LOCUS       sequence-208151-       10989 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral expression plasmid of human SIK3 cDNA (gatekeeper 
            mutation) with neomycin resistance gene.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10989)
  AUTHORS   Tarumoto Y, Lu B, Somerville TDD, Huang YH, Milazzo JP, Wu XS, 
            Klingbeil O, El Demerdash O, Shi J, Vakoc CR
  TITLE     LKB1, Salt-Inducible Kinases, and MEF2C Are Linked Dependencies in 
            Acute Myeloid Leukemia.
  JOURNAL   Mol Cell. 2018 Feb 28. pii: S1097-2765(18)30109-6. doi: 
            10.1016/j.molcel.2018.02.011.
  PUBMED    29526696
REFERENCE   2  (bases 1 to 10989)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell. 
            2018 Feb 28. pii: S1097-2765(18)30109-6. doi: 
            10.1016/j.molcel.2018.02.011."
COMMENT     Sequence Label: LentiV_Neo_SIK3_T142Q
FEATURES             Location/Qualifiers
     source          1..10989
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        13..239
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             240..420
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    467..592
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1085..1318
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1503..1547
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    1731..1848
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        1917..2128
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     CDS             5969..6025
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             6044..6838
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(6098..6117)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     6708..6727
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     misc_feature    6854..7442
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(6907..6927)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(7325..7336)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             7514..7747
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    7825..7946
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7862..7881)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7916..7935
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      7986..8121
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(8141..8160)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(8142..8160)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(8170..8187)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(8170..8186)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8179..8201)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      8328..8783
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8415..8434)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     8625..8646
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        8809..8913
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(8914..8982,8983..9774)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(9132..9151)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      9945..10533
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     10434..10453
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     10687..10704
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    10821..10842
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(10857..10862,10863..10880,10881..10887)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    10895..10911
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     10900..10922
