LOCUS       sequence-208201-       11993 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of a fungal biosynthetic gene cluster.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11993)
  AUTHORS   Harvey CJB, Tang M, Schlecht U, Horecka J, Fischer CR, Lin HC, Li J,
            Naughton B, Cherry J, Miranda M, Li YF, Chu AM, Hennessy JR, Vandova
            GA, Inglis D, Aiyar RS, Steinmetz LM, Davis RW, Medema MH, Sattely 
            E, Khosla C, St Onge RP, Tang Y, Hillenmeyer ME
  TITLE     HEx: A heterologous expression platform for the discovery of fungal 
            natural products.
  JOURNAL   Sci Adv. 2018 Apr 11;4(4):eaar5459. doi: 10.1126/sciadv.aar5459. 
            eCollection 2018 Apr.
  PUBMED    29651464
REFERENCE   2  (bases 1 to 11993)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Adv."; 
            date: "2018-04-11"; volume: "4(4):eaar5459. doi"; pages: " 
            10.1126/sciadv.aar5459. eCollection 2018 Apr"
COMMENT     Sequence Label: pCHSU65.1-2.2
FEATURES             Location/Qualifiers
     source          1..11993
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             504..527
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     primer_bind     complement(6428..6448)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(6428..6446)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     rep_origin      complement(6785..7373)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6865..6884)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7544..8335,8336..8404))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8167..8186
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8405..8509)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      8536..9878
                     /label=2u ori
                     /note="yeast 2u plasmid origin of replication"
     protein_bind    complement(9499..9546)
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     primer_bind     9919..9937
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        10209..10613
                     /gene="S. cerevisiae LEU2"
                     /label=LEU2 promoter
     CDS             10626..11720
                     /codon_start=1
                     /gene="S. cerevisiae LEU2"
                     /product="3-isopropylmalate dehydrogenase, required for 
                     leucine biosynthesis"
                     /label=LEU2
                     /note="yeast auxotrophic marker"
                     /translation="MSAPKKIVVLPGDHVGQEITAEAIKVLKAISDVRSNVKFDFENHL
                     IGGAAIDATGVPLPDEALEASKKVDAVLLGAVAGPKWGTGSVRPEQGLLKIRKELQLYA
                     NLRPCNFASDSLLDLSPIKPQFAKGTDFVVVRELVGGIYFGKRKEDDGDGVAWDSEQYT
                     VPEVQRITRMAAFMALQHEPPLPIWSLDKANLLASSRLWRKTVEETIKNEFPTLKVQHQ
                     LIDSAAMILVKNPTHLNGIIITSNMFGDIISDEASVIPGSLGLLPSASLASLPDKNTAF
                     GLYEPCHGSAPDLPKNKVDPIATILSAAMMLKLSLNLPEEGKAIEDAVKKVLDAGIRTG
                     DLGGSNSTTEVGDAVAEEVKKILA"
