LOCUS       sequence-208197-       14594 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of a fungal biosynthetic gene cluster.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 14594)
  AUTHORS   Harvey CJB, Tang M, Schlecht U, Horecka J, Fischer CR, Lin HC, Li J,
            Naughton B, Cherry J, Miranda M, Li YF, Chu AM, Hennessy JR, Vandova
            GA, Inglis D, Aiyar RS, Steinmetz LM, Davis RW, Medema MH, Sattely 
            E, Khosla C, St Onge RP, Tang Y, Hillenmeyer ME
  TITLE     HEx: A heterologous expression platform for the discovery of fungal 
            natural products.
  JOURNAL   Sci Adv. 2018 Apr 11;4(4):eaar5459. doi: 10.1126/sciadv.aar5459. 
            eCollection 2018 Apr.
  PUBMED    29651464
REFERENCE   2  (bases 1 to 14594)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Adv."; 
            date: "2018-04-11"; volume: "4(4):eaar5459. doi"; pages: " 
            10.1126/sciadv.aar5459. eCollection 2018 Apr"
COMMENT     Sequence Label: pCHKU43.1-2.2
FEATURES             Location/Qualifiers
     source          1..14594
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             504..527
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     primer_bind     complement(9029..9049)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(9029..9047)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     rep_origin      complement(9386..9974)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(9466..9485)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(10145..10936,10937..11005))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     10768..10787
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(11006..11110)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      11137..12479
                     /label=2u ori
                     /note="yeast 2u plasmid origin of replication"
     protein_bind    complement(12100..12147)
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     primer_bind     12520..12538
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        12810..13214
                     /gene="S. cerevisiae LEU2"
                     /label=LEU2 promoter
     CDS             13227..14321
                     /codon_start=1
                     /gene="S. cerevisiae LEU2"
                     /product="3-isopropylmalate dehydrogenase, required for 
                     leucine biosynthesis"
                     /label=LEU2
                     /note="yeast auxotrophic marker"
                     /translation="MSAPKKIVVLPGDHVGQEITAEAIKVLKAISDVRSNVKFDFENHL
                     IGGAAIDATGVPLPDEALEASKKVDAVLLGAVAGPKWGTGSVRPEQGLLKIRKELQLYA
                     NLRPCNFASDSLLDLSPIKPQFAKGTDFVVVRELVGGIYFGKRKEDDGDGVAWDSEQYT
                     VPEVQRITRMAAFMALQHEPPLPIWSLDKANLLASSRLWRKTVEETIKNEFPTLKVQHQ
                     LIDSAAMILVKNPTHLNGIIITSNMFGDIISDEASVIPGSLGLLPSASLASLPDKNTAF
                     GLYEPCHGSAPDLPKNKVDPIATILSAAMMLKLSLNLPEEGKAIEDAVKKVLDAGIRTG
                     DLGGSNSTTEVGDAVAEEVKKILA"
