LOCUS       sequence-208169-        5677 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  To test the effect of sequence on TDP43 droplet properties.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5677)
  TITLE     Rajat Rohatgi TDP43 plasmids
REFERENCE   2  (bases 1 to 5677)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pHBS932 TDP43 RRM-GFP Phi-S
FEATURES             Location/Qualifiers
     source          1..5677
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        21..39
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     21..38
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     protein_bind    94..118
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     regulatory      137..146
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     protein_bind    complement(1646..1670)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     polyA_signal    1715..1849
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(1739..1758)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     1793..1812
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     complement(1962..1982)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(1962..1980)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(2001..2017)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2001..2017)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(2014..2036)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    2025..2041
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(2049..2055,2056..2073,2074..2079))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2094..2115
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(2232..2249)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(2403..2991)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2483..2502)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(3162..3953,3954..4022))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     3785..3804
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(4023..4127)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(4153..4608)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4290..4311)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     4502..4521
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        4679..5663
                     /label=CMV IE94 promoter
                     /note="enhancer/promoter region of simian cytomegalovirus 
                     major immediate early transcription unit IE94"
ORIGIN
        1 gggacgtcgg agcaagcttg atttaggtga cactatagaa tacaagctac ttgttctttt
       61 tgcaggatcc catcgattcg aattcaagga tcaacaagtt tgtacaaaaa agcaggctgg
      121 cgccggtacc accggtgcca ccatgggatc catgtctgaa tatattcggg taaccgaaga
      181 tgagaacgat gagcccattg aaataccatc ggaagacgat gggacggtgc tgctctccac
      241 ggttacagcc cagtttccag gggcgtgtgg gcttcgctac aggaatccag tgtctcagtg
      301 tatgagaggt gtccggctgg tagaaggaat tctgcatgcc ccagatgctg gctggggaaa
      361 tctggtgtat gttgtcaact atccaaaaga taacaaaaga aaaatggatg agacagatgc
      421 ttcatcagca gtgaaagtga aaagagcagt ccagaaaaca tccagtaaag gagaggaact
      481 atttacaggt actgtgccta tcaaagtcga gctggacggc gacgtaaacg gccacaagtt
      541 cagcgtgcgt ggcgagggcg agggcgatgc caccgagggc aagctgaccc tgaagttcat
      601 ctgcaccacc ggcaagctgc cggtgccgtg gccgaccctc gtgaccaccc tgacctacgg
      661 cgtgcagtgc ttcagccgct acccggacca catgaagcgt cacgacttct tcaagtccgc
      721 catgccggaa ggctacgtcc aggagcgcac catcgagttc aaggacgacg gcacctacaa
      781 gacccgcgcg gaggtgaagt tcgagggcga caccctggtg aaccgcatcg agctgaaggg
      841 caacgacttc aaggaggacg gcaacatcct gggtcacaag ctggagtaca accacaacag
      901 ccacaacgtc cgtatcgaag ccgacaagca gaagaacggc atcaaggcta acttcaagat
      961 ccgccacaac gttgaggacg gcagccagca ggaagccgac cacaaacagc agaacacccc
     1021 gatcggcgac ggcccggtgc gcctgccgga caaccactat ctgagcaccc agaccacgct
     1081 gagcaaagac ccgaacgaga agcgcgatca catggtcctg aaggagttcg tgacggcagc
     1141 cggtatcact aaaggtgaag atgaaagtgg atccaatgcc gaacctaaac acaattccaa
     1201 tcggcagctc gaacgttctg gccggtccgg tgggaatcct ggtggtagtg gtaatcaagg
     1261 aggcagtgga aattcacgcg ggggtggtgc tggcagtggc aataatcaag ggagtaacag
     1321 cggaggcggg agtaattcgg gcgcttcttc gatcaatcca gcgatgatgg cggctgccca
     1381 ggcggccctt caaagttcct gggggatgat gggtatgctt gcatcacaac agaaccaaag
     1441 cgggcctagc ggtaataatc agaaccaggg caatagccag agagagccta accaggctag
     1501 tggcagtggg aacaactcgt ccagcggtag caactcggga gcagcctctg gttctggcag
     1561 tgcttctaac gctggtagcg gatcaggcag taatggcggc tccggttctt cttctgattc
     1621 caaatcttcc ggttcaggta gttagaccca gctttcttgt acaaagtggt tgatcctctc
     1681 gagcctctag aactatagtg agtcgtatta cgtagatcca gacatgataa gatacattga
     1741 tgagtttgga caaaccacaa ctagaatgca gtgaaaaaaa tgctttattt gtgaaatttg
     1801 tgatgctatt gctttatttg taaccattat aagctgcaat aaacaagtta acaacaacaa
