LOCUS       sequence-20819-e         658 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 658)
  AUTHORS   Alberti S, Gitler AD, Lindquist S
  TITLE     A suite of Gateway cloning vectors for high-throughput genetic 
            analysis in Saccharomyces cerevisiae.
  JOURNAL   Yeast. 2007 Oct . 24(10):913-9.
  PUBMED    17583893
REFERENCE   2  (bases 1 to 658)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Yeast. 2007
            Oct . 24(10):913-9."
COMMENT     Sequence Label: pAG423GAL-ECFP-ccdB
FEATURES             Location/Qualifiers
     source          1..658
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     212..233
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(291..307)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(291..307)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     primer_bind     complement(328..351)
                     /label=GAL1
                     /note="S. cerevisiae GAL1 promoter, forward primer"
ORIGIN
        1 tagccttcgg gcatggcgga cttgaagaag tcgtgctgct tcatgtggtc ggggtagcgg
       61 ctgaagcact gcacgcccca ggtcagggtg gtcacgaggg tgggccaggg cacgggcagc
      121 ttgccggtgg tgcagatgaa cttcagggtc agcttgccgt aggtggcatc gccctcgccc
      181 tcgccggaca cgctgaactt gtggccgttt acgtcgccgt ccagctcgac caggatgggc
      241 accaccccgg tgaacagctc ctcgcccttg ctcaccattt tgtcccgggg gatccactag
      301 ttctagaatc cggggttttt tctccttgac gttaaagtat agaggtatat taacaatttt
      361 ttgttgatac ttttattaca tttgaataag aagtaataca aaccgaaaat gttgaaagta
      421 ttagttaaag tttatgcagt ttttgcattt atatatctgt taatagatca aaaatcatcg
      481 cttcgctgat taattacccc agaaataagg ctaaaaaact aatcgcatta tcatcctatg
      541 gttgttaatt tgattcgttc atttgaaggt ttgtggggcc aggttactgc caatttttcc
      601 tcttcataac cataaaagct agtattgtaa aatctttatt gttcggagca gtgcggcg
//
