LOCUS       sequence-208199-       13574 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of a fungal biosynthetic gene cluster.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13574)
  AUTHORS   Harvey CJB, Tang M, Schlecht U, Horecka J, Fischer CR, Lin HC, Li J,
            Naughton B, Cherry J, Miranda M, Li YF, Chu AM, Hennessy JR, Vandova
            GA, Inglis D, Aiyar RS, Steinmetz LM, Davis RW, Medema MH, Sattely 
            E, Khosla C, St Onge RP, Tang Y, Hillenmeyer ME
  TITLE     HEx: A heterologous expression platform for the discovery of fungal 
            natural products.
  JOURNAL   Sci Adv. 2018 Apr 11;4(4):eaar5459. doi: 10.1126/sciadv.aar5459. 
            eCollection 2018 Apr.
  PUBMED    29651464
REFERENCE   2  (bases 1 to 13574)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Adv."; 
            date: "2018-04-11"; volume: "4(4):eaar5459. doi"; pages: " 
            10.1126/sciadv.aar5459. eCollection 2018 Apr"
COMMENT     Sequence Label: pCHSU62.1-2.2
FEATURES             Location/Qualifiers
     source          1..13574
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             504..527
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             complement(6424..6435)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(8009..8029)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(8009..8027)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     rep_origin      complement(8366..8954)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8446..8465)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9125..9916,9917..9985))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9748..9767
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9986..10090)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      10117..11459
                     /label=2u ori
                     /note="yeast 2u plasmid origin of replication"
     protein_bind    complement(11080..11127)
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     primer_bind     11500..11518
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        11790..12194
                     /gene="S. cerevisiae LEU2"
                     /label=LEU2 promoter
     CDS             12207..13301
                     /codon_start=1
                     /gene="S. cerevisiae LEU2"
                     /product="3-isopropylmalate dehydrogenase, required for 
                     leucine biosynthesis"
                     /label=LEU2
                     /note="yeast auxotrophic marker"
                     /translation="MSAPKKIVVLPGDHVGQEITAEAIKVLKAISDVRSNVKFDFENHL
                     IGGAAIDATGVPLPDEALEASKKVDAVLLGAVAGPKWGTGSVRPEQGLLKIRKELQLYA
                     NLRPCNFASDSLLDLSPIKPQFAKGTDFVVVRELVGGIYFGKRKEDDGDGVAWDSEQYT
                     VPEVQRITRMAAFMALQHEPPLPIWSLDKANLLASSRLWRKTVEETIKNEFPTLKVQHQ
                     LIDSAAMILVKNPTHLNGIIITSNMFGDIISDEASVIPGSLGLLPSASLASLPDKNTAF
