LOCUS       sequence-208193-       21204 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of a fungal biosynthetic gene cluster.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 21204)
  AUTHORS   Harvey CJB, Tang M, Schlecht U, Horecka J, Fischer CR, Lin HC, Li J,
            Naughton B, Cherry J, Miranda M, Li YF, Chu AM, Hennessy JR, Vandova
            GA, Inglis D, Aiyar RS, Steinmetz LM, Davis RW, Medema MH, Sattely 
            E, Khosla C, St Onge RP, Tang Y, Hillenmeyer ME
  TITLE     HEx: A heterologous expression platform for the discovery of fungal 
            natural products.
  JOURNAL   Sci Adv. 2018 Apr 11;4(4):eaar5459. doi: 10.1126/sciadv.aar5459. 
            eCollection 2018 Apr.
  PUBMED    29651464
REFERENCE   2  (bases 1 to 21204)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Adv."; 
            date: "2018-04-11"; volume: "4(4):eaar5459. doi"; pages: " 
            10.1126/sciadv.aar5459. eCollection 2018 Apr"
COMMENT     Sequence Label: pCHKU38.2-2.2
FEATURES             Location/Qualifiers
     source          1..21204
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      1860..2159
                     /label=ADH2 terminator
                     /note="transcription terminator for the S. cerevisiae 
                     alcohol dehydrogenase 2 (ADH2) gene"
     promoter        4891..5111
                     /gene="S. cerevisiae URA3"
                     /label=URA3 promoter
     CDS             5112..5915
                     /codon_start=1
                     /gene="S. cerevisiae URA3"
                     /product="orotidine-5'-phosphate decarboxylase, required 
                     for uracil biosynthesis"
                     /label=URA3
                     /note="yeast auxotrophic marker, counterselectable with 
                     5-fluoroorotic acid (5-FOA)"
                     /translation="MSKATYKERAATHPSPVAAKLFNIMHEKQTNLCASLDVRTTKELL
                     ELVEALGPKICLLKTHVDILTDFSMEGTVKPLKALSAKYNFLLFEDRKFADIGNTVKLQ
                     YSAGVYRIAEWADITNAHGVVGPGIVSGLKQAAEEVTKEPRGLLMLAELSCKGSLSTGE
                     YTKGTVDIAKSDKDFVIGFIAQRDMGGRDEGYDWLIMTPGVGLDDKGDALGQQYRTVDD
                     VVSTGSDIIIVGRGLFAKGRDAKVEGERYRKAGWEAYLRRCGQQN"
     terminator      12420..12607
                     /gene="S. cerevisiae ADH1"
                     /label=ADH1 terminator
                     /note="transcription terminator for the S. cerevisiae 
                     alcohol dehydrogenase 1 (ADH1) gene"
     primer_bind     complement(12475..12499)
                     /label=pHybLex-R
                     /note="ADH terminator, reverse primer"
     terminator      15357..15606
                     /gene="S. cerevisiae CYC1"
                     /label=CYC1 terminator
                     /note="transcription terminator for CYC1"
     primer_bind     complement(15373..15391)
                     /label=CYC1
                     /note="CYC1 transcription termination signal, reverse 
                     primer"
     primer_bind     complement(17351..17371)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(17351..17369)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     rep_origin      complement(17708..18296)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(17788..17807)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(18467..19258,19259..19327))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     19090..19109
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(19328..19432)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      19459..20801
                     /label=2u ori
                     /note="yeast 2u plasmid origin of replication"
     protein_bind    complement(20422..20469)
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     primer_bind     20842..20860