     primer_bind     complement(11968..11984)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(11968..11984)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
ORIGIN
        1 tatctaaaaa ttgccttatg atccgtctct ccggttacag cctgtgtaac tgattaatcc
       61 tgcctttcta atcaccattc taatgtttta attaagggat tttgtcttca ttaacggctt
      121 tcgctcataa aaatgttatg acgttttgcc cgcaggcggg aaaccatcca cttcacgaga
      181 ctgatctcct ctgccggaac accgggcatc tccaacttat aagttggaga aataagagaa
      241 tttcagattg agagaatgaa aaaaaaaaaa aaaaaaaagg cagaggagag catagaaatg
      301 gggttcactt tttggtaaag ctatagcatg cctatcacat ataaatagag tgccagtagc
      361 gacttttttc acactcgaaa tactcttact actgctctct tgttgttttt atcacttctt
      421 gtttcttctt ggtaaataga atatcaagct acaaaaagca tacaatcaac tatcaactat
      481 taactatatc gtaatacaca atggattata aggatgatga tgataagact agtatgaacg
      541 ccatctattt cagcaacgaa ttccccaagg aggatttgaa ggatgtcttc cgacagctgc
      601 acaaggagag caagagcagc atccaccatc tgctcgcacg ctttatgagt gaggccacga
      661 gggctgtgaa gaaggagatt gagcagctac cgtccagcct gaagcagata atacctcctt
      721 ttgagactct cagtgcttgg gcggagcaga aggaactccg cgaaggtgaa ctgtgtggcg
      781 ctatcgatgg cgttttacta gttctacttc agatatcagt atacatatgc catgctgagg
      841 ccggtgcaga gctcccaact gctccgcacc tatctgcgct tggaattgga ctcctcggag
      901 ccgccgctgt ttccatgtcc aagcagatct ctgacctgcc ctcctacggt gctcaagcta
      961 tcgctattgc tttccggttg ggtgttcacg ttcagggtgt gtctcaccag cttgaggccc
     1021 gcgagctcga cgagactccc aagacttggg cttatgttgt tcacaacatc gaccccaagg
     1081 atgctcaggc tgagctggag gcattccacg aggcgaactt gacatcaaac actgcccgca
     1141 tatttatcag cgccgttagc cgtacctccg ttacgatcag tggcccgccc tcgaagctcg
     1201 agagcctctt caagaagtcc aaggttttcc gggatgcacg atcaatcgcc ctgcccgtct
     1261 atggcggcct ttgccatgct cctagtgtct acagcacaaa ggacacacaa gccatcatcg
     1321 acggcttgaa cacgccgaca tctgcagctt tgtccagcca agctccccaa gcaccgctgt
     1381 actctacgag caccggaaag ccatatgagg cagtcaacgc catcgggctg ttctcatgcg
     1441 tcgtgaccga actattgacc aaagccatcg agtgggatca agtcattgcc ggtctagtca
     1501 gtgctgctaa gtcggctgaa gcaactgagg ttacgctctc ctgcttcggc aactcgattc
     1561 cactgaacga cctcaagaca gcgcttcaag ctgccatcgc cgatgtcaag atcaccattc
     1621 acagcttcat gaccgttctc aagaagaacg caactggcgg tgctcctccc cgcggtacag
     1681 cccagtcgaa gcttgccatt gttggaatgt catgcaggct tcctggtggc gccaccgaca
     1741 ctgagaagtt ctgggagctt cttgaagccg gtctagatgt ctctcgcaag attccggctt
     1801 ctcgctttga cattgaaacc cattacgatc cagagggtaa ggacttgaac aagaccatga
     1861 ctcaatacgg ttgctttatc gatgagcccg gcatgttcga tcccagcttc ttcaacatgt
     1921 ctccccgtga agccatggct gttgatcccc agatgcgcct gtctctcgtc actgcctatg
     1981 aagctttgga gaaagctggc tttgttggca accgcactct ttccaccaag ctccagcgca
     2041 tcggaacatt ctacggacag gcagctgacg attaccgtga ggtgaaccag ggtcaagagg
     2101 ttggcactta ctacatccct ggtggatgcc gtgccttcgg acctggtcgc atcaactact
     2161 tcttcaagtt tgccggacct agctacagcg ttgatactgc ttgctcttct gggcttgcag
     2221 ctatcgaggt tgcttgccag gctctgtgga acggcgaggt tgacatggct gtcgctggtg
     2281 gtgtcaacat cctaacaaac cccgatggtt tcgctggact ctgcaggggt cacttcctca
