LOCUS       sequence-208189-       14210 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of a fungal biosynthetic gene cluster.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 14210)
  AUTHORS   Harvey CJB, Tang M, Schlecht U, Horecka J, Fischer CR, Lin HC, Li J,
            Naughton B, Cherry J, Miranda M, Li YF, Chu AM, Hennessy JR, Vandova
            GA, Inglis D, Aiyar RS, Steinmetz LM, Davis RW, Medema MH, Sattely 
            E, Khosla C, St Onge RP, Tang Y, Hillenmeyer ME
  TITLE     HEx: A heterologous expression platform for the discovery of fungal 
            natural products.
  JOURNAL   Sci Adv. 2018 Apr 11;4(4):eaar5459. doi: 10.1126/sciadv.aar5459. 
            eCollection 2018 Apr.
  PUBMED    29651464
REFERENCE   2  (bases 1 to 14210)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Adv."; 
            date: "2018-04-11"; volume: "4(4):eaar5459. doi"; pages: " 
            10.1126/sciadv.aar5459. eCollection 2018 Apr"
COMMENT     Sequence Label: pCHKU33.1-2.2
FEATURES             Location/Qualifiers
     source          1..14210
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             504..527
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     regulatory      2686..2695
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(8645..8665)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(8645..8663)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     rep_origin      complement(9002..9590)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(9082..9101)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9761..10552,10553..10621))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     10384..10403
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(10622..10726)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      10753..12095
                     /label=2u ori
                     /note="yeast 2u plasmid origin of replication"
     protein_bind    complement(11716..11763)
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     primer_bind     12136..12154
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        12426..12830
                     /gene="S. cerevisiae LEU2"
                     /label=LEU2 promoter
     CDS             12843..13937
                     /codon_start=1
                     /gene="S. cerevisiae LEU2"
                     /product="3-isopropylmalate dehydrogenase, required for 
                     leucine biosynthesis"
                     /label=LEU2
                     /note="yeast auxotrophic marker"
                     /translation="MSAPKKIVVLPGDHVGQEITAEAIKVLKAISDVRSNVKFDFENHL
                     IGGAAIDATGVPLPDEALEASKKVDAVLLGAVAGPKWGTGSVRPEQGLLKIRKELQLYA
                     NLRPCNFASDSLLDLSPIKPQFAKGTDFVVVRELVGGIYFGKRKEDDGDGVAWDSEQYT
                     VPEVQRITRMAAFMALQHEPPLPIWSLDKANLLASSRLWRKTVEETIKNEFPTLKVQHQ
                     LIDSAAMILVKNPTHLNGIIITSNMFGDIISDEASVIPGSLGLLPSASLASLPDKNTAF
                     GLYEPCHGSAPDLPKNKVDPIATILSAAMMLKLSLNLPEEGKAIEDAVKKVLDAGIRTG
