LOCUS       sequence-208159-        8628 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Homology arms, promoter and linker-mTagRFPT-CAAX sequence for 
            internal insertion of CAGGS-driven mTagRFPT-CAAX at the AAVS1 safe 
            harbor location in human cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8628)
  AUTHORS   Allen Institute for Cell Science
  TITLE     Allen Institute for Cell Science Plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8628)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: AICSDP-42:AAVS1-mTagRFPT-CAAX
FEATURES             Location/Qualifiers
     source          1..8628
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     intron          149..1166
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     1090..1112
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     1174..1193
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     regulatory      1222..1231
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1228..1959
                     /codon_start=1
                     /product="photostable variant of TagRFP (Shaner et al., 
                     2008)"
                     /label=TagRFP-T
                     /translation="MVSKGEELIKENMHMKLYMEGTVNNHHFKCTSEGEGKPYEGTQTM
                     RIKVVEGGPLPFAFDILATSFMYGSRTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGG
                     VLTATQDTSLQDGCLIYNVKIRGVNFPSNGPVMQKKTLGWEANTEMLYPADGGLEGRTD
                     MALKLVGGGHLICNFKTTYRSKKPAKNLKMPGVYYVDHRLERIKEADKETYVEQHEVAV
                     ARYCDLPSKLGHKLNGMDELYK"
     primer_bind     complement(2148..2167)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    2213..2268
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(2267..2286)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(2629..2645)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    2653..2669
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(2677..2683,2684..2701,2702..2707))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2722..2743
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     misc_feature    2817..3653
                     /label=HA-R
                     /note="right homology arm from the adeno-associated virus 
                     integration site (AAVS1) within intron 1 of the human 
                     PPP1R12C gene"
     primer_bind     complement(3675..3692)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(3675..3691)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3684..3706)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(3900..3919)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     4019..4041
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(4079..4097)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        4165..4269
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4270..4338,4339..5130)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4488..4507)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5301..5889
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5790..5809
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6043..6060
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    6177..6198
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(6213..6218,6219..6236,6237..6243)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6251..6267
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     6275..6291
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     misc_feature    6512..7013
                     /label=HA-L
                     /note="left homology arm from the adeno-associated virus 
                     integration site (AAVS1) within intron 1 of the human 
                     PPP1R12C gene"
     misc_feature    7140..7165
                     /label=SA
                     /note="splice acceptor site"
