LOCUS       sequence-208110-       11297 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentivirus production plasmid encoding for Archon2 fluorescent 
            voltage reporter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11297)
  AUTHORS   Piatkevich KD, Jung EE, Straub C, Linghu C, Park D, Suk HJ, Hochbaum
            DR, Goodwin D, Pnevmatikakis E, Pak N, Kawashima T, Yang CT, Rhoades
            JL, Shemesh O, Asano S, Yoon YG, Freifeld L, Saulnier JL, Riegler C,
            Engert F, Hughes T, Drobizhev M, Szabo B, Ahrens MB, Flavell SW, 
            Sabatini BL, Boyden ES
  TITLE     A robotic multidimensional directed evolution approach applied to 
            fluorescent voltage reporters.
  JOURNAL   Nat Chem Biol. 2018 Feb 26. pii: 10.1038/s41589-018-0004-9. doi: 
            10.1038/s41589-018-0004-9.
  PUBMED    29483642
REFERENCE   2  (bases 1 to 11297)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat Chem 
            Biol. 2018 Feb 26. pii: 10.1038/s41589-018-0004-9. doi: 
            10.1038/s41589-018-0004-9."
COMMENT     Sequence Label: pHR-EF1a-Archon2-KGC-EGFP-ER2
FEATURES             Location/Qualifiers
     source          1..11297
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             100..333
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     promoter        complement(358..376)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(359..376)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(666..685)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     complement(899..918)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     1018..1040
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(1078..1096)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        1164..1268
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(1269..1337,1338..2129)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(1487..1506)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      2300..2888
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2789..2808
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3042..3059
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(3129..3149)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        3134..3463
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      3314..3449
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     intron          4597..4662
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             4792..4812
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    5237..5371
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5274..5293)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5328..5347
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             5539..6172
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    6219..6344
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    6841..7074
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             7259..7303
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    7588..7705
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        7771..8949
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          8002..8940
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     8897..8917
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     primer_bind     8966..8985
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        8966..8984
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      9029..9038
