LOCUS       sequence-208114-        9181 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Codon-optimized Archon1 fluorescent voltage reporter in C.elegans 
            expression plasmid.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9181)
  AUTHORS   Piatkevich KD, Jung EE, Straub C, Linghu C, Park D, Suk HJ, Hochbaum
            DR, Goodwin D, Pnevmatikakis E, Pak N, Kawashima T, Yang CT, Rhoades
            JL, Shemesh O, Asano S, Yoon YG, Freifeld L, Saulnier JL, Riegler C,
            Engert F, Hughes T, Drobizhev M, Szabo B, Ahrens MB, Flavell SW, 
            Sabatini BL, Boyden ES
  TITLE     A robotic multidimensional directed evolution approach applied to 
            fluorescent voltage reporters.
  JOURNAL   Nat Chem Biol. 2018 Feb 26. pii: 10.1038/s41589-018-0004-9. doi: 
            10.1038/s41589-018-0004-9.
  PUBMED    29483642
REFERENCE   2  (bases 1 to 9181)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat Chem 
            Biol. 2018 Feb 26. pii: 10.1038/s41589-018-0004-9. doi: 
            10.1038/s41589-018-0004-9."
COMMENT     Sequence Label: pSF143-wArchon1-KGC-EGFP-ER2
FEATURES             Location/Qualifiers
     source          1..9181
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(3068..3079)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     CDS             join(5176..5178,5179..5889)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     primer_bind     complement(6732..6754)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     6854..6873
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(6955..6973)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        7041..7145
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7146..7214,7215..8006)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7364..7383)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      8177..8765
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8666..8685
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8919..8936
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    9053..9074
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(9089..9094,9095..9112,9113..9119)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9127..9143
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     9132..9154
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     9151..9167
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     9151..9167
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 gcttgcatgc gcggccgcac tgactgggcc ggcctctgtc acatactgct cgaatcggag
       61 gtaggtttga aaaacatgca attcatttcc aaaatatgac gaattttgaa aaaaaaaaat
      121 agaaaagaaa ctcaattaaa ttaccttatt ttttctagta cgtcaatttt gacttgcact
      181 taaaatctgc attttaaaaa tgaaaattca gtgcagctgc agtgcagtgc ggctattatt
      241 ttcggcattt ttgcaaaact tacacaccac cgttataaga aaatatttgt cttttttttt
      301 aaatttttat tcagtcgtta ttcgaaaaag acttacagta ccttgctgaa ttccttccgc
      361 attgcgctaa aatttaccga ttgcaaaata atcttacatg ctatagtaat aaaaaattta
      421 catacttgcc atttcatttc atttaattat ttcatccaat ttatcttgat cggtattact
      481 ctttgttaaa aattccactg ttcaattttt acatttcagt tgttttaata tcgaagtgca
      541 caagaagcgc gaacataatg gacaccatcg ctcaaaaatt atgcttctag ttgtagttcc
      601 tcgagcattt ttgatctcaa aagacatttc caagagcaat aattgccatt tttgagctac
      661 aagtaccata tcctgctcat aattcatgtt ccaccactcc ctaaagtttt ttctttggaa
      721 ttccccagac tttgcgccta aaattctcgt tattctctcc aattcaattg gctcaattca
