LOCUS       sequence-208204-       12011 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of a fungal biosynthetic gene cluster.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12011)
  AUTHORS   Harvey CJB, Tang M, Schlecht U, Horecka J, Fischer CR, Lin HC, Li J,
            Naughton B, Cherry J, Miranda M, Li YF, Chu AM, Hennessy JR, Vandova
            GA, Inglis D, Aiyar RS, Steinmetz LM, Davis RW, Medema MH, Sattely 
            E, Khosla C, St Onge RP, Tang Y, Hillenmeyer ME
  TITLE     HEx: A heterologous expression platform for the discovery of fungal 
            natural products.
  JOURNAL   Sci Adv. 2018 Apr 11;4(4):eaar5459. doi: 10.1126/sciadv.aar5459. 
            eCollection 2018 Apr.
  PUBMED    29651464
REFERENCE   2  (bases 1 to 12011)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Adv."; 
            date: "2018-04-11"; volume: "4(4):eaar5459. doi"; pages: " 
            10.1126/sciadv.aar5459. eCollection 2018 Apr"
COMMENT     Sequence Label: pCHSU67.1-2.2
FEATURES             Location/Qualifiers
     source          1..12011
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             504..527
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     primer_bind     complement(6446..6466)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(6446..6464)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     rep_origin      complement(6803..7391)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6883..6902)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7562..8353,8354..8422))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8185..8204
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8423..8527)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      8554..9896
                     /label=2u ori
                     /note="yeast 2u plasmid origin of replication"
     protein_bind    complement(9517..9564)
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     primer_bind     9937..9955
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        10227..10631
                     /gene="S. cerevisiae LEU2"
                     /label=LEU2 promoter
     CDS             10644..11738
                     /codon_start=1
                     /gene="S. cerevisiae LEU2"
                     /product="3-isopropylmalate dehydrogenase, required for 
                     leucine biosynthesis"
                     /label=LEU2
                     /note="yeast auxotrophic marker"
                     /translation="MSAPKKIVVLPGDHVGQEITAEAIKVLKAISDVRSNVKFDFENHL
                     IGGAAIDATGVPLPDEALEASKKVDAVLLGAVAGPKWGTGSVRPEQGLLKIRKELQLYA
                     NLRPCNFASDSLLDLSPIKPQFAKGTDFVVVRELVGGIYFGKRKEDDGDGVAWDSEQYT
                     VPEVQRITRMAAFMALQHEPPLPIWSLDKANLLASSRLWRKTVEETIKNEFPTLKVQHQ
                     LIDSAAMILVKNPTHLNGIIITSNMFGDIISDEASVIPGSLGLLPSASLASLPDKNTAF
                     GLYEPCHGSAPDLPKNKVDPIATILSAAMMLKLSLNLPEEGKAIEDAVKKVLDAGIRTG
                     DLGGSNSTTEVGDAVAEEVKKILA"