     primer_bind     complement(14569..14585)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(14569..14585)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
ORIGIN
        1 tatctaaaaa ttgccttatg atccgtctct ccggttacag cctgtgtaac tgattaatcc
       61 tgcctttcta atcaccattc taatgtttta attaagggat tttgtcttca ttaacggctt
      121 tcgctcataa aaatgttatg acgttttgcc cgcaggcggg aaaccatcca cttcacgaga
      181 ctgatctcct ctgccggaac accgggcatc tccaacttat aagttggaga aataagagaa
      241 tttcagattg agagaatgaa aaaaaaaaaa aaaaaaaagg cagaggagag catagaaatg
      301 gggttcactt tttggtaaag ctatagcatg cctatcacat ataaatagag tgccagtagc
      361 gacttttttc acactcgaaa tactcttact actgctctct tgttgttttt atcacttctt
      421 gtttcttctt ggtaaataga atatcaagct acaaaaagca tacaatcaac tatcaactat
      481 taactatatc gtaatacaca atggattata aggatgatga tgataagact agtatggcac
      541 acaggcaacc tctagacaat gttgctcggg atgcccccag ctacacaaat ccagaccctc
      601 gtgccgtagc tatcgtcggc atgtctatca gcgcgcccgg cggtgttgac tatggtcttg
      661 ataccgaaga attctatgct ttcctccaaa atagaggatc tgccatcatt accgttccta
      721 ctaaccgctg gaacgcggaa gcctttcatg gcactgggcc gggcaagatc tctactacaa
      781 agggagggtt tattcctgac attagatatg ccgacctcca agagttcggt attactcctg
      841 tggaaggcca acagatatct acttgccaaa tgcttctgct ccatcaagcg ttcacggcgt
      901 tgcagcgcag tggagttgat tatcgtggta caaacactgg ggtttacgtt ggctccgcat
      961 ccggcgaagg aactttgttt gaaatggaca tagcacaggc aggcccgtat tacatgactg
     1021 gaacaaccaa ctcgatcgtt gcgaacagac tgaactacgt atttgatcta ctcggtcctt
     1081 ccatgcccat agatactgcg tgttcgtcct cgctggcagc gatgcacgtc gccgttcaag
     1141 ccgtccgcaa cggtgaatgt gaccaggctg tagtggccgg tgccaacgtc gttcgaaacg
     1201 cactcgaatg cgtagcgttc agtcagttag gtgtgctcag ccctgacggc atatccaagt
     1261 cctttgatga agatgggaat ggatacgcac gtggtgacgt cgccagcgcg gttgttatta
     1321 agcgccatga tctcgccgtc aaagatcaca atcgcattca cgcgacgctt gttggtaccg
     1381 ccctgacatc atgcggatcc atcatgggtt ccctcacaac cccgagtccc gaagcgcagg
     1441 cttcggcgat ccagcttgca tatcgagacg ccggtcttga gcctcatcaa gcagatttcg
     1501 tcgagctcca tggcacgggc acggttgtcg gtgacgcgac agaagcgaat aatgccggcg
     1561 ccgtctttgc gggagggaga gatgcaaggg agatcctcat tggatcggtg aagagcaacg
     1621 ttggacatgg tgaactaggc gcgtatatga catcattagt gaaggtcgca atgatgttgg
     1681 aaaggaagga agtcctacct aatggctact tcaaaaagcc atcctcccga attgatttcg
     1741 aaaagttcaa gctgcgtgtt cccgtgtccg ttgagaattt cgcacctggc aatccccaac
     1801 tcggtcggat tgcgtctata tctagttacg gatttggagg cgctctgtcg ctgaagggat
     1861 gcgttactct cagcgagaaa tacaaagaag agtgcgctga agttgagcct ctcgccctgt
     1921 gcgagcactt ggcaaaccgt acacgccaac tcccatggag gtccttcgca atcgctggat
     1981 ccatcgagac agcgacgttc cccgaaccag tcctcgtacc taaacgcccc catcctctcg
     2041 tcttttgttt ctcaggccag ggcccccaac actggcagca aggccgggag ctatatcata
     2101 ctttcaaggt gttccgcgat agtatcaatg actgcgacag ggtgcatgtc gattacacag
     2161 gggaatcctt catcgagacg acaggcctgt tcaagaccga tgctcctaag gactcggacc
     2221 tggctaccag cttaacatgg ccttccgatg tcacttccat ttctctcacc ttcttccaga
     2281 ttgcgctctt cgacctcttg agttctctga atcttaagcc agatacaatt gttggacact
