LOCUS       sequence-208195-       13364 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of a fungal biosynthetic gene cluster.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13364)
  AUTHORS   Harvey CJB, Tang M, Schlecht U, Horecka J, Fischer CR, Lin HC, Li J,
            Naughton B, Cherry J, Miranda M, Li YF, Chu AM, Hennessy JR, Vandova
            GA, Inglis D, Aiyar RS, Steinmetz LM, Davis RW, Medema MH, Sattely 
            E, Khosla C, St Onge RP, Tang Y, Hillenmeyer ME
  TITLE     HEx: A heterologous expression platform for the discovery of fungal 
            natural products.
  JOURNAL   Sci Adv. 2018 Apr 11;4(4):eaar5459. doi: 10.1126/sciadv.aar5459. 
            eCollection 2018 Apr.
  PUBMED    29651464
REFERENCE   2  (bases 1 to 13364)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Adv."; 
            date: "2018-04-11"; volume: "4(4):eaar5459. doi"; pages: " 
            10.1126/sciadv.aar5459. eCollection 2018 Apr"
COMMENT     Sequence Label: pCHKU42.1-2.2
FEATURES             Location/Qualifiers
     source          1..13364
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             504..527
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     primer_bind     complement(7799..7819)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(7799..7817)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     rep_origin      complement(8156..8744)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8236..8255)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8915..9706,9707..9775))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9538..9557
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9776..9880)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      9907..11249
                     /label=2u ori
                     /note="yeast 2u plasmid origin of replication"
     protein_bind    complement(10870..10917)
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     primer_bind     11290..11308
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        11580..11984
                     /gene="S. cerevisiae LEU2"
                     /label=LEU2 promoter
     CDS             11997..13091
                     /codon_start=1
                     /gene="S. cerevisiae LEU2"
                     /product="3-isopropylmalate dehydrogenase, required for 
                     leucine biosynthesis"
                     /label=LEU2
                     /note="yeast auxotrophic marker"
                     /translation="MSAPKKIVVLPGDHVGQEITAEAIKVLKAISDVRSNVKFDFENHL
                     IGGAAIDATGVPLPDEALEASKKVDAVLLGAVAGPKWGTGSVRPEQGLLKIRKELQLYA
                     NLRPCNFASDSLLDLSPIKPQFAKGTDFVVVRELVGGIYFGKRKEDDGDGVAWDSEQYT
                     VPEVQRITRMAAFMALQHEPPLPIWSLDKANLLASSRLWRKTVEETIKNEFPTLKVQHQ
                     LIDSAAMILVKNPTHLNGIIITSNMFGDIISDEASVIPGSLGLLPSASLASLPDKNTAF
