LOCUS       sequence-208196-       12657 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of a fungal biosynthetic gene cluster.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12657)
  AUTHORS   Harvey CJB, Tang M, Schlecht U, Horecka J, Fischer CR, Lin HC, Li J,
            Naughton B, Cherry J, Miranda M, Li YF, Chu AM, Hennessy JR, Vandova
            GA, Inglis D, Aiyar RS, Steinmetz LM, Davis RW, Medema MH, Sattely 
            E, Khosla C, St Onge RP, Tang Y, Hillenmeyer ME
  TITLE     HEx: A heterologous expression platform for the discovery of fungal 
            natural products.
  JOURNAL   Sci Adv. 2018 Apr 11;4(4):eaar5459. doi: 10.1126/sciadv.aar5459. 
            eCollection 2018 Apr.
  PUBMED    29651464
REFERENCE   2  (bases 1 to 12657)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Adv."; 
            date: "2018-04-11"; volume: "4(4):eaar5459. doi"; pages: " 
            10.1126/sciadv.aar5459. eCollection 2018 Apr"
COMMENT     Sequence Label: pCHKU42.2-2.2
FEATURES             Location/Qualifiers
     source          1..12657
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      2034..2333
                     /label=ADH2 terminator
                     /note="transcription terminator for the S. cerevisiae 
                     alcohol dehydrogenase 2 (ADH2) gene"
     promoter        5026..5246
                     /gene="S. cerevisiae URA3"
                     /label=URA3 promoter
     CDS             5247..6050
                     /codon_start=1
                     /gene="S. cerevisiae URA3"
                     /product="orotidine-5'-phosphate decarboxylase, required 
                     for uracil biosynthesis"
                     /label=URA3
                     /note="yeast auxotrophic marker, counterselectable with 
                     5-fluoroorotic acid (5-FOA)"
                     /translation="MSKATYKERAATHPSPVAAKLFNIMHEKQTNLCASLDVRTTKELL
                     ELVEALGPKICLLKTHVDILTDFSMEGTVKPLKALSAKYNFLLFEDRKFADIGNTVKLQ
                     YSAGVYRIAEWADITNAHGVVGPGIVSGLKQAAEEVTKEPRGLLMLAELSCKGSLSTGE
                     YTKGTVDIAKSDKDFVIGFIAQRDMGGRDEGYDWLIMTPGVGLDDKGDALGQQYRTVDD
                     VVSTGSDIIIVGRGLFAKGRDAKVEGERYRKAGWEAYLRRCGQQN"
     primer_bind     complement(8753..8773)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(8753..8771)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     rep_origin      complement(9110..9698)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(9190..9209)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9869..10660,10661..10729))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     10492..10511
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(10730..10834)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      10861..12203
                     /label=2u ori
                     /note="yeast 2u plasmid origin of replication"
     protein_bind    complement(11824..11871)
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     primer_bind     12244..12262
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(12633..12649)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(12633..12649)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
