LOCUS       sequence-208186-       15221 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of a fungal biosynthetic gene cluster.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 15221)
  AUTHORS   Harvey CJB, Tang M, Schlecht U, Horecka J, Fischer CR, Lin HC, Li J,
            Naughton B, Cherry J, Miranda M, Li YF, Chu AM, Hennessy JR, Vandova
            GA, Inglis D, Aiyar RS, Steinmetz LM, Davis RW, Medema MH, Sattely 
            E, Khosla C, St Onge RP, Tang Y, Hillenmeyer ME
  TITLE     HEx: A heterologous expression platform for the discovery of fungal 
            natural products.
  JOURNAL   Sci Adv. 2018 Apr 11;4(4):eaar5459. doi: 10.1126/sciadv.aar5459. 
            eCollection 2018 Apr.
  PUBMED    29651464
REFERENCE   2  (bases 1 to 15221)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Adv."; 
            date: "2018-04-11"; volume: "4(4):eaar5459. doi"; pages: " 
            10.1126/sciadv.aar5459. eCollection 2018 Apr"
COMMENT     Sequence Label: pCHKU32.1-2.2
FEATURES             Location/Qualifiers
     source          1..15221
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             504..527
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     primer_bind     complement(9656..9676)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(9656..9674)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     rep_origin      complement(10013..10601)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(10093..10112)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(10772..11563,11564..11632))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     11395..11414
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(11633..11737)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      11764..13106
                     /label=2u ori
                     /note="yeast 2u plasmid origin of replication"
     protein_bind    complement(12727..12774)
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     primer_bind     13147..13165
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        13437..13841
                     /gene="S. cerevisiae LEU2"
                     /label=LEU2 promoter
     CDS             13854..14948
                     /codon_start=1
                     /gene="S. cerevisiae LEU2"
                     /product="3-isopropylmalate dehydrogenase, required for 
                     leucine biosynthesis"
                     /label=LEU2
                     /note="yeast auxotrophic marker"
                     /translation="MSAPKKIVVLPGDHVGQEITAEAIKVLKAISDVRSNVKFDFENHL
                     IGGAAIDATGVPLPDEALEASKKVDAVLLGAVAGPKWGTGSVRPEQGLLKIRKELQLYA
                     NLRPCNFASDSLLDLSPIKPQFAKGTDFVVVRELVGGIYFGKRKEDDGDGVAWDSEQYT
                     VPEVQRITRMAAFMALQHEPPLPIWSLDKANLLASSRLWRKTVEETIKNEFPTLKVQHQ