     CDS             7189..7242
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             7252..7851
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(7252..7271)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     7748..7768
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     primer_bind     complement(7883..7900)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    7889..8113
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     enhancer        8119..8498
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        join(8500..8628,1..147)
                     /label=chicken beta-actin promoter
ORIGIN
        1 ggcggggcgg ggcggggcga ggggcggggc ggggcgaggc ggagaggtgc ggcggcagcc
       61 aatcagagcg gcgcgctccg aaagtttcct tttatggcga ggcggcggcg gcggcggccc
      121 tataaaaagc gaagcgcgcg gcgggcgggg agtcgctgcg acgctgcctt cgccccgtgc
      181 cccgctccgc cgccgcctcg cgccgcccgc cccggctctg actgaccgcg ttactcccac
      241 aggtgagcgg gcgggacggc ccttctcctc cgggctgtaa ttagcgcttg gtttaatgac
      301 ggcttgtttc ttttctgtgg ctgcgtgaaa gccttgaggg gctccgggag ggccctttgt
      361 gcggggggag cggctcgggg ggtgcgtgcg tgtgtgtgtg cgtggggagc gccgcgtgcg
      421 gctccgcgct gcccggcggc tgtgagcgct gcgggcgcgg cgcggggctt tgtgcgctcc
      481 gcagtgtgcg cgaggggagc gcggccgggg gcggtgcccc gcggtgcggg gggggctgcg
      541 aggggaacaa aggctgcgtg cggggtgtgt gcgtgggggg gtgagcaggg ggtgtgggcg
      601 cgtcggtcgg gctgcaaccc cccctgcacc cccctccccg agttgctgag cacggcccgg
      661 cttcgggtgc ggggctccgt acggggcgtg gcgcggggct cgccgtgccg ggcggggggt
      721 ggcggcaggt gggggtgccg ggcggggcgg ggccgcctcg ggccggggag ggctcggggg
      781 aggggcgcgg cggcccccgg agcgccggcg gctgtcgagg cgcggcgagc cgcagccatt
      841 gccttttatg gtaatcgtgc gagagggcgc agggacttcc tttgtcccaa atctgtgcgg
      901 agccgaaatc tgggaggcgc cgccgcaccc cctctagcgg gcgcggggcg aagcggtgcg
      961 gcgccggcag gaaggaaatg ggcggggagg gccttcgtgc gtcgccgcgc cgccgtcccc
     1021 ttctccctct ccagcctcgg ggctgtccgc ggggggacgg ctgccttcgg gggggacggg
     1081 gcagggcggg gttcggcttc tggcgtgtga ccggcggctc tagagcctct gctaaccatg
     1141 ttcatgcctt cttctttttc ctacagctcc tgggcaacgt gctggttatt gtgctgtctc
     1201 atcattttgg caaagaattc cgccaccatg gtgtctaagg gcgaagagct gattaaggag
     1261 aacatgcaca tgaagctgta catggagggc accgtgaaca accaccactt caagtgcaca
     1321 tccgagggcg aaggcaagcc ctacgagggc acccagacca tgagaatcaa ggtggtcgag
     1381 ggcggccctc tccccttcgc cttcgacatc ctggctacca gcttcatgta cggcagcaga
     1441 accttcatca accacaccca gggcatcccc gatttcttta agcagtcctt ccctgagggc
     1501 ttcacatggg agagagtcac cacatacgaa gacgggggcg tgctgaccgc tacccaggac
     1561 accagcctcc aggacggctg cctcatctac aacgtcaaga tcagaggggt gaacttccca
     1621 tccaacggcc ctgtgatgca gaagaaaaca ctcggctggg aggccaacac cgagatgctg
     1681 taccccgctg acggcggcct ggaaggcaga accgacatgg ccctgaagct cgtgggcggg
     1741 ggccacctga tctgcaactt caagaccaca tacagatcca agaaacccgc taagaacctc
     1801 aagatgcccg gcgtctacta tgtggaccac agactggaaa gaatcaagga ggccgacaaa
     1861 gagacctacg tcgagcagca cgaggtggct gtggccagat actgcgacct ccctagcaaa
     1921 ctggggcaca aacttaatgg catggacgag ctgtacaagt ccggactcag atctcgagct
     1981 caagcttcga attcaaagat gagcaaagat ggtaaaaaga agaaaaagaa gtcaaagaca
     2041 aagtgtgtaa ttatgtaaac gcgtgaattc actcctcagg tgcaggctgc ctatcagaag
     2101 gtggtggctg gtgtggccaa tgccctggct cacaaatacc actgagatct ttttccctct
     2161 gccaaaaatt atggggacat catgaagccc cttgagcatc tgacttctgg ctaataaagg
     2221 aaatttattt tcattgcaat agtgtgttgg aattttttgt gtctctcact cggaaggaca
     2281 tatgggaggg caaatcattt aaaacatcag aatgagtatt tggtttagag tttggcaaca
     2341 tatgcccata tgctggctgc catgaacaaa ggttggctat aaagaggtca tcagtatatg
     2401 aaacagcccc ctgctgtcca ttccttattc catagaaaag ccttgacttg aggttagatt
     2461 ttttttatat tttgttttgt gttatttttt tctttaacat ccctaaaatt ttccttacat
