LOCUS       sequence-208200-       12066 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of a fungal biosynthetic gene cluster.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12066)
  AUTHORS   Harvey CJB, Tang M, Schlecht U, Horecka J, Fischer CR, Lin HC, Li J,
            Naughton B, Cherry J, Miranda M, Li YF, Chu AM, Hennessy JR, Vandova
            GA, Inglis D, Aiyar RS, Steinmetz LM, Davis RW, Medema MH, Sattely 
            E, Khosla C, St Onge RP, Tang Y, Hillenmeyer ME
  TITLE     HEx: A heterologous expression platform for the discovery of fungal 
            natural products.
  JOURNAL   Sci Adv. 2018 Apr 11;4(4):eaar5459. doi: 10.1126/sciadv.aar5459. 
            eCollection 2018 Apr.
  PUBMED    29651464
REFERENCE   2  (bases 1 to 12066)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Adv."; 
            date: "2018-04-11"; volume: "4(4):eaar5459. doi"; pages: " 
            10.1126/sciadv.aar5459. eCollection 2018 Apr"
COMMENT     Sequence Label: pCHSU62.2-2.2
FEATURES             Location/Qualifiers
     source          1..12066
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      1263..1562
                     /label=ADH2 terminator
                     /note="transcription terminator for the S. cerevisiae 
                     alcohol dehydrogenase 2 (ADH2) gene"
     promoter        4471..4691
                     /gene="S. cerevisiae URA3"
                     /label=URA3 promoter
     CDS             4692..5495
                     /codon_start=1
                     /gene="S. cerevisiae URA3"
                     /product="orotidine-5'-phosphate decarboxylase, required 
                     for uracil biosynthesis"
                     /label=URA3
                     /note="yeast auxotrophic marker, counterselectable with 
                     5-fluoroorotic acid (5-FOA)"
                     /translation="MSKATYKERAATHPSPVAAKLFNIMHEKQTNLCASLDVRTTKELL
                     ELVEALGPKICLLKTHVDILTDFSMEGTVKPLKALSAKYNFLLFEDRKFADIGNTVKLQ
                     YSAGVYRIAEWADITNAHGVVGPGIVSGLKQAAEEVTKEPRGLLMLAELSCKGSLSTGE
                     YTKGTVDIAKSDKDFVIGFIAQRDMGGRDEGYDWLIMTPGVGLDDKGDALGQQYRTVDD
                     VVSTGSDIIIVGRGLFAKGRDAKVEGERYRKAGWEAYLRRCGQQN"
     primer_bind     complement(8213..8233)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(8213..8231)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     rep_origin      complement(8570..9158)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8650..8669)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9329..10120,10121..10189))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9952..9971
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(10190..10294)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      10321..11663
                     /label=2u ori
                     /note="yeast 2u plasmid origin of replication"
     protein_bind    complement(11284..11331)
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     primer_bind     11704..11722
