtgacccg tccattgtgg gtagcaatct gcggcagctg gcacggggcc
    12841 aggcgggcca tgggctgggc ccagaggagg acggcttctc cctggccagc ccctactcgc
    12901 ctgccaaatc cttctcagca tcagatgaag acctgatcca gcaggtcctt gccgaggggg
    12961 tcagcagccc agcccctacc caagacaccc acatggaaac ggacctgctc agcagcctgt
    13021 ccagcactcc tggggagaag acagagacgc tggcgctgca gaggctgggg gagctggggc
    13081 cacccagccc aggcctgaac tgggaacagc cccaggcagc gaggctgtcc aggacaggac
    13141 tggtggaggg tctgcggaag cgcctgctgc cggcctggtg tgcctccctg gcccacgggc
    13201 tcagcctgct cctggtggct gtggctgtgg ctgtctcagg gtgggtgggt gcgagcttcc
    13261 ccccgggcgt gagtgttgcg tggctcctgt ccagcagcgc cagcttcctg gcctcattcc
    13321 tcggctggga gccactgaag gtcttgctgg aagccctgta cttctcactg gtggccaagc
    13381 ggctgcaccc ggatgaagat gacaccctgg tagagagccc ggctgtgacg cctgtgagcg
    13441 cacgtgtgcc ccgcgtacgg ccaccccacg gctttgcact cttcctggcc aaggaagaag
    13501 cccgcaaggt caagaggcta catggcatgc tgcggagcct cctggtgtac atgctttttc
    13561 tgctggtgac cctgctggcc agctatgggg atgcctcatg ccatgggcac gcctaccgtc
    13621 tgcaaagcgc catcaagcag gagctgcaca gccgggcctt cctggccatc acgcggtctg
    13681 aggagctctg gccatggatg gcccacgtgc tgctgcccta cgtccacggg aaccagtcca
    13741 gcccagagct ggggccccca cggctgcggc aggtgcggct gcaggaagca ctctacccag
    13801 accctcccgg ccccagggtc cacacgtgct cggccgcagg aggcttcagc accagcgatt
    13861 acgacgttgg ctgggagagt cctcacaatg gctcggggac gtgggcctat tcagcgccgg
    13921 atctgctggg ggcatggtcc tggggctcct gtgccgtgta tgacagcggg ggctacgtgc
    13981 aggagctggg cctgagcctg gaggagagcc gcgaccggct gcgcttcctg cagctgcaca
    14041 actggctgga caacaggagc cgcgctgtgt tcctggagct cacgcgctac agcccggccg
    14101 tggggctgca cgccgccgtc acgctgcgcc tcgagttccc ggcggccggc cgcgccctgg
    14161 ccgccctcag cgtccgcccc tttgcgctgc gccgcctcag cgcaggcctc tcgctgcctc
    14221 tgctcacctc ggtgtgcctg ctgctgttcg ccgtgcactt cgccgtggcc gaggcccgta
    14281 cttggcacag ggaagggcgc tggcgcgtgc tgcggctcgg agcctgggcg cggtggctgc
    14341 tggtggcgct gacggcggcc acggcactgg tacgcctcgc ccagctgggt gccgctgacc
    14401 gccagtggac ccgtttcgtg cgcggccgcc cgcgccgctt cactagcttc gaccaggtgg
    14461 cgcagctgag ctccgcagcc cgtggcctgg cggcctcgct gctcttcctg cttttggtca
    14521 aggctgccca gcagctacgc ttcgtgcgcc agtggtccgt ctttggcaag acattatgcc
    14581 gagctctgcc agagctcctg ggggtcacct tgggcctggt ggtgctcggg gtagcctacg
    14641 cccagctggc catcctgctc gtgtcttcct gtgtggactc cctctggagc gtggcccagg
    14701 ccctgttggt gctgtgccct gggactgggc tctctaccct gtgtcctgcc gagtcctggc
    14761 acctgtcacc cctgctgtgt gtggggctct gggcactgcg gctgtggggc gccctacggc
    14821 tgggggctgt tattctccgc tggcgctacc acgccttgcg tggagagctg taccggccgg
    14881 cctgggagcc ccaggactac gagatggtgg agttgttcct gcgcaggctg cgcctctgga
    14941 tgggcctcag caaggtcaag gagttccgcc acaaagtccg ctttgaaggg atggagccgc
    15001 tgccctctcg ctcctccagg ggctccaagg tatccccgga tgtgccccca cccagcgctg
    15061 gctccgatgc ctcgcacccc tccacctcct ccagccagct ggatgggctg agcgtgagcc
    15121 tgggccggct ggggacaagg tgtgagcctg agccctcccg cctccaagcc gtgttcgagg
    15181 ccctgctcac ccagtttgac cgactcaacc aggccacaga ggacgtctac cagctggagc
    15241 agcagctgca cagcctgcaa ggccgcagga gcagccgggc gcccgccgga tcttcccgtg
    15301 gcccatcccc gggcctgcgg ccagcactgc ccagccgcct tgcccgggcc agtcggggtg
    15361 tggacctggc cactggcccc agcaggacac cccttcgggc caagaacaag gtccacccca
    15421 gcagcactaa gcttatggtg agcaagggcg aggagctgtt caccggggtg gtgcccatcc
    15481 tggtcgagct ggacggcgac gtaaacggcc acaagttcag cgtgtccggc gagggcgagg
    15541 gcgatgccac ctacggcaag ctgaccctga agttcatctg caccaccggc aagctgcccg
    15601 tgccctggcc caccctcgtg accaccctga cctacggcgt gcagtgcttc agccgctacc
    15661 ccgaccacat gaagcagcac gacttcttca agtccgccat gcccgaaggc tacgtccagg
    15721 agcgcaccat cttcttcaag gacgacggca actacaagac ccgcgccgag gtgaagttcg
    15781 agggcgacac cctggtgaac cgcatcgagc tgaagggcat cgacttcaag gaggacggca
    15841 acatcctggg gcacaagctg gagtacaact acaacagcca caacgtctat atcatggccg
    15901 acaagcagaa gaacggcatc aaggtgaact tcaagatccg ccacaacatc gaggacggca