ORIGIN
        1 tatctaaaaa ttgccttatg atccgtctct ccggttacag cctgtgtaac tgattaatcc
       61 tgcctttcta atcaccattc taatgtttta attaagggat tttgtcttca ttaacggctt
      121 tcgctcataa aaatgttatg acgttttgcc cgcaggcggg aaaccatcca cttcacgaga
      181 ctgatctcct ctgccggaac accgggcatc tccaacttat aagttggaga aataagagaa
      241 tttcagattg agagaatgaa aaaaaaaaaa aaaaaaaagg cagaggagag catagaaatg
      301 gggttcactt tttggtaaag ctatagcatg cctatcacat ataaatagag tgccagtagc
      361 gacttttttc acactcgaaa tactcttact actgctctct tgttgttttt atcacttctt
      421 gtttcttctt ggtaaataga atatcaagct acaaaaagca tacaatcaac tatcaactat
      481 taactatatc gtaatacaca atgcagaact ccaccctatt ttcctcagca acacacaacc
      541 tgcctgaggc catgcaggac tacacctggc tggtctttct ccccgctgta gttgttggct
      601 atatcgtcgc cgtcagccgc gaccccttgc aaaaggtccc tggaccgctc cttgctcgtt
      661 ggtcgaacct atggcaggcg ttctacacac gcttcggcat ccgttacaaa gccatccatg
      721 ctgtccataa gacatatgga cctgtcgtga ggatctcgcc gaaccatgtc tcgatcgcag
      781 acatgagtct attaccctct atctacggtc aaggcatggc ggccttcaac aagtcgccgt
      841 tttacgacgc cttcctgtcc gagaagccgt ccatcttcag cactcgggac aagcaagagc
      901 acgcacagaa gcgtcgtaat tattctggtg catttgcgcc taaaacgatt cggtcgtata
      961 cgacaacggt ccatcggttc cttgaggaac tgctggtgaa actcgacaag cgagctgcgc
     1021 tgcctggcga gcctgacaag gccccaatcg acatgctcat ctggtcaaac tacctcgtct
     1081 ttgacatcat gtcgacgctg gcttttggga cgccactcgg catgttggag aaggaatccg
     1141 acgttctcca ggcaggatca ccgaaaggcg ccatagagaa cacggacgtg cgcgagcatt
     1201 acctgaccgt tattggctgg gcgcccgctc ttgcgtacat tgcacgcctc atcccggatc
     1261 cattcttcca gaagggtagc aagagcagcg acgagttgcg cgacatcgcc cggatgtgca
     1321 tcaagcaacg actcgcaagt ggcagcgacg atgccaagag tgacatcctg ggtcatctca
     1381 ttgcagccca catggaatac aaaaaccatc tggacgtgga ggagcttaca tctgaggcgc
     1441 tgactctgct aatcgctggc acagacgcga cttcgaatgc catcacggca atcatccatg
     1501 cgctgtcagt caaccctcga ccacttgcca aactcaggga agagctcgat gaggcgctct
     1561 ctccaggggg cctccagggc cctacatcgg atctgaacga tctaccctac ctgaacgcat
     1621 gcatccatga ggctatccgc ctgcactcac caaccggaat gggcttgcct cggattgtac
     1681 cggagggggg tctcacgtac cgggactact acttcccgcc aggtaccgat gtatctgtgc
     1741 ctacgtggac catgtctcgc gatcgtgcag cctggggaga agatgcagac gtattcagac
     1801 ccgagcgctg gatagaagat ccgtccctca ccaagtactt catggcattc agctcgggta
     1861 ccagaggctg ccttggcaaa agccttgcca tacttgagct gaagatgatc gtggccacgt
     1921 tactccagcg atacgacatc actccccagt cccttgtgct acagaccacg gagaagctta
     1981 tgcataagcc cactcatgac tgggtacgac tgaggcggcg caatgtggat tgagcggatc
     2041 tcttatgtct ttacgattta tagttttcat tatcaagtat gcctatatta gtatatagca
     2101 tctttagatg acagtgttcg aagtttcacg aataaaagat aatattctac tttttgctcc
     2161 caccgcgttt gctagcacga gtgaacacca tccctcgcct gtgagttgta cccattcctc
     2221 taaactgtag acatggtagc ttcagcagtg ttcgttatgt acggcatcct ccaacaaaca
     2281 gtcggttata gtttgtcctg ctcctctgaa tcgtctccct cgatatttct cattttcctt
     2341 cgcatgccag cattgaaatg atcgaagttc aatgatgaaa cggtaattct tctgtcattt
     2401 actcatctca tctcatcaag ttatataatt ctatacggat gtaatttttc acttttcgtc
     2461 ttgacgtcca ccctataatt tcaattattg aaccctcacg atccagttct ccagtgacac
