LOCUS       sequence-208122-       13472 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Arabidopsis transformation, expresses HDT2 promoter/GUS fusion to 
            study expression pattern in roots.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13472)
  AUTHORS   Li H, Torres-Garcia J, Latrasse D, Benhamed M, Schilderink S, Zhou 
            W, Kulikova O, Hirt H, Bisseling T
  TITLE     Plant-Specific Histone Deacetylases HDT1/2 Regulate GIBBERELLIN 
            2-OXIDASE2 Expression to Control Arabidopsis Root Meristem Cell 
            Number.
  JOURNAL   Plant Cell. 2017 Sep;29(9):2183-2196. doi: 10.1105/tpc.17.00366. 
            Epub 2017 Aug 30.
  PUBMED    28855334
REFERENCE   2  (bases 1 to 13472)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1105/tpc.17.00366"; journalName: "Plant Cell"; date: "2017-09"; 
            volume: "29"; issue: "9"; pages: "2183-2196"
COMMENT     Sequence Label: pHDT2:GUS
FEATURES             Location/Qualifiers
     source          1..13472
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(64..1137)
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     CDS             complement(1566..2195)
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     misc_feature    3526..3550
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     primer_bind     3617..3633
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     3617..3633
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     protein_bind    3653..3673
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     protein_bind    3734..3758
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(4272..4296)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             4304..6112
                     /codon_start=1
                     /gene="uidA (or gusA) from E. coli"
                     /product="beta-glucuronidase"
                     /label=GUS
                     /translation="MLRPVETPTREIKKLDGLWAFSLDRENCGIDQRWWESALQESRAI
                     AVPGSFNDQFADADFRNYAGNVWYQREVFIPKGWAGQRIVLRFDAVTHYGKVWVNNQEV
                     MEHQGGYTPFEADVTPYVIAGKSVRITVCVNNELNWQTIPPGMVITDENGKKKQSYFHD
                     FFNYAGIHRSVMLYTTPNTWVDDITVVTHVAQDCNHASVDWQVVANGDVSVELRDADQQ
                     VVATGQGTSGTLQVVNPHLWQPGEGYLYELCVTAKSQTECDIYPLRVGIRSVAVKGEQF
                     LINHKPFYFTGFGRHEDADLRGKGFDNVLMVHDHALMDWIGANSYRTSHYPYAEEMLDW
                     ADEHGIVVIDETAAVGFNLSLGIGFEAGNKPKELYSEEAVNGETQQAHLQAIKELIARD
                     KNHPSVVMWSIANEPDTRPQGAREYFAPLAEATRKLDPTRPITCVNVMFCDAHTDTISD
                     LFDVLCLNRYYGWYVQSGDLETAEKVLEKELLAWQEKLHQPIIITEYGVDTLAGLHSMY
                     TDMWSEEYQCAWLDMYHRVFDRVSAVVGEQVWNFADFATSQGILRVGGNKKGIFTRDRK
                     PKSAAFLLQKRWTGMNFGEKPQQGGKQ"
     protein_bind    complement(6354..6374)
                     /gene="mutant version of attB"
                     /label=attB3
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     primer_bind     complement(6574..6595)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     6786..6805