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(21179..21195)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(21179..21195)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
ORIGIN
        1 tatctaaaaa ttgccttatg atccgtctct ccggttacag cctgtgtaac tgattaatcc
       61 tgcctttcta atcaccattc taatgtttta attaagggat tttgtcttca ttaacggctt
      121 tcgctcataa aaatgttatg acgttttgcc cgcaggcggg aaaccatcca cttcacgaga
      181 ctgatctcct ctgccggaac accgggcatc tccaacttat aagttggaga aataagagaa
      241 tttcagattg agagaatgaa aaaaaaaaaa aaaaaaaagg cagaggagag catagaaatg
      301 gggttcactt tttggtaaag ctatagcatg cctatcacat ataaatagag tgccagtagc
      361 gacttttttc acactcgaaa tactcttact actgctctct tgttgttttt atcacttctt
      421 gtttcttctt ggtaaataga atatcaagct acaaaaagca tacaatcaac tatcaactat
      481 taactatatc gtaatacaca atggaggatt cctttatcct gaagaacgtt tcaatattta
      541 caggtgatga ctggatagat tctggttgca tagcagttag gaatggcata atcaactacg
      601 tgggccgcgg ggatcctcca gctcgaatag tacccgaggg cactgcagtt ctctcattgc
      661 cgcggagaag tgtgattcct ggtctcatcg attcacgagt tcattgtcag gaaggtaaca
      721 ttgaatgtct cgagcagtcg ctgagattcg gcgtgacgac tgtctgcgac atgtatggcg
      781 accctcagta tcttaatctt ctaacacagc aagcacaaga ccctgtgaac aaagacaagt
      841 atgccgactt taaatgtgca ggcatcgcag ctagtatcga gagcgagtgg cctggaacgg
      901 agcttcaatt atcccctgag aacaaacctg taagcctaga gttcagagca gagagtttaa
      961 aagcagtcac tcaccaagaa atgaccgcat ggcagtctag tgtcccggag gatatcttgc
     1021 ccaaactgaa atctgctgag gatgcagagc attacgtccg agaacaagtc gtcaaattca
     1081 acgcatcata tatcaaaatc atgcacggga tcggcgacga tgtcgacgtg gaatttacgg
     1141 agccaagatg cgaagtgcaa aacgcagtgg tagatgcggc gcacaggcgt ggtttgatcg
     1201 ttttaggaca tgcgttgagc tattccgcgg cgatggatct gctctcaaac ggtgttgatg
     1261 ggttgaccaa catcttcatt gatagatcga catctcgcga ctggattgag cttctaaagc
     1321 gcaacaaggg acacttgtgc ccaactttgt cgttttatat ctttcgcact ctccaaggcg
     1381 acgatattga agagctgttc gcaagcgatc cgttctcaga gcgtttgctc ttcgacaaaa
     1441 gttctcagag gaacgtcagt ctcggttcca agcctgaaga tgcagctttc gccaacgctg
     1501 tgcgcaatgt acaagaggca tacaaagccg gggtgccatt tgtcatcgga tcggccagca
     1561 caggcagaga gattggcccg gcatatgggt tgggtgtcca tatcgagatg tatctgcttt
     1621 caaaagcagt tggaatgact ccaatggagg tcttgaagag tgcaacaagt gaagtcgcgg
     1681 atcgttttgg attccatgat cgtggccgtg ttgcagtagg caagaaggcg gatctggttc
     1741 tggttgaggg tgacgtccga gaagccattg cagatccaaa ctgccggtgt ctcccagtct
     1801 catgcgtgtg gagagaaggt gttagctcga taatttacca aagactggct gggccatagg
     1861 cggatctctt atgtctttac gatttatagt tttcattatc aagtatgcct atattagtat
     1921 atagcatctt tagatgacag tgttcgaagt ttcacgaata aaagataata ttctactttt
     1981 tgctcccacc gcgtttgcta gcacgagtga acaccatccc tcgcctgtga gttgtaccca
     2041 ttcctctaaa ctgtagacat ggtagcttca gcagtgttcg ttatgtacgg catcctccaa
     2101 caaacagtcg gttatagttt gtcctgctcc tctgaatcgt ctccctcgat atttctcatt
     2161 ttccttcgca tgccagcatt gaaatgatcg aagttcaatg atgaaacggt aattcttctg
     2221 tcatttactc atctcatctc atcaagttat ataattctat acggatgtaa tttttcactt
     2281 ttcgtcttga cgtccaccct ataatttcaa ttattgaacc ctcacgatcc agttctccag
     2341 tgacacagcc tttatctggt caaacctttc tttctaatca cctatgctga tgcttaatta
     2401 agggattttt gtctccatca acggcatgcg cccaaaaatg acgttttttt taacccatag
     2461 acacgaaact acccattttc caccggcctg acctaccacc ggaacaacgg ccatctccaa
     2521 cttgcaagtt ggggaaatta agagcatcgc aggtttaatg gaagaaaaaa aaaaggtaca
     2581 gcacagcgca aatggagtta gttcccttat gtcacacact cacacacagt cggtcagatc
     2641 aagcatactg ggtgcgtata aatagagtgg ccattgccac cctgtttatc tcaaaatctg
     2701 tcttgttagt ggtcttctcc ctttttcagg ttacaattct cttgtttcta cttagtatat
     2761 aagtatatca agctatatta agcatactat caactgtcaa ctctatcctc aaaatacaat
     2821 acaaaatgac tactattcca gagaaggcaa ctgttcttgt gattggaggc gggccgggag
     2881 gttcttacgc cgctacggca cttgcaagag agggcgttga tgtggttttg cttgaggcag