                     GLYEPCHGSAPDLPKNKVDPIATILSAAMMLKLSLNLPEEGKAIEDAVKKVLDAGIRTG
                     DLGGSNSTTEVGDAVAEEVKKILA"
     primer_bind     complement(13339..13355)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(13339..13355)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
ORIGIN
        1 tatctaaaaa ttgccttatg atccgtctct ccggttacag cctgtgtaac tgattaatcc
       61 tgcctttcta atcaccattc taatgtttta attaagggat tttgtcttca ttaacggctt
      121 tcgctcataa aaatgttatg acgttttgcc cgcaggcggg aaaccatcca cttcacgaga
      181 ctgatctcct ctgccggaac accgggcatc tccaacttat aagttggaga aataagagaa
      241 tttcagattg agagaatgaa aaaaaaaaaa aaaaaaaagg cagaggagag catagaaatg
      301 gggttcactt tttggtaaag ctatagcatg cctatcacat ataaatagag tgccagtagc
      361 gacttttttc acactcgaaa tactcttact actgctctct tgttgttttt atcacttctt
      421 gtttcttctt ggtaaataga atatcaagct acaaaaagca tacaatcaac tatcaactat
      481 taactatatc gtaatacaca atggattata aggatgatga tgataagact agtatgcgca
      541 ccgaaactgc ctgcccaatc gcaatcgttg gaatagcggc tgagttgccg agcggcgaat
      601 ttgctgcaaa caacctcgac tacaacgcgt tcttccaatt cctcctccag cgaggagagt
      661 catacgaatt catcccaaag gaccgcttcg acatagactc ctggagggga aagcagctgg
      721 gacgcatcat tgcggaacgc ggatccttcc tgaagaatct tgattcgttc gactacctcg
      781 aattcggcat aacgagcaag gatgctcgtg ctatggccgt cagcacccgg aagctcattg
      841 agctgtcctt cttagcactc ctggactccg gcatagagta ccgaggcagg aacgtcggct
      901 gttacgcctc cggtaatgca ttcgatatcc tgagcgtcgg taccgcggat gaattcgaag
      961 cgcgaggatc cttcgccgga atacctgctc agatcgcgaa tcggatttca taccacctgg
     1021 acctgttagg tccttcggtg ccgacagata ccgcatgcag ttcatctttg accgcgatgc
     1081 atcttgccat ccaagcactt cgcacggatg actgcgaagc tgcagtcgtg ggcggagccc
     1141 agatcaacca gagattgtgg gacttcgtcc agtatacaca gggcggtgta ctcgctcccg
     1201 acggcaagtg taagccgttc gacatgggag cagatgggtt ctctcgtggc gaaggtgctg
     1261 tagtaatggt catcaaaccg ctggaggcag cgcttcgcga cggtgacaac atatatggca
     1321 cgatcttggg caccggcatt aacagtgcag gatctgaggc tccggtgaac gcaccggcag
     1381 catcagctca agaagacact atgcgtcgtg cgttcaagca agcgaatcgc aaggtggaag
     1441 atctcgatta cgtcgagctg cacgcgaccg ggactgctaa gggagatccc acggaggcga
     1501 attgggtggg agccgccttc agacgagaag acgaacttct cgttgggagt gtgaaaggca
     1561 atataggcca tctcgagata accgctttcc ttgcgtcggt ctgcaaggtc tgcggtatat
     1621 ttaactccgg aatcattcct cccaacgtca gcctaacccg cctaaacccg gccataaaat
     1681 gggacgaata ccgtatgcgg gtgcctctcg agccaacacc gctgtcgtgc cgctcttcca
     1741 gcggtaagtc ccttgtttcg atcaacagct tcggcatagg aggctcgaat ggacactgcg
     1801 ttatggaatc tcctccggct tcactaccca agccggtttc gctggctgag ggcgcgcctg
     1861 ttctcttgct cgccggtgga ttatctccgc gtgcaactac tgcagtctcc gattctctca
     1921 gcgagctcgt ccgggaacgg atctctcaga tacccaacat ctccactctc tacgggcgac
     1981 gtagccgcca gatgacatgg cgaagttttg ctgttgtgtc gcccagcgac atgactaaaa
