LOCUS       sequence-208194-       13241 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of a fungal biosynthetic gene cluster.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13241)
  AUTHORS   Harvey CJB, Tang M, Schlecht U, Horecka J, Fischer CR, Lin HC, Li J,
            Naughton B, Cherry J, Miranda M, Li YF, Chu AM, Hennessy JR, Vandova
            GA, Inglis D, Aiyar RS, Steinmetz LM, Davis RW, Medema MH, Sattely 
            E, Khosla C, St Onge RP, Tang Y, Hillenmeyer ME
  TITLE     HEx: A heterologous expression platform for the discovery of fungal 
            natural products.
  JOURNAL   Sci Adv. 2018 Apr 11;4(4):eaar5459. doi: 10.1126/sciadv.aar5459. 
            eCollection 2018 Apr.
  PUBMED    29651464
REFERENCE   2  (bases 1 to 13241)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Adv."; 
            date: "2018-04-11"; volume: "4(4):eaar5459. doi"; pages: " 
            10.1126/sciadv.aar5459. eCollection 2018 Apr"
COMMENT     Sequence Label: pCHKU39.1-2.2
FEATURES             Location/Qualifiers
     source          1..13241
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             504..527
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     primer_bind     complement(7676..7696)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(7676..7694)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     rep_origin      complement(8033..8621)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8113..8132)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8792..9583,9584..9652))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9415..9434
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9653..9757)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      9784..11126
                     /label=2u ori
                     /note="yeast 2u plasmid origin of replication"
     protein_bind    complement(10747..10794)
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     primer_bind     11167..11185
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        11457..11861
                     /gene="S. cerevisiae LEU2"
                     /label=LEU2 promoter
     CDS             11874..12968
                     /codon_start=1
                     /gene="S. cerevisiae LEU2"
                     /product="3-isopropylmalate dehydrogenase, required for 
                     leucine biosynthesis"
                     /label=LEU2
                     /note="yeast auxotrophic marker"
                     /translation="MSAPKKIVVLPGDHVGQEITAEAIKVLKAISDVRSNVKFDFENHL
                     IGGAAIDATGVPLPDEALEASKKVDAVLLGAVAGPKWGTGSVRPEQGLLKIRKELQLYA
                     NLRPCNFASDSLLDLSPIKPQFAKGTDFVVVRELVGGIYFGKRKEDDGDGVAWDSEQYT
                     VPEVQRITRMAAFMALQHEPPLPIWSLDKANLLASSRLWRKTVEETIKNEFPTLKVQHQ
                     LIDSAAMILVKNPTHLNGIIITSNMFGDIISDEASVIPGSLGLLPSASLASLPDKNTAF
