LOCUS       sequence-208185-       13617 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of a fungal biosynthetic gene cluster.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13617)
  AUTHORS   Harvey CJB, Tang M, Schlecht U, Horecka J, Fischer CR, Lin HC, Li J,
            Naughton B, Cherry J, Miranda M, Li YF, Chu AM, Hennessy JR, Vandova
            GA, Inglis D, Aiyar RS, Steinmetz LM, Davis RW, Medema MH, Sattely 
            E, Khosla C, St Onge RP, Tang Y, Hillenmeyer ME
  TITLE     HEx: A heterologous expression platform for the discovery of fungal 
            natural products.
  JOURNAL   Sci Adv. 2018 Apr 11;4(4):eaar5459. doi: 10.1126/sciadv.aar5459. 
            eCollection 2018 Apr.
  PUBMED    29651464
REFERENCE   2  (bases 1 to 13617)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Adv."; 
            date: "2018-04-11"; volume: "4(4):eaar5459. doi"; pages: " 
            10.1126/sciadv.aar5459. eCollection 2018 Apr"
COMMENT     Sequence Label: pCHKU30.2-2.2
FEATURES             Location/Qualifiers
     source          1..13617
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      1371..1670
                     /label=ADH2 terminator
                     /note="transcription terminator for the S. cerevisiae 
                     alcohol dehydrogenase 2 (ADH2) gene"
     promoter        4303..4523
                     /gene="S. cerevisiae URA3"
                     /label=URA3 promoter
     CDS             4524..5327
                     /codon_start=1
                     /gene="S. cerevisiae URA3"
                     /product="orotidine-5'-phosphate decarboxylase, required 
                     for uracil biosynthesis"
                     /label=URA3
                     /note="yeast auxotrophic marker, counterselectable with 
                     5-fluoroorotic acid (5-FOA)"
                     /translation="MSKATYKERAATHPSPVAAKLFNIMHEKQTNLCASLDVRTTKELL
                     ELVEALGPKICLLKTHVDILTDFSMEGTVKPLKALSAKYNFLLFEDRKFADIGNTVKLQ
                     YSAGVYRIAEWADITNAHGVVGPGIVSGLKQAAEEVTKEPRGLLMLAELSCKGSLSTGE
                     YTKGTVDIAKSDKDFVIGFIAQRDMGGRDEGYDWLIMTPGVGLDDKGDALGQQYRTVDD
                     VVSTGSDIIIVGRGLFAKGRDAKVEGERYRKAGWEAYLRRCGQQN"
     regulatory      6714..6723
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(9764..9784)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(9764..9782)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     rep_origin      complement(10121..10709)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(10201..10220)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(10880..11671,11672..11740))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     11503..11522
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(11741..11845)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      11872..13214
                     /label=2u ori