     2341 cagttggaga gacagcagtc ttatacgctt caggcgcggt cccacgtagc atggccgtta
     2401 aaatcgcgat cgctcgtgga aaagctcttg ccatggttga caacactggc ggcggtatgg
     2461 tggctgtttc tggatgcgac gcagacaaag tacgcgacta cgcggaaacg gcaattacac
     2521 tagctgtgca ggaagtcgac tcggcgggtg gtccccagct gttccttgcg tcattcaaca
     2581 gccccacaga tataggcgta tcaggctcca agaaacatct tgaggtattt gcaagcttca
     2641 tcgaaagatg ggtgagcgat gtcgtcgcgc gcaaacttcg catcaacacc gctgtccact
     2701 ctcccttcgt ccatccctgc gagtcgcaat accgccgcga cctagctacc attttcgcgg
     2761 agtatccagg tcctcacatc ccaaagatcc cgacgatgtc cacggtcact gctgagttca
     2821 aaacagactc gtatacgccc gattatttgt ggaacaacct ccgccaacct gtgcttttct
     2881 cttcggctat ccccaaagta gttgagagac tcggagaatt gaccacgttt attgaggtcg
     2941 cccctcatcc agtcctgtcc cagtacctca agcagatggg tgcccttgat gctctcgcct
     3001 gcagcagacg tccaccgtct gcccgccatg taaaacctgg gtcgagcgct ttggtcgaag
     3061 tacgcacatt gctggaagtt ttgggacgtt tgctgttagt cggcgtcaat aagatcaact
     3121 tctcggagtt tgccggatgc ccagatgcaa acatcgaggg tcccgcatat ccgttccagc
     3181 caaagttatg gcgctatggc atggatgaac catcatacgt ccgtaagcta ttacccgcgg
     3241 aaaggtcttt gaattcgatt cggctacgcg tcagcccaat tatgcccgag ccgtggatgg
     3301 gtgatcatgt cattgatcat acgaatctgg ttcccgcggc tgcctaccta gagatggccc
     3361 ttgagttccc cgatgttact cagctgtatg attgccgctt cgaggctatg tatgtcctgg
     3421 aagagtcagg accacctggg acattggaag tttcccggga agggaattcc tggtgggtga
     3481 agtcatcatc tcagatgcgc actccgcagg gggacaagga gtggacgagg actggtcctt
     3541 ctttcgacac tctccactct tgcggcaagt tgggttatgg tccccctgaa atcgagccgg
     3601 atgcgatcac acgcgtcgat cttgaggaag tgctgtctcg ctgcccctac acgttcgatc
     3661 gcgacgcctt taacgatcgg atggagggtg tggtacaatt tggtcccgag ttcagacgaa
     3721 ttatgaaagc ggtgtgcaat gaaaaggaag tcctgacata tatcaagggt catgtggatg
     3781 gcctgaatca gacagattat gccttccatc ccgccctctt agatgccgtc ttccagtcgc
     3841 ctctttcatg gggcctgatc ggtgataagc tgcatctcgg taggaagaat acccagctac
     3901 tgctgccaca ttctgttcgc cgcatattcc gcaatgatgg cagtactgct ccactggtgc
     3961 tgcctgatga atttgtagct tataccgtcc tccacgagtg gtcgcctcac cactgggtga
     4021 cgaactgtta tatcttaggt gaagaccagt cggtcatata taccgtggaa ggacttcgtt
     4081 ttgcgttggt ccagcatgac aatgagtggc ctagcgaaca cttcaagatg ttctggcaac
     4141 ctagggactt gccacagtcg catctggaaa gctcagtcat tcttcctggt ccaccaccgc
     4201 attccgatga tatagagtta tttggtctac ctcacgactt cgctcctacc tcgcctgacc
     4261 gccagcgcta cttggcttgg gcaaatgcgg tggccaccag actgaccgcg aacaacgctg
     4321 atgtgccagc agtccctgct catttgcaag aaaagtataa agctttgttc gacctgactc
     4381 agagggtcgg tgctggacaa aaagctgccg ccgttgagct gcttttctat gacgacatca
     4441 tgagcaagat ctacgagcat ccacctttca tcggctctgt attcgatgcg accacgtcgc
     4501 gtttcatcga ccttgttacc gacgcactca atgctggcaa acaggtggtc cgagtccttg
     4561 aggttggtgc gggaactggg aggttcactg cactgctcgg gcgagctctt ttggaagctg
     4621 ctttgcctga aggctgctac gtcgactacg tctgcaccga cgtcagtatt tcgctcgcac
     4681 aagaggcgac agcgaagtcc ccttggccaa cggtaaccgc caaagccctt gatctgaatg
     4741 tcgcgatcag cgaacagaac ctagatcccc gcagtttcga tattgtcgtt gcctttgatg