     primer_bind     complement(11986..12002)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(11986..12002)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
ORIGIN
        1 tatctaaaaa ttgccttatg atccgtctct ccggttacag cctgtgtaac tgattaatcc
       61 tgcctttcta atcaccattc taatgtttta attaagggat tttgtcttca ttaacggctt
      121 tcgctcataa aaatgttatg acgttttgcc cgcaggcggg aaaccatcca cttcacgaga
      181 ctgatctcct ctgccggaac accgggcatc tccaacttat aagttggaga aataagagaa
      241 tttcagattg agagaatgaa aaaaaaaaaa aaaaaaaagg cagaggagag catagaaatg
      301 gggttcactt tttggtaaag ctatagcatg cctatcacat ataaatagag tgccagtagc
      361 gacttttttc acactcgaaa tactcttact actgctctct tgttgttttt atcacttctt
      421 gtttcttctt ggtaaataga atatcaagct acaaaaagca tacaatcaac tatcaactat
      481 taactatatc gtaatacaca atggattata aggatgatga tgataagact agtatgggaa
      541 acatgaagct cctctatttc agcaatgagt ttccgaagga ggatctccag tacctctttc
      601 gttgcctcca caaccacagc aaggacagac gacataccat tctcgctcag tttctccatg
      661 aggctactct agccgtcaag gatgaaatcc gacacctctc aacagagctg aaacagttat
      721 tcccaccgtt cgaagatatt cttacctggg ctgagaatcc cgagctgcgc gaagggctcc
      781 tttgcggtgc tgtcgacgga gttctgctca cagtcgtcca gcttgcaaca tacattggtt
      841 atgtggagaa tcagccggaa gagctggcga gattagaaca gacatcactg gcgggcctag
      901 gcatcggctt actggcactg actgccgtgt cgctctcctc cgccgtggtg gatttgccgc
      961 tggctggtgc agatgcggtg cgtctcgctt tccgtatggg ctgccatgtt ctcgccgttt
     1021 ccgagaactt ggaatctcgc gatctcgcag cagctccaga gacatgggcc tacgtgattc
     1081 acaacgtcga tcctgtcgcg gcgcagcagg cgctcgatgc aatccaatcg cgagaaaaga
     1141 tacctgacac cagcaagata ttcctcagtg ctgtcagccg aacgtcagtc acagtcagtg
     1201 gaccaccctc gagattaaag gcgctcttca gtcgctcaga attcttccgg gactccagat
     1261 ctattgctct gcccgtctat ggaggacttt gccacgcgcc tcatgtctac ggctctcggg
     1321 acgttcaaag cattgtctgg ggtagttcgt tgaacgcaaa cctcgcaaaa tcctgtccca
     1381 tcaccccaat ttattccacc agtaccggtc tgccattcag tgcctcgaat gcgggagaat
     1441 tattcgaatg tgttatctcc gagttactca cgcatgcgat ctgctgggac caagtcatcc
     1501 ggggcgtggt tgctcaactc caaagtgctg gtgccagcca ggtagcgctt tcctccttcg
     1561 gtaactcaat ccccctcaac gacctcaaag catccttgga atccagcctt cctcaactcg
     1621 aagcatccat catcaatcta gtaccatgga ttttcgacgc tgcatttccc gcggcgtcac
     1681 cgcgaggctc ggcgcaatcc aagctcgcca ttgtgggcat gtcatgcagg ctcccgggcg
     1741 gagctacgaa cacagagaag ttctgggaaa tcctcgaaca ggggctcgac gtctctagac
     1801 gaatcccggc agatcgcttc gacattgaca cgcactatga cccgactggc aagcaaatga
     1861 acaagagcat gacgcagtac ggatgcttca tcgatgagcc cggcttgttc gacgcgcctt
     1921 tcttcaacat gtctccacgt gaagcccaag ttgccgatcc gcagatgcgt ctggctttag
     1981 tcacggcatt cgaagcgctc gagcgtgctg gttacgtcgg caaccgcact tcgtccacca
     2041 agctagaacg tatcggcacc tactacggcc aggccgctga cgattatcga gaggtcaacc
     2101 aagggcaaga ggtcagcact tactatattc caggaggttg tcgtgcattc ggtcctggtc
     2161 gcatcaacta cttcttcaag tttgctggcc ctagttacag cattgatacg gcctgctcgt
     2221 ctggactggc ggctattgag gtcgcttgtc aagcgctttg gcaaggtgat gtcgatatgg
     2281 ctgtcgcggg cggcgtcaat gtgctcacaa atcctgatgg ctttgctggc ctgggccacg
