LOCUS       sequence-208157-        3022 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  AAV-mediated expression of tdTomato under the TRE promoter..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 3022)
  AUTHORS   Sakaguchi R, Leiwe MN, Imai T
  TITLE     Bright multicolor labeling of neuronal circuits with fluorescent 
            proteins and chemical tags.
  JOURNAL   eLife. 2018 Nov 20;7. pii: 40350. doi: 10.7554/eLife.40350.
  PUBMED    30454553
REFERENCE   2  (bases 1 to 3022)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "eLife."; 
            date: "2018-11-20"; volume: "7. pii: 40350. doi"; pages: " 
            10.7554/eLife.40350"
COMMENT     Sequence Label: pAAV-TRE-tdTomato-WPRE
FEATURES             Location/Qualifiers
     source          1..3022
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     repeat_region   2..131
                     /label=AAV2 ITR
     protein_bind    205..223
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    241..259
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    276..294
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    312..330
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    348..366
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    383..401
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    419..437
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     promoter        449..487
                     /label=minimal CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     484..508
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     regulatory      522..531
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             528..1958
                     /codon_start=1
                     /product="tandem dimeric (pseudo-monomeric) derivative of 
                     DsRed (Shaner et al., 2004)"
                     /label=tdTomato
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEVIKEFMRFKVRMEGSMNGHEFEIEGEGEGRPYEGTQTA
                     KLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYKKLSFPEGFKWERVMNFEDGG
                     LVTVTQDSSLQDGTLIYKVKMRGTNFPPDGPVMQKKTMGWEASTERLYPRDGVLKGEIH
                     QALKLKDGGHYLVEFKTIYMAKKPVQLPGYYYVDTKLDITSHNEDYTIVEQYERSEGRH
                     HLFLGHGTGSTGSGSSGTASSEDNNMAVIKEFMRFKVRMEGSMNGHEFEIEGEGEGRPY
                     EGTQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYKKLSFPEGFKWERVM
                     NFEDGGLVTVTQDSSLQDGTLIYKVKMRGTNFPPDGPVMQKKTMGWEASTERLYPRDGV
                     LKGEIHQALKLKDGGHYLVEFKTIYMAKKPVQLPGYYYVDTKLDITSHNEDYTIVEQYE
                     RSEGRHHLFLYGMDELYK"
     misc_feature    1972..2560
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(2025..2045)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(2443..2454)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(2563..2579)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2564..2580)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     polyA_signal    2609..2730
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2620..2639)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     2674..2693
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     repeat_region   2892..3021
                     /label=AAV2 ITR
ORIGIN
        1 gctgcgcgct cgctcgctca ctgaggccgc ccgggcaaag cccgggcgtc gggcgacctt
       61 tggtcgcccg gcctcagtga gcgagcgagc gcgcagagag ggagtggcca actccatcac
      121 taggggttcc ttgtagttaa tgattaaccc gccatgctac ttatctacgt agccatgctc
      181 taggaagatc tcgaattggt ttactcccta tcagtgatag agaacgtatg tcgagtttac