                     GLYEPCHGSAPDLPKNKVDPIATILSAAMMLKLSLNLPEEGKAIEDAVKKVLDAGIRTG
                     DLGGSNSTTEVGDAVAEEVKKILA"
     primer_bind     complement(13549..13565)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(13549..13565)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
ORIGIN
        1 tatctaaaaa ttgccttatg atccgtctct ccggttacag cctgtgtaac tgattaatcc
       61 tgcctttcta atcaccattc taatgtttta attaagggat tttgtcttca ttaacggctt
      121 tcgctcataa aaatgttatg acgttttgcc cgcaggcggg aaaccatcca cttcacgaga
      181 ctgatctcct ctgccggaac accgggcatc tccaacttat aagttggaga aataagagaa
      241 tttcagattg agagaatgaa aaaaaaaaaa aaaaaaaagg cagaggagag catagaaatg
      301 gggttcactt tttggtaaag ctatagcatg cctatcacat ataaatagag tgccagtagc
      361 gacttttttc acactcgaaa tactcttact actgctctct tgttgttttt atcacttctt
      421 gtttcttctt ggtaaataga atatcaagct acaaaaagca tacaatcaac tatcaactat
      481 taactatatc gtaatacaca atggattata aggatgatga tgataagact agtatggaga
      541 agccgatgcc tattgccatc attggcatgg catgccgcac ttcgggcggc gttgcttcat
      601 tagatgaatt ctggacgatg ctctctcgct gcagagacgg ctctggtccg attccaaagg
      661 acagattctc ctctgctgca tactatcacc ccaatccgca aaagcgaggc acattcaacc
      721 agcttggagg ctatttcatg gacagagatg tgtcctactt cgacgccccg ttctttaata
      781 tcacaaggca agaagcagct tcactagatc cgctgcaaag acaactcctc gaaatctcat
      841 acgaagctct agaatgcgcc gggcttccta agaacaagat atcgggagag agcatggccg
      901 tcttcgttgg tactaagaga tcagactacc gatgcaactc acaggacgtg aagcgaattg
      961 aactgtttaa cgcaacaagc gttcacccgg ccgttcaggc aggccggcta tcctactact
     1021 tcaacctcaa cgggccctgc tgcgccgtcg acacagcctg ctcttctggg ctgcatgctc
     1081 tccacctagg cgtgaatgcc attcgtaatg gagagtgcag ttctgcgctt gtggcggctg
     1141 ctaatttgta tctcaacccg gatgacctgg tatccatgtc gatgctgggc ctatttactc
     1201 ctgcgggcaa gacatacgcc tttgaccacc gcggaacgtc cggctttgcc tgcggcgaag
     1261 gcgcggcagc cctcgtcatc aagccgctgg atcgggccat taatgataac gacaacatct
     1321 actctgtaat cgtcaacaca ggaatcaacc aggatggcaa gacggctggc ctcacaacgc
     1381 cgagcagtga aatgcaagag aagcttatgc gtgacgtata caggcgcgca aacatcagcc
     1441 ctaatgacac agcattcgtc gaggctcacg gcacaggcac aaaagtagga gatcccctag
     1501 aagttgctgc catccagcga gtgtttggca acgggcgtac aaagcgcagc ccgctatata
     1561 ttggctcagt aaagaccaat gtcggccacc tggaaaatgc cagcgggctc atttccgtca
     1621 tcaaggctag tcttatgctg caccgaggct tcatcttgcc aaacaccaat tttgagaagg
     1681 cgaatcctga tatcccactg gaagcgtgga atttgaaggt ccccgtcaat attcgtccct
     1741 ggcccagggg caggcgctat atcagcatca acaactttgg tttcggcggc tctaacgccc
     1801 atgtcgtcct tgataaagcg cctcccgcgg ttatgctcag agctattcct gtcgagccgc
     1861 cgggcagaga agctatacct aaactctttg tcatttccgg tcacgacgag ggtgcagcaa
     1921 gaagaaatgc cagccaaatt ggaatctacg tcgagcagca cccggaagtc tttgagaagc
     1981 gcatcgctcg cgacgtcgcg tacaccctcg gcgagcgtcg aactcaccat gctttccgcg