                     /note="yeast 2u plasmid origin of replication"
     protein_bind    complement(12835..12882)
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     primer_bind     13255..13273
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(13592..13608)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(13592..13608)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
ORIGIN
        1 tatctaaaaa ttgccttatg atccgtctct ccggttacag cctgtgtaac tgattaatcc
       61 tgcctttcta atcaccattc taatgtttta attaagggat tttgtcttca ttaacggctt
      121 tcgctcataa aaatgttatg acgttttgcc cgcaggcggg aaaccatcca cttcacgaga
      181 ctgatctcct ctgccggaac accgggcatc tccaacttat aagttggaga aataagagaa
      241 tttcagattg agagaatgaa aaaaaaaaaa aaaaaaaagg cagaggagag catagaaatg
      301 gggttcactt tttggtaaag ctatagcatg cctatcacat ataaatagag tgccagtagc
      361 gacttttttc acactcgaaa tactcttact actgctctct tgttgttttt atcacttctt
      421 gtttcttctt ggtaaataga atatcaagct acaaaaagca tacaatcaac tatcaactat
      481 taactatatc gtaatacaca atgaagatcc tctgccttca cggtagaggc tccaacaacg
      541 agatctttcg ggcgcagacg gcagccttcc gtgcagatct cgatgacttt gagtggacct
      601 ttgtgcaagg cacggttcga cataccgaag gcaactggtc cctgtacaca gcacaattct
      661 cgcatcttcc actttacaca tactacaacc cttttgatcc tgcttctatc ttgcagactc
      721 atgacgacct tgcgcggatt atagtcgaag agggcccatt cgatggtatt ctgggctatt
      781 caggcggtgc tgcgttagca gcagagatct tggctcaggc aacctatgat gatccctttg
      841 ccatggaacg accgtttcga tttgcagtct tcatcaacgg agcatctccc ctgcgctgtt
      901 ttgctatgaa ggacgcccaa gtcatcgagg acatcgtcgt agattccgca cccatgaccg
      961 agcaggcaga gaatatgttt cttcgtccga gtgcgctgcg aaagaaggac ggagtcagcg
     1021 aacaggacca ggtcgaccat gcaaaactac tgtcgttgct caagaaactc aagggcaaga
     1081 agttggtcga tggaacaacg tttctatcca acggaacgca tggcctttgt agatgggaac
     1141 tacccgcaga cggtgcgcca ctgattgact tgcccacgct gcacatccgg agcaccgaga
     1201 gcgacgagag tgatccccac gagggtctcc atttgctacg actctgtgat cccggccaag
     1261 cacgcgagtt ccatcatggc agtgggcatg atttccccag aggtcgtaag gaaatgaagc
     1321 acattgcgag actgattcga gaggtcgctg aagctacaac aagtctataa gcggatctct
     1381 tatgtcttta cgatttatag ttttcattat caagtatgcc tatattagta tatagcatct
     1441 ttagatgaca gtgttcgaag tttcacgaat aaaagataat attctacttt ttgctcccac
     1501 cgcgtttgct agcacgagtg aacaccatcc ctcgcctgtg agttgtaccc attcctctaa
     1561 actgtagaca tggtagcttc agcagtgttc gttatgtacg gcatcctcca acaaacagtc
     1621 ggttatagtt tgtcctgctc ctctgaatcg tctccctcga tatttctcat tttccttcgc
     1681 atgccagcat tgaaatgatc gaagttcaat gatgaaacgg taattcttct gtcatttact
     1741 catctcatct catcaagtta tataattcta tacggatgta atttttcact tttcgtcttg
     1801 acgtccaccc tataatttca attattgaac cctcacgatc cagttctcca gtgacacagc
     1861 ctttatctgg tcaaaccttt ctttctaatc acctatgctg atgcttaatt aagggatttt
     1921 tgtctccatc aacggcatgc gcccaaaaat gacgtttttt ttaacccata gacacgaaac
     1981 tacccatttt ccaccggcct gacctaccac cggaacaacg gccatctcca acttgcaagt