                     LIDSAAMILVKNPTHLNGIIITSNMFGDIISDEASVIPGSLGLLPSASLASLPDKNTAF
                     GLYEPCHGSAPDLPKNKVDPIATILSAAMMLKLSLNLPEEGKAIEDAVKKVLDAGIRTG
                     DLGGSNSTTEVGDAVAEEVKKILA"
     primer_bind     complement(15196..15212)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(15196..15212)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
ORIGIN
        1 tatctaaaaa ttgccttatg atccgtctct ccggttacag cctgtgtaac tgattaatcc
       61 tgcctttcta atcaccattc taatgtttta attaagggat tttgtcttca ttaacggctt
      121 tcgctcataa aaatgttatg acgttttgcc cgcaggcggg aaaccatcca cttcacgaga
      181 ctgatctcct ctgccggaac accgggcatc tccaacttat aagttggaga aataagagaa
      241 tttcagattg agagaatgaa aaaaaaaaaa aaaaaaaagg cagaggagag catagaaatg
      301 gggttcactt tttggtaaag ctatagcatg cctatcacat ataaatagag tgccagtagc
      361 gacttttttc acactcgaaa tactcttact actgctctct tgttgttttt atcacttctt
      421 gtttcttctt ggtaaataga atatcaagct acaaaaagca tacaatcaac tatcaactat
      481 taactatatc gtaatacaca atggattata aggatgatga tgataagact agtatgggta
      541 gtaatgccaa ccccaactgg ctcgcgaagg agcctatcgc gatagtggga agctcgtgtc
      601 gttttccagg cggcgctagt tccccctcaa agctttgggt gcttctcaag gatcctcggg
      661 atatcgtgca agaaatccca cccagtcgat ttgataccaa ggcattttat caccaagaca
      721 gccaacacca tgggagtaca aacgcaaagt atgcctacct cctgcaggat gacccccggg
      781 ctttcgaccg cgatttcttt aacatcagcc caaaggaggc cgaggcaatg gacccccaac
      841 agcgatgcct cctcgagaca gtttacgaag gtattgaatc ggccggctac tccatccccc
      901 agcttcgggg atcatccact ggcgtctttg tgggagccat gagcttcgac taccagctcg
      961 ttgccatgcg tggcctcgac agcctgccgc agtaccatgc aaccggcggt tcgatggcaa
     1021 tcctggccaa ccgtgtgtcg tacttctatg actggaaagg cgcatcggtt gcctttgata
     1081 cagcctgctc gtcatcgctc gttgccctac accaggcggt ccttgctctg cgaagcggag
     1141 aagtagacat ggcggttgca gcaggggcga atctcatcct tggacctgag ccatttattg
     1201 cttattcaaa tctaaatatg ctgtccccca acgggcgatc atacatgtgg gattcttctg
     1261 ccgatggata tacgcgcggc gagggattcg ctgccattat tctcaagact cttagccaag
     1321 ctgtggctga tggtgaccac atcgagtgta ttatccgtga aacgggtgtg aattccgatg
     1381 gccggacacc aggcatcaca atgccgaact ctgaggcaca agctcgactt atccgagaca
     1441 catacaccaa gtgcgggcta gacccgacga acccaaggga ccgtccacaa tacttcgaag
     1501 cacatggcac cggaacgccg gtgggtgacc ctatcgaggc gcgggcaata cacgacgctt
     1561 tcttcccggg gcatccagac cagaatcatg gccagctcta tgttggatcg atcaaaaccg
     1621 tcattggtca cacagagggc agtgcaggac tggctggcgt gctcaaggcg tcgttggcgg
     1681 tccagcacgg ccagattcca ggtaacctac atcttcggga gctgaacccc aaaattcgac
     1741 cattcttcaa ccacctccgc atcccgacag agttaacccc ctggcccgcg cttccagagg
     1801 gccaaccgcg tcgtgcaagt gtgaatagtt tcggctttgg cgggaccaat gcgcacgcta
     1861 tcatcgaaag ctgggatggg ggctacgagc gctatcatgt gtcggaatcg cgtccggctg