                     GLYEPCHGSAPDLPKNKVDPIATILSAAMMLKLSLNLPEEGKAIEDAVKKVLDAGIRTG
                     DLGGSNSTTEVGDAVAEEVKKILA"
     primer_bind     complement(13216..13232)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(13216..13232)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
ORIGIN
        1 tatctaaaaa ttgccttatg atccgtctct ccggttacag cctgtgtaac tgattaatcc
       61 tgcctttcta atcaccattc taatgtttta attaagggat tttgtcttca ttaacggctt
      121 tcgctcataa aaatgttatg acgttttgcc cgcaggcggg aaaccatcca cttcacgaga
      181 ctgatctcct ctgccggaac accgggcatc tccaacttat aagttggaga aataagagaa
      241 tttcagattg agagaatgaa aaaaaaaaaa aaaaaaaagg cagaggagag catagaaatg
      301 gggttcactt tttggtaaag ctatagcatg cctatcacat ataaatagag tgccagtagc
      361 gacttttttc acactcgaaa tactcttact actgctctct tgttgttttt atcacttctt
      421 gtttcttctt ggtaaataga atatcaagct acaaaaagca tacaatcaac tatcaactat
      481 taactatatc gtaatacaca atggattata aggatgatga tgataagact agtatggcac
      541 ctacgaccac attcatcaat gttcctgtct tcgccggtca cggtacgact gccatggctg
      601 cgtcttccac tcgtgaacag gccattgctg atgcttctca cccatctggc gctttgcttc
      661 tcagctcgtt ccatcgtgct ttcctccgcg aacgagcttc gctctcatcc gaagatcttg
      721 acgatttagc tctccccgac ttttctactc cccaagaatt cctctccatc atcggcgaac
      781 agcccattag taactccctt caatccaacc tttctcttct cctcgtccag gcgcttagat
      841 atctcgccca cgtggaggca ggctctttat cggagtcact taaccctttc accgaattct
      901 tggtcagcaa tgtagatcat aaagtcggcg tggctgggtt ctcctcggga atcctgcctg
      961 cctgtgcagt ggcatgctct caggacagcc tatcctttat caaacatgcc gtagagcttt
     1021 tccgctttgc tttctggttg ggacttagat cccaacagta ccagaccaaa gcgactcgtg
     1081 aactgaccaa atctcatgga gaaaaaacgc gtcagttttg gagtagagtt atcatgggac
     1141 aatctgagtc tgaagttaga gctgcaatag acatcttcgc tgtacagaac cccacgcttc
     1201 cgcaaattta tatcaccgct atcagtgatg acacgacgtt cacggtctct ggtcgcccgg
     1261 acgctttatc tgcattcata gaaacccttc cttctacaag ccgaatattt aatttgacgg
     1321 tggatacatt atatcactct ccttgccacc aggacggatt gcgagcgcag gttctcgcag
     1381 atgtcacccg acgtggcgta atattcccta ccctagataa cctaatcttc cctctccgat
     1441 caacttctac tggtgaactc gtaaacgagt ctaacgacgg cacaactcta ttagaaaaca
     1501 tcctcgatat gatcgttgtt caaccagtca agtggcactt ggtcacagac gctttgtcca
     1561 aggcgccccc agtcgacatt cctattcgct tgttgaactt tggacccgga acaggactca
     1621 ctcgttccat ggttaaagca ttttccaagg acatcgtttc cactcaagac ttgacctcgg
     1681 agaatgcggc caagcgccta gaaggtgcta cgaccgaagg acagatcccc attgccattg
     1741 tgggaatggc attgaacatg cctggagctc cgagtgccgc caagttgtgg gagttgctcg
     1801 aaaacggtat caacaccgtc tcggaggttc cgtccgaacg cttcaacatc tcggagtaca
     1861 acaagccttc ggatcccaaa tctaggcgag ccatgaaggc gcacaccgga aacttcatgg
     1921 cagacccaag cctctttgat gccaagttct tccgtatttc tcctcgtgaa gcaaagagca
