LOCUS       sequence-208190-       17120 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of a fungal biosynthetic gene cluster.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 17120)
  AUTHORS   Harvey CJB, Tang M, Schlecht U, Horecka J, Fischer CR, Lin HC, Li J,
            Naughton B, Cherry J, Miranda M, Li YF, Chu AM, Hennessy JR, Vandova
            GA, Inglis D, Aiyar RS, Steinmetz LM, Davis RW, Medema MH, Sattely 
            E, Khosla C, St Onge RP, Tang Y, Hillenmeyer ME
  TITLE     HEx: A heterologous expression platform for the discovery of fungal 
            natural products.
  JOURNAL   Sci Adv. 2018 Apr 11;4(4):eaar5459. doi: 10.1126/sciadv.aar5459. 
            eCollection 2018 Apr.
  PUBMED    29651464
REFERENCE   2  (bases 1 to 17120)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Adv."; 
            date: "2018-04-11"; volume: "4(4):eaar5459. doi"; pages: " 
            10.1126/sciadv.aar5459. eCollection 2018 Apr"
COMMENT     Sequence Label: pCHKU33.2-2.2
FEATURES             Location/Qualifiers
     source          1..17120
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      1668..1967
                     /label=ADH2 terminator
                     /note="transcription terminator for the S. cerevisiae 
                     alcohol dehydrogenase 2 (ADH2) gene"
     promoter        3994..4214
                     /gene="S. cerevisiae URA3"
                     /label=URA3 promoter
     CDS             4215..5018
                     /codon_start=1
                     /gene="S. cerevisiae URA3"
                     /product="orotidine-5'-phosphate decarboxylase, required 
                     for uracil biosynthesis"
                     /label=URA3
                     /note="yeast auxotrophic marker, counterselectable with 
                     5-fluoroorotic acid (5-FOA)"
                     /translation="MSKATYKERAATHPSPVAAKLFNIMHEKQTNLCASLDVRTTKELL
                     ELVEALGPKICLLKTHVDILTDFSMEGTVKPLKALSAKYNFLLFEDRKFADIGNTVKLQ
                     YSAGVYRIAEWADITNAHGVVGPGIVSGLKQAAEEVTKEPRGLLMLAELSCKGSLSTGE
                     YTKGTVDIAKSDKDFVIGFIAQRDMGGRDEGYDWLIMTPGVGLDDKGDALGQQYRTVDD
                     VVSTGSDIIIVGRGLFAKGRDAKVEGERYRKAGWEAYLRRCGQQN"
     terminator      10359..10546
                     /gene="S. cerevisiae ADH1"
                     /label=ADH1 terminator
                     /note="transcription terminator for the S. cerevisiae 
                     alcohol dehydrogenase 1 (ADH1) gene"
     primer_bind     complement(10414..10438)
                     /label=pHybLex-R
                     /note="ADH terminator, reverse primer"
     primer_bind     complement(13267..13287)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(13267..13285)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     rep_origin      complement(13624..14212)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(13704..13723)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(14383..15174,15175..15243))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     15006..15025
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(15244..15348)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      15375..16717
