LOCUS       sequence-208183-       17523 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of a fungal biosynthetic gene cluster.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 17523)
  AUTHORS   Harvey CJB, Tang M, Schlecht U, Horecka J, Fischer CR, Lin HC, Li J,
            Naughton B, Cherry J, Miranda M, Li YF, Chu AM, Hennessy JR, Vandova
            GA, Inglis D, Aiyar RS, Steinmetz LM, Davis RW, Medema MH, Sattely 
            E, Khosla C, St Onge RP, Tang Y, Hillenmeyer ME
  TITLE     HEx: A heterologous expression platform for the discovery of fungal 
            natural products.
  JOURNAL   Sci Adv. 2018 Apr 11;4(4):eaar5459. doi: 10.1126/sciadv.aar5459. 
            eCollection 2018 Apr.
  PUBMED    29651464
REFERENCE   2  (bases 1 to 17523)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Adv."; 
            date: "2018-04-11"; volume: "4(4):eaar5459. doi"; pages: " 
            10.1126/sciadv.aar5459. eCollection 2018 Apr"
COMMENT     Sequence Label: pCHKU27.2-2.2
FEATURES             Location/Qualifiers
     source          1..17523
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      1272..1571
                     /label=ADH2 terminator
                     /note="transcription terminator for the S. cerevisiae 
                     alcohol dehydrogenase 2 (ADH2) gene"
     promoter        4198..4418
                     /gene="S. cerevisiae URA3"
                     /label=URA3 promoter
     CDS             4419..5222
                     /codon_start=1
                     /gene="S. cerevisiae URA3"
                     /product="orotidine-5'-phosphate decarboxylase, required 
                     for uracil biosynthesis"
                     /label=URA3
                     /note="yeast auxotrophic marker, counterselectable with 
                     5-fluoroorotic acid (5-FOA)"
                     /translation="MSKATYKERAATHPSPVAAKLFNIMHEKQTNLCASLDVRTTKELL
                     ELVEALGPKICLLKTHVDILTDFSMEGTVKPLKALSAKYNFLLFEDRKFADIGNTVKLQ
                     YSAGVYRIAEWADITNAHGVVGPGIVSGLKQAAEEVTKEPRGLLMLAELSCKGSLSTGE
                     YTKGTVDIAKSDKDFVIGFIAQRDMGGRDEGYDWLIMTPGVGLDDKGDALGQQYRTVDD
                     VVSTGSDIIIVGRGLFAKGRDAKVEGERYRKAGWEAYLRRCGQQN"
     primer_bind     complement(13670..13690)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(13670..13688)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     rep_origin      complement(14027..14615)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(14107..14126)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(14786..15577,15578..15646))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     15409..15428
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(15647..15751)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      15778..17120
                     /label=2u ori
                     /note="yeast 2u plasmid origin of replication"
     protein_bind    complement(16741..16788)
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     primer_bind     17161..17179
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(17498..17514)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(17498..17514)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