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     terminator      complement(7087..7339)
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     promoter        9299..9478
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     CDS             9520..10071
                     /codon_start=1
                     /gene="Streptomyces hygroscopicus bar"
                     /product="phosphinothricin acetyltransferase"
                     /label=BlpR
                     /note="confers resistance to bialophos or phosphinothricin"
                     /translation="MSPERRPADIRRATEADMPAVCTIVNHYIETSTVNFRTEPQEPQE
                     WTDDLVRLRERYPWLVAEVDGEVAGIAYAGPWKARNAYDWTAESTVYVSPRHQRTGLGS
                     TLYTHLLKSLEAQGFKSVVAVIGLPNDPSVRMHEALGYAPRGMLRAAGFKHGNWHDVGF
                     WQLDFSLPVPPRPVLPVTEI"
     terminator      10091..10343
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     misc_feature    10436..10460
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             10981..11772
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MGEAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      12018..12606
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     12507..12526
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     12760..12777
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    12792..12932
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(12858..12877)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     13018..13040
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     rep_origin      13276..13470
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
ORIGIN
        1 aaagagacct tttcgacctt tttcccctgc tagggcaatt tgccctagca tctgctccgt
       61 acattaggaa ccggcggatg cttcgccctc gatcaggttg cggtagcgca tgactaggat
      121 cgggccagcc tgccccgcct cctccttcaa atcgtactcc ggcaggtcat ttgacccgat
      181 cagcttgcgc acggtgaaac agaacttctt gaactctccg gcgctgccac tgcgttcgta
      241 gatcgtcttg aacaaccatc tggcttctgc cttgcctgcg gcgcggcgtg ccaggcggta
      301 gagaaaacgg ccgatgccgg gatcgatcaa aaagtaatcg gggtgaaccg tcagcacgtc
      361 cgggttcttg ccttctgtga tctcgcggta catccaatca gctagctcga tctcgatgta
      421 ctccggccgc ccggtttcgc tctttacgat cttgtagcgg ctaatcaagg cttcaccctc
      481 ggataccgtc accaggcggc cgttcttggc cttcttcgta cgctgcatgg caacgtgcgt
      541 ggtgtttaac cgaatgcagg tttctaccag gtcgtctttc tgctttccgc catcggctcg
      601 ccggcagaac ttgagtacgt ccgcaacgtg tggacggaac acgcggccgg gcttgtctcc
      661 cttcccttcc cggtatcggt tcatggattc ggttagatgg gaaaccgcca tcagtaccag
      721 gtcgtaatcc cacacactgg ccatgccggc cggccctgcg gaaacctcta cgtgcccgtc
      781 tggaagctcg tagcggatca cctcgccagc tcgtcggtca cgcttcgaca gacggaaaac
      841 ggccacgtcc atgatgctgc gactatcgcg ggtgcccacg tcatagagca tcggaacgaa
      901 aaaatctggt tgctcgtcgc ccttgggcgg cttcctaatc gacggcgcac cggctgccgg
      961 cggttgccgg gattctttgc ggattcgatc agcggccgct tgccacgatt caccggggcg
     1021 tgcttctgcc tcgatgcgtt gccgctgggc ggcctgcgcg gccttcaact tctccaccag
     1081 gtcatcaccc agcgccgcgc cgatttgtac cgggccggat ggtttgcgac cgctcacgcc
