LOCUS       sequence-208192-       12977 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of a fungal biosynthetic gene cluster.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12977)
  AUTHORS   Harvey CJB, Tang M, Schlecht U, Horecka J, Fischer CR, Lin HC, Li J,
            Naughton B, Cherry J, Miranda M, Li YF, Chu AM, Hennessy JR, Vandova
            GA, Inglis D, Aiyar RS, Steinmetz LM, Davis RW, Medema MH, Sattely 
            E, Khosla C, St Onge RP, Tang Y, Hillenmeyer ME
  TITLE     HEx: A heterologous expression platform for the discovery of fungal 
            natural products.
  JOURNAL   Sci Adv. 2018 Apr 11;4(4):eaar5459. doi: 10.1126/sciadv.aar5459. 
            eCollection 2018 Apr.
  PUBMED    29651464
REFERENCE   2  (bases 1 to 12977)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Adv."; 
            date: "2018-04-11"; volume: "4(4):eaar5459. doi"; pages: " 
            10.1126/sciadv.aar5459. eCollection 2018 Apr"
COMMENT     Sequence Label: pCHKU37.1-2.2
FEATURES             Location/Qualifiers
     source          1..12977
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             504..527
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     primer_bind     complement(7412..7432)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(7412..7430)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     rep_origin      complement(7769..8357)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7849..7868)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8528..9319,9320..9388))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9151..9170
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9389..9493)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      9520..10862
                     /label=2u ori
                     /note="yeast 2u plasmid origin of replication"
     protein_bind    complement(10483..10530)
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     primer_bind     10903..10921
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        11193..11597
                     /gene="S. cerevisiae LEU2"
                     /label=LEU2 promoter
     CDS             11610..12704
                     /codon_start=1
                     /gene="S. cerevisiae LEU2"
                     /product="3-isopropylmalate dehydrogenase, required for 
                     leucine biosynthesis"
                     /label=LEU2
                     /note="yeast auxotrophic marker"
                     /translation="MSAPKKIVVLPGDHVGQEITAEAIKVLKAISDVRSNVKFDFENHL
                     IGGAAIDATGVPLPDEALEASKKVDAVLLGAVAGPKWGTGSVRPEQGLLKIRKELQLYA
                     NLRPCNFASDSLLDLSPIKPQFAKGTDFVVVRELVGGIYFGKRKEDDGDGVAWDSEQYT
                     VPEVQRITRMAAFMALQHEPPLPIWSLDKANLLASSRLWRKTVEETIKNEFPTLKVQHQ
                     LIDSAAMILVKNPTHLNGIIITSNMFGDIISDEASVIPGSLGLLPSASLASLPDKNTAF
                     GLYEPCHGSAPDLPKNKVDPIATILSAAMMLKLSLNLPEEGKAIEDAVKKVLDAGIRTG
                     DLGGSNSTTEVGDAVAEEVKKILA"
     primer_bind     complement(12952..12968)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(12952..12968)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
ORIGIN
        1 tatctaaaaa ttgccttatg atccgtctct ccggttacag cctgtgtaac tgattaatcc