     1921 gactcttcgt cctatctgct aactcggccc aggccctggc agcaaaggct gcagagttag
     1981 ccaactatct acgagatcat cccgacacag atcttggtag gctttctcgc accctgttcc
     2041 agaggtcgga attcccgttc cgggcctcct tctcagccac gtcggccacc cagcttgccg
     2101 ataagctcga aacaggcaca gaagcactca gcaagacgtc acgcatcgca accattccca
     2161 attcactacc gccccggata ctcggtgtct tcactgggca aggtgctcag tgggctacta
     2221 tgggttggga gctctaccaa gcatccgccg tgttccgcac gtcgttggac cggcagcagc
     2281 gaagcctcga tacgctcccc gacggcgatc gcccggactg gtccctgatc gacgagctct
     2341 cggcaccaaa ggaaacctcc cgcgtcggta tcgcagccat ctcccagcct ctctgcactg
     2401 ctctccaagt ggcgctcgta gacgtcctcc gcacggctgg cgttgagttt tctgccgttg
     2461 ttggacactc gtcgggtgag attggtgccg cctatgctgc gggatacctg gatgcccggg
     2521 atgctattcg tgtggcttat tatcgagggt ttcattctca tctggcacag gggcccggag
     2581 gaaagcgtgg caagatgatg gctgttggca tgagcttgga tgaggcattg gcattttgca
     2641 acgagtttag ttcggcactc aaggtggctg ctaacaactc tccaacgagt tgcacgctgg
     2701 cgggagacgc cgatgctatc gatgaggcca aggcactgct ggaccagagc aacacttttg
     2761 cccgcgtgct ggcggtcgac acagcatatc attctcacca catgcggcca tgcgcctcac
     2821 cctatctcga atcgctacgc agttgtggga tcagcgttct gaagggtcgc aaaaaatgcg
     2881 tgtggtactc gagtgtttgg ggtgcaaatg gccgcagtcg ctccttcgac gaccatcatg
     2941 gtcttgagtt gctcggggga cagtactggg tcgataatct gaccagtacg gtgcagttct
     3001 cccaggcagt aagcagagca gtgtctgaag aatcctacgt cttggatctc gcccttgaaa
     3061 ttggaccgca cccggctctc aggggtccag cctccgaggt gatcaagtcg ttgacgggtg
     3121 tagctgttcc gtattctggt gtccttaaac gcggagaggg tgctgttgag gcctttgccg
     3181 ctggtttagg actcatctgg tcgttgttcc cgtcgatgcg ccctatcatc acctttgacg
     3241 gcttacaccg agccttcccc caagccagcc caaagtatcc tgtaattctc aaggacctgc
     3301 cgccttaccc ctgggatcat gatagcctca tctggcgtga gtccagagca tcttgcatct
     3361 tccgcactca gagccagcct cgcgaactcc tcggctttcc ggtcacactc ggtgagcatg
     3421 gccggcgcga ggttcattgg cgacaggtct tcaaactaag tgaactgcaa tgggtgaagg
     3481 gccatgccat ccagggcgaa gttcttttcc cggcaacggg gtacctgacc atggcgtatg
     3541 aggctgctgt gcgtctcgtc aatgaccatc aggcactgcg tcttgtcgag ctgcatgaca
     3601 ttgagatggt gcgtgccatg agcttgcagg aagattcccc gggtcttgag gttctcttta
     3661 ccatacgtgt tacgagccag tccgacagct gcattgccgc cgaggtggcc tgctacagcg
     3721 gcgatatcaa cgtgacgaaa ctggacggcc ctttgacagg gcttacagcc catttcagcg
     3781 gcagcgtgcg gatttggctt ggcctgcccc tgaaaaacgc cctgcctccc cggacggagc
     3841 ctctgctgcc catgaacacg ttggacctgg agcagttcta ctggtatttg tccaaagtgg
     3901 gctacaacta ctcagatgct ttcagagcca gctcagtgga ccgccgcctt aaccaggccg
     3961 tggtaaccgt cccggctccc cctgagccga ctacaattcg gtcttctatg caccccacgg
     4021 tcctcgacac tgccatccaa ggcctcttgg ccgggttttc atatccagaa gacggtcgtc