     2041 ttgctctcac agcgagcaat ttcggtgaac tgacagaaga tttgaacgat gaggcactac
     2101 tcccacagcg tgcgtcagat gaaccactta agctagcctt catattcact ggccaaggtg
     2161 ctcagtggcc gcagatgggc agacagttac tcaattctca cccgatcttc ggcctgacgg
     2221 tcaaggagtg ctccgatttt ctagagaaca gtattggcgc tgacttctct cttatgcaag
     2281 aactcttgcg gccaaagggc gagtccatcg ttaatgaagc tcacatttca cagccaattt
     2341 gcacagcaat ccagctgggt cttgttgagc tgctcaagtc ctggaaaatt gagccctcca
     2401 tggtttgtgg ccattcgtct ggtgagatag ccgccgccta tgctgccggt gccattacgc
     2461 tcagagatgc tatggccgct gcttattacc gcggtcactt tgccgctcag atgaagaaac
     2521 gcaatcctaa agtccgtggt gccatgttag ctgttggcgc cggacctgcg gaagtaaaga
     2581 gaatcatcaa gtctctgagt ttagccggcg tcaccgtttc ttgtgaaaac gctcccaatt
     2641 ctgtaactgc ttctggtgac gaagccgaca ttgacagact tgcattagaa cttgaaggac
     2701 gcagcttgtt caaccgtaag ctccgtgtcg atgttgcata tcactctgcg catatgaagc
     2761 tcgttgcgaa cgactacatg gcagccattg agaacatgca cttcaaggcc atggatgatg
     2821 gtatcaagtt ttactcgtct cttcttggga aaaagcttga tagcactgaa gcactgggtg
     2881 ctcagtactg ggttaacaac cttatcaagc ctgttctctt ttcatctgct cttgaggagc
     2941 tctacaatga cggaaaacca gacatcatca ttgaactcgg tcctcactct gcactcgagg
     3001 gccctatcaa gcaagtttta aagggaatta gcaagcaggc tgctacaagc gtcaggtatc
     3061 attctgcttt gctccgcaac caagatgctg cggttacagc cgtcaagctt gccggtaatc
     3121 tttggaccaa aggccttgat atcgacttca gccaggtcaa tataacgttg gaagggcagt
     3181 tggagcccgg cctggtttca gacctacctt cataccagtg gtcaaaacat cagtgctgga
     3241 cagagtctcg cgccggcaaa tctcatcgcc ttaaggattt tggtcggcac gatctacttg
     3301 gactcctaca ggacgactgc aacgaaactg agcctgtctg gaccaatgtt cttgattctg
     3361 atgaagtgcc atggctcaag gatcataaga tgcagtctct tgtcacattc ccctgcgctg
     3421 ggtacctgac tatggcaacc gaagctgctt ctcaaacagc gcggatgcgt ggcgtgctgt
     3481 tggaagaaat tgcctcatac catatgcgaa acatccaggt tagcaagcct ttcatcctcg
     3541 atgatggcga gtcttatgac acacgcttta ctctcaagcg ttatgccgaa ggtctggcag
     3601 cgtattctaa cgactgggat gaatttcaat actcctcttg ggccccttcc cgaggctggg
     3661 ttgagcactg ccgtggcctc gttggcatca agaagcgcag caacaccaat attgttcgaa
     3721 tgaacaacca aaaagctgct attgctcgca gggccaatat gcagccacag cctggtactg
     3781 agatcgattg tgaatcgttc tactcagaac ttttgggtaa gggtgctgga tacaccagag
     3841 acttcacttt caaggatggt gcaaatctga gagtcgatgg tgattgttca gctggcatag
     3901 cagttctcca ggatgttgct gccaatatgc ctttcaacca tgagacacaa agcactctgc
     3961 cctctgcatt aacagacttg atatttcaaa catgtttctt gatcctcggt gcagggcgcg
     4021 gaaatctgaa gagcttggtc gtgccatcgg ctatcggaag tgtcgagatt tctaccacat
     4081 gcccaaatac tcccggcgag acgcttgaaa tcgtgaccca ccaccaaaac tttgcttcaa
     4141 aggaccgatt ggtatttgac agtgatgcat ggcacacaaa tgcgagtgag ccggtcgtta
     4201 tgatgcgaag caccatcttg acgcccattc atggtgatgc ttttgaaagt caagagccac
     4261 ggtctgcgtg ctatagagtg atctgggaac ctttggaagc ccaagttgca gccaaacttg