     2041 tggggaaatt aagagcatcg caggtttaat ggaagaaaaa aaaaaggtac agcacagcgc
     2101 aaatggagtt agttccctta tgtcacacac tcacacacag tcggtcagat caagcatact
     2161 gggtgcgtat aaatagagtg gccattgcca ccctgtttat ctcaaaatct gtcttgttag
     2221 tggtcttctc cctttttcag gttacaattc tcttgtttct acttagtata taagtatatc
     2281 aagctatatt aagcatacta tcaactgtca actctatcct caaaatacaa tacaaaatgg
     2341 ctaccatgag cgacgtctgg gttggcctgc catggccagt acaattgatc gcgacctgct
     2401 ttgtcctcac tgcaactctg ttgatcacga ctcgcctcaa ttcactctcc cattcatggc
     2461 cagcggcagc aggaaaaggc cacgaacctc caatagcgcc ctactggctt ccaggaatcc
     2521 agcatctggc agccttcctt ctcgatcctg cccacacttt ccgcacgacc cagtcgaaat
     2581 acaaagattc gccttacaca ttgctcatgg ggaacgtgaa gttccacgtt tttggttctc
     2641 catctgccgc gacccatgtc tttgcacgct cgcgcacgtt tgcgtatgaa ccggtgacct
     2701 tgagcatgct ggagaatgga ctggatctgc ccgtggccga ccgagtttac tttcaaatcg
     2761 gtctggatcg agcaaatagt gcagagcatg cgaaggggtt cgtggtgcaa aaccataatg
     2821 tctggttgcg atacttgtca ggcgagccgt tggacgacct gatgcagcta ttcacacgcc
     2881 acttccacca aatcctggag caacacctgg atatgaagac aagaggctgg cagacagtcg
     2941 atctatacca agtcctgcgc aaagtcattt tcgacacctc tgtcctgacg ttcttcggtc
     3001 cccatcttgc tcagatatgg ggtcctacca tgtggaagga cttctgcctg ttcaacgatg
     3061 ccacctatat tggtgtcaga acaaatttgg cgtatgtctt gcagccgcgc gctggccgcg
     3121 cgcgggagag aatgctacgc gcctttgaac agtggttaac gcaccattca gagggagact
     3181 ggccggacaa ggacaaatat tggaacgagg aatggggcgc aaaaatgaat tgggaacgtg
     3241 accatctggc gcgacaattc ggcctctccc tccgtggacg ggcggccatg caggcgagct
     3301 tcctgtttgc aatcgttctg aacgccgcgc cgatgagttc ctggtttacc tgggtggcgg
     3361 cgagtaattc agatcgcctg gcaaagtgtc gagtcgagtc gtccaagttc ttgcgaccga
     3421 gcaaatcaag ccgcttcgat cacgaattgg acatcgacgc cacgcaaatg agaggcagca
     3481 acttcatgca ggcgttgtgg aaggaagcgt tacgcatagg cacggcagcc tcgccagcac
     3541 gtgtagtcat ggaagacaca gagctggaag ggtatgtcgt aaggaagggc agtgtgatcc
     3601 tcatgcccac tgcgttgctc caccatagcg acttgttccc gagagcggac caggttgaca
     3661 cggaaaggtg gacaggcgag gatgccatag tccaacagcg gagtaaaagt attcgcacat
     3721 ttggtggagg tatgggcatg tgttctggac ggcatgcggc cgagcaggag gtcatcggtc
     3781 tggtctcgaa gcttattacg ctgtttgata tcgagttcga agactcgtgg aagaacaagt
     3841 tccacttcga cccaagaagt ctcggaatta tgcatcctgc aggtcctcta atggtacgcc
     3901 tgaagcggag agtattatga acaaatcgct cttaaatata tacctaaaga acattaaagc
     3961 tatattataa gcaaagatac gtaaattttg cttatattat tatacacata tcatatttct
     4021 atatttttaa gatttggtta tataatgtac gtaatgcaaa ggaaataaat tttatacatt
     4081 attgaacagc gtccaagtaa ctacattatg tgcactaata gtttagcgtc gtgaagactt
     4141 tattgtgtcg cgaaaagtaa aaattttaaa aattagagca ccttgaactt gcgaaaaagg
     4201 ttctcatcaa ctgtttaaaa ggaggatatc aggtcctatt tctgacaaac aatatacaaa
     4261 tttagtttca aagatgaatc agtgcgcgaa ggacataact cattcaattc aattcatcat