     2521 gttttactag ccagattttt cctcctctcc tgactactcc cagtcatagc tgtccctctt
     2581 ctcttatgga gatccctcga cctgcagccc aagcttggcg taatcatggt catagctgtt
     2641 tcctgtgtga aattgttatc cgctcacaat tccacacaac atacgagccg gaagcataaa
     2701 gtgtaaagcc tggggtgcct aatgagtgag ctaactcaca ttaattgcgt tgcgctcact
     2761 gcccgctttc cagtcgggaa acctgtcgtg ccagcggatc gacagtacta agctttacta
     2821 gggacaggat tggtgacaga aaagccccat ccttaggcct cctccttcct agtctcctga
     2881 tattgggtct aacccccacc tcctgttagg cagattcctt atctggtgac acacccccat
     2941 ttcctggagc catctctctc cttgccagaa cctctaaggt ttgcttacga tggagccaga
     3001 gaggatcctg ggagggagag cttggcaggg ggtgggaggg aaggggggga tgcgtgacct
     3061 gcccggttct cagtggccac cctgcgctac cctctcccag aacctgagct gctctgacgc
     3121 ggctgtctgg tgcgtttcac tgatcctggt gctgcagctt ccttacactt cccaagagga
     3181 gaagcagttt ggaaaaacaa aatcagaata agttggtcct gagttctaac tttggctctt
     3241 cacctttcta gtccccaatt tatattgttc ctccgtgcgt cagttttacc tgtgagataa
     3301 ggccagtagc cagccccgtc ctggcagggc tgtggtgagg aggggggtgt ccgtgtggaa
     3361 aactcccttt gtgagaatgg tgcgtcctag gtgttcacca ggtcgtggcc gcctctactc
     3421 cctttctctt tctccatcct tctttcctta aagagtcccc agtgctatct gggacatatt
     3481 cctccgccca gagcagggtc ccgcttccct aaggccctgc tctgggcttc tgggtttgag
     3541 tccttggcaa gcccaggaga ggcgctcagg cttccctgtc ccccttcctc gtccaccatc
     3601 tcatgcccct ggctctcctg ccccttccct acaggggttc ctggctctgc tctatcccaa
     3661 tggcgcgtca attcactggc cgtcgtttta caacgtcgtg actgggaaaa ccctggcgtt
     3721 acccaactta atcgccttgc agcacatccc cctttcgcca gctggcgtaa tagcgaagag
     3781 gcccgcaccg atcgcccttc ccaacagttg cgcagcctga atggcgaatg gcgcctgatg
     3841 cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatggtg cactctcagt
     3901 acaatctgct ctgatgccgc atagttaagc cagccccgac acccgccaac acccgctgac
     3961 gcgccctgac gggcttgtct gctcccggca tccgcttaca gacaagctgt gaccgtctcc
     4021 gggagctgca tgtgtcagag gttttcaccg tcatcaccga aacgcgcgag acgaaagggc
     4081 ctcgtgatac gcctattttt ataggttaat gtcatgataa taatggtttc ttagacgtca
     4141 ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat
     4201 tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa
     4261 aggaagagta tgagtattca acatttccgt gtcgccctta ttcccttttt tgcggcattt
     4321 tgccttcctg tttttgctca cccagaaacg ctggtgaaag taaaagatgc tgaagatcag
     4381 ttgggtgcac gagtgggtta catcgaactg gatctcaaca gcggtaagat ccttgagagt
     4441 tttcgccccg aagaacgttt tccaatgatg agcactttta aagttctgct atgtggcgcg
     4501 gtattatccc gtattgacgc cgggcaagag caactcggtc gccgcataca ctattctcag
     4561 aatgacttgg ttgagtactc accagtcaca gaaaagcatc ttacggatgg catgacagta
     4621 agagaattat gcagtgctgc cataaccatg agtgataaca ctgcggccaa cttacttctg
     4681 acaacgatcg gaggaccgaa ggagctaacc gcttttttgc acaacatggg ggatcatgta
     4741 actcgccttg atcgttggga accggagctg aatgaagcca taccaaacga cgagcgtgac
     4801 accacgatgc ctgtagcaat ggcaacaacg ttgcgcaaac tattaactgg cgaactactt
     4861 actctagctt cccggcaaca attaatagac tggatggagg cggataaagt tgcaggacca
     4921 cttctgcgct cggcccttcc ggctggctgg tttattgctg ataaatctgg agccggtgag
     4981 cgtgggtctc gcggtatcat tgcagcactg gggccagatg gtaagccctc ccgtatcgta
     5041 gttatctaca cgacggggag tcaggcaact atggatgaac gaaatagaca gatcgctgag
     5101 ataggtgcct cactgattaa gcattggtaa ctgtcagacc aagtttactc atatatactt
     5161 tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat
     5221 aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta
     5281 gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa
     5341 acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt
     5401 tttccgaagg taactggctt cagcagagcg cagataccaa atactgttct tctagtgtag
     5461 ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta
     5521 atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca
     5581 agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag
     5641 cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa
     5701 agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga
     5761 acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc
     5821 gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc
     5881 ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt
     5941 gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccttt
     6001 gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagcgag
     6061 gaagcggaag agcgcccaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa
     6121 tgcagctggc acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat
     6181 gtgagttagc tcactcatta ggcaccccag gctttacact ttatgcttcc ggctcgtatg
     6241 ttgtgtggaa ttgtgagcgg ataacaattt cacacaggaa acagctatga ccatgattac
     6301 gccaagctcg gcgcgtattg ggatcccttt gctttctctg accagcattc tctcccctgg
     6361 gcctgtgccg ctttctgtct gcagcttgtg gcctgggtca cctctacggc tggcccagat
     6421 ccttccctgc cgcctccttc aggttccgtc ttcctccact ccctcttccc cttgctctct
     6481 gctgtgttgc tgcccaagga tgctctttcc ggagcacttc cttctcggcg ctgcaccacg
     6541 tgatgtcctc tgagcggatc ctccccgtgt ctgggtcctc tccgggcatc tctcctccct
     6601 cacccaaccc catgccgtct tcactcgctg ggttcccttt tccttctcct tctggggcct
     6661 gtgccatctc tcgtttctta ggatggcctt ctccgacgga tgtctccctt gcgtcccgcc
     6721 tccccttctt gtaggcctgc atcatcaccg tttttctgga caaccccaaa gtaccccgtc
     6781 tccctggctt tagccacctc tccatcctct tgctttcttt gcctggacac cccgttctcc
     6841 tgtggattcg ggtcacctct cactcctttc atttgggcag ctcccctacc ccccttacct
     6901 ctctagtctg tgctagctct tccagccccc tgtcatggca tcttccaggg gtccgagagc
     6961 tcagctagtc ttcttcctcc aacccgggcc cctatgtcca cttcaggaca gcatgtttgc
     7021 tgcctccagg gatcctgtgt ccccgagctg ggaccacctt atattcccag ggccggttaa
     7081 tgtggctctg gttctgggta cttttatctg tcccctccac cccacagtgg gccaagcttc
     7141 tgacctcttc tcttcctccc acagggcctc gagagatctg gcagcggaga gggcagagga
     7201 agtcttctaa catgcggtga cgtggaggag aatcccggcc ctaggctcga gatgaccgag
     7261 tacaagccca cggtgcgcct cgccacccgc gacgacgtcc ccagggccgt acgcaccctc
     7321 gccgccgcgt tcgccgacta ccccgccacg cgccacaccg tcgatccgga ccgccacatc
     7381 gagcgggtca ccgagctgca agaactcttc ctcacgcgcg tcgggctcga catcggcaag
     7441 gtgtgggtcg cggacgacgg cgccgcggtg gcggtctgga ccacgccgga gagcgtcgaa
     7501 gcgggggcgg tgttcgccga gatcggcccg cgcatggccg agttgagcgg ttcccggctg
     7561 gccgcgcagc aacagatgga aggcctcctg gcgccgcacc ggcccaagga gcccgcgtgg
     7621 ttcctggcca ccgtcggcgt ctcgcccgac caccagggca agggtctggg cagcgccgtc
     7681 gtgctccccg gagtggaggc ggccgagcgc gccggggtgc ccgccttcct ggagacctcc
     7741 gcgccccgca acctcccctt ctacgagcgg ctcggcttca ccgtcaccgc cgacgtcgag
     7801 gtgcccgaag gaccgcgcac ctggtgcatg acccgcaagc ccggtgcctg atctagaggg
     7861 cccgtttaaa cccgctgatc agcctcgact gtgccttcta gttgccagcc atctgttgtt
     7921 tgcccctccc ccgtgccttc cttgaccctg gaaggtgcca ctcccactgt cctttcctaa
     7981 taaaatgagg aaattgcatc gcattgtctg agtaggtgtc attctattct ggggggtggg
     8041 gtggggcagg acagcaaggg ggaggattgg gaagacaata gcaggcatgc tggggatgcg
     8101 gtgggctcta tgggtctcga cattgattat tgactagtta ttaatagtaa tcaattacgg
     8161 ggtcattagt tcatagccca tatatggagt tccgcgttac ataacttacg gtaaatggcc
     8221 cgcctggctg accgcccaac gacccccgcc cattgacgtc aataatgacg tatgttccca
     8281 tagtaacgcc aatagggact ttccattgac gtcaatgggt ggagtattta cggtaaactg
     8341 cccacttggc agtacatcaa gtgtatcata tgccaagtac gccccctatt gacgtcaatg
     8401 acggtaaatg gcccgcctgg cattatgccc agtacatgac cttatgggac tttcctactt
     8461 ggcagtacat ctacgtatta gtcatcgcta ttaccatggt cgaggtgagc cccacgttct
     8521 gcttcactct ccccatctcc cccccctccc cacccccaat tttgtattta tttatttttt
     8581 aattattttg tgcagcgatg ggggcggggg gggggggggg gcgcgccc
//