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(12041..12057)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(12041..12057)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
ORIGIN
        1 tatctaaaaa ttgccttatg atccgtctct ccggttacag cctgtgtaac tgattaatcc
       61 tgcctttcta atcaccattc taatgtttta attaagggat tttgtcttca ttaacggctt
      121 tcgctcataa aaatgttatg acgttttgcc cgcaggcggg aaaccatcca cttcacgaga
      181 ctgatctcct ctgccggaac accgggcatc tccaacttat aagttggaga aataagagaa
      241 tttcagattg agagaatgaa aaaaaaaaaa aaaaaaaagg cagaggagag catagaaatg
      301 gggttcactt tttggtaaag ctatagcatg cctatcacat ataaatagag tgccagtagc
      361 gacttttttc acactcgaaa tactcttact actgctctct tgttgttttt atcacttctt
      421 gtttcttctt ggtaaataga atatcaagct acaaaaagca tacaatcaac tatcaactat
      481 taactatatc gtaatacaca atgaagatcc tctgtatcca tggctcgtat ggcagcgcat
      541 ctgcctttcg cacccagctc gaaccctttc tcgcccagat agagaagcct ggggaggtgg
      601 atttcaaatt catcgacggc ctctatcctg ccgtgcctcc agatggcttt caggactact
      661 ttggcccgcc acctcacttc ctcaacgttc aatacgacgg tattgctggc ttagagaaca
      721 tgcaattgaa cctgcgtaca ctcaaaccag gagaaagctt cgaagacaca ataaagaggc
      781 ttatcaaaaa tgatgccaca gtcgtgtcta aagaagaata tatgaagacc ctcgactaca
      841 ttgagcagtg cctggtcgag gatccagaaa ttcagggaat tcttggttac tccgaaggtg
      901 ctacgactgc tgcaacatat ttgttacagc aggcacaccg gcaccgagac gaaggcatcc
      961 cacacagggt caagtttggc atcttcttcg ggggctggcc gcctgcaaag ttcgaaggtg
     1021 accgccttgc gcccgtccta cccgaggacg atgatatgat tgatatccca acatgccaca
     1081 ttgtcggctg tagcgacccc tatctttacg gcgccatgac cctgtttcag gtctgcgatg
     1141 aagagacagc caccctattc gatcatggca agggtcattt gctgcctcga gatcccatga
     1201 ccattgccga gttatcagaa tgcatcaaca caactctcaa gcaggcaggt ttccttaact
     1261 aggcggatct cttatgtctt tacgatttat agttttcatt atcaagtatg cctatattag
     1321 tatatagcat ctttagatga cagtgttcga agtttcacga ataaaagata atattctact
     1381 ttttgctccc accgcgtttg ctagcacgag tgaacaccat ccctcgcctg tgagttgtac
     1441 ccattcctct aaactgtaga catggtagct tcagcagtgt tcgttatgta cggcatcctc
     1501 caacaaacag tcggttatag tttgtcctgc tcctctgaat cgtctccctc gatatttctc
     1561 attttccttc gcatgccagc attgaaatga tcgaagttca atgatgaaac ggtaattctt
     1621 ctgtcattta ctcatctcat ctcatcaagt tatataattc tatacggatg taatttttca
     1681 cttttcgtct tgacgtccac cctataattt caattattga accctcacga tccagttctc
     1741 cagtgacaca gcctttatct ggtcaaacct ttctttctaa tcacctatgc tgatgcttaa
     1801 ttaagggatt tttgtctcca tcaacggcat gcgcccaaaa atgacgtttt ttttaaccca
     1861 tagacacgaa actacccatt ttccaccggc ctgacctacc accggaacaa cggccatctc
     1921 caacttgcaa gttggggaaa ttaagagcat cgcaggttta atggaagaaa aaaaaaaggt
     1981 acagcacagc gcaaatggag ttagttccct tatgtcacac actcacacac agtcggtcag
     2041 atcaagcata ctgggtgcgt ataaatagag tggccattgc caccctgttt atctcaaaat
     2101 ctgtcttgtt agtggtcttc tccctttttc aggttacaat tctcttgttt ctacttagta
     2161 tataagtata tcaagctata ttaagcatac tatcaactgt caactctatc ctcaaaatac
     2221 aatacaaaat ggacgacttg ctggctctcg tcgagggtgg ccctgcgctc aagccgacga
     2281 tcaactcact ctggacagcc ctgcaccagc gtgccagcat acagccagac agactggccc