     1981 tggaccccca gcagcgtatc ttgcttcaga ccgcttacga ggctttggag aatgccggat
     2041 acgtcccgaa tgctacacca accttccaac aggacacctt tggttgttac gtcggtgttg
     2101 cgactgatga ctacgttcag aatctcagag acgagattga tgtctactat agtactggta
     2161 cccttcgtgc gttccttagt gggcgtattt catacgcaat gggattcagc gggccctcaa
     2221 tcgtcatcga cacagcctgc tcatcatctt gtgtttctgt ttaccaagct tgtcgggcgt
     2281 tatcgaacgg agactgtaac gcagctgtcg ctggtggtgt taatgtcatt gcaagcccgg
     2341 atatgatgat cggcttggac agggcccatt ttttgagtcc caccggccag tgcaagccat
     2401 ttgacgcttc tgccgacggc tactctcgct ctgaaggatg cggattgttc gttttgaaac
     2461 gcctctctga cgccgtcgcc gagaacgata acatcctcgg ggtaattcgt ggggttgagg
     2521 tcaaccaatc aggaaatgct cattcgatta ctcaccccca tgcacctacc caagtcaagc
     2581 tgttcgagag gttgcttgag aaaactggcg ttgataggca tcgtatcaac gtgatcgagg
     2641 cacatggtac tggtactcag gccggagatc caaatgagct tgaaagtatt cgcaagactt
     2701 ttgctacggg tcgtccaaag tcgaaccctc tgcatatcac ttccgtcaag gccaacattg
     2761 ggcacctcga agcagcaagt ggttcggctg gtttggccaa gctccttctc atgcttcgcc
     2821 accgcaccat cccccggttg atctctttga agaatctcaa cccactcatt gctccgttgg
     2881 acttggataa cactgcgatt gatacagcag cctgcgaatg ggctccttca gagcccggtc
     2941 tgcctcgact cgccatgctc aacaactttg gtgctgccgg ttccaatgga gctgtactgc
     3001 tggaggaata tgtagcgcct cctcgtgacg accccataac tgcgccgatt actttaccct
     3061 ttggtttatc tgccaaagat tccattgcgc tcgatcaact gcgccagcgc tatgtggact
     3121 accttaggct tcccgagaac gaaggaacga gcctttacga cattgcttat accatgactg
     3181 cgcgtcgcca gatgtaccca ttccgtatgg ctgttacagc gtcttctcgc caggagctcg
     3241 tcgagaagct tctacaggcc tctggcactc aagcaaaaga gtcgaacgcc gaagtagctt
     3301 tcgtattctc cggccagggt ggtcaatact tgggcatggg tgcggctttg tatgaaacct
     3361 gccctgtctt caagacccac gttgacgaat gtcgctcatt actatcctgt atgggcttcg
     3421 gcgacatcct gtcaatcatt tcgccatccg gtgagagaag tgggctaagt gctacagatg
     3481 aattggaggt ttaccaaact gctgtgtttg ccttggaata ttccctggca caactatgga
     3541 tgtcctgggg cctttctccg gtcgcagttg ttggtcacag cctgggagaa tacgctgcct
     3601 tggttattgc aggagtcctc agtcttcgga gcgctctatt tgtgatcgca tcccgagtgc
     3661 gtctcatgct tcgcaagtgc gagatgaata gcacagggat gatagcaatc aataacggcc
     3721 ccgctgaagt ccaaaagata ctcgattcaa ctcctttgtt tgaatctctc agtgtcgcct
     3781 gctacaacag cgtctcggac tgcgtcgttg ctggtccttt gacaggattg aaggcactca
     3841 agtcttattt ggacgatgag atccgctgca agagtaccat cttgaacgtt ccatttggat
     3901 accattccgc tgcaatgaac ccgctggtgg atgatttgaa cgcggtcttg gaaaccgtca
     3961 aacttcatcc cccgaggatt cctgtagtat ccaacgtttt cggcagtgta gtagagccag
     4021 gagatggtac cgttttcact tcgacgtact tctcgcgtca ctgtgccgag cctgtcaaat
     4081 tctcagatgg gtttgccaca cttctggccg acgcggaact tgctgccaat gtttggattg
     4141 aggtcgggcc tcatccgaca accctaccca tgatgaaagc atgccccgct ctgccaaagg