ORIGIN
        1 tatctaaaaa ttgccttatg atccgtctct ccggttacag cctgtgtaac tgattaatcc
       61 tgcctttcta atcaccattc taatgtttta attaagggat tttgtcttca ttaacggctt
      121 tcgctcataa aaatgttatg acgttttgcc cgcaggcggg aaaccatcca cttcacgaga
      181 ctgatctcct ctgccggaac accgggcatc tccaacttat aagttggaga aataagagaa
      241 tttcagattg agagaatgaa aaaaaaaaaa aaaaaaaagg cagaggagag catagaaatg
      301 gggttcactt tttggtaaag ctatagcatg cctatcacat ataaatagag tgccagtagc
      361 gacttttttc acactcgaaa tactcttact actgctctct tgttgttttt atcacttctt
      421 gtttcttctt ggtaaataga atatcaagct acaaaaagca tacaatcaac tatcaactat
      481 taactatatc gtaatacaca atgacaatcc tctgtctaca tggcggcttc ggcagcgccc
      541 aaaacttcca agtgcaactc catcccctag ttagtaccta ccaatcatta taccccgata
      601 ccaccttcca ctacattaat ggcggtcatc ccgccacgcc tccaccagga agcgaggatt
      661 acttcggccc accgcctcat taccgattca tcgaatacga tgggattggg cggtccgacg
      721 atgtgatgga gcggattcgt cagctcccga ggggagccac ggccgaagat accatgcggg
      781 tgttgaccca cgaacatgag atgatgtctg ccgattgtgt gagagaggcg ttgcagagac
      841 tgttggggat attggatgaa catccggaaa ttgacggagt cttgggcttc tcggagggcg
      901 ctacagtcgc tgctactttg ttgcttgaag aggagcgact tgttcaggag gagggtagga
      961 agagacagct taagtacggg gtcttcatag caggctggcc tccccttcgt attgacggcg
     1021 atcgagtaac aggctgtctg gcggatgaga cagaggatat gatcgaagtg ccgacgtgtc
     1081 atatcatcgg ggcgaatgac ccctacgtgg atggcaccat ggcgctgtat ggggtttgtg
     1141 atcccgatac ggccatcatg tttgatcatg gcaagggcca taccattccg cgggacgtgg
     1201 cgacgatgaa ggaggcggca gaggtgattg agcagaccag gagacggggg attgctgccg
     1261 gggatgcgta ggcggatctc ttatgtcttt acgatttata gttttcatta tcaagtatgc
     1321 ctatattagt atatagcatc tttagatgac agtgttcgaa gtttcacgaa taaaagataa
     1381 tattctactt tttgctccca ccgcgtttgc tagcacgagt gaacaccatc cctcgcctgt
     1441 gagttgtacc cattcctcta aactgtagac atggtagctt cagcagtgtt cgttatgtac
     1501 ggcatcctcc aacaaacagt cggttatagt ttgtcctgct cctctgaatc gtctccctcg
     1561 atatttctca ttttccttcg catgccagca ttgaaatgat cgaagttcaa tgatgaaacg
     1621 gtaattcttc tgtcatttac tcatctcatc tcatcaagtt atataattct atacggatgt
     1681 aatttttcac ttttcgtctt gacgtccacc ctataatttc aattattgaa ccctcacgat
     1741 ccagttctcc agtgacacag cctttatctg gtcaaacctt tctttctaat cacctatgct
     1801 gatgcttaat taagggattt ttgtctccat caacggcatg cgcccaaaaa tgacgttttt
     1861 tttaacccat agacacgaaa ctacccattt tccaccggcc tgacctacca ccggaacaac
     1921 ggccatctcc aacttgcaag ttggggaaat taagagcatc gcaggtttaa tggaagaaaa
     1981 aaaaaaggta cagcacagcg caaatggagt tagttccctt atgtcacaca ctcacacaca
     2041 gtcggtcaga tcaagcatac tgggtgcgta taaatagagt ggccattgcc accctgttta
     2101 tctcaaaatc tgtcttgtta gtggtcttct ccctttttca ggttacaatt ctcttgtttc
     2161 tacttagtat ataagtatat caagctatat taagcatact atcaactgtc aactctatcc
     2221 tcaaaataca atacaaaatg cagctctcat tgttggctct tcttggagca ttggcgctgc
     2281 ccagcgctgc tgttccccat gcgcgcgatg agggacaatg caagcagacc actgttgcta
     2341 ttcttggtgg aggaatggct ggtattgctg ctgctcaaac gctgcacaat gcctccatgg
     2401 atgacttcat gatcctcgaa taccgcgaca ccatcggcgg tcgcgcctgg cataagccct