     2341 gtcacttcct gaccaaaggg cataatgcat gcaaaacctg ggacgccacc gctgatggat
     2401 actgtcgcgc agacggtgtg ggatctgtcg tcatcaaacg gcttcaagac gcagaggctg
     2461 acaacgacaa catcttggga gtaatactgg gtgccggaac caatcattct gccgaggctg
     2521 tttctatcac tcatcctcac gccggtcatc aagcatactt ggcccgacag gtgcttcgtc
     2581 aatctggtgt tgatccattg gatgtttctt atgttgagct ccatggcacc ggcacccagg
     2641 ctggcgacta cgaggagatg caaggcataa tggacgtgta cgctccgctc gtcaagcggc
     2701 gcagcaagga tcagcctctg cacattggcg ctgtcaaggc aaatgtcggt catggcgagt
     2761 ccgttgcggg gacgacagct ttgatcaagg tgctcttgat gttgcagaag aacggcattc
     2821 caccacatat cggcattaaa acggagatca accctcgtct tcccaaggat tttgacaagc
     2881 gcaacgtcca tatcccgttc gagatgacac agtggaagca gaaacccagc acaaagcgtg
     2941 tcgttgctct caacaacttt ggtgccgctg gtggcaatac aaccatgatt ttggaagagg
     3001 caccgactcg cacaacaaca gggagagatc cacgccctac ccaggtcatt gccgtgtcgg
     3061 cgaaaagcaa ggcatcattg gcaggaaaca ttgagcgcct gatcgcttac atcgatgcca
     3121 atccacagct caacctcgct gatctctcat acacgaccac cgcacgtcgc taccactacc
     3181 gcaaccgtct gacagtgacc gccccggacg ccgctcattt aaagtcacag ctcacttccc
     3241 gtctggcgaa gattgactcc gtcaaggccc tggcacagtc cgatccacca ttgattgctt
     3301 tcaccttcac cggccaaggc gcttcgaaca agtccatgga cctcgaattg taccgcgacg
     3361 ttccgatctt ccgcgaacat attcagcatt tggatcatct ggcccaaggc caaggctttc
     3421 catctttcat tccggctctc gacggatcac atccgaagga tcatgcacat tccccggttg
     3481 tgactcagct tgctctggtg tgctccgaga tggcgctggc taaatactgg gctgctctgg
     3541 gcgtcaagcc ggatgtcgtc ctcggtcaca gtttgggcga atacgccgca atgcacgtgg
     3601 ccggcgtcgt ctctgccgct gacaccatat tcatggtagg acgccgggcc cagatgctgc
     3661 aagaaaaatg cacaattgga agccacacca tgatggccgt ccgagcctct cttgccacca
     3721 ttgcccagaa ctcgacgaat aagccgtata ccgttgcttg tgtcaatgga ccttcggaca
     3781 cagtgctcag tggcaccaag cagcagatgg atgaggtcgc tgtgccactc gagaaggctg
     3841 gattgcgctg cattaagctc gacgtggcgt ttgcattcca ttctgaacag actgacccta
     3901 tcctggacga gttcgaggca acggtgaaga cgggtgtgat tttccaggag cccaaagtcc
     3961 ccatcatctc gcccttgctc ggcagagtcg ttttcgacga caagaccttc aatgcgaact
     4021 acgtccgccg agccacgaga gaagccgtga gattcttccc tgcactggag aacgcacaag
     4081 aggtttcgac catcaatgac gagactatct gggtagagat tggccctcat cctgtttgca
     4141 ccaattttgt caaaacctcc atcccatcca cagccgtcgc cgtcccctcc ttccggcgcg
     4201 gcgaggacaa ctgggctacc atggcagaag caatagccac attgcacgtc gcgggtgtca
     4261 ccgttggctg gaacgagttt catcacccct tcgaaagcag actccgcctc ctagacttgc
     4321 ctacatacgc ctggaacgag aagaacttct ggctccagta caacggcgac tggtgtttga
     4381 ccaagggcaa tactttttac gcggccgaga aatcggctgc aacaggacag acgatctcaa
     4441 aaccactgcc tgtcagcgac ttgcagactt cgacagtaca gcaagtcgtc agggaggtcg
     4501 ttgatggctc cgctggtaca gtgatcatgc aatccgatct catgcaaccg gattttcttg
     4561 ctgcagctca tggtcatcgc atgaacgatt gcggcgttgt cacatcgtcc atccatgccg
     4621 atatcgctta taccctcggt agctatttgt accgcaaatt ctatcccaag tcaaaggacg
     4681 tcaacatgaa catcgcggac ctggtggtca caaagggcct cgtcgcccaa agtaacaaga