     4801 ttctgcacgc tatccctgac attcacgcga gtttgacgac gctccagcag ctcctctgtc
     4861 ccggcggtca cattgccatc ttggagttgg acggtggttc caaacctgta ctgctccgac
     4921 acttcaccgc tggaggggaa gttgcccttg tcgacttcat atcatcgctg gattcaacat
     4981 acccctacct cttctggcta tacaccacta cttcccccgg cgatgcagct gcggttggac
     5041 tagtgagaac tctccgccag gaatacccaa cctggaagat tcacctcgct ctcttccccg
     5101 actcctggac ggtgacagcc caagaggctt atgtgcactc acagctcata ctgttgccgt
     5161 gggtggactc tgaagtgatg attgaccagg acgggaagat gcacgtgccg cgtctggtga
     5221 aggcctctcc cgccgctcgc caggaacttc ggggctctca tccagtctca ttccggggtt
     5281 ccgatgtctg gcgagcgtac ccagcggagc ttggtccgaa tgatgttgaa gtgtccgttc
     5341 actatgttgg attgactccg gctatgtctg gatggaccga atttgctgga gcggtgacag
     5401 ccgtcggctc tggtgtactt gaagaggacc tcgtcgggcg cagggtattt ggtgttactg
     5461 tttctgtccc cggaagtacc atcgtctgcg atcgttccaa gcttgcgttc gttcctcaag
     5521 acatggaaac tgccctggcc accgctttgg tgggaaggct tatgttctta tcattggtcg
     5581 tggtcgatac cgtcggcacc tcttcgaaga acacaaaggt cctcctccac gctggccatt
     5641 gttcaccggc cgcgctcgcg acatacgctt acctaagtgc caactccttc aacgttactg
     5701 tcaccgtcag cgatgcacca ccgtccacca acaccttcag cacgctgtcc ccgatttcat
     5761 cttccaaata ctatgcctgg tcatctcgtg tccgcgcttg ggctccacga ggcgtagacg
     5821 tcgtcttcaa ttttgacgag gatatcaacg tggcgaagca aacgctttcc ttgctttctt
     5881 ctcgggggag atttgttcaa atagggggag agctgcccag ctctcttcct tccggacgtc
     5941 gctacgtatc aatcgaagag catgctgttc tagaagctgg caatgatctc gatcgcaccg
     6001 acgctgtttt ttcacccgct gtccttgctg ccctacctcc tgccgtggac atttacaacc
     6061 tcccacagct catgaccgcc gacgtccaag cacgcaactc gatgccaggc cgtgttactc
     6121 tggtcgacct gcaagcgatt ccccaggagc ttcccgtttt gaaagccgga atgttgaaag
     6181 ggacggctgt tttcaatcct cgagcatcct atgtgattgt gggtggtatt ggcggactgg
     6241 gtgtcaacat tgcacggtgc ttggtggaaa atggtgccaa gcacctgatt ctgacttcga
     6301 gatctgggga gaagggaatc gcgactatgc aactagcacg cgagaaaaag attgtcaatt
     6361 atcttcgagg aattcctgga gtaaccgtcg atcttcgggc ggtggacgtt cttgacgctg
     6421 agaagaccag agacttgttc atgaacctgg agcatcctgt tgccggggtg ttctatttgg
     6481 ctgtcaggct caacgaccag gtctttgcga atttgaagac ggaggaagac tggaaaattg
     6541 gcaagtcggc tatcttgcgc tttatttacg acgtcaagat caaaggcgtt gaagttctcc
     6601 tgcaagcggt caaccctcag tcactggatt tccttgtcct cacgagttcc atggctactg
     6661 tgtgcggctc gccaggccag gcgaactatt ctgctgccca gacctacatg gagactatgg
     6721 cggcagcgct tcccaatacc gttgcaatca ccgtgccccc tatcaccgat ggaggtgtgt
     6781 ttgtccgaag cctgcctcca ggtaacgcac gcaatgcagc cctggacaaa tacaaatccc
     6841 tcggaatgac tggctacctt gttgcgcaac actgcgtgaa tgccatctgg acgctgaact
     6901 gcgatgccta tagcccagtc tatattcctc ccaccgactg gaaagagatc atgcaacttg
     6961 gcatcccgga ataccacttg tcgtccctgc gtcatttgtt gaccaagcaa aacgttgaga
     7021 tcgcgctggc aaccgaggag tggtccattc gggcggcgtg cgcatcggtg ttggctcttg
     7081 ataccaacga ggttgcagac aatgtcccgc tttcaagcta tggtcttgac tcgctaacat
     7141 ccgttcgatt gagtgggatt ctcaaaacca acttcaatgt ttccgtgact cagcttcagc