     4321 ttttttttta ttcttttttt tgatttcggt ttctttgaaa tttttttgat tcggtaatct
     4381 ccgaacagaa ggaagaacga aggaaggagc acagacttag attggtatat atacgcatat
     4441 gtagtgttga agaaacatga aattgcccag tattcttaac ccaactgcac agaacaaaaa
     4501 cctgcaggaa acgaagataa atcatgtcga aagctacata taaggaacgt gctgctactc
     4561 atcctagtcc tgttgctgcc aagctattta atatcatgca cgaaaagcaa acaaacttgt
     4621 gtgcttcatt ggatgttcgt accaccaagg aattactgga gttagttgaa gcattaggtc
     4681 ccaaaatttg tttactaaaa acacatgtgg atatcttgac tgatttttcc atggagggca
     4741 cagttaagcc gctaaaggca ttatccgcca agtacaattt tttactcttc gaagacagaa
     4801 aatttgctga cattggtaat acagtcaaat tgcagtactc tgcgggtgta tacagaatag
     4861 cagaatgggc agacattacg aatgcacacg gtgtggtggg cccaggtatt gttagcggtt
     4921 tgaagcaggc ggcagaagaa gtaacaaagg aacctagagg ccttttgatg ttagcagaat
     4981 tgtcatgcaa gggctcccta tctactggag aatatactaa gggtactgtt gacattgcga
     5041 agagcgacaa agattttgtt atcggcttta ttgctcaaag agacatgggt ggaagagatg
     5101 aaggttacga ttggttgatt atgacacccg gtgtgggttt agatgacaag ggagacgcat
     5161 tgggtcaaca gtatagaacc gtggatgatg tggtctctac aggatctgac attattattg
     5221 ttggaagagg actatttgca aagggaaggg atgctaaggt agagggtgaa cgttacagaa
     5281 aagcaggctg ggaagcatat ttgagaagat gcggccagca aaactaaaaa actgtattat
     5341 aagtaaatgc atgtatacta aactcacaaa ttagagcttc aatttaatta tatcagttat
     5401 taccctatgc ggtgtgaaat accgcacaga tgcgtaagga gaaaataccg catcaggata
     5461 ggaaaaaacc gagcttcctt tcatccggcg cggctgtgtt ctacatatca ctgaagctcc
     5521 gggtatttta agttatacaa gggaaagatg ccggctagac tagcaagttt taggctgctt
     5581 aacattatgg ataggcggat aaagggccca aacaggattg taaagcttag acgcttctgg
     5641 ttggacaatg gtacgtttgt gtattaagta aggcttggct ggggatagca acattgggca
     5701 gagtatagaa gaccacaaaa aaaaggtata taagggcaga gaagtctttg taatgtgtgt
     5761 aacttctctt ccatgtgtaa tcagtatttc tacttacttc ttaaatatac agaagtaaga
     5821 cagataacca acagcctttc ccagatatac atatatatct ttatttcagc ttaaacaata
     5881 attatatttg tttaactcaa aaataaaaaa aaaaaaccaa actcacgcaa ctaattattc
     5941 cataataaaa taacaacatg ctctcttccg accgcaccca atatactctg gcactctggc
     6001 tgacgcccgt cgtcctctat gtcgtgtacg cgtggctcaa agtcatcacc ggcgccttct
     6061 ttggcccttt gagcaaaatt cccggcccca agctctgggc catctcgcgc cttcctcgta
     6121 tgtacatgga atggcagggt gaggaagcag ctggagtcgc cgcgttgcac aagaagtacg
     6181 gcccggtcgt gcggctggcg cccaacgaag tgtcgtttgc tggtggtgct caggcttgga
     6241 aagacattta tggctaccga aagaacgtgg gccaagagaa gcatccatat cgttcgtcgg
     6301 cgtcgtattg gcctccggcc aacggagccc cgagcatgat tcaggccatc cgtgacgaag
     6361 accaccagcg actgcgcaag agtctggccg tctcgttcag tgagaagtcc atgaagctga
     6421 tggaaccacg cgtcaagggc tgggctcggc ttctgcagac caagttggct gagcgcggtg
     6481 atgctgcggt ggatatggtc gagttgttta gatgtgcaac tttcgatgcc atgggtgacc
     6541 tcgcgctagg ggacgacctg caaatgctga gaaatggccg tctctccgac tacgtccatg
     6601 cagcttttag tggcattcga accatcatcc gccttcgggt cctctccacg tacaattcct