     4081 tcaggacggt gtatctgcca accgccattg actgcatccg tatcaacatg gtggcacccc
     4141 ctgcgtctcc cggcgtcctc atggcagata gctttctgac cttggcggat gcaaagtcac
     4201 ttgaaggcga cgtagatgtt ttcgactcaa aagatgccac catccatgtc caactgcgcg
     4261 gagtccactg gaccgccctg aaccaggagg aagatcgctg gctctatgca gccgagacat
     4321 gggtgcgaga tgcggcttac gggattgagc ccaggctcaa gaccaaactc tctccagaga
     4381 atagtttgtt acgcacgctt cttgtgcgaa ccgcttactt ctacctgcgg agtcttcgag
     4441 atcagatcaa accctctgag cttttactta tgagcaaaca tcggcggcac atgatgaagt
     4501 gggtcaggga gcatctattt ccccagatcg aagccggcaa gcaccccgat attcaagcag
     4561 agtggtccaa cgacactctc gaggacgtgg agcaatggag cgccagctat ttggcttcag
     4621 gaaacaatga tatgcagcta ctacatgccg tgggtggtaa gctgccagcc atagctcgcg
     4681 gaactattcc tcctctccag gtactgatga aggacggtat gctcgaccgt ttgtatgtgg
     4741 agggagtcgg gtttgctgac ggcaacctcg atctcgagtc cttggttaag cagcttgcac
     4801 atcggtaccc tcgtatgaag gtgctcgaag tcggtgctgg gacgggtggt actactcgag
     4861 cggtgctttc ggctttggac ggcaaataca catcatatac gtacacggat atctcggccg
     4921 gcttctttga gcctgcgaaa gcaaagttca gccagcatct gagtcagctg tcgttcaaga
     4981 tgctcaacat cgaaaaggac ccggtagagc aaggattcga ggagggctcg ttcgatatgg
     5041 tcgttgcctc gaactgcctc cacgcaacac ggagcctgaa ggataccgtc cgccactgtc
     5101 gccgcttact tcgacctggt gggtacttgg tgctgctgga gatcacacgc gaccatcttc
     5161 caatccagct catcatgggt acgcttcctg ggtggttcct gggcgccgat gagggacgag
     5221 tctgggctcc cacgatcggc ctcgacgagt gggatgcttt actgaaggtc accgggtttt
     5281 caggggtgga cacaagctcg acgccttcct tctgttcggt catcttatcc caggcaaccg
     5341 acgaaaccat ccagatgctc cgtcagcccc tttccaccaa ttctcagtcg ccaatgccgc
     5401 ctctcgacca acaagtcctg gtcgtcggcg gttctgaact cgcatccagg tcgcaaacac
     5461 tcctcgcttc tgccggggct gtcatcagac gtaaccttga gggcattgag attcccagcg
     5521 gtgccgttgt gctctgcctt tgtgacctgg acagcccggt ctttaactcc atgagccagt
     5581 cgaggttcga agccgtgcag gccgtcttcc gaaacgccag tactgttcta tgggtgagtt
     5641 caggggcgac ttctggcaag aatcccctgg ccaacgtgac cgtgggattg ggacggactc
     5701 ttctggccga acgtagtgac ctgaggcttc agttcctcga cgtcgatgcg cccgcaaccc
     5761 ttgaaccgtc tttactagca actcttctcc tccgacttac cgggatgaat tcagactaca
     5821 attcggacga gatcctctgg acccctgagc cccagcttac actaagggac ggtgctatat
     5881 atatacccag ggttctggct ctggatgcta tcaatcttcg gtccacggcg aggcaccgac
     5941 aagtcaccca gctgaccagc tttgagcttc cagatatagt catcgaggtc acgaagcgac
     6001 acggagcact cgaactgctg tatcgccatc accatgagca tgtccacaat gttaagccag
     6061 gcgaggtgcg tgtgaaggtc acggcatcgt ctctttactc ccttgtctgc tgcgaaggtt
     6121 cgtccaagat gtctgcatct gcatcttcga ggcctgctca tctgtgggtg ggccacgaca
     6181 tggtatcagg ggataaactc gtcggccttt cacacgcaag cagctccatc gtcacggttg
     6241 ctgaggattg cgtcttgtat cgccaacgca gtgccgccaa caccgccgcc gaggagtcgg