     1141 gattcctcgg gcttgggggt tccagtgcca ttgcagggcc ggcagacaac ccagccgctt
     1201 acgcctggcc aaccgcccgt tcctccacac atggggcatt ccacggcgtc ggtgcctggt
     1261 tgttcttgat tttccatgcc gcctccttta gccgctaaaa ttcatctact catttattca
     1321 tttgctcatt tactctggta gctgcgcgat gtattcagat agcagctcgg taatggtctt
     1381 gccttggcgt accgcgtaca tcttcagctt ggtgtgatcc tccgccggca actgaaagtt
     1441 gacccgcttc atggctggcg tgtctgccag gctggccaac gttgcagcct tgctgctgcg
     1501 tgcgctcgga cggccggcac ttagcgtgtt tgtgcttttg ctcattttct ctttacctca
     1561 ttaactcaaa tgagttttga tttaatttca gcggccagcg cctggacctc gcgggcagcg
     1621 tcgccctcgg gttctgattc aagaacggtt gtgccggcgg cggcagtgcc tgggtagctc
     1681 acgcgctgcg tgatacggga ctcaagaatg ggcagctcgt acccggccag cgcctcggca
     1741 acctcaccgc cgatgcgcgt gcctttgatc gcccgcgaca cgacaaaggc cgcttgtagc
     1801 cttccatccg tgacctcaat gcgctgctta accagctcca ccaggtcggc ggtggcccat
     1861 atgtcgtaag ggcttggctg caccggaatc agcacgaagt cggctgcctt gatcgcggac
     1921 acagccaagt ccgccgcctg gggcgctccg tcgatcacta cgaagtcgcg ccggccgatg
     1981 gccttcacgt cgcggtcaat cgtcgggcgg tcgatgccga caacggttag cggttgatct
     2041 tcccgcacgg ccgcccaatc gcgggcactg ccctggggat cggaatcgac taacagaaca
     2101 tcggccccgg cgagttgcag ggcgcgggct agatgggttg cgatggtcgt cttgcctgac
     2161 ccgcctttct ggttaagtac agcgataacc ttcatgcgtt ccccttgcgt atttgtttat
     2221 ttactcatcg catcatatac gcagcgaccg catgacgcaa gctgttttac tcaaatacac
     2281 atcacctttt tagacggcgg cgctcggttt cttcagcggc caagctggcc ggccaggccg
     2341 ccagcttggc atcagacaaa ccggccagga tttcatgcag ccgcacggtt gagacgtgcg
     2401 cgggcggctc gaacacgtac ccggccgcga tcatctccgc ctcgatctct tcggtaatga
     2461 aaaacggttc gtcctggccg tcctggtgcg gtttcatgct tgttcctctt ggcgttcatt
     2521 ctcggcggcc gccagggcgt cggcctcggt caatgcgtcc tcacggaagg caccgcgccg
     2581 cctggcctcg gtgggcgtca cttcctcgct gcgctcaagt gcgcggtaca gggtcgagcg
     2641 atgcacgcca agcagtgcag ccgcctcttt cacggtgcgg ccttcctggt cgatcagctc
     2701 gcgggcgtgc gcgatctgtg ccggggtgag ggtagggcgg gggccaaact tcacgcctcg
     2761 ggccttggcg gcctcgcgcc cgctccgggt gcggtcgatg attagggaac gctcgaactc
     2821 ggcaatgccg gcgaacacgg tcaacaccat gcggccggcc ggcgtggtgg tgtcggccca
     2881 cggctctgcc aggctacgca ggcccgcgcc ggcctcctgg atgcgctcgg caatgtccag
     2941 taggtcgcgg gtgctgcggg ccaggcggtc tagcctggtc actgtcacaa cgtcgccagg
     3001 gcgtaggtgg tcaagcatcc tggccagctc cgggcggtcg cgcctggtgc cggtgatctt
     3061 ctcggaaaac agcttggtgc agccggccgc gtgcagttcg gcccgttggt tggtcaagtc
     3121 ctggtcgtcg gtgctgacgc gggcatagcc cagcaggcca gcggcggcgc tcttgttcat
     3181 ggcgtaatgt ctccggttct agtcgcaagt attctacttt atgcgactaa aacacgcgac
     3241 aagaaaacgc caggaaaagg gcagggcggc agcctgtcgc gtaacttagg acttgtgcga
     3301 catgtcgttt tcagaagacg gctgcactga acgtcagaag ccgactgcac tatagcagcg
     3361 gaggggttgg atcaaagtac tttaaagtac tttaaagtac tttaaagtac tttgatcccg
     3421 aggggaaccc tgtggttggc atgcacatac aaatggacga acggataaac cttttcacgc
     3481 ccttttaaat atccgattat tctaataaac gctcttttct cttaggttta cccgccaata
     3541 tatcctgtca aacactgata gtttaaactg aaggcgggaa acgacaatct gatccaagct