     6661 tcacccgctg gctcgttcaa gactgtttct tccaaagcgc attcgtccgg aaggccgcca
     6721 tggaaaatct tcgctactcc gcggaccgtg tggatcgtcg cctgcaagaa ccagaccgag
     6781 accctcccga tctctggaca aaacccctga ctggtatcga cggcaagatg tccctggacg
     6841 aatatcactc caccgcacag cttctcatga ctgcgggcac cgacacttcg gcgataggct
     6901 tgtccggcac actatattat cttcttacca accccgactg cctcgccaag gtcactgacg
     6961 aggttcgctc cgccttctca tctatggacg acgtaaccta cgaaaccctc gcccaactga
     7021 agtacttgga cgccgcgctg caagaaggcc tgagactcta cccaccggtg cctaccggcg
     7081 cacctcgcgt cactccgatg cctggatggt ccatcggcgg ctactggatc ccaggtggtg
     7141 tgaacgtcca cgcgccccac tatgccacct ctcgccttcc cgcccacttc accgctcccg
     7201 aatctttcca cccagagcgc tggctgggcg acgagaaatt ccaaaacgac cggcttgatg
     7261 ccgtccagcc gttcaacctc ggccctcaga actgcttggg tcaggctttt gccatgaacg
     7321 agatgcgcct cttcatggcc gctgtgttgc tccaattcga tctacagttg tgcgaggacc
     7381 cccgagcctg gttggatcaa cgagtgttct ccatgtggga gacgaagccg ttgatgtgtg
     7441 tcgcgaccaa gacgagctga agtgctttta actaagaatt attagtcttt tctgcttatt
     7501 ttttcatcat agtttagaac actttatatt aacgaatagt ttatgaatct atttaggttt
     7561 aaaaattgat acagttttat aagttacttt ttcaaagact cgtgctgtct attgcataat
     7621 gcactggaag gggaaaaaaa aggtgcacac gcgtggcttt ttcttgaatt tgcagtttga
     7681 aaaataacta catggatgat aagaaaacat ggagtacagt cactttgaga accttcaatc
     7741 agctggtaac gtcttcgtta attggatact caaaaaagat ggatagcatg aatcacaaga
     7801 tggaaggaaa tgcgggccac gaccacagtg atatgcatat gggagatgga gatgatacct
     7861 ccattgggcc gatgaagtta gtcgacggat agaagcggtt gtcccctttc ccggcgagcc
     7921 ggcagtcggg ccgaggttcg gataaatttt gtattgtgtt ttgattctgt catgagtatt
     7981 acttatgttc tctttaggta accccaggtt aatcaatcac agtttcatac cggctagtat
     8041 tcaaattatg acttttcttc tgcagtgtca gccttacgac gattatctat gagctttgaa
     8101 tatagtttgc cgtgattcgt atctttaatt ggataataaa atgcgaagga tcgatgaccc
     8161 ttattattat ttttctacac tggctaccga tttaactcat cttcttgaaa gtatataagt
     8221 aacagtaaaa tataccgtac ttctgctaat gttatttgtc ccttattttt cttttcttgt
     8281 cttatgctat agtacctaag aataacgact attgttttga actaaacaaa gtagtaaaag
     8341 cacataaaag aattaagaaa atgtcccaca acggcgagcc cgacccgctc taccgcgagc
     8401 cctatcccct taaccgcgac ttgctcgcct ccgcccgcct gcacctccag cacgccgtct
     8461 tcgtcaacgg tctaggctac ctgctgcacc cggccgtccc tatcgggccc gcctcgcacg
     8521 tggctgacat cgccaccggc accggcatct tcgccctcgc cctcgccgcg gcccaccccg
     8581 aagcccgcgt cgaagcctcc gacatctccc tcgcccaggt ccccgcccag cactggtggc
     8641 ccgccaatgt gacctttgcg gtcctcgaca tcttccagga ccccttgccg gcctcactgc
     8701 tcgcgcgcta cgacgtcctc tgcctccgcc acttcgtctc cctgcagccc ggcgatccgc
     8761 gcccgctgat cgcccgcttg ctggccatgc tgcggcccgg gggctatctg cactggcagg
     8821 aatgggacct gaccaccgcc gccgtcttcg ccccagacgg cacctccacc cctaagatgc
     8881 aggcgctgat cgattacatc aaacatccct cccgacacac cgcccaggcc acctgggttg