     2521 agcctttatc tggtcaaacc tttctttcta atcacctatg ctgatgctta attaagggat
     2581 ttttgtctcc atcaacggca tgcgcccaaa aatgacgttt tttttaaccc atagacacga
     2641 aactacccat tttccaccgg cctgacctac caccggaaca acggccatct ccaacttgca
     2701 agttggggaa attaagagca tcgcaggttt aatggaagaa aaaaaaaagg tacagcacag
     2761 cgcaaatgga gttagttccc ttatgtcaca cactcacaca cagtcggtca gatcaagcat
     2821 actgggtgcg tataaataga gtggccattg ccaccctgtt tatctcaaaa tctgtcttgt
     2881 tagtggtctt ctcccttttt caggttacaa ttctcttgtt tctacttagt atataagtat
     2941 atcaagctat attaagcata ctatcaactg tcaactctat cctcaaaata caatacaaaa
     3001 tgcgtttgga ggacaacgtg gtggccgtcg caggcgccgc tatggcggtg cacgccatct
     3061 tcaatcgctt cgaatgtgag aaagcatggc cgctgtcggc gttgctgctc ggcgtcccag
     3121 tcaccactgg ccttgcgttg aagtacgctg gtatcgccct cgcatggccc ctactgtttc
     3181 ttgagttcta cggagtcctc ggcgcgtcgg tactacttta tcgtgtctcg ccgttccacc
     3241 ctttggcgcg gtaccccgga ccgattctga acaaactcag caaattctgg atgatgcaca
     3301 tcacgtctca aggcaaacag cacttcttct atcaacatat gcataggcag tttggcactt
     3361 gggtccggac cggccccaac gaattatcca tctgcgacgt cgatgccatc caacctatct
     3421 tgggcaccac cggtttacct aagggtcctc tttgggatgg gcgtgtccct cccggcgcca
     3481 ccactagctt gattggagaa cgcaaccccg ctgaacattc acgtcgccgc aagccctgga
     3541 accgcgggtt cagctcggcg tcgctgaagg gctatgaaga gattcttgaa agccgagctc
     3601 ttcagctcat tgatggtttg cgaaagcaga acaaccctgt caatctcgct cggtggataa
     3661 gctacttctc attcgatctc atgggtgaca tggcgttcgg tggcggcttc gagatgatga
     3721 gggacggtga ccagggcaat attctgcacc tgatcgagaa gggcacccag tctacgtcta
     3781 tgactggtca cgttccgtgg ctgttccgct accttgccaa acttcccaag ttcggaaact
     3841 ccaagttcag agatttcgcg atcaatcgcg ccgacctgcg tcgcaagcaa ggttcgctcc
     3901 ggaaggactt gttccattat atcttcgatg aggctggcgt tgaaccccac ccgccgattc
     3961 tcgaggaggc tctcagtgac ggtattctag ccatagcggc cgggagcgac acgacttccc
     4021 atgtgatgtc gaacatattt tggttcctct tacggcaccc atcatgctac gagcgtctgc
     4081 aagcggaaat cgatgccgcg ttccctcgtg gaacaccggc aacagatgtt gctaaacaaa
     4141 gcgagctgcc atatctcgaa gccgttatca acgaaactct ccgcttgcag ccaccggtac
     4201 ctagtggcgc gcagcgcagt acgcagtaca ctgacggcaa gatggcggga acccactgga
     4261 ttcccaaggg aacgcaggtc caaatcaata cctactcgat ccaacgtgac cctcgctatt
     4321 tctggccagc tcccgaaggc ttctggcccg accgctggct ggaccacgcg cagcgaaaat
     4381 cgaacgaggt cgtggagtac aaggacccca actcgatcat tctcgaaact agagccttca
     4441 tcccgttctc gttcggacct gctatgtgcg tcgcaaagaa attcgccacg atggagatga
     4501 agatggttgt ctgcctcgtt ttgcatacct tccaactccg cttcgccgat ggttggaacg
     4561 cgaatgaatg ggaggagaaa atcggagact acttcacggc tacgcgcggc ccgatgccgg
     4621 tcgttctgac gccaagggct tagacaaatc gctcttaaat atatacctaa agaacattaa
     4681 agctatatta taagcaaaga tacgtaaatt ttgcttatat tattatacac atatcatatt
     4741 tctatatttt taagatttgg ttatataatg tacgtaatgc aaaggaaata aattttatac
     4801 attattgaac agcgtccaag taactacatt atgtgcacta atagtttagc gtcgtgaaga
     4861 ctttattgtg tcgcgaaaag taaaaatttt aaaaattaga gcaccttgaa cttgcgaaaa
     4921 aggttctcat caactgttta aaaggaggat atcaggtcct atttctgaca aacaatatac
     4981 aaatttagtt tcaaagatga atcagtgcgc gaaggacata actcattcaa ttcaattcat