     2341 ccaagggcca gaacgcttgc aagacttggg atgccaccgc tgatggctac tgccgtgctg
     2401 agggtgtggg atctattgtc atcaagaggc tcgaagacgc agaggcggac aacgacaaca
     2461 ttcttggtgt cgtcctcgct gccggaacca accactctgc cgaagctgtc tccattactc
     2521 acccacacgc tggacaccaa tcttatctgt cccgtcagat cctccgacag gcaggagttg
     2581 atcctttgga tgtctcctac attgagcttc acggcactgg cacccaggca ggcgatttcg
     2641 aggagctgaa gggtgttctg gacgtctatg caccaaacac ctcccctagt cgtcgcactg
     2701 accaggttct ccacattgga agctccaagt ccaacgttgg ccacggcgag tcggtcgcag
     2761 gtacgaccgc actgatcaag gttcttttga tgcttcagaa gaacgccatc ccccgccaca
     2821 ttggtatcaa gactgagatc aacccgcgtc ttcccactga tcttgatcag aggaacgtcg
     2881 tcattccctt ttccaacacc ccatgggctc ctggaaagcg cattgccgct gtcaacaact
     2941 ttggagcagc tggtggcaac accatgatga tcttggaaga ggcccctgcc cgcgaagcac
     3001 gtcaagagaa agacccccgt cagactcatg ttgtgaccct ctcagccaag accaaggcct
     3061 cgctccgtgg caacatcgaa cgcctcctcg ctcatctcga gagggacgaa accgtggcat
     3121 tggaggatct tgcctacacc accactgcac gcaagtacca ccactcatac cggatagcca
     3181 tctccgcttc cgatattggt tccgtcaaga agcagcttac ttcgcagctg aacaagattg
     3241 atgctttgaa gcctgtcaac aagaccaacc ccccgtctgt ggctttcgcc ttcaccggtc
     3301 agggcgcatc ttaccagtcg atgaacctcg agctctaccg cgatgtcccc gtcttccgag
     3361 agcacatccg cacccttgac aagctggctc aggctcaagg cttcccgtcc ttcattcccg
     3421 ctatcgacgg ctcgcacccc aaggaatatg ttcatgctcc tgtaatcacc cagctagcac
     3481 tcgtctgtgt cgagattgcg ctcgcgcact actgggcttc cctcggcatc aagcctgacg
     3541 ttgttgttgg ccactctctc ggcgagtacg cagctatgca cgtcgctggt gtcatcagcg
     3601 ccaacgacgc catcttcatg gtcggttcca gggccctgat gctcatggag aagtgccagt
     3661 caggcagcca ctgcatgatg gctgtacgcg cctctctgga aaacatcatc gccaactcag
     3721 gcggcaagcc ccacactgtg gcctgcatca acggcccagc tgatactgtc ttgagcggca
     3781 caaaggccca gatggacgac atcgccaagg acctcgaggc tgctggactc cgatgcatca
     3841 agcttgatgt tgcctttgcc ttccactccg agcagactga ccccattctc gacgaatttg
     3901 aggccatcag caagagcggt gtcgtgttcc atgagcccaa gctgcccatc atctcgcccc
     3961 ttctcggcaa ggtcatcttc gatggccaca ctttgaacgc caactatgtg cgcaccgcca
     4021 cccgcgaggc tgtcgacttt gtctctgcca tgaggaacgc ttccagcatt tccaccatca
     4081 gcgacgacac cgtctggatt gagattggtc cccaccccgt ctgcgttgga ttcatcaaga
     4141 ccaccctgcc ttctacccgc cttgcagttc cttctctgcg ccgtggagac agcaactgga
     4201 agaccatgtc ggatagtgtt gcagctcttc accttgctgg tgtcaatgtc gactggaacg
     4261 agctccaccg tcctttctct ggccaggtcc gccttcttga gcttccgacc tatgcttggg
     4321 acgagaagaa ctactggatg cagtacaacg gtgactggtg cctgaccaag ggcaacactt
     4381 tctacgagaa gaagcagaag accatcgagc ccgccatcgg caacaccaag cagtaccagt
     4441 cattgggctc tttggttcag aacattatct cagttaatgt cgagggcgag gcgggtagtg
     4501 tggtcatgca gtccgacctc atgcagaagg agttcttgga ggcagcttca ggacatcgta
     4561 tgaacgacaa tgctgtcgtc acatcgtcta ttcatgcgga tatcgcttac accctgggtg
     4621 actacctcta ccgcaagttt taccccaagg ccaagcgccc ggatatggac atggccaacc
     4681 ttgaggtcac caaggctctc attgctaaga agaaaggcat ggatacacct cagtctatcc