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(9902..9904,9905..10615)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     CDS             join(9902..9904,9905..10615)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     primer_bind     complement(9944..9965)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(10205..10224)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     10552..10573
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     misc_feature    10699..11287
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(10752..10772)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(11170..11181)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(join(11290..11297,1..9))
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(join(11291..11297,1..10))
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
ORIGIN
        1 tcgacctcga gggaattaat tcgagctcgg tacctttaag accaatgact tacaaggcag
       61 ctgtagatct tagccacttt ttaaaagaaa aggggggact ggaagggcta attcactccc
      121 aacgaagaca agatctgctt tttgcttgta ctgggtctct ctggttagac cagatctgag
      181 cctgggagct ctctggctaa ctagggaacc cactgcttaa gcctcaataa agcttgcctt
      241 gagtgcttca agtagtgtgt gcccgtctgt tgtgtgactc tggtaactag agatccctca
      301 gaccctttta gtcagtgtgg aaaatctcta gcagcatcta gaattaattc cgtgtattct
      361 atagtgtcac ctaaatcgta tgtgtatgat acataaggtt atgtattaat tgtagccgcg
      421 ttctaacgac aatatgtaca agcctaattg tgtagcatct ggcttactga agcagaccct
      481 atcatctctc tcgtaaactg ccgtcagagt cggtttggtt ggacgaacct tctgagtttc
      541 tggtaacgcc gtcccgcacc cggaaatggt cagcgaacca atcagcaggg tcatcgctag
      601 ccagatcctc tacgccggac gcatcgtggc cggcatcacc ggcgccacag gtgcggttgc
      661 tggcgcctat atcgccgaca tcaccgatgg ggaagatcgg gctcgccact tcgggctcat
      721 gagcgcttgt ttcggcgtgg gtatggtggc aggccccgtg gccgggggac tgttgggcgc
      781 catctccttg catgcaccat tccttgcggc ggcggtgctc aacggcctca acctactact
      841 gggctgcttc ctaatgcagg agtcgcataa gggagagcgt cgaatggtgc actctcagta
      901 caatctgctc tgatgccgca tagttaagcc agccccgaca cccgccaaca cccgctgacg
      961 cgccctgacg ggcttgtctg ctcccggcat ccgcttacag acaagctgtg accgtctccg
     1021 ggagctgcat gtgtcagagg ttttcaccgt catcaccgaa acgcgcgaga cgaaagggcc
     1081 tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag
     1141 gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt
     1201 caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa
     1261 ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt
     1321 gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt
     1381 tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt
     1441 ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg
     1501 tattatcccg tattgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga
     1561 atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa
     1621 gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga
     1681 caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa
     1741 ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca
     1801 ccacgatgcc tgtagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta
     1861 ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac
     1921 ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc
     1981 gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag
     2041 ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga
     2101 taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt
     2161 agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata
     2221 atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag
     2281 aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa
     2341 caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt
     2401 ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgttctt ctagtgtagc
     2461 cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa
     2521 tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa
     2581 gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc
     2641 ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa
     2701 gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa
     2761 caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg
     2821 ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc
     2881 tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg
     2941 ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg
     3001 agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg
     3061 aagcggaaga gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat
     3121 gcagctgtgg aatgtgtgtc agttagggtg tggaaagtcc ccaggctccc cagcaggcag
     3181 aagtatgcaa agcatgcatc tcaattagtc agcaaccagg tgtggaaagt ccccaggctc
     3241 cccagcaggc agaagtatgc aaagcatgca tctcaattag tcagcaacca tagtcccgcc
     3301 cctaactccg cccatcccgc ccctaactcc gcccagttcc gcccattctc cgccccatgg
     3361 ctgactaatt ttttttattt atgcagaggc cgaggccgcc tcggcctctg agctattcca
     3421 gaagtagtga ggaggctttt ttggaggcct aggcttttgc aaaaagcttg gacacaagac
     3481 aggcttgcga gatatgtttg agaataccac tttatcccgc gtcagggaga ggcagtgcgt
     3541 aaaaagacgc ggactcatgt gaaatactgg tttttagtgc gccagatctc tataatctcg
     3601 cgcaacctat tttcccctcg aacacttttt aagccgtaga taaacaggct gggacacttc
     3661 acatgagcga aaaatacatc gtcacctggg acatgttgca gatccatgca cgtaaactcg
     3721 caagccgact gatgccttct gaacaatgga aaggcattat tgccgtaagc cgtggcggtc
     3781 tgtaccgggt gcgttactgg cgcgtgaact gggtattcgt catgtcgata ccgtttgtat
     3841 ttccagctac gatcacgaca accagcgcga gcttaaagtg ctgaaacgcg cagaaggcga
     3901 tggcgaaggc ttcatcgtta ttgatgacct ggtggatacc ggtggtactg cggttgcgat
     3961 tcgtgaaatg tatccaaaag cgcactttgt caccatcttc gcaaaaccgg ctggtcgtcc
     4021 gctggttgat gactatgttg ttgatatccc gcaagatacc tggattgaac agccgtggga
     4081 tatgggcgtc gtattcgtcc cgccaatctc cggtcgctaa tcttttcaac gcctggcact
     4141 gccgggcgtt gttcttttta acttcaggcg ggttacaata gtttccagta agtattctgg
     4201 aggctgcatc catgacacag gcaaacctga gcgaaaccct gttcaaaccc cgctttaaac
     4261 atcctgaaac ctcgacgcta gtccgccgct ttaatcacgg cgcacaaccg cctgtgcagt
     4321 cggcccttga tggtaaaacc atccctcact ggtatcgcat gattaaccgt ctgatgtgga
     4381 tctggcgcgg cattgaccca cgcgaaatcc tcgacgtcca ggcacgtatt gtgatgagcg
     4441 atgccgaacg taccgacgat gatttatacg atacggtgat tggctaccgt ggcggcaact
     4501 ggatttatga gtgggccccg gatctttgtg aaggaacctt acttctgtgg tgtgacataa
     4561 ttggacaaac tacctacaga gatttaaagc tctaaggtaa atataaaatt tttaagtgta
     4621 taatgtgtta aactactgat tctaattgtt tgtgtatttt agattccaac ctatggaact
     4681 gatgaatggg agcagtggtg gaatgccttt aatgaggaaa acctgttttg ctcagaagaa
     4741 atgccatcta gtgatgatga ggctactgct gactctcaac attctactcc tccaaaaaag
     4801 aagagaaagg tagaagaccc caaggacttt ccttcagaat tgctaagttt tttgagtcat
     4861 gctgtgttta gtaatagaac tcttgcttgc tttgctattt acaccacaaa ggaaaaagct
     4921 gcactgctat acaagaaaat tatggaaaaa tattctgtaa cctttataag taggcataac
     4981 agttataatc ataacatact gttttttctt actccacaca ggcatagagt gtctgctatt
     5041 aataactatg ctcaaaaatt gtgtaccttt agctttttaa tttgtaaagg ggttaataag
     5101 gaatatttga tgtatagtgc cttgactaga gatcataatc agccatacca catttgtaga
     5161 ggttttactt gctttaaaaa acctcccaca cctccccctg aacctgaaac ataaaatgaa
     5221 tgcaattgtt gttgttaact tgtttattgc agcttataat ggttacaaat aaagcaatag
     5281 catcacaaat ttcacaaata aagcattttt ttcactgcat tctagttgtg gtttgtccaa
     5341 actcatcaat gtatcttatc atgtctggat caactggata actcaagcta accaaaatca
     5401 tcccaaactt cccaccccat accctattac cactgccaat tacctgtggt ttcatttact
     5461 ctaaacctgt gattcctctg aattattttc attttaaaga aattgtattt gttaaatatg