     2941 acaaatttcc tcgttaccac atcggcgaga gcatgttggc gtccatgcgc cattttcttc
     3001 gcttcatcga tcttgactcc acattcgaca atcatggttt catcaaaaag cgcggggcag
     3061 ccttcaagct gaatgacaaa cccgaaggct ttaccgattt cattgctgcg ggaggaccgg
     3121 gaaactatgc ctggaatgtg attcgctcag aagcggacta cctaatgttt cagcatgctg
     3181 gaaagagcgg cgtaaagaca ttcgatcaag tagccgtcag atcattggaa ttcactccat
     3241 gtgacctcga actaccggat ccgaaaacac caagccctgg cagagcggtc gcagcttgct
     3301 ggtctagcaa ggttgacggt acatctggca aaatcagttt cgattattta gttgatgcga
     3361 gtggtcgttt cggcataacg agcacgaagt atcttaaaaa ccgcaagttc aatcagggat
     3421 tgaagaacat cgccaactgg tcgtattgga agaacgcggg aagatacgcg gttggaaccc
     3481 agagagaggg tcagcccttt tttgaggccc taagtgatgc aagcggctgg tgctggttga
     3541 ttccactcca tgatggtact gcctctgtcg gtattgttca gaatcaggat gcggccgctg
     3601 ccaagaaaaa agccatggat tcgccatcac aacatgactt ctacattgaa agcttaaagc
     3661 tagcgccgag gattcaggca ctgcttgccc aaggagagat gacggctcct gttaaacaag
     3721 cctctgactg gtcgtatagt gcatcatgct acgcttcgcc taacgttcga attgttggtg
     3781 atgctgggtg ctttatcgat ccctatttct cctctggggt gcaccttgcg ctttccggtg
     3841 ccatgtcggc agcttgcaca attcgtgctg tgcagcgtga agaatgcgac gaaaaagctg
     3901 caatggactg gcactcgaag aaggtggccg aaggctatac acgcttcctg ctcgtcgtcc
     3961 tcagtgccat gagacagatc cgcaagtcag atcaaccaat cctgagcgac tgggacgagg
     4021 aaggtttcga ccgcgctttt gcattcttcc gtccaatcat tcagggaacc gccgacgtca
     4081 gtaacaagct gagccaagaa gagttggcca agacgattga cttttgtctc aatgctttcc
     4141 aacatcacga cgccgatgcg agagacgcta tgatcaaaaa gctgcaggag cacgagcagg
     4201 aagctagaat gcgtggagat gttcaagcca aggcagatat tgatccagat tcactcactc
     4261 cagaagaatt gcaggttttg aagactattc gcgctcgaca aatgctgaga accgaagatg
     4321 ttatcaatat cgattccttc ggaactgacg caattgatgg gttcgctcca cgggtgaagc
     4381 acggagagct gggacttatg aagggtaaga actccggccc agctgctccc aagatggatc
     4441 tctttgcaaa ggcgaaagag gagcctgcaa tgcagacgca agtgggtact gcagtcacag
     4501 cgcattgaac aaatcgctct taaatatata cctaaagaac attaaagcta tattataagc
     4561 aaagatacgt aaattttgct tatattatta tacacatatc atatttctat atttttaaga
     4621 tttggttata taatgtacgt aatgcaaagg aaataaattt tatacattat tgaacagcgt
     4681 ccaagtaact acattatgtg cactaatagt ttagcgtcgt gaagacttta ttgtgtcgcg
     4741 aaaagtaaaa attttaaaaa ttagagcacc ttgaacttgc gaaaaaggtt ctcatcaact
     4801 gtttaaaagg aggatatcag gtcctatttc tgacaaacaa tatacaaatt tagtttcaaa
     4861 gatgaatcag tgcgcgaagg acataactca ttcaattcaa ttcatcattt tttttttatt
     4921 cttttttttg atttcggttt ctttgaaatt tttttgattc ggtaatctcc gaacagaagg
     4981 aagaacgaag gaaggagcac agacttagat tggtatatat acgcatatgt agtgttgaag
     5041 aaacatgaaa ttgcccagta ttcttaaccc aactgcacag aacaaaaacc tgcaggaaac
     5101 gaagataaat catgtcgaaa gctacatata aggaacgtgc tgctactcat cctagtcctg
     5161 ttgctgccaa gctatttaat atcatgcacg aaaagcaaac aaacttgtgt gcttcattgg
     5221 atgttcgtac caccaaggaa ttactggagt tagttgaagc attaggtccc aaaatttgtt
     5281 tactaaaaac acatgtggat atcttgactg atttttccat ggagggcaca gttaagccgc
     5341 taaaggcatt atccgccaag tacaattttt tactcttcga agacagaaaa tttgctgaca
     5401 ttggtaatac agtcaaattg cagtactctg cgggtgtata cagaatagca gaatgggcag
     5461 acattacgaa tgcacacggt gtggtgggcc caggtattgt tagcggtttg aagcaggcgg
     5521 cagaagaagt aacaaaggaa cctagaggcc ttttgatgtt agcagaattg tcatgcaagg
     5581 gctccctatc tactggagaa tatactaagg gtactgttga cattgcgaag agcgacaaag
     5641 attttgttat cggctttatt gctcaaagag acatgggtgg aagagatgaa ggttacgatt
     5701 ggttgattat gacacccggt gtgggtttag atgacaaggg agacgcattg ggtcaacagt
     5761 atagaaccgt ggatgatgtg gtctctacag gatctgacat tattattgtt ggaagaggac
     5821 tatttgcaaa gggaagggat gctaaggtag agggtgaacg ttacagaaaa gcaggctggg
     5881 aagcatattt gagaagatgc ggccagcaaa actaaaaaac tgtattataa gtaaatgcat