     4321 aagacaatgc cgcatcagat ggggcaaata accattccaa ctgtcacact aatgggcaca
     4381 gagatggaca cgccaatggt tatataaacg gccatgccaa cggcagcacc aatggacata
     4441 caaatggcca tattagtggg tatacaaacg gacacaccaa tggacacacc aacggcaacg
     4501 ccagcaacat caacggcaac accaacggcc atacaaatgg tcataccagt aggtatacca
     4561 atggacacac aaacggcaac accaacggcc atacaaatgg tcatacaaat ggacacacca
     4621 acgggcgtac caacggttat acaaacggcc gtaccaatgg acattcgaat ggtggctcta
     4681 atgggtatac caatggtgtg ccgacttctg gcgttgactt gagcgccact cccatcaccg
     4741 tcatttgcga tgctggtccc tcaaaccctc tctgcgctgc ccttgcggat ctgattgaga
     4801 ttcagacgtg tggatgcagg ccaaccatta tatcactagc aaaagcagag atatgttcat
     4861 ccgtcagata tgttgtcttg aatgaggtcg atagcccggt cctcgatgac atgaatgccg
     4921 atacatttgc tctgatccag aagctcctgc ttgatggctt gtctatcctc tgggtaagct
     4981 ctggtgctta tctaaatgca gagagcccac ataacaacat agcccaggga atgttccgaa
     5041 ccattcgttc cgagctgggt aaagtagcgg ccactcttga cctcgatacc aaatctaagt
     5101 tgcggcctga cgatcaggcg cagcttatca tcactgcaat gaagacatcc atggaaacat
     5161 tcaaagatga atccatcgat tatgaatttg cggagaaaga tgggaaactt ctcgttcccc
     5221 gtgtcgtaga agaaaatgag atgaatcttg acctcttccg ccaaacccag tctcctcccc
     5281 cttatctcca aaacttcaac gagcccaacc gccgcctcaa gatcgcggtt ggcacatacg
     5341 gtgcgctcga ctccctattc tgggacgacg aggagacata cgggcttggg ccaaacgaga
     5401 ttgaagttaa agttgctgct actggcatga acttcaaaga cgttgtcatt gccatgggac
     5461 aagtgcctag cccgtacctc ggaatcgagt gttccggtac cgtctcacgc attggatctg
     5521 gtgtcacctc gctcaagatt ggaaatcgag tttgtgctat gacacacggt gcttatagta
     5581 cttttgctcg ctgtccagct actagcgcag ctgtcatccc agaggatatt gcattctcca
     5641 cagccgcttc tatccccgtt atcttctgca cagcctacta tggcctgata gagcttgctc
     5701 accttgaacg tggggagaaa atattaattc acgcagcgtc tggcggcgtg ggacaggccg
     5761 ccattcagct ctcccagatg gtaggcgctg agatttttgc cactaccagc agtgttgaga
     5821 agaaaaacca tcttattgat acctatggta ttccggagga ccatatcttt tatagtagag
     5881 ataccagttt tgggcctgcc gtcagagaat ctaccgacgg gaaaggtgta gatgttgtaa
     5941 tcaactccct tgctggtgat ctgctgcgag aaacctgggc ctgcattgcc ccatttggcc
     6001 gctttattga aattggaaaa cgtgatatca cctccaacac tcgactggaa atggacaaat
     6061 ttgaatacaa ttgctctttc cactcggttg atttgtccaa ggttgctgac tggcgaccaa
     6121 agataatgag tcgagttttc aaggaagtca tggctctcat ctccaaaagg attatccagc
     6181 ccattagccc catcactgag attggcatct ctaaggtcga agctgcgttg cgcaaacttc
     6241 agagcggcaa gactaagggc aaggtcattg tcaaccatct cgtcaatgag caggtcaatg
     6301 ctgcccacag acagcctacc ggagacttag tgaatggaaa tgccacttat atcatcattg
     6361 gcggtacagg tggccttggt cgttccatgg caaagaaaat ggctcagcgc ggtggaaagc
     6421 acgtcgtcct tctatcccgc agcggcaaga tgactcctga gctagatcat ctcagcaaag
     6481 aatgcgagat atatggagcc aagatctatg taaaatgctg tgacgcggcc aaccaggcaa
     6541 aagtcaacga acttatctca gagctgcaat cgtcactgcc tccgatccgt ggtgtcatcc