      241 tccctatcag tgatagagaa cgatgtcgag tttactccct atcagtgata gagaacgtat
      301 gtcgagttta ctccctatca gtgatagaga acgtatgtcg agtttactcc ctatcagtga
      361 tagagaacgt atgtcgagtt tatccctatc agtgatagag aacgtatgtc gagtttactc
      421 cctatcagtg atagagaacg tatgtcgagg taggcgtgta cggtgggagg cctatataag
      481 cagagctcgt ttagtgaacc gtcagatcgc ctggagaatt cgccaccatg gtgagcaagg
      541 gcgaggaggt catcaaagag ttcatgcgct tcaaggtgcg catggagggc tccatgaacg
      601 gccacgagtt cgagatcgag ggcgagggcg agggccgccc ctacgagggc acccagaccg
      661 ccaagctgaa ggtgaccaag ggcggccccc tgcccttcgc ctgggacatc ctgtcccccc
      721 agttcatgta cggctccaag gcgtacgtga agcaccccgc cgacatcccc gattacaaga
      781 agctgtcctt ccccgagggc ttcaagtggg agcgcgtgat gaacttcgag gacggcggtc
      841 tggtgaccgt gacccaggac tcctccctgc aggacggcac gctgatctac aaggtgaaga
      901 tgcgcggcac caacttcccc cccgacggcc ccgtaatgca gaagaagacc atgggctggg
      961 aggcctccac cgagcgcctg tacccccgcg acggcgtgct gaagggcgag atccaccagg
     1021 ccctgaagct gaaggacggc ggccactacc tggtggagtt caagaccatc tacatggcca
     1081 agaagcccgt gcaactgccc ggctactact acgtggacac caagctggac atcacctccc
     1141 acaacgagga ctacaccatc gtggaacagt acgagcgctc cgagggccgc caccacctgt
     1201 tcctggggca tggcaccggc agcaccggca gcggcagctc cggcaccgcc tcctccgagg
     1261 acaacaacat ggccgtcatc aaagagttca tgcgcttcaa ggtgcgcatg gagggctcca
     1321 tgaacggcca cgagttcgag atcgagggcg agggcgaggg ccgcccctac gagggcaccc
     1381 agaccgccaa gctgaaggtg accaagggcg gccccctgcc cttcgcctgg gacatcctgt
     1441 ccccccagtt catgtacggc tccaaggcgt acgtgaagca ccccgccgac atccccgatt
     1501 acaagaagct gtccttcccc gagggcttca agtgggagcg cgtgatgaac ttcgaggacg
     1561 gcggtctggt gaccgtgacc caggactcct ccctgcagga cggcacgctg atctacaagg
     1621 tgaagatgcg cggcaccaac ttcccccccg acggccccgt aatgcagaag aagaccatgg
     1681 gctgggaggc ctccaccgag cgcctgtacc cccgcgacgg cgtgctgaag ggcgagatcc
     1741 accaggccct gaagctgaag gacggcggcc actacctggt ggagttcaag accatctaca
     1801 tggccaagaa gcccgtgcaa ctgcccggct actactacgt ggacaccaag ctggacatca
     1861 cctcccacaa cgaggactac accatcgtgg aacagtacga gcgctccgag ggccgccacc
     1921 acctgttcct gtacggcatg gacgagctgt acaagtaaga agcttatcga taatcaacct
     1981 ctggattaca aaatttgtga aagattgact ggtattctta actatgttgc tccttttacg
     2041 ctatgtggat acgctgcttt aatgcctttg tatcatgcta ttgcttcccg tatggctttc
     2101 attttctcct ccttgtataa atcctggttg ctgtctcttt atgaggagtt gtggcccgtt
     2161 gtcaggcaac gtggcgtggt gtgcactgtg tttgctgacg caacccccac tggttggggc
     2221 attgccacca cctgtcagct cctttccggg actttcgctt tccccctccc tattgccacg
     2281 gcggaactca tcgccgcctg ccttgcccgc tgctggacag gggctcggct gttgggcact
     2341 gacaattccg tggtgttgtc ggggaaatca tcgtcctttc cttggctgct cgcctgtgtt
     2401 gccacctgga ttctgcgcgg gacgtccttc tgctacgtcc cttcggccct caatccagcg
     2461 gaccttcctt cccgcggcct gctgccggct ctgcggcctc ttccgcgtct tcgccttcgc
     2521 cctcagacga gtcggatctc cctttgggcc gcctccccgc atcgataccg tcgacctcga
     2581 cccgggcggc cgcttcgagc agacatgata agatacattg atgagtttgg acaaaccaca
     2641 actagaatgc agtgaaaaaa atgctttatt tgtgaaattt gtgatgctat tgctttattt
     2701 gtaaccatta taagctgcaa taaacaagtt aacaacaaca attgcattca ttttatgttt
     2761 caggttcagg gggagatgtg ggaggttttt taaagcaagt aaaacctcta caaatgtggt
     2821 aaaatcgata aggatcttcc tagagcatgg ctacgtagat aagtagcatg gcgggttaat
     2881 cattaactac aaggaacccc tagtgatgga gttggccact ccctctctgc gcgctcgctc
     2941 gctcactgag gccgggcgac caaaggtcgc ccgacgcccg ggctttgccc gggcggcctc
     3001 agtgagcgag cgagcgcgca gc
//
