LOCUS       sequence-208203-       10617 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of a fungal biosynthetic gene cluster.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10617)
  AUTHORS   Harvey CJB, Tang M, Schlecht U, Horecka J, Fischer CR, Lin HC, Li J,
            Naughton B, Cherry J, Miranda M, Li YF, Chu AM, Hennessy JR, Vandova
            GA, Inglis D, Aiyar RS, Steinmetz LM, Davis RW, Medema MH, Sattely 
            E, Khosla C, St Onge RP, Tang Y, Hillenmeyer ME
  TITLE     HEx: A heterologous expression platform for the discovery of fungal 
            natural products.
  JOURNAL   Sci Adv. 2018 Apr 11;4(4):eaar5459. doi: 10.1126/sciadv.aar5459. 
            eCollection 2018 Apr.
  PUBMED    29651464
REFERENCE   2  (bases 1 to 10617)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Adv."; 
            date: "2018-04-11"; volume: "4(4):eaar5459. doi"; pages: " 
            10.1126/sciadv.aar5459. eCollection 2018 Apr"
COMMENT     Sequence Label: pCHSU65.2-2.2
FEATURES             Location/Qualifiers
     source          1..10617
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      1512..1811
                     /label=ADH2 terminator
                     /note="transcription terminator for the S. cerevisiae 
                     alcohol dehydrogenase 2 (ADH2) gene"
     promoter        3649..3869
                     /gene="S. cerevisiae URA3"
                     /label=URA3 promoter
     CDS             3870..4673
                     /codon_start=1
                     /gene="S. cerevisiae URA3"
                     /product="orotidine-5'-phosphate decarboxylase, required 
                     for uracil biosynthesis"
                     /label=URA3
                     /note="yeast auxotrophic marker, counterselectable with 
                     5-fluoroorotic acid (5-FOA)"
                     /translation="MSKATYKERAATHPSPVAAKLFNIMHEKQTNLCASLDVRTTKELL
                     ELVEALGPKICLLKTHVDILTDFSMEGTVKPLKALSAKYNFLLFEDRKFADIGNTVKLQ
                     YSAGVYRIAEWADITNAHGVVGPGIVSGLKQAAEEVTKEPRGLLMLAELSCKGSLSTGE
                     YTKGTVDIAKSDKDFVIGFIAQRDMGGRDEGYDWLIMTPGVGLDDKGDALGQQYRTVDD
                     VVSTGSDIIIVGRGLFAKGRDAKVEGERYRKAGWEAYLRRCGQQN"
     primer_bind     complement(6764..6784)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(6764..6782)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     rep_origin      complement(7121..7709)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7201..7220)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7880..8671,8672..8740))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8503..8522
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8741..8845)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      8872..10214
                     /label=2u ori
                     /note="yeast 2u plasmid origin of replication"
     protein_bind    complement(9835..9882)
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     primer_bind     10255..10273
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(10592..10608)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(10592..10608)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
ORIGIN
        1 tatctaaaaa ttgccttatg atccgtctct ccggttacag cctgtgtaac tgattaatcc