     2341 tgctgagccc ccggcagcct ggaaacttgc tcgaaggact cgtccggcct agaggaagga
     2401 gagatgagag tggcagccgc gatgccctca tgcgtagcct caagcgagca gccacaccgg
     2461 cgtacatgct cagctacctg ctatcgtggg ttctgccggc gcctgataca gagacagact
     2521 acctcagatg gtcctttgca gagctccaac gtgcaacaat tcgtctagct tcttttctgc
     2581 gtcgtcgtgg tgtgaagcca ggggcgacag gcgccttctt caccccggtc tgcgcagaat
     2641 gggcgttgat gctgtctgtg tctgcgtaca gtcgctacac gggtgtgacg ctcgaccgga
     2701 agacgctcgc gcctggtaat gagcacgacc tgcgagtaca tatgaacaca ctaagcccgt
     2761 ctttcattat cgttactacg gtagaggaag cgctgcatct tgctggacag acagatgtag
     2821 cactcgggat ctgcttggag aacttcgcga cgcctgctcc ggagggctgg ctgagcatgg
     2881 cagatattgc agcatcctca tatgaagagg aagaggaaga agaagtagaa gaagcagaag
     2941 aagtggaaga aaagagagaa agtgacagga cagccttcgt tgtgtttact agtggctcgt
     3001 ccggcatacc caagggctgc ccagtgtccg ccagccaaat tctatctctt ctcggagctg
     3061 tgtccaagca gatgcctctc ctaccaactc ctatgactct tgtctcgggc acatgcttcc
     3121 ggtcgctctg cacagccatc acactgatgt cctgggcctc aggcaacgcc gccgtcatgg
     3181 acgccagcaa cgaggcgaca gctgagacga agctcgctgg gctgcaaaca tgccgacctg
     3241 cctttgcctc cgtaaccgtc acggcgctct cctctattgc ccgtagccca aactacaccg
     3301 ccgacgccat caggtcggtc cgcttcgtcc atctcatcgg ctcaactatg accgcgtcgg
     3361 tcctccgaag ggccaagaag acgttccctc acgcaacgat cctgcccagc tttggcatgt
     3421 cagaggccgc aaagacagtg acctgggaag ctgccggagg tgtgccatcc gttgacaaga
     3481 tacctacatg gcacggcatc gccgcctcag gcaaggtggc tcccggcgaa aggctcaagg
     3541 tcgttgatat agacggtaac gttgcacgcc gtaatcatgt tggtgcgtta cacctgagcg
     3601 gcgactcagt tatcggtggt tacttgggtg gtgccaattc tgctgccttt tacgtaggag
     3661 acgacggcca ccactggttc gtctctgggg acgatgccgt gattgatgac caaggccttc
     3721 tctatgtcct cggtcgaagt gagtatactc tcaggcgcga cagcaagctc atcctcccct
     3781 gtatcgttga aaattttctc gagacgcaat tcgaagggtc ctcggctgcc gtagtgaata
     3841 tcaccaccag caacggtgat aggggtttat ttgctgtctt agagcacgct gtccctgccc
     3901 ctgcatccgc tgctgttcaa gaacttgtcg ctcaaaagct tggcttggaa taccgtgtcg
     3961 aaggtgctgt gagcctcaat gagctaggat tcgagaagtg gccgctgacc tcgggagaaa
     4021 aagtcgccta ccgtgattta cggctcgcca ttgaggaatt tctcacttcc aagacggcaa
     4081 atttatgaac aaatcgctct taaatatata cctaaagaac attaaagcta tattataagc
     4141 aaagatacgt aaattttgct tatattatta tacacatatc atatttctat atttttaaga
     4201 tttggttata taatgtacgt aatgcaaagg aaataaattt tatacattat tgaacagcgt
     4261 ccaagtaact acattatgtg cactaatagt ttagcgtcgt gaagacttta ttgtgtcgcg
     4321 aaaagtaaaa attttaaaaa ttagagcacc ttgaacttgc gaaaaaggtt ctcatcaact
     4381 gtttaaaagg aggatatcag gtcctatttc tgacaaacaa tatacaaatt tagtttcaaa
     4441 gatgaatcag tgcgcgaagg acataactca ttcaattcaa ttcatcattt tttttttatt
     4501 cttttttttg atttcggttt ctttgaaatt tttttgattc ggtaatctcc gaacagaagg
     4561 aagaacgaag gaaggagcac agacttagat tggtatatat acgcatatgt agtgttgaag
     4621 aaacatgaaa ttgcccagta ttcttaaccc aactgcacag aacaaaaacc tgcaggaaac
     4681 gaagataaat catgtcgaaa gctacatata aggaacgtgc tgctactcat cctagtcctg