     4201 atattcgcct tcttccttcg ctcaggaaga accaagaccc ttggagtact ctcaccacct
     4261 cattgagtac cctgtatgca gaaaagtcgg atgtcgcttg gaggaatgtg ttcagtcacc
     4321 ttggcagtgt cagctgtgtc gatttgccga cgtacccgtt cactaacgtg aaatattggg
     4381 tcaagttcga ggagagtgaa gccactgtgc atacagccac accccttcca gcgcaacagt
     4441 cggtcgatac gatcaatcaa ttctcacttt tgcgttcctg ggttcagcgc ccatcaactg
     4501 cgaatgggaa cgtggcaatt gtggaggttc ccatcagcgt cctctccaag ctcattagcg
     4561 gccatcaagt tggtgaacat cctctgtgtc ccgcatcggt ttaccatgaa ctcgcaatgg
     4621 cagctatcaa acttgcgacg acgaataaac caggacatta catcgcctta cgggatattg
     4681 actatgctaa gcctttggtt catgctgaga acgacaccag gaatatcagg accgttgtct
     4741 ctatccctga tggtagttgg aaggtcatct cgcacagcag cgacgaaagc gtacactgca
     4801 ccggaaagtt ctcctgggat aacgtcggaa aagctgccct caaattccag cagtatagac
     4861 ccattctcag ccgggaaatc gcctctgtca ttgacaatgt agattccgaa cttttctcga
     4921 ctcgtacgat gtacgacatc atcttcccgc gtgtcgttca ctattccaaa gagtatcact
     4981 ccgcacgatg ccttactgtt cacccttctg gtatggaggg tcatgctacc gtcaaaatcc
     5041 cacacgcgtc aacccccggt cagtacgtcg ttcatccggt cttcatggac accctccttc
     5101 acgttgcagg tttcgttgcg aatatgcgag ccggtgccaa tgacgctttc atctgcagcc
     5161 aggtggactc tgtcaaggtg gtccctgaac tggtcaataa tgatgcgacg tacgatgtct
     5221 tctgccggaa tgcatggctt cctgaggagg gactcattct tgcggatgcc tatgccgtca
     5281 ccacgggtac ctctccgaaa attgttgccc atttgaagcg tatgcatttc cgcaaagtcc
     5341 ggctcgctag tctcaagcga gctttgaaac atgctcaagg cggatccacc gctccgactc
     5401 gcaccgccgc aaagtctgca tcatcggttg ttgctgcaaa gacatcagca tcgcccgccc
     5461 aagtcaaagt tcgaggcaag ggcgaggata tactagatca agtccgaaaa gccgtcgcag
     5521 atacctgtga cgtcccgatg acttcaatgg acatttctgc tgatctcacc tcctttggtg
     5581 tcgattcttt gatgagtatc gagatatttg gtcggcttca agcgttgttc ccagatgcag
     5641 aactagatcc acgagtccta tctcatctcc gtaccatttc ggaaatagca gcagaagtgg
     5701 cgtcgaaggc tggtgatctt tcaccagccg ctacctctga ggcagagtcg tcatctcctg
     5761 ctccatcgac tccctccaac tgcgggggca atgttttaga tcaggttcgt caggcagttg
     5821 cggacacttg tgacctccca gtgacctcga tgaacatctc tgctgatctc acttcccttg
     5881 gagttgactc cctgatgagt atcgagatat ttggtcgact tcaaatgctg ttcccagatg
     5941 cagatctaga cccacggatc ctatcccatc tccataccat ttcagaaatc acagccgagg
     6001 tgacgtccaa ggtcggggat gcttcgctgg ctactacccc tgagtcttcg ggaacagctg
     6061 ttgaggatgt cgttgcagat actatggtca tggaagagca aggagccgat gtgaagacgg
     6121 taatagcagg ggtgcttggt atcggcgccg acgaattcaa cgacgacact gacttggagt
     6181 cgctcggtct ggattcactg acatctattg aagttatgca agcagtgcag gacaacctct
     6241 ccatctctat tcctcaacac cttttggcct ctcatcccac agttcgagca atcaacgaat
     6301 ttgtttctag tcaacggaag ccgccaaaaa ccgaagcaca aagtggtaac gtcgccaagg
     6361 ctccagtgct ttctgccgcc gccttgaaag ctcttgcgct cgatgaaatg ccagccccgc
     6421 ttatacgaga tccaacatcc cagaggaccc ctcttttcct gattcatgat ggaagcggct