       61 tgcctttcta atcaccattc taatgtttta attaagggat tttgtcttca ttaacggctt
      121 tcgctcataa aaatgttatg acgttttgcc cgcaggcggg aaaccatcca cttcacgaga
      181 ctgatctcct ctgccggaac accgggcatc tccaacttat aagttggaga aataagagaa
      241 tttcagattg agagaatgaa aaaaaaaaaa aaaaaaaagg cagaggagag catagaaatg
      301 gggttcactt tttggtaaag ctatagcatg cctatcacat ataaatagag tgccagtagc
      361 gacttttttc acactcgaaa tactcttact actgctctct tgttgttttt atcacttctt
      421 gtttcttctt ggtaaataga atatcaagct acaaaaagca tacaatcaac tatcaactat
      481 taactatatc gtaatacaca atgtcgatga catcaggcaa caccaaggat aagggcggct
      541 acagacaaat caacaagtcc ctcaacatct gcgcctttga agattacctc aacggccaaa
      601 cagcagggtt gaagcctatc ccagatgtcg aacaagtcac accaagagtg atgcgtgtac
      661 gaggacagaa ccccggcaag ttcacaatgc aaggcacaaa cacgttccta gtcggcactg
      721 gcgcttctcg catcctcatc gacacctcag gcggcgagcc agaatacgca aagctactcg
      781 catccactct ggaattccta aacatcagca tcaaatacgt cctcctcaca cactggcacg
      841 gcgaccacac gggcggagtc ccagacctac tccgcctcta cccccaccta gaaaaccaca
      901 tctacaagaa cataccagag gtcggccacc gcgacatctc agacggtcaa ctgttccacg
      961 tcgagggcgc aacactcgtg gctgtacaca caccgggcca cgccgaagat cacatgtgct
     1021 ttgtgctcaa ggaagaaaac tcaatgttca ctggtgacaa catcctcggt acgggtacca
     1081 gcgcagtcga ggacttgggt atcttcatgt ccagtctgca aaagatgttg ggaaagaact
     1141 gccaaaccgg acaccccgcc cacggccaaa ccatcggtga cctaaacgca aagatatcaa
     1201 aggaactcat gtcaaagttc cgacgagaga agcaggtact gcaggccatg cgcgacctac
     1261 gggatgctga tgagaggcgg ccaaacgttc gaatgattgt cgatcgcatg tatggaagct
     1321 ccgtcaacga gactacgcgg acattggcct tggagccgtt tattggcgag gtactgcgga
     1381 aacttgctgg tgatggcaag gttgggtttg gcgtgcgggc tgggaggaag ggctggttcc
     1441 tggttccaaa tgagcagcag agtacgcagt tacggcatac acattcggtt tttgtaagag
     1501 ctagtgcata ggcggatctc ttatgtcttt acgatttata gttttcatta tcaagtatgc
     1561 ctatattagt atatagcatc tttagatgac agtgttcgaa gtttcacgaa taaaagataa
     1621 tattctactt tttgctccca ccgcgtttgc tagcacgagt gaacaccatc cctcgcctgt
     1681 gagttgtacc cattcctcta aactgtagac atggtagctt cagcagtgtt cgttatgtac
     1741 ggcatcctcc aacaaacagt cggttatagt ttgtcctgct cctctgaatc gtctccctcg
     1801 atatttctca ttttccttcg catgccagca ttgaaatgat cgaagttcaa tgatgaaacg
     1861 gtaattcttc tgtcatttac tcatctcatc tcatcaagtt atataattct atacggatgt
     1921 aatttttcac ttttcgtctt gacgtccacc ctataatttc aattattgaa ccctcacgat
     1981 ccagttctcc agtgacacag cctttatctg gtcaaacctt tctttctaat cacctatgct
     2041 gatgcttaat taagggattt ttgtctccat caacggcatg cgcccaaaaa tgacgttttt
     2101 tttaacccat agacacgaaa ctacccattt tccaccggcc tgacctacca ccggaacaac
     2161 ggccatctcc aacttgcaag ttggggaaat taagagcatc gcaggtttaa tggaagaaaa
     2221 aaaaaaggta cagcacagcg caaatggagt tagttccctt atgtcacaca ctcacacaca
     2281 gtcggtcaga tcaagcatac tgggtgcgta taaatagagt ggccattgcc accctgttta
     2341 tctcaaaatc tgtcttgtta gtggtcttct ccctttttca ggttacaatt ctcttgtttc
     2401 tacttagtat ataagtatat caagctatat taagcatact atcaactgtc aactctatcc
     2461 tcaaaataca atacaaaatg gataacacga ccctcttcga cgtcaagaac aaaattgtcc
     2521 tcgtgaccgg cggcgccaaa ggcatcggcc gcatgataag cgaaggtttc atccaaaacg
     2581 gcgcaaacgt ctacatctcg tcccgcgacg cgaaagcgtg cgagcaagca tgtaaagagc
     2641 tgaatgcact gggaaaaggc aaagcagagt ttatcgttgc ggatttgtat cgggaggatg