     6301 tcgacgccgc gcaacagctc caccatttca cagctcaggc gctagtgaag catctccttc
     6361 gcgacttgga gggccccgtc tggattcacg gcgccccaaa tcatctcagc aaggcaattt
     6421 cccgtgttgc tgcagagcag tgtctcaccg tgttcgagac gacgtcggat atggagagag
     6481 ctggcgactg gggtttcatc catccctaca tcagcaagcg aaatcttcaa tcactacgca
     6541 tgcgcatgag agacgtgaaa acgttcatcg acttgaagcc ctggaagagc aacagtattt
     6601 cggcgctgct gcgtgcctcg tctccacgtt ccaggctcgt taccatcgac atcgaagatc
     6661 tgaatgatct gattattgga ttgagccgcg aggacctatg caggctggcc agacagcact
     6721 ttgaagacca tgacaacatc cggaggggcc cggataacct agaccagatc ctgcccatcg
     6781 aaaacgtaac aacggaggta gtcaaggggt tgggccctgc aacggtgatt gactggtccg
     6841 ctaccgacaa agtcaccacc gtggtccatc ctgtcgagca caacgggctc tttgccgcta
     6901 acaagacata tcttctcttc ggcatgacgg gagatgtagg catttcaatt gctctatgga
     6961 tggtggatca cggagctcgg agcgtcgtcc tggccagccg taagcccagt gtgccagcca
     7021 gtgtggttga gcatatgtcg caaaaggggg cagacctgcg tgtcatggcc gttgacatta
     7081 cgaacagaga tgctctgaga accttttatg ccgacatcaa gtccagcatg ccgccggtcg
     7141 gcggcgtcat caatggtgcg atggtgcttc gggaccgtct ctttgtcgac atgtcctggc
     7201 ccgactttga ggccgtgctg gcgcccaagg ttgtgggaac tcagaatatt gatgaactct
     7261 ttggcgacgg tgaagaggat gagactctgg acttcttcat cgtcctctcg tcggcgactt
     7321 cgctggtcgg cattatcggc caatccgcct acagtgctgc gaaccacttc gcggccagcc
     7381 tggtgcgcca gcgtcgcagt cgtggcctgg cgggttctgt catcgtcatt gggttcctga
     7441 cggggctggg ctacatcttc catcgttcag acaaggaaca cctcgcgacg attgaaaagt
     7501 cacttctgcc acggctggat agacaatccg agacagatct gtgcgagatg ctggccgagg
     7561 ccattgtgtg cggacgaccc aactcagacc agccacccga gctaatcaca ggtataagga
     7621 cgtccttcca agaggcatgg caccaagacc cgcggctttc gtgctatctc gtccaagaag
     7681 gagttcgaga gagcggcggt ggaccagagg aggtcaacgg gaacgttaaa gtcgaggttc
     7741 agctggcggc ggtggaggat cccaaagacg ggctgacagt cttggagaag tgcttttccc
     7801 aggctttggg caacatgctc cagctcgacc cagcgcaaat atcgggcgat gttcctgcag
     7861 tcaaccttgg agtcgactcc ctcgtctcgg tccggattcg tgagtggttc ctgaaggagc
     7921 tcggcgtcga cgtacccatc ctgaaaatta tgtccacgaa ccacactgta tctcgactgt
     7981 gtgaggacgc tctgacaggc tggcgaaggc ttcaggcagg gtccgatatg gcggcggcaa
     8041 catcctcttc cggcacagct cgtcaggacc cagagatgga ctggacaaag gaattgaccg
     8101 gacttattga cggaatccct gctcttatcc cgcaaggagc cgagtcggtc aaggatgcgc
     8161 ctcggagaag tgcacggcgc atcgttctca cagggtgcac aggcttcttg ggtacgcaca
     8221 tgcttcgcaa cctggtcgca gatgacaacg ttgctgagct gcactgtctc tgcattcgct
     8281 cacgccgtgt tcgcgtccgg gaccccagga ttcgagagta caaaggcgac ctgatgaagc
     8341 ccctgttggg gctttctaac gacgacttca tccgcctcag ccagactgcc gacttgatca
     8401 tccacttggg cgccgaggtc aaccatctca agtcgtacga ggctgtacgc accgccaatg
     8461 ttgtctcgac acaagttctc ctcgccatgg ccacgccgcg cggtgtcccc gtccatttcg