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     10919..10935
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     10919..10935
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     10954..10974
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        10956..10974
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
        1 ctgcaagctt aatgtagtct tatgcaatac tcttgtagtc ttgcaacatg gtaacgatga
       61 gttagcaaca tgccttacaa ggagagaaaa agcaccgtgc atgccgattg gtggaagtaa
      121 ggtggtacga tcgtgcctta ttaggaaggc aacagacggg tctgacatgg attggacgaa
      181 ccactgaatt gccgcattgc agagatattg tatttaagtg cctagctcga tacataaacg
      241 ggtctctctg gttagaccag atctgagcct gggagctctc tggctaacta gggaacccac
      301 tgcttaagcc tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt
      361 gtgactctgg taactagaga tccctcagac ccttttagtc agtgtggaaa atctctagca
      421 gtggcgcccg aacagggact tgaaagcgaa agggaaacca gaggagctct ctcgacgcag
      481 gactcggctt gctgaaacgc gcacggcaag aggcgagggg cggcgactgg tgagtacgcc
      541 aaaaattttg actagcggag gctagaagga gagagatggg tgcgagagcg tcagtattaa
      601 gcgggggaga attagatcgc gatgggaaaa aattcggtta aggccagggg gaaagaaaaa
      661 atataaatta aaacatatag tatgggcaag cagggagcta gaacgattcg cagttaatcc
      721 tggcctgtta gaaacatcag aaggctgtag acaaatactg ggacagctac aaccatccct
      781 tcagacagga tcagaagaac ttagatcatt atataataca gtagcaaccc tctattgtgt
      841 gcatcaaagg atagagataa aagacaccaa ggaagcttta gacaagatag aggaagagca
      901 aaacaaaagt aagaccaccg cacagcaagc ggccgctgat cttcagacct ggaggaggag
      961 atatgaggga caattggaga agtgaattat ataaatataa agtagtaaaa attgaaccat
     1021 taggagtagc acccaccaag gcaaagagaa gagtggtgca gagagaaaaa agagcagtgg
     1081 gaataggagc tttgttcctt gggttcttgg gagcagcagg aagcactatg ggcgcagcgt
     1141 caatgacgct gacggtacag gccagacaat tattgtctgg tatagtgcag cagcagaaca
     1201 atttgctgag ggctattgag gcgcaacagc atctgttgca actcacagtc tggggcatca
     1261 agcagctcca ggcaagaatc ctggctgtgg aaagatacct aaaggatcaa cagctcctgg
     1321 ggatttgggg ttgctctgga aaactcattt gcaccactgc tgtgccttgg aatgctagtt
     1381 ggagtaataa atctctggaa cagatttgga atcacacgac ctggatggag tgggacagag
     1441 aaattaacaa ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag
     1501 aaaagaatga acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta
     1561 acataacaaa ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag
     1621 gtttaagaat agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac
     1681 cattatcgtt tcagacccac ctcccaaccc cgaggggacc cagaccacaa ttttaaaaga
     1741 aaagggggga ttggggggta cagtgcaggg gaaagaatag tagacataat agcaacagac
     1801 atacaaacta aagaattaca aaaacaaatt acaaaaattc aaaattttcg ggtttattac
     1861 agggacagca gagatccact ttggcgccgg ctcgagtggc tccggtgccc gtcagtgggc
     1921 agagcgcaca tcgcccacag tccccgagaa gttgggggga ggggtcggca attgaaccgg
     1981 tgcctagaga aggtggcgcg gggtaaactg ggaaagtgat gtcgtgtact ggctccgcct
     2041 ttttcccgag ggtgggggag aaccgtatat aagtgcagta gtcgccgtga acgttctttt
     2101 tcgcaacggg tttgccgcca gaacacaggt gtcgtgacgc gggatccgcc acccggaggt
     2161 ttcactgcac aacaagatgg cggcggcggc ggcgagcgga gctggcgggg ctgccggggc
     2221 cgggactggg ggagccgggc ccgcgggccg cctgctgcct ccgcccgcgc cggggtcccc
     2281 agccgccccc gctgccgtgt cccctgcggc cggccagccg cgtcccccag ccccggcctc
     2341 ccgcggaccc atgcccgccc gtatcggcta ctacgagatc gaccgcacca tcggcaaggg
     2401 caacttcgcg gtggtcaagc gggccacgca cctcgtcacc aaggccaagg ttgctatcaa
     2461 gatcatagat aagacccagc tggatgaaga aaacttgaag aagattttcc gggaagttca