       61 tgcctttcta atcaccattc taatgtttta attaagggat tttgtcttca ttaacggctt
      121 tcgctcataa aaatgttatg acgttttgcc cgcaggcggg aaaccatcca cttcacgaga
      181 ctgatctcct ctgccggaac accgggcatc tccaacttat aagttggaga aataagagaa
      241 tttcagattg agagaatgaa aaaaaaaaaa aaaaaaaagg cagaggagag catagaaatg
      301 gggttcactt tttggtaaag ctatagcatg cctatcacat ataaatagag tgccagtagc
      361 gacttttttc acactcgaaa tactcttact actgctctct tgttgttttt atcacttctt
      421 gtttcttctt ggtaaataga atatcaagct acaaaaagca tacaatcaac tatcaactat
      481 taactatatc gtaatacaca atggattata aggatgatga tgataagact agtatgcagc
      541 cgcaaacaga aattatggct tcttgccaag tcctactttt tggtgacttg gccattcgtt
      601 tcgaagatga gcttcgccag ttagttcatg tcagggggaa tgagagtctc cgttcatttt
      661 tcgatcaagt ttgccatgca cttcgacagg agtttgggga tcttcctggc agccagcagg
      721 accttttccc tcgatttact acgcttgtcg atcttgtctc caagctcgca gagacagagg
      781 gcacccccgc tttgagattt acccttcttc cagtttgtga aatcggccaa tttattagat
      841 actatgggga agggtcaagg ccgtatccta gtgccatcga cagctactgc ttggggctct
      901 gtgcaggagg gtttgccgcc gcagcagtca gtacatccca gactttggcc gagttgatcc
      961 cagcaggagt cgaagcagtt ctggcggcgt ttcgaacggc tctctgctct ttagaagttc
     1021 gtaataatat agaaggaccg agccttgaga gacatcgatc ttggtctgct gtggtcaatg
     1081 ctccggaaaa agaagcttct gaattaatcc agcggtttat tttggataag ggtctaccac
     1141 acagctgtag gccctatttg agcgcaataa caccgtcgaa tgtaactatc agcggccctc
     1201 ccggcgtctt aaaagagttc tttgcagaaa gttcactaaa gttttaccac atacctgtcg
     1261 aattaccata ccatgcccct catcttttcg agccacgcga cgtcgacgaa atcatctgcc
     1321 gattcgatga caaattattt ggttcgtaca aaccacgaat tcctatttgg tccgcagcat
     1381 ctggaaaccc cattgcagcc gaaaatttta agactctgtt gcataaagtt gtgacggaca
     1441 cattacgtga accagtacag tggacgagta tcctcgaatc aagcgccaaa cacctcggac
     1501 ataggaattt tcaagaatgc acaataaacg ctctatcatc caatgcctcc tctctcctct
     1561 cttcggccat atctcggagc accaaaattc ttgtcgccgt taatgacttg gcttcgaaat
     1621 ctggatcagt accacagcct cggagacaca gtggccgttt agaccaatcc aagattgcta
     1681 tagtcggtta ttccggtcgt tttccagact ctgcgtcaaa tgaagaattt tgggaattgc
     1741 tgcgcgccgg gaaggatgtt catcgccttg tccctgagga tcgttttgac tgggaagcac
     1801 actttgaccc cactggaaag gccaaaaaca ccagccgggt taaatatggg tgcttcatca
     1861 aggaaccagg gctatttgat gctcgctttt ttaacatatc cccaagagaa gccgaaaaca
     1921 cagacccagc tcaaagattg gccattacaa ctacctatga ggcgcttgag atggcaggaa
     1981 tggtgcccaa tagaacgcca tcgacacaac aggaccgcat cggtgtcttc tatggtgtta
     2041 ctagcgacga ctggagagaa gttaatagtg gccaaaatgt cgatacctat tttattcctg
     2101 gtggcaaccg tgcatttgtt ccaggtagga ttagttactt ttttagattc tcgggcccca
     2161 gtctcagcat agacaccgcc tgttcctcca gctttgctgc tattcaaatt gcttgcagta
     2221 atctgtggag aggcgagtgt gacactgcca ttgcaggcgg aactaacgtc ctgacgaacc
     2281 ccgacaattt tgccggccta gatcgaggcc attttctgac aactacaggc aattgcaatg
     2341 cttttgacga tgaagctagc gggtattgtc gcgctgacgc tgtgggatcc gtcattctga
     2401 agagactgga ggatgcccaa gctgataacg accctatttt cggtgttatt gccggggcat
     2461 acacgaatca ttgtggccaa acggattcca taactagacc tcatgaaggc gaccaaatat
     2521 cagttttcaa gcgaatcatt cgacacgcaa acgtgaaccc gctagatatt agctacgtcg
     2581 aaatgcacgg aacaggaaca caagcgggag atgcgacgga aatgaattcc gttttatcag