                     /label=2u ori
                     /note="yeast 2u plasmid origin of replication"
     protein_bind    complement(16338..16385)
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     primer_bind     16758..16776
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(17095..17111)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(17095..17111)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
ORIGIN
        1 tatctaaaaa ttgccttatg atccgtctct ccggttacag cctgtgtaac tgattaatcc
       61 tgcctttcta atcaccattc taatgtttta attaagggat tttgtcttca ttaacggctt
      121 tcgctcataa aaatgttatg acgttttgcc cgcaggcggg aaaccatcca cttcacgaga
      181 ctgatctcct ctgccggaac accgggcatc tccaacttat aagttggaga aataagagaa
      241 tttcagattg agagaatgaa aaaaaaaaaa aaaaaaaagg cagaggagag catagaaatg
      301 gggttcactt tttggtaaag ctatagcatg cctatcacat ataaatagag tgccagtagc
      361 gacttttttc acactcgaaa tactcttact actgctctct tgttgttttt atcacttctt
      421 gtttcttctt ggtaaataga atatcaagct acaaaaagca tacaatcaac tatcaactat
      481 taactatatc gtaatacaca atgttttcaa gtctcttgtt gctcaaccta ttgcctctgg
      541 ccatcgcggc tccagccaag cgagctgagc cggcaccact ccttgtccca cgaggtgaca
      601 ctatccccga caagtatatt gtcaagtaca aagagacatt tgatatgtca gctgccgaca
      661 tcaccatcaa ggaataccat gccaaggcgg agaaaacgta ctctcacgtc ttcaacggat
      721 tcgctggcgc gttgaacgcg acgtccattg agacgcttcg taatcatcct gctgttgatt
      781 ttattgaaaa cgatgccact gtcaagatta gcgcatttgt cgaacagccg ggtgcccctt
      841 ggggtctgtc tcgcatttcg caccgacagc gcggagcaag tagctacgcc tatgatgaca
      901 gtgccggtga aggcacatgc gcgtatgtta tcgatactgg agtcgaggct tcgcatccga
      961 aattcgaagg tcgagccgag tttactaggt cctttgttgc cggagagact tctgatcgca
     1021 acggtcatgg cacccacgtg gccggaacca ttggcagtaa agactttggc gtggctaaga
     1081 aaaccaaaat ccgtggaatc aaggttctga gtgaccaagg cagcggcgat tactccggca
     1141 ttcttgccgg tatggacttt gctatccaag actctcgaga acgaccctgc cccaagggcg
     1201 tcgtcgccaa catgagcttg ggaggcggct attcagctgc catcaatcag gctgctgcaa
     1261 agatgatcca gtctggtgta ttccttgctg ttgcggcagg aaacgacgcg aaggatgcct
     1321 cccaaacgtc ccctgcctct gagcctagtg tatgcactgt aggagcaacg gattcttctg
     1381 atagactttc ctccttttca aacttcgggc ctgctgttga cattcttgcc cctggaagcg
     1441 atatcctgtc aacttggata ggtggcgcta ctaaatctat ctctggtact tccatggcca
     1501 cgccacatat tgttggtctt ggtgcctact tggcttcttt ggaaggcttc cccggtgcac
     1561 aggctctttg cgaaagaatt cgctccctgg ctacaaaaaa tactattagc ggtgtcccag
     1621 gaggaactgt caatttgctt gctttcaacg ggaatccatc tggataagcg gatctcttat
     1681 gtctttacga tttatagttt tcattatcaa gtatgcctat attagtatat agcatcttta
     1741 gatgacagtg ttcgaagttt cacgaataaa agataatatt ctactttttg ctcccaccgc
     1801 gtttgctagc acgagtgaac accatccctc gcctgtgagt tgtacccatt cctctaaact
     1861 gtagacatgg tagcttcagc agtgttcgtt atgtacggca tcctccaaca aacagtcggt
     1921 tatagtttgt cctgctcctc tgaatcgtct ccctcgatat ttctcatttt ccttcgcatg
     1981 ccagcattga aatgatcgaa gttcaatgat gaaacggtaa ttcttctgtc atttactcat
     2041 ctcatctcat caagttatat aattctatac ggatgtaatt tttcactttt cgtcttgacg