     5941 gtatactaaa ctcacaaatt agagcttcaa tttaattata tcagttatta ccctatgcgg
     6001 tgtgaaatac cgcacagatg cgtaaggaga aaataccgca tcaggatagg aaaaaaccga
     6061 gcttcctttc atccggcgcg gctgtgttct acatatcact gaagctccgg gtattttaag
     6121 ttatacaagg gaaagatgcc ggctagacta gcaagtttta ggctgcttaa cattatggat
     6181 aggcggataa agggcccaaa caggattgta aagcttagac gcttctggtt ggacaatggt
     6241 acgtttgtgt attaagtaag gcttggctgg ggatagcaac attgggcaga gtatagaaga
     6301 ccacaaaaaa aaggtatata agggcagaga agtctttgta atgtgtgtaa cttctcttcc
     6361 atgtgtaatc agtatttcta cttacttctt aaatatacag aagtaagaca gataaccaac
     6421 agcctttccc agatatacat atatatcttt atttcagctt aaacaataat tatatttgtt
     6481 taactcaaaa ataaaaaaaa aaaaccaaac tcacgcaact aattattcca taataaaata
     6541 acaacatggg caatcaaatc tcagcattga tgtctgatag cattgtcttc agtcttttct
     6601 tgcgaatatc atatctaggc ctaattctgt cgataatcta tgttttggca gctttgacct
     6661 acaacgttta ttttcaccca ctcaaaaact accccggacc attgtcgtgg gcagcgacgc
     6721 gtattccctg gtgctggtac caatatcacg gtgtcaattg gcgcaaaatt cttgagcttc
     6781 acgcaagata tggacatgtt gtgcgcattg gtccgaacga gcttagttac acctcctctg
     6841 aggcttggag gattatctat gggcatcgtg ttatcgaaat gggaaaagat ccggtgttct
     6901 cgctgcacac tccaactggc gttcagaaca tcctagtagc tgaccgtccg acgcatacaa
     6961 gacagcgacg actacttgct catgcgttct cagaaaaggc gttgcgtgag caggaagaga
     7021 tccttcaatc ctatactgat caattgatcc gtggcttaga agcccgccgg caagatgctg
     7081 tcgacattgg tgcatggttc atgtttacca cgttcgatct gatagccgat ctgtcatttg
     7141 gcgacaatgt gggctgcctc gaaaagggcg agtatgatcc atacgtggca gcaacaccag
     7201 caatttccaa ggagttgcag tactaccaga tgctaaagta ctggggcatc gcctgggctc
     7261 gaaaatggtt tatgccaacc tctgttgctg gagcccggat ggccaatatg aagcgcgcca
     7321 tgagtacggt caacaatcgc atcgaaaaaa atggttcagt ggatcgaaag gattttttgc
     7381 actacattct tgcagcgaat gatgaaaagg ggatgtcacg acaagaaatt gacgttaatg
     7441 cgttctcgat cagcactgcc ggttccgaat caacggccac agccctttca ggtgcaatat
     7501 tttacctctt gcaacacccg gcttcttatg cgagcgccac gcgtgaagtc cgctcagcgt
     7561 tccatgcacc tgaggagatc accatgagcg aagtcaagaa gttgaagtac ttgaaagcag
     7621 ttttacaaga aactctgcgc ctgtacccac ccgttgctgt tactttgccc cgtcgcgttc
     7681 cagttgaaga ggacggagta ttcattgcag gtcatcctgt tccagccgga acaacagttg
     7741 gtgtcaacta ttatgctgca ttccattcac cttacaactt tcaccttgcc gagaagtttg
     7801 ctcctgaacg atggcttcca gagcactcag atcatgcgcc ctacgcaaat gacaatagag
     7861 atgtagtcca gccattcagt tacggaccgc gcaattgttt agggaagaat ctcgcgtggg
     7921 ccgagatgca gctaattctt gcaagggtgt tatggcatta tgatattaca tggcccaaag
     7981 acaagcttgg aaggccagaa gttgatgaag gctggcagtt aaaccaaaaa ctgtacggct
     8041 tctgggtcaa accaccacta agggtttgct tgaagccggc cgatggaatc aggaaatgaa
     8101 gtgcttttaa ctaagaatta ttagtctttt ctgcttattt tttcatcata gtttagaaca
     8161 ctttatatta acgaatagtt tatgaatcta tttaggttta aaaattgata cagttttata
     8221 agttactttt tcaaagactc gtgctgtcta ttgcataatg cactggaagg ggaaaaaaaa
     8281 ggtgcacacg cgtggctttt tcttgaattt gcagtttgaa aaataactac atggatgata
     8341 agaaaacatg gagtacagtc actttgagaa ccttcaatca gctggtaacg tcttcgttaa
     8401 ttggatactc aaaaaagatg gatagcatga atcacaagat ggaaggaaat gcgggccacg
     8461 accacagtga tatgcatatg ggagatggag atgatacctc cattgggccg atgaagttag
     8521 tcgacggata gaagcggttg tcccctttcc cggcgagccg gcagtcgggc cgaggttcgg
     8581 ataaattttg tattgtgttt tgattctgtc atgagtatta cttatgttct ctttaggtaa
     8641 ccccaggtta atcaatcaca gtttcatacc ggctagtatt caaattatga cttttcttct
     8701 gcagtgtcag ccttacgacg attatctatg agctttgaat atagtttgcc gtgattcgta
     8761 tctttaattg gataataaaa tgcgaaggat cgatgaccct tattattatt tttctacact
     8821 ggctaccgat ttaactcatc ttcttgaaag tatataagta acagtaaaat ataccgtact
     8881 tctgctaatg ttatttgtcc cttatttttc ttttcttgtc ttatgctata gtacctaaga
     8941 ataacgacta ttgttttgaa ctaaacaaag tagtaaaagc acataaaaga attaagaaaa