     4741 agctgcctca gttgatccag gtcaccgccg ccacggccaa catcgaatcc ggtgttgttg
     4801 aattgagttg gcaaaacgtt gacaacgccg gtgacgtgca tgagccattt gccacggcca
     4861 agatcgtcta cggcgatgct tctgcgtggc tcagctcctg gctccctctg acgcacttga
     4921 ttcacagccg gatcgaggcg ctggagcaga tggcggcaaa aggccaagcc agccgatttt
     4981 cgcggaacat ggcatacaca ctcttcgccg ccaatctcgt cgactatgcc gacaagtatc
     5041 gcggcatgca atccgtcgtg atgcacgatc tggaggcatt tgccgacgtg cagctgacta
     5101 ccaaggaaag cggcacctgg accgtggcgc cctatttcat cgacagcgtg gcccatctgg
     5161 ctggattcat catgaactgc tccgacgcga tggatgtccg gaacaactac tgcgtcacac
     5221 ctggatggaa gtcgatgcaa tttgcaaagc ctctcatccc gggagcaaag taccggtcct
     5281 acgtcaagat gatccccact gctgaggacc ccaccatctt cttgggcgac gtgtacatca
     5341 tgcaagacaa cgccatcatg ggcatggtgg gtggcatcca attccgccgc tacccgcgga
     5401 tcttgctcag ccgattcttt tccccaccag ataagatggc ggcgatggag gacaaagcgg
     5461 tgaaagttgc agctccgcaa gccctactcc cagccgcagc ccacggaaag ccagcgacta
     5521 ccacgcagca gcaactgcca aagccgacac gcttcagtgc tgacgatgtc atccatccga
     5581 ctgccgtccc tggaccgccg aaggacttca aagccgccac cactgttgtt gtggaagtct
     5641 cgccgagcag tagcgaatcc gagacgtcaa gtgatgataa cagcatcgtc agcaaagccc
     5701 ttgagctcat tgcgaatgag ggcgcgttgg acattgctga cctggaagat gatgtccggt
     5761 tcgcggacct gggcgtcgac agtctcatgt cgctggtgct cgcggagaaa ttcagagtcg
     5821 aacttgatat caaggttggt ggtggccttt tcgtggacta ccctaccgtc ggggacctgt
     5881 gcaagtggct ggcggagtat tacggctagg gagattgata agacttttct agttgcatat
     5941 cttttatatt taaatcttat ctattagtta attttttgta atttatcctt atatatagtc
     6001 tggttattct aaaatatcat ttcagtatct aaaaattccc ctcttttttc agttatatct
     6061 taacaggcga cagtccaaat gttgatttat cccagtccga ttcatcaggg ttgtgaagca
     6121 ttttgtcaat ggtcgaaatc acatcagtaa tagtgcctct tacttgcctc atagaatttc
     6181 tttctcttaa cgtcaccgtt tggtctttta tagtttcgaa atctatggtg ataccaaatg
     6241 gtgttcccaa ttcatcgtta cgggcgtatt ttttaccaat tgaagtattg gaatcgtcaa
     6301 ttttaaagta tatctctctt ttacgtaaag cctgcgagat cctcttaagt atagcgggga
     6361 agccatcgtt attcgatatt gtcgtaacaa atactttgat cggcgctatg cggccgccac
     6421 cgcggtggag ctccagcttt tgttcccttt agtgagggtt aattgcgcgc ttggcgagac
     6481 tcaaacaaga tatattttgc ccgccttaca gacgaaacta gttggaggtt atggagcata
     6541 ctatcacgtg ggcggccact ggtgagttac tacaccccag gggcaacgtt gatgctccta
     6601 aaaaactctg gctggacgca agccgtaaca cccgtgtcac ttcataatcg tttgcaattc
     6661 agggcttgat ctacacatta ccctgttatc cctattaatt aatacggtta tccacagaat
     6721 caggggataa cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta
     6781 aaaaggccgc gttgctggcg tttttccata ggctccgccc ccctgacgag catcacaaaa
     6841 atcgacgctc aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc
     6901 cccctggaag ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt
     6961 ccgcctttct cccttcggga agcgtggcgc tttctcatag ctcacgctgt aggtatctca
     7021 gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg
     7081 accgctgcgc cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat
     7141 cgccactggc agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta
     7201 cagagttctt gaagtggtgg cctaactacg gctacactag aaggacagta tttggtatct
     7261 gcgctctgct gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac
     7321 aaaccaccgc tggtagcggt ggtttttttg tttgcaagca gcagattacg cgcagaaaaa
     7381 aaggatctca agaagatcct ttgatctttt ctacggggtc tgacgctcag tggaacgaaa
     7441 actcacgtta agggattttg gtcatgagat tatcaaaaag gatcttcacc tagatccttt
     7501 taaattaaaa atgaagtttt aaatcaatct aaagtatata tgagtaaact tggtctgaca
     7561 gttaccaatg cttaatcagt gaggcaccta tctcagcgat ctgtctattt cgttcatcca
     7621 tagttgcctg actccccgtc gtgtagataa ctacgatacg ggagggctta ccatctggcc
     7681 ccagtgctgc aatgataccg cgagacccac gctcaccggc tccagattta tcagcaataa
     7741 accagccagc cggaagggcc gagcgcagaa gtggtcctgc aactttatcc gcctccatcc
     7801 agtctattaa ttgttgccgg gaagctagag taagtagttc gccagttaat agtttgcgca
     7861 acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat
     7921 tcagctccgg ttcccaacga tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag
     7981 cggttagctc cttcggtcct ccgatcgttg tcagaagtaa gttggccgca gtgttatcac
     8041 tcatggttat ggcagcactg cataattctc ttactgtcat gccatccgta agatgctttt
     8101 ctgtgactgg tgagtactca accaagtcat tctgagaata gtgtatgcgg cgaccgagtt
     8161 gctcttgccc ggcgtcaata cgggataata ccgcgccaca tagcagaact ttaaaagtgc
     8221 tcatcattgg aaaacgttct tcggggcgaa aactctcaag gatcttaccg ctgttgagat
     8281 ccagttcgat gtaacccact cgtgcaccca actgatcttc agcatctttt actttcacca
     8341 gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga ataagggcga
     8401 cacggaaatg ttgaatactc atactcttcc tttttcaata ttattgaagc atttatcagg
     8461 gttattgtct catgagcgga tacatatttg aatgtattta gaaaaataaa caaatagggg
     8521 ttccgcgcac atttccccga aaagtgccac ctgaacgaag catctgtgct tcattttgta
     8581 gaacaaaaat gcaacgcgag agcgctaatt tttcaaacaa agaatctgag ctgcattttt
     8641 acagaacaga aatgcaacgc gaaagcgcta ttttaccaac gaagaatctg tgcttcattt
     8701 ttgtaaaaca aaaatgcaac gcgagagcgc taatttttca aacaaagaat ctgagctgca
     8761 tttttacaga acagaaatgc aacgcgagag cgctatttta ccaacaaaga atctatactt
     8821 cttttttgtt ctacaaaaat gcatcccgag agcgctattt ttctaacaaa gcatcttaga
     8881 ttactttttt tctcctttgt gcgctctata atgcagtctc ttgataactt tttgcactgt
     8941 aggtccgtta aggttagaag aaggctactt tggtgtctat tttctcttcc ataaaaaaag
     9001 cctgactcca cttcccgcgt ttactgatta ctagcgaagc tgcgggtgca ttttttcaag
     9061 ataaaggcat ccccgattat attctatacc gatgtggatt gcgcatactt tgtgaacaga
     9121 aagtgatagc gttgatgatt cttcattggt cagaaaatta tgaacggttt cttctatttt
     9181 gtctctatat actacgtata ggaaatgttt acattttcgt attgttttcg attcactcta
     9241 tgaatagttc ttactacaat ttttttgtct aaagagtaat actagagata aacataaaaa
     9301 atgtagaggt cgagtttaga tgcaagttca aggagcgaaa ggtggatggg taggttatat
     9361 agggatatag cacagagata tatagcaaag agatactttt gagcaatgtt tgtggaagcg
     9421 gtattcgcaa tattttagta gctcgttaca gtccggtgcg tttttggttt tttgaaagtg
     9481 cgtcttcaga gcgcttttgg ttttcaaaag cgctctgaag ttcctatact ttctagagaa
     9541 taggaacttc ggaataggaa cttcaaagcg tttccgaaaa cgagcgcttc cgaaaatgca