     2641 tcttcgtccc tgaacgtgaa cgaatgccag attatccgct ctacctaggc tcagccaaag
     2701 ccaatatcgg ccatgcagaa tcagcatccg gagtctcttc ccttatcaag gtcttaatga
     2761 tgatgaaaca caatgaaata cccccccatt gcggaataaa aacacggatt aaccacaatt
     2821 atccactcga ccttacagag cgaaatgtta atattgctgc cacacccatc ccttggcatc
     2881 gcacagcgtc agggaagcgc tctgttttct tgaacaattt cagcgccgca gggggcaaca
     2941 cagccgtact cttggaagat gccccttttg caccacattc aaacgatatt agaagtgact
     3001 ctcgcagcag ccatctcgtt gctgttactg gcaagtcggc aaagtcacta aacaacaaca
     3061 tcaaggttct tattgacttt cttcagctga atcccgaaac tccgctgcct gcactgtctt
     3121 acacgacgac tgcgaggcgg atgcactatt cgtacaggac aatatgttca ggatcagaca
     3181 tcaaatttat catagatgca ttgaaaaaag ctgcagattc ggactcgaag ccatctccaa
     3241 gcaccaaagt tccaaaaata gcattcgctt tctctggcca aggcagcctc tattcaggca
     3301 ttggaaggca attattcgag tctcacccta gctttaaagc ggatatactt cgctttgata
     3361 gcatagcgca gcaacaagga ttcccaggct ttttgccttt agttgacggt actgcttcgg
     3421 atgtagatgg tgttgaaact gtcgcagctc accttgccct cagctgcgtg caaatggcga
     3481 tctgtcgcct ttggatttct tggggagttc gtccaaattt aacaattgga catagtcttg
     3541 gagaatatgc cgcactacaa gttgctggtg ttctcacacc cagcgacgtc atatatcttg
     3601 tcgggaccag ggcccagttg ctggcaagat attgcaacca aggaacccac gccatgctgt
     3661 ccataaaggc atcgtcggat gatttaaaaa cctatctgtc aggatcaacc tgtgaaatag
     3721 cttgtatgaa ccagcctaca aataatgtaa taagcggacc gaacgaggag gtaaaccgtc
     3781 taatgctttt gtttcaatcg aagggttatg aatgcattaa gcttggaatc ccctttgcct
     3841 ttcattcagc ccaagtggat cccattctag aggaatttga gtctgctgca agcagcatct
     3901 gttatcatgc cccgacgtta ccgtacatat ctcctttact cggattggtc gtctcaggcg
     3961 aaggagaact gaacgcatcg tatctgactc gggcatgtcg aagagcagta aactttcaag
     4021 gctccctaaa gtcagctgaa gcggcctctg ctattgacag tcatactatc tggattgaaa
     4081 ttggatcaca ccctgcatgc tctggtatgg tcaaaggaac aatcggtcca cagtcgacga
     4141 caattccatc attaagaaag ggcgttgata cgtggaaagt ccttactgca gggctcgaaa
     4201 cgctatacct cagtcgcgtt aacatagagt ggaatgagtg gcatcgacat tttaagagct
     4261 cacacaaagt tctccaactc ccaagataca gctgggactt gaagaattac tggatccagt
     4321 accgaaataa cttctgtctt accaaaggag aaggcacatc agccaatgct gcgcttccgg
     4381 tcgacacgaa gcccagctat gcttccctgt cgccagctgt ccaacgtgtt ctggaagagc
     4441 accatgctgc agacgtttcc aacatcctcg tagagtcaga cattcaagat ccgcacctgg
     4501 cgcctgtgct tcaaggccat aaggttaacg gcgcttccct ctgcccctcg tctctttacg
     4561 cggatattgc tctgacactt gccaactaca tgatgaaggc cgtcaatatg caaggtagcg
     4621 agataggcct tgatgttgct gacatgaaag tcgacaagcc tctcatagca cttgcggccg
     4681 gtgatccaca gctgtttcga gtttctgcgt cagctaattg ggcatcaaaa actatttcac
     4741 tagcattcta cagcatcaat ggccagggtg ctaaaaaggc ggaccatgct aaatgccttg
     4801 tcaaactcgc ctcgaaccaa acttggcttc aggattggaa gcgcaatgca tatctgatca
     4861 agagcaggat tgctgctcta cacaaaggtg tcgatgaagg cgaatcacac aaactgaagc
     4921 gcggacttgt ctacaagcta tttggggcaa tagtagacta tgatccgcaa taccaaggca
     4981 ttcaggaagt aattttagac agtaatgagc ttgaagctac ggcaaaggtt actttccagg
     5041 tcggcgacgg aggctattgt ttcagtcctc attgggtaga cagccttgga catattgctg
     5101 ggtttatcat gaatggcaac gacaatgttg agtccaaaag ccaagttttc atcaatcatg
     5161 gttggagcgc aatgcgctgt gcaacgactt tcgcatatgg caaaacttac cagacgtaca