     2461 tcggtcagga caaagatggg aacccataca tcattgagat gggttgtaac tgggtccaag
     2521 gactcggcac tcctgggggt ccgcagaacc ccgtctggac actggcccaa gtctacaacc
     2581 tctccaccat ctactccaac tacagcaacg tgtcgaccta caaccagcac ggctacaaag
     2641 actactccca cctgatcgac acctgggatg acatctacga caccgccgcc gcccaagccg
     2701 gcgtcatgct cctcgacaac ctgcaggacc agacagccca gaccggactg gccctcgccg
     2761 gctggcgccc caaggtcgac gacatggaag cacaagccgt tgactggtgg tcatgggact
     2821 tcgaagacgc ctacacgccc ctcgaaagtt cctttatctt cggcgtcgca ggccaaaacc
     2881 tcaccgtgaa cggcttcagc gacgaagaca actttgtcat cgaccaacgc ggctacagcc
     2941 acatcatcca cggcatggcg tcgaccttcc tcaagcccaa cgacactcgc ctgctcctca
     3001 acaaccacat caccaacatc agctactccg actccggtgt gaccgtccac agttccgacg
     3061 gctcctgcgt gcgcgcctcc tacgccatct gcaccttcag tctgggcgta ttgcaacacg
     3121 acgccgtcac cttcaccccg tccctgcccg aatggaagaa agaagccatc gaaggcttca
     3181 caatggccac ctacaccaag atcttcctcc agttcaacga gaccttctgg cccgaagaca
     3241 cccaatactt cctctacgcg gacccctaca tgcgcggcta ctaccccgtc ttccagtccc
     3301 tcagcaccga gggcttcttc ccgggctcca acatcatctt cgtcaccgtg acggagcaat
     3361 tcgcctggcg cgccgaacgc cagtccgacg agaagaccaa agcagaggtg atggaagtcc
     3421 tgcgcaagat gttcccggag aaggatatcc ctgacccgat tgcgttcatg tacccgcggt
     3481 ggaccctgga gccatgggct tacggcagtt actcgaactg gccgcccagc acgacgttgg
     3541 agatgcatga gaacctgcgt gccaatgcgg ggagactgtg gtttgccggt gaggcaacca
     3601 gtccaaccta ctttgggttc ttgcatggag cttggttcga gggccaggct gcggggcttc
     3661 atcttagcag tatcttgaac ggcacttgca aggcgaatac tacttgcaag ggcaggaagc
     3721 attacgagac gttgcacggc acgacgccat tgtcggatta cagcttcgtg gatggatgga
     3781 acggaaacag tttctatgat tttaacgatg attaaacaaa tcgctcttaa atatatacct
     3841 aaagaacatt aaagctatat tataagcaaa gatacgtaaa ttttgcttat attattatac
     3901 acatatcata tttctatatt tttaagattt ggttatataa tgtacgtaat gcaaaggaaa
     3961 taaattttat acattattga acagcgtcca agtaactaca ttatgtgcac taatagttta
     4021 gcgtcgtgaa gactttattg tgtcgcgaaa agtaaaaatt ttaaaaatta gagcaccttg
     4081 aacttgcgaa aaaggttctc atcaactgtt taaaaggagg atatcaggtc ctatttctga
     4141 caaacaatat acaaatttag tttcaaagat gaatcagtgc gcgaaggaca taactcattc
     4201 aattcaattc atcatttttt ttttattctt ttttttgatt tcggtttctt tgaaattttt
     4261 ttgattcggt aatctccgaa cagaaggaag aacgaaggaa ggagcacaga cttagattgg
     4321 tatatatacg catatgtagt gttgaagaaa catgaaattg cccagtattc ttaacccaac
     4381 tgcacagaac aaaaacctgc aggaaacgaa gataaatcat gtcgaaagct acatataagg
     4441 aacgtgctgc tactcatcct agtcctgttg ctgccaagct atttaatatc atgcacgaaa
     4501 agcaaacaaa cttgtgtgct tcattggatg ttcgtaccac caaggaatta ctggagttag
     4561 ttgaagcatt aggtcccaaa atttgtttac taaaaacaca tgtggatatc ttgactgatt
     4621 tttccatgga gggcacagtt aagccgctaa aggcattatc cgccaagtac aattttttac
     4681 tcttcgaaga cagaaaattt gctgacattg gtaatacagt caaattgcag tactctgcgg
     4741 gtgtatacag aatagcagaa tgggcagaca ttacgaatgc acacggtgtg gtgggcccag
     4801 gtattgttag cggtttgaag caggcggcag aagaagtaac aaaggaacct agaggccttt
     4861 tgatgttagc agaattgtca tgcaagggct ccctatctac tggagaatat actaagggta
     4921 ctgttgacat tgcgaagagc gacaaagatt ttgttatcgg ctttattgct caaagagaca