     2521 aattatgaag atgctttgcc acccccatat catcaggctc taccaggtta tggagacaga
     2581 acggatgatt tatctggtgc aagaatatgc tagtggaggg gaaatatttg accacctggt
     2641 ggcccatggt agaatggcag aaaaggaggc acgtcggaag ttcaaacaga tcgtcacagc
     2701 tgtctatttt tgtcactgtc ggaacattgt tcatcgtgat ttaaaagctg aaaatttact
     2761 tctggatgcc aatctgaata tcaaaatagc agattttggt ttcagtaacc tcttcactcc
     2821 tgggcagctg ctgaagacct ggtgtggcag ccctccctat gctgcacctg aactctttga
     2881 aggaaaagaa tatgatgggc ccaaagtgga catctggagc cttggagttg tcctctacgt
     2941 gcttgtgtgc ggtgccctgc catttgatgg aagcacactg cagaatctgc gggcccgcgt
     3001 gctgagtgga aagttccgca tcccattttt tatgtccaca gaatgtgagc atttgatccg
     3061 ccatatgttg gtgttagatc ccaataagcg cctctccatg gagcagatct gcaagcacaa
     3121 gtggatgaag ctaggggacg ccgatcccaa ctttgacagg ttaatagctg aatgccaaca
     3181 actaaaggaa gaaagacagg tggaccccct gaatgaggat gtcctcttgg ccatggagga
     3241 catgggactg gacaaagaac agacactgca ggcggagcag gcaggtactg ctatgaacat
     3301 cagcgttccc caggtgcagc tgatcaaccc agagaaccaa attgtggagc cggatgggac
     3361 actgaatttg gacagtgatg agggtgaaga gccttcccct gaagcattgg tgcgctattt
     3421 gtcaatgagg aggcacacag tgggtgtggc tgacccacgc acggaagtta tggaagatct
     3481 gcagaagctc ctacctggct ttcctggagt caacccccag gctccattcc tgcaggtggc
     3541 ccctaatgtg aacttcatgc acaacctgtt gcctatgcaa aacttgcaac caaccgggca
     3601 acttgagtac aaggagcagt ctctcctaca gccgcccacg ctacagctgt tgaatggaat
     3661 gggccccctt ggccggaggg catcagatgg aggagccaac atccaactgc atgcccagca
     3721 gctgctgaag cgcccacggg gaccctctcc gcttgtcacc atgacaccag cagtgccagc
     3781 agttacccct gtggacgagg agagctcaga cggggagcca gaccaggaag ctgtgcagag
     3841 gtacttggca aataggtcca aaagacatac actggccatg accaacccta cagctgagat
     3901 cccaccggac ctacaacggc agctaggaca gcagcctttc cgttcccggg tctggcctcc
     3961 tcacctggta cctgatcagc atcgctctac ctacaaggac tccaacactc tgcacctccc
     4021 tacggagcgt ttctcccctg tgcgccggtt ctcagatggg gctgcgagca tccaggcctt
     4081 caaagctcac ctggaaaaaa tgggcaacaa cagcagcatc aaacagctgc agcaggagtg
     4141 tgagcagctg cagaagatgt acggggggca gattgatgaa agaaccctgg agaagaccca
     4201 gcagcagcat atgttatacc agcaggagca gcaccatcaa attctccagc aacaaattca
     4261 agactctatc tgtcctcctc agccatctcc acctcttcag gctgcatgtg aaaatcagcc
     4321 agccctcctt acccatcagc tccagaggtt aaggattcag ccttcaagcc caccccccaa
     4381 ccaccccaac aaccatctct tcaggcagcc cagtaatagt cctcccccca tgagcagtgc
     4441 catgatccag cctcacgggg ctgcatcttc ttcccagttt caaggcttac cttcccgcag
     4501 tgcaatcttt cagcagcaac ctgagaactg ttcctctcct cccaacgtgg cactaacctg
     4561 cttgggtatg cagcagcctg ctcagtcaca gcaggtcacc atccaagtcc aagagcctgt
     4621 tgacatgctc agcaacatgc caggcacagc tgcaggctcc agtgggcgcg gcatctccat
     4681 cagccccagt gctggtcaga tgcagatgca gcaccgtacc aacctgatgg ccaccctcag
     4741 ctatgggcac cgtcccttgt ccaagcagct gagtgctgac agtgcagagg ctcacagtgc
     4801 acatcagcag ccgccacact ataccacgtc ggcactacag caggccctgc tgtctcccac
     4861 gccgccagac tatacaagac accagcaggt accccacatc cttcaaggac tgctttctcc
     4921 ccggcattcg ctcaccggcc actcggacat ccggctgccc ccaacagagt ttgcacagct
     4981 cattaaaagg cagcagcaac aacggcagca gcagcagcaa cagcagcaac agcaagaata
     5041 ccaggaactg ttcaggcaca tgaaccaagg ggatgcgggg agtctggctc ccagccttgg
     5101 gggacagagc atgacagagc gccaggcttt atcttatcaa aatgctgact cttatcacca
     5161 tcacaccagc ccccagcatc tgctacaaat cagggcacaa gaatgtgtct cacaggcttc
     5221 ctcacccacc ccgccccacg ggtatgctca ccagccggca ctgatgcatt cagagagcat
     5281 ggaggaggac tgctcgtgtg agggggccaa ggatggcttc caagacagta agagttcaag