      781 actgactaga tttcagtgat gtaggtagcg cggaactcag tattaagaga ggactcaact
      841 cagtttctaa tccaatttca acaaattcta agacaccgaa acgctgcagg tctcgtagag
      901 cctacgtgtt gacaggttct tccttttcaa tctatttccg tcgatattcc ggcgtcattt
      961 agttgaaaat gagtgaaaaa atgcgatctg acgcgaattc acgtgaactc gaaaattcga
     1021 gaatgtaatc attaagtttc cgaacaccaa gttctctttc gaaaccaagt caatagaaaa
     1081 atgagacacc cacgagaaga aggttggtcc gaaaaaagaa actaaagaag ttatttattt
     1141 ctctttttga tgtttgttga gcagaatatg atttagttaa gcacatctga tttcaacgcc
     1201 gatgaatgag gtcgtaggcg tcacgttcaa tttgatctga tttgttcagt gcttacgagt
     1261 agaaattcta aatatgatgt tactttttgg ttctgtaaga tggagctggg tatacatgtg
     1321 gtgcacggca atcgctattt ggcaatttca gtcatttcgg caaattcggc aattttggcg
     1381 aattcccata ttttggaaat tttggcaaat gcggaaaatt tcaaaaacaa gcaattacca
     1441 aatttgccaa acgccgaaaa atttaaaaaa aaaccgacat ttgctggtat tttttttaat
     1501 caggatgcta attatgtaag gttaatctat aaattcttga aacacactta actttgaaat
     1561 tgaaatcact gacatttcgc gcattcgccg ataaaatctc taaataaaat aatgtaaact
     1621 gtgtccagtt ttggtacttg tttagtatac ctggttcaaa atattttcca atgcgttttc
     1681 catttattta ttttagaaat gatcatttaa ttaaaacgta aaattcgcat tttcaagctt
     1741 agaaacatta atcctaagtc caatatggac aaaaagagta gtgtattagt accaagtaag
     1801 aaccctaaaa aaaaaaatat tctaattaaa tttttggcaa aatgccacat ttttcttttt
     1861 tttctcaacg gggaaaagtt gtgtttcttc cctttttatt aaattttttg ttttccttag
     1921 gctaagtaca acttgaaatg gaacagaaat tacataaatt tattgctttc acattttgct
     1981 cttcttcact caaatcttgg actcttgctt ttcacaaaaa aaaccttata aacaaaaata
     2041 agacatctga aaaaatgatg aacttcctgt cagtttgttc cagtatcctt cttaaatttc
     2101 cttgttttcc acatcacaaa aatcctacat cacgcttcga ctcatttcct gacagtcatc
     2161 acatcaccaa atgaatttct atttttacaa tatagttgta gtatttttgt cgaacatata
     2221 ttgttctaac taacttctta aaaaattttg ctcttgactg aacacaaatt tctgcgtcgt
     2281 ttcacgttta cttttctcaa ttttttaaaa ccaaaattta actgcaaact tctgcattgt
     2341 tctaaaaact cacaggttat ctcaaaaaga taaatttcaa agtgagattt ttttaaaaaa
     2401 attccggact gttttttggt tttgatgttt ttaatgaatt tttaaaattc tttattagtg
     2461 cctgaaatta acaataattt taaagtttgt cacactgcga ataaattaat ctcacatttc
     2521 gtatgtttag aaagatgcat gaaactccgt cgttcttatg aagttaatta agacatggct
     2581 aattgaaagc ccacatggtc atgaaaaagt aaaacttaca actttttact gaatattaac
     2641 cagtctctat aaatatccca gcttagccgg aatagggtca cgaaaacatg aaaataaagc
     2701 ttgaacctat tgttatgatt ttcaatgcca ccttttgcag tttaatatac actattacta
     2761 cttctcagtt caagttcact tctgagacgg aacctttatt ttctaacttt ctttaatctt
     2821 actttttatg agccgaatgt tctattttgt aaaatgatca gatacaagcc caaagctaat
     2881 tttgccaata agtgttagta gatcaattga attacaatat aatgacctgg gcggaataga
     2941 tttttgcact gatttttttc caagcttact ctatgaatat tcatagatca tcttcaaagg
     3001 tagcgtgtgt tgtagttgtg ttttggcggg ttcgtgttat ttgactcagc tcaagtagat
     3061 gttgtagtct tccctcaatc aacgcgtggg cttgaccctc tttaaattga gtggaagcca
     3121 gccaatctat ttgaaatcta cccccgaaat gagattcgac gatgacacga cgtgtgtgtt
     3181 acgtgcgctg agaatttagg aaaaaaaaga taatcttctt ttcgttagct gcgccacaac
     3241 ctgtgcagat aaactccgca caattgaagt tcttactggt cccacccgat ttattcaatt
     3301 gtattgtgtc tttttttctt atttctctac aactctaact ttcttcaact attctctaca
     3361 tcagtgcttt tttccaccat gaaaaaagaa atgacgtcgt tgtttcttag gaggggcgga
     3421 gcctcaactc aatggtccgt tcgaatagat cccacccctc atataaatag gactgaaacc
     3481 aaagaagttt ttaaattttc agcttaccgt gtttctcaat tttcaaaata gtcttttttg