     2101 tccaccctat aatttcaatt attgaaccct cacgatccag ttctccagtg acacagcctt
     2161 tatctggtca aacctttctt tctaatcacc tatgctgatg cttaattaag ggatttttgt
     2221 ctccatcaac ggcatgcgcc caaaaatgac gtttttttta acccatagac acgaaactac
     2281 ccattttcca ccggcctgac ctaccaccgg aacaacggcc atctccaact tgcaagttgg
     2341 ggaaattaag agcatcgcag gtttaatgga agaaaaaaaa aaggtacagc acagcgcaaa
     2401 tggagttagt tcccttatgt cacacactca cacacagtcg gtcagatcaa gcatactggg
     2461 tgcgtataaa tagagtggcc attgccaccc tgtttatctc aaaatctgtc ttgttagtgg
     2521 tcttctccct ttttcaggtt acaattctct tgtttctact tagtatataa gtatatcaag
     2581 ctatattaag catactatca actgtcaact ctatcctcaa aatacaatac aaaatgtggc
     2641 gcatcccaac ggacacccct caaacttcca ctcccaacgg acgcaccaag ctccaggaag
     2701 tcaccccaga aacccccttg gacgaaatct tccaaaagtg gggagaagat ggcgcccttg
     2761 tcatcaaagg cctcctcacc cctggtcaag tagccgaatt cacctctgaa atcgaacaag
     2821 tcattgaaaa aactcggcgt ggcgctctgc tggaccaccc gctccttcaa gcctttcacg
     2881 gccgccgaac gaagcgcgcc gccggtctcg ccaaccatag caaggtgttt cgcgagaacc
     2941 ttctcgagaa cgacctagtt catgccatat gccaccgcaa ctacactacg ggaggcaacg
     3001 ccggcgatta ctggctgtcc acggggtcaa ctttgtttgc cggcggtccg caggacccgc
     3061 aggagctgca tcgagacttg aacaactacc ctcctgccgt cttgcttggc ccagactttg
     3121 acgatgtgca aatgaacttt ctgattgccc tcagcccgtt cagggaggac aacggcgcga
     3181 cccgggtcat cccagggagt cagaagtggc cctttgacca gcgcggcaac caggaaatga
     3241 cgatcccggc catcatggac ccgggcgact gcctcctttt gagtggcaag gtcgtgcacg
     3301 gcatgggtgc gaatacaacc acggaggagc gaaaggcatt gcagctgtct gtttgcgcag
     3361 gctatctgac gccatccgag gcgcatccga gcattgtcac cctagagact gccaagaagc
     3421 tgtctccaag ggcgcagcga tttttgggtt tccggtccca gtaccctagg gggtcacctg
     3481 gattgtggac caaggattac actgagctgg ctttgcattt ggaactggat gatcttgatg
     3541 gcctgatggt ttatcttcgt gaccttgcga aaagtcatcc agttgaagac ccggttggca
     3601 aattgtttta gacaaatcgc tcttaaatat atacctaaag aacattaaag ctatattata
     3661 agcaaagata cgtaaatttt gcttatatta ttatacacat atcatatttc tatattttta
     3721 agatttggtt atataatgta cgtaatgcaa aggaaataaa ttttatacat tattgaacag
     3781 cgtccaagta actacattat gtgcactaat agtttagcgt cgtgaagact ttattgtgtc
     3841 gcgaaaagta aaaattttaa aaattagagc accttgaact tgcgaaaaag gttctcatca
     3901 actgtttaaa aggaggatat caggtcctat ttctgacaaa caatatacaa atttagtttc
     3961 aaagatgaat cagtgcgcga aggacataac tcattcaatt caattcatca tttttttttt
     4021 attctttttt ttgatttcgg tttctttgaa atttttttga ttcggtaatc tccgaacaga
     4081 aggaagaacg aaggaaggag cacagactta gattggtata tatacgcata tgtagtgttg
     4141 aagaaacatg aaattgccca gtattcttaa cccaactgca cagaacaaaa acctgcagga
     4201 aacgaagata aatcatgtcg aaagctacat ataaggaacg tgctgctact catcctagtc
     4261 ctgttgctgc caagctattt aatatcatgc acgaaaagca aacaaacttg tgtgcttcat
     4321 tggatgttcg taccaccaag gaattactgg agttagttga agcattaggt cccaaaattt
     4381 gtttactaaa aacacatgtg gatatcttga ctgatttttc catggagggc acagttaagc
     4441 cgctaaaggc attatccgcc aagtacaatt ttttactctt cgaagacaga aaatttgctg
     4501 acattggtaa tacagtcaaa ttgcagtact ctgcgggtgt atacagaata gcagaatggg
     4561 cagacattac gaatgcacac ggtgtggtgg gcccaggtat tgttagcggt ttgaagcagg