     6481 tggccaacta ctacggtcgg atgtcaagtg ttcagcgcaa tgtttggggc attttcaatc
     6541 ccaattttgc gacatcggaa ccttggacag acttgaagac gatggctgcc aagtatgcgt
     6601 ctataatttc cagcacggca cgcgggccag ctatcatcgg aggttggtct ttcggtggtg
     6661 tggtagcttt cgaagcggct cgccaactga tgtcggccgg ccattctgtc aaaggggtcg
     6721 tcctcatcga ttcgcccagt ccagtcaatc acgtacctct ctcggacgct gtgatcgact
     6781 cggttgtgaa tttagacagt cgtgcagcca agtcagacat cagtcgatta gtcaaacgac
     6841 aattccagat gaattccgca atgttaggga agtatgaacc atcgacaggt ggccattatc
     6901 cccctctcgt tctcctcaga tcttcggagg gctaccaacg ccccaacgtg tctgttccga
     6961 tttggttgag cgaccgaagc gacccccgac aagccgtcag tggatggcaa caggttgttg
     7021 gtactgaggt tagagtgatg gatattacag gaaaccactt cgagccattt agccctgcac
     7081 atatcccctc ggtttctcaa agagtttcag aagcatgtag ttatcttgaa tctctctaag
     7141 gagattgata agacttttct agttgcatat cttttatatt taaatcttat ctattagtta
     7201 attttttgta atttatcctt atatatagtc tggttattct aaaatatcat ttcagtatct
     7261 aaaaattccc ctcttttttc agttatatct taacaggcga cagtccaaat gttgatttat
     7321 cccagtccga ttcatcaggg ttgtgaagca ttttgtcaat ggtcgaaatc acatcagtaa
     7381 tagtgcctct tacttgcctc atagaatttc tttctcttaa cgtcaccgtt tggtctttta
     7441 tagtttcgaa atctatggtg ataccaaatg gtgttcccaa ttcatcgtta cgggcgtatt
     7501 ttttaccaat tgaagtattg gaatcgtcaa ttttaaagta tatctctctt ttacgtaaag
     7561 cctgcgagat cctcttaagt atagcgggga agccatcgtt attcgatatt gtcgtaacaa
     7621 atactttgat cggcgctatg cggccgccac cgcggtggag ctccagcttt tgttcccttt
     7681 agtgagggtt aattgcgcgc ttggcgagac tcaaacaaga tatattttgc ccgccttaca
     7741 gacgaaacta gttggaggtt atggagcata ctatcacgtg ggcggccact ggtgagttac
     7801 tacaccccag gggcaacgtt gatgctccta aaaaactctg gctggacgca agccgtaaca
     7861 cccgtgtcac ttcataatcg tttgcaattc agggcttgat ctacacatta ccctgttatc
     7921 cctattaatt aatacggtta tccacagaat caggggataa cgcaggaaag aacatgtgag
     7981 caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata
     8041 ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc
     8101 cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg
     8161 ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc
     8221 tttctcatag ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg
     8281 gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc
     8341 ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga
     8401 ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg
     8461 gctacactag aaggacagta tttggtatct gcgctctgct gaagccagtt accttcggaa
     8521 aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg
     8581 tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt
     8641 ctacggggtc tgacgctcag tggaacgaaa actcacgtta agggattttg gtcatgagat
     8701 tatcaaaaag gatcttcacc tagatccttt taaattaaaa atgaagtttt aaatcaatct