     2701 cggcgagttt tgttgcgggg gagttgcaga agagagtggg aaaactcgac gtcctagtga
     2761 acaacagtgg gagtaactgg ggcgagtcat acgacacata cccatcgtcc gcctgggacc
     2821 gcgttctaac cctaaaccta aaacgcgttt tccaactcac tcaagccgtt acaccccttc
     2881 tctctgctgc cgcctccccc tcgtcccccg cacgcatcat caacattggc agcatagacg
     2941 gtctccgtgt cccagctctc gaaacgtttg cctattcagc ctctaaagcg ggtctgcatc
     3001 acatgagcaa agtactagcg aaccatttgg gaaagaggaa tatcacggtg aataccgtgg
     3061 cgtgtgggcc gtttgagagt aagatgatga aggcgacgct ggagaagcat aggggcgtta
     3121 ttgaggaggg gattccgctg gggaggattg gagggaagga ggatgtagcg ggtacgtgta
     3181 tttggttggc gagtcgcgct ggggcgtggg ttaatgggat tgtggtggca ttggatgggg
     3241 ggagtgttat tggagctaag ctttgaacaa atcgctctta aatatatacc taaagaacat
     3301 taaagctata ttataagcaa agatacgtaa attttgctta tattattata cacatatcat
     3361 atttctatat ttttaagatt tggttatata atgtacgtaa tgcaaaggaa ataaatttta
     3421 tacattattg aacagcgtcc aagtaactac attatgtgca ctaatagttt agcgtcgtga
     3481 agactttatt gtgtcgcgaa aagtaaaaat tttaaaaatt agagcacctt gaacttgcga
     3541 aaaaggttct catcaactgt ttaaaaggag gatatcaggt cctatttctg acaaacaata
     3601 tacaaattta gtttcaaaga tgaatcagtg cgcgaaggac ataactcatt caattcaatt
     3661 catcattttt tttttattct tttttttgat ttcggtttct ttgaaatttt tttgattcgg
     3721 taatctccga acagaaggaa gaacgaagga aggagcacag acttagattg gtatatatac
     3781 gcatatgtag tgttgaagaa acatgaaatt gcccagtatt cttaacccaa ctgcacagaa
     3841 caaaaacctg caggaaacga agataaatca tgtcgaaagc tacatataag gaacgtgctg
     3901 ctactcatcc tagtcctgtt gctgccaagc tatttaatat catgcacgaa aagcaaacaa
     3961 acttgtgtgc ttcattggat gttcgtacca ccaaggaatt actggagtta gttgaagcat
     4021 taggtcccaa aatttgttta ctaaaaacac atgtggatat cttgactgat ttttccatgg
     4081 agggcacagt taagccgcta aaggcattat ccgccaagta caatttttta ctcttcgaag
     4141 acagaaaatt tgctgacatt ggtaatacag tcaaattgca gtactctgcg ggtgtataca
     4201 gaatagcaga atgggcagac attacgaatg cacacggtgt ggtgggccca ggtattgtta
     4261 gcggtttgaa gcaggcggca gaagaagtaa caaaggaacc tagaggcctt ttgatgttag
     4321 cagaattgtc atgcaagggc tccctatcta ctggagaata tactaagggt actgttgaca
     4381 ttgcgaagag cgacaaagat tttgttatcg gctttattgc tcaaagagac atgggtggaa
     4441 gagatgaagg ttacgattgg ttgattatga cacccggtgt gggtttagat gacaagggag
     4501 acgcattggg tcaacagtat agaaccgtgg atgatgtggt ctctacagga tctgacatta
     4561 ttattgttgg aagaggacta tttgcaaagg gaagggatgc taaggtagag ggtgaacgtt
     4621 acagaaaagc aggctgggaa gcatatttga gaagatgcgg ccagcaaaac taaaaaactg
     4681 tattataagt aaatgcatgt atactaaact cacaaattag agcttcaatt taattatatc
     4741 agttattacc ctatgcggtg tgaaataccg cacagatgcg taaggagaaa ataccgcatc
     4801 aggataggaa aaaaccgagc ttcctttcat ccggcgcggc tgtgttctac atatcactga
     4861 agctccgggt attttaagtt atacaaggga aagatgccgg ctagactagc aagttttagg
     4921 ctgcttaaca ttatggatag gcggataaag ggcccaaaca ggattgtaaa gcttagacgc
     4981 ttctggttgg acaatggtac gtttgtgtat taagtaaggc ttggctgggg atagcaacat
     5041 tgggcagagt atagaagacc acaaaaaaaa ggtatataag ggcagagaag tctttgtaat
     5101 gtgtgtaact tctcttccat gtgtaatcag tatttctact tacttcttaa atatacagaa
     5161 gtaagacaga taaccaacag cctttcccag atatacatat atatctttat ttcagcttaa
     5221 acaataatta tatttgttta actcaaaaat aaaaaaaaaa aaccaaactc acgcaactaa
     5281 ttattccata ataaaataac aacatggaga gcccttttgt gatgccgcag gaggatgtgg
