LOCUS       sequence-208205-       12044 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of a fungal biosynthetic gene cluster.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12044)
  AUTHORS   Harvey CJB, Tang M, Schlecht U, Horecka J, Fischer CR, Lin HC, Li J,
            Naughton B, Cherry J, Miranda M, Li YF, Chu AM, Hennessy JR, Vandova
            GA, Inglis D, Aiyar RS, Steinmetz LM, Davis RW, Medema MH, Sattely 
            E, Khosla C, St Onge RP, Tang Y, Hillenmeyer ME
  TITLE     HEx: A heterologous expression platform for the discovery of fungal 
            natural products.
  JOURNAL   Sci Adv. 2018 Apr 11;4(4):eaar5459. doi: 10.1126/sciadv.aar5459. 
            eCollection 2018 Apr.
  PUBMED    29651464
REFERENCE   2  (bases 1 to 12044)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Adv."; 
            date: "2018-04-11"; volume: "4(4):eaar5459. doi"; pages: " 
            10.1126/sciadv.aar5459. eCollection 2018 Apr"
COMMENT     Sequence Label: pCHSU68.1-2.2
FEATURES             Location/Qualifiers
     source          1..12044
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             504..527
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             complement(5584..5595)
                     /codon_start=1
                     /product="WELQut protease recognition and cleavage site"
                     /label=WELQut site
                     /translation="WELQ"
     primer_bind     complement(6479..6499)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(6479..6497)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     rep_origin      complement(6836..7424)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6916..6935)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7595..8386,8387..8455))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8218..8237
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8456..8560)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      8587..9929
                     /label=2u ori
                     /note="yeast 2u plasmid origin of replication"
     protein_bind    complement(9550..9597)
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     primer_bind     9970..9988
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        10260..10664
                     /gene="S. cerevisiae LEU2"
                     /label=LEU2 promoter
     CDS             10677..11771
                     /codon_start=1
                     /gene="S. cerevisiae LEU2"
                     /product="3-isopropylmalate dehydrogenase, required for 
                     leucine biosynthesis"
                     /label=LEU2
                     /note="yeast auxotrophic marker"
                     /translation="MSAPKKIVVLPGDHVGQEITAEAIKVLKAISDVRSNVKFDFENHL
                     IGGAAIDATGVPLPDEALEASKKVDAVLLGAVAGPKWGTGSVRPEQGLLKIRKELQLYA
                     NLRPCNFASDSLLDLSPIKPQFAKGTDFVVVRELVGGIYFGKRKEDDGDGVAWDSEQYT
                     VPEVQRITRMAAFMALQHEPPLPIWSLDKANLLASSRLWRKTVEETIKNEFPTLKVQHQ
                     LIDSAAMILVKNPTHLNGIIITSNMFGDIISDEASVIPGSLGLLPSASLASLPDKNTAF
