LOCUS       sequence-208119-        1054 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Monomeric near-infrared fluorescent protein miRFP in mammalian 
            expression plasmid.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1054)
  AUTHORS   Piatkevich KD, Jung EE, Straub C, Linghu C, Park D, Suk HJ, Hochbaum
            DR, Goodwin D, Pnevmatikakis E, Pak N, Kawashima T, Yang CT, Rhoades
            JL, Shemesh O, Asano S, Yoon YG, Freifeld L, Saulnier JL, Riegler C,
            Engert F, Hughes T, Drobizhev M, Szabo B, Ahrens MB, Flavell SW, 
            Sabatini BL, Boyden ES
  TITLE     A robotic multidimensional directed evolution approach applied to 
            fluorescent voltage reporters.
  JOURNAL   Nat Chem Biol. 2018 Feb 26. pii: 10.1038/s41589-018-0004-9. doi: 
            10.1038/s41589-018-0004-9.
  PUBMED    29483642
REFERENCE   2  (bases 1 to 1054)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat Chem 
            Biol. 2018 Feb 26. pii: 10.1038/s41589-018-0004-9. doi: 
            10.1038/s41589-018-0004-9."
COMMENT     Sequence Label: pmiRFP-N1
FEATURES             Location/Qualifiers
     source          1..1054
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_feature    22..102
                     /label=MCS
                     /note="multiple cloning site"
     regulatory      103..112
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 gtttagtgaa ccgtcagatc cgctagcgct accggactca gatctcgagc tcaagcttcg
       61 aattctgcag tcgacggtac cgcgggcccg ggatccaccg gtgccaccat ggtcgcagga
      121 cacgcaagtg gaagccccga ctttggaacc gccgatccga gcgactgtga gagagaagaa
      181 atccatctgg ccggatccat ccagccacac ggaacactgc tggtggtctc cgagcctgac
      241 catagaatca ttcaggcatc tgccaacgcc gctgaattcc tgaatctggg aagtgtgctg
      301 ggagtccccc tggctgagat cgacggggat ctgctgatca agattctgcc ccacctggac
      361 cctaccgcag aaggaatgcc agtggccgtc aggtgccgca ttggaaaccc ctccacagag
      421 tacgatggac tgatgcatcg accacctgaa ggaggactga tcattgagct ggaacgggct
      481 gggccaccca ttgatctgag cggaacactg gctcccgcac tggagcgaat tcgaactgca
      541 ggatccctga gggctctgtg cgacgatact gcactgctgt tccagcagtg taccggctac
      601 gaccgagtga tggtctatcg gttcgatgaa caggggcacg gagaggtgta ttcagaaata
      661 catgtgactg gactggagag ctactttggc aacaggtatc ctagctccct cgtgccacag
      721 atggcccgga gactgtacga aagacagagg gtgcgcgtcc tggtggatgt ctcttatcag
      781 cctgtgccac tggagccaag actgagtccc ctgactggca gggacctgga tatgtcaggg
      841 tgttttctgc gctctatgag tcctactcac ctgcagttcc tgaagaatat gggcgtgcgg
      901 gcaacactgg tggtcagcct ggtggtcgga ggcaaactgt gggggctggt gatctgccac
      961 cattatctgc cacgcttcat ccattttgag ctgcgagcaa tttgtgagct gctggccgaa
     1021 gctatcgcaa cccgcattac agccctgtaa gcgg
//