     9001 tggcacattc tcaggatttt gaagttctta tcattggagc cggcatcacc ggcctcgtgc
     9061 ttgcacaagc tctgaagaag gttggaataa agtacaaaat cttcgaacga gaggtacaga
     9121 tgaactatcg gagcaacgag tggacgatgg caatccattg gtcgcttgag cgcctggaaa
     9181 atctccttcc aaaggagatc tgggagaaac tgcccacgat atcctgcaat ccagctattc
     9241 ctatggatgc tggaggcaag tatcccatca ttcatggtga aaccggcaag ctcctggccg
     9301 gtgtgccata tgccaggggc ttgcgtgtcc cacgcagcaa aatgagagct ctgtgtgctg
     9361 aaggaatcga tgtgcagtac ggcatgaagc ttactggagt cgatttcgat gcctctggag
     9421 ttggcgtaac ggctaccttt aatgactatg agaaagtcaa agcgtccctc atcattggca
     9481 ctgacggccc aaggtctacg atgcgcattt ttgccatggg aagcgaggca gaggcgtcta
     9541 ctagcaaatt tccaatcttt catacgaaca tgacagtttg ctacggcgac gctgaaaagg
     9601 caaagtattt gcgccaggaa tttcccacga gttatctcgc tctcagcgaa aatagctacc
     9661 acgctttcca gtcaatttcc agtatgcctg atggacctga gcatcctgaa acctgggtgt
     9721 tccatctggc catggcttgg ctcggggact ctgatcagtc catgagctat cctgaaagac
     9781 tcgctttgat caaaagcaag gctcagaccc ttggtgagcc tgcaagatct gcctttacat
     9841 ggataccaga gaacacgctt gttcacaagg cggacatcag ttactggatt agcaaaccgt
     9901 ggaataatca cgacggtaga atgaccttgg ctggcgatgc agcccacgcc atgccacctt
     9961 accgcgggca aggtctaaac cattgcatct gcgatgtgtc tcaccttctt gaaaagatcc
    10021 aaagcatctt gaataatgag atgggtcaaa aggaagccat ctctgcctat gatgacgagg
    10081 tcgttaaaag gggtgctgag gaggtaaaat gctcgctgga gaatgctctc atgctgcacg
    10141 actgggagaa agtgcagaga tcacctgtgt ttacaaccgg cttcaagcca atgactgggc
    10201 atgacgggga gaactctcat caaagaatgc ttcccgaagc cttgaagact cttcaagaag
    10261 tcaagcaaca gattgctagt cattagattt aactccttaa gttactttaa tgatttagtt
    10321 tttattatta ataattcatg ctcatgacat ctcatataca cgtttataaa acttaaatag
    10381 attgaaaatg tattaaagat tcctcaggga ttcgattttt ttggaagttt ttgttttttt
    10441 ttccttgaga tgctgtagta tttgggaaca attatacaat cgaaagatat atgcttacat
    10501 tcgaccgttt tagccgtgat cattatccta tagtaacata acctgaagca taactgacac
    10561 tactatcatc aatacttgtc acatgagaac tctgtgaata attaggccac tgaaatttga
    10621 tgcctgaagg accggcatca cggattttcg ataaagcact tagtatcaca ctaattggct
    10681 tttcgctagt cttatctaaa aattgccttt atagtccgtc tctccagtca cggcctgtgt
    10741 aactgattaa tcctgccttt ctaatcacca ttctactgtt taattaaggg attttgtctt
    10801 catcaacggc ttccgcccaa aaaaaagtat gacgttttgc ccgcaggcgt gaagctgccc
    10861 atcttcacgg gcctgacctc ctctgccgga acaccggcca tctccaactc ataaattgga
    10921 gaaataagag aatttcagat tttcagagga tgaaaaaaaa aaggtagaga gcataaaaat
    10981 ggggttcact ttttggcaaa gttacagtat gcttattaca tataaataga gtgccgataa
    11041 tggctttttt tcatcttcga aatacgcttg ctactgctct tccagcgttt ttattacttc
    11101 tttcttgttt ctccttagta tataaaatat caagctacaa caagcataca atcaactgtc
    11161 aactgtcaat tatattataa tacactatgc ccgcctccaa attggcacaa gagtctactc
    11221 aaaagccaga attcaggccg accgtttatg gactgatttc tgagctttgc gtgttgctga
    11281 ataagctaga gagtgatcgt gcggaggtgc cctcccttgc agcgggcgcg cattccaagc
    11341 tgtggactgc ccaaggcgat agcttagcgg cgtctcgcga tcgagctttg gagcttttga
    11401 ggaacctatg ccagctattg caaggaccgc atgaatactt tcattccttg gtgtcttcaa
    11461 attgggatct tggagctctc tacacaatca tcgagcttgg aatattggag aaaatccctt
    11521 tgacctcttg cgcaaacgtg attgatcttt ctgaagagct tggcttagat gagcggaaat
    11581 tgcttacact cctccgactt gtcacatgct caaacattct ttacgaggcc gaagacgggt
    11641 tctttgggca tactgccctg tctcaagagc ttttgcaaga cgagcaattc cgagccttca
    11701 taggattcca gctgtttgag gccaggatcg gcagtgtgca tttggcagac tccctgaagg
    11761 ggagcaaaaa cacctttgag catgggtgtt cagctttcaa acacgcaatg ggtataccgc
    11821 tataccagtg gcatggtgag aacacggaga aaggtacgag gttccgtatg gctatgcagg
    11881 gggtctcaaa aagtctagac cctggtgacg atttgttccg tcagtggttt aagcagcatt
    11941 ctgaatctgg cacatgtcta accgtcgtcg acgttcttgg aggcaacaaa ccaagccagc
    12001 aaaagttcat tggcgagttt ccagagctca atttcgtttc cgttcacgac attggcgaaa