     7201 ttctcggaaa cagtatgact gtccaacgac tgatggctct gcaagaggag ctgaaggccg
     7261 attcatcttc tgtggctgtg gggactgcca acaaggactc gctcgacata ctgggagccg
     7321 aaggtgtcca tcatgctgac atggacaaga cgatcgttcg attgaacaac gtcacggatg
     7381 gacaacctgt attcatcgta cacggcgctg gcggcggcgt cctcgtcatg ctgaaaaccg
     7441 cggaaatgat gtcttgtcct gtttatggtg tccaagacac tccagatgcg cccattaccg
     7501 gaaccctcga caaactttgc aggttctacc tggagaaaat ccgagagaag caacccaaag
     7561 gtccataccg ccttgggggt ttctcattcg gcacctatgc cgcttttgtc atcgcacagc
     7621 tgctgcaaga agaaggggag acagttgagc tgctcgtcat gatcgatggg tcgccagcga
     7681 ttttcaccaa cttcgccaag aggatcaatg tgaccaatat gcgagacgaa atcatgaccg
     7741 ttatccgtga tcttgccgca agcgggacgc tggaaagtgg cgaggaactt accgccatgt
     7801 ttgaggacca cttcaagcaa gttgcgcagg gcggttctag cggcaaattt gtgtcccagt
     7861 tcggtcgtgc attcgtcgca cacgtgctca tggcggtccg cgcttgtatc ccagcgatct
     7921 tacgggagca agctggggag atcgataact ctgtctggcc gaccgcgaga acggtcgtta
     7981 tccgtgcgga agatggcatt ggtcgtcaat ccgtgtcgga aggactttcc cctgcttttg
     8041 atgtagaatt acgcgcccca gatgcggagt tttacatcct accgggaacg cacttcggga
     8101 ttctccaccc ccagtctggg ctccctcagc ttctcgaaca ggtggagaag tgtgatcacc
     8161 caaaagtgat accaccgacg atgacgatga tgaccggaac tacgatttct gtgggaaaac
     8221 acagaagtca gtcatccatc gacttaggct gctctgacac ggcgagggca caagctgcga
     8281 aagaagtttc cgggatcatg gtgtcatcaa tgtggaggag ctggacagcg aagagcacca
     8341 agggtgtgac agcttcggca ttctcagaca gcatcagggt ttccgcctac aatgcgtccc
     8401 ccagtaggct cctgctgcct tcattggggg ctaatgcagg ccctcaatgc gacggcggac
     8461 gaaaatggta ccaggcccca aaaatgacct agggagattg ataagacttt tctagttgca
     8521 tatcttttat atttaaatct tatctattag ttaatttttt gtaatttatc cttatatata
     8581 gtctggttat tctaaaatat catttcagta tctaaaaatt cccctctttt ttcagttata
     8641 tcttaacagg cgacagtcca aatgttgatt tatcccagtc cgattcatca gggttgtgaa
     8701 gcattttgtc aatggtcgaa atcacatcag taatagtgcc tcttacttgc ctcatagaat
     8761 ttctttctct taacgtcacc gtttggtctt ttatagtttc gaaatctatg gtgataccaa
     8821 atggtgttcc caattcatcg ttacgggcgt attttttacc aattgaagta ttggaatcgt
     8881 caattttaaa gtatatctct cttttacgta aagcctgcga gatcctctta agtatagcgg
     8941 ggaagccatc gttattcgat attgtcgtaa caaatacttt gatcggcgct atgcggccgc
     9001 caccgcggtg gagctccagc ttttgttccc tttagtgagg gttaattgcg cgcttggcga
     9061 gactcaaaca agatatattt tgcccgcctt acagacgaaa ctagttggag gttatggagc
     9121 atactatcac gtgggcggcc actggtgagt tactacaccc caggggcaac gttgatgctc
     9181 ctaaaaaact ctggctggac gcaagccgta acacccgtgt cacttcataa tcgtttgcaa
     9241 ttcagggctt gatctacaca ttaccctgtt atccctatta attaatacgg ttatccacag
     9301 aatcagggga taacgcagga aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc
     9361 gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca
     9421 aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt
     9481 ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc
     9541 tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc
     9601 tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc
     9661 ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact
     9721 tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg
     9781 ctacagagtt cttgaagtgg tggcctaact acggctacac tagaaggaca gtatttggta
     9841 tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca
     9901 aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa
     9961 aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg
    10021 aaaactcacg ttaagggatt ttggtcatga gattatcaaa aaggatcttc acctagatcc
    10081 ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa acttggtctg
    10141 acagttacca atgcttaatc agtgaggcac ctatctcagc gatctgtcta tttcgttcat
    10201 ccatagttgc ctgactcccc gtcgtgtaga taactacgat acgggagggc ttaccatctg
    10261 gccccagtgc tgcaatgata ccgcgagacc cacgctcacc ggctccagat ttatcagcaa
    10321 taaaccagcc agccggaagg gccgagcgca gaagtggtcc tgcaacttta tccgcctcca
    10381 tccagtctat taattgttgc cgggaagcta gagtaagtag ttcgccagtt aatagtttgc
    10441 gcaacgttgt tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt
    10501 cattcagctc cggttcccaa cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa
    10561 aagcggttag ctccttcggt cctccgatcg ttgtcagaag taagttggcc gcagtgttat
    10621 cactcatggt tatggcagca ctgcataatt ctcttactgt catgccatcc gtaagatgct
    10681 tttctgtgac tggtgagtac tcaaccaagt cattctgaga atagtgtatg cggcgaccga
    10741 gttgctcttg cccggcgtca atacgggata ataccgcgcc acatagcaga actttaaaag
    10801 tgctcatcat tggaaaacgt tcttcggggc gaaaactctc aaggatctta ccgctgttga
    10861 gatccagttc gatgtaaccc actcgtgcac ccaactgatc ttcagcatct tttactttca
    10921 ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg
    10981 cgacacggaa atgttgaata ctcatactct tcctttttca atattattga agcatttatc
    11041 agggttattg tctcatgagc ggatacatat ttgaatgtat ttagaaaaat aaacaaatag
    11101 gggttccgcg cacatttccc cgaaaagtgc cacctgaacg aagcatctgt gcttcatttt
    11161 gtagaacaaa aatgcaacgc gagagcgcta atttttcaaa caaagaatct gagctgcatt
    11221 tttacagaac agaaatgcaa cgcgaaagcg ctattttacc aacgaagaat ctgtgcttca
    11281 tttttgtaaa acaaaaatgc aacgcgagag cgctaatttt tcaaacaaag aatctgagct
    11341 gcatttttac agaacagaaa tgcaacgcga gagcgctatt ttaccaacaa agaatctata
    11401 cttctttttt gttctacaaa aatgcatccc gagagcgcta tttttctaac aaagcatctt
    11461 agattacttt ttttctcctt tgtgcgctct ataatgcagt ctcttgataa ctttttgcac
    11521 tgtaggtccg ttaaggttag aagaaggcta ctttggtgtc tattttctct tccataaaaa
    11581 aagcctgact ccacttcccg cgtttactga ttactagcga agctgcgggt gcattttttc
    11641 aagataaagg catccccgat tatattctat accgatgtgg attgcgcata ctttgtgaac
    11701 agaaagtgat agcgttgatg attcttcatt ggtcagaaaa ttatgaacgg tttcttctat
    11761 tttgtctcta tatactacgt ataggaaatg tttacatttt cgtattgttt tcgattcact
    11821 ctatgaatag ttcttactac aatttttttg tctaaagagt aatactagag ataaacataa
    11881 aaaatgtaga ggtcgagttt agatgcaagt tcaaggagcg aaaggtggat gggtaggtta
    11941 tatagggata tagcacagag atatatagca aagagatact tttgagcaat gtttgtggaa
    12001 gcggtattcg caatatttta gtagctcgtt acagtccggt gcgtttttgg ttttttgaaa
    12061 gtgcgtcttc agagcgcttt tggttttcaa aagcgctctg aagttcctat actttctaga
    12121 gaataggaac ttcggaatag gaacttcaaa gcgtttccga aaacgagcgc ttccgaaaat