     3541 tgtctgaacg ggctgatcaa ttatgttttt ttttgaatga aaaatcttcc tcccaagcct
     3601 taaaattcct tccaaagatc gaaagttatg tctattcaag ttacttcttc ttagatcaaa
     3661 tattaaacaa aaaaatacgc catcattctc tcacatttag taattcaaaa tttcgatttg
     3721 ttttcgaact tttgaatttc ccgcctagga agtgacatga gtgaaatctc attagataaa
     3781 tgtgccactc accgatgaca ctattttcaa ataagggggg ggggtgtttc gaataccttg
     3841 tacacgcgtt tccgcaaaac cacacacccc gagttcctca gcttcttccc ctcgacccat
     3901 gagccacgtc aagatgtcag cgtgtcactt ttcattgctt caccttctac ggtaacgaga
     3961 aaaggcgggg aatccaggat ctggtgtgac ttcttccttc ttcgtatctt cctaaaaatg
     4021 ttcgtttact ttttagcgag tgtcatttga tgggatgtcg ttcttgagga aagaggctag
     4081 aagcttaata ctctcatatt tcatattatt ggcatcaaga cgtttattat ttaactacaa
     4141 taacgattgc tttaacaact aaaaataata atttcagggt tagacaagca acggcgcgcc
     4201 tctagaggat ccccgggatt ggccaaagga cccaaaggta tgtttcgaat gatactaaca
     4261 taacatagaa cattttcagg aggacccttg gctagcgtcg acggtaccat ggtttcaatt
     4321 gctcttcaag ctggatatga tcttcttgga gatggaagac cagaatcact ttggcttgga
     4381 attggaacac ttcttatgct tattggaaca ttctatttcc ttgttagagc ttggggagaa
     4441 acagataaag atgctagaga atattatgct gttacaattc ttgtttcagg aattgcttca
     4501 gctgcttatc tttcaatgtt cttcggaatt ggacttacag aagttccagt tggaggagaa
     4561 atgcttaata tttattatgc tagatatgct caatggcttt tcacaacacc acttcttctt
     4621 cttcatcttg ctcttcttgc taaagttgat agagttacaa ttggaacact tgttggagtt
     4681 gatgctctta tgattgttac aggacttatt ggaacacttt cacatacagc tattgctaga
     4741 tattcatggt ggcttttctc aacaatttgt atgattgttg ttctttatgt tcttgctaca
     4801 tcacttagat cagctgctaa agaaagagga ccagaagttg cttcaacatt caatattctt
     4861 acagctcttg ttcttgttct ttggacagct tatccaatta tttggattat tggaacagaa
     4921 ggagctggag ttgttggact tggaattgaa acacttcttt tcatggttct tgatgttaca
     4981 gctaaagttg gattcggatt cattcttctt agatcaagag ctattcttca agatacagaa
     5041 gctccagaac catcagctgg agctgataga tctgctgctg atagaccagt tgttgctgtt
     5101 tcaaaagctg ctgctaaatc aagaattaca tcagaaggag aatatattcc acttgatcaa
     5161 attgatatta atgttgtttc aaaaggagaa gaacttttca caggagttgt tccaattctt
     5221 gttgaacttg atggagatgt taatggacat aaattctcag tttcaggaga aggagaagga
     5281 gatgctacat atggaaaact tacacttaaa ttcatttgta caacaggaaa acttccagtt
     5341 ccatggccaa cacttgttac aacacttaca tatggagttc aatgtttctc aagatatcca
     5401 gatcatatga aacaacatga tttcttcaaa tcagctatgc cagaaggata tgttcaagaa
     5461 agaacaattt tcttcaaaga tgatggaaat tataaaacaa gagctgaagt taaattcgaa
     5521 ggagatacac ttgttaatag aattgaactt aaaggaattg atttcaaaga agatggaaat
     5581 attcttggac ataaacttga atataattat aattcacata atgtttatat tatggctgat
     5641 aaacaaaaaa atggaattaa agttaatttc aaaattagac ataatattga agatggatca
     5701 gttcaacttg ctgatcatta tcaacaaaat acaccaattg gagatggacc agttcttctt
     5761 ccagataatc attatctttc aacacaatca gctctttcaa aagatccaaa tgaaaaaaga
     5821 gatcatatgg ttcttcttga attcgttaca gctgctggaa ttacacttgg aatggatgaa
     5881 ctttataaat tctgttatga aaatgaagtt tgagagctcc gcatcggccg ctgtcatcag
     5941 atcgccatct cgcgcccgtg cctctgactt ctaagtccaa ttactcttca acatccctac
     6001 atgctctttc tccctgtgct cccaccccct atttttgtta ttatcaaaaa acttctctta
     6061 atttctttgt tttttagctt cttttaagtc acctctaaca atgaaattgt gtagattcaa
     6121 aaatagaatt aattcgtaat aaaaagtcga aaaaaattgt gctccctccc cccattaata
     6181 ataattctat cccaaaatct acacaatgtt ctgtgtacac ttcttatgtt ttttacttct
     6241 gataaatttt tttgaaacat catagaaaaa accgcacaca aaatacctta tcatatgtta