     3601 caagctaagc ttcacacagg aaacagctat gaccatgatt acgccaagct atcaactttg
     3661 tatagaaaag ttggccagag accaagcttc tcgagggtct ctcgcgccaa agggcgcgcc
     3721 taacccgggt accacaagtt tgtacaaaaa agcaggctcc gcggccgccc ccttcaccgc
     3781 tctcttcttc tgtagcattt agctcgagtt cttatgcact tcaaggcttc tttttttggt
     3841 ggacaagatc atttgaattt ttgttgtatt atctaataaa tattttaccg aaactattta
     3901 aacgaaaaaa ctgaagattt atttctgttt tatttttcat aatcatatga tttagagaat
     3961 gagaatcgaa taatctcaga atatttaagt taatatttca tttatcaatt tagaatcttt
     4021 accaaatatg caaataattt tacattaacg caaatattcc aaaataaagg aagcgttacg
     4081 aaattaaggg caaaacgacg ccgtgtagac tctcgaagaa ccctaaagag acttcttaaa
     4141 cctcattcga ttataaaata gccccaaacc cactgcctct ttcactgcaa tttctctctt
     4201 tcgcttctaa aaaaaaacct aacaacctct cttctctctt cctcgttcaa caacaaaggg
     4261 tgggcgcgcc gacccagctt tcttgtacaa agtggggcgc gccatgttac gtcctgtaga
     4321 aaccccaacc cgtgaaatca aaaaactcga cggcctgtgg gcattcagtc tggatcgcga
     4381 aaactgtgga attgatcagc gttggtggga aagcgcgtta caagaaagcc gggcaattgc
     4441 tgtgccaggc agttttaacg atcagttcgc cgatgcagat tttcgtaatt atgcgggcaa
     4501 cgtctggtat cagcgcgaag tctttatacc gaaaggttgg gcaggccagc gtatcgtgct
     4561 gcgtttcgat gcggtcactc attacggcaa agtgtgggtc aataatcagg aagtgatgga
     4621 gcatcagggc ggctatacgc catttgaagc cgatgtcacg ccgtatgtta ttgccgggaa
     4681 aagtgtacgt atcaccgttt gcgtgaacaa cgaactgaac tggcagacta tcccgccggg
     4741 aatggtgatt accgacgaaa acggcaagaa aaagcagtct tacttccatg atttctttaa
     4801 ctatgccgga atccatcgca gcgtaatgct ctacaccacg ccgaacacct gggtggacga
     4861 tatcaccgtg gtgacgcatg tcgcgcaaga ctgtaaccac gcgtctgttg actggcaggt
     4921 ggtggccaat ggtgatgtca gcgttgaact gcgtgatgcg gatcaacagg tggttgcaac
     4981 tggacaaggc actagcggga ctttgcaagt ggtgaatccg cacctctggc aaccgggtga
     5041 aggttatctc tatgaactgt gcgtcacagc caaaagccag acagagtgtg atatctaccc
     5101 gcttcgcgtc ggcatccggt cagtggcagt gaagggcgaa cagttcctga ttaaccacaa
     5161 accgttctac tttactggct ttggtcgtca tgaagatgcg gacttgcgtg gcaaaggatt
     5221 cgataacgtg ctgatggtgc acgaccacgc attaatggac tggattgggg ccaactccta
     5281 ccgtacctcg cattaccctt acgctgaaga gatgctcgac tgggcagatg aacatggcat
     5341 cgtggtgatt gatgaaactg ctgctgtcgg ctttaacctc tctttaggca ttggtttcga
     5401 agcgggcaac aagccgaaag aactgtacag cgaagaggca gtcaacgggg aaactcagca
     5461 agcgcactta caggcgatta aagagctgat agcgcgtgac aaaaaccacc caagcgtggt
     5521 gatgtggagt attgccaacg aaccggatac ccgtccgcaa ggtgcacggg aatatttcgc
     5581 gccactggcg gaagcaacgc gtaaactcga cccgacgcgt ccgatcacct gcgtcaatgt
     5641 aatgttctgc gacgctcaca ccgataccat cagcgatctc tttgatgtgc tgtgcctgaa
     5701 ccgttattac ggatggtatg tccaaagcgg cgatttggaa acggcagaga aggtactgga
     5761 aaaagaactt ctggcctggc aggagaaact gcatcagccg attatcatca ccgaatacgg
     5821 cgtggatacg ttagccgggc tgcactcaat gtacaccgac atgtggagtg aagagtatca
     5881 gtgtgcatgg ctggatatgt atcaccgcgt ctttgatcgc gtcagcgccg tcgtcggtga
     5941 acaggtatgg aatttcgccg attttgcgac ctcgcaaggc atattgcgcg ttggcggtaa
     6001 caagaaaggg atcttcactc gcgaccgcaa accgaagtcg gcggcttttc tgctgcaaaa