     4981 tgggtggaag agatgaaggt tacgattggt tgattatgac acccggtgtg ggtttagatg
     5041 acaagggaga cgcattgggt caacagtata gaaccgtgga tgatgtggtc tctacaggat
     5101 ctgacattat tattgttgga agaggactat ttgcaaaggg aagggatgct aaggtagagg
     5161 gtgaacgtta cagaaaagca ggctgggaag catatttgag aagatgcggc cagcaaaact
     5221 aaaaaactgt attataagta aatgcatgta tactaaactc acaaattaga gcttcaattt
     5281 aattatatca gttattaccc tatgcggtgt gaaataccgc acagatgcgt aaggagaaaa
     5341 taccgcatca ggataggaaa aaaccgagct tcctttcatc cggcgcggct gtgttctaca
     5401 tatcactgaa gctccgggta ttttaagtta tacaagggaa agatgccggc tagactagca
     5461 agttttaggc tgcttaacat tatggatagg cggataaagg gcccaaacag gattgtaaag
     5521 cttagacgct tctggttgga caatggtacg tttgtgtatt aagtaaggct tggctgggga
     5581 tagcaacatt gggcagagta tagaagacca caaaaaaaag gtatataagg gcagagaagt
     5641 ctttgtaatg tgtgtaactt ctcttccatg tgtaatcagt atttctactt acttcttaaa
     5701 tatacagaag taagacagat aaccaacagc ctttcccaga tatacatata tatctttatt
     5761 tcagcttaaa caataattat atttgtttaa ctcaaaaata aaaaaaaaaa accaaactca
     5821 cgcaactaat tattccataa taaaataaca acatggctac cacccccgga caacgtctct
     5881 tccctgtggt cgtcgaccac ctcgcccaga ccaacccagc tcagcgcatc ggactcatcc
     5941 ccaagggtgc tgatgtctct gatggctatg atactgtgac cgtccagcgt ctggcccgcg
     6001 cggtgaatgt ctttgcttgg tggattgaga agcagatcgg acgcgcccag aagccagaga
     6061 caattgctta catggctagc aatgatgtgc gatacttgat tttcctcttg gccgctcata
     6121 agacgggata caagcccctc ctcccctcta ctcgtctttc cgacgacgcc taccagcaca
     6181 tcctcaacgc caccgactgc cacgccttct tctacacccc cgaaaagcag cgccgcgtgt
     6241 ctgaaattca ggccttccgt ccggatactg ccttccatga ggtgccctct atcgcagagc
     6301 ttttcagcac cactgctcag ccctacccct acaccaagac ctaccaggaa gcggaggacg
     6361 atgtcgccat catcatccac agctccggca caacaggcat gcctaaaccc gtccccctga
     6421 cgcacggctt cctcgccacc ctcgactacg gcgcatacat ttcgcgtccc caatcccgcc
     6481 aatccaccat gttcaacgac ctccagcccg acaccctcgt cctctccacc acccccttct
     6541 tccaccttat gggtctgctc gccttcgccg aatccctctt ccaccacatc ccctttgtcg
     6601 ttctccccga caagcctctc tccgccgacc tcctcacctc cgtcatcacc gccaccaagc
     6661 ccgcggccgc catgctgccc ccttcccttc tcgaagacat gtcccacgtc ccagctgccc
     6721 gcgccgcact cagcaccctc gaaaatgtct acttcgctgg cgcccctctc tccccggaag
     6781 tcggcgatga tctgtcccag ctgaccaaga tcatcactgt catcggatcc agcgaaatgg
     6841 gcctgatctc ctcgttcgtg ccccaaggca caaatgtgtg gggctacttc gactggaacc
     6901 ccgcctacgg agtagacatg cgacatgtca gcgacgatat gtatgagctt atcatccccc
     6961 gccgcgcaaa cagtcgccga attcatggca tcttccacac cttcccagac cgctccgagt
     7021 accgctccaa cgacctcttc acccgacacc ccgagcaccc tcacctgtgg aaataccacg
     7081 gccgacttga cgacgtgatc gttctcagca acggcgagaa actgaacccc gtcaccctgg
     7141 agaagatcgt cgaggggcac ccgcgcgtgc agcgggctct gctcatcggc caagcccgct
     7201 tcgacacggc cctcatcatc gaacccaaca gggctctcga gggcgagaac atcgacgaaa
     7261 aagcctttat cgagagcatt tggcccaccg tggagcgcgc gaacgaagcc gtccccaaat
     7321 acggccgcat cgcaaagacc aggatccgtc tctctgaccc caagaagccc ttccaactga
     7381 cccccaaggg caccacgaag cgccacgccg tgaacaagga ctacaaagac gagatcgacg
     7441 ccatctacgc agcagccgag gaagaatcag ccgccgtcga gctccctacc accctcacca