    12061 tagcctttgt tgacggacct attctggcat atgtcgtccg caatgtgttc tggaacaaga
    12121 gagacaacga ggtgatctcc gtgattcgga gcatccacca agctatggag aaaaacgcaa
    12181 aagccacgct gttgattaat gaaatgctgt cgccagctag aggcgaattc ccaggagatc
    12241 tcgagcacgc ctatcgtcga cgcgacgtta caacgatgac tatgcacaac gcgaagcaac
    12301 ggactgtcgc tgaatggcat gggctatttc gtgaagtaga tctaaagtgg aagatccgac
    12361 tggtcacggg taattcgtca catagctaca gagggctttg ggagatacaa atgaattagg
    12421 cgaatttctt atgatttatg atttttatta ttaaataagt tataaaaaaa ataagtgtat
    12481 acaaatttta aagtgactct taggttttaa aacgaaaatt cttattcttg agtaactctt
    12541 tcctgtaggt caggttgctt tctcaggtat agcatgaggt cgctcttatt gaccacacct
    12601 ctaccggcat gcctctcaaa tcttttagcg ccaaggactc caactaattg tatcttgaat
    12661 ttgcctttac gatccgtttg tccagtcacg gcatgtatat cttattaatc ctgcctttct
    12721 aatcacgtat tctaatgttc aattaaggga ttttatcttc atcaacggct cccacgcaaa
    12781 aaatgacgtt ttgcacacag acacgaaata caccttccac cggaacaacg gccatctcca
    12841 acttataagt tggggaaata agacaatttc agacttcaga gaatgaaaaa aaaaaaaggt
    12901 acatcacaga tggggttcag gtttgctaca attgcaggga gcctgtcaca tataaataga
    12961 cctccagtga tgatatcttt cagtcttcaa acgtctcttg tcacagttct ggtcgttcta
    13021 tatcacatct ctcttggttc tacttattgt ctataatatc aagctacagc aagcatacaa
    13081 tcaactatct accataccat aatacacaat gataattctg gggcttccga ccgcgcttgt
    13141 gcactttgtc gggagaaaaa aatccgttgt ggccgagaga aacccaaatg tggcaattgt
    13201 aaacgtcaga aatggcgctg tgactactct gcgccagaaa agcgtaagtg aaaatgtcaa
    13261 ctctatcgat gctcgattgg gcaacattga gaacgaaatc tttcgtctta taagatccca
    13321 aaacgcgccc aggtctcgac atggctcgga gggcacgcct acatcgtatg agagtccgtt
    13381 caatccaagt gacaccgaag atgagtcgcg gggcagcctg ttgacaacag aaagacaact
    13441 cttgcactcg ccagtcacgg attcatacta cggaccggat acgttttatg catgggccac
    13501 tgccttcaaa gggctagtga catctcggcg acaaaaagta ggaaaggatg tctctcaaca
    13561 agggctgaat tttgcggttg atgatgagct gggctccatc gaccatgatt tatcggaatt
    13621 agtacgatat gccgagagac aggacacgct gtacgactcg agaatcaccg aaacctcaat
    13681 catgttgcca cccaagcagt tcctgccgat gtttcataca agattctttc accacaacga
    13741 ttggtcaaca gatctatttg ttcaagaaag gttttggcag aacgttgatc gagtgtatgc
    13801 caattacaac tctactctag acgaaccatg ggcaattgtt ttcaatacaa ttatactact
    13861 ttctatgagc gcagaaaact ctctccagac tcatgctccc ttggcaactt cgcaatttgc
    13921 tgcccccttc ttttcagcgc tcaaagttgc cctcaataaa gtccatttat taatggcgcc
    13981 gcgactcata aacgtacaag ccttggccct tctaagctta gctgccgagc aatttcatcc
    14041 gttcggagtt gcagaagcag tgtttgtgca agcctgtaat ctggcaagac gaatgggtct
    14101 taatcagatt gcgatgactg gaacatcgga tgcaattgaa gaggaagagc gtctcaaagt
    14161 ctcaagggct ttatacattc gtgacaaaag cctgggtatt tcacgaggca ccatatgctg
    14221 gctgccaggg tttgaagtgc aaaatatgaa actggacgac tttgaatcag ccgaaggaag
    14281 ataccatgct gcacgagtca cgattactgc ttttcaggag cgcgtgtatc gcctccattc
    14341 ttcatctggg gctcaagatg actttgctaa caaagcgggc ttccatccct ctggtataca
    14401 acacggtttg gccgatcttc tggaaattgg cacgatgtgc ggtccccacc ttgaactttg
    14461 gttagaattc ctcgctaccc gtattgttgc tctacgaagg aacactaaag cgcattacgt
    14521 caagcgcact ttaaaagatt gtagagcatg ttgcatcctg ctactattgg cattcggaaa
    14581 gggtgacaaa gcactgaggg aacgactcga gcaccattgc gacctccata caacttctca
    14641 acaaggttcc aaatcatcct ctctagactc tgaaatcagc ctcaatgagc acgcaatatc
    14701 gacgccaaga actctcctct cactcttccc ggtgtctagc ttatttctgt tggctgcaaa
    14761 cgtgatcaac cctgcaactc cagaggagga agcgaacact gatgatgact acgagcttct
    14821 cgtcagagtt tgcgattgct acgaggaagc tgatgcaagc ctacaagcca tcaacagggc
    14881 ccatcaagtt ggtaaggcgc tccaaagaat tctgtccatc attcaacacg tgcgtgggat
    14941 gaatggcact tcccctagca aaagcacgag tggttcgggt agcagccgat gtcactccgc
    15001 gtcgttgtca tccagtcagc tggaacaagg cggttctttt ccagatcagt cgagtttgca
    15061 gcatctgcca agttctggtt tcataaactc gcagcatttc acagatgggg cgccgctatc