     5221 ataagatgca acttgtagaa ggcacattgt atgcagggga cacatatatt cttgaagaag
     5281 gaaatatcgt tgctattttc gaaggcgtca agtttcaagg gattccaagg agagttctag
     5341 accaggtttt gccctcgaag cccagcacag tcaagaatgc agcgccgcct caaaggccgg
     5401 tggcgaccac tacctctgtc tcagcggcaa ggcaatcgcc aaagtttcca acagcacctg
     5461 atgtaactct tgtcaggccg ccatcaaatg atatcatctc tcaagtcatg gcaatagtct
     5521 cggatgaggc ccacattgac ccatcagagc tcaagcccga gactgagttt gcagattttg
     5581 gcatcgactc ccttctctct ctgacaatat tgggcagatt acgggacgag gctggacttg
     5641 acctgccatc tatgcttttt tcagactatc caactgtcga gaagctccga aatttcattg
     5701 gtgtggacca aagcccgtct ctgtcatccg ggtcgtcgag tcaagatgat acagaggtgt
     5761 tgacatcaga agtagcgtct gagccagata cctataggct tgctgatatc atccgcaata
     5821 ctatctcaga ggagactggg gtccccattg aagagctcac cgtctcagcc aggctctcgg
     5881 aacttggtat agactctctt cttgcattga caaccatggg caagctcagt gaagcccttg
     5941 atatcgaggt tccacagttg cttttcgtcg agaacgacac attgcatgat cttgaaaggg
     6001 ccttggatat gacagtaaag cacacacatg tcaaaatgtg ccccaatgtc gccagacttc
     6061 aggccattga gcaagtcaac gtcgactcac ctccatacgc aacctcggtc ttacttcagg
     6121 gaaattcgaa aaccgcaaca aaaaagatct ttctgttccc cgacggttcc gggtctgcta
     6181 cttcatacag ctcccttgct cgcgtctctc ctgatgtcgc tgtatacgga ctaaactgcc
     6241 cgtggatgaa gacaccagaa gatatgaagg tcacgctcga acaattgact gccaagtacc
     6301 tccttgaaat ccgacgccga cagcctcatg gcccctatta ctttggtggc tggtctgccg
     6361 gcggcatatg cgcctacgaa gcagcacagc agttgtctcg cgatggcgag gagactgcgc
     6421 gcctcatact catcgactcg ccgaacccag taggcttgga aaaccctcct cagcgcatgt
     6481 acgacttctt tgatactctc gggatctttg gggaaggtgg caagccgccg ccaagctggc
     6541 ttcggccgca cttcgacgcg tttattcgac tgctggacga ctacaaggtt gaagcttttg
     6601 cgagcaagtc gatggaaacg catatggtgt acgccagaga tggcatctgc aaactgccta
     6661 ctgaccccag gcctgagatt cgtcctgatg acccaagaga gatgctgtgg ttattgaata
     6721 accggactga cttcagtggt agtggatggt gtgagctcgt aggaaaggac aatatgaaga
     6781 tcacggtttt ggataatgtg aatcacttca ccatggtgga accgggacca gagatttcca
     6841 agcttagcaa ttttatcaga aaggctatgg agtgaggaga ttgataagac ttttctagtt
     6901 gcatatcttt tatatttaaa tcttatctat tagttaattt tttgtaattt atccttatat
     6961 atagtctggt tattctaaaa tatcatttca gtatctaaaa attcccctct tttttcagtt
     7021 atatcttaac aggcgacagt ccaaatgttg atttatccca gtccgattca tcagggttgt
     7081 gaagcatttt gtcaatggtc gaaatcacat cagtaatagt gcctcttact tgcctcatag
     7141 aatttctttc tcttaacgtc accgtttggt cttttatagt ttcgaaatct atggtgatac
     7201 caaatggtgt tcccaattca tcgttacggg cgtatttttt accaattgaa gtattggaat
     7261 cgtcaatttt aaagtatatc tctcttttac gtaaagcctg cgagatcctc ttaagtatag
     7321 cggggaagcc atcgttattc gatattgtcg taacaaatac tttgatcggc gctatgcggc
     7381 cgccaccgcg gtggagctcc agcttttgtt ccctttagtg agggttaatt gcgcgcttgg
     7441 cgagactcaa acaagatata ttttgcccgc cttacagacg aaactagttg gaggttatgg
     7501 agcatactat cacgtgggcg gccactggtg agttactaca ccccaggggc aacgttgatg
     7561 ctcctaaaaa actctggctg gacgcaagcc gtaacacccg tgtcacttca taatcgtttg
     7621 caattcaggg cttgatctac acattaccct gttatcccta ttaattaata cggttatcca
     7681 cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga
     7741 accgtaaaaa ggccgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc
     7801 acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg
     7861 cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat
     7921 acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcatagctca cgctgtaggt
     7981 atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc
     8041 agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg
     8101 acttatcgcc actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg
     8161 gtgctacaga gttcttgaag tggtggccta actacggcta cactagaagg acagtatttg
     8221 gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg
     8281 gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca
     8341 gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga
     8401 acgaaaactc acgttaaggg attttggtca tgagattatc aaaaaggatc ttcacctaga
     8461 tccttttaaa ttaaaaatga agttttaaat caatctaaag tatatatgag taaacttggt
     8521 ctgacagtta ccaatgctta atcagtgagg cacctatctc agcgatctgt ctatttcgtt
     8581 catccatagt tgcctgactc cccgtcgtgt agataactac gatacgggag ggcttaccat
     8641 ctggccccag tgctgcaatg ataccgcgag acccacgctc accggctcca gatttatcag
     8701 caataaacca gccagccgga agggccgagc gcagaagtgg tcctgcaact ttatccgcct
     8761 ccatccagtc tattaattgt tgccgggaag ctagagtaag tagttcgcca gttaatagtt
     8821 tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg
     8881 cttcattcag ctccggttcc caacgatcaa ggcgagttac atgatccccc atgttgtgca
     8941 aaaaagcggt tagctccttc ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt
     9001 tatcactcat ggttatggca gcactgcata attctcttac tgtcatgcca tccgtaagat
     9061 gcttttctgt gactggtgag tactcaacca agtcattctg agaatagtgt atgcggcgac
     9121 cgagttgctc ttgcccggcg tcaatacggg ataataccgc gccacatagc agaactttaa
     9181 aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt
     9241 tgagatccag ttcgatgtaa cccactcgtg cacccaactg atcttcagca tcttttactt
     9301 tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa
     9361 gggcgacacg gaaatgttga atactcatac tcttcctttt tcaatattat tgaagcattt
     9421 atcagggtta ttgtctcatg agcggataca tatttgaatg tatttagaaa aataaacaaa
     9481 taggggttcc gcgcacattt ccccgaaaag tgccacctga acgaagcatc tgtgcttcat
     9541 tttgtagaac aaaaatgcaa cgcgagagcg ctaatttttc aaacaaagaa tctgagctgc
     9601 atttttacag aacagaaatg caacgcgaaa gcgctatttt accaacgaag aatctgtgct
     9661 tcatttttgt aaaacaaaaa tgcaacgcga gagcgctaat ttttcaaaca aagaatctga
     9721 gctgcatttt tacagaacag aaatgcaacg cgagagcgct attttaccaa caaagaatct
     9781 atacttcttt tttgttctac aaaaatgcat cccgagagcg ctatttttct aacaaagcat
     9841 cttagattac tttttttctc ctttgtgcgc tctataatgc agtctcttga taactttttg
     9901 cactgtaggt ccgttaaggt tagaagaagg ctactttggt gtctattttc tcttccataa
     9961 aaaaagcctg actccacttc ccgcgtttac tgattactag cgaagctgcg ggtgcatttt
    10021 ttcaagataa aggcatcccc gattatattc tataccgatg tggattgcgc atactttgtg
    10081 aacagaaagt gatagcgttg atgattcttc attggtcaga aaattatgaa cggtttcttc
    10141 tattttgtct ctatatacta cgtataggaa atgtttacat tttcgtattg ttttcgattc
    10201 actctatgaa tagttcttac tacaattttt ttgtctaaag agtaatacta gagataaaca
    10261 taaaaaatgt agaggtcgag tttagatgca agttcaagga gcgaaaggtg gatgggtagg
    10321 ttatataggg atatagcaca gagatatata gcaaagagat acttttgagc aatgtttgtg
    10381 gaagcggtat tcgcaatatt ttagtagctc gttacagtcc ggtgcgtttt tggttttttg