     5041 catttttttt ttattctttt ttttgatttc ggtttctttg aaattttttt gattcggtaa
     5101 tctccgaaca gaaggaagaa cgaaggaagg agcacagact tagattggta tatatacgca
     5161 tatgtagtgt tgaagaaaca tgaaattgcc cagtattctt aacccaactg cacagaacaa
     5221 aaacctgcag gaaacgaaga taaatcatgt cgaaagctac atataaggaa cgtgctgcta
     5281 ctcatcctag tcctgttgct gccaagctat ttaatatcat gcacgaaaag caaacaaact
     5341 tgtgtgcttc attggatgtt cgtaccacca aggaattact ggagttagtt gaagcattag
     5401 gtcccaaaat ttgtttacta aaaacacatg tggatatctt gactgatttt tccatggagg
     5461 gcacagttaa gccgctaaag gcattatccg ccaagtacaa ttttttactc ttcgaagaca
     5521 gaaaatttgc tgacattggt aatacagtca aattgcagta ctctgcgggt gtatacagaa
     5581 tagcagaatg ggcagacatt acgaatgcac acggtgtggt gggcccaggt attgttagcg
     5641 gtttgaagca ggcggcagaa gaagtaacaa aggaacctag aggccttttg atgttagcag
     5701 aattgtcatg caagggctcc ctatctactg gagaatatac taagggtact gttgacattg
     5761 cgaagagcga caaagatttt gttatcggct ttattgctca aagagacatg ggtggaagag
     5821 atgaaggtta cgattggttg attatgacac ccggtgtggg tttagatgac aagggagacg
     5881 cattgggtca acagtataga accgtggatg atgtggtctc tacaggatct gacattatta
     5941 ttgttggaag aggactattt gcaaagggaa gggatgctaa ggtagagggt gaacgttaca
     6001 gaaaagcagg ctgggaagca tatttgagaa gatgcggcca gcaaaactaa aaaactgtat
     6061 tataagtaaa tgcatgtata ctaaactcac aaattagagc ttcaatttaa ttatatcagt
     6121 tattacccta tgcggtgtga aataccgcac agatgcgtaa ggagaaaata ccgcatcagg
     6181 ataggaaaaa accgagcttc ctttcatccg gcgcggctgt gttctacata tcactgaagc
     6241 tccgggtatt ttaagttata caagggaaag atgccggcta gactagcaag ttttaggctg
     6301 cttaacatta tggataggcg gataaagggc ccaaacagga ttgtaaagct tagacgcttc
     6361 tggttggaca atggtacgtt tgtgtattaa gtaaggcttg gctggggata gcaacattgg
     6421 gcagagtata gaagaccaca aaaaaaaggt atataagggc agagaagtct ttgtaatgtg
     6481 tgtaacttct cttccatgtg taatcagtat ttctacttac ttcttaaata tacagaagta
     6541 agacagataa ccaacagcct ttcccagata tacatatata tctttatttc agcttaaaca
     6601 ataattatat ttgtttaact caaaaataaa aaaaaaaaac caaactcacg caactaatta
     6661 ttccataata aaataacaac atggaatccc ttccgatagg agcggtcggc gtcttgctgg
     6721 tctcgattct ccttttcttg gcgcttcgac gttcgaagca gcgtcctccc ctgccaccgg
     6781 ggcccaaggg actccctttg ataggcaatc tcttggatat gccaacgcat tacccatgga
     6841 aaacctatgt agaatggggg cgcagatatt gctcagacgt ggtgcatatt caattgttgg
     6901 gattccatct agttgtcatc aatacagcac gaagtgcggc tgaattgctt gataagaagt
     6961 cgacgatata ctcggatcgc ccatggctga ccatgatgtg cgatctggcg aaccacgcgg
     7021 actgggatat agcgttaatg tccaacaacg ctagatggaa ggcaagccgc gttcagtacc
     7081 agagagttct tagcagcgaa aggtcgaaac aatggaggcg aaagcagacc ttggccgcgt
     7141 acgcattcgg tcgctctctg ctagatgcgc ccggagaact ctttaaacat acacgctact
     7201 ctgtagggtc taatatcctc aatgtgacat acgacataca cgcgttaccc aacggcgatc
     7261 cctggatcaa tctggcagag agagccaacg agatcggaaa cgcggctata agcccgggat
     7321 cctaccttgt ggacgtattt ccaaaactca aataccttcc gtcttggttc cctggcacag
     7381 ggtttaaaag agacgcgctt gaattcaaga aggtcgggga aagcttgctt cacgatccgt
     7441 atgatcacgc gaagaggatg atgttgacgc gcaataacga ggaatctatc gtctccgagc
     7501 ttcttggaga acttgacgat agcgcaactg ctgagcaacg acgggaggtg gagatgacga