     9601 acgcgagctg cgcacataca gctcactgtt cacgtcgcac ctatatctgc gtgttgcctg
     9661 tatatatata tacatgagaa gaacggcata gtgcgtgttt atgcttaaat gcgtacttat
     9721 atgcgtctat ttatgtagga tgaaaggtag tctagtacct cctgtgatat tatcccattc
     9781 catgcggggt atcgtatgct tccttcagca ctacccttta gctgttctat atgctgccac
     9841 tcctcaattg gattagtctc atccttcaat gctatcattt cctttgatat tggatcatac
     9901 taagaaacca ttattatcat gacattaacc tataaaaata ggcgtatcac gaggcccttt
     9961 cgtctcgcgc gtttcggtga tgacggtgaa aacctcggcg cgccattacc ctgttatccc
    10021 taatatacaa cgcaaagaag ctggaaaatt ggcgagggaa tcctgtttct gtctatccaa
    10081 gaatgggcat gaggtggcaa ccgtcgtgct agcgtacagg gtgcactttg taaccatttg
    10141 ggacaccgga cactcgctgt tttcgaaatt accctttaag cgcgggtatt gaaccaggct
    10201 tatgcccaag atcgtagcaa gcatttaact gtgggaatac tcaggtatcg taagatgcaa
    10261 gagttcgaat ctcttagcaa ccattatttt tttcctcaac ataacgagaa cacacagggg
    10321 cgctatcgca cagaatcaaa ttcgatgact ggaaattttt tgttaatttc agaggtcgcc
    10381 tgacgcatat acctttttca actgaaaaat tgggagaaaa aggaaaggtg agaggccgga
    10441 accggctttt catatagaat agagaagcgt tcatgactaa atgcttgcat cacaatactt
    10501 gaagttgaca atattattta aggacctatt gttttttcca ataggtggtt agcaatcgtc
    10561 ttactttcta acttttctta ccttttacat ttcagcaata tatatatata tttcaaggat
    10621 ataccattct aatgtctgcc cctatgtctg cccctaagaa gatcgtcgtt ttgccaggtg
    10681 accacgttgg tcaagaaatc acagccgaag ccattaaggt tcttaaagct atttctgatg
    10741 ttcgttccaa tgtcaagttc gatttcgaaa atcatttaat tggtggtgct gctatcgatg
    10801 ctacaggtgt cccacttcca gatgaggcgc tggaagcctc caagaaggtt gatgccgttt
    10861 tgttaggtgc tgtggctggt cctaaatggg gtaccggtag tgttagacct gaacaaggtt
    10921 tactaaaaat ccgtaaagaa cttcaattgt acgccaactt aagaccatgt aactttgcat
    10981 ccgactctct tttagactta tctccaatca agccacaatt tgctaaaggt actgacttcg
    11041 ttgttgtcag agaattagtg ggaggtattt actttggtaa gagaaaggaa gacgatggtg
    11101 atggtgtcgc ttgggatagt gaacaataca ccgttccaga agtgcaaaga atcacaagaa
    11161 tggccgcttt catggcccta caacatgagc caccattgcc tatttggtcc ttggataaag
    11221 ctaatctttt ggcctcttca agattatgga gaaaaactgt ggaggaaacc atcaagaacg
    11281 aattccctac attgaaggtt caacatcaat tgattgattc tgccgccatg atcctagtta
    11341 agaacccaac ccacctaaat ggtattataa tcaccagcaa catgtttggt gatatcatct
    11401 ccgatgaagc ctccgttatc ccaggttcct tgggtttgtt gccatctgcg tccttggcct
    11461 ctttgccaga caagaacacc gcatttggtt tgtacgaacc atgccacggt tctgctccag
    11521 atttgccaaa gaataaggtt gaccctatcg ccactatctt gtctgctgca atgatgttga
    11581 aattgtcatt gaacttgcct gaagaaggta aggccattga agatgcagtt aaaaaggttt
    11641 tggatgcagg tatcagaact ggtgatttag gtggttccaa cagtaccacc gaagtcggtg
    11701 atgctgtcgc cgaagaagtt aagaaaatcc ttgcttaaaa agattctctt tttttatgat
    11761 atttgtacat aaactttata aatgaaattc ataatagaaa cgacacgaaa ttacaaaatg
    11821 gaatatgttc atagggtaga cgaaactata tacgcaatct acatacattt atcaagaagg
    11881 agaaaaagga ggatagtaaa ggaatacagg taagcaaatt gatactaatg gctcaacgaa
    11941 atgacggtat cgataagctt gatatcgaat tcctgcagcc cgggggatcc actagttcta
    12001 gagttaatta a
//