     2041 tcaggttttc gcaacccact ctgacgccgc gagcccgtcc agctgtcggt tttgtatttt
     2101 ccggccaagg tccccaacat atgcacatgg gtcgtcagct gtacaaagaa ttcccggcat
     2161 tccgcgaaag cgtcacgact ttggacgggg tgtacagcca ggtggtcggc cattctctcg
     2221 tggagaaagt gggcatgttc aaagacatcg gcgccgcatc ctcctccatg cctgacgtct
     2281 ggcctattga tatcattctc cccgcgatcg ccatggttca gatcgcattg ttcgacctcc
     2341 tcgccagcgt cggagtacgg cctgattttg tcttgggcca ttccgccgga gagactacga
     2401 tgttgtacgc gtccggtgct gcccctcggg ctatggctct ggagctcgcg atcgcgcgag
     2461 gacaagtcct ggccctcact gaaagcgccg gcggcaccat gaccgccttg tcctgcgggc
     2521 ctgaagtagc gaagcctcta ctcgacgagg tgaaggcgac gcgtgacgca ggcatcctcg
     2581 aaatcgcatg tcacaacagc gcggaggcca tcacgattgc cggccacgat tatctcgttg
     2641 acgcagctgt ggaactctgc aagtcgaaag gaattgccgc gaccagactg cgtacatccg
     2701 tggccgtgca cagctcaatg atggagcttt gcaaggacga gtactacgaa cgtgtcctgc
     2761 aagtctttgc gaaatatccc gaggcgcgct taacgccaaa tgtggacaca ttctccaccg
     2821 taacgggcga aaggctggcg gagccgttct caccagacta ctactggaaa aacactaggg
     2881 aaccggtgct attcatggag acagttcgct ctgctcaagc cgcgatatcc gggcgtaatc
     2941 tattagtagt cgaagtgagc ccgcatccag ttctctccgc gtacctttcc gcgctttgtc
     3001 cggaatctgc aactatcgtc ggtccgatgc gacgaacgaa gcagatgttc ccacattttg
     3061 agcgttctac cttcttgcaa gcggtcggga agctcatcgc gggcggttac aactcaaccg
     3121 acttccccgc cctcaacggc ggagcgtcgc agcacgtgga gtttggtgtg cctgcatatc
     3181 cctttgcacc gaagagcgtg cctcatatgg cgaaattacc ggagaacgac gagatctatg
     3241 ggtcccgact aggacccttg aaccattctc gacttcgcct caatgcggcg actcatccag
     3301 atctcgcgca acacgtcatg aaaggggaac cgattgttcc tgctacaggt ttcgtggaaa
     3361 tggctctgga gttcggtgcg agatctttat gggacgtgaa gttcctctcc ttcctatctc
     3421 tttcggcgga ggaacctgtc cgcgtccgct tcgagcccga cggcttcaag tggtcgatca
     3481 agacaacttc atatagctca catggttctg gcgcggccag caagaaggcc gagcgattac
     3541 atgctggcgg atacttctcg acagagccaa cagccgccaa ccccccttct cctcgtctag
     3601 acgagattcg ggagaggtgt cattggatca acgtctcagg cttctatgac gacctccgct
     3661 atttcgctga atacggccca tggttccaga tgatcacgtc cgttcatcgc ggcgaggaag
     3721 aatttttggt cgaagttgat acacggcggc agggcgtcaa tgttccgaca tcgcattatg
     3781 tcttcgatcc ggccatcctc gacgcatgca ttcacgttat ggtccatccc atgatcactg
     3841 gcaacactga tcgcaacgta tactacttac catcacgttt cgattttttc tcgacatatg
     3901 acgccttgca tcgtcgcgaa tttacggaca agttgtatgc gcatgggtct cgaggaagat
     3961 ggaccccgga gggcataacc ttcgacgtcc tggtagtcaa tagtcaagga acgccgttgt
     4021 gctccttccg aggctttgag gtcgcctacc acggtttagc gccctcggtg ggaacgcgct
     4081 atgatctcga atacaccact gtggcgagcc tggacctgaa tgcgcgcgtt cagaaggcgg
     4141 actcgttctc gtgcgacgag attactaagg tcacagatcg caccctctac ttccgctaca
     4201 tacgaggcaa ggaggattta ctgcgtagtg agctcgagaa ggtcgacctg actgggttgt
     4261 ccatttggtt gcttgcgctc tccggcgtcg acggcgacgc tgcccttggg ttttcgcgtt