                     GLYEPCHGSAPDLPKNKVDPIATILSAAMMLKLSLNLPEEGKAIEDAVKKVLDAGIRTG
                     DLGGSNSTTEVGDAVAEEVKKILA"
     primer_bind     complement(12019..12035)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(12019..12035)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
ORIGIN
        1 tatctaaaaa ttgccttatg atccgtctct ccggttacag cctgtgtaac tgattaatcc
       61 tgcctttcta atcaccattc taatgtttta attaagggat tttgtcttca ttaacggctt
      121 tcgctcataa aaatgttatg acgttttgcc cgcaggcggg aaaccatcca cttcacgaga
      181 ctgatctcct ctgccggaac accgggcatc tccaacttat aagttggaga aataagagaa
      241 tttcagattg agagaatgaa aaaaaaaaaa aaaaaaaagg cagaggagag catagaaatg
      301 gggttcactt tttggtaaag ctatagcatg cctatcacat ataaatagag tgccagtagc
      361 gacttttttc acactcgaaa tactcttact actgctctct tgttgttttt atcacttctt
      421 gtttcttctt ggtaaataga atatcaagct acaaaaagca tacaatcaac tatcaactat
      481 taactatatc gtaatacaca atggattata aggatgatga tgataagact agtatgacta
      541 tctacacccc aacatctggt ggagagctcg acatgggctt gccaggagtg aaactcatat
      601 atttcggcaa cgagtttccc aaagatgact tggaagacct ttttcgccgc atacacaacc
      661 acagtaaaga caataatcac tttatactaa ctcaattcat aaacgaggct acattggcaa
      721 tcaaggacga gatccggcgc ctaccgactg aaatgaaaca aagtataccc ccttttgaca
      781 ccattttgag atgggccgag cctaacgagc ttcgaaatgg cttgatgtgt ggtgctgtcg
      841 atggtgttct tctggtagtc gctcagctag cggcgtatat cggatatatt gagaattctt
      901 ccagggagtt taacgacagt acaaacttat gtcttgctgg ccttggaatt ggcttactag
      961 catcgaccgc aatttctttg tccaaaacgc tcgtcgacat cccacttgct ggcgcagatg
     1021 tcgttcgttt ggcgtttcgt ctggggattc atgtcatggg cgtatccgag aatctagaag
     1081 ctcgtgattt gtcagagaca ccaagtacat gggcttacgt ggtgcataac gccgattcag
     1141 atgatgtcca gagtgaacta gattttatgc actctcgttc agaaactccg gacactggta
     1201 agatttttat cagtgctgta agccgcactt ctgtgacagt gagtgggcca ccaacaaggt
     1261 tgaatgctat tttcaacaaa tcgcaattct ttcgagaaaa gagattcatt gccctgcctg
     1321 tttatggtgg attatgccat gctcctcacg tttacggagt ggcagatact cacagcattg
     1381 tccacgggac ccatttgaaa acggtcaggg caaacttttc accaacgatt ccagtctact
     1441 ccaccagcac tggtctgcca tacccattca agactgccgc ggacattttt gaaagcgtcg
     1501 tgatggagct cctcacaaag gccatttatt gggaccgtgt cattcatggt ttggtagacc
     1561 gaatgaagga aaatgcgaca tccgaagccg ttctattcag ctttggcaat tccatacctc
     1621 tcaacgacct ccagactgca atcaaaaatg atgtgccctc cgtcgaactc tcaaccaaga
     1681 gtctgatctc atggcctttc catttgaatc ataacgactt tacacctcgc accccagccc
     1741 aagctaagct ggccatcatt ggcatgtctt gtcgacttcc gggaggtgct acaaacacag
     1801 aaaagttttg ggggctttta gaaaatggcc ttgatgtttc tcgaagaata ccagctgata
     1861 ggttcgatat tgagacacac tttgatccaa cgggcaagga cctaaacaag accatgactc
     1921 aatacggctg cttcattgaa gaacctggct tgtttgacgc cccttttttc aatatgtccc
     1981 ctcgtgaagc acaggttgta gatccgcaga tgcgcctttc tttggtcact gcgtatgagg
     2041 ctctggagcg cgctggctat gttggtaacc gcaccgcttc tacgcggttg gaacgtattg
     2101 gcacatacta tggccaagct gccgatgatt accgcgaagt caaccaaggt caggaggtca
     2161 gtacttacta tattccaggt ggctgtcgag cctttgggcc tggccggata aattatttct