     4741 ttgctgccaa gctatttaat atcatgcacg aaaagcaaac aaacttgtgt gcttcattgg
     4801 atgttcgtac caccaaggaa ttactggagt tagttgaagc attaggtccc aaaatttgtt
     4861 tactaaaaac acatgtggat atcttgactg atttttccat ggagggcaca gttaagccgc
     4921 taaaggcatt atccgccaag tacaattttt tactcttcga agacagaaaa tttgctgaca
     4981 ttggtaatac agtcaaattg cagtactctg cgggtgtata cagaatagca gaatgggcag
     5041 acattacgaa tgcacacggt gtggtgggcc caggtattgt tagcggtttg aagcaggcgg
     5101 cagaagaagt aacaaaggaa cctagaggcc ttttgatgtt agcagaattg tcatgcaagg
     5161 gctccctatc tactggagaa tatactaagg gtactgttga cattgcgaag agcgacaaag
     5221 attttgttat cggctttatt gctcaaagag acatgggtgg aagagatgaa ggttacgatt
     5281 ggttgattat gacacccggt gtgggtttag atgacaaggg agacgcattg ggtcaacagt
     5341 atagaaccgt ggatgatgtg gtctctacag gatctgacat tattattgtt ggaagaggac
     5401 tatttgcaaa gggaagggat gctaaggtag agggtgaacg ttacagaaaa gcaggctggg
     5461 aagcatattt gagaagatgc ggccagcaaa actaaaaaac tgtattataa gtaaatgcat
     5521 gtatactaaa ctcacaaatt agagcttcaa tttaattata tcagttatta ccctatgcgg
     5581 tgtgaaatac cgcacagatg cgtaaggaga aaataccgca tcaggatagg aaaaaaccga
     5641 gcttcctttc atccggcgcg gctgtgttct acatatcact gaagctccgg gtattttaag
     5701 ttatacaagg gaaagatgcc ggctagacta gcaagtttta ggctgcttaa cattatggat
     5761 aggcggataa agggcccaaa caggattgta aagcttagac gcttctggtt ggacaatggt
     5821 acgtttgtgt attaagtaag gcttggctgg ggatagcaac attgggcaga gtatagaaga
     5881 ccacaaaaaa aaggtatata agggcagaga agtctttgta atgtgtgtaa cttctcttcc
     5941 atgtgtaatc agtatttcta cttacttctt aaatatacag aagtaagaca gataaccaac
     6001 agcctttccc agatatacat atatatcttt atttcagctt aaacaataat tatatttgtt
     6061 taactcaaaa ataaaaaaaa aaaaccaaac tcacgcaact aattattcca taataaaata
     6121 acaacatgcc gcgcttcaac cccctaagca tctcctgtct ggctactctg ctgaccacct
     6181 accttggtac agcatctgta tctgcacagt caccacccca ggaaaccctc tggccggtgc
     6241 agacgttcca gagtacggat atccagacgc cgtttttcaa tgtgacgaag aaaggccaga
     6301 cggaaccggg ctatatcttc ataagcccga gagataaggc ccgaaacatt ggacatccag
     6361 ctatcctcga ggacaatggc caggttgtct ggaagggacc ggaagacgcg agcacatatg
     6421 gcttcaagcc tcagcttctt gacgggaagc cagtgctggt tagctggttc gggttcgtga
     6481 acgagactgg attcggactg ggatctatga gcatcttcga cagctcgtat gagaagatcc
     6541 acgaggtgat tctgcccggt ggtgataacg aatactacaa gacgatttat gagcccatga
     6601 cgttcccatc atacatggac aaccacgaag gccagatcac ggaccaagga accatcgttg
     6661 tgactgttgt caatgtcact gaggcagatc tccgtgctgt tggcgggcct gagaagggct
     6721 gggttgtcga cggaggcttt ttggaaatgg acatcaagac caacgaagtc ctcttccgct
     6781 ggaatgccgc tgagcacatt gatgagatcc ctgtcacttt ctcccttaag ccaattgagg
     6841 gaactggaac ttcgcccaaa aacccatgga actacgtcca tgtcaactcc gtcttcaaat
     6901 ccggagactc ttatatcgtc tcttctcggt actcctgcaa catcttcctc atctccaagg
     6961 aaggcgagat tgtctggcgc ctcaacggca ttgatggagg tgacttcgag ctaggccccg
     7021 gcgccaactt ctgttaccag catgaagtca gggtcgaatc ccacaccgtg gacaagatca
     7081 cgctgactac acacaacaac gacaacgccg acttcacgcc tgccaatgaa accaaaccga