     8761 aaagtatata tgagtaaact tggtctgaca gttaccaatg cttaatcagt gaggcaccta
     8821 tctcagcgat ctgtctattt cgttcatcca tagttgcctg actccccgtc gtgtagataa
     8881 ctacgatacg ggagggctta ccatctggcc ccagtgctgc aatgataccg cgagacccac
     8941 gctcaccggc tccagattta tcagcaataa accagccagc cggaagggcc gagcgcagaa
     9001 gtggtcctgc aactttatcc gcctccatcc agtctattaa ttgttgccgg gaagctagag
     9061 taagtagttc gccagttaat agtttgcgca acgttgttgc cattgctaca ggcatcgtgg
     9121 tgtcacgctc gtcgtttggt atggcttcat tcagctccgg ttcccaacga tcaaggcgag
     9181 ttacatgatc ccccatgttg tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg
     9241 tcagaagtaa gttggccgca gtgttatcac tcatggttat ggcagcactg cataattctc
     9301 ttactgtcat gccatccgta agatgctttt ctgtgactgg tgagtactca accaagtcat
     9361 tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata cgggataata
     9421 ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa
     9481 aactctcaag gatcttaccg ctgttgagat ccagttcgat gtaacccact cgtgcaccca
     9541 actgatcttc agcatctttt actttcacca gcgtttctgg gtgagcaaaa acaggaaggc
     9601 aaaatgccgc aaaaaaggga ataagggcga cacggaaatg ttgaatactc atactcttcc
     9661 tttttcaata ttattgaagc atttatcagg gttattgtct catgagcgga tacatatttg
     9721 aatgtattta gaaaaataaa caaatagggg ttccgcgcac atttccccga aaagtgccac
     9781 ctgaacgaag catctgtgct tcattttgta gaacaaaaat gcaacgcgag agcgctaatt
     9841 tttcaaacaa agaatctgag ctgcattttt acagaacaga aatgcaacgc gaaagcgcta
     9901 ttttaccaac gaagaatctg tgcttcattt ttgtaaaaca aaaatgcaac gcgagagcgc
     9961 taatttttca aacaaagaat ctgagctgca tttttacaga acagaaatgc aacgcgagag
    10021 cgctatttta ccaacaaaga atctatactt cttttttgtt ctacaaaaat gcatcccgag
    10081 agcgctattt ttctaacaaa gcatcttaga ttactttttt tctcctttgt gcgctctata
    10141 atgcagtctc ttgataactt tttgcactgt aggtccgtta aggttagaag aaggctactt
    10201 tggtgtctat tttctcttcc ataaaaaaag cctgactcca cttcccgcgt ttactgatta
    10261 ctagcgaagc tgcgggtgca ttttttcaag ataaaggcat ccccgattat attctatacc
    10321 gatgtggatt gcgcatactt tgtgaacaga aagtgatagc gttgatgatt cttcattggt
    10381 cagaaaatta tgaacggttt cttctatttt gtctctatat actacgtata ggaaatgttt
    10441 acattttcgt attgttttcg attcactcta tgaatagttc ttactacaat ttttttgtct
    10501 aaagagtaat actagagata aacataaaaa atgtagaggt cgagtttaga tgcaagttca
    10561 aggagcgaaa ggtggatggg taggttatat agggatatag cacagagata tatagcaaag
    10621 agatactttt gagcaatgtt tgtggaagcg gtattcgcaa tattttagta gctcgttaca
    10681 gtccggtgcg tttttggttt tttgaaagtg cgtcttcaga gcgcttttgg ttttcaaaag
    10741 cgctctgaag ttcctatact ttctagagaa taggaacttc ggaataggaa cttcaaagcg
    10801 tttccgaaaa cgagcgcttc cgaaaatgca acgcgagctg cgcacataca gctcactgtt
    10861 cacgtcgcac ctatatctgc gtgttgcctg tatatatata tacatgagaa gaacggcata
    10921 gtgcgtgttt atgcttaaat gcgtacttat atgcgtctat ttatgtagga tgaaaggtag