    15121 ttcactctgt gtgaacgata ttgagacatt cgtaatgaac acgccatcag gacatgagat
    15181 aagctcggcg ttctccgtgg aaaatgatat gatgagcttc atgaggcaag atgctatgac
    15241 ttcgaatcat ttcagatcgg acaattacgg aatggaaatt tgttatccag tggagggtgc
    15301 ggacccaaat ggcgggttgc cgggaatatg aacaggcccc ttttcctttg tcgatatcat
    15361 gtaattagtt atgtcacgct tacattcacg ccctcctccc acatccgctc taaccgaaaa
    15421 ggaaggagtt agacaacctg aagtctaggt ccctatttat tttttttaat agttatgtta
    15481 gtattaagaa cgttatttat atttcaaatt tttctttttt ttctgtacaa acgcgtgtac
    15541 gcatgtaaca ttatactgaa aaccttgctt gagaaggttt tgggacgctc gaaggcttta
    15601 atttgcattt attgaaaagt aaatatctcg taacccggat gctttgggcg gtcgggtttt
    15661 gctactcgtc atccgatgag aaaaactgtt cccttttgcc ccaggtttcc attcatccga
    15721 gcgatcactt atctgacttc gtcacttttt catttcatcc gaaacaatca aaactgaagc
    15781 caatcaccac aaaattaaca ctcaacgtca tctttcacta ccctttacag aagaaaatat
    15841 ccatagtccg gactagcatc ccagtatgtg actcaatatt ggtgcaaaag agaaaagcat
    15901 aagtcagtcc aaagtccgcc cttaaccagg cacatcggaa ttcacaaaac gtttctttat
    15961 tatataaagg agctgcttca ctggcaaaat tcttattatt tgtcttggct tgctaatttc
    16021 atcttatcct ttttttcttt tcacacccaa atacctaaca attgagagaa aactcttagc
    16081 ataacataac aaaaagtcaa cgaaaaatgg acgcttacca tctggatcct ccgaaagttg
    16141 catcagcgca aacggcggca tctcctgaag agaaggccat accacagccc aggccgggac
    16201 ctactacgac tcccccagtt cttgatacgg caacactcaa gtacatcaaa acactaagta
    16261 tcaaacattt ccgttctgca gatgtcccat ggcgctccat gggtcagtac aagtaccgca
    16321 ttatcgagga tggcactctt acccaagacc gcatatccgt ggtagagtcg cttatcccgc
    16381 caaaatcaca cggtcctatt tttcatttcc atgagatgca cgacgaatgc ttctacgtca
    16441 cacaaggcac tgtgcgtttt cattcgccag gaagcccgcc agtggacgct catgtggggg
    16501 acatggtaac agtgccaacg aggatgcctc atcgattcag caacccattt gatgaggagg
    16561 ccaaatttgt atcaacaaac acaccgagtc actatgcacg ttacttctac tacctggaat
    16621 acctgattgg tgagggaaat gagctgacac cagaagtgaa taaagaagca atgcgtcggt
    16681 tcgctacggt gccattatcg cccgcggaca ttgaattgtt ggaaagagaa ttgggcgccg
    16741 gcacagttgg aaatgagaac gagtacaatt cagacgagtc agtagatacg gcggacttgg
    16801 tcagtcttga ttagggagat tgataagact tttctagttg catatctttt atatttaaat
    16861 cttatctatt agttaatttt ttgtaattta tccttatata tagtctggtt attctaaaat
    16921 atcatttcag tatctaaaaa ttcccctctt ttttcagtta tatcttaaca ggcgacagtc
    16981 caaatgttga tttatcccag tccgattcat cagggttgtg aagcattttg tcaatggtcg
    17041 aaatcacatc agtaatagtg cctcttactt gcctcataga atttctttct cttaacgtca
    17101 ccgtttggtc ttttatagtt tcgaaatcta tggtgatacc aaatggtgtt cccaattcat
    17161 cgttacgggc gtatttttta ccaattgaag tattggaatc gtcaatttta aagtatatct
    17221 ctcttttacg taaagcctgc gagatcctct taagtatagc ggggaagcca tcgttattcg
    17281 atattgtcgt aacaaatact ttgatcggcg ctatgcggcc gccaccgcgg tggagctcca
    17341 gcttttgttc cctttagtga gggttaattg cgcgcttggc gagactcaaa caagatatat
    17401 tttgcccgcc ttacagacga aactagttgg aggttatgga gcatactatc acgtgggcgg
    17461 ccactggtga gttactacac cccaggggca acgttgatgc tcctaaaaaa ctctggctgg
    17521 acgcaagccg taacacccgt gtcacttcat aatcgtttgc aattcagggc ttgatctaca
    17581 cattaccctg ttatccctat taattaatac ggttatccac agaatcaggg gataacgcag
    17641 gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc
    17701 tggcgttttt ccataggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc
    17761 agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc
    17821 tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt
    17881 cgggaagcgt ggcgctttct catagctcac gctgtaggta tctcagttcg gtgtaggtcg
    17941 ttcgctccaa gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat
    18001 ccggtaacta tcgtcttgag tccaacccgg taagacacga cttatcgcca ctggcagcag
    18061 ccactggtaa caggattagc agagcgaggt atgtaggcgg tgctacagag ttcttgaagt
    18121 ggtggcctaa ctacggctac actagaagga cagtatttgg tatctgcgct ctgctgaagc