     2221 tcaaatttgc aggtcccagc tacagtattg atactgcttg ctcctccggt cttgcggcga
     2281 ttgaggttgc ctgtcaggct atttggagtg gtgctgttga tatggctgtt gcagggggtg
     2341 tcaatgtgct aaccaaccct gatggcttta ctggattatg caaaggccac ttccttactc
     2401 agggacacaa cgcgtgcaaa acttgggatg caaccgccga cggatattgc agagctgacg
     2461 gtgtaggctc ggtcgtaatc aagaaactcg aggatgcaga ggcagataac gataacatct
     2521 tgggagttat tctgggtgcg ggaacaaacc attctgcgga agcagtctcc attactcacc
     2581 cccatgcagg ccatcaagcc tacctatctc gccaggtcct cggaaaatgt ggagtgaatc
     2641 ctttggatgt gtcatatatc gaacttcatg ggactggcac tcaagctggt gatcatgaag
     2701 agatgcaggg aatcatggaa gtatatgcac cactgaccaa acgccgcaat aaagaccagc
     2761 ctctgcatat cgggtctgtt aaagcgaaca ttggacatgg ggaatctgtt gcaggcgttt
     2821 catcattgat caaagtactg ctcatgctcc aaaagaatgc catcccgccc catattggta
     2881 tcaaaacgaa catcaacccc aagtttaaag atctcgataa gcgcaacttg catatacctt
     2941 ttcagaagac cccttggccc acagttccag gtaagagacg acttttagca ctcaacaatt
     3001 ttggcgccgc tggtggcaac acgacgatgg tcctagaaga agccccggat cgtgttattc
     3061 tagagaaaga ccatcgtcca aaccatgtta ttacagtttc agccaaaact aaaggatctt
     3121 tgaccgggaa tatagagcgc cttgttgcct atcttgatgg acatccagat acaaacatcg
     3181 cggatctggc atacacaacg aatgcgcgtc gctatcacca cagccatcgt gtagcaatag
     3241 ccacatctga tatagcacat ctcacaaaac agctcaaatc tcgtttagag aaggtcgatt
     3301 tggtaaatcc tgttaaaagc tccgaaccgc caccagttgc atttgtgttc actggtcagg
     3361 gcgcttctta caaatcaatg agcttggaat tgtatcgcga tatcccgaca tttcaacagc
     3421 acatccaaca tcttgactca ctggctcgtg gacaagggtt tccctcattt attccggcac
     3481 ttgatggctc gtttcccaaa gaccatgcgc attccccagt cgttacacac ttggcattgg
     3541 cttgcacgga gatagccatg gccaaatatt ggatttctct aggggttaag ccagatgttg
     3601 ttctgggcca cagtctgggt gaatatgcgg cgatgcacat agctggtgta gtcactgcaa
     3661 gtgataccat atttatggtg ggacgcaggg cacaaatgct ggaggagagg tgcaaaatcg
     3721 gcagtcatgc tatgatggcg gtacgagcct ccctgtctca aatagacgaa gtctgtgctg
     3781 gcaagtcata taagtacact attgcctgtg tcaacagtcc ttttgataca gtccttagcg
     3841 gtaccaatca tcaaattgat gacctagcgt cggtgctcga gggtgtcggt taccgctgta
     3901 ttaagctgga cgtggctttt gcatttcact ccgaacagac tgatcctatt ttggacgatt
     3961 ttgaagctat ttcaaagacc ggcgttgtct tctcagagcc aaaagtaccc gtaatatcgc
     4021 cattacttgg aaaagttgtt tttgacggca aaactttcag cgcaaattac gtccggcggg
     4081 caactcgaga gacggtagat ttttcatcgg cgttagagag tgctcggatg atttctacga
     4141 ttagcgatga cactatttgg attgaaattg ggcctcaccc ggtctgtgtt ggctttgtca
     4201 agtctacaat cccatctacc caactcacag ttgcatcgat gcgccgtggt gaggacaatt
     4261 ggaaaaccat ggcggaagcc atggcttctt tgcacgttgc cagcgtggaa gttggttgga
     4321 atgaatttca tcgtccgttc gagagcaaac tccggcttct tgatctgccg acttatgctt
     4381 ggaacgaaaa gaactactgg ttgcagtata acggtgactg gtgcttgaca aagggcaata
     4441 ccttctacac caaggagcag tcgacccagg ccaaatcaac catcaagtcc ctaccacgaa
     4501 gtgaaatcca gacaacatct gttcagagaa ttattgagca gacgttcgag gggccaactg
     4561 gtacggtggt tatgcagtca gaccttatgc agactgatct actcgctgct gctcatggac
     4621 atcgaatgaa caactgcggc gtcgtaacat cctcaatcca cgccgatatc gcatacactc