                     DLGGSNSTTEVGDAVAEEVKKILA"
     primer_bind     complement(14185..14201)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(14185..14201)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
ORIGIN
        1 tatctaaaaa ttgccttatg atccgtctct ccggttacag cctgtgtaac tgattaatcc
       61 tgcctttcta atcaccattc taatgtttta attaagggat tttgtcttca ttaacggctt
      121 tcgctcataa aaatgttatg acgttttgcc cgcaggcggg aaaccatcca cttcacgaga
      181 ctgatctcct ctgccggaac accgggcatc tccaacttat aagttggaga aataagagaa
      241 tttcagattg agagaatgaa aaaaaaaaaa aaaaaaaagg cagaggagag catagaaatg
      301 gggttcactt tttggtaaag ctatagcatg cctatcacat ataaatagag tgccagtagc
      361 gacttttttc acactcgaaa tactcttact actgctctct tgttgttttt atcacttctt
      421 gtttcttctt ggtaaataga atatcaagct acaaaaagca tacaatcaac tatcaactat
      481 taactatatc gtaatacaca atggattata aggatgatga tgataagact agtatgggtt
      541 ctttagctgc taccgtcggg aatggggaaa ccaacgaatc atcccttcaa caccctgcaa
      601 ctcatggtag ccataccaac aacggccata cgatcaatgg ccatacaacc aacggtcata
      661 gcctcaatgg ccatttcata aacggccaca ccgcaaacga taacggtact atcaatggct
      721 cgctaaacgg gctcggctac ccgggtcaaa ctcccatggc catctgtggc atggcttgcc
      781 gcctcccagg aggaatagca tcggctgagg agttatggga attccttttg gcaaaaaaga
      841 atgcccggac tcgtgtgcct gaatcccgct acaacgtctc cgccttccac tcccccgttt
      901 ccaaaccagg aacggtggct acagaatacg ggcattttct tgatgatagc gtcgaccttg
      961 gggcactgga cacatctgtc ttccccttga atcgcactga gctagaaaga tcggacccgc
     1021 aacagcggct gatgttggag gttgccagag aggcctttga agatgccggg gtgaccgact
     1081 ggagggggaa gacggtggga tgctacatcg gaaactatgg agaggactgg tctgagatgt
     1141 ttgccaagga accgcagcaa tacggcttgc atcgaatcac tggtactggg gactttgtca
     1201 tcgcgaatcg gctatcatac gaattcgata tcaggggtcc cagcgtcaca atccgtacgg
     1261 cttgttcttc tgccctcatc ggcatcaacg aggcctgcgc ggccatcgcc cgtggagatt
     1321 gcgaagccgc catcgtgggc ggccttaatc tgatcctggc gccggccatg accatttctg
     1381 aaacagagca aggcgtttta tccaaggatg gctcttgcaa gaccttctct gcagatgcta
     1441 acggctatgc ccgtggagaa gctgttactg ctgtcttcat taaaccattg accgacgcaa
     1501 ttcgtgacgg caacccggtc cgggctgtca tccgggccgt aacgaataac tcggacggca
     1561 agacgcccgg gatatcacat ccaagcacta gctcgcagga aaccctcatg agaagagcgt
     1621 acgccatggc aggaatcacc gactttgggg aaacagctat ggttgagtgc cacggcaccg
     1681 gcacgcccat cggcgatcct attgaggcca atgccgtggc tcgtgttttt ggagagcggg
     1741 gagtttacat tggttctgtc aagccaaatt taggccatac agagggcgcc tcgggtcttg
     1801 tgtccctgat caagatggtt aaagcccttg agcaccgcac aataccaccc cagatccggt
     1861 tcacggctcc caatcccaac atccccttta aggaggccaa actcacggtt cccctggagc
     1921 caactccctg gcctcaagat cgcctcgagc gtgtaagtct gaactccttc ggcgtgggtg
     1981 gctccaacgc ccacattatc ctcgagtctg ccgccatgca caacgccgca accgtcattc
     2041 acgccacgcc ggaagaacct caactccttt tatacacggc cagttctgcc aagtccctca
     2101 ccagggtgat tgacaactac cggcactggg ttgagaggaa cccctccaag gtcgccgacc
     2161 tggcctacac tctggctagg aagcgagatc atgggccgca cagggccttc gctattgcca