    18181 cagttacctt cggaaaaaga gttggtagct cttgatccgg caaacaaacc accgctggta
    18241 gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag
    18301 atcctttgat cttttctacg gggtctgacg ctcagtggaa cgaaaactca cgttaaggga
    18361 ttttggtcat gagattatca aaaaggatct tcacctagat ccttttaaat taaaaatgaa
    18421 gttttaaatc aatctaaagt atatatgagt aaacttggtc tgacagttac caatgcttaa
    18481 tcagtgaggc acctatctca gcgatctgtc tatttcgttc atccatagtt gcctgactcc
    18541 ccgtcgtgta gataactacg atacgggagg gcttaccatc tggccccagt gctgcaatga
    18601 taccgcgaga cccacgctca ccggctccag atttatcagc aataaaccag ccagccggaa
    18661 gggccgagcg cagaagtggt cctgcaactt tatccgcctc catccagtct attaattgtt
    18721 gccgggaagc tagagtaagt agttcgccag ttaatagttt gcgcaacgtt gttgccattg
    18781 ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc tccggttccc
    18841 aacgatcaag gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt agctccttcg
    18901 gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg gttatggcag
    18961 cactgcataa ttctcttact gtcatgccat ccgtaagatg cttttctgtg actggtgagt
    19021 actcaaccaa gtcattctga gaatagtgta tgcggcgacc gagttgctct tgcccggcgt
    19081 caatacggga taataccgcg ccacatagca gaactttaaa agtgctcatc attggaaaac
    19141 gttcttcggg gcgaaaactc tcaaggatct taccgctgtt gagatccagt tcgatgtaac
    19201 ccactcgtgc acccaactga tcttcagcat cttttacttt caccagcgtt tctgggtgag
    19261 caaaaacagg aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa
    19321 tactcatact cttccttttt caatattatt gaagcattta tcagggttat tgtctcatga
    19381 gcggatacat atttgaatgt atttagaaaa ataaacaaat aggggttccg cgcacatttc
    19441 cccgaaaagt gccacctgaa cgaagcatct gtgcttcatt ttgtagaaca aaaatgcaac
    19501 gcgagagcgc taatttttca aacaaagaat ctgagctgca tttttacaga acagaaatgc
    19561 aacgcgaaag cgctatttta ccaacgaaga atctgtgctt catttttgta aaacaaaaat
    19621 gcaacgcgag agcgctaatt tttcaaacaa agaatctgag ctgcattttt acagaacaga
    19681 aatgcaacgc gagagcgcta ttttaccaac aaagaatcta tacttctttt ttgttctaca
    19741 aaaatgcatc ccgagagcgc tatttttcta acaaagcatc ttagattact ttttttctcc
    19801 tttgtgcgct ctataatgca gtctcttgat aactttttgc actgtaggtc cgttaaggtt
    19861 agaagaaggc tactttggtg tctattttct cttccataaa aaaagcctga ctccacttcc
    19921 cgcgtttact gattactagc gaagctgcgg gtgcattttt tcaagataaa ggcatccccg
    19981 attatattct ataccgatgt ggattgcgca tactttgtga acagaaagtg atagcgttga
    20041 tgattcttca ttggtcagaa aattatgaac ggtttcttct attttgtctc tatatactac
    20101 gtataggaaa tgtttacatt ttcgtattgt tttcgattca ctctatgaat agttcttact
    20161 acaatttttt tgtctaaaga gtaatactag agataaacat aaaaaatgta gaggtcgagt
    20221 ttagatgcaa gttcaaggag cgaaaggtgg atgggtaggt tatataggga tatagcacag
    20281 agatatatag caaagagata cttttgagca atgtttgtgg aagcggtatt cgcaatattt
    20341 tagtagctcg ttacagtccg gtgcgttttt ggttttttga aagtgcgtct tcagagcgct
    20401 tttggttttc aaaagcgctc tgaagttcct atactttcta gagaatagga acttcggaat
    20461 aggaacttca aagcgtttcc gaaaacgagc gcttccgaaa atgcaacgcg agctgcgcac
    20521 atacagctca ctgttcacgt cgcacctata tctgcgtgtt gcctgtatat atatatacat
    20581 gagaagaacg gcatagtgcg tgtttatgct taaatgcgta cttatatgcg tctatttatg
    20641 taggatgaaa ggtagtctag tacctcctgt gatattatcc cattccatgc ggggtatcgt
    20701 atgcttcctt cagcactacc ctttagctgt tctatatgct gccactcctc aattggatta
    20761 gtctcatcct tcaatgctat catttccttt gatattggat catactaaga aaccattatt
    20821 atcatgacat taacctataa aaataggcgt atcacgaggc cctttcgtct cgcgcgtttc
    20881 ggtgatgacg gtgaaaacct cggcgcgcca ttaccctgtt atccctaata tacaacgcaa
    20941 agaagctgga aaattggcga gggaatcctg tttctgtcta tccaagaatg ggcatgaggt
    21001 ggcaaccgtc gtgctagcgt acagggtgca ctttgtaacc atttgggaca ccggacactc
    21061 gctgttttcg aaattaccct ttaagcgcgg gtattgaacc aggcttatgc ccaagatcgt
    21121 agcaagcatt taaatgacgg tatcgataag cttgatatcg aattcctgca gcccggggga
    21181 tccactagtt ctagagttaa ttaa
//