     4681 ttggtgatta cctataccgc aagctagttt ccaaacccga gcaagccaat atgaatattg
     4741 ccaatcttgt cgtgacaaag ggccttgtgg ctcaaagcaa taacgaagtg tctcaactga
     4801 ttcgagtgac tgctgttacg tctaatgttc acgccggcaa ggtggaattg acatggcaaa
     4861 atgttgacaa tgacggaaac gttcacgagc cgtttgctac tgcagaactt cattatgaaa
     4921 gagcatccga atggatttct tcttggactc ccgtccatca tcttgtgcaa ggccgaatcg
     4981 aggcccttga gcgcttggcc gtcgaaggca aagccaaccg cttttcgcgc agtatggcat
     5041 atactttatt cgccaacaat ttggttgatt atgaagacaa atatcgaggc atgcaatcag
     5101 ttgtcatgta cgagctggag gctttcgctg atgtgcaact taccacaaaa gagcgagggg
     5161 tttgggttat tcctccctat tttattgata gtttagcaca tttaggcggg tttgttatga
     5221 actgttctga cgcgatggac acgaaagaca atttttccgt tactccgggt tggaaatcca
     5281 tgcaattttt gaagccgttg cttccaggtg ccaaatatcg ttcgtatgtc aagatgattc
     5341 cgacaatcga ggacccaacc atcttcctag gcgacgttta tatcttgcaa gacaactcca
     5401 tcgtcgggat ggtgggtggc ctacagtttc ggcgttatcc acgtctgctg ctcaatcgat
     5461 tcttctcgcc gccggataga ataccagccg tgggtgatga accaacaccg cctgcaccaa
     5521 aggcttcagt atcaacacca gcacatcaaa cccaaaagtc aaaaccacgg tcaaagtcag
     5581 tttctgcagt tcccactcca gactccagca atgccgtgca gagccaacgc gatactccgt
     5641 tgcaggaagc tgctaccact ggcaatactg gccctgagcc agcatcgtct gctgatactg
     5701 catctgcttc aggcagtatc actgccaaag cgctgatgct tattgcccgg gaagcagcgt
     5761 tggatatctc agacttggag gatgatgcca gtttctcgca ccttggtatt gacagtttga
     5821 tgtcactcgt catagcagag aagtttatgg gggaactagg tgtcaaagtc ggtggcagtc
     5881 tgttcctgga ctacccgacc attggtgatt tgcgaaaatg gctggacgaa tactatgctt
     5941 gaggagattg ataagacttt tctagttgca tatcttttat atttaaatct tatctattag
     6001 ttaatttttt gtaatttatc cttatatata gtctggttat tctaaaatat catttcagta
     6061 tctaaaaatt cccctctttt ttcagttata tcttaacagg cgacagtcca aatgttgatt
     6121 tatcccagtc cgattcatca gggttgtgaa gcattttgtc aatggtcgaa atcacatcag
     6181 taatagtgcc tcttacttgc ctcatagaat ttctttctct taacgtcacc gtttggtctt
     6241 ttatagtttc gaaatctatg gtgataccaa atggtgttcc caattcatcg ttacgggcgt
     6301 attttttacc aattgaagta ttggaatcgt caattttaaa gtatatctct cttttacgta
     6361 aagcctgcga gatcctctta agtatagcgg ggaagccatc gttattcgat attgtcgtaa
     6421 caaatacttt gatcggcgct atgcggccgc caccgcggtg gagctccagc ttttgttccc
     6481 tttagtgagg gttaattgcg cgcttggcga gactcaaaca agatatattt tgcccgcctt
     6541 acagacgaaa ctagttggag gttatggagc atactatcac gtgggcggcc actggtgagt
     6601 tactacaccc caggggcaac gttgatgctc ctaaaaaact ctggctggac gcaagccgta
     6661 acacccgtgt cacttcataa tcgtttgcaa ttcagggctt gatctacaca ttaccctgtt
     6721 atccctatta attaatacgg ttatccacag aatcagggga taacgcagga aagaacatgt
     6781 gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc
     6841 ataggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa
     6901 acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc
     6961 ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg
     7021 cgctttctca tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc
     7081 tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc
     7141 gtcttgagtc caacccggta agacacgact tatcgccact ggcagcagcc actggtaaca