     7561 cgaagcggag tacatcggta ctctacgggg ccggcgccga aaccaacgtc gcagccttga
     7621 ataccttctt tctggctatg ctgttgtatc cagacgttca agagaacgcg cggaaggagg
     7681 tcagcagagt caccaaggaa ggccggcttc ctgacttctc ggacgacttg ccatatatac
     7741 gcgccatcgc aaaggagtgt cttagatgga ggccgcccgt cccgctgggc attccacatc
     7801 gggtcaccca agatgacgtt gggctcggtt acagaattcc caagggatct atagtggccg
     7861 gaaatatctg ggccatactg cacgatgagc acgtttaccc ccacacctcg gaattcgacc
     7921 caaaccgctt catgggctcg gaccctaacc ccgacccgga cgccgtgttc ggcttcggca
     7981 gacgtatttg tccgggtcgg ttctttgcca tcgacgcgct ctggatcatg atcgctacca
     8041 ccgtcgcggc gttcgacatt gagaaagcgc tcgacgccga cgggcgtgtc gaagagccca
     8101 cggcacagta tacgtcgggt ttccagagcc gcccacttcc tttcaaatgc acgatcaaac
     8161 cccgtacact ggagatggcg ttattggtcc gcttgaccgc cccggattcc acgtaaggag
     8221 attgataaga cttttctagt tgcatatctt ttatatttaa atcttatcta ttagttaatt
     8281 ttttgtaatt tatccttata tatagtctgg ttattctaaa atatcatttc agtatctaaa
     8341 aattcccctc ttttttcagt tatatcttaa caggcgacag tccaaatgtt gatttatccc
     8401 agtccgattc atcagggttg tgaagcattt tgtcaatggt cgaaatcaca tcagtaatag
     8461 tgcctcttac ttgcctcata gaatttcttt ctcttaacgt caccgtttgg tcttttatag
     8521 tttcgaaatc tatggtgata ccaaatggtg ttcccaattc atcgttacgg gcgtattttt
     8581 taccaattga agtattggaa tcgtcaattt taaagtatat ctctctttta cgtaaagcct
     8641 gcgagatcct cttaagtata gcggggaagc catcgttatt cgatattgtc gtaacaaata
     8701 ctttgatcgg cgctatgcgg ccgccaccgc ggtggagctc cagcttttgt tccctttagt
     8761 gagggttaat tgcgcgcttg gcgagactca aacaagatat attttgcccg ccttacagac
     8821 gaaactagtt ggaggttatg gagcatacta tcacgtgggc ggccactggt gagttactac
     8881 accccagggg caacgttgat gctcctaaaa aactctggct ggacgcaagc cgtaacaccc
     8941 gtgtcacttc ataatcgttt gcaattcagg gcttgatcta cacattaccc tgttatccct
     9001 attaattaat acggttatcc acagaatcag gggataacgc aggaaagaac atgtgagcaa
     9061 aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc
     9121 tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga
     9181 caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc
     9241 cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt
     9301 ctcatagctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct
     9361 gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg
     9421 agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta
     9481 gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct
     9541 acactagaag gacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa
     9601 gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt
     9661 gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta
     9721 cggggtctga cgctcagtgg aacgaaaact cacgttaagg gattttggtc atgagattat
     9781 caaaaaggat cttcacctag atccttttaa attaaaaatg aagttttaaa tcaatctaaa
     9841 gtatatatga gtaaacttgg tctgacagtt accaatgctt aatcagtgag gcacctatct
     9901 cagcgatctg tctatttcgt tcatccatag ttgcctgact ccccgtcgtg tagataacta
     9961 cgatacggga gggcttacca tctggcccca gtgctgcaat gataccgcga gacccacgct
    10021 caccggctcc agatttatca gcaataaacc agccagccgg aagggccgag cgcagaagtg
    10081 gtcctgcaac tttatccgcc tccatccagt ctattaattg ttgccgggaa gctagagtaa