     5341 aggcgctggg tcagtcaaag gcgttttgcg cgctgaggag gtgtcttgct ggtgaggccg
     5401 cgttttgcgc aacacacgcg cattgcacac cccaagccaa acaaacatgg ctccttcacc
     5461 gcgacattct ccacgccctc atcatgcctg tagtgacgct cttttcgcgc gcctcaactc
     5521 tggcgtcggc cgccctctgt acgcagcgaa cgtcggacct cgaacttgcg ttttcgggtg
     5581 aatcgcgcag cgctttcata ggcctgcaat gcttcatcag tgaggaggct gactggtgcc
     5641 gcacgagagg gtgtccggct tgcgtcgtct cggccacgtt gagcaccgac agccatatcc
     5701 gcctgacaat tgctgcgagc ctgctcagca ctgcgagtgt agccacgccc acgcaagagg
     5761 aacctcgaca cgacagtgat aggacgctgc ctccactccc gcatatcctt cctgccctgc
     5821 aacacgccgt gataaacgac ccgttctggg aaggctcgga catctggggc tacatcttgt
     5881 cgcgtgccac ccaactgtcg gctggcatac aagccctaat cgccgagtgt gtgaatcttg
     5941 aatcgctcgt ctcatcgccc acaacagaca ggccgggtgc gaagcgcggc ttgacgcatc
     6001 cctcggtgcc ttttggagct tctggccagg ccgtcgagga aaagggcgtc aaactcagaa
     6061 agagcaagct agcaaagcgg cagttgaggc tacgagacga ggaggttgag ctgatgagga
     6121 gagtagccat gcaatgctgg gccaaagccc aggtaccaaa gaaaatcaga ggcgatgtct
     6181 tgggcataag cgatggcaag aggacgagga gcttgacttg cccatgagga gattgataag
     6241 acttttctag ttgcatatct tttatattta aatcttatct attagttaat tttttgtaat
     6301 ttatccttat atatagtctg gttattctaa aatatcattt cagtatctaa aaattcccct
     6361 cttttttcag ttatatctta acaggcgaca gtccaaatgt tgatttatcc cagtccgatt
     6421 catcagggtt gtgaagcatt ttgtcaatgg tcgaaatcac atcagtaata gtgcctctta
     6481 cttgcctcat agaatttctt tctcttaacg tcaccgtttg gtcttttata gtttcgaaat
     6541 ctatggtgat accaaatggt gttcccaatt catcgttacg ggcgtatttt ttaccaattg
     6601 aagtattgga atcgtcaatt ttaaagtata tctctctttt acgtaaagcc tgcgagatcc
     6661 tcttaagtat agcggggaag ccatcgttat tcgatattgt cgtaacaaat actttgatcg
     6721 gcgctatgcg gccgccaccg cggtggagct ccagcttttg ttccctttag tgagggttaa
     6781 ttgcgcgctt ggcgagactc aaacaagata tattttgccc gccttacaga cgaaactagt
     6841 tggaggttat ggagcatact atcacgtggg cggccactgg tgagttacta caccccaggg
     6901 gcaacgttga tgctcctaaa aaactctggc tggacgcaag ccgtaacacc cgtgtcactt
     6961 cataatcgtt tgcaattcag ggcttgatct acacattacc ctgttatccc tattaattaa
     7021 tacggttatc cacagaatca ggggataacg caggaaagaa catgtgagca aaaggccagc
     7081 aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg ctccgccccc
     7141 ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat
     7201 aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc
     7261 cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct
     7321 cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg
     7381 aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc
     7441 cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga
     7501 ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa
     7561 ggacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta
     7621 gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc
     7681 agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg
     7741 acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgagatta tcaaaaagga
     7801 tcttcaccta gatcctttta aattaaaaat gaagttttaa atcaatctaa agtatatatg
     7861 agtaaacttg gtctgacagt taccaatgct taatcagtga ggcacctatc tcagcgatct
     7921 gtctatttcg ttcatccata gttgcctgac tccccgtcgt gtagataact acgatacggg
     7981 agggcttacc atctggcccc agtgctgcaa tgataccgcg agacccacgc tcaccggctc