     8941 gcgacttgca tcagagggtc gccgccggcg aggtgcctgg cgtggagctg gtcgctcacg
     9001 accgtgtccg cgccgctaag gccgccgtcg tcgctatgca gcaggaactc atggcgctgg
     9061 gcaccaagga ggtagtcgcc agtctgcggg cccgcgaggg cgaagcgagc gtcgaggccg
     9121 ctaagttcga gaagatcgcg cacgacgccg tcgacgagtg tctgagcttg gggaggggcc
     9181 ctgtgatcga tcaggagatg gtgacgtggg tgctgaggaa gaagtgagga gattgataag
     9241 acttttctag ttgcatatct tttatattta aatcttatct attagttaat tttttgtaat
     9301 ttatccttat atatagtctg gttattctaa aatatcattt cagtatctaa aaattcccct
     9361 cttttttcag ttatatctta acaggcgaca gtccaaatgt tgatttatcc cagtccgatt
     9421 catcagggtt gtgaagcatt ttgtcaatgg tcgaaatcac atcagtaata gtgcctctta
     9481 cttgcctcat agaatttctt tctcttaacg tcaccgtttg gtcttttata gtttcgaaat
     9541 ctatggtgat accaaatggt gttcccaatt catcgttacg ggcgtatttt ttaccaattg
     9601 aagtattgga atcgtcaatt ttaaagtata tctctctttt acgtaaagcc tgcgagatcc
     9661 tcttaagtat agcggggaag ccatcgttat tcgatattgt cgtaacaaat actttgatcg
     9721 gcgctatgcg gccgccaccg cggtggagct ccagcttttg ttccctttag tgagggttaa
     9781 ttgcgcgctt ggcgagactc aaacaagata tattttgccc gccttacaga cgaaactagt
     9841 tggaggttat ggagcatact atcacgtggg cggccactgg tgagttacta caccccaggg
     9901 gcaacgttga tgctcctaaa aaactctggc tggacgcaag ccgtaacacc cgtgtcactt
     9961 cataatcgtt tgcaattcag ggcttgatct acacattacc ctgttatccc tattaattaa
    10021 tacggttatc cacagaatca ggggataacg caggaaagaa catgtgagca aaaggccagc
    10081 aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg ctccgccccc
    10141 ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat
    10201 aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc
    10261 cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct
    10321 cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg
    10381 aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc
    10441 cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga
    10501 ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa
    10561 ggacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta
    10621 gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc
    10681 agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg
    10741 acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgagatta tcaaaaagga
    10801 tcttcaccta gatcctttta aattaaaaat gaagttttaa atcaatctaa agtatatatg
    10861 agtaaacttg gtctgacagt taccaatgct taatcagtga ggcacctatc tcagcgatct
    10921 gtctatttcg ttcatccata gttgcctgac tccccgtcgt gtagataact acgatacggg
    10981 agggcttacc atctggcccc agtgctgcaa tgataccgcg agacccacgc tcaccggctc
    11041 cagatttatc agcaataaac cagccagccg gaagggccga gcgcagaagt ggtcctgcaa
    11101 ctttatccgc ctccatccag tctattaatt gttgccggga agctagagta agtagttcgc
    11161 cagttaatag tttgcgcaac gttgttgcca ttgctacagg catcgtggtg tcacgctcgt
    11221 cgtttggtat ggcttcattc agctccggtt cccaacgatc aaggcgagtt acatgatccc
    11281 ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc agaagtaagt