     7201 ggattagcag agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg tggcctaact
     7261 acggctacac tagaaggaca gtatttggta tctgcgctct gctgaagcca gttaccttcg
     7321 gaaaaagagt tggtagctct tgatccggca aacaaaccac cgctggtagc ggtggttttt
     7381 ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgatct
     7441 tttctacggg gtctgacgct cagtggaacg aaaactcacg ttaagggatt ttggtcatga
     7501 gattatcaaa aaggatcttc acctagatcc ttttaaatta aaaatgaagt tttaaatcaa
     7561 tctaaagtat atatgagtaa acttggtctg acagttacca atgcttaatc agtgaggcac
     7621 ctatctcagc gatctgtcta tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga
     7681 taactacgat acgggagggc ttaccatctg gccccagtgc tgcaatgata ccgcgagacc
     7741 cacgctcacc ggctccagat ttatcagcaa taaaccagcc agccggaagg gccgagcgca
     7801 gaagtggtcc tgcaacttta tccgcctcca tccagtctat taattgttgc cgggaagcta
     7861 gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt tgccattgct acaggcatcg
     7921 tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc cggttcccaa cgatcaaggc
     7981 gagttacatg atcccccatg ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg
     8041 ttgtcagaag taagttggcc gcagtgttat cactcatggt tatggcagca ctgcataatt
     8101 ctcttactgt catgccatcc gtaagatgct tttctgtgac tggtgagtac tcaaccaagt
     8161 cattctgaga atagtgtatg cggcgaccga gttgctcttg cccggcgtca atacgggata
     8221 ataccgcgcc acatagcaga actttaaaag tgctcatcat tggaaaacgt tcttcggggc
     8281 gaaaactctc aaggatctta ccgctgttga gatccagttc gatgtaaccc actcgtgcac
     8341 ccaactgatc ttcagcatct tttactttca ccagcgtttc tgggtgagca aaaacaggaa
     8401 ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa atgttgaata ctcatactct
     8461 tcctttttca atattattga agcatttatc agggttattg tctcatgagc ggatacatat
     8521 ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg cacatttccc cgaaaagtgc
     8581 cacctgaacg aagcatctgt gcttcatttt gtagaacaaa aatgcaacgc gagagcgcta
     8641 atttttcaaa caaagaatct gagctgcatt tttacagaac agaaatgcaa cgcgaaagcg
     8701 ctattttacc aacgaagaat ctgtgcttca tttttgtaaa acaaaaatgc aacgcgagag
     8761 cgctaatttt tcaaacaaag aatctgagct gcatttttac agaacagaaa tgcaacgcga
     8821 gagcgctatt ttaccaacaa agaatctata cttctttttt gttctacaaa aatgcatccc
     8881 gagagcgcta tttttctaac aaagcatctt agattacttt ttttctcctt tgtgcgctct
     8941 ataatgcagt ctcttgataa ctttttgcac tgtaggtccg ttaaggttag aagaaggcta
     9001 ctttggtgtc tattttctct tccataaaaa aagcctgact ccacttcccg cgtttactga
     9061 ttactagcga agctgcgggt gcattttttc aagataaagg catccccgat tatattctat
     9121 accgatgtgg attgcgcata ctttgtgaac agaaagtgat agcgttgatg attcttcatt
     9181 ggtcagaaaa ttatgaacgg tttcttctat tttgtctcta tatactacgt ataggaaatg
     9241 tttacatttt cgtattgttt tcgattcact ctatgaatag ttcttactac aatttttttg
     9301 tctaaagagt aatactagag ataaacataa aaaatgtaga ggtcgagttt agatgcaagt
     9361 tcaaggagcg aaaggtggat gggtaggtta tatagggata tagcacagag atatatagca
     9421 aagagatact tttgagcaat gtttgtggaa gcggtattcg caatatttta gtagctcgtt
     9481 acagtccggt gcgtttttgg ttttttgaaa gtgcgtcttc agagcgcttt tggttttcaa
     9541 aagcgctctg aagttcctat actttctaga gaataggaac ttcggaatag gaacttcaaa