     6301 cgtttcagtt tatgaccgca atttttattt cttcgcacgt ctgggcctct catgacgtca
     6361 aatcatgctc atcgtgaaaa agttttggag tatttttgga atttttcaat caagtgaaag
     6421 tttatgaaat taattttcct gcttttgctt tttggggttt cccctattgt ttgtcaagat
     6481 ttcgaggacg gcgtttttct tgctaaaatc acaagtattg atgagcacga tgcaagaaag
     6541 atcggaagaa ggtttgggtt tgaggctcag tggaaggtga gtagaagttg ataatttgaa
     6601 agtggagtag tgtctatggg gtttttgcct taaatgacag aatacattcc caatatacca
     6661 aacataactg tttctactag tcggccgtac gggccctttc gtctcgcgcg tttcggtgat
     6721 gacggtgaaa acctctgaca catgcagctc ccggagacgg tcacagcttg tctgtaagcg
     6781 gatgccggga gcagacaagc ccgtcagggc gcgtcagcgg gtgttggcgg gtgtcggggc
     6841 tggcttaact atgcggcatc agagcagatt gtactgagag tgcaccatat gcggtgtgaa
     6901 ataccgcaca gatgcgtaag gagaaaatac cgcatcaggc ggccttaatt aagggcctcg
     6961 tgatacgcct atttttatag gttaatgtca tgataataat ggtttcttag acgtcaggtg
     7021 gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa
     7081 atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga
     7141 agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg gcattttgcc
     7201 ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg
     7261 gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt gagagttttc
     7321 gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt ggcgcggtat
     7381 tatcccgtat tgacgccggg caagagcaac tcggtcgccg catacactat tctcagaatg
     7441 acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg acagtaagag
     7501 aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta cttctgacaa
     7561 cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat catgtaactc
     7621 gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag cgtgacacca
     7681 cgatgcctgt agcaatggca acaacgttgc gcaaactatt aactggcgaa ctacttactc
     7741 tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca ggaccacttc
     7801 tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc ggtgagcgtg
     7861 ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt atcgtagtta
     7921 tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc gctgagatag
     7981 gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat atactttaga
     8041 ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc
     8101 tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa
     8161 agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa
     8221 aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc
     8281 cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgttcttcta gtgtagccgt
     8341 agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc
     8401 tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac
     8461 gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca
     8521 gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg
     8581 ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag
     8641 gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt
     8701 ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat
     8761 ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc
     8821 acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt
     8881 gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag
     8941 cggaagagcg cccaatacgc aaaccgcctc tccccgcgcg ttggccgatt cattaatgca
     9001 gctggcacga caggtttccc gactggaaag cgggcagtga gcgcaacgca attaatgtga
     9061 gttagctcac tcattaggca ccccaggctt tacactttat gcttccggct cgtatgttgt
     9121 gtggaattgt gagcggataa caatttcaca caggaaacag ctatgaccat gattacgcca
     9181 a
//