     4321 ccttacgacg cgagctacta gcgaacgagg tgcgcctggt gatattcgag gctcgcatat
     4381 cattcccggc acaagtcgag gtgatatcgg ggttgttaca agagaacgtc gccgaggatg
     4441 aaatcactgt ggactccact ggtcgggttc ttgttcctag gcttcaagcg tcgtcacctc
     4501 cagtagaatc gacttctctg aatcccgacg caccttggtc tgtgaagcgt actggatcgg
     4561 gagattcgaa agcagttatc gttcaaacat cgccgcccat tcccgaacag catgaagtcc
     4621 tggtgaaggt cgattacgtc gatacgctcc agcaatcggg catttgggcc ttcgtagggc
     4681 gtaccgattc cggcactgct gtcgctggac tcgcctctga tcatctggca agccatgttg
     4741 tcgtgcatcg cgcagtcgtc accgagctgt cgaacatcgg gttcgacacc gcggcgcgtc
     4801 ttcctggtta cattgcagct ctctcgatcg gtttcctcgc tttgggattc aaagcggagg
     4861 ttcccagcct cctcgcgcag aacacagcgc tggtcaccca tgccgacacg ctgctaggta
     4921 ccctccttgt ggagcttctc aaggacagag gactgcatgt cacggctgtg aacgaaaccg
     4981 acggccttag taccatctgt ggtctgcaat cgccaaagtc tttcgaccac gttttgagtg
     5041 gattccacga tcccgcctcg ctcagcgtcc ttcacagtat ctgttccaca gaaaatgcag
     5101 tcttcagctg gaaccatccc gatcgcggca ttgctgccat tttaaagaat gatccctaca
     5161 gaatcggtta cgcgcttcgt tccggactta agtctcttcc atcttcatac aagaagatgg
     5221 agtacagcat gcccgttcac cgccttctcc ctttgacctc cgacgtgacc atcctggata
     5281 acactgcgat attcgacccc gcgaagacat atatattgtt cggcggcata ggcagcttgg
     5341 ggttgcgcat tgcgacttgg atgtatgaga acggcgcacg ccatcttgtc ctgacttccc
     5401 gttccggtcg tgaaactctc acaaagacca agaacttcgc cgccatgcgt gttctgcgct
     5461 atttgacgga tttaactgac ctcgacatca agcttctcgc ttccgacgcg acgaacgtca
     5521 cagatctccg cagagtgcta tccgcgataa gttgtcccat cggcggatgc atgatgctaa
     5581 cagctgtcct cgaggacaag atgttcgctt cgttaactca agacagcttt gaaagggtct
     5641 tcgccgcgaa gataggagcc ttcaacgccc tcactcaggc gctcgatatc accagtctcg
     5701 atttcctgat cactttcacg tccatatcag gcatgtttgg cagcgcagga cagaccaact
     5761 atgctgcagc gaacaccgcg ctggaggctt tgactcggcc ttacccaaat gcattctcct
     5821 tggtggcacc cgcgatgctc gacacggcta ttgtcttcgc cgacgagcaa agcaagacta
     5881 ctcataacac caaagtccgc catattagtg aatggggcat gtcgtcgcga gagctttgcg
     5941 catgcatcga agatggcatc cgcaaactgc ggaacgggcc attctggtta tacgttcccg
     6001 atttcaagtg ggcggctgtc cgcgataatc ttggcgtctc tcccgtcttc gcacatctcg
     6061 ttccggaggt tgatgaagac acgtcaacag cggacgcagc ctccaaagac tctagtgtct
     6121 tagatattgt cctcaaggtc ctggacttgg ataaggatga cttttccttt gaggttccac
     6181 tcaccgcata cggactcgac tcgttattgg cgtcgaagct tgcggtaacg cttcgccctt
     6241 gggtttcaat aacccaactg cagctgctgg cagatatcac gttcgaggac atacaacgac
     6301 ggatagaggc gcaggccgga caggaagtcg aacaaccggc cgacaaggac ttctttgact
     6361 gggaggaagt catgcgttcc ggccccgctt gcgtcaagct caccgatggg gaaggcctgc
     6421 cgctgttcgt cctccatggc cctgccggca ctatcgaagc cttcggccac atgcgactca
     6481 atttcaagca tactccgctc tacggagtgc aagccacgaa ggacgcgcct tatgacaacc
     6541 tcgaccgcct tgccgcattc tatcattcga acatcaaaag cgtacaaccg caagggccgt