    12181 gcaacgcgag ctgcgcacat acagctcact gttcacgtcg cacctatatc tgcgtgttgc
    12241 ctgtatatat atatacatga gaagaacggc atagtgcgtg tttatgctta aatgcgtact
    12301 tatatgcgtc tatttatgta ggatgaaagg tagtctagta cctcctgtga tattatccca
    12361 ttccatgcgg ggtatcgtat gcttccttca gcactaccct ttagctgttc tatatgctgc
    12421 cactcctcaa ttggattagt ctcatccttc aatgctatca tttcctttga tattggatca
    12481 tactaagaaa ccattattat catgacatta acctataaaa ataggcgtat cacgaggccc
    12541 tttcgtctcg cgcgtttcgg tgatgacggt gaaaacctcg gcgcgccatt accctgttat
    12601 ccctaatata caacgcaaag aagctggaaa attggcgagg gaatcctgtt tctgtctatc
    12661 caagaatggg catgaggtgg caaccgtcgt gctagcgtac agggtgcact ttgtaaccat
    12721 ttgggacacc ggacactcgc tgttttcgaa attacccttt aagcgcgggt attgaaccag
    12781 gcttatgccc aagatcgtag caagcattta actgtgggaa tactcaggta tcgtaagatg
    12841 caagagttcg aatctcttag caaccattat ttttttcctc aacataacga gaacacacag
    12901 gggcgctatc gcacagaatc aaattcgatg actggaaatt ttttgttaat ttcagaggtc
    12961 gcctgacgca tatacctttt tcaactgaaa aattgggaga aaaaggaaag gtgagaggcc
    13021 ggaaccggct tttcatatag aatagagaag cgttcatgac taaatgcttg catcacaata
    13081 cttgaagttg acaatattat ttaaggacct attgtttttt ccaataggtg gttagcaatc
    13141 gtcttacttt ctaacttttc ttacctttta catttcagca atatatatat atatttcaag
    13201 gatataccat tctaatgtct gcccctatgt ctgcccctaa gaagatcgtc gttttgccag
    13261 gtgaccacgt tggtcaagaa atcacagccg aagccattaa ggttcttaaa gctatttctg
    13321 atgttcgttc caatgtcaag ttcgatttcg aaaatcattt aattggtggt gctgctatcg
    13381 atgctacagg tgtcccactt ccagatgagg cgctggaagc ctccaagaag gttgatgccg
    13441 ttttgttagg tgctgtggct ggtcctaaat ggggtaccgg tagtgttaga cctgaacaag
    13501 gtttactaaa aatccgtaaa gaacttcaat tgtacgccaa cttaagacca tgtaactttg
    13561 catccgactc tcttttagac ttatctccaa tcaagccaca atttgctaaa ggtactgact
    13621 tcgttgttgt cagagaatta gtgggaggta tttactttgg taagagaaag gaagacgatg
    13681 gtgatggtgt cgcttgggat agtgaacaat acaccgttcc agaagtgcaa agaatcacaa
    13741 gaatggccgc tttcatggcc ctacaacatg agccaccatt gcctatttgg tccttggata
    13801 aagctaatct tttggcctct tcaagattat ggagaaaaac tgtggaggaa accatcaaga
    13861 acgaattccc tacattgaag gttcaacatc aattgattga ttctgccgcc atgatcctag
    13921 ttaagaaccc aacccaccta aatggtatta taatcaccag caacatgttt ggtgatatca
    13981 tctccgatga agcctccgtt atcccaggtt ccttgggttt gttgccatct gcgtccttgg
    14041 cctctttgcc agacaagaac accgcatttg gtttgtacga accatgccac ggttctgctc
    14101 cagatttgcc aaagaataag gttgacccta tcgccactat cttgtctgct gcaatgatgt
    14161 tgaaattgtc attgaacttg cctgaagaag gtaaggccat tgaagatgca gttaaaaagg
    14221 ttttggatgc aggtatcaga actggtgatt taggtggttc caacagtacc accgaagtcg
    14281 gtgatgctgt cgccgaagaa gttaagaaaa tccttgctta aaaagattct ctttttttat
    14341 gatatttgta cataaacttt ataaatgaaa ttcataatag aaacgacacg aaattacaaa
    14401 atggaatatg ttcatagggt agacgaaact atatacgcaa tctacataca tttatcaaga
    14461 aggagaaaaa ggaggatagt aaaggaatac aggtaagcaa attgatacta atggctcaac
    14521 gaaatgacgg tatcgataag cttgatatcg aattcctgca gcccggggga tccactagtt
    14581 ctagagttaa ttaa
//