    10141 gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt
    10201 cacgctcgtc gtttggtatg gcttcattca gctccggttc ccaacgatca aggcgagtta
    10261 catgatcccc catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca
    10321 gaagtaagtt ggccgcagtg ttatcactca tggttatggc agcactgcat aattctctta
    10381 ctgtcatgcc atccgtaaga tgcttttctg tgactggtga gtactcaacc aagtcattct
    10441 gagaatagtg tatgcggcga ccgagttgct cttgcccggc gtcaatacgg gataataccg
    10501 cgccacatag cagaacttta aaagtgctca tcattggaaa acgttcttcg gggcgaaaac
    10561 tctcaaggat cttaccgctg ttgagatcca gttcgatgta acccactcgt gcacccaact
    10621 gatcttcagc atcttttact ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa
    10681 atgccgcaaa aaagggaata agggcgacac ggaaatgttg aatactcata ctcttccttt
    10741 ttcaatatta ttgaagcatt tatcagggtt attgtctcat gagcggatac atatttgaat
    10801 gtatttagaa aaataaacaa ataggggttc cgcgcacatt tccccgaaaa gtgccacctg
    10861 aacgaagcat ctgtgcttca ttttgtagaa caaaaatgca acgcgagagc gctaattttt
    10921 caaacaaaga atctgagctg catttttaca gaacagaaat gcaacgcgaa agcgctattt
    10981 taccaacgaa gaatctgtgc ttcatttttg taaaacaaaa atgcaacgcg agagcgctaa
    11041 tttttcaaac aaagaatctg agctgcattt ttacagaaca gaaatgcaac gcgagagcgc
    11101 tattttacca acaaagaatc tatacttctt ttttgttcta caaaaatgca tcccgagagc
    11161 gctatttttc taacaaagca tcttagatta ctttttttct cctttgtgcg ctctataatg
    11221 cagtctcttg ataacttttt gcactgtagg tccgttaagg ttagaagaag gctactttgg
    11281 tgtctatttt ctcttccata aaaaaagcct gactccactt cccgcgttta ctgattacta
    11341 gcgaagctgc gggtgcattt tttcaagata aaggcatccc cgattatatt ctataccgat
    11401 gtggattgcg catactttgt gaacagaaag tgatagcgtt gatgattctt cattggtcag
    11461 aaaattatga acggtttctt ctattttgtc tctatatact acgtatagga aatgtttaca
    11521 ttttcgtatt gttttcgatt cactctatga atagttctta ctacaatttt tttgtctaaa
    11581 gagtaatact agagataaac ataaaaaatg tagaggtcga gtttagatgc aagttcaagg
    11641 agcgaaaggt ggatgggtag gttatatagg gatatagcac agagatatat agcaaagaga
    11701 tacttttgag caatgtttgt ggaagcggta ttcgcaatat tttagtagct cgttacagtc
    11761 cggtgcgttt ttggtttttt gaaagtgcgt cttcagagcg cttttggttt tcaaaagcgc
    11821 tctgaagttc ctatactttc tagagaatag gaacttcgga ataggaactt caaagcgttt
    11881 ccgaaaacga gcgcttccga aaatgcaacg cgagctgcgc acatacagct cactgttcac
    11941 gtcgcaccta tatctgcgtg ttgcctgtat atatatatac atgagaagaa cggcatagtg
    12001 cgtgtttatg cttaaatgcg tacttatatg cgtctattta tgtaggatga aaggtagtct
    12061 agtacctcct gtgatattat cccattccat gcggggtatc gtatgcttcc ttcagcacta
    12121 ccctttagct gttctatatg ctgccactcc tcaattggat tagtctcatc cttcaatgct
    12181 atcatttcct ttgatattgg atcatactaa gaaaccatta ttatcatgac attaacctat
    12241 aaaaataggc gtatcacgag gccctttcgt ctcgcgcgtt tcggtgatga cggtgaaaac
    12301 ctcggcgcgc cattaccctg ttatccctaa tatacaacgc aaagaagctg gaaaattggc
    12361 gagggaatcc tgtttctgtc tatccaagaa tgggcatgag gtggcaaccg tcgtgctagc
    12421 gtacagggtg cactttgtaa ccatttggga caccggacac tcgctgtttt cgaaattacc
    12481 ctttaagcgc gggtattgaa ccaggcttat gcccaagatc gtagcaagca tttaaatgac
    12541 ggtatcgata agcttgatat cgaattcctg cagcccgggg gatttaaatg acggtatcga
    12601 taagcttgat atcgaattcc tgcagcccgg gggatccact agttctagat taattaa
//