     6601 accgtttggg cggcttctgc ggctctagtt tactactttt catcctcgcg gatatcttcc
     6661 gcaaaaacgg cgacatcgtc gtgcaaatgt ccatgatcga tcatttccct ctactataca
     6721 cctcgcccat ctgggagctg gacgccgcca cggccaaaga tcgctgcgtt agtgccgccc
     6781 tgaagtcccg cggtttcgag tggatcatga acttgtatcg ggtcgagaaa aatcccaacg
     6841 aaaagcgcgc cggggaggag ctgatgaagg ctgggctggg cgagaaggtt cagccgcacg
     6901 tcaagaagct gtacgaaaac acgatggtgt tcatcgaagc tcacgcatcg tggctcgctt
     6961 cgcacttgct caaggagggc gaggaggttg acggtacgat actgcgtgaa agactcatcg
     7021 cctacctgtc aacattcgac tttcccgcga cggtgtacgc tgccaaagac ggtatgcaga
     7081 agatattcac ggacgagcag caggcgcaat gggcccatat gggcgcaacg atgtgcacca
     7141 atcccattcg tgtcgcggaa atcgaggcgt cgcacttcga tatcctcagc aacctggact
     7201 tcatacatag actcgatacc gaagtaactc tcactgcatc taatgtaatg aaggctatgt
     7261 agggagattg ataagacttt tctagttgca tatcttttat atttaaatct tatctattag
     7321 ttaatttttt gtaatttatc cttatatata gtctggttat tctaaaatat catttcagta
     7381 tctaaaaatt cccctctttt ttcagttata tcttaacagg cgacagtcca aatgttgatt
     7441 tatcccagtc cgattcatca gggttgtgaa gcattttgtc aatggtcgaa atcacatcag
     7501 taatagtgcc tcttacttgc ctcatagaat ttctttctct taacgtcacc gtttggtctt
     7561 ttatagtttc gaaatctatg gtgataccaa atggtgttcc caattcatcg ttacgggcgt
     7621 attttttacc aattgaagta ttggaatcgt caattttaaa gtatatctct cttttacgta
     7681 aagcctgcga gatcctctta agtatagcgg ggaagccatc gttattcgat attgtcgtaa
     7741 caaatacttt gatcggcgct atgcggccgc caccgcggtg gagctccagc ttttgttccc
     7801 tttagtgagg gttaattgcg cgcttggcga gactcaaaca agatatattt tgcccgcctt
     7861 acagacgaaa ctagttggag gttatggagc atactatcac gtgggcggcc actggtgagt
     7921 tactacaccc caggggcaac gttgatgctc ctaaaaaact ctggctggac gcaagccgta
     7981 acacccgtgt cacttcataa tcgtttgcaa ttcagggctt gatctacaca ttaccctgtt
     8041 atccctatta attaatacgg ttatccacag aatcagggga taacgcagga aagaacatgt
     8101 gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc
     8161 ataggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa
     8221 acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc
     8281 ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg
     8341 cgctttctca tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc
     8401 tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc
     8461 gtcttgagtc caacccggta agacacgact tatcgccact ggcagcagcc actggtaaca
     8521 ggattagcag agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg tggcctaact
     8581 acggctacac tagaaggaca gtatttggta tctgcgctct gctgaagcca gttaccttcg
     8641 gaaaaagagt tggtagctct tgatccggca aacaaaccac cgctggtagc ggtggttttt
     8701 ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgatct
     8761 tttctacggg gtctgacgct cagtggaacg aaaactcacg ttaagggatt ttggtcatga
     8821 gattatcaaa aaggatcttc acctagatcc ttttaaatta aaaatgaagt tttaaatcaa