     7501 ccgacaacct ccgcactttc gtccgctccc tcatcgccaa cctcctcgac cgcactgacc
     7561 tccgcgacga cgaggacctc tacgccgccg gcctggactc gctgcaaacc atccagctcg
     7621 ccaaaaccct taacaaggcc gtcgtcgccg ccggctaccc cgccggtcac gacatctccc
     7681 agcagcaaat ctatgccaac ccctccgtat cccagctcgc tgcgtaccta cacgacctag
     7741 tgaccggcaa cctgcgcaca acgaccatct cccgcgccga ccgcatcgcc gccaccatcg
     7801 acaaatacac caccaagctc ccgcgccgca ccacgcccgc cgtccctgcc ccggccaact
     7861 ccaccgtgat cctcaccggc tcgaccgggt cgctgggaac ctacctccta cacagcctgc
     7921 tcaccgaccc aaagatcgca aaagtctact gcttcaaccg gtccgatgcg gccgagcgcc
     7981 aacagcgcag cttcgccgac aagggtctgg atactgcgct gctcaacccc agccgggtgg
     8041 aattcctcac tgtgtcattt ggcgcgcccc gcttcggcct gagtgagaca aagtacgccg
     8101 atctcctgga gactgtggac ttcattatcc acaacgcatg gaaagtcaac ttcaatcacc
     8161 cgctggagtc gttcgaggac ccgcatatca agggcgtggt ggagtttatt tccttcagtg
     8221 tgaggagcaa gtacaacgca cacttgtcgt tcgtgtccag tgtgagtacc atcggtggat
     8281 ggacggagaa gttgggcccc gtggtgccgg agacgccggt ggagagtgtc gatgcggtgc
     8341 tggtgcaggg gtacggcgag tcgaagttcg tcggggagag gttgtgtctg gctgcgtcgg
     8401 aggggatggg tgtgcccacg acggtgttta gggttgggca gatttccggg ccgacgactg
     8461 agaagggcat gtggaatttg gatgagtggg tgcctacgtt ggtgaagacc agtaaggcgg
     8521 ttggaaaggt gccggcggat ttgggtgggt atgaggttga ttgggtgcct gttgataccc
     8581 tctccaccat caccaccgaa ctcatgcaca cccgtcgcca aacccaatcc gctactccac
     8641 acgccgtcta ccaccttgtc aacccctgca agactccctg ggcgaccctc gtccccgccg
     8701 ttcaggccaa ataccccggt atgcagacgg tgccgctaga ccagtggctg gacgagctgg
     8761 aggcgatcaa gtcaccgtcg gaaaccgagg tgcgcgagaa gcccgcgctg aaactgcttg
     8821 atttctaccg cggactcgcg ggcgaggtgc tctcggccag tattagtgtg gagcagacgc
     8881 gcgggggcag caagaccatg gagggattgg gggctgtgac aggccagttg atggggaatt
     8941 ggttggggca gtgggatttc tgaagtgctt ttaactaaga attattagtc ttttctgctt
     9001 attttttcat catagtttag aacactttat attaacgaat agtttatgaa tctatttagg
     9061 tttaaaaatt gatacagttt tataagttac tttttcaaag actcgtgctg tctattgcat
     9121 aatgcactgg aaggggaaaa aaaaggtgca cacgcgtggc tttttcttga atttgcagtt
     9181 tgaaaaataa ctacatggat gataagaaaa catggagtac agtcactttg agaaccttca
     9241 atcagctggt aacgtcttcg ttaattggat actcaaaaaa gatggatagc atgaatcaca
     9301 agatggaagg aaatgcgggc cacgaccaca gtgatatgca tatgggagat ggagatgata
     9361 cctccattgg gccgatgaag ttagtcgacg gatagaagcg gttgtcccct ttcccggcga
     9421 gccggcagtc gggccgaggt tcggataaat tttgtattgt gttttgattc tgtcatgagt
     9481 attacttatg ttctctttag gtaaccccag gttaatcaat cacagtttca taccggctag
     9541 tattcaaatt atgacttttc ttctgcagtg tcagccttac gacgattatc tatgagcttt
     9601 gaatatagtt tgccgtgatt cgtatcttta attggataat aaaatgcgaa ggatcgatga
     9661 cccttattat tatttttcta cactggctac cgatttaact catcttcttg aaagtatata
     9721 agtaacagta aaatataccg tacttctgct aatgttattt gtcccttatt tttcttttct
     9781 tgtcttatgc tatagtacct aagaataacg actattgttt tgaactaaac aaagtagtaa
     9841 aagcacataa aagaattaag aaaatgcggt cactgcaggt tttcgccctg gcaggcgtcc
     9901 ttggcgcgtc tgcccttgat gccgactcta tcctgagcaa atactttggc aacgatgctc