     4621 cggcagaaga agtaacaaag gaacctagag gccttttgat gttagcagaa ttgtcatgca
     4681 agggctccct atctactgga gaatatacta agggtactgt tgacattgcg aagagcgaca
     4741 aagattttgt tatcggcttt attgctcaaa gagacatggg tggaagagat gaaggttacg
     4801 attggttgat tatgacaccc ggtgtgggtt tagatgacaa gggagacgca ttgggtcaac
     4861 agtatagaac cgtggatgat gtggtctcta caggatctga cattattatt gttggaagag
     4921 gactatttgc aaagggaagg gatgctaagg tagagggtga acgttacaga aaagcaggct
     4981 gggaagcata tttgagaaga tgcggccagc aaaactaaaa aactgtatta taagtaaatg
     5041 catgtatact aaactcacaa attagagctt caatttaatt atatcagtta ttaccctatg
     5101 cggtgtgaaa taccgcacag atgcgtaagg agaaaatacc gcatcaggat aggaaaaaac
     5161 cgagcttcct ttcatccggc gcggctgtgt tctacatatc actgaagctc cgggtatttt
     5221 aagttataca agggaaagat gccggctaga ctagcaagtt ttaggctgct taacattatg
     5281 gataggcgga taaagggccc aaacaggatt gtaaagctta gacgcttctg gttggacaat
     5341 ggtacgtttg tgtattaagt aaggcttggc tggggatagc aacattgggc agagtataga
     5401 agaccacaaa aaaaaggtat ataagggcag agaagtcttt gtaatgtgtg taacttctct
     5461 tccatgtgta atcagtattt ctacttactt cttaaatata cagaagtaag acagataacc
     5521 aacagccttt cccagatata catatatatc tttatttcag cttaaacaat aattatattt
     5581 gtttaactca aaaataaaaa aaaaaaacca aactcacgca actaattatt ccataataaa
     5641 ataacaacat ggccaaatcc tatcatttgt ccttggatat tgctaaaggc atccctaaga
     5701 ctctccttct caaccgacaa ccctcaatct ctcacctgag agcaaaagaa ggacgtgtgg
     5761 atcactcgat gatgaagcga cccgaggatt ttcgcggaaa ggcaagtttt ctgctctgcg
     5821 gggataccgt ctttgttacc ggggggtctg ggggattggg caaagccata tcccagcagt
     5881 tagcagcccg aggggcccat atcacaattt tctcccgcag gccagacgca cttgccaggg
     5941 ccagagagga gatactttcc aagtgtttga ctggggatca ggaggtcaat accgtcgcca
     6001 tcgacatggc ggatgcttca aaggttgacg aagcatttag aaaacagcct cggattgctg
     6061 acgtcttgta ctgtgttgcc ggcggtaacc atgctgagaa cggatttctc gtagatatta
     6121 aggcacaggc tcttgaaagt tgcatgcgga acaactactt taccgccgtg tacgcagcca
     6181 agtctctgct cgacatatgg acagaggatg atttgaaagg acccattcat ccccgccctg
     6241 accccaggat acgtcagatt gtctttgtca caagcgccgc cgcgtttttg ggttctccgg
     6301 gctccatttc ttacactccg gccaagtgtg caacccgcgc ctttgcggat actctgcggt
     6361 tggaagtgct gcggtactgc tgccccgagt cgacctactc gatccactgc gcctttccag
     6421 gggactttgt atcccctgga tttgtcttcg aacagaagac caagacgaat ctcacaaagc
     6481 gcattcaggg tctcgacggc tacaccatgt cggagctcga agctcggttc ccatcttcgg
     6541 acaagatcgc ttcactcatc acttccgccg tggacagggg cgatttcatc atctgtgacg
     6601 gctcgctggc tggctcattg ctcttcacca acatgattgg gccgtcacca aagaggggat
     6661 tgggcattgt ggattctttg ctttctgtat ttaccgggtg tcttctctgg ccatatctca
     6721 ggtggaaatg ggagtccatg acccgcaagg acggcgaaga gcacagaaga gctaggtgaa
     6781 gtgcttttaa ctaagaatta ttagtctttt ctgcttattt tttcatcata gtttagaaca
     6841 ctttatatta acgaatagtt tatgaatcta tttaggttta aaaattgata cagttttata
     6901 agttactttt tcaaagactc gtgctgtcta ttgcataatg cactggaagg ggaaaaaaaa
     6961 ggtgcacacg cgtggctttt tcttgaattt gcagtttgaa aaataactac atggatgata
     7021 agaaaacatg gagtacagtc actttgagaa ccttcaatca gctggtaacg tcttcgttaa