     8881 tctaaagtat atatgagtaa acttggtctg acagttacca atgcttaatc agtgaggcac
     8941 ctatctcagc gatctgtcta tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga
     9001 taactacgat acgggagggc ttaccatctg gccccagtgc tgcaatgata ccgcgagacc
     9061 cacgctcacc ggctccagat ttatcagcaa taaaccagcc agccggaagg gccgagcgca
     9121 gaagtggtcc tgcaacttta tccgcctcca tccagtctat taattgttgc cgggaagcta
     9181 gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt tgccattgct acaggcatcg
     9241 tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc cggttcccaa cgatcaaggc
     9301 gagttacatg atcccccatg ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg
     9361 ttgtcagaag taagttggcc gcagtgttat cactcatggt tatggcagca ctgcataatt
     9421 ctcttactgt catgccatcc gtaagatgct tttctgtgac tggtgagtac tcaaccaagt
     9481 cattctgaga atagtgtatg cggcgaccga gttgctcttg cccggcgtca atacgggata
     9541 ataccgcgcc acatagcaga actttaaaag tgctcatcat tggaaaacgt tcttcggggc
     9601 gaaaactctc aaggatctta ccgctgttga gatccagttc gatgtaaccc actcgtgcac
     9661 ccaactgatc ttcagcatct tttactttca ccagcgtttc tgggtgagca aaaacaggaa
     9721 ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa atgttgaata ctcatactct
     9781 tcctttttca atattattga agcatttatc agggttattg tctcatgagc ggatacatat
     9841 ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg cacatttccc cgaaaagtgc
     9901 cacctgaacg aagcatctgt gcttcatttt gtagaacaaa aatgcaacgc gagagcgcta
     9961 atttttcaaa caaagaatct gagctgcatt tttacagaac agaaatgcaa cgcgaaagcg
    10021 ctattttacc aacgaagaat ctgtgcttca tttttgtaaa acaaaaatgc aacgcgagag
    10081 cgctaatttt tcaaacaaag aatctgagct gcatttttac agaacagaaa tgcaacgcga
    10141 gagcgctatt ttaccaacaa agaatctata cttctttttt gttctacaaa aatgcatccc
    10201 gagagcgcta tttttctaac aaagcatctt agattacttt ttttctcctt tgtgcgctct
    10261 ataatgcagt ctcttgataa ctttttgcac tgtaggtccg ttaaggttag aagaaggcta
    10321 ctttggtgtc tattttctct tccataaaaa aagcctgact ccacttcccg cgtttactga
    10381 ttactagcga agctgcgggt gcattttttc aagataaagg catccccgat tatattctat
    10441 accgatgtgg attgcgcata ctttgtgaac agaaagtgat agcgttgatg attcttcatt
    10501 ggtcagaaaa ttatgaacgg tttcttctat tttgtctcta tatactacgt ataggaaatg
    10561 tttacatttt cgtattgttt tcgattcact ctatgaatag ttcttactac aatttttttg
    10621 tctaaagagt aatactagag ataaacataa aaaatgtaga ggtcgagttt agatgcaagt
    10681 tcaaggagcg aaaggtggat gggtaggtta tatagggata tagcacagag atatatagca
    10741 aagagatact tttgagcaat gtttgtggaa gcggtattcg caatatttta gtagctcgtt
    10801 acagtccggt gcgtttttgg ttttttgaaa gtgcgtcttc agagcgcttt tggttttcaa
    10861 aagcgctctg aagttcctat actttctaga gaataggaac ttcggaatag gaacttcaaa
    10921 gcgtttccga aaacgagcgc ttccgaaaat gcaacgcgag ctgcgcacat acagctcact
    10981 gttcacgtcg cacctatatc tgcgtgttgc ctgtatatat atatacatga gaagaacggc
    11041 atagtgcgtg tttatgctta aatgcgtact tatatgcgtc tatttatgta ggatgaaagg