     9961 cttggtatag cgatcgcatt cccctcttcg agacagatct ctccgacatt caagatgtct
    10021 actattaccg ctggagcatt ttccgggctc accagcgtga ccttggctcc tacggctata
    10081 tctccaccga gttcctcaac gacgtcagct ggcaggaaga accttatgcc ctgctgattg
    10141 atgcctccaa cttccatctg cgcgaaggcc gctggtgccg cgaccgtcgc ttcaccagcg
    10201 actactcgtc ttttctcttc ggcccccatg gcgcccccta ccaattctcc gaatccatgg
    10261 ccgacggcgt ctggcaggcc tacctggtca acggcgtcgc tgacgatgcc actcccctgc
    10321 tgtcctccat gcagaccgtc tacgccggct ggaactccac ctctctcggt gtcggtggct
    10381 acgacacctc caagggcctc tactggatcc agcctctgac tgatgcgacc gaatacacca
    10441 ttgccagcat cgacgcctcc ggaggcgaag acggcttcac cggcggatat gccttccgtc
    10501 ccagcatcaa cagctaccaa tatgctaatg ccctggctat tgcccacatc gccaacctca
    10561 ccggcgacac taccgtccgc gagcaataca cccagctcgc aagcgccctc aagacccgtc
    10621 tccaggacga cctctggaac agcaccttcg agcacttcat cgaccggtac aaggaagaca
    10681 acgactacgt gacctactgg gacttcatcc gcggccgcga acttgtcggc tacgtccctt
    10741 ggacacatga cctcccggac gacaacacca cctacgccaa cgcatggact cacatcctca
    10801 actcctcaca gctagcaggc gagcacggcc ttcgcaccaa cgaacccagc taccaatact
    10861 acatggtcca gtaccgctat gacggctcca acccggagtg ccagtggaac ggccccgcct
    10921 ggccctatca aaccacgcaa gtcctcaccg gcctcgccaa cctcctggac cactacccca
    10981 aaaccgccgc aacaggcgtc atcaccacca cagactacac caacatcctc ctccagtacg
    11041 ccaagctaca ctacaacccc gcccgcggcg gtgtccttga catcgaagag gactactact
    11101 ctgacacggg cagccctatc gtcggcctgc cccgctcccc gcactacttc cactcgggct
    11161 tcgtcgacct cattctctcg ggctacgtgg gcatccgacc tcgcgcggac aatgtcctgg
    11221 aagttaaccc ccaagcggac gcttcggcag tgagctactt ccgctccgag cgcatcatct
    11281 accacggcca cgaggtcgcc gtgcaatggg acgccacagg cagtcgatac ggcaccgtgg
    11341 gccttctcgt cgaagtcgac ggcgaaaccg tggccaccag caagaccctc acccgtctca
    11401 cggttgagtt aagctccgct tccgccccgg cagtctcccg tcccatcgcc gtctcggtgc
    11461 aactcgccgc tgatagcgag tatccgcggg gtagtgtatc tgtggcagac gccgacacgg
    11521 acgccgtgca ctcagccatc gacggccggg tctggttcta ttggatcgag gacgtagcca
    11581 atgggtggga tagtcccgtt gggaatggga cggaggtgtg gtaccaggtg gaatttgaga
    11641 atgcgaccag cacagcaagt gcggagattg cgttctttgc gaatagtacg caggggtatg
    11701 atgtgccggg gagctatcgg gtggaaacga gcgattcgac gaccggggag tggaaggagg
    11761 tgagcaatgg gaattatagc tcggcggtgg cgaatggaat aacgacggtg agctgggatg
    11821 aggtgacgac ggaggcgttg aggttggtgt tcacgcctaa gacgggggag atggtgcggt
    11881 tggtggagtg gaaggtgttt gggtagattt aactccttaa gttactttaa tgatttagtt
    11941 tttattatta ataattcatg ctcatgacat ctcatataca cgtttataaa acttaaatag
    12001 attgaaaatg tattaaagat tcctcaggga ttcgattttt ttggaagttt ttgttttttt
    12061 ttccttgaga tgctgtagta tttgggaaca attatacaat cgaaagatat atgcttacat
    12121 tcgaccgttt tagccgtgat cattatccta tagtaacata acctgaagca taactgacac
    12181 tactatcatc aatacttgtc acatgagaac tctgtgaata attaggccac tgaaatttga
    12241 tgcctgaagg accggcatca cggattttcg ataaagcact tagtatcaca ctaattggct
    12301 tttcgctagt cttatctaaa aattgccttt atagtccgtc tctccagtca cggcctgtgt
    12361 aactgattaa tcctgccttt ctaatcacca ttctactgtt taattaaggg attttgtctt
    12421 catcaacggc ttccgcccaa aaaaaagtat gacgttttgc ccgcaggcgt gaagctgccc