    11341 tggccgcagt gttatcactc atggttatgg cagcactgca taattctctt actgtcatgc
    11401 catccgtaag atgcttttct gtgactggtg agtactcaac caagtcattc tgagaatagt
    11461 gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg ggataatacc gcgccacata
    11521 gcagaacttt aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa ctctcaagga
    11581 tcttaccgct gttgagatcc agttcgatgt aacccactcg tgcacccaac tgatcttcag
    11641 catcttttac tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa
    11701 aaaagggaat aagggcgaca cggaaatgtt gaatactcat actcttcctt tttcaatatt
    11761 attgaagcat ttatcagggt tattgtctca tgagcggata catatttgaa tgtatttaga
    11821 aaaataaaca aataggggtt ccgcgcacat ttccccgaaa agtgccacct gaacgaagca
    11881 tctgtgcttc attttgtaga acaaaaatgc aacgcgagag cgctaatttt tcaaacaaag
    11941 aatctgagct gcatttttac agaacagaaa tgcaacgcga aagcgctatt ttaccaacga
    12001 agaatctgtg cttcattttt gtaaaacaaa aatgcaacgc gagagcgcta atttttcaaa
    12061 caaagaatct gagctgcatt tttacagaac agaaatgcaa cgcgagagcg ctattttacc
    12121 aacaaagaat ctatacttct tttttgttct acaaaaatgc atcccgagag cgctattttt
    12181 ctaacaaagc atcttagatt actttttttc tcctttgtgc gctctataat gcagtctctt
    12241 gataactttt tgcactgtag gtccgttaag gttagaagaa ggctactttg gtgtctattt
    12301 tctcttccat aaaaaaagcc tgactccact tcccgcgttt actgattact agcgaagctg
    12361 cgggtgcatt ttttcaagat aaaggcatcc ccgattatat tctataccga tgtggattgc
    12421 gcatactttg tgaacagaaa gtgatagcgt tgatgattct tcattggtca gaaaattatg
    12481 aacggtttct tctattttgt ctctatatac tacgtatagg aaatgtttac attttcgtat
    12541 tgttttcgat tcactctatg aatagttctt actacaattt ttttgtctaa agagtaatac
    12601 tagagataaa cataaaaaat gtagaggtcg agtttagatg caagttcaag gagcgaaagg
    12661 tggatgggta ggttatatag ggatatagca cagagatata tagcaaagag atacttttga
    12721 gcaatgtttg tggaagcggt attcgcaata ttttagtagc tcgttacagt ccggtgcgtt
    12781 tttggttttt tgaaagtgcg tcttcagagc gcttttggtt ttcaaaagcg ctctgaagtt
    12841 cctatacttt ctagagaata ggaacttcgg aataggaact tcaaagcgtt tccgaaaacg
    12901 agcgcttccg aaaatgcaac gcgagctgcg cacatacagc tcactgttca cgtcgcacct
    12961 atatctgcgt gttgcctgta tatatatata catgagaaga acggcatagt gcgtgtttat
    13021 gcttaaatgc gtacttatat gcgtctattt atgtaggatg aaaggtagtc tagtacctcc
    13081 tgtgatatta tcccattcca tgcggggtat cgtatgcttc cttcagcact accctttagc
    13141 tgttctatat gctgccactc ctcaattgga ttagtctcat ccttcaatgc tatcatttcc
    13201 tttgatattg gatcatacta agaaaccatt attatcatga cattaaccta taaaaatagg
    13261 cgtatcacga ggccctttcg tctcgcgcgt ttcggtgatg acggtgaaaa cctcggcgcg
    13321 ccattaccct gttatcccta atatacaacg caaagaagct ggaaaattgg cgagggaatc
    13381 ctgtttctgt ctatccaaga atgggcatga ggtggcaacc gtcgtgctag cgtacagggt
    13441 gcactttgta accatttggg acaccggaca ctcgctgttt tcgaaattac cctttaagcg
    13501 cgggtattga accaggctta tgcccaagat cgtagcaagc atttaaatga cggtatcgat
    13561 aagcttgata tcgaattcct gcagcccggg ggatccacta gttctagagt taattaa
//