     7081 ttggatactc aaaaaagatg gatagcatga atcacaagat ggaaggaaat gcgggccacg
     7141 accacagtga tatgcatatg ggagatggag atgatacctc cattgggccg atgaagttag
     7201 tcgacggata gaagcggttg tcccctttcc cggcgagccg gcagtcgggc cgaggttcgg
     7261 ataaattttg tattgtgttt tgattctgtc atgagtatta cttatgttct ctttaggtaa
     7321 ccccaggtta atcaatcaca gtttcatacc ggctagtatt caaattatga cttttcttct
     7381 gcagtgtcag ccttacgacg attatctatg agctttgaat atagtttgcc gtgattcgta
     7441 tctttaattg gataataaaa tgcgaaggat cgatgaccct tattattatt tttctacact
     7501 ggctaccgat ttaactcatc ttcttgaaag tatataagta acagtaaaat ataccgtact
     7561 tctgctaatg ttatttgtcc cttatttttc ttttcttgtc ttatgctata gtacctaaga
     7621 ataacgacta ttgttttgaa ctaaacaaag tagtaaaagc acataaaaga attaagaaaa
     7681 tggatttcct ggcggatttt atcaaggctc agaagatcaa ggggccccag atgaaagatg
     7741 ccccaacctt ctaccgcaac ctcgaggagg ctttggacct ccggcgatcc gatcatggca
     7801 tgttcaccaa cggtatgaag agtccctgga agtcaggcaa ggccgtcgac ttttgctcca
     7861 acgatcttct gtccctggga aagacgggac tgcttcgcaa ggagttcctc aacgagctgg
     7921 gccgtaactc cgacttcccc atctatgcag gaggttcgcg cctggtggac gggaactacg
     7981 attacattga acaagttgag caggaaattg ccgccttcca cggcgctgag atgtcactca
     8041 ttgtcggctc cggtttcgag gctaacaatg ccatctactc ggcaatccca cgccccggcg
     8101 acgccatagt ctacgacgag ctcgtccacg ccagcgtcca tgacggcatg acgcactcgc
     8161 tggccttgac caaggttgcc ttccgccata atgatgtcga cgccttgaga gatgcgctag
     8221 tagaacttgt cgactcacag cccttgatca aggacggctc gcgcacggtt cttatatccg
     8281 tcgagtccgt gtacagcatg gatggtgacg tttgccctct acgcgagatg cttgagattt
     8341 cccgcgaagt gtgccccaag atgaattttg cttttattgt cgacgaagcc catgctacgg
     8401 ggataattgg ccccaagggc gtaggacttg tcagtctctt aggtctcgag aaggagattg
     8461 ctattcgact ccatacatat ggaaaggctt tggcctcgac aggagccgcc attttgagca
     8521 acggaacggt tcgaacggct ctgctcaatt ttgcccgctc cgtcatctac accactggcc
     8581 cttcctttcc ttcggttgca tgtattcgag cagcctacaa cttgatgcgc aacggagaaa
     8641 cgacaaagtt acaaaacagc atccagcatc tcgtgaagca ctttttcgaa accatcacgt
     8701 cgaaccctgt gtgggaaaaa gccagcgaga tggggattct ggccatccct ctggtcgacg
     8761 attgggaagg acgcgagttc cagacgcaca ttgtacccct ctggacacgg cagcggtata
     8821 actggtggct cgtattccat ctgcagttgg cgggaattgc ggccgtcccc attgactacc
     8881 ccacggttcc aaagggccag agcaggattc ggatcatggt tcatagcgcc aacacggagg
     8941 ccgaggtcga ttttcttgcc agcaccatat gtgaatgggc acaagagatg attggcattg
     9001 aggagtcggg ggaggtcaag ggaaagacgc ccaaagccgc tcagaaaatc tacgccttga
     9061 tggctgctgc tagcgcttga atttaactcc ttaagttact ttaatgattt agtttttatt
     9121 attaataatt catgctcatg acatctcata tacacgttta taaaacttaa atagattgaa
     9181 aatgtattaa agattcctca gggattcgat ttttttggaa gtttttgttt ttttttcctt
     9241 gagatgctgt agtatttggg aacaattata caatcgaaag atatatgctt acattcgacc
     9301 gttttagccg tgatcattat cctatagtaa cataacctga agcataactg acactactat
     9361 catcaatact tgtcacatga gaactctgtg aataattagg ccactgaaat ttgatgcctg
     9421 aaggaccggc atcacggatt ttcgataaag cacttagtat cacactaatt ggcttttcgc
     9481 tagtcttatc taaaaattgc ctttatagtc cgtctctcca gtcacggcct gtgtaactga
     9541 ttaatcctgc ctttctaatc accattctac tgtttaatta agggattttg tcttcatcaa