     7141 caaccggtct ggtcctcgat ctcgatctca acgccaagaa agtcactttg aaccgcatgg
     7201 tatggaactc cgagcggcct gtcgtctctc ggggccaggg gagctaccag gtgcttggca
     7261 acgaccacgt cctgatgggc cagggcgcca tccccatcgt agaggagtac gatgccaacg
     7321 gcgccgtcgt aatggacgcc cgtttcggca acgacggcgt caccaactgc taccgcgcct
     7381 accgcagcgc ctgggttggc acacccaaga cgaaaccgag cgtaaaggcc tgcggtgccg
     7441 atccgaacgg cgcggtggtg ttttatgtga gctggaacgg cgcgacggac gtagagtcgt
     7501 ggaagatttt ctctgtctcg gactctggcg agcacagtga gatagctaat gtgccgaaaa
     7561 atggctttga gacgaggatg gagctgcaga aggctggcag caagttcatc gtccagtcgg
     7621 ttggaggacc taacgacggg gtgcagtctg atgttgtcat gggcgaaaac tgctagggag
     7681 attgataaga cttttctagt tgcatatctt ttatatttaa atcttatcta ttagttaatt
     7741 ttttgtaatt tatccttata tatagtctgg ttattctaaa atatcatttc agtatctaaa
     7801 aattcccctc ttttttcagt tatatcttaa caggcgacag tccaaatgtt gatttatccc
     7861 agtccgattc atcagggttg tgaagcattt tgtcaatggt cgaaatcaca tcagtaatag
     7921 tgcctcttac ttgcctcata gaatttcttt ctcttaacgt caccgtttgg tcttttatag
     7981 tttcgaaatc tatggtgata ccaaatggtg ttcccaattc atcgttacgg gcgtattttt
     8041 taccaattga agtattggaa tcgtcaattt taaagtatat ctctctttta cgtaaagcct
     8101 gcgagatcct cttaagtata gcggggaagc catcgttatt cgatattgtc gtaacaaata
     8161 ctttgatcgg cgctatgcgg ccgccaccgc ggtggagctc cagcttttgt tccctttagt
     8221 gagggttaat tgcgcgcttg gcgagactca aacaagatat attttgcccg ccttacagac
     8281 gaaactagtt ggaggttatg gagcatacta tcacgtgggc ggccactggt gagttactac
     8341 accccagggg caacgttgat gctcctaaaa aactctggct ggacgcaagc cgtaacaccc
     8401 gtgtcacttc ataatcgttt gcaattcagg gcttgatcta cacattaccc tgttatccct
     8461 attaattaat acggttatcc acagaatcag gggataacgc aggaaagaac atgtgagcaa
     8521 aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc
     8581 tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga
     8641 caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc
     8701 cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt
     8761 ctcatagctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct
     8821 gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg
     8881 agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta
     8941 gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct
     9001 acactagaag gacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa
     9061 gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt
     9121 gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta
     9181 cggggtctga cgctcagtgg aacgaaaact cacgttaagg gattttggtc atgagattat
     9241 caaaaaggat cttcacctag atccttttaa attaaaaatg aagttttaaa tcaatctaaa
     9301 gtatatatga gtaaacttgg tctgacagtt accaatgctt aatcagtgag gcacctatct
     9361 cagcgatctg tctatttcgt tcatccatag ttgcctgact ccccgtcgtg tagataacta
     9421 cgatacggga gggcttacca tctggcccca gtgctgcaat gataccgcga gacccacgct
     9481 caccggctcc agatttatca gcaataaacc agccagccgg aagggccgag cgcagaagtg
     9541 gtcctgcaac tttatccgcc tccatccagt ctattaattg ttgccgggaa gctagagtaa
     9601 gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt
     9661 cacgctcgtc gtttggtatg gcttcattca gctccggttc ccaacgatca aggcgagtta
     9721 catgatcccc catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca
     9781 gaagtaagtt ggccgcagtg ttatcactca tggttatggc agcactgcat aattctctta
     9841 ctgtcatgcc atccgtaaga tgcttttctg tgactggtga gtactcaacc aagtcattct
     9901 gagaatagtg tatgcggcga ccgagttgct cttgcccggc gtcaatacgg gataataccg
     9961 cgccacatag cagaacttta aaagtgctca tcattggaaa acgttcttcg gggcgaaaac
    10021 tctcaaggat cttaccgctg ttgagatcca gttcgatgta acccactcgt gcacccaact
    10081 gatcttcagc atcttttact ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa
    10141 atgccgcaaa aaagggaata agggcgacac ggaaatgttg aatactcata ctcttccttt
    10201 ttcaatatta ttgaagcatt tatcagggtt attgtctcat gagcggatac atatttgaat
    10261 gtatttagaa aaataaacaa ataggggttc cgcgcacatt tccccgaaaa gtgccacctg
    10321 aacgaagcat ctgtgcttca ttttgtagaa caaaaatgca acgcgagagc gctaattttt
    10381 caaacaaaga atctgagctg catttttaca gaacagaaat gcaacgcgaa agcgctattt
    10441 taccaacgaa gaatctgtgc ttcatttttg taaaacaaaa atgcaacgcg agagcgctaa
    10501 tttttcaaac aaagaatctg agctgcattt ttacagaaca gaaatgcaac gcgagagcgc
    10561 tattttacca acaaagaatc tatacttctt ttttgttcta caaaaatgca tcccgagagc
    10621 gctatttttc taacaaagca tcttagatta ctttttttct cctttgtgcg ctctataatg
    10681 cagtctcttg ataacttttt gcactgtagg tccgttaagg ttagaagaag gctactttgg
    10741 tgtctatttt ctcttccata aaaaaagcct gactccactt cccgcgttta ctgattacta
    10801 gcgaagctgc gggtgcattt tttcaagata aaggcatccc cgattatatt ctataccgat
    10861 gtggattgcg catactttgt gaacagaaag tgatagcgtt gatgattctt cattggtcag
    10921 aaaattatga acggtttctt ctattttgtc tctatatact acgtatagga aatgtttaca
    10981 ttttcgtatt gttttcgatt cactctatga atagttctta ctacaatttt tttgtctaaa
    11041 gagtaatact agagataaac ataaaaaatg tagaggtcga gtttagatgc aagttcaagg
    11101 agcgaaaggt ggatgggtag gttatatagg gatatagcac agagatatat agcaaagaga
    11161 tacttttgag caatgtttgt ggaagcggta ttcgcaatat tttagtagct cgttacagtc
    11221 cggtgcgttt ttggtttttt gaaagtgcgt cttcagagcg cttttggttt tcaaaagcgc
    11281 tctgaagttc ctatactttc tagagaatag gaacttcgga ataggaactt caaagcgttt
    11341 ccgaaaacga gcgcttccga aaatgcaacg cgagctgcgc acatacagct cactgttcac
    11401 gtcgcaccta tatctgcgtg ttgcctgtat atatatatac atgagaagaa cggcatagtg
    11461 cgtgtttatg cttaaatgcg tacttatatg cgtctattta tgtaggatga aaggtagtct
    11521 agtacctcct gtgatattat cccattccat gcggggtatc gtatgcttcc ttcagcacta
    11581 ccctttagct gttctatatg ctgccactcc tcaattggat tagtctcatc cttcaatgct
    11641 atcatttcct ttgatattgg atcatactaa gaaaccatta ttatcatgac attaacctat
    11701 aaaaataggc gtatcacgag gccctttcgt ctcgcgcgtt tcggtgatga cggtgaaaac
    11761 ctcggcgcgc cattaccctg ttatccctaa tatacaacgc aaagaagctg gaaaattggc
    11821 gagggaatcc tgtttctgtc tatccaagaa tgggcatgag gtggcaaccg tcgtgctagc
    11881 gtacagggtg cactttgtaa ccatttggga caccggacac tcgctgtttt cgaaattacc
    11941 ctttaagcgc gggtattgaa ccaggcttat gcccaagatc gtagcaagca tttaaatgac
    12001 ggtatcgata agcttgatat cgaattcctg cagcccgggg gatccactag ttctagagtt
    12061 aattaa
//