     2221 acggcggcgc aattgaaacc gtctcgccgc ccgcaagcat caagcccggc acgaagccgc
     2281 ccaatatcgt catggtcttt acaggtcagg gcgcgcagtg gccccagatg ggtcgacagc
     2341 tatggcagtc caacgagacg ttcaaggcca gcatccagca cctggatcag tgtctccaag
     2401 ccatttctgg agaaaggcca cactactcaa tccaggagga gctcaagaag ccggccaaga
     2461 agagccgcgt atcttcggcc gagatctccc agcccctgtg cacggcaatt cagatcgcct
     2521 tggtcgactc cctcaaggcc ctgggcatcg tgccgcgtgc cgttgtcggc cattccagcg
     2581 gcgagattgc ggccgcctac gcctccgggg cgctcacagc gagtgaagcc atcgtggcag
     2641 ctcatcaccg tggcgccgtg acgaataggc agaagagaaa agggtccatg gcggccattg
     2701 gaatgagttg gcgagatacg gagaagcacc tcgtgcccaa cgtcgtcatt gcctgtgaca
     2761 actctcccaa aagtgtgaca atttcgggtg acaccgacaa ggtcaaggct gtggtggccg
     2821 atattcaaat gacacgtcca gaagtgttgg ctcggctgct tcaggtagac aaagcatacc
     2881 actcgtatca catggctgag attggagacg actacgcctc gttgattagc ggccaagtga
     2941 ctggaaagag tccttcgaat gccctcttct tctctagtgt gacgggaaag ctgctggatg
     3001 gagagcacaa tctcgggtcg gcatattggc gggcaaacct cgaatccccc gtgcggttcc
     3061 gtgaggccgt cacaaacctc ctgaatcgcg aggttggcaa gcatgccatc tttatcgaga
     3121 ttggccctca ctcggccctc gcgggaccgc taagacaaat attcgccgaa acctcttcac
     3181 cttgtcctta cctgtcagtc atgatccgca accaggattg tcttggatcc tatctggctg
     3241 ccgtcggcaa actccattca ctcaatgtaa acatcaacct cgacgtcttg ttccccacgg
     3301 gttcttgtct accagatctc ccaaggtacc cgtggaaaca cgagaacaca tgctggtacg
     3361 agtctcgtct gtccaaagat tggcgcttcc gaaagttccc ctatcatgat ctccttggcc
     3421 tccgagtcgt cgagagcacc gagctcgagc cggcctggcg caacctgttt cacctatcca
     3481 acacgccttg ggtccacgac cacaagatcg gggacgatat cgttttcccc tttgccggct
     3541 acattgccat ggctggcgag gcggtgaggc agacgacggg catcaacgac gggttcagca
     3601 ttcaaaatgt tcttgtcagc acagcactgg tcctccccaa gggcaggccc acggagatga
     3661 tgaccacgtt ccggccaaag agcctcacaa cgtccctgaa cagctcctgg tgggaattca
     3721 ccatggcctc gtacaacggt gacatctgga caaaacactg cacgggcgaa gtcatggcgc
     3781 tcatgtccga cccaagaccg ggacaggatc ccgaacccct tcctcgcaag gtgaacacgg
     3841 acaagtggta cagcaacatg agcaagggcg gtcttgacct tggcccctgt ttccaaacac
     3901 ttgccaccat tgagacttca acgagtccag aggacaacag ggccattgga aatgttgtca
     3961 acggcaggca gggcgatgag gccaactacc acatccatcc caccgtgctc gacgcgacca
     4021 tccagatcct aggtgccgcc gccaccaacg gccagatgcg caagaccaag acttggcttc
     4081 ctaccagcat ggacaggctc agcgtctacc gttgctcgtc ggacatggtc accagtgtca
     4141 gcgcgaggct gtcaagcaac cattccgtag tgggaggagg ccgttgcacg gcgggcggcg
     4201 tcgtcgtcgt cgaggctgag aatatcaaaa tgtcacttgc ggccaactcc ctttcaaatg
     4261 aggtccccga cacacacgcg gccgcaaggt acgagtggag cgccgatatt gactttatga
     4321 atgttggcga gctcctcctc gcaccagcag gtcgctctga atacttgcat ctcctcgagg
     4381 agctcgggca actctgcttg ctttcctcga agcgccgact ttactcgacg tctaagccgg
     4441 tggtcctcgc tcctcatatg ctcaactatg cgtcctggat tacgtccgct tccaaggtgg
     4501 tcgtcgtcaa cctagcgtct gtcatgagca atcttgacaa cgagaacatc tcggcccgca
     4561 tccacgctct ctcgaaccgt cttgctgata ctccaatgtc gccggtcgct aatgccatcc