     9601 cggcttccgc ccaaaaaaaa gtatgacgtt ttgcccgcag gcgtgaagct gcccatcttc
     9661 acgggcctga cctcctctgc cggaacaccg gccatctcca actcataaat tggagaaata
     9721 agagaatttc agattttcag aggatgaaaa aaaaaaggta gagagcataa aaatggggtt
     9781 cactttttgg caaagttaca gtatgcttat tacatataaa tagagtgccg ataatggctt
     9841 tttttcatct tcgaaatacg cttgctactg ctcttccagc gtttttatta cttctttctt
     9901 gtttctcctt agtatataaa atatcaagct acaacaagca tacaatcaac tgtcaactgt
     9961 caattatatt ataatacact atgctcggct ttgccaaaac ccttcttctt gctacccagc
    10021 tggcatattt tgccagtgcg tcgcccaccc tcaacaataa caataagctt acgagcaaat
    10081 ggcccgtgtg tatccctccc ccaacacaag tctcaaccaa gcgaacgccg actgagaagc
    10141 gagacgccag cgttacgact ccgttgccca cccagccagg catggttggc aactgcaaca
    10201 agttctgctg gatagagaat ggcgacatct gcgggcagat tgctgagagc aatgacattt
    10261 ccactaagca attcatccaa tggaacaaag gcgctggtcc ggattgcctc actctctggg
    10321 cgaaaacata tggttgcgtg ggcgttacgg actcgtaagc gaatttctta tgatttatga
    10381 tttttattat taaataagtt ataaaaaaaa taagtgtata caaattttaa agtgactctt
    10441 aggttttaaa acgaaaattc ttattcttga gtaactcttt cctgtaggtc aggttgcttt
    10501 ctcaggtata gcatgaggtc gctcttattg accacacctc taccggcatg cctctcaaat
    10561 cttttagcgc caaggactcc aactaattgt atcttgaatt tgcctttacg atccgtttgt
    10621 ccagtcacgg catgtatatc ttattaatcc tgcctttcta atcacgtatt ctaatgttca
    10681 attaagggat tttatcttca tcaacggctc ccacgcaaaa aatgacgttt tgcacacaga
    10741 cacgaaatac accttccacc ggaacaacgg ccatctccaa cttataagtt ggggaaataa
    10801 gacaatttca gacttcagag aatgaaaaaa aaaaaaggta catcacagat ggggttcagg
    10861 tttgctacaa ttgcagggag cctgtcacat ataaatagac ctccagtgat gatatctttc
    10921 agtcttcaaa cgtctcttgt cacagttctg gtcgttctat atcacatctc tcttggttct
    10981 acttattgtc tataatatca agctacagca agcatacaat caactatcta ccataccata
    11041 atacacaatg gatcaacaac cagaaccgga ccacggacaa aatatcaggc gaaggcgtag
    11101 tcggcagtcg tctgcggcgc cgtccgtcca gctagaagcg aatgcagctc ctgctttacc
    11161 tcaacgggca ggacggccgc tttccttttg gctcgccttt tccggtctat gtatcaacac
    11221 gtttctcttc gctctcgact cgtccactct ctccgtggct cttccagcca ttgcgcacga
    11281 gttgcatggc acaactttcg agtcgttctg ggccggaatc gcgtaccttc tttgcgtggt
    11341 catcacccag ccattgtaca cctcactgtc cgacgtcttc ggtcgcaagc ctctgctcta
    11401 tgccgctctc ctgctctttg ccatcggctc gctagtcttt ggtcttgcca agaacatgac
    11461 gtatatcatt gtcggccgag cgtttcaggg ctttgggggc gggggtatgg atctgctaag
    11521 cgagatcatc ataacggata taacgacgct gcaggagcgt gccatgtttc tcgggctgct
    11581 ggctattccc atcgccattg ggaccatcat cggtccaact atcggcgccg tcttcagcga
    11641 attcatctct tggcgatgga tcggctacgc aaacttgccc tttgtcggag tctcgttccc
    11701 catcacattc tttttccttc gtctccgtac ggtcaacgac tccctcatgg acgatgtgaa
    11761 gaggctggat tggggtggca tggctctctc cttcaccgga atgattcttt tcgtgctacc
    11821 tcttagttgg ggcggcgcct tgtactcgtg ggcctcatgg cagacacttg tcccgttgat
    11881 tatgggaatc cttattctgg ttggttactt tgcctacgag tcactacccg ccgctccgat
    11941 catgccctat cgattgctac gatctacaac agccaaggcg acactgctgg ggaactttat
    12001 gcaaggcttc gttctcttcc cgctgcaaca gtacttgttg ctcttgttcc aggcagtcta