     9601 gcgtttccga aaacgagcgc ttccgaaaat gcaacgcgag ctgcgcacat acagctcact
     9661 gttcacgtcg cacctatatc tgcgtgttgc ctgtatatat atatacatga gaagaacggc
     9721 atagtgcgtg tttatgctta aatgcgtact tatatgcgtc tatttatgta ggatgaaagg
     9781 tagtctagta cctcctgtga tattatccca ttccatgcgg ggtatcgtat gcttccttca
     9841 gcactaccct ttagctgttc tatatgctgc cactcctcaa ttggattagt ctcatccttc
     9901 aatgctatca tttcctttga tattggatca tactaagaaa ccattattat catgacatta
     9961 acctataaaa ataggcgtat cacgaggccc tttcgtctcg cgcgtttcgg tgatgacggt
    10021 gaaaacctcg gcgcgccatt accctgttat ccctaatata caacgcaaag aagctggaaa
    10081 attggcgagg gaatcctgtt tctgtctatc caagaatggg catgaggtgg caaccgtcgt
    10141 gctagcgtac agggtgcact ttgtaaccat ttgggacacc ggacactcgc tgttttcgaa
    10201 attacccttt aagcgcgggt attgaaccag gcttatgccc aagatcgtag caagcattta
    10261 actgtgggaa tactcaggta tcgtaagatg caagagttcg aatctcttag caaccattat
    10321 ttttttcctc aacataacga gaacacacag gggcgctatc gcacagaatc aaattcgatg
    10381 actggaaatt ttttgttaat ttcagaggtc gcctgacgca tatacctttt tcaactgaaa
    10441 aattgggaga aaaaggaaag gtgagaggcc ggaaccggct tttcatatag aatagagaag
    10501 cgttcatgac taaatgcttg catcacaata cttgaagttg acaatattat ttaaggacct
    10561 attgtttttt ccaataggtg gttagcaatc gtcttacttt ctaacttttc ttacctttta
    10621 catttcagca atatatatat atatttcaag gatataccat tctaatgtct gcccctatgt
    10681 ctgcccctaa gaagatcgtc gttttgccag gtgaccacgt tggtcaagaa atcacagccg
    10741 aagccattaa ggttcttaaa gctatttctg atgttcgttc caatgtcaag ttcgatttcg
    10801 aaaatcattt aattggtggt gctgctatcg atgctacagg tgtcccactt ccagatgagg
    10861 cgctggaagc ctccaagaag gttgatgccg ttttgttagg tgctgtggct ggtcctaaat
    10921 ggggtaccgg tagtgttaga cctgaacaag gtttactaaa aatccgtaaa gaacttcaat
    10981 tgtacgccaa cttaagacca tgtaactttg catccgactc tcttttagac ttatctccaa
    11041 tcaagccaca atttgctaaa ggtactgact tcgttgttgt cagagaatta gtgggaggta
    11101 tttactttgg taagagaaag gaagacgatg gtgatggtgt cgcttgggat agtgaacaat
    11161 acaccgttcc agaagtgcaa agaatcacaa gaatggccgc tttcatggcc ctacaacatg
    11221 agccaccatt gcctatttgg tccttggata aagctaatct tttggcctct tcaagattat
    11281 ggagaaaaac tgtggaggaa accatcaaga acgaattccc tacattgaag gttcaacatc
    11341 aattgattga ttctgccgcc atgatcctag ttaagaaccc aacccaccta aatggtatta
    11401 taatcaccag caacatgttt ggtgatatca tctccgatga agcctccgtt atcccaggtt
    11461 ccttgggttt gttgccatct gcgtccttgg cctctttgcc agacaagaac accgcatttg
    11521 gtttgtacga accatgccac ggttctgctc cagatttgcc aaagaataag gttgacccta
    11581 tcgccactat cttgtctgct gcaatgatgt tgaaattgtc attgaacttg cctgaagaag
    11641 gtaaggccat tgaagatgca gttaaaaagg ttttggatgc aggtatcaga actggtgatt
    11701 taggtggttc caacagtacc accgaagtcg gtgatgctgt cgccgaagaa gttaagaaaa
    11761 tccttgctta aaaagattct ctttttttat gatatttgta cataaacttt ataaatgaaa
    11821 ttcataatag aaacgacacg aaattacaaa atggaatatg ttcatagggt agacgaaact
    11881 atatacgcaa tctacataca tttatcaaga aggagaaaaa ggaggatagt aaaggaatac
    11941 aggtaagcaa attgatacta atggctcaac gaaatgacgg tatcgataag cttgatatcg
    12001 aattcctgca gcccggggga tccactagtt ctagagttaa ttaa
//