     4621 gcgagatatg gacgaagatg gatctcttgc tgtccggaca gacgctggaa gatattctcc
     4681 cggaggagac actgtctagt gtcttgcagt tcgtgcacaa atccattgac tatggcccat
     4741 ttattcggca tctggcacac tccaaaccca acctgcgcat cctcgagatt ggcggcgaca
     4801 gaagctcatc ggtagccagt ggcattgccg aggacctcac acgccccgat ggccaggtgc
     4861 tctgttccaa gtataccttg agctcgccag gctttgtctc cagcaaggac cagccgatgc
     4921 tgctccccaa tatggaatac accactctgg atatcggcca agatcccgcg gatcagggct
     4981 ttgagggaca ccaatacgac ctcatcatcg catccaacgt cattggggcc accaggaacc
     5041 cgcagcagag cctggccaac atcaagagac tcttgagtcc taacggccga ctgcttatgc
     5101 aggagacttg cccgtcgtcc aaatgggcca cctatgtttt cggagtcctc ccgacgtggt
     5161 ggcaaggcac cgctgacggg tctgacgaag cagccgtcct cagtcccgaa atatggcagt
     5221 cgaagcttgc ggcggcaggg ctcggcagca tcgaagcaac ggttctggat gccgagcagc
     5281 ctcaccaact cactgcgacc atagtagcaa gacatgactt gccccaggag actgccccta
     5341 gacgagtcac tgttcttgtc gaagacgaga atggagctgc cacccggcgg atgctcagcc
     5401 gactagaaga tgacggttac gccgtgacaa ggtgcaagtt gaatggctct ccgccagtcg
     5461 gacaggatgt cctctcctgc ctggacctcg acaaaccctt ctttgagaat ctcagcgagc
     5521 cacgatatca gctcctcaaa gacttcctcc tcggcctcgg cggcgccggc gttctctgga
     5581 tcacggctct cagccagcgg ggatgccgtg atccccggta cgcccaagtc ttgggactcg
     5641 ctcgatgtgt ccgctccgag atgattgccg actttgccac gtgcgaggtg gacagcttcg
     5701 acaatgccca gtcgatggac atgatccccc gggtcctggc caaattcgag aagcgcgacg
     5761 acgacgccga cgacgccgta aagtccgact ttgaatgggc cattatcgac ggccaggtac
     5821 aggttgggcg cttccacaaa ttcgtgctgc gagacgaact ccttctgtcg gaacccgctg
     5881 gaatggcctc ccttgatatc gggacgcctg gcctagtcaa cagtctgcgt tgggtgcaac
     5941 agccgcgcga ggccgagctg aagccgggcg atgtagaggt ccaggtgcac tctgtaggct
     6001 tgaactttcg ggacatcctc gtggcccttg gcatcgtgga acttccggtg cgccagtttg
     6061 gattcgaagc agcaggcatc attacacggg tcggaaagga cgtggacccc aaccagctca
     6121 aggtcggcga tcgcatctgc tgcttgaaga agcaggcctt tgggacttat attgtcactc
     6181 cggactatag ttgtactcat gtccccgaca acatcacatt tgatgaggcc gcgtccatga
     6241 tcatgccata tattacggct atccacggct tgttcactac tgcacatctt tcaaagggtc
     6301 agaccgtctt gattcacagc gcatgcggtg gcgttggtct ggccgctgtg caaatttccc
     6361 aaatggtcgg cgctgaagtt tactgttccg tcggcagaga agacaaggtc gagttcctga
     6421 tgaacaactt caacatcccg cggaatcgca ttttctactc ccgtgacaag tcctttgttg
     6481 gtgacgttat gcgtgagact ggtggccaag gcatggacgt catcctcaac tccctttccg
     6541 gcgagctctt gcacgcaacg tggacatgcg tcgccgagtt tggcaccttg gttgaaattg
     6601 gaaagcgaga tctgatcggc cgtgggaaac tcgacatgga gccctttttg aagaaccgca
     6661 gctactgctg tattgacatt gaccgcgtct ggtcgaagcc gtggattctg agaaaacaca
     6721 tgctggacgc cttcgactgg tgtgccaagg gtcttgtcac ccccgttcga cccgtcaaat
     6781 ctttcccagc cagtcaaacc caggacgcgt tccggtacat gcaaaagggg tttcacattg
     6841 gacgaattgg catttccatc tctcacaacc cagaacaccc ggatacccag tttgagactg
     6901 tcaagatggc ccgacccatc acatttgaca gatccggttc ctaccttctt gttggtgggc
     6961 tcgggggtct aggccgcgcc atttccgtct ggatggccga gcacggcgcc cgcgaattga