     6061 acgctggact ggcatgaact tcggtgaaaa accgcagcag ggaggcaaac aaggtaccta
     6121 accgcggcca tgctagagtc cgcaaaaatc accagtctct ctctacaaat ctatctctct
     6181 ctatttttct ccagaataat gtgtgagtag ttcccagata agggaattag ggttcttata
     6241 gggtttcgct catgtgttga gcatataaga aacccttagt atgtatttgt atttgtaaaa
     6301 tacttctatc aataaaattt ctaattccta aaaccaaaat ccagtgatcc ggacaacttt
     6361 attatacata gttgataatt cactggccgt cgtgacgtcg ggcccatcga tggcgcgctg
     6421 attgcccttc accgcctggc cctgagagag ttgcagcaag cggtccacgc tggtttgccc
     6481 cagcaggcga aaatcctgtt tgatggtggt tccgaaatcg gcaaaatccc ttataaatca
     6541 aaagaatagc ccgagatagg gttgagtgtt gttccagttt ggaacaagag tccactatta
     6601 aagaacgtgg actccaacgt caaagggcga aaaaccgtct atcagggcga tggcccacta
     6661 cgtgaaccat cacccaaatc aagttttttg gggtcgaggt gccgtaaagc actaaatcgg
     6721 aaccctaaag ggagcccccg atttagagct tgacggggaa agccggcgaa cgtggcgaga
     6781 aaggaaggga agaaagcgaa aggagcgggc gccattcagg ccgatagcag caccgtaatc
     6841 agtagcgaca gaatcaagtt tgcctttagc gtcagactgt agcgcgtttt catcggcatt
     6901 ttcggtcata gcccccttat tagcgtttgc catcttttca taatcaaaat caccggaacc
     6961 agagccacca ccggaaccgc ctccctcaga gccgccaccc tcagaaccgc caccctcaga
     7021 gccaccaccc tcagagccgc caccagaacc accaccagag ccgccgccag cattgacagg
     7081 aggcccgatc tagtaacata gatgacaccg cgcgcgataa tttatcctag tttgcgcgct
     7141 atattttgtt ttctatcgcg tattaaatgt ataattgcgg gactctaatc ataaaaaccc
     7201 atctcataaa taacgtcatg cattacatgt taattattac atgcttaacg taattcaaca
     7261 gaaattatat gataatcatc gcaagaccgg caacaggatt caatcttaag aaactttatt
     7321 gccaaatgtt tgaacgatcg gggatccgct ttacttgtac ataaggaaca gatggtggcg
     7381 tccctcggtt ctttcatact gctcaacgat tgtatagtct tcgttgtggc ttgttatatc
     7441 cagtttggag tcaacatagt agtaccctgg tagctgcaca ggcttctttg ccatgtaaat
     7501 acttttgaat tcaactaggt aatgaccacc gtctttcagc ttcagagcct tatgaatctc
     7561 tcctttcaac acgccatcac gaggatacaa acgctcagtg ctggcttccc agcccattgt
     7621 cttcttttgc ataacaggtc catcggaagg aaagttcacg ccaatgaact tgaccttgta
     7681 gatgaaacag ccatcctgca aactggaatc ctgggttaca gtaacgacgc caccgtcttc
     7741 aaagttcatg accctttccc atttaaatcc ttcaggaaat gacagctttt tatagtctgg
     7801 tatgtcggca gggtgcttga catatacctt gcttccatac tgaaattgtg gtgacaaaat
     7861 atcccaagca aatggcaaag gtcccccctt ggttacctta agctttacgg tattgtggcc
     7921 ttcgtatggc ctcccctctc cttcgccttc tatttcaaac tcgtgcccat tgaccgttcc
     7981 ttccatgcga accttaaacc tcatgaactc cttgataaca ttcttggaag accccatggg
     8041 gtatgttttt aatcttgttt gtattgatga gttttggttt gagtaaagag tgaagcggat
     8101 gagttaattt atagactata aaggagattt gcatggcgat cacgtgtaat aatgcatgca
     8161 cgcatgtgat tgtatgtgtg tgctgtgaga gagaagctct taggtgtttg aagggagtga
     8221 caagtggcga agaaaaacaa ttctccgcgg ctgcatgcta tgtgtaacgt gtagctaatg
     8281 ttctggcatg gcatcttatg aacgattctt tttaaaaaca aggtaaaaac ttaacttcat
     8341 aaaattaaaa aaaagagcgt ttactaagtt ggtttaaaag gggatgagag tctataaatt
     8401 ttggaggtag tgccgttggg aatataaatt gggagcttaa tcagaattat agaagttaaa
     8461 gttgatttag tcacggtcaa tataaattgg gaatttgagt caaaatcttc caaattcgga