     4741 aagtcactgc cagcaccacc aatatccgat ctggtgtcct ggacctgact tggcagaacg
     4801 tagaatctga tggcagctgc gctgagcctt ttgctacagc ccagatcatc tatggcgatg
     4861 ctaagcagtg gctctcatcc tggagcccca tgtcccatct tatccaaagc cgcatcgagt
     4921 cacttgagcg tctagctgtc gagggacaag ctaaccgtct atcccgcaac ttggcctaca
     4981 ctctattctc ctccaaccta gtcgactatg ccgacaagta ccgcggcatg caatccgtcg
     5041 tcatgaacga cctagaagcc tttgccgaag ttaagcttac cgagaagccc gacagcggca
     5101 cctacgccgt gccaccctac tacatcgaca gtgtagcgca tttggcggga ttcatcatga
     5161 actgctccga ctctattgac accaagaaca actattgcgt tacttctggc tggaagtcta
     5221 tgcgcttcgc cgagcccatg actccgggag ctagataccg atcttatgtc aagatgattc
     5281 ctaccaagga ggacccagct gtcttccttg gtgatgttta cattctcaag gcagaagaca
     5341 actctattgt tggaatggtt ggtggcatcc acttccgccg cttccctcgt atcctgctca
     5401 accgcttctt cagcgccccc gagcctgcta aagcgtcgtc cggtcagcaa gccaagccag
     5461 cacccgcaaa ggccgcgcct gcacccaagc ccgtcgcaca aaagaccatc gctgctgccc
     5521 ccaaggcaca ggactttgac gctcaatcta gcagcagctc ggaggtgact cctcctggca
     5581 ccccgctgct tgctgcctcg gccaacactt cttccactag catcaacgtc attgctgccg
     5641 agccagcgaa gaagggcggc aacggtatca tggacaaagc gcttgacatc atcgccaacg
     5701 agacagctat tgacctggcc gacctggaag atgacgccga ctttgccaat cttggtattg
     5761 atagtttgat gtcgcttgtg ctagtagaga agttccagaa ggagcttgat gtcaaggtta
     5821 acggcagttt gtttttggat tatcctacca ttggcgattt gcgcgagtgg ttggagaagt
     5881 actatggtta gggagattga taagactttt ctagttgcat atcttttata tttaaatctt
     5941 atctattagt taattttttg taatttatcc ttatatatag tctggttatt ctaaaatatc
     6001 atttcagtat ctaaaaattc ccctcttttt tcagttatat cttaacaggc gacagtccaa
     6061 atgttgattt atcccagtcc gattcatcag ggttgtgaag cattttgtca atggtcgaaa
     6121 tcacatcagt aatagtgcct cttacttgcc tcatagaatt tctttctctt aacgtcaccg
     6181 tttggtcttt tatagtttcg aaatctatgg tgataccaaa tggtgttccc aattcatcgt
     6241 tacgggcgta ttttttacca attgaagtat tggaatcgtc aattttaaag tatatctctc
     6301 ttttacgtaa agcctgcgag atcctcttaa gtatagcggg gaagccatcg ttattcgata
     6361 ttgtcgtaac aaatactttg atcggcgcta tgcggccgcc accgcggtgg agctccagct
     6421 tttgttccct ttagtgaggg ttaattgcgc gcttggcgag actcaaacaa gatatatttt
     6481 gcccgcctta cagacgaaac tagttggagg ttatggagca tactatcacg tgggcggcca
     6541 ctggtgagtt actacacccc aggggcaacg ttgatgctcc taaaaaactc tggctggacg
     6601 caagccgtaa cacccgtgtc acttcataat cgtttgcaat tcagggcttg atctacacat
     6661 taccctgtta tccctattaa ttaatacggt tatccacaga atcaggggat aacgcaggaa
     6721 agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg
     6781 cgtttttcca taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga
     6841 ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg
     6901 tgcgctctcc tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg
     6961 gaagcgtggc gctttctcat agctcacgct gtaggtatct cagttcggtg taggtcgttc
     7021 gctccaagct gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg
     7081 gtaactatcg tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca
     7141 ctggtaacag gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt
     7201 ggcctaacta cggctacact agaaggacag tatttggtat ctgcgctctg ctgaagccag
     7261 ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg
     7321 gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc
     7381 ctttgatctt ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt
     7441 tggtcatgag attatcaaaa aggatcttca cctagatcct tttaaattaa aaatgaagtt
     7501 ttaaatcaat ctaaagtata tatgagtaaa cttggtctga cagttaccaa tgcttaatca
     7561 gtgaggcacc tatctcagcg atctgtctat ttcgttcatc catagttgcc tgactccccg
     7621 tcgtgtagat aactacgata cgggagggct taccatctgg ccccagtgct gcaatgatac
     7681 cgcgagaccc acgctcaccg gctccagatt tatcagcaat aaaccagcca gccggaaggg
     7741 ccgagcgcag aagtggtcct gcaactttat ccgcctccat ccagtctatt aattgttgcc
     7801 gggaagctag agtaagtagt tcgccagtta atagtttgcg caacgttgtt gccattgcta
     7861 caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc ggttcccaac
     7921 gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc
     7981 ctccgatcgt tgtcagaagt aagttggccg cagtgttatc actcatggtt atggcagcac
     8041 tgcataattc tcttactgtc atgccatccg taagatgctt ttctgtgact ggtgagtact
     8101 caaccaagtc attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa
     8161 tacgggataa taccgcgcca catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt
     8221 cttcggggcg aaaactctca aggatcttac cgctgttgag atccagttcg atgtaaccca
     8281 ctcgtgcacc caactgatct tcagcatctt ttactttcac cagcgtttct gggtgagcaa
     8341 aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa tgttgaatac
     8401 tcatactctt cctttttcaa tattattgaa gcatttatca gggttattgt ctcatgagcg
     8461 gatacatatt tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc acatttcccc
     8521 gaaaagtgcc acctgaacga agcatctgtg cttcattttg tagaacaaaa atgcaacgcg
     8581 agagcgctaa tttttcaaac aaagaatctg agctgcattt ttacagaaca gaaatgcaac
     8641 gcgaaagcgc tattttacca acgaagaatc tgtgcttcat ttttgtaaaa caaaaatgca
     8701 acgcgagagc gctaattttt caaacaaaga atctgagctg catttttaca gaacagaaat
     8761 gcaacgcgag agcgctattt taccaacaaa gaatctatac ttcttttttg ttctacaaaa
     8821 atgcatcccg agagcgctat ttttctaaca aagcatctta gattactttt tttctccttt
     8881 gtgcgctcta taatgcagtc tcttgataac tttttgcact gtaggtccgt taaggttaga
     8941 agaaggctac tttggtgtct attttctctt ccataaaaaa agcctgactc cacttcccgc
     9001 gtttactgat tactagcgaa gctgcgggtg cattttttca agataaaggc atccccgatt
     9061 atattctata ccgatgtgga ttgcgcatac tttgtgaaca gaaagtgata gcgttgatga
     9121 ttcttcattg gtcagaaaat tatgaacggt ttcttctatt ttgtctctat atactacgta
     9181 taggaaatgt ttacattttc gtattgtttt cgattcactc tatgaatagt tcttactaca
     9241 atttttttgt ctaaagagta atactagaga taaacataaa aaatgtagag gtcgagttta
     9301 gatgcaagtt caaggagcga aaggtggatg ggtaggttat atagggatat agcacagaga
     9361 tatatagcaa agagatactt ttgagcaatg tttgtggaag cggtattcgc aatattttag
     9421 tagctcgtta cagtccggtg cgtttttggt tttttgaaag tgcgtcttca gagcgctttt
     9481 ggttttcaaa agcgctctga agttcctata ctttctagag aataggaact tcggaatagg
     9541 aacttcaaag cgtttccgaa aacgagcgct tccgaaaatg caacgcgagc tgcgcacata