    12061 cctactgagc caaatcgaca cggctgtgga gctgctccct accagcatca catgcgtctt
    12121 ggccgccagt ggtgtggtca tcaccatagg tctactcaaa tctggatatc tatggagtat
    12181 cagactgttc tgggttgtcc tcacagtggg caccggcctt gtcgccctgt tagatgaagg
    12241 ctccccccgt gctatgcgaa tggccatccc catcatctgg ggcgcgggta taggcgcatt
    12301 gttgcgtctc ctccaccttc caatgcaagc aagtgtcgaa aacgtggatg acacggggct
    12361 ggccgtcggg ctcgtccttt ggtttcgcct ggtgggaggc ctcctcggac tcgccgtctc
    12421 atcctcaata tttatcagtg tctttgccaa gtccattgcc ggcgttgaca acctccccga
    12481 gtctcttgct cccctgagag aagcgagccg ggccattagc tttataccaa cgctgaggga
    12541 tctgggtctt tcccgggacg tgttgggcct agtcttggga gcttaccaca aagctttcca
    12601 gacagtattt tacgcaatga ctgcctttgg cggtgttggt ttcgtaagct cactatttac
    12661 caaggagctc actctgcaga aaagcgacct tggccggcag gcgtttgagg gacagcaatt
    12721 attaccctaa ggagattgat aagacttttc tagttgcata tcttttatat ttaaatctta
    12781 tctattagtt aattttttgt aatttatcct tatatatagt ctggttattc taaaatatca
    12841 tttcagtatc taaaaattcc cctctttttt cagttatatc ttaacaggcg acagtccaaa
    12901 tgttgattta tcccagtccg attcatcagg gttgtgaagc attttgtcaa tggtcgaaat
    12961 cacatcagta atagtgcctc ttacttgcct catagaattt ctttctctta acgtcaccgt
    13021 ttggtctttt atagtttcga aatctatggt gataccaaat ggtgttccca attcatcgtt
    13081 acgggcgtat tttttaccaa ttgaagtatt ggaatcgtca attttaaagt atatctctct
    13141 tttacgtaaa gcctgcgaga tcctcttaag tatagcgggg aagccatcgt tattcgatat
    13201 tgtcgtaaca aatactttga tcggcgctat gcggccgcca ccgcggtgga gctccagctt
    13261 ttgttccctt tagtgagggt taattgcgcg cttggcgaga ctcaaacaag atatattttg
    13321 cccgccttac agacgaaact agttggaggt tatggagcat actatcacgt gggcggccac
    13381 tggtgagtta ctacacccca ggggcaacgt tgatgctcct aaaaaactct ggctggacgc
    13441 aagccgtaac acccgtgtca cttcataatc gtttgcaatt cagggcttga tctacacatt
    13501 accctgttat ccctattaat taatacggtt atccacagaa tcaggggata acgcaggaaa
    13561 gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc
    13621 gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag
    13681 gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt
    13741 gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg
    13801 aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt aggtcgttcg
    13861 ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg
    13921 taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac
    13981 tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg
    14041 gcctaactac ggctacacta gaaggacagt atttggtatc tgcgctctgc tgaagccagt
    14101 taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg
    14161 tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc
    14221 tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt
    14281 ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa aatgaagttt
    14341 taaatcaatc taaagtatat atgagtaaac ttggtctgac agttaccaat gcttaatcag
    14401 tgaggcacct atctcagcga tctgtctatt tcgttcatcc atagttgcct gactccccgt
    14461 cgtgtagata actacgatac gggagggctt accatctggc cccagtgctg caatgatacc
    14521 gcgagaccca cgctcaccgg ctccagattt atcagcaata aaccagccag ccggaagggc
    14581 cgagcgcaga agtggtcctg caactttatc cgcctccatc cagtctatta attgttgccg