     8521 atccgtcttg ttacacccgg tggataggag ccgaacggtt tgaaaatact tgaaatgtgg
     8581 atgcaggtgc aggctggttt aattttatgt tgaatggata catgtcaatc gaatttgagt
     8641 tataggtaca cattttactc tgatactaaa atgtaacatt tgtctcaaga atgggtaggt
     8701 catccttact cgacaacggt atgccacttc acttgaacgt atatctcatt caacccaact
     8761 ctaacacaca tttcataccc atgtctaaaa taaagaaaat tgaagaaaaa cgctcaatag
     8821 cgtcaatcct tacaaatata atgtacatgg caacctcgca tgaaaatagt aataaagagg
     8881 agtgaataat ataaagtagt cactaattgg atgacaaagc aaatggaacc atccgataga
     8941 tgttttgaga acaagaggta catttgtata tatgcattgg ttgatacatt tctgccatct
     9001 gaattgtttc ttcctcaaag aagaagcatt cagccaatag tgaaatttaa aatgttggaa
     9061 aaatgaggat gcagtcagaa agtccagaat ataagtggta actggtaact gaatacttgc
     9121 ctcagaagca agataagaaa cataagtctt taaaggaatc aatcaccgat gaagagagct
     9181 ccgaatcgaa ttcgagctcg gtaccgcgaa ttatcgatca tgagcggaga attaagggag
     9241 tcacgttatg acccccgccg atgacgcggg acaagccgtt ttacgtttgg aactgacaga
     9301 accgcaacgt tgaaggagcc actgagccgc gggtttctgg agtttaatga gctaagcaca
     9361 tacgtcagaa accattattg cgcgttcaaa agtcgcctaa ggtcactatc agctagcaaa
     9421 tatttcttgt caaaaatgct ccactgacgt tccataaatt cccctcggta tccaattaga
     9481 gtctcatatt cactctcaac tcgatcgagg ggatctacca tgagcccaga acgacgcccg
     9541 gccgacatcc gccgtgccac cgaggcggac atgccggcgg tctgcaccat cgtcaaccac
     9601 tacatcgaga caagcacggt caacttccgt accgagccgc aggaaccgca ggagtggacg
     9661 gacgacctcg tccgtctgcg ggagcgctat ccctggctcg tcgccgaggt ggacggcgag
     9721 gtcgccggca tcgcctacgc gggtccctgg aaggcacgca acgcctacga ctggacggcc
     9781 gagtcgaccg tgtacgtctc cccccgccac cagcggacgg gactgggctc cacgctctac
     9841 acccacctgc tgaagtccct ggaggcacag ggcttcaaga gcgtggtcgc tgtcatcggg
     9901 ctgcccaacg acccgagcgt gcgcatgcac gaggcgctcg gatatgcccc ccgcggcatg
     9961 ctgcgggcgg ccggcttcaa gcacgggaac tggcatgacg tgggtttctg gcagctggac
    10021 ttcagcctgc cggtgccgcc ccgtccggtc ctgcccgtca ccgaaatctg atgaccccta
    10081 gagtcaagca gatcgttcaa acatttggca ataaagtttc ttaagattga atcctgttgc
    10141 cggtcttgcg atgattatca tataatttct gttgaattac gttaagcatg taataattaa
    10201 catgtaatgc atgacgttat ttatgagatg ggtttttatg attagagtcc cgcaattata
    10261 catttaatac gcgatagaaa acaaaatata gcgcgcaaac taggataaat tatcgcgcgc
    10321 ggtgtcatct atgttactag atcgaccggc atgcaagctg ataattcaat tcggcgttaa
    10381 ttcagtacat taaaaacgtc cgcaatgtgt tattaagttg tctaagcgtc aatttgttta
    10441 caccacaata tatcctgcca ccagccagcc aacagctccc cgaccggcag ctcggcacaa
    10501 aatcaccact cgatacaggc agcccatcag tccgggacgg cgtcagcggg agagccgttg
    10561 taaggcggca gactttgctc atgttaccga tgctattcgg aagaacggca actaagctgc
    10621 cgggtttgaa acacggatga tctcgcggag ggtagcatgt tgattgtaac gatgacagag
    10681 cgttgctgcc tgtgatcaat tcgggcacga acccagtgga cataagcctg ttcggttcgt
    10741 aagctgtaat gcaagtagcg tatgcgctca cgcaactggt ccagaacctt gaccgaacgc
    10801 agcggtggta acggcgcagt ggcggttttc atggcttgtt atgactgttt ttttggggta
    10861 cagtctatgc ctcgggcatc caagcagcaa gcgcgttacg ccgtgggtcg atgtttgatg