     6601 atgcagccat ggtgctgcgg gataccctct ttgaaaagat gtcattcgag gactgggatg
     6661 aggttgtgcg ctgtaaggtt gcgggaggct ggaacttcca cattgcctta gcaaaggagc
     6721 ccctcgactt cttcatcgtc ctatcctcgg ttgctggcat tgttggcaac cgcggccagg
     6781 cagcctacgc cgctgccaac accttcttag atgccttgac agtccaccgc cgtaggttgg
     6841 gtcttaaagc tacatcgctc aacctaactg cagtcgagga tatcggttat ctggcggaga
     6901 attccggcaa gcaaggcgaa gttcttcgca atttatctgg taacggaatt ggcggggccg
     6961 aagtcctagc actcatcgag agttctataa acggcaaggt tggcaccgcc tgcgacgacc
     7021 agtgtatcac cggacttgaa ttcggggact cgatgcccta ctttgccgcg gatggcaagt
     7081 tcaagtatct tcgtcaggct gctcttgcgc tgagcgctgc tgcgggtgat gacggtggcg
     7141 cgaatattcc tattggcaag agactagcta gggcgatgac agtgggcgaa gccgtcgagc
     7201 ttgttaccga tggcttgcgt gacaaactgg gcgccattct gatgcttgac cccgatgtca
     7261 ttctagctaa acaggcgact gtcaccatgg ctgcccttgg cttggactca ctcaacgcta
     7321 tcgagctgcg taattggatc gcaagggacc tgcagacaca cttgcagatc ctggagctgc
     7381 tgcgagctgg aacgttggct gatcttgcaa gtcagattct gaagaagact cgcatccaag
     7441 gtgcttggac aatgcaggag ggagccagat aaggagattg ataagacttt tctagttgca
     7501 tatcttttat atttaaatct tatctattag ttaatttttt gtaatttatc cttatatata
     7561 gtctggttat tctaaaatat catttcagta tctaaaaatt cccctctttt ttcagttata
     7621 tcttaacagg cgacagtcca aatgttgatt tatcccagtc cgattcatca gggttgtgaa
     7681 gcattttgtc aatggtcgaa atcacatcag taatagtgcc tcttacttgc ctcatagaat
     7741 ttctttctct taacgtcacc gtttggtctt ttatagtttc gaaatctatg gtgataccaa
     7801 atggtgttcc caattcatcg ttacgggcgt attttttacc aattgaagta ttggaatcgt
     7861 caattttaaa gtatatctct cttttacgta aagcctgcga gatcctctta agtatagcgg
     7921 ggaagccatc gttattcgat attgtcgtaa caaatacttt gatcggcgct atgcggccgc
     7981 caccgcggtg gagctccagc ttttgttccc tttagtgagg gttaattgcg cgcttggcga
     8041 gactcaaaca agatatattt tgcccgcctt acagacgaaa ctagttggag gttatggagc
     8101 atactatcac gtgggcggcc actggtgagt tactacaccc caggggcaac gttgatgctc
     8161 ctaaaaaact ctggctggac gcaagccgta acacccgtgt cacttcataa tcgtttgcaa
     8221 ttcagggctt gatctacaca ttaccctgtt atccctatta attaatacgg ttatccacag
     8281 aatcagggga taacgcagga aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc
     8341 gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca
     8401 aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt
     8461 ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc
     8521 tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc
     8581 tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc
     8641 ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact
     8701 tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg
     8761 ctacagagtt cttgaagtgg tggcctaact acggctacac tagaaggaca gtatttggta
     8821 tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca
     8881 aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa
     8941 aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg
     9001 aaaactcacg ttaagggatt ttggtcatga gattatcaaa aaggatcttc acctagatcc
     9061 ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa acttggtctg
     9121 acagttacca atgcttaatc agtgaggcac ctatctcagc gatctgtcta tttcgttcat
     9181 ccatagttgc ctgactcccc gtcgtgtaga taactacgat acgggagggc ttaccatctg
     9241 gccccagtgc tgcaatgata ccgcgagacc cacgctcacc ggctccagat ttatcagcaa
     9301 taaaccagcc agccggaagg gccgagcgca gaagtggtcc tgcaacttta tccgcctcca
     9361 tccagtctat taattgttgc cgggaagcta gagtaagtag ttcgccagtt aatagtttgc
     9421 gcaacgttgt tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt
     9481 cattcagctc cggttcccaa cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa
     9541 aagcggttag ctccttcggt cctccgatcg ttgtcagaag taagttggcc gcagtgttat
     9601 cactcatggt tatggcagca ctgcataatt ctcttactgt catgccatcc gtaagatgct
     9661 tttctgtgac tggtgagtac tcaaccaagt cattctgaga atagtgtatg cggcgaccga
     9721 gttgctcttg cccggcgtca atacgggata ataccgcgcc acatagcaga actttaaaag
     9781 tgctcatcat tggaaaacgt tcttcggggc gaaaactctc aaggatctta ccgctgttga
     9841 gatccagttc gatgtaaccc actcgtgcac ccaactgatc ttcagcatct tttactttca
     9901 ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg
     9961 cgacacggaa atgttgaata ctcatactct tcctttttca atattattga agcatttatc
    10021 agggttattg tctcatgagc ggatacatat ttgaatgtat ttagaaaaat aaacaaatag
    10081 gggttccgcg cacatttccc cgaaaagtgc cacctgaacg aagcatctgt gcttcatttt
    10141 gtagaacaaa aatgcaacgc gagagcgcta atttttcaaa caaagaatct gagctgcatt
    10201 tttacagaac agaaatgcaa cgcgaaagcg ctattttacc aacgaagaat ctgtgcttca
    10261 tttttgtaaa acaaaaatgc aacgcgagag cgctaatttt tcaaacaaag aatctgagct
    10321 gcatttttac agaacagaaa tgcaacgcga gagcgctatt ttaccaacaa agaatctata
    10381 cttctttttt gttctacaaa aatgcatccc gagagcgcta tttttctaac aaagcatctt
    10441 agattacttt ttttctcctt tgtgcgctct ataatgcagt ctcttgataa ctttttgcac
    10501 tgtaggtccg ttaaggttag aagaaggcta ctttggtgtc tattttctct tccataaaaa
    10561 aagcctgact ccacttcccg cgtttactga ttactagcga agctgcgggt gcattttttc
    10621 aagataaagg catccccgat tatattctat accgatgtgg attgcgcata ctttgtgaac
    10681 agaaagtgat agcgttgatg attcttcatt ggtcagaaaa ttatgaacgg tttcttctat
    10741 tttgtctcta tatactacgt ataggaaatg tttacatttt cgtattgttt tcgattcact
    10801 ctatgaatag ttcttactac aatttttttg tctaaagagt aatactagag ataaacataa
    10861 aaaatgtaga ggtcgagttt agatgcaagt tcaaggagcg aaaggtggat gggtaggtta
    10921 tatagggata tagcacagag atatatagca aagagatact tttgagcaat gtttgtggaa
    10981 gcggtattcg caatatttta gtagctcgtt acagtccggt gcgtttttgg ttttttgaaa
    11041 gtgcgtcttc agagcgcttt tggttttcaa aagcgctctg aagttcctat actttctaga
    11101 gaataggaac ttcggaatag gaacttcaaa gcgtttccga aaacgagcgc ttccgaaaat
    11161 gcaacgcgag ctgcgcacat acagctcact gttcacgtcg cacctatatc tgcgtgttgc
    11221 ctgtatatat atatacatga gaagaacggc atagtgcgtg tttatgctta aatgcgtact