     5521 tactacaaac ttagtagttg gaagggctaa ttcactccca aagaagacaa gatatccttg
     5581 atctgtggat ctaccacaca caaggctact tccctgatta gcagaactac acaccagggc
     5641 caggggtcag atatccactg acctttggat ggtgctacaa gctagtacca gttgagccag
     5701 ataaggtaga agaggccaat aaaggagaga acaccagctt gttacaccct gtgagcctgc
     5761 atgggatgga tgacccggag agagaagtgt tagagtggag gtttgacagc cgcctagcat
     5821 ttcatcacgt ggcccgagag ctgcatccgg agtacttcaa gaactgctga tatcgagctt
     5881 gctacaaggg actttccgct ggggactttc cagggaggcg tggcctgggc gggactgggg
     5941 agtggcgagc cctcagatcc tgcatataag cagctgcttt ttgcctgtac tgggtctctc
     6001 tggttagacc agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag
     6061 cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct
     6121 ggtaactaga gatccctcag acccttttag tcagtgtgga aaatctctag cagtggcgcc
     6181 cgaacaggga cttgaaagcg aaagggaaac cagaggagct ctctcgacgc aggactcggc
     6241 ttgctgaagc gcgcacggca agaggcgagg ggcggcgact ggtgagtacg ccaaaaattt
     6301 tgactagcgg aggctagaag gagagagatg ggtgcgagag cgtcagtatt aagcggggga
     6361 gaattagatc gcgatgggaa aaaattcggt taaggccagg gggaaagaaa aaatataaat
     6421 taaaacatat agtatgggca agcagggagc tagaacgatt cgcagttaat cctggcctgt
     6481 tagaaacatc agaaggctgt agacaaatac tgggacagct acaaccatcc cttcagacag
     6541 gatcagaaga acttagatca ttatataata cagtagcaac cctctattgt gtgcatcaaa
     6601 ggatagagat aaaagacacc aaggaagctt tagacaagat agaggaagag caaaacaaaa
     6661 gtaagaccac cgcacagcaa gcggccggcc gctgatcttc agacctggag gaggagatat
     6721 gagggacaat tggagaagtg aattatataa atataaagta gtaaaaattg aaccattagg
     6781 agtagcaccc accaaggcaa agagaagagt ggtgcagaga gaaaaaagag cagtgggaat
     6841 aggagctttg ttccttgggt tcttgggagc agcaggaagc actatgggcg cagcgtcaat
     6901 gacgctgacg gtacaggcca gacaattatt gtctggtata gtgcagcagc agaacaattt
     6961 gctgagggct attgaggcgc aacagcatct gttgcaactc acagtctggg gcatcaagca
     7021 gctccaggca agaatcctgg ctgtggaaag atacctaaag gatcaacagc tcctggggat
     7081 ttggggttgc tctggaaaac tcatttgcac cactgctgtg ccttggaatg ctagttggag
     7141 taataaatct ctggaacaga tttggaatca cacgacctgg atggagtggg acagagaaat
     7201 taacaattac acaagcttaa tacactcctt aattgaagaa tcgcaaaacc agcaagaaaa
     7261 gaatgaacaa gaattattgg aattagataa atgggcaagt ttgtggaatt ggtttaacat
     7321 aacaaattgg ctgtggtata taaaattatt cataatgata gtaggaggct tggtaggttt
     7381 aagaatagtt tttgctgtac tttctatagt gaatagagtt aggcagggat attcaccatt
     7441 atcgtttcag acccacctcc caaccccgag gggacccgac aggcccgaag gaatagaaga
     7501 agaaggtgga gagagagaca gagacagatc cattcgatta gtgaacggat ctcgacggta
     7561 tcgccaaatg gcagtattca tccacaattt taaaagaaaa ggggggattg gggggtacag
     7621 tgcaggggaa agaatagtag acataatagc aacagacata caaactaaag aattacaaaa
     7681 acaaattaca aaaattcaaa attttcgggt ttattacagg gacagcagag atccagtttg
     7741 gatcgataag cttgatatcg aattccgtga ggctccggtg cccgtcagtg ggcagagcgc
     7801 acatcgccca cagtccccga gaagttgggg ggaggggtcg gcaattgaac cggtgcctag
     7861 agaaggtggc gcggggtaaa ctgggaaagt gatgtcgtgt actggctccg cctttttccc
     7921 gagggtgggg gagaaccgta tataagtgca gtagtcgccg tgaacgttct ttttcgcaac
     7981 gggtttgccg ccagaacaca ggtaagtgcc gtgtgtggtt cccgcgggcc tggcctcttt
     8041 acgggttatg gcccttgcgt gccttgaatt acttccacct ggctgcagta cgtgattctt
     8101 gatcccgagc ttcgggttgg aagtgggtgg gagagttcga ggccttgcgc ttaaggagcc
     8161 ccttcgcctc gtgcttgagt tgaggcctgg cctgggcgct ggggccgccg cgtgcgaatc
     8221 tggtggcacc ttcgcgcctg tctcgctgct ttcgataagt ctctagccat ttaaaatttt
     8281 tgatgacctg ctgcgacgct ttttttctgg caagatagtc ttgtaaatgc gggccaagat
     8341 ctgcacactg gtatttcggt ttttggggcc gcgggcggcg acggggcccg tgcgtcccag