     1861 ttgcattcat tttatgtttc aggttcaggg ggaggtgtgg gaggtttttt aattcgcggc
     1921 cgcggcgcca atgcattggg cccggtaccc agcttttgtt ccctttagtg agggttaatt
     1981 gcgcgcttgg cgtaatcatg gtcatagctg tttcctgtgt gaaattgtta tccgctcaca
     2041 attccacaca acatacgagc cgggagcata aagtgtaaag cctggggtgc ctaatgagtg
     2101 agctaactca cattaattgc gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg
     2161 tgccagctgc attaatgaat cggccaacgc gcggggagag gcggtttgcg tattgggcgc
     2221 tcttccgctt cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta
     2281 tcagctcact caaaggcggt aatacggtta tccacagaat caggggataa cgcaggaaag
     2341 aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg
     2401 tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg
     2461 tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg
     2521 cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga
     2581 agcgtggcgc tttctcatag ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc
     2641 tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt
     2701 aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact
     2761 ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg
     2821 cctaactacg gctacactag aagaacagta tttggtatct gcgctctgct gaagccagtt
     2881 accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt
     2941 ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaagatcct
     3001 ttgatctttt ctacggggtc tgacgctcag tggaacgaaa actcacgtta agggattttg
     3061 gtcatgagat tatcaaaaag gatcttcacc tagatccttt taaattaaaa atgaagtttt
     3121 aaatcaatct aaagtatata tgagtaaact tggtctgaca gttaccaatg cttaatcagt
     3181 gaggcaccta tctcagcgat ctgtctattt cgttcatcca tagttgcctg actccccgtc
     3241 gtgtagataa ctacgatacg ggagggctta ccatctggcc ccagtgctgc aatgataccg
     3301 cgagacccac gctcaccggc tccagattta tcagcaataa accagccagc cggaagggcc
     3361 gagcgcagaa gtggtcctgc aactttatcc gcctccatcc agtctattaa ttgttgccgg
     3421 gaagctagag taagtagttc gccagttaat agtttgcgca acgttgttgc cattgctaca
     3481 ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat tcagctccgg ttcccaacga
     3541 tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag cggttagctc cttcggtcct
     3601 ccgatcgttg tcagaagtaa gttggccgca gtgttatcac tcatggttat ggcagcactg
     3661 cataattctc ttactgtcat gccatccgta agatgctttt ctgtgactgg tgagtactca
     3721 accaagtcat tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata
     3781 cgggataata ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg aaaacgttct
     3841 tcggggcgaa aactctcaag gatcttaccg ctgttgagat ccagttcgat gtaacccact
     3901 cgtgcaccca actgatcttc agcatctttt actttcacca gcgtttctgg gtgagcaaaa
     3961 acaggaaggc aaaatgccgc aaaaaaggga ataagggcga cacggaaatg ttgaatactc
     4021 atactcttcc tttttcaata ttattgaagc atttatcagg gttattgtct catgagcgga
     4081 tacatatttg aatgtattta gaaaaataaa caaatagggg ttccgcgcac atttccccga
     4141 aaagtgccac ctaaattgta agcgttaata ttttgttaaa attcgcgtta aatttttgtt
     4201 aaatcagctc attttttaac caataggccg aaatcggcaa aatcccttat aaatcaaaag
     4261 aatagaccga gatagggttg agtgttgttc cagtttggaa caagagtcca ctattaaaga
     4321 acgtggactc caacgtcaaa gggcgaaaaa ccgtctatca gggcgatggc ccactacgtg
     4381 aaccatcacc ctaatcaagt tttttggggt cgaggtgccg taaagcacta aatcggaacc
     4441 ctaaagggag cccccgattt agagcttgac ggggaaagcc ggcgaacgtg gcgagaaagg
     4501 aagggaagaa agcgaaagga gcgggcgcta gggcgctggc aagtgtagcg gtcacgctgc
     4561 gcgtaaccac cacacccgcc gcgcttaatg cgccgctaca gggcgcgtcc cattcgccat
     4621 tcaggctgcg caactgttgg gaagggcgat cggtgcgggc ctcttcgcta ttacgccagt
     4681 cgaccgccaa ttcaatatgg cgtatatgga ctcatgccaa ttcaatatgg tggatctgga
     4741 cctgtgccaa ttcaatatgg cgtatatgga ctcgtgccaa ttcaatatgg tggatctgga
     4801 ccccagccaa ttcaatatgg cggacttggc accatgccaa ttcaatatgg cggacctggc
     4861 actgtgccaa ctggggaggg gtctacttgg cacggtgcca agtttgagga ggggtcttgg
     4921 ccctgtgcca agtccgccat attgaattgg catggtgcca ataatggcgg ccatattggc
     4981 tatatgccag gatcaatata taggcaatat ccaatatggc cctatgccaa tatggctatt
     5041 ggccaggttc aatactatgt attggcccta tgccatatag tattccatat atgggttttc
     5101 ctattgacgt agatagcccc tcccaatggg cggtcccata taccatatat ggggcttcct
     5161 aataccgccc atagccactc ccccattgac gtcaatggtc tctatatatg gtctttccta
     5221 ttgacgtcat atgggcggtc ctattgacgt atatggcgcc tcccccattg acgtcaatta
     5281 cggtaaatgg cccgcctggc tcaatgccca ttgacgtcaa taggaccacc caccattgac
     5341 gtcaatggga tggctcattg cccattcata tccgttctca cgccccctat tgacgtcaat
     5401 gacggtaaat ggcccacttg gcagtacatc aatatctatt aatagtaact tggcaagtac
     5461 attactattg gaagtacgcc agggtacatt ggcagtactc ccattgacgt caatggcggt
     5521 aaatggcccg cgatggctgc caagtacatc cccattgacg tcaatgggga ggggcaatga
     5581 cgcaaatggg cgttccattg acgtaaatgg gcggtaggcg tgcctaatgg gaggtctata
     5641 taagcaatgc tcgtttaggg aaccgccatt ctgcctg
//