    12481 atcttcacgg gcctgacctc ctctgccgga acaccggcca tctccaactc ataaattgga
    12541 gaaataagag aatttcagat tttcagagga tgaaaaaaaa aaggtagaga gcataaaaat
    12601 ggggttcact ttttggcaaa gttacagtat gcttattaca tataaataga gtgccgataa
    12661 tggctttttt tcatcttcga aatacgcttg ctactgctct tccagcgttt ttattacttc
    12721 tttcttgttt ctccttagta tataaaatat caagctacaa caagcataca atcaactgtc
    12781 aactgtcaat tatattataa tacactatgc atctttctac attgctcctc cccctcctcc
    12841 ccaccgccgc cctctccgca atctgctacc ccgagacagg cggctcgaac tgcgcctcgc
    12901 tcctcagcat caaagaattc tactcccttc aatactgcac ctaccggtgg aacgtgcttt
    12961 acggcgactg ggaccacttc gtcaacaacg cgaccagccc gacgaaggtg cacgccagcg
    13021 tgggtaagac gggcgtgttt gatagcttcg aggactgtct taatgggttt gaggatgtcg
    13081 tggagacatg tcatggtgtt agtcagggtg gggttatgac caatgggaat tgaggagatt
    13141 gataagactt ttctagttgc atatctttta tatttaaatc ttatctatta gttaattttt
    13201 tgtaatttat ccttatatat agtctggtta ttctaaaata tcatttcagt atctaaaaat
    13261 tcccctcttt tttcagttat atcttaacag gcgacagtcc aaatgttgat ttatcccagt
    13321 ccgattcatc agggttgtga agcattttgt caatggtcga aatcacatca gtaatagtgc
    13381 ctcttacttg cctcatagaa tttctttctc ttaacgtcac cgtttggtct tttatagttt
    13441 cgaaatctat ggtgatacca aatggtgttc ccaattcatc gttacgggcg tattttttac
    13501 caattgaagt attggaatcg tcaattttaa agtatatctc tcttttacgt aaagcctgcg
    13561 agatcctctt aagtatagcg gggaagccat cgttattcga tattgtcgta acaaatactt
    13621 tgatcggcgc tatgcggccg ccaccgcggt ggagctccag cttttgttcc ctttagtgag
    13681 ggttaattgc gcgcttggcg agactcaaac aagatatatt ttgcccgcct tacagacgaa
    13741 actagttgga ggttatggag catactatca cgtgggcggc cactggtgag ttactacacc
    13801 ccaggggcaa cgttgatgct cctaaaaaac tctggctgga cgcaagccgt aacacccgtg
    13861 tcacttcata atcgtttgca attcagggct tgatctacac attaccctgt tatccctatt
    13921 aattaatacg gttatccaca gaatcagggg ataacgcagg aaagaacatg tgagcaaaag
    13981 gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc
    14041 gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag
    14101 gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga
    14161 ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc
    14221 atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg
    14281 tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt
    14341 ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca
    14401 gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca
    14461 ctagaaggac agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag
    14521 ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca
    14581 agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg
    14641 ggtctgacgc tcagtggaac gaaaactcac gttaagggat tttggtcatg agattatcaa
    14701 aaaggatctt cacctagatc cttttaaatt aaaaatgaag ttttaaatca atctaaagta
    14761 tatatgagta aacttggtct gacagttacc aatgcttaat cagtgaggca cctatctcag
    14821 cgatctgtct atttcgttca tccatagttg cctgactccc cgtcgtgtag ataactacga
    14881 tacgggaggg cttaccatct ggccccagtg ctgcaatgat accgcgagac ccacgctcac
    14941 cggctccaga tttatcagca ataaaccagc cagccggaag ggccgagcgc agaagtggtc
    15001 ctgcaacttt atccgcctcc atccagtcta ttaattgttg ccgggaagct agagtaagta