     5341 tacattgacc aaaggttgcc atgacagccc tctgctcttg agtaccggtg gacctgggga
     5401 ccctgaatct ttgctaggaa ctgtgagtca tgcccaagaa ttggggatac atccctatgg
     5461 tcatcagcca actgctgcat tcagtaaaaa taaggtgccc agcagagagc ctgtcatagg
     5521 gaactgcatg gatagaagtt ctccaggaca agcagtggag ctgccggatc acaatgggct
     5581 cgggtaccca gcacgcccct ccgtccatga gcaccacagg ccccgggccc tccagagaca
     5641 ccacacgatc cagaacagcg acgatgctta tgtacagctg gataacttgc caggaatgag
     5701 tctcgtggct gggaaagcac ttagctctgc ccggatgtcg gatgcagttc tcagtcagtc
     5761 ttcgctcatg ggcagccagc agtttcagga tggggaaaat gaggaatgtg gggcaagcct
     5821 gggaggtcat gagcacccag acctgagtga tggcagccag catttaaact cctcttgcta
     5881 tccatctacg tgtattacag acattctgct cagctacaag caccccgaag tctccttcag
     5941 catggagcag gcaggcgtgg gcagcggcgc caccaacttc agcctgctga agcaggccgg
     6001 cgacgtggag gagaaccccg gccccatgac cgagtacaag cccatgattg aacaagatgg
     6061 attgcacgca ggttctccgg ccgcttgggt ggagaggcta ttcggctatg actgggcaca
     6121 acagacaatc ggctgctctg atgccgccgt gttccggctg tcagcgcagg ggcgcccggt
     6181 tctttttgtc aagaccgacc tgtccggtgc cctgaatgaa ctgcaggacg aggcagcgcg
     6241 gctatcgtgg ctggccacga cgggcgttcc ttgcgcagct gtgctcgacg ttgtcactga
     6301 agcgggaagg gactggctgc tattgggcga agtgccgggg caggatctcc tgtcatctca
     6361 ccttgctcct gccgagaaag tatccatcat ggctgatgca atgcggcggc tgcatacgct
     6421 tgatccggct acctgcccat tcgaccacca agcgaaacat cgcatcgagc gagcacgtac
     6481 tcggatggaa gccggtcttg tcgatcagga tgatctggac gaagagcatc aggggctcgc
     6541 gccagccgaa ctgttcgcca ggctcaaggc gcgcatgccc gacggcgagg atctcgtcgt
     6601 gacccatggc gatgcctgct tgccgaatat catggtggaa aatggccgct tttctggatt
     6661 catcgactgt ggccggctgg gtgtggcgga ccgctatcag gacatagcgt tggctacccg
     6721 tgatattgct gaagagcttg gcggcgaatg ggctgaccgc ttcctcgtgc tttacggtat
     6781 cgccgctccc gattcgcagc gcatcgcctt ctatcgcctt cttgacgagt tcttctgaac
     6841 gcgttaagtc gacaatcaac ctctggatta caaaatttgt gaaagattga ctggtattct
     6901 taactatgtt gctcctttta cgctatgtgg atacgctgct ttaatgcctt tgtatcatgc
     6961 tattgcttcc cgtatggctt tcattttctc ctccttgtat aaatcctggt tgctgtctct
     7021 ttatgaggag ttgtggcccg ttgtcaggca acgtggcgtg gtgtgcactg tgtttgctga
     7081 cgcaaccccc actggttggg gcattgccac cacctgtcag ctcctttccg ggactttcgc
     7141 tttccccctc cctattgcca cggcggaact catcgccgcc tgccttgccc gctgctggac
     7201 aggggctcgg ctgttgggca ctgacaattc cgtggtgttg tcggggaaat catcgtcctt
     7261 tccttggctg ctcgcctgtg ttgccacctg gattctgcgc gggacgtcct tctgctacgt
     7321 cccttcggcc ctcaatccag cggaccttcc ttcccgcggc ctgctgccgg ctctgcggcc
     7381 tcttccgcgt cttcgccttc gccctcagac gagtcggatc tccctttggg ccgcctcccc
     7441 gcgtcgactt taagaccaat gacttacaag gcagctgtag atcttagcca ctttttaaaa
     7501 gaaaaggggg gactggaagg gctaattcac tcccaacgaa gacaagatct gctttttgct
     7561 tgtactgggt ctctctggtt agaccagatc tgagcctggg agctctctgg ctaactaggg
     7621 aacccactgc ttaagcctca ataaagcttg ccttgagtgc ttcaagtagt gtgtgcccgt
     7681 ctgttgtgtg actctggtaa ctagagatcc ctcagaccct tttagtcagt gtggaaaatc
     7741 tctagcagta cgtatagtag ttcatgtcat cttattattc agtatttata acttgcaaag
     7801 aaatgaatat cagagagtga gaggaacttg tttattgcag cttataatgg ttacaaataa
     7861 agcaatagca tcacaaattt cacaaataaa gcattttttt cactgcattc tagttgtggt
     7921 ttgtccaaac tcatcaatgt atcttatcat gtctggctct agctatcccg cccctaactc
     7981 cgcccatccc gcccctaact ccgcccagtt ccgcccattc tccgccccat ggctgactaa
     8041 ttttttttat ttatgcagag gccgaggccg cctcggcctc tgagctattc cagaagtagt
     8101 gaggaggctt ttttggaggc ctagggacgt acccaattcg ccctatagtg agtcgtatta