     8521 tatcgtcatc atcggtcgcc atgctgcaga aggggaccca tgagctgccc gagatacccc
     8581 cgtcagccat ctcccctccg accgatgccg agtccctgat gaagaatgcc ataggctacg
     8641 ctgccagtaa gtggatgggc gagatgctgc tagaacacgc cgcgccgcct gcggttgtgc
     8701 accgcttccc caacatcatg gggcccgatg ctcccgatga gatcccgcta gttgcgctgg
     8761 accgcttctg cacccggatg cgagccgtgc cggcgttgga tccgcagcag tgggtaggac
     8821 aactggatat catcgaggtc agtgatgtcg tgcccgagtt tttggcaaac gcatatgggc
     8881 acgatcccca gaagggtttt gccgtgcaca actattgcag cagtaacgag tactggctgt
     8941 cggacctggc gggcatgtat gagaaaaagc tcggcggcca cattaaagtc ttgccgacgg
     9001 ccgagtggat gcggagagcg acggccatgg ggatgccgaa gggagtagtg gcgacgttta
     9061 ctggccatga cgaagtcttc gtatcgccag tattgcggaa ggggtcaggt tcgaggtaag
     9121 gagattgata agacttttct agttgcatat cttttatatt taaatcttat ctattagtta
     9181 attttttgta atttatcctt atatatagtc tggttattct aaaatatcat ttcagtatct
     9241 aaaaattccc ctcttttttc agttatatct taacaggcga cagtccaaat gttgatttat
     9301 cccagtccga ttcatcaggg ttgtgaagca ttttgtcaat ggtcgaaatc acatcagtaa
     9361 tagtgcctct tacttgcctc atagaatttc tttctcttaa cgtcaccgtt tggtctttta
     9421 tagtttcgaa atctatggtg ataccaaatg gtgttcccaa ttcatcgtta cgggcgtatt
     9481 ttttaccaat tgaagtattg gaatcgtcaa ttttaaagta tatctctctt ttacgtaaag
     9541 cctgcgagat cctcttaagt atagcgggga agccatcgtt attcgatatt gtcgtaacaa
     9601 atactttgat cggcgctatg cggccgccac cgcggtggag ctccagcttt tgttcccttt
     9661 agtgagggtt aattgcgcgc ttggcgagac tcaaacaaga tatattttgc ccgccttaca
     9721 gacgaaacta gttggaggtt atggagcata ctatcacgtg ggcggccact ggtgagttac
     9781 tacaccccag gggcaacgtt gatgctccta aaaaactctg gctggacgca agccgtaaca
     9841 cccgtgtcac ttcataatcg tttgcaattc agggcttgat ctacacatta ccctgttatc
     9901 cctattaatt aatacggtta tccacagaat caggggataa cgcaggaaag aacatgtgag
     9961 caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata
    10021 ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc
    10081 cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg
    10141 ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc
    10201 tttctcatag ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg
    10261 gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc
    10321 ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga
    10381 ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg
    10441 gctacactag aaggacagta tttggtatct gcgctctgct gaagccagtt accttcggaa
    10501 aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg
    10561 tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt
    10621 ctacggggtc tgacgctcag tggaacgaaa actcacgtta agggattttg gtcatgagat
    10681 tatcaaaaag gatcttcacc tagatccttt taaattaaaa atgaagtttt aaatcaatct
    10741 aaagtatata tgagtaaact tggtctgaca gttaccaatg cttaatcagt gaggcaccta