     7021 tctacctatc acgaagcgct ggaggggaca ccaagcacga cgtcttcgtg gccgagctca
     7081 agagtatggg ctgcaacgtc aagctcatcc agggagacgt gaccaagctc gaggacgtcg
     7141 agaaggctgt caaggcggcg acacatgccc ttcgaggcat tatccaaatg tccatggtgc
     7201 tgcgtgacca aaactttacc aaaatgacct ttgaggagtg gacgggtgcg acagccccca
     7261 aggtcgaggg cacatggaac ctacaccgtg cctccctgtc cgctggtaca gacctggact
     7321 tttttgtgct cttcagctcc gtttcgggaa tcatcggcca accaggccag gccaactatg
     7381 ccagcgccaa cacctttctt gatgcgtttg cccagtaccg caagggcttg ggcctggcag
     7441 cctcggtcgt cgacgtcggg gccgttgaag acattggcgt cattaccgag cgccaaggat
     7501 taatgaacac catgaaagca accggcttca agggcgtcac agagcaggaa ctcttggacg
     7561 ccatggtctt ggcaatgttt gcccatgctc ggccgacggg gcagggaaat agtccaggct
     7621 tccactcggc ctttgtcgag gccaacacgt ttgtcctcgg cctggactcg tcgataccgc
     7681 tgagtagtcc caacaaccgc gccgtgtgga aaagggaccg tcgcatggcg gcgtaccaca
     7741 acgccgctgc caccggtgct gaagctgccg cgtccaacga tggtcttaag tcgttcctgg
     7801 ctggtgccag ggccgaccct gctgtcctca gctcgccgga gacggctaac ctgttcgccg
     7861 ttgagattgg caagaaactc tttcacctac tgctcaagcc acaggaggac ctcaacatag
     7921 ccttgccctt ggtcgacctg ggcctcgact cgctcgtggc actcgagctc aaggcctggt
     7981 ggaagcaggt cttctccttt gacatcagtg tgctggagat gcttgcgatg ggatcccttg
     8041 cggcgcttgg gaaacatgcc gccgatcaga tgatcaagat taccgccatt gataaggacg
     8101 cgtcctgagg agattgataa gacttttcta gttgcatatc ttttatattt aaatcttatc
     8161 tattagttaa ttttttgtaa tttatcctta tatatagtct ggttattcta aaatatcatt
     8221 tcagtatcta aaaattcccc tcttttttca gttatatctt aacaggcgac agtccaaatg
     8281 ttgatttatc ccagtccgat tcatcagggt tgtgaagcat tttgtcaatg gtcgaaatca
     8341 catcagtaat agtgcctctt acttgcctca tagaatttct ttctcttaac gtcaccgttt
     8401 ggtcttttat agtttcgaaa tctatggtga taccaaatgg tgttcccaat tcatcgttac
     8461 gggcgtattt tttaccaatt gaagtattgg aatcgtcaat tttaaagtat atctctcttt
     8521 tacgtaaagc ctgcgagatc ctcttaagta tagcggggaa gccatcgtta ttcgatattg
     8581 tcgtaacaaa tactttgatc ggcgctatgc ggccgccacc gcggtggagc tccagctttt
     8641 gttcccttta gtgagggtta attgcgcgct tggcgagact caaacaagat atattttgcc
     8701 cgccttacag acgaaactag ttggaggtta tggagcatac tatcacgtgg gcggccactg
     8761 gtgagttact acaccccagg ggcaacgttg atgctcctaa aaaactctgg ctggacgcaa
     8821 gccgtaacac ccgtgtcact tcataatcgt ttgcaattca gggcttgatc tacacattac
     8881 cctgttatcc ctattaatta atacggttat ccacagaatc aggggataac gcaggaaaga
     8941 acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt
     9001 ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt
     9061 ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc
     9121 gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa
     9181 gcgtggcgct ttctcatagc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct
     9241 ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta
     9301 actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg
     9361 gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc
     9421 ctaactacgg ctacactaga aggacagtat ttggtatctg cgctctgctg aagccagtta
     9481 ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg
     9541 gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt
     9601 tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg
     9661 tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa tgaagtttta
     9721 aatcaatcta aagtatatat gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg
     9781 aggcacctat ctcagcgatc tgtctatttc gttcatccat agttgcctga ctccccgtcg
     9841 tgtagataac tacgatacgg gagggcttac catctggccc cagtgctgca atgataccgc
     9901 gagacccacg ctcaccggct ccagatttat cagcaataaa ccagccagcc ggaagggccg
     9961 agcgcagaag tggtcctgca actttatccg cctccatcca gtctattaat tgttgccggg
    10021 aagctagagt aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc attgctacag
    10081 gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt cagctccggt tcccaacgat
    10141 caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc
    10201 cgatcgttgt cagaagtaag ttggccgcag tgttatcact catggttatg gcagcactgc
    10261 ataattctct tactgtcatg ccatccgtaa gatgcttttc tgtgactggt gagtactcaa
    10321 ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac
    10381 gggataatac cgcgccacat agcagaactt taaaagtgct catcattgga aaacgttctt
    10441 cggggcgaaa actctcaagg atcttaccgc tgttgagatc cagttcgatg taacccactc
    10501 gtgcacccaa ctgatcttca gcatctttta ctttcaccag cgtttctggg tgagcaaaaa
    10561 caggaaggca aaatgccgca aaaaagggaa taagggcgac acggaaatgt tgaatactca
    10621 tactcttcct ttttcaatat tattgaagca tttatcaggg ttattgtctc atgagcggat
    10681 acatatttga atgtatttag aaaaataaac aaataggggt tccgcgcaca tttccccgaa
    10741 aagtgccacc tgaacgaagc atctgtgctt cattttgtag aacaaaaatg caacgcgaga
    10801 gcgctaattt ttcaaacaaa gaatctgagc tgcattttta cagaacagaa atgcaacgcg
    10861 aaagcgctat tttaccaacg aagaatctgt gcttcatttt tgtaaaacaa aaatgcaacg
    10921 cgagagcgct aatttttcaa acaaagaatc tgagctgcat ttttacagaa cagaaatgca
    10981 acgcgagagc gctattttac caacaaagaa tctatacttc ttttttgttc tacaaaaatg
    11041 catcccgaga gcgctatttt tctaacaaag catcttagat tacttttttt ctcctttgtg
    11101 cgctctataa tgcagtctct tgataacttt ttgcactgta ggtccgttaa ggttagaaga
    11161 aggctacttt ggtgtctatt ttctcttcca taaaaaaagc ctgactccac ttcccgcgtt
    11221 tactgattac tagcgaagct gcgggtgcat tttttcaaga taaaggcatc cccgattata
    11281 ttctataccg atgtggattg cgcatacttt gtgaacagaa agtgatagcg ttgatgattc
    11341 ttcattggtc agaaaattat gaacggtttc ttctattttg tctctatata ctacgtatag
    11401 gaaatgttta cattttcgta ttgttttcga ttcactctat gaatagttct tactacaatt
    11461 tttttgtcta aagagtaata ctagagataa acataaaaaa tgtagaggtc gagtttagat
    11521 gcaagttcaa ggagcgaaag gtggatgggt aggttatata gggatatagc acagagatat
    11581 atagcaaaga gatacttttg agcaatgttt gtggaagcgg tattcgcaat attttagtag
    11641 ctcgttacag tccggtgcgt ttttggtttt ttgaaagtgc gtcttcagag cgcttttggt
    11701 tttcaaaagc gctctgaagt tcctatactt tctagagaat aggaacttcg gaataggaac
    11761 ttcaaagcgt ttccgaaaac gagcgcttcc gaaaatgcaa cgcgagctgc gcacatacag