    11101 tagtctagta cctcctgtga tattatccca ttccatgcgg ggtatcgtat gcttccttca
    11161 gcactaccct ttagctgttc tatatgctgc cactcctcaa ttggattagt ctcatccttc
    11221 aatgctatca tttcctttga tattggatca tactaagaaa ccattattat catgacatta
    11281 acctataaaa ataggcgtat cacgaggccc tttcgtctcg cgcgtttcgg tgatgacggt
    11341 gaaaacctcg gcgcgccatt accctgttat ccctaatata caacgcaaag aagctggaaa
    11401 attggcgagg gaatcctgtt tctgtctatc caagaatggg catgaggtgg caaccgtcgt
    11461 gctagcgtac agggtgcact ttgtaaccat ttgggacacc ggacactcgc tgttttcgaa
    11521 attacccttt aagcgcgggt attgaaccag gcttatgccc aagatcgtag caagcattta
    11581 actgtgggaa tactcaggta tcgtaagatg caagagttcg aatctcttag caaccattat
    11641 ttttttcctc aacataacga gaacacacag gggcgctatc gcacagaatc aaattcgatg
    11701 actggaaatt ttttgttaat ttcagaggtc gcctgacgca tatacctttt tcaactgaaa
    11761 aattgggaga aaaaggaaag gtgagaggcc ggaaccggct tttcatatag aatagagaag
    11821 cgttcatgac taaatgcttg catcacaata cttgaagttg acaatattat ttaaggacct
    11881 attgtttttt ccaataggtg gttagcaatc gtcttacttt ctaacttttc ttacctttta
    11941 catttcagca atatatatat atatttcaag gatataccat tctaatgtct gcccctatgt
    12001 ctgcccctaa gaagatcgtc gttttgccag gtgaccacgt tggtcaagaa atcacagccg
    12061 aagccattaa ggttcttaaa gctatttctg atgttcgttc caatgtcaag ttcgatttcg
    12121 aaaatcattt aattggtggt gctgctatcg atgctacagg tgtcccactt ccagatgagg
    12181 cgctggaagc ctccaagaag gttgatgccg ttttgttagg tgctgtggct ggtcctaaat
    12241 ggggtaccgg tagtgttaga cctgaacaag gtttactaaa aatccgtaaa gaacttcaat
    12301 tgtacgccaa cttaagacca tgtaactttg catccgactc tcttttagac ttatctccaa
    12361 tcaagccaca atttgctaaa ggtactgact tcgttgttgt cagagaatta gtgggaggta
    12421 tttactttgg taagagaaag gaagacgatg gtgatggtgt cgcttgggat agtgaacaat
    12481 acaccgttcc agaagtgcaa agaatcacaa gaatggccgc tttcatggcc ctacaacatg
    12541 agccaccatt gcctatttgg tccttggata aagctaatct tttggcctct tcaagattat
    12601 ggagaaaaac tgtggaggaa accatcaaga acgaattccc tacattgaag gttcaacatc
    12661 aattgattga ttctgccgcc atgatcctag ttaagaaccc aacccaccta aatggtatta
    12721 taatcaccag caacatgttt ggtgatatca tctccgatga agcctccgtt atcccaggtt
    12781 ccttgggttt gttgccatct gcgtccttgg cctctttgcc agacaagaac accgcatttg
    12841 gtttgtacga accatgccac ggttctgctc cagatttgcc aaagaataag gttgacccta
    12901 tcgccactat cttgtctgct gcaatgatgt tgaaattgtc attgaacttg cctgaagaag
    12961 gtaaggccat tgaagatgca gttaaaaagg ttttggatgc aggtatcaga actggtgatt
    13021 taggtggttc caacagtacc accgaagtcg gtgatgctgt cgccgaagaa gttaagaaaa
    13081 tccttgctta aaaagattct ctttttttat gatatttgta cataaacttt ataaatgaaa
    13141 ttcataatag aaacgacacg aaattacaaa atggaatatg ttcatagggt agacgaaact
    13201 atatacgcaa tctacataca tttatcaaga aggagaaaaa ggaggatagt aaaggaatac
    13261 aggtaagcaa attgatacta atggctcaac gaaatgacgg tatcgataag cttgatatcg
    13321 aattcctgca gcccggggga tccactagtt ctagagttaa ttaa
//