    10801 tctcagcgat ctgtctattt cgttcatcca tagttgcctg actccccgtc gtgtagataa
    10861 ctacgatacg ggagggctta ccatctggcc ccagtgctgc aatgataccg cgagacccac
    10921 gctcaccggc tccagattta tcagcaataa accagccagc cggaagggcc gagcgcagaa
    10981 gtggtcctgc aactttatcc gcctccatcc agtctattaa ttgttgccgg gaagctagag
    11041 taagtagttc gccagttaat agtttgcgca acgttgttgc cattgctaca ggcatcgtgg
    11101 tgtcacgctc gtcgtttggt atggcttcat tcagctccgg ttcccaacga tcaaggcgag
    11161 ttacatgatc ccccatgttg tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg
    11221 tcagaagtaa gttggccgca gtgttatcac tcatggttat ggcagcactg cataattctc
    11281 ttactgtcat gccatccgta agatgctttt ctgtgactgg tgagtactca accaagtcat
    11341 tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata cgggataata
    11401 ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa
    11461 aactctcaag gatcttaccg ctgttgagat ccagttcgat gtaacccact cgtgcaccca
    11521 actgatcttc agcatctttt actttcacca gcgtttctgg gtgagcaaaa acaggaaggc
    11581 aaaatgccgc aaaaaaggga ataagggcga cacggaaatg ttgaatactc atactcttcc
    11641 tttttcaata ttattgaagc atttatcagg gttattgtct catgagcgga tacatatttg
    11701 aatgtattta gaaaaataaa caaatagggg ttccgcgcac atttccccga aaagtgccac
    11761 ctgaacgaag catctgtgct tcattttgta gaacaaaaat gcaacgcgag agcgctaatt
    11821 tttcaaacaa agaatctgag ctgcattttt acagaacaga aatgcaacgc gaaagcgcta
    11881 ttttaccaac gaagaatctg tgcttcattt ttgtaaaaca aaaatgcaac gcgagagcgc
    11941 taatttttca aacaaagaat ctgagctgca tttttacaga acagaaatgc aacgcgagag
    12001 cgctatttta ccaacaaaga atctatactt cttttttgtt ctacaaaaat gcatcccgag
    12061 agcgctattt ttctaacaaa gcatcttaga ttactttttt tctcctttgt gcgctctata
    12121 atgcagtctc ttgataactt tttgcactgt aggtccgtta aggttagaag aaggctactt
    12181 tggtgtctat tttctcttcc ataaaaaaag cctgactcca cttcccgcgt ttactgatta
    12241 ctagcgaagc tgcgggtgca ttttttcaag ataaaggcat ccccgattat attctatacc
    12301 gatgtggatt gcgcatactt tgtgaacaga aagtgatagc gttgatgatt cttcattggt
    12361 cagaaaatta tgaacggttt cttctatttt gtctctatat actacgtata ggaaatgttt
    12421 acattttcgt attgttttcg attcactcta tgaatagttc ttactacaat ttttttgtct
    12481 aaagagtaat actagagata aacataaaaa atgtagaggt cgagtttaga tgcaagttca
    12541 aggagcgaaa ggtggatggg taggttatat agggatatag cacagagata tatagcaaag
    12601 agatactttt gagcaatgtt tgtggaagcg gtattcgcaa tattttagta gctcgttaca
    12661 gtccggtgcg tttttggttt tttgaaagtg cgtcttcaga gcgcttttgg ttttcaaaag
    12721 cgctctgaag ttcctatact ttctagagaa taggaacttc ggaataggaa cttcaaagcg
    12781 tttccgaaaa cgagcgcttc cgaaaatgca acgcgagctg cgcacataca gctcactgtt
    12841 cacgtcgcac ctatatctgc gtgttgcctg tatatatata tacatgagaa gaacggcata
    12901 gtgcgtgttt atgcttaaat gcgtacttat atgcgtctat ttatgtagga tgaaaggtag
    12961 tctagtacct cctgtgatat tatcccattc catgcggggt atcgtatgct tccttcagca