    14641 ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg ccattgctac
    14701 aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg gttcccaacg
    14761 atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc
    14821 tccgatcgtt gtcagaagta agttggccgc agtgttatca ctcatggtta tggcagcact
    14881 gcataattct cttactgtca tgccatccgt aagatgcttt tctgtgactg gtgagtactc
    14941 aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat
    15001 acgggataat accgcgccac atagcagaac tttaaaagtg ctcatcattg gaaaacgttc
    15061 ttcggggcga aaactctcaa ggatcttacc gctgttgaga tccagttcga tgtaacccac
    15121 tcgtgcaccc aactgatctt cagcatcttt tactttcacc agcgtttctg ggtgagcaaa
    15181 aacaggaagg caaaatgccg caaaaaaggg aataagggcg acacggaaat gttgaatact
    15241 catactcttc ctttttcaat attattgaag catttatcag ggttattgtc tcatgagcgg
    15301 atacatattt gaatgtattt agaaaaataa acaaataggg gttccgcgca catttccccg
    15361 aaaagtgcca cctgaacgaa gcatctgtgc ttcattttgt agaacaaaaa tgcaacgcga
    15421 gagcgctaat ttttcaaaca aagaatctga gctgcatttt tacagaacag aaatgcaacg
    15481 cgaaagcgct attttaccaa cgaagaatct gtgcttcatt tttgtaaaac aaaaatgcaa
    15541 cgcgagagcg ctaatttttc aaacaaagaa tctgagctgc atttttacag aacagaaatg
    15601 caacgcgaga gcgctatttt accaacaaag aatctatact tcttttttgt tctacaaaaa
    15661 tgcatcccga gagcgctatt tttctaacaa agcatcttag attacttttt ttctcctttg
    15721 tgcgctctat aatgcagtct cttgataact ttttgcactg taggtccgtt aaggttagaa
    15781 gaaggctact ttggtgtcta ttttctcttc cataaaaaaa gcctgactcc acttcccgcg
    15841 tttactgatt actagcgaag ctgcgggtgc attttttcaa gataaaggca tccccgatta
    15901 tattctatac cgatgtggat tgcgcatact ttgtgaacag aaagtgatag cgttgatgat
    15961 tcttcattgg tcagaaaatt atgaacggtt tcttctattt tgtctctata tactacgtat
    16021 aggaaatgtt tacattttcg tattgttttc gattcactct atgaatagtt cttactacaa
    16081 tttttttgtc taaagagtaa tactagagat aaacataaaa aatgtagagg tcgagtttag
    16141 atgcaagttc aaggagcgaa aggtggatgg gtaggttata tagggatata gcacagagat
    16201 atatagcaaa gagatacttt tgagcaatgt ttgtggaagc ggtattcgca atattttagt
    16261 agctcgttac agtccggtgc gtttttggtt ttttgaaagt gcgtcttcag agcgcttttg
    16321 gttttcaaaa gcgctctgaa gttcctatac tttctagaga ataggaactt cggaatagga
    16381 acttcaaagc gtttccgaaa acgagcgctt ccgaaaatgc aacgcgagct gcgcacatac
    16441 agctcactgt tcacgtcgca cctatatctg cgtgttgcct gtatatatat atacatgaga
    16501 agaacggcat agtgcgtgtt tatgcttaaa tgcgtactta tatgcgtcta tttatgtagg
    16561 atgaaaggta gtctagtacc tcctgtgata ttatcccatt ccatgcgggg tatcgtatgc
    16621 ttccttcagc actacccttt agctgttcta tatgctgcca ctcctcaatt ggattagtct
    16681 catccttcaa tgctatcatt tcctttgata ttggatcata ctaagaaacc attattatca
    16741 tgacattaac ctataaaaat aggcgtatca cgaggccctt tcgtctcgcg cgtttcggtg
    16801 atgacggtga aaacctcggc gcgccattac cctgttatcc ctaatataca acgcaaagaa
    16861 gctggaaaat tggcgaggga atcctgtttc tgtctatcca agaatgggca tgaggtggca
    16921 accgtcgtgc tagcgtacag ggtgcacttt gtaaccattt gggacaccgg acactcgctg
    16981 ttttcgaaat taccctttaa gcgcgggtat tgaaccaggc ttatgcccaa gatcgtagca
    17041 agcatttaaa tgacggtatc gataagcttg atatcgaatt cctgcagccc gggggatcca
    17101 ctagttctag agttaattaa
//
