LOCUS       sequence-208152-        2312 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  AAV-mediated expression of mTurquoise2 under the TRE promoter..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2312)
  AUTHORS   Sakaguchi R, Leiwe MN, Imai T
  TITLE     Bright multicolor labeling of neuronal circuits with fluorescent 
            proteins and chemical tags.
  JOURNAL   eLife. 2018 Nov 20;7. pii: 40350. doi: 10.7554/eLife.40350.
  PUBMED    30454553
REFERENCE   2  (bases 1 to 2312)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "eLife."; 
            date: "2018-11-20"; volume: "7. pii: 40350. doi"; pages: " 
            10.7554/eLife.40350"
COMMENT     Sequence Label: pAAV-TRE-mTurquoise2-WPRE
FEATURES             Location/Qualifiers
     source          1..2312
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     repeat_region   2..131
                     /label=AAV2 ITR
     protein_bind    205..223
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    241..259
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    276..294
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    312..330
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    348..366
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    383..401
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    419..437
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     promoter        449..487
                     /label=minimal CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     484..508
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     regulatory      522..531
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(528..530,531..533,534..1247)
                     /codon_start=1
                     /product="enhanced monomeric variant of CFP (Goedhart et 
                     al., 2012)"
                     /label=mTurquoise2
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLSWGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYFSDNVYITADKQKNGIK
                     ANFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(573..594)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(834..853)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     1181..1202
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     misc_feature    1261..1849
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(1314..1334)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(1732..1743)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(1852..1868)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1853..1869)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     polyA_signal    1898..2019
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(1909..1928)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     1963..1982
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     repeat_region   2181..2310
                     /label=AAV2 ITR
ORIGIN
        1 gctgcgcgct cgctcgctca ctgaggccgc ccgggcaaag cccgggcgtc gggcgacctt
       61 tggtcgcccg gcctcagtga gcgagcgagc gcgcagagag ggagtggcca actccatcac
      121 taggggttcc ttgtagttaa tgattaaccc gccatgctac ttatctacgt agccatgctc
      181 taggaagatc tcgaattggt ttactcccta tcagtgatag agaacgtatg tcgagtttac
      241 tccctatcag tgatagagaa cgatgtcgag tttactccct atcagtgata gagaacgtat
      301 gtcgagttta ctccctatca gtgatagaga acgtatgtcg agtttactcc ctatcagtga
      361 tagagaacgt atgtcgagtt tatccctatc agtgatagag aacgtatgtc gagtttactc
      421 cctatcagtg atagagaacg tatgtcgagg taggcgtgta cggtgggagg cctatataag
      481 cagagctcgt ttagtgaacc gtcagatcgc ctggagaatt cgccaccatg gtgagcaagg
      541 gcgaggagct gttcaccggg gtggtgccca tcctggtcga gctggacggc gacgtaaacg
      601 gccacaagtt cagcgtgtcc ggcgagggcg agggcgatgc cacctacggc aagctgaccc
      661 tgaagttcat ctgcaccacc ggcaagctgc ccgtgccctg gcccaccctc gtgaccaccc
      721 tgtcctgggg cgtgcagtgc ttcgcccgct accccgacca catgaagcag cacgacttct
      781 tcaagtccgc catgcccgaa ggctacgtcc aggagcgcac catcttcttc aaggacgacg
      841 gcaactacaa gacccgcgcc gaggtgaagt tcgagggcga caccctggtg aaccgcatcg
      901 agctgaaggg catcgacttc aaggaggacg gcaacatcct ggggcacaag ctggagtaca
      961 actactttag cgacaacgtc tatatcaccg ccgacaagca gaagaacggc atcaaggcca
     1021 acttcaagat ccgccacaac atcgaggacg gcggcgtgca gctcgccgac cactaccagc
     1081 agaacacccc catcggcgac ggccccgtgc tgctgcccga caaccactac ctgagcaccc
     1141 agtccaagct gagcaaagac cccaacgaga agcgcgatca catggtcctg ctggagttcg
     1201 tgaccgccgc cgggatcact ctcggcatgg acgagctgta caagtaagaa gcttatcgat
     1261 aatcaacctc tggattacaa aatttgtgaa agattgactg gtattcttaa ctatgttgct
     1321 ccttttacgc tatgtggata cgctgcttta atgcctttgt atcatgctat tgcttcccgt
     1381 atggctttca ttttctcctc cttgtataaa tcctggttgc tgtctcttta tgaggagttg
     1441 tggcccgttg tcaggcaacg tggcgtggtg tgcactgtgt ttgctgacgc aacccccact
     1501 ggttggggca ttgccaccac ctgtcagctc ctttccggga ctttcgcttt ccccctccct
     1561 attgccacgg cggaactcat cgccgcctgc cttgcccgct gctggacagg ggctcggctg
     1621 ttgggcactg acaattccgt ggtgttgtcg gggaaatcat cgtcctttcc ttggctgctc
     1681 gcctgtgttg ccacctggat tctgcgcggg acgtccttct gctacgtccc ttcggccctc
     1741 aatccagcgg accttccttc ccgcggcctg ctgccggctc tgcggcctct tccgcgtctt
     1801 cgccttcgcc ctcagacgag tcggatctcc ctttgggccg cctccccgca tcgataccgt
     1861 cgacctcgac ccgggcggcc gcttcgagca gacatgataa gatacattga tgagtttgga
     1921 caaaccacaa ctagaatgca gtgaaaaaaa tgctttattt gtgaaatttg tgatgctatt
     1981 gctttatttg taaccattat aagctgcaat aaacaagtta acaacaacaa ttgcattcat
     2041 tttatgtttc aggttcaggg ggagatgtgg gaggtttttt aaagcaagta aaacctctac
     2101 aaatgtggta aaatcgataa ggatcttcct agagcatggc tacgtagata agtagcatgg
     2161 cgggttaatc attaactaca aggaacccct agtgatggag ttggccactc cctctctgcg
     2221 cgctcgctcg ctcactgagg ccgggcgacc aaaggtcgcc cgacgcccgg gctttgcccg
     2281 ggcggcctca gtgagcgagc gagcgcgcag ct
//
