LOCUS       sequence-208131-         764 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Plasmid expressing two multiplexed ribozyme-gRNA-ribozyme (RGR) 
            gRNAs.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 764)
  AUTHORS   Marshall JM, Buchman A, Sanchez C HM, Akbari OS
  TITLE     Overcoming evolved resistance to population-suppressing homing-based
            gene drives.
  JOURNAL   Sci Rep. 2017 Jun 19;7(1):3776. doi: 10.1038/s41598-017-02744-7.
  PUBMED    28630470
REFERENCE   2  (bases 1 to 764)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Rep."; 
            date: "2017-06-19"; volume: "7(1):3776. doi"; pages: " 
            10.1038/s41598-017-02744-7"
COMMENT     Sequence Label: OA-16
FEATURES             Location/Qualifiers
     source          1..764
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_RNA        130..205
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     ncRNA           210..277
                     /product="hepatitis delta virus self-cleaving ribozyme 
                     (Ferre-D'Amare and Scott, 2010)"
                     /label=HDV ribozyme
                     /ncRNA_class="ribozyme"
     misc_RNA        343..418
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     ncRNA           423..490
                     /product="hepatitis delta virus self-cleaving ribozyme 
                     (Ferre-D'Amare and Scott, 2010)"
                     /label=HDV ribozyme
                     /ncRNA_class="ribozyme"
     intron          568..633
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
ORIGIN
        1 caacgaaact catttgattt cgtactaatt ttccacatct ctattttgac ccgcagaggc
       61 gcgccatcgc cctgatgagt cccgtgagga cgaaacgagt aagctcgtcg gcgatacttg
      121 gatgccctgg ttttagagct agaaatagca agttaaaata aggctagtcc gttatcaact
      181 tgaaaaagtg gcaccgagtc ggtgcttttg gccggcatgg tcccagcctc ctcgctggcg
      241 ccggctgggc aacatgcttc ggcatggcga atgggacaaa aaccctgatg agtcccgtga
      301 ggacgaaacg agtaagctcg tcggttttgg acactggaac cggttttaga gctagaaata
      361 gcaagttaaa ataaggctag tccgttatca acttgaaaaa gtggcaccga gtcggtgctt
      421 ttggccggca tggtcccagc ctcctcgctg gcgccggctg ggcaacatgc ttcggcatgg
      481 cgaatgggac agatctttgt gaaggaacct tacttctgtg gtgtgacata attggacaaa
      541 ctacctacag agatttaaag ctctaaggta aatataaaat ttttaagtgt ataatgtgtt
      601 aaactactga ttctaattgt ttgtgtattt tagattccaa cctatggaac tgatgaatgg
      661 gagcagtggt ggaatgcctt taatgaggaa aacctgtttt gctcagaaga aatgccatct
      721 agtgatgatg aggctactgc tgactctcaa cattctactc ctcc
//