     8041 cagatttatc agcaataaac cagccagccg gaagggccga gcgcagaagt ggtcctgcaa
     8101 ctttatccgc ctccatccag tctattaatt gttgccggga agctagagta agtagttcgc
     8161 cagttaatag tttgcgcaac gttgttgcca ttgctacagg catcgtggtg tcacgctcgt
     8221 cgtttggtat ggcttcattc agctccggtt cccaacgatc aaggcgagtt acatgatccc
     8281 ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc agaagtaagt
     8341 tggccgcagt gttatcactc atggttatgg cagcactgca taattctctt actgtcatgc
     8401 catccgtaag atgcttttct gtgactggtg agtactcaac caagtcattc tgagaatagt
     8461 gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg ggataatacc gcgccacata
     8521 gcagaacttt aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa ctctcaagga
     8581 tcttaccgct gttgagatcc agttcgatgt aacccactcg tgcacccaac tgatcttcag
     8641 catcttttac tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa
     8701 aaaagggaat aagggcgaca cggaaatgtt gaatactcat actcttcctt tttcaatatt
     8761 attgaagcat ttatcagggt tattgtctca tgagcggata catatttgaa tgtatttaga
     8821 aaaataaaca aataggggtt ccgcgcacat ttccccgaaa agtgccacct gaacgaagca
     8881 tctgtgcttc attttgtaga acaaaaatgc aacgcgagag cgctaatttt tcaaacaaag
     8941 aatctgagct gcatttttac agaacagaaa tgcaacgcga aagcgctatt ttaccaacga
     9001 agaatctgtg cttcattttt gtaaaacaaa aatgcaacgc gagagcgcta atttttcaaa
     9061 caaagaatct gagctgcatt tttacagaac agaaatgcaa cgcgagagcg ctattttacc
     9121 aacaaagaat ctatacttct tttttgttct acaaaaatgc atcccgagag cgctattttt
     9181 ctaacaaagc atcttagatt actttttttc tcctttgtgc gctctataat gcagtctctt
     9241 gataactttt tgcactgtag gtccgttaag gttagaagaa ggctactttg gtgtctattt
     9301 tctcttccat aaaaaaagcc tgactccact tcccgcgttt actgattact agcgaagctg
     9361 cgggtgcatt ttttcaagat aaaggcatcc ccgattatat tctataccga tgtggattgc
     9421 gcatactttg tgaacagaaa gtgatagcgt tgatgattct tcattggtca gaaaattatg
     9481 aacggtttct tctattttgt ctctatatac tacgtatagg aaatgtttac attttcgtat
     9541 tgttttcgat tcactctatg aatagttctt actacaattt ttttgtctaa agagtaatac
     9601 tagagataaa cataaaaaat gtagaggtcg agtttagatg caagttcaag gagcgaaagg
     9661 tggatgggta ggttatatag ggatatagca cagagatata tagcaaagag atacttttga
     9721 gcaatgtttg tggaagcggt attcgcaata ttttagtagc tcgttacagt ccggtgcgtt
     9781 tttggttttt tgaaagtgcg tcttcagagc gcttttggtt ttcaaaagcg ctctgaagtt
     9841 cctatacttt ctagagaata ggaacttcgg aataggaact tcaaagcgtt tccgaaaacg
     9901 agcgcttccg aaaatgcaac gcgagctgcg cacatacagc tcactgttca cgtcgcacct
     9961 atatctgcgt gttgcctgta tatatatata catgagaaga acggcatagt gcgtgtttat
    10021 gcttaaatgc gtacttatat gcgtctattt atgtaggatg aaaggtagtc tagtacctcc
    10081 tgtgatatta tcccattcca tgcggggtat cgtatgcttc cttcagcact accctttagc
    10141 tgttctatat gctgccactc ctcaattgga ttagtctcat ccttcaatgc tatcatttcc
    10201 tttgatattg gatcatacta agaaaccatt attatcatga cattaaccta taaaaatagg
    10261 cgtatcacga ggccctttcg tctcgcgcgt ttcggtgatg acggtgaaaa cctcggcgcg
    10321 ccattaccct gttatcccta atatacaacg caaagaagct ggaaaattgg cgagggaatc
    10381 ctgtttctgt ctatccaaga atgggcatga ggtggcaacc gtcgtgctag cgtacagggt
    10441 gcactttgta accatttggg acaccggaca ctcgctgttt tcgaaattac cctttaagcg
    10501 cgggtattga accaggctta tgcccaagat cgtagcaagc atttaaatga cggtatcgat
    10561 aagcttgata tcgaattcct gcagcccggg ggatccacta gttctagagt taattaa
//