    10921 ttatggagca gcaacgatgt tacgcagcag ggcagtcgcc ctaaaacaaa gttaaacatc
    10981 atgggggaag cggtgatcgc cgaagtatcg actcaactat cagaggtagt tggcgtcatc
    11041 gagcgccatc tcgaaccgac gttgctggcc gtacatttgt acggctccgc agtggatggc
    11101 ggcctgaagc cacacagtga tattgatttg ctggttacgg tgaccgtaag gcttgatgaa
    11161 acaacgcggc gagctttgat caacgacctt ttggaaactt cggcttcccc tggagagagc
    11221 gagattctcc gcgctgtaga agtcaccatt gttgtgcacg acgacatcat tccgtggcgt
    11281 tatccagcta agcgcgaact gcaatttgga gaatggcagc gcaatgacat tcttgcaggt
    11341 atcttcgagc cagccacgat cgacattgat ctggctatct tgctgacaaa agcaagagaa
    11401 catagcgttg ccttggtagg tccagcggcg gaggaactct ttgatccggt tcctgaacag
    11461 gatctatttg aggcgctaaa tgaaacctta acgctatgga actcgccgcc cgactgggct
    11521 ggcgatgagc gaaatgtagt gcttacgttg tcccgcattt ggtacagcgc agtaaccggc
    11581 aaaatcgcgc cgaaggatgt cgctgccgac tgggcaatgg agcgcctgcc ggcccagtat
    11641 cagcccgtca tacttgaagc tagacaggct tatcttggac aagaagaaga tcgcttggcc
    11701 tcgcgcgcag atcagttgga agaatttgtc cactacgtga aaggcgagat caccaaggta
    11761 gtcggcaaat aatgtctagc tagaaattcg ttcaagccga cgccgcttcg cggcgcggct
    11821 taactcaagc gttagatgca ctaagcacat aattgctcac agccaaacta tcaggtcaag
    11881 tctgctttta ttatttttaa gcgtgcataa taagccctac acaaattggg agatatatca
    11941 tgcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa
    12001 aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca
    12061 aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt
    12121 ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgtccttct agtgtagccg
    12181 tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc
    12241 ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga
    12301 cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc
    12361 agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc
    12421 gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca
    12481 ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg
    12541 tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta
    12601 tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct
    12661 cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag
    12721 tgagctgata ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa
    12781 gcggaagagc gcctgatgcg gtattttctc cttacgcatc tgtgcggtat ttcacaccgc
    12841 atatggtgca ctctcagtac aatctgctct gatgccgcat agttaagcca gtatacactc
    12901 cgctatcgct acgtgactgg gtcatggctg cgccccgaca cccgccaaca cccgctgacg
    12961 cgccctgacg ggcttgtctg ctcccggcat ccgcttacag acaagctgtg accgtctccg
    13021 ggagctgcat gtgtcagagg ttttcaccgt catcaccgaa acgcgcgagg cagggtgcct
    13081 tgatgtgggc gccggcggtc gagtggcgac ggcgcggctt gtccgcgccc tggtagattg
    13141 cctggccgta ggccagccat ttttgagcgg ccagcggccg cgataggccg acgcgaagcg
    13201 gcggggcgta gggagcgcag cgaccgaagg gtaggcgctt tttgcagctc ttcggctgtg
    13261 cgctggccag acagttatgc acaggccagg cgggttttaa gagttttaat aagttttaaa
    13321 gagttttagg cggaaaaatc gccttttttc tcttttatat cagtcactta catgtgtgac
    13381 cggttcccaa tgtacggctt tgggttccca atgtacgggt tccggttccc aatgtacggc
    13441 tttgggttcc caatgtacgt gctatccaca gg
//