    11281 tatatgcgtc tatttatgta ggatgaaagg tagtctagta cctcctgtga tattatccca
    11341 ttccatgcgg ggtatcgtat gcttccttca gcactaccct ttagctgttc tatatgctgc
    11401 cactcctcaa ttggattagt ctcatccttc aatgctatca tttcctttga tattggatca
    11461 tactaagaaa ccattattat catgacatta acctataaaa ataggcgtat cacgaggccc
    11521 tttcgtctcg cgcgtttcgg tgatgacggt gaaaacctcg gcgcgccatt accctgttat
    11581 ccctaatata caacgcaaag aagctggaaa attggcgagg gaatcctgtt tctgtctatc
    11641 caagaatggg catgaggtgg caaccgtcgt gctagcgtac agggtgcact ttgtaaccat
    11701 ttgggacacc ggacactcgc tgttttcgaa attacccttt aagcgcgggt attgaaccag
    11761 gcttatgccc aagatcgtag caagcattta actgtgggaa tactcaggta tcgtaagatg
    11821 caagagttcg aatctcttag caaccattat ttttttcctc aacataacga gaacacacag
    11881 gggcgctatc gcacagaatc aaattcgatg actggaaatt ttttgttaat ttcagaggtc
    11941 gcctgacgca tatacctttt tcaactgaaa aattgggaga aaaaggaaag gtgagaggcc
    12001 ggaaccggct tttcatatag aatagagaag cgttcatgac taaatgcttg catcacaata
    12061 cttgaagttg acaatattat ttaaggacct attgtttttt ccaataggtg gttagcaatc
    12121 gtcttacttt ctaacttttc ttacctttta catttcagca atatatatat atatttcaag
    12181 gatataccat tctaatgtct gcccctatgt ctgcccctaa gaagatcgtc gttttgccag
    12241 gtgaccacgt tggtcaagaa atcacagccg aagccattaa ggttcttaaa gctatttctg
    12301 atgttcgttc caatgtcaag ttcgatttcg aaaatcattt aattggtggt gctgctatcg
    12361 atgctacagg tgtcccactt ccagatgagg cgctggaagc ctccaagaag gttgatgccg
    12421 ttttgttagg tgctgtggct ggtcctaaat ggggtaccgg tagtgttaga cctgaacaag
    12481 gtttactaaa aatccgtaaa gaacttcaat tgtacgccaa cttaagacca tgtaactttg
    12541 catccgactc tcttttagac ttatctccaa tcaagccaca atttgctaaa ggtactgact
    12601 tcgttgttgt cagagaatta gtgggaggta tttactttgg taagagaaag gaagacgatg
    12661 gtgatggtgt cgcttgggat agtgaacaat acaccgttcc agaagtgcaa agaatcacaa
    12721 gaatggccgc tttcatggcc ctacaacatg agccaccatt gcctatttgg tccttggata
    12781 aagctaatct tttggcctct tcaagattat ggagaaaaac tgtggaggaa accatcaaga
    12841 acgaattccc tacattgaag gttcaacatc aattgattga ttctgccgcc atgatcctag
    12901 ttaagaaccc aacccaccta aatggtatta taatcaccag caacatgttt ggtgatatca
    12961 tctccgatga agcctccgtt atcccaggtt ccttgggttt gttgccatct gcgtccttgg
    13021 cctctttgcc agacaagaac accgcatttg gtttgtacga accatgccac ggttctgctc
    13081 cagatttgcc aaagaataag gttgacccta tcgccactat cttgtctgct gcaatgatgt
    13141 tgaaattgtc attgaacttg cctgaagaag gtaaggccat tgaagatgca gttaaaaagg
    13201 ttttggatgc aggtatcaga actggtgatt taggtggttc caacagtacc accgaagtcg
    13261 gtgatgctgt cgccgaagaa gttaagaaaa tccttgctta aaaagattct ctttttttat
    13321 gatatttgta cataaacttt ataaatgaaa ttcataatag aaacgacacg aaattacaaa
    13381 atggaatatg ttcatagggt agacgaaact atatacgcaa tctacataca tttatcaaga
    13441 aggagaaaaa ggaggatagt aaaggaatac aggtaagcaa attgatacta atggctcaac
    13501 gaaatgacgg tatcgataag cttgatatcg aattcctgca gcccggggga tccactagtt
    13561 ctagagttaa ttaa
//