    15061 gttcgccagt taatagtttg cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac
    15121 gctcgtcgtt tggtatggct tcattcagct ccggttccca acgatcaagg cgagttacat
    15181 gatcccccat gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc gttgtcagaa
    15241 gtaagttggc cgcagtgtta tcactcatgg ttatggcagc actgcataat tctcttactg
    15301 tcatgccatc cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag tcattctgag
    15361 aatagtgtat gcggcgaccg agttgctctt gcccggcgtc aatacgggat aataccgcgc
    15421 cacatagcag aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct
    15481 caaggatctt accgctgttg agatccagtt cgatgtaacc cactcgtgca cccaactgat
    15541 cttcagcatc ttttactttc accagcgttt ctgggtgagc aaaaacagga aggcaaaatg
    15601 ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat actcatactc ttcctttttc
    15661 aatattattg aagcatttat cagggttatt gtctcatgag cggatacata tttgaatgta
    15721 tttagaaaaa taaacaaata ggggttccgc gcacatttcc ccgaaaagtg ccacctgaac
    15781 gaagcatctg tgcttcattt tgtagaacaa aaatgcaacg cgagagcgct aatttttcaa
    15841 acaaagaatc tgagctgcat ttttacagaa cagaaatgca acgcgaaagc gctattttac
    15901 caacgaagaa tctgtgcttc atttttgtaa aacaaaaatg caacgcgaga gcgctaattt
    15961 ttcaaacaaa gaatctgagc tgcattttta cagaacagaa atgcaacgcg agagcgctat
    16021 tttaccaaca aagaatctat acttcttttt tgttctacaa aaatgcatcc cgagagcgct
    16081 atttttctaa caaagcatct tagattactt tttttctcct ttgtgcgctc tataatgcag
    16141 tctcttgata actttttgca ctgtaggtcc gttaaggtta gaagaaggct actttggtgt
    16201 ctattttctc ttccataaaa aaagcctgac tccacttccc gcgtttactg attactagcg
    16261 aagctgcggg tgcatttttt caagataaag gcatccccga ttatattcta taccgatgtg
    16321 gattgcgcat actttgtgaa cagaaagtga tagcgttgat gattcttcat tggtcagaaa
    16381 attatgaacg gtttcttcta ttttgtctct atatactacg tataggaaat gtttacattt
    16441 tcgtattgtt ttcgattcac tctatgaata gttcttacta caattttttt gtctaaagag
    16501 taatactaga gataaacata aaaaatgtag aggtcgagtt tagatgcaag ttcaaggagc
    16561 gaaaggtgga tgggtaggtt atatagggat atagcacaga gatatatagc aaagagatac
    16621 ttttgagcaa tgtttgtgga agcggtattc gcaatatttt agtagctcgt tacagtccgg
    16681 tgcgtttttg gttttttgaa agtgcgtctt cagagcgctt ttggttttca aaagcgctct
    16741 gaagttccta tactttctag agaataggaa cttcggaata ggaacttcaa agcgtttccg
    16801 aaaacgagcg cttccgaaaa tgcaacgcga gctgcgcaca tacagctcac tgttcacgtc
    16861 gcacctatat ctgcgtgttg cctgtatata tatatacatg agaagaacgg catagtgcgt
    16921 gtttatgctt aaatgcgtac ttatatgcgt ctatttatgt aggatgaaag gtagtctagt
    16981 acctcctgtg atattatccc attccatgcg gggtatcgta tgcttccttc agcactaccc
    17041 tttagctgtt ctatatgctg ccactcctca attggattag tctcatcctt caatgctatc
    17101 atttcctttg atattggatc atactaagaa accattatta tcatgacatt aacctataaa
    17161 aataggcgta tcacgaggcc ctttcgtctc gcgcgtttcg gtgatgacgg tgaaaacctc
    17221 ggcgcgccat taccctgtta tccctaatat acaacgcaaa gaagctggaa aattggcgag
    17281 ggaatcctgt ttctgtctat ccaagaatgg gcatgaggtg gcaaccgtcg tgctagcgta
    17341 cagggtgcac tttgtaacca tttgggacac cggacactcg ctgttttcga aattaccctt
    17401 taagcgcggg tattgaacca ggcttatgcc caagatcgta gcaagcattt aaatgacggt
    17461 atcgataagc ttgatatcga attcctgcag cccgggggat ccactagttc tagagttaat
    17521 taa
//