    11821 ctcactgttc acgtcgcacc tatatctgcg tgttgcctgt atatatatat acatgagaag
    11881 aacggcatag tgcgtgttta tgcttaaatg cgtacttata tgcgtctatt tatgtaggat
    11941 gaaaggtagt ctagtacctc ctgtgatatt atcccattcc atgcggggta tcgtatgctt
    12001 ccttcagcac taccctttag ctgttctata tgctgccact cctcaattgg attagtctca
    12061 tccttcaatg ctatcatttc ctttgatatt ggatcatact aagaaaccat tattatcatg
    12121 acattaacct ataaaaatag gcgtatcacg aggccctttc gtctcgcgcg tttcggtgat
    12181 gacggtgaaa acctcggcgc gccattaccc tgttatccct aatatacaac gcaaagaagc
    12241 tggaaaattg gcgagggaat cctgtttctg tctatccaag aatgggcatg aggtggcaac
    12301 cgtcgtgcta gcgtacaggg tgcactttgt aaccatttgg gacaccggac actcgctgtt
    12361 ttcgaaatta ccctttaagc gcgggtattg aaccaggctt atgcccaaga tcgtagcaag
    12421 catttaactg tgggaatact caggtatcgt aagatgcaag agttcgaatc tcttagcaac
    12481 cattattttt ttcctcaaca taacgagaac acacaggggc gctatcgcac agaatcaaat
    12541 tcgatgactg gaaatttttt gttaatttca gaggtcgcct gacgcatata cctttttcaa
    12601 ctgaaaaatt gggagaaaaa ggaaaggtga gaggccggaa ccggcttttc atatagaata
    12661 gagaagcgtt catgactaaa tgcttgcatc acaatacttg aagttgacaa tattatttaa
    12721 ggacctattg ttttttccaa taggtggtta gcaatcgtct tactttctaa cttttcttac
    12781 cttttacatt tcagcaatat atatatatat ttcaaggata taccattcta atgtctgccc
    12841 ctatgtctgc ccctaagaag atcgtcgttt tgccaggtga ccacgttggt caagaaatca
    12901 cagccgaagc cattaaggtt cttaaagcta tttctgatgt tcgttccaat gtcaagttcg
    12961 atttcgaaaa tcatttaatt ggtggtgctg ctatcgatgc tacaggtgtc ccacttccag
    13021 atgaggcgct ggaagcctcc aagaaggttg atgccgtttt gttaggtgct gtggctggtc
    13081 ctaaatgggg taccggtagt gttagacctg aacaaggttt actaaaaatc cgtaaagaac
    13141 ttcaattgta cgccaactta agaccatgta actttgcatc cgactctctt ttagacttat
    13201 ctccaatcaa gccacaattt gctaaaggta ctgacttcgt tgttgtcaga gaattagtgg
    13261 gaggtattta ctttggtaag agaaaggaag acgatggtga tggtgtcgct tgggatagtg
    13321 aacaatacac cgttccagaa gtgcaaagaa tcacaagaat ggccgctttc atggccctac
    13381 aacatgagcc accattgcct atttggtcct tggataaagc taatcttttg gcctcttcaa
    13441 gattatggag aaaaactgtg gaggaaacca tcaagaacga attccctaca ttgaaggttc
    13501 aacatcaatt gattgattct gccgccatga tcctagttaa gaacccaacc cacctaaatg
    13561 gtattataat caccagcaac atgtttggtg atatcatctc cgatgaagcc tccgttatcc
    13621 caggttcctt gggtttgttg ccatctgcgt ccttggcctc tttgccagac aagaacaccg
    13681 catttggttt gtacgaacca tgccacggtt ctgctccaga tttgccaaag aataaggttg
    13741 accctatcgc cactatcttg tctgctgcaa tgatgttgaa attgtcattg aacttgcctg
    13801 aagaaggtaa ggccattgaa gatgcagtta aaaaggtttt ggatgcaggt atcagaactg
    13861 gtgatttagg tggttccaac agtaccaccg aagtcggtga tgctgtcgcc gaagaagtta
    13921 agaaaatcct tgcttaaaaa gattctcttt ttttatgata tttgtacata aactttataa
    13981 atgaaattca taatagaaac gacacgaaat tacaaaatgg aatatgttca tagggtagac
    14041 gaaactatat acgcaatcta catacattta tcaagaagga gaaaaaggag gatagtaaag
    14101 gaatacaggt aagcaaattg atactaatgg ctcaacgaaa tgacggtatc gataagcttg
    14161 atatcgaatt cctgcagccc gggggatcca ctagttctag agttaattaa
//