    10441 aaagtgcgtc ttcagagcgc ttttggtttt caaaagcgct ctgaagttcc tatactttct
    10501 agagaatagg aacttcggaa taggaacttc aaagcgtttc cgaaaacgag cgcttccgaa
    10561 aatgcaacgc gagctgcgca catacagctc actgttcacg tcgcacctat atctgcgtgt
    10621 tgcctgtata tatatataca tgagaagaac ggcatagtgc gtgtttatgc ttaaatgcgt
    10681 acttatatgc gtctatttat gtaggatgaa aggtagtcta gtacctcctg tgatattatc
    10741 ccattccatg cggggtatcg tatgcttcct tcagcactac cctttagctg ttctatatgc
    10801 tgccactcct caattggatt agtctcatcc ttcaatgcta tcatttcctt tgatattgga
    10861 tcatactaag aaaccattat tatcatgaca ttaacctata aaaataggcg tatcacgagg
    10921 ccctttcgtc tcgcgcgttt cggtgatgac ggtgaaaacc tcggcgcgcc attaccctgt
    10981 tatccctaat atacaacgca aagaagctgg aaaattggcg agggaatcct gtttctgtct
    11041 atccaagaat gggcatgagg tggcaaccgt cgtgctagcg tacagggtgc actttgtaac
    11101 catttgggac accggacact cgctgttttc gaaattaccc tttaagcgcg ggtattgaac
    11161 caggcttatg cccaagatcg tagcaagcat ttaactgtgg gaatactcag gtatcgtaag
    11221 atgcaagagt tcgaatctct tagcaaccat tatttttttc ctcaacataa cgagaacaca
    11281 caggggcgct atcgcacaga atcaaattcg atgactggaa attttttgtt aatttcagag
    11341 gtcgcctgac gcatatacct ttttcaactg aaaaattggg agaaaaagga aaggtgagag
    11401 gccggaaccg gcttttcata tagaatagag aagcgttcat gactaaatgc ttgcatcaca
    11461 atacttgaag ttgacaatat tatttaagga cctattgttt tttccaatag gtggttagca
    11521 atcgtcttac tttctaactt ttcttacctt ttacatttca gcaatatata tatatatttc
    11581 aaggatatac cattctaatg tctgccccta tgtctgcccc taagaagatc gtcgttttgc
    11641 caggtgacca cgttggtcaa gaaatcacag ccgaagccat taaggttctt aaagctattt
    11701 ctgatgttcg ttccaatgtc aagttcgatt tcgaaaatca tttaattggt ggtgctgcta
    11761 tcgatgctac aggtgtccca cttccagatg aggcgctgga agcctccaag aaggttgatg
    11821 ccgttttgtt aggtgctgtg gctggtccta aatggggtac cggtagtgtt agacctgaac
    11881 aaggtttact aaaaatccgt aaagaacttc aattgtacgc caacttaaga ccatgtaact
    11941 ttgcatccga ctctctttta gacttatctc caatcaagcc acaatttgct aaaggtactg
    12001 acttcgttgt tgtcagagaa ttagtgggag gtatttactt tggtaagaga aaggaagacg
    12061 atggtgatgg tgtcgcttgg gatagtgaac aatacaccgt tccagaagtg caaagaatca
    12121 caagaatggc cgctttcatg gccctacaac atgagccacc attgcctatt tggtccttgg
    12181 ataaagctaa tcttttggcc tcttcaagat tatggagaaa aactgtggag gaaaccatca
    12241 agaacgaatt ccctacattg aaggttcaac atcaattgat tgattctgcc gccatgatcc
    12301 tagttaagaa cccaacccac ctaaatggta ttataatcac cagcaacatg tttggtgata
    12361 tcatctccga tgaagcctcc gttatcccag gttccttggg tttgttgcca tctgcgtcct
    12421 tggcctcttt gccagacaag aacaccgcat ttggtttgta cgaaccatgc cacggttctg
    12481 ctccagattt gccaaagaat aaggttgacc ctatcgccac tatcttgtct gctgcaatga
    12541 tgttgaaatt gtcattgaac ttgcctgaag aaggtaaggc cattgaagat gcagttaaaa
    12601 aggttttgga tgcaggtatc agaactggtg atttaggtgg ttccaacagt accaccgaag
    12661 tcggtgatgc tgtcgccgaa gaagttaaga aaatccttgc ttaaaaagat tctctttttt
    12721 tatgatattt gtacataaac tttataaatg aaattcataa tagaaacgac acgaaattac
    12781 aaaatggaat atgttcatag ggtagacgaa actatatacg caatctacat acatttatca
    12841 agaaggagaa aaaggaggat agtaaaggaa tacaggtaag caaattgata ctaatggctc
    12901 aacgaaatga cggtatcgat aagcttgata tcgaattcct gcagcccggg ggatccacta
    12961 gttctagagt taattaa
//