    13021 ctacccttta gctgttctat atgctgccac tcctcaattg gattagtctc atccttcaat
    13081 gctatcattt cctttgatat tggatcatac taagaaacca ttattatcat gacattaacc
    13141 tataaaaata ggcgtatcac gaggcccttt cgtctcgcgc gtttcggtga tgacggtgaa
    13201 aacctcggcg cgccattacc ctgttatccc taatatacaa cgcaaagaag ctggaaaatt
    13261 ggcgagggaa tcctgtttct gtctatccaa gaatgggcat gaggtggcaa ccgtcgtgct
    13321 agcgtacagg gtgcactttg taaccatttg ggacaccgga cactcgctgt tttcgaaatt
    13381 accctttaag cgcgggtatt gaaccaggct tatgcccaag atcgtagcaa gcatttaact
    13441 gtgggaatac tcaggtatcg taagatgcaa gagttcgaat ctcttagcaa ccattatttt
    13501 tttcctcaac ataacgagaa cacacagggg cgctatcgca cagaatcaaa ttcgatgact
    13561 ggaaattttt tgttaatttc agaggtcgcc tgacgcatat acctttttca actgaaaaat
    13621 tgggagaaaa aggaaaggtg agaggccgga accggctttt catatagaat agagaagcgt
    13681 tcatgactaa atgcttgcat cacaatactt gaagttgaca atattattta aggacctatt
    13741 gttttttcca ataggtggtt agcaatcgtc ttactttcta acttttctta ccttttacat
    13801 ttcagcaata tatatatata tttcaaggat ataccattct aatgtctgcc cctatgtctg
    13861 cccctaagaa gatcgtcgtt ttgccaggtg accacgttgg tcaagaaatc acagccgaag
    13921 ccattaaggt tcttaaagct atttctgatg ttcgttccaa tgtcaagttc gatttcgaaa
    13981 atcatttaat tggtggtgct gctatcgatg ctacaggtgt cccacttcca gatgaggcgc
    14041 tggaagcctc caagaaggtt gatgccgttt tgttaggtgc tgtggctggt cctaaatggg
    14101 gtaccggtag tgttagacct gaacaaggtt tactaaaaat ccgtaaagaa cttcaattgt
    14161 acgccaactt aagaccatgt aactttgcat ccgactctct tttagactta tctccaatca
    14221 agccacaatt tgctaaaggt actgacttcg ttgttgtcag agaattagtg ggaggtattt
    14281 actttggtaa gagaaaggaa gacgatggtg atggtgtcgc ttgggatagt gaacaataca
    14341 ccgttccaga agtgcaaaga atcacaagaa tggccgcttt catggcccta caacatgagc
    14401 caccattgcc tatttggtcc ttggataaag ctaatctttt ggcctcttca agattatgga
    14461 gaaaaactgt ggaggaaacc atcaagaacg aattccctac attgaaggtt caacatcaat
    14521 tgattgattc tgccgccatg atcctagtta agaacccaac ccacctaaat ggtattataa
    14581 tcaccagcaa catgtttggt gatatcatct ccgatgaagc ctccgttatc ccaggttcct
    14641 tgggtttgtt gccatctgcg tccttggcct ctttgccaga caagaacacc gcatttggtt
    14701 tgtacgaacc atgccacggt tctgctccag atttgccaaa gaataaggtt gaccctatcg
    14761 ccactatctt gtctgctgca atgatgttga aattgtcatt gaacttgcct gaagaaggta
    14821 aggccattga agatgcagtt aaaaaggttt tggatgcagg tatcagaact ggtgatttag
    14881 gtggttccaa cagtaccacc gaagtcggtg atgctgtcgc cgaagaagtt aagaaaatcc
    14941 ttgcttaaaa agattctctt tttttatgat atttgtacat aaactttata aatgaaattc
    15001 ataatagaaa cgacacgaaa ttacaaaatg gaatatgttc atagggtaga cgaaactata
    15061 tacgcaatct acatacattt atcaagaagg agaaaaagga ggatagtaaa ggaatacagg
    15121 taagcaaatt gatactaatg gctcaacgaa atgacggtat cgataagctt gatatcgaat
    15181 tcctgcagcc cgggggatcc actagttcta gagttaatta a
//