     8401 cgcacatgtt cggcgaggcg gggcctgcga gcgcggccac cgagaatcgg acgggggtag
     8461 tctcaagctg gccggcctgc tctggtgcct ggcctcgcgc cgccgtgtat cgccccgccc
     8521 tgggcggcaa ggctggcccg gtcggcacca gttgcgtgag cggaaagatg gccgcttccc
     8581 ggccctgctg cagggagctc aaaatggagg acgcggcgct cgggagagcg ggcgggtgag
     8641 tcacccacac aaaggaaaag ggcctttccg tcctcagccg tcgcttcatg tgactccacg
     8701 gagtaccggg cgccgtccag gcacctcgat tagttctcga gcttttggag tacgtcgtct
     8761 ttaggttggg gggaggggtt ttatgcgatg gagtttcccc acactgagtg ggtggagact
     8821 gaagttaggc cagcttggca cttgatgtaa ttctccttgg aatttgccct ttttgagttt
     8881 ggatcttggt tcattctcaa gcctcagaca gtggttcaaa gtttttttct tccatttcag
     8941 gtgtcgtgag gaattagctt ggtactaata cgactcacta tagggagacc caagctggct
     9001 aggtaagctt ggtaccgagc tcggatccgc caccatggtt tctatcgcac tgcaggctgg
     9061 ttatgacctc ctcggagatg gtagacctga aaccctgtgg ctcggaattg gtacactgct
     9121 gatgctgatc ggaaccttct acttcctggt tagagggtgg ggagtgactg acaaggatgc
     9181 acgggagtac tacgcagtgc ccatcctggt cagcggtatt gccagtgcag cttatctgtc
     9241 aatgttcttt ggtatcggcc tcacagaggt gccagtcggt ggagagatgc tggacatcta
     9301 ctacgcaaga tacgcacact ggctgttcag cacaccactg ctgctgctgg acctggcact
     9361 cctggccaaa gttgatagag tgatcatcgg cactctcgtt ggcgtcgatg ctctgatgat
     9421 tgtcacaggc ctgataggag cactgtcaca tactgccatt gccagatact cttggtggct
     9481 gttctcaact atctgtatga tcgtcgttct gtatgtcctg gccacttctc tgcggtcagc
     9541 cgctaaggag agaggcccag aggttgcctc aaccttcaac atcctcaccg cactggttct
     9601 ggtcctgtgg actgcttacc ctatcatctg gattatcggc accgagggtg ccggagttgt
     9661 gggcctggga atcgaaactc tgctgttcat ggtgctggac gtcacctgca aggtgggctt
     9721 cgggtttatc ctgctcaggt caagagccat tctgggagat acagaagcac ctgaaccttc
     9781 agctggagct gataccggtg ccgccgaccg cccggtagta gcagtgagca aggcggccgc
     9841 caagagcagg atcaccagcg agggcgagta catccccctg gaccagatcg acatcaacgt
     9901 ggtgagcaag ggcgaggagc tgttcaccgg ggtggtgccc atcctggtcg agctggacgg
     9961 cgacgtaaac ggccacaagt tcagcgtgtc cggcgagggc gagggcgatg ccacctacgg
    10021 caagctgacc ctgaagttca tttgcaccac cggcaagctg cccgtgccct ggcccaccct
    10081 cgtgaccacc ctgacctacg gcgtgcagtg cttcagccgc taccccgacc acatgaagca
    10141 gcacgacttc ttcaagtccg ccatgcccga aggctacgtc caggagcgca ccatcttctt
    10201 caaggacgac ggcaactaca agacccgcgc cgaggtgaag ttcgagggcg acaccctggt
    10261 gaaccgcatc gagctgaagg gcatcgactt caaggaggac ggcaacatcc tggggcacaa
    10321 gctggagtac aactacaaca gccacaacgt ctatatcatg gccgacaagc agaagaacgg
    10381 catcaaggtg aacttcaaga tccgccacaa catcgaggac ggcagcgtgc agctcgccga
    10441 ccactaccag cagaacaccc ccatcggcga cggccccgtg ctgctgcccg acaaccacta
    10501 cctgagcacc cagtccgccc tgagcaaaga ccccaacgag aagcgcgatc acatggtcct
    10561 gctggagttc gtgaccgccg ccgggatcac tctcggcatg gacgagctgt acaagttctg
    10621 ctacgagaac gaggtgtaag cggccgcgac tctagagtcg acctgcaggc atgcaagctt
    10681 gatatcaagc ttatcgataa tcaacctctg gattacaaaa tttgtgaaag attgactggt
    10741 attcttaact atgttgctcc ttttacgcta tgtggatacg ctgctttaat gcctttgtat
    10801 catgctattg cttcccgtat ggctttcatt ttctcctcct tgtataaatc ctggttgctg
    10861 tctctttatg aggagttgtg gcccgttgtc aggcaacgtg gcgtggtgtg cactgtgttt
    10921 gctgacgcaa cccccactgg ttggggcatt gccaccacct gtcagctcct ttccgggact
    10981 ttcgctttcc ccctccctat tgccacggcg gaactcatcg ccgcctgcct tgcccgctgc
    11041 tggacagggg ctcggctgtt gggcactgac aattccgtgg tgttgtcggg gaaatcatcg
    11101 tcctttcctt ggctgctcgc ctgtgttgcc acctggattc tgcgcgggac gtccttctgc
    11161 tacgtccctt cggccctcaa tccagcggac cttccttccc gcggcctgct gccggctctg
    11221 cggcctcttc cgcgtcttcg ccttcgccct cagacgagtc ggatctccct ttgggccgcc
    11281 tccccgcatc gataccg
//