    15961 gcgtgcagct cgccgaccac taccagcaga acacccccat cggcgacggc cccgtgctgc
    16021 tgcccgacaa ccactacctg agcacccagt ccgccctgag caaagacccc aacgagaagc
    16081 gcgatcacat ggtcctgctg gagttcgtga ccgccgccgg gatcactctc ggcatggacg
    16141 agctgtacaa ggagaatctt tattttcagg gcaaagaaac cgctgctgct aaattcgaac
    16201 gccagcacat ggacagctga taatagattt aaatcaactt tgttgtacaa agttgcccca
    16261 ataaaatatc tttattttca ttacatctgt gtgttggttt tttgtgtggt taacaacaac
    16321 aattgcattc attttatgtt tcaggttcag ggggagatgt gggaggtttt ttaaagcaag
    16381 taaaacctct acaaatgtgg taaaatcgat aaggatcaat tgcctttttt tttaattttt
    16441 attttatttt atttttgaga tggccagatc cgggctggcg taatagcgaa gaggcccgca
    16501 ccgatcgccc ttcccaacag ttgcgcagcc tgaatggcga atggacgcgc cctgtagcgg
    16561 cgcattaagc gcggcgggtg tggtggttac gcgcagcgtg accgctacac ttgccagcgc
    16621 cctagcgccc gctcctttcg ctttcttccc ttcctttctc gccacgttcg ccggctttcc
    16681 ccgtcaagct ctaaatcggg ggctcccttt agggttccga tttagtgctt tacggcacct
    16741 cgaccccaaa aaacttgatt agggtgatgg ttcacgtagt gggccatcgc cctgatagac
    16801 ggtttttcgc cctttgacgt tggagtccac gttctttaat agtggactct tgttccaaac
    16861 tggaacaaca ctcaacccta tctcggtcta ttcttttgat ttataaggga ttttgccgat
    16921 ttcggcctat tggttaaaaa atgagctgat ttaacaaaaa tttaacgcga attttaacaa
    16981 aatattaacg cttacaattt cctgatgcgg tattttctcc ttacgcatct gtgcggtatt
    17041 tcacaccgca tatggtgcac tctcagtaca atctgctctg atgccgcata gttaagccag
    17101 ccccgacacc cgccaacacc cgctgacgcg ccctgacggg cttgtctgct cccggcatcc
    17161 gcttacagac aagctgtgac cgtctccggg agctgcatgt gtcagaggtt ttcaccgtca
    17221 tcaccgaaac gcgcgagacg aaagggcctc gtgatacgcc tatttttata ggttaatgtc
    17281 atgataataa tggtttctta gacgtcaggt ggcacttttc ggggaaatgt gcgcggaacc
    17341 cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc
    17401 tgataaatgc ttcaataata ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc
    17461 gcccttattc ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctg
    17521 gtgaaagtaa aagatgctga agatcagttg ggtgcacgag tgggttacat cgaactggat
    17581 ctcaacagcg gtaagatcct tgagagtttt cgccccgaag aacgttttcc aatgatgagc
    17641 acttttaaag ttctgctatg tggcgcggta ttatcccgta ttgacgccgg gcaagagcaa
    17701 ctcggtcgcc gcatacacta ttctcagaat gacttggttg agtactcacc agtcacagaa
    17761 aagcatctta cggatggcat gacagtaaga gaattatgca gtgctgccat aaccatgagt
    17821 gataacactg cggccaactt acttctgaca acgatcggag gaccgaagga gctaaccgct
    17881 tttttgcaca acatggggga tcatgtaact cgccttgatc gttgggaacc ggagctgaat
    17941 gaagccatac caaacgacga gcgtgacacc acgatgcctg tagcaatggc aacaacgttg
    18001 cgcaaactat taactggcga actacttact ctagcttccc ggcaacaatt aatagactgg
    18061 atggaggcgg ataaagttgc aggaccactt ctgcgctcgg cccttccggc tggctggttt
    18121 attgctgata aatctggagc cggtgagcgt gggtctcgcg gtatcattgc agcactgggg
    18181 ccagatggta agccctcccg tatcgtagtt atctacacga cggggagtca ggcaactatg
    18241 gatgaacgaa atagacagat cgctgagata ggtgcctcac tgattaagca ttggtaactg
    18301 tcagaccaag tttactcata tatactttag attgatttaa aacttcattt ttaatttaaa
    18361 aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt
    18421 tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt
    18481 tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt
    18541 ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag
    18601 ataccaaata ctgttcttct agtgtagccg tagttaggcc accacttcaa gaactctgta
    18661 gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat
    18721 aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg
    18781 ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg
    18841 agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac
    18901 aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga
    18961 aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt
    19021 ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta
    19081 cggttcctgg ccttttgctg gccttttgct cacatggctc gacagatcga tctggccaaa
    19141 aaggccggac cggatct
//