     8161 cgcgcgctca ctggccgtcg ttttacaacg tcgtgactgg gaaaaccctg gcgttaccca
     8221 acttaatcgc cttgcagcac atcccccttt cgccagctgg cgtaatagcg aagaggcccg
     8281 caccgatcgc ccttcccaac agttgcgcag cctgaatggc gaatgggacg cgccctgtag
     8341 cggcgcatta agcgcggcgg gtgtggtggt tacgcgcagc gtgaccgcta cacttgccag
     8401 cgccctagcg cccgctcctt tcgctttctt cccttccttt ctcgccacgt tcgccggctt
     8461 tccccgtcaa gctctaaatc gggggctccc tttagggttc cgatttagtg ctttacggca
     8521 cctcgacccc aaaaaacttg attagggtga tggttcacgt agtgggccat cgccctgata
     8581 gacggttttt cgccctttga cgttggagtc cacgttcttt aatagtggac tcttgttcca
     8641 aactggaaca acactcaacc ctatctcggt ctattctttt gatttataag ggattttgcc
     8701 gatttcggcc tattggttaa aaaatgagct gatttaacaa aaatttaacg cgaattttaa
     8761 caaaatatta acgcttacaa tttaggtggc acttttcggg gaaatgtgcg cggaacccct
     8821 atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga
     8881 taaatgcttc aataatattg aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc
     8941 cttattccct tttttgcggc attttgcctt cctgtttttg ctcacccaga aacgctggtg
     9001 aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg gttacatcga actggatctc
     9061 aacagcggta agatccttga gagttttcgc cccgaagaac gttttccaat gatgagcact
     9121 tttaaagttc tgctatgtgg cgcggtatta tcccgtattg acgccgggca agagcaactc
     9181 ggtcgccgca tacactattc tcagaatgac ttggttgagt actcaccagt cacagaaaag
     9241 catcttacgg atggcatgac agtaagagaa ttatgcagtg ctgccataac catgagtgat
     9301 aacactgcgg ccaacttact tctgacaacg atcggaggac cgaaggagct aaccgctttt
     9361 ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa
     9421 gccataccaa acgacgagcg tgacaccacg atgcctgtag caatggcaac aacgttgcgc
     9481 aaactattaa ctggcgaact acttactcta gcttcccggc aacaattaat agactggatg
     9541 gaggcggata aagttgcagg accacttctg cgctcggccc ttccggctgg ctggtttatt
     9601 gctgataaat ctggagccgg tgagcgtggg tctcgcggta tcattgcagc actggggcca
     9661 gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagtcaggc aactatggat
     9721 gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg gtaactgtca
     9781 gaccaagttt actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg
     9841 atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg
     9901 ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt
     9961 ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg
    10021 ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata
    10081 ccaaatactg ttcttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca
    10141 ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag
    10201 tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc
    10261 tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga
    10321 tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg
    10381 tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac
    10441 gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg
    10501 tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg
    10561 ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct
    10621 gtggataacc gtattaccgc ctttgagtga gctgataccg ctcgccgcag ccgaacgacc
    10681 gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc caatacgcaa accgcctctc
    10741 cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca ggtttcccga ctggaaagcg
    10801 ggcagtgagc gcaacgcaat taatgtgagt tagctcactc attaggcacc ccaggcttta
    10861 cactttatgc ttccggctcg tatgttgtgt ggaattgtga gcggataaca atttcacaca
    10921 ggaaacagct atgaccatga ttacgccaag cgcgcaatta accctcacta aagggaacaa
    10981 aagctggag
//