     9601 cagctcactg ttcacgtcgc acctatatct gcgtgttgcc tgtatatata tatacatgag
     9661 aagaacggca tagtgcgtgt ttatgcttaa atgcgtactt atatgcgtct atttatgtag
     9721 gatgaaaggt agtctagtac ctcctgtgat attatcccat tccatgcggg gtatcgtatg
     9781 cttccttcag cactaccctt tagctgttct atatgctgcc actcctcaat tggattagtc
     9841 tcatccttca atgctatcat ttcctttgat attggatcat actaagaaac cattattatc
     9901 atgacattaa cctataaaaa taggcgtatc acgaggccct ttcgtctcgc gcgtttcggt
     9961 gatgacggtg aaaacctcgg cgcgccatta ccctgttatc cctaatatac aacgcaaaga
    10021 agctggaaaa ttggcgaggg aatcctgttt ctgtctatcc aagaatgggc atgaggtggc
    10081 aaccgtcgtg ctagcgtaca gggtgcactt tgtaaccatt tgggacaccg gacactcgct
    10141 gttttcgaaa ttacccttta agcgcgggta ttgaaccagg cttatgccca agatcgtagc
    10201 aagcatttaa ctgtgggaat actcaggtat cgtaagatgc aagagttcga atctcttagc
    10261 aaccattatt tttttcctca acataacgag aacacacagg ggcgctatcg cacagaatca
    10321 aattcgatga ctggaaattt tttgttaatt tcagaggtcg cctgacgcat ataccttttt
    10381 caactgaaaa attgggagaa aaaggaaagg tgagaggccg gaaccggctt ttcatataga
    10441 atagagaagc gttcatgact aaatgcttgc atcacaatac ttgaagttga caatattatt
    10501 taaggaccta ttgttttttc caataggtgg ttagcaatcg tcttactttc taacttttct
    10561 taccttttac atttcagcaa tatatatata tatttcaagg atataccatt ctaatgtctg
    10621 cccctatgtc tgcccctaag aagatcgtcg ttttgccagg tgaccacgtt ggtcaagaaa
    10681 tcacagccga agccattaag gttcttaaag ctatttctga tgttcgttcc aatgtcaagt
    10741 tcgatttcga aaatcattta attggtggtg ctgctatcga tgctacaggt gtcccacttc
    10801 cagatgaggc gctggaagcc tccaagaagg ttgatgccgt tttgttaggt gctgtggctg
    10861 gtcctaaatg gggtaccggt agtgttagac ctgaacaagg tttactaaaa atccgtaaag
    10921 aacttcaatt gtacgccaac ttaagaccat gtaactttgc atccgactct cttttagact
    10981 tatctccaat caagccacaa tttgctaaag gtactgactt cgttgttgtc agagaattag
    11041 tgggaggtat ttactttggt aagagaaagg aagacgatgg tgatggtgtc gcttgggata
    11101 gtgaacaata caccgttcca gaagtgcaaa gaatcacaag aatggccgct ttcatggccc
    11161 tacaacatga gccaccattg cctatttggt ccttggataa agctaatctt ttggcctctt
    11221 caagattatg gagaaaaact gtggaggaaa ccatcaagaa cgaattccct acattgaagg
    11281 ttcaacatca attgattgat tctgccgcca tgatcctagt taagaaccca acccacctaa
    11341 atggtattat aatcaccagc aacatgtttg gtgatatcat ctccgatgaa gcctccgtta
    11401 tcccaggttc cttgggtttg ttgccatctg cgtccttggc ctctttgcca gacaagaaca
    11461 ccgcatttgg tttgtacgaa ccatgccacg gttctgctcc agatttgcca aagaataagg
    11521 ttgaccctat cgccactatc ttgtctgctg caatgatgtt gaaattgtca ttgaacttgc
    11581 ctgaagaagg taaggccatt gaagatgcag ttaaaaaggt tttggatgca ggtatcagaa
    11641 ctggtgattt aggtggttcc aacagtacca ccgaagtcgg tgatgctgtc gccgaagaag
    11701 ttaagaaaat ccttgcttaa aaagattctc tttttttatg atatttgtac ataaacttta
    11761 taaatgaaat tcataataga aacgacacga aattacaaaa tggaatatgt tcatagggta
    11821 gacgaaacta tatacgcaat ctacatacat ttatcaagaa ggagaaaaag gaggatagta
    11881 aaggaataca ggtaagcaaa ttgatactaa tggctcaacg aaatgacggt atcgataagc
    11941 ttgatatcga attcctgcag cccgggggat ccactagttc tagagttaat taa
//