    10981 tctagtacct cctgtgatat tatcccattc catgcggggt atcgtatgct tccttcagca
    11041 ctacccttta gctgttctat atgctgccac tcctcaattg gattagtctc atccttcaat
    11101 gctatcattt cctttgatat tggatcatac taagaaacca ttattatcat gacattaacc
    11161 tataaaaata ggcgtatcac gaggcccttt cgtctcgcgc gtttcggtga tgacggtgaa
    11221 aacctcggcg cgccattacc ctgttatccc taatatacaa cgcaaagaag ctggaaaatt
    11281 ggcgagggaa tcctgtttct gtctatccaa gaatgggcat gaggtggcaa ccgtcgtgct
    11341 agcgtacagg gtgcactttg taaccatttg ggacaccgga cactcgctgt tttcgaaatt
    11401 accctttaag cgcgggtatt gaaccaggct tatgcccaag atcgtagcaa gcatttaact
    11461 gtgggaatac tcaggtatcg taagatgcaa gagttcgaat ctcttagcaa ccattatttt
    11521 tttcctcaac ataacgagaa cacacagggg cgctatcgca cagaatcaaa ttcgatgact
    11581 ggaaattttt tgttaatttc agaggtcgcc tgacgcatat acctttttca actgaaaaat
    11641 tgggagaaaa aggaaaggtg agaggccgga accggctttt catatagaat agagaagcgt
    11701 tcatgactaa atgcttgcat cacaatactt gaagttgaca atattattta aggacctatt
    11761 gttttttcca ataggtggtt agcaatcgtc ttactttcta acttttctta ccttttacat
    11821 ttcagcaata tatatatata tttcaaggat ataccattct aatgtctgcc cctatgtctg
    11881 cccctaagaa gatcgtcgtt ttgccaggtg accacgttgg tcaagaaatc acagccgaag
    11941 ccattaaggt tcttaaagct atttctgatg ttcgttccaa tgtcaagttc gatttcgaaa
    12001 atcatttaat tggtggtgct gctatcgatg ctacaggtgt cccacttcca gatgaggcgc
    12061 tggaagcctc caagaaggtt gatgccgttt tgttaggtgc tgtggctggt cctaaatggg
    12121 gtaccggtag tgttagacct gaacaaggtt tactaaaaat ccgtaaagaa cttcaattgt
    12181 acgccaactt aagaccatgt aactttgcat ccgactctct tttagactta tctccaatca
    12241 agccacaatt tgctaaaggt actgacttcg ttgttgtcag agaattagtg ggaggtattt
    12301 actttggtaa gagaaaggaa gacgatggtg atggtgtcgc ttgggatagt gaacaataca
    12361 ccgttccaga agtgcaaaga atcacaagaa tggccgcttt catggcccta caacatgagc
    12421 caccattgcc tatttggtcc ttggataaag ctaatctttt ggcctcttca agattatgga
    12481 gaaaaactgt ggaggaaacc atcaagaacg aattccctac attgaaggtt caacatcaat
    12541 tgattgattc tgccgccatg atcctagtta agaacccaac ccacctaaat ggtattataa
    12601 tcaccagcaa catgtttggt gatatcatct ccgatgaagc ctccgttatc ccaggttcct
    12661 tgggtttgtt gccatctgcg tccttggcct ctttgccaga caagaacacc gcatttggtt
    12721 tgtacgaacc atgccacggt tctgctccag atttgccaaa gaataaggtt gaccctatcg
    12781 ccactatctt gtctgctgca atgatgttga aattgtcatt gaacttgcct gaagaaggta
    12841 aggccattga agatgcagtt aaaaaggttt tggatgcagg tatcagaact ggtgatttag
    12901 gtggttccaa cagtaccacc gaagtcggtg atgctgtcgc cgaagaagtt aagaaaatcc
    12961 ttgcttaaaa agattctctt tttttatgat atttgtacat aaactttata aatgaaattc
    13021 ataatagaaa cgacacgaaa ttacaaaatg gaatatgttc atagggtaga cgaaactata
    13081 tacgcaatct acatacattt atcaagaagg agaaaaagga ggatagtaaa ggaatacagg
    13141 taagcaaatt gatactaatg gctcaacgaa atgacggtat cgataagctt gatatcgaat
    13201 tcctgcagcc cgggggatcc actagttcta gagttaatta a
//
